                         SEQUENCE LISTING

<110>  ST. JUDE CHILDREN'S RESEARCH HOSPITAL, INC.
 
<120>  METHODS AND COMPOSITIONS FOR TREATING CD33+ CANCERS AND IMPROVING
        IN VIVO PERSISTENCE OF CHIMERIC ANTIGEN RECEPTOR T CELLS

<130>  243734.000102

<150>  62/577,494
<151>  2017-10-26

<160>  16    

<170>  PatentIn version 3.5

<210>  1
<211>  1508
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic polynucleotide

<400>  1
gaattcgccc ttccaccatg gccttaccag tgaccgcctt gctcctgccg ctggccttgc       60

tgctccacgc cgccaggccg gacattcaga tgacccagtc accatccagc ctcagtgctt      120

ctgttgggga ccgagttacc atcacttgtc gcgcaagcga atctgtcgac aactatggca      180

taagttttat gaattggttt cagcagaagc caggaaaggc accgaagctg cttatctacg      240

ctgcaagtaa tcaaggatcc ggcgtgccta gccggttctc cggatcagga tccgggacag      300

actttacatt gacaatttct agcctgcagc cagatgattt tgcaacctac tactgtcaac      360

agtctaagga agttccctgg acgtttgggc agggcaccaa ggttgagatc aagggcggcg      420

gcgggtccgg aggtggcggg tccggcggcg gtggctccgg gggggggggt agccaggtcc      480

agctcgtaca aagtggtgcc gaggtgaaga aacccgggag cagcgtgaaa gtgtcctgta      540

aggccagtgg ctacaccttt acagactaca atatgcactg ggtgcgccag gcgcccggcc      600

agggccttga gtggataggc tacatttatc cctacaatgg cggaaccgga tacaatcaga      660

agtttaagag caaggcaaca attaccgctg atgagtccac taacaccgct tacatggaat      720

tgagcagtct tcgatccgaa gacactgccg tgtattactg tgcccgagga agacctgcca      780

tggactactg gggtcaggga actctggtaa ccgtctctag cgcggccgca accacgacgc      840

cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg tccctgcgcc      900

cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg gacttcgcct      960

gtgatatcta catctgggcg cccttggccg ggacttgtgg ggtccttctc ctgtcactgg     1020

ttatcaccct ttactgcaaa cggggcagaa agaaactcct gtatatattc aaacaaccat     1080

ttatgagacc agtacaaact actcaagagg aagatggctg tagctgccga tttccagaag     1140

aagaagaagg aggatgtgaa ctgagagtga agttcagcag gagcgcagac gcccccgcgt     1200

accagcaggg ccagaaccag ctctataacg agctcaatct aggacgaaga gaggagtacg     1260

atgttttgga caagagacgt ggccgggacc ctgagatggg gggaaagccg agaaggaaga     1320

accctcagga aggcctgtac aatgaactgc agaaagataa gatggcggag gcctacagtg     1380

agattgggat gaaaggcgag cgccggaggg gcaaggggca cgatggcctt taccagggtc     1440

tcagtacagc caccaaggac acctacgacg cccttcacat gcaggccctg ccccctcgct     1500

aactcgag                                                              1508


<210>  2
<211>  499
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic polypeptide

<400>  2

Ile Arg Pro Ser Thr Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro 
1               5                   10                  15      


Leu Ala Leu Leu Leu His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln 
            20                  25                  30          


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
        35                  40                  45              


Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr Gly Ile Ser Phe Met Asn 
    50                  55                  60                  


Trp Phe Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile Tyr Ala 
65                  70                  75                  80  


Ala Ser Asn Gln Gly Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly 
                85                  90                  95      


Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Asp Asp 
            100                 105                 110         


Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Lys Glu Val Pro Trp Thr Phe 
        115                 120                 125             


Gly Gln Gly Thr Lys Val Glu Ile Lys Gly Gly Gly Gly Ser Gly Gly 
    130                 135                 140                 


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln Val Gln 
145                 150                 155                 160 


Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser Ser Val Lys 
                165                 170                 175     


Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp Tyr Asn Met His 
            180                 185                 190         


Trp Val Arg Gln Ala Pro Gly Gln Gly Leu Glu Trp Ile Gly Tyr Ile 
        195                 200                 205             


Tyr Pro Tyr Asn Gly Gly Thr Gly Tyr Asn Gln Lys Phe Lys Ser Lys 
    210                 215                 220                 


Ala Thr Ile Thr Ala Asp Glu Ser Thr Asn Thr Ala Tyr Met Glu Leu 
225                 230                 235                 240 


Ser Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gly 
                245                 250                 255     


Arg Pro Ala Met Asp Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser 
            260                 265                 270         


Ser Ala Ala Ala Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
        275                 280                 285             


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
    290                 295                 300                 


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
305                 310                 315                 320 


Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu 
                325                 330                 335     


Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu 
            340                 345                 350         


Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln 
        355                 360                 365             


Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly 
    370                 375                 380                 


Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr 
385                 390                 395                 400 


Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg 
                405                 410                 415     


Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met 
            420                 425                 430         


Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu 
        435                 440                 445             


Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys 
    450                 455                 460                 


Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu 
465                 470                 475                 480 


Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu 
                485                 490                 495     


Pro Pro Arg 
            


<210>  3
<211>  247
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic polypeptide

<400>  3

Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Glu Ser Val Asp Asn Tyr 
            20                  25                  30          


Gly Ile Ser Phe Met Asn Trp Phe Gln Gln Lys Pro Gly Lys Ala Pro 
        35                  40                  45              


Lys Leu Leu Ile Tyr Ala Ala Ser Asn Gln Gly Ser Gly Val Pro Ser 
    50                  55                  60                  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser 
65                  70                  75                  80  


Ser Leu Gln Pro Asp Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Ser Lys 
                85                  90                  95      


Glu Val Pro Trp Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys Gly 
            100                 105                 110         


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
        115                 120                 125             


Gly Gly Ser Gln Val Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys 
    130                 135                 140                 


Pro Gly Ser Ser Val Lys Val Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
145                 150                 155                 160 


Thr Asp Tyr Asn Met His Trp Val Arg Gln Ala Pro Gly Gln Gly Leu 
                165                 170                 175     


Glu Trp Ile Gly Tyr Ile Tyr Pro Tyr Asn Gly Gly Thr Gly Tyr Asn 
            180                 185                 190         


Gln Lys Phe Lys Ser Lys Ala Thr Ile Thr Ala Asp Glu Ser Thr Asn 
        195                 200                 205             


Thr Ala Tyr Met Glu Leu Ser Ser Leu Arg Ser Glu Asp Thr Ala Val 
    210                 215                 220                 


Tyr Tyr Cys Ala Arg Gly Arg Pro Ala Met Asp Tyr Trp Gly Gln Gly 
225                 230                 235                 240 


Thr Leu Val Thr Val Ser Ser 
                245         


<210>  4
<211>  255
<212>  PRT
<213>  Homo sapiens

<400>  4

Met Gly Asn Ser Cys Tyr Asn Ile Val Ala Thr Leu Leu Leu Val Leu 
1               5                   10                  15      


Asn Phe Glu Arg Thr Arg Ser Leu Gln Asp Pro Cys Ser Asn Cys Pro 
            20                  25                  30          


Ala Gly Thr Phe Cys Asp Asn Asn Arg Asn Gln Ile Cys Ser Pro Cys 
        35                  40                  45              


Pro Pro Asn Ser Phe Ser Ser Ala Gly Gly Gln Arg Thr Cys Asp Ile 
    50                  55                  60                  


Cys Arg Gln Cys Lys Gly Val Phe Arg Thr Arg Lys Glu Cys Ser Ser 
65                  70                  75                  80  


Thr Ser Asn Ala Glu Cys Asp Cys Thr Pro Gly Phe His Cys Leu Gly 
                85                  90                  95      


Ala Gly Cys Ser Met Cys Glu Gln Asp Cys Lys Gln Gly Gln Glu Leu 
            100                 105                 110         


Thr Lys Lys Gly Cys Lys Asp Cys Cys Phe Gly Thr Phe Asn Asp Gln 
        115                 120                 125             


Lys Arg Gly Ile Cys Arg Pro Trp Thr Asn Cys Ser Leu Asp Gly Lys 
    130                 135                 140                 


Ser Val Leu Val Asn Gly Thr Lys Glu Arg Asp Val Val Cys Gly Pro 
145                 150                 155                 160 


Ser Pro Ala Asp Leu Ser Pro Gly Ala Ser Ser Val Thr Pro Pro Ala 
                165                 170                 175     


Pro Ala Arg Glu Pro Gly His Ser Pro Gln Ile Ile Ser Phe Phe Leu 
            180                 185                 190         


Ala Leu Thr Ser Thr Ala Leu Leu Phe Leu Leu Phe Phe Leu Thr Leu 
        195                 200                 205             


Arg Phe Ser Val Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe 
    210                 215                 220                 


Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly 
225                 230                 235                 240 


Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
                245                 250                 255 


<210>  5
<211>  109
<212>  PRT
<213>  Homo sapiens

<400>  5

Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln 
1               5                   10                  15      


Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu 
            20                  25                  30          


Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg 
        35                  40                  45              


Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met 
    50                  55                  60                  


Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly 
65                  70                  75                  80  


Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp 
                85                  90                  95      


Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 


<210>  6
<211>  69
<212>  PRT
<213>  Homo sapiens

<400>  6

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
        35                  40                  45              


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
    50                  55                  60                  


Ile Thr Leu Tyr Cys 
65                  


<210>  7
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  7

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro 
            20      


<210>  8
<211>  741
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Description of Artificial Sequence: Synthetic polynucleotide

<400>  8
gacattcaga tgacccagtc accatccagc ctcagtgctt ctgttgggga ccgagttacc       60

atcacttgtc gcgcaagcga atctgtcgac aactatggca taagttttat gaattggttt      120

cagcagaagc caggaaaggc accgaagctg cttatctacg ctgcaagtaa tcaaggatcc      180

ggcgtgccta gccggttctc cggatcagga tccgggacag actttacatt gacaatttct      240

agcctgcagc cagatgattt tgcaacctac tactgtcaac agtctaagga agttccctgg      300

acgtttgggc agggcaccaa ggttgagatc aagggcggcg gcgggtccgg aggtggcggg      360

tccggcggcg gtggctccgg gggggggggt agccaggtcc agctcgtaca aagtggtgcc      420

gaggtgaaga aacccgggag cagcgtgaaa gtgtcctgta aggccagtgg ctacaccttt      480

acagactaca atatgcactg ggtgcgccag gcgcccggcc agggccttga gtggataggc      540

tacatttatc cctacaatgg cggaaccgga tacaatcaga agtttaagag caaggcaaca      600

attaccgctg atgagtccac taacaccgct tacatggaat tgagcagtct tcgatccgaa      660

gacactgccg tgtattactg tgcccgagga agacctgcca tggactactg gggtcaggga      720

actctggtaa ccgtctctag c                                                741


<210>  9
<211>  129
<212>  DNA
<213>  Homo sapiens

<400>  9
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa       60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt      120

gaactgaga                                                              129


<210>  10
<211>  333
<212>  DNA
<213>  Homo sapiens

<400>  10
agttcagcag gagcgcagac gcccccgcgt accagcaggg ccagaaccag ctctataacg       60

agctcaatct aggacgaaga gaggagtacg atgttttgga caagagacgt ggccgggacc      120

ctgagatggg gggaaagccg agaaggaaga accctcagga aggcctgtac aatgaactgc      180

agaaagataa gatggcggag gcctacagtg agattgggat gaaaggcgag cgccggaggg      240

gcaaggggca cgatggcctt taccagggtc tcagtacagc caccaaggac acctacgacg      300

cccttcacat gcaggccctg ccccctcgct aac                                   333


<210>  11
<211>  209
<212>  DNA
<213>  Homo sapiens

<400>  11
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgatatcta catctgggcg cccttggccg ggacttgtgg ggtccttctc      180

ctgtcactgg ttatcaccct ttactgcaa                                        209


<210>  12
<211>  63
<212>  DNA
<213>  Homo sapiens

<400>  12
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccg                                                                     63


<210>  13
<211>  45
<212>  PRT
<213>  Homo sapiens

<400>  13

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  45  


<210>  14
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  14

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr 
            20      


<210>  15
<211>  135
<212>  DNA
<213>  Homo sapiens

<400>  15
accacgacgc cagcgccgcg accaccaaca ccggcgccca ccatcgcgtc gcagcccctg       60

tccctgcgcc cagaggcgtg ccggccagcg gcggggggcg cagtgcacac gagggggctg      120

gacttcgcct gtgat                                                       135


<210>  16
<211>  74
<212>  DNA
<213>  Homo sapiens

<400>  16
atctacatct gggcgccctt ggccgggact tgtggggtcc ttctcctgtc actggttatc       60

accctttact gcaa                                                         74


