                               SEQUENCE LISTING

<110> PFENEX INC.

<120> METHOD FOR PRODUCTION OF RECOMBINANT E. COLI ASPARAGINASE

<130> 38194-751.601

<140>
<141>

<150> 62/578,302
<151> 2017-10-27

<160> 57    

<170> PatentIn version 3.5

<210> 1
<211> 326
<212> PRT
<213> Escherichia coli

<400> 1
Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Ala Gly Lys Val Gly Val 
            20                  25                  30          


Glu Asn Leu Val Asn Ala Val Pro Gln Leu Lys Asp Ile Ala Asn Val 
        35                  40                  45              


Lys Gly Glu Gln Val Val Asn Ile Gly Ser Gln Asp Met Asn Asp Asp 
    50                  55                  60                  


Val Trp Leu Thr Leu Ala Lys Lys Ile Asn Thr Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Lys Ala Ser Ala Asn Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Lys His Thr Ser Asp Thr Pro Phe Asp Val Ser Lys Leu 
        195                 200                 205             


Asn Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Asp Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Thr Val Phe Asp Thr 
                245                 250                 255     


Leu Ala Thr Ala Ala Lys Asn Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Gln Thr Lys Asp Pro Gln Gln Ile Gln 
305                 310                 315                 320 


Gln Ile Phe Asn Gln Tyr 
                325     


<210> 2
<211> 978
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 2
ctccctaaca ttactattct ggccactggc ggtacgattg caggcggcgg tgactcagcc       60

accaagtcga attacaccgc cggtaaggtc ggtgtcgaaa acctcgtcaa cgccgtgccg      120

cagctgaaag atatcgccaa cgtcaagggc gagcaagtgg tgaacatcgg ctcccaagat      180

atgaacgatg acgtgtggct gacgctggcc aagaaaatca acaccgattg cgacaagacg      240

gacgggtttg tcatcaccca cggcaccgac actatggaag agactgccta cttcctcgac      300

ctcacggtga agtgcgataa accggtagtg atggtgggcg ccatgcgccc gagcacctcg      360

atgagcgcgg acggcccgtt caatctgtac aacgccgtgg taaccgcagc ggacaaggcg      420

tccgcgaacc gcggtgtatt ggtagtgatg aacgatacgg tgctcgatgg gcgcgatgtg      480

accaagacca ataccactga tgtggccacc ttcaagagcg tgaactatgg cccgctgggc      540

tacatccata acggcaagat cgattaccag cgtactcccg cccggaagca cacctcggac      600

acccccttcg acgtgtcgaa actgaacgaa ctgcccaagg tcggcatcgt ctacaactac      660

gccaatgcga gcgatctgcc cgcgaaggcc ctggtggacg ccggctacga cgggatcgta      720

tcggcgggtg tgggcaatgg taacctgtac aagaccgtgt ttgacaccct ggcgacggcg      780

gcgaagaacg gcaccgccgt ggtccgcagc agccgcgtgc ccactggggc gaccacccaa      840

gacgccgagg tcgacgacgc gaagtacggc ttcgtagcca gcggcaccct gaacccgcaa      900

aaggcccggg tcctgctgca gctggcgctc acgcagacga aggacccgca gcaaatccaa      960

cagatcttca accagtac                                                    978


<210> 3
<211> 348
<212> PRT
<213> Escherichia coli

<400> 3
Met Glu Phe Phe Lys Lys Thr Ala Leu Ala Ala Leu Val Met Gly Phe 
1               5                   10                  15      


Ser Gly Ala Ala Leu Ala Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly 
            20                  25                  30          


Gly Thr Ile Ala Gly Gly Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr 
        35                  40                  45              


Val Gly Lys Val Gly Val Glu Asn Leu Val Asn Ala Val Pro Gln Leu 
    50                  55                  60                  


Lys Asp Ile Ala Asn Val Lys Gly Glu Gln Val Val Asn Ile Gly Ser 
65                  70                  75                  80  


Gln Asp Met Asn Asp Asn Val Trp Leu Thr Leu Ala Lys Lys Ile Asn 
                85                  90                  95      


Thr Asp Cys Asp Lys Thr Asp Gly Phe Val Ile Thr His Gly Thr Asp 
            100                 105                 110         


Thr Met Glu Glu Thr Ala Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp 
        115                 120                 125             


Lys Pro Val Val Met Val Gly Ala Met Arg Pro Ser Thr Ser Met Ser 
    130                 135                 140                 


Ala Asp Gly Pro Phe Asn Leu Tyr Asn Ala Val Val Thr Ala Ala Asp 
145                 150                 155                 160 


Lys Ala Ser Ala Asn Arg Gly Val Leu Val Val Met Asn Asp Thr Val 
                165                 170                 175     


Leu Asp Gly Arg Asp Val Thr Lys Thr Asn Thr Thr Asp Val Ala Thr 
            180                 185                 190         


Phe Lys Ser Val Asn Tyr Gly Pro Leu Gly Tyr Ile His Asn Gly Lys 
        195                 200                 205             


Ile Asp Tyr Gln Arg Thr Pro Ala Arg Lys His Thr Ser Asp Thr Pro 
    210                 215                 220                 


Phe Asp Val Ser Lys Leu Asn Glu Leu Pro Lys Val Gly Ile Val Tyr 
225                 230                 235                 240 


Asn Tyr Ala Asn Ala Ser Asp Leu Pro Ala Lys Ala Leu Val Asp Ala 
                245                 250                 255     


Gly Tyr Asp Gly Ile Val Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr 
            260                 265                 270         


Lys Ser Val Phe Asp Thr Leu Ala Thr Ala Ala Lys Thr Gly Thr Ala 
        275                 280                 285             


Val Val Arg Ser Ser Arg Val Pro Thr Gly Ala Thr Thr Gln Asp Ala 
    290                 295                 300                 


Glu Val Asp Asp Ala Lys Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn 
305                 310                 315                 320 


Pro Gln Lys Ala Arg Val Leu Leu Gln Leu Ala Leu Thr Gln Thr Lys 
                325                 330                 335     


Asp Pro Gln Gln Ile Gln Gln Ile Phe Asn Gln Tyr 
            340                 345             


<210> 4
<211> 1047
<212> DNA
<213> Escherichia coli

<400> 4
atggagtttt tcaaaaagac ggcacttgcc gcactggtta tgggttttag tggtgcagca       60

ttggcattac ccaatatcac cattttagca accggcggga ccattgccgg tggtggtgac      120

tccgcaacca aatctaacta cacagtgggt aaagttggcg tagaaaatct ggttaatgcg      180

gtgccgcaac taaaagacat tgcgaacgtt aaaggcgagc aggtagtgaa tatcggctcc      240

caggacatga acgataatgt ctggctgaca ctggcgaaaa aaattaacac cgactgcgat      300

aagaccgacg gcttcgtcat tacccacggt accgacacga tggaagaaac tgcttacttc      360

ctcgacctga cggtgaaatg cgacaaaccg gtggtgatgg tcggcgcaat gcgtccgtcc      420

acgtctatga gcgcagacgg tccattcaac ctgtataacg cggtagtgac cgcagctgat      480

aaagcctccg ccaaccgtgg cgtgctggta gtgatgaatg acaccgtgct tgatggccgt      540

gacgtcacca aaaccaacac caccgacgta gcgaccttca agtctgttaa ctacggtcct      600

ctgggttaca ttcacaacgg taagattgac taccagcgta ccccggcacg taagcatacc      660

agcgacacgc cattcgatgt ctctaagctg aatgaactgc cgaaagtcgg cattgtttat      720

aactacgcta acgcatccga tcttccggct aaagcactgg tagatgcggg ctatgatggc      780

atcgttagcg ctggtgtggg taacggcaac ctgtataaat ctgtgttcga cacgctggcg      840

accgccgcga aaaccggtac tgcagtcgtg cgttcttccc gcgtaccgac gggcgctacc      900

actcaggatg ccgaagtgga tgatgcgaaa tacggcttcg tcgcctctgg cacgctgaac      960

ccgcaaaaag cgcgcgttct gctgcaactg gctctgacgc aaaccaaaga tccgcagcag     1020

atccagcaga tcttcaatca gtactaa                                         1047


<210> 5
<211> 326
<212> PRT
<213> Escherichia coli

<400> 5
Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Val Gly Lys Val Gly Val 
            20                  25                  30          


Glu Asn Leu Val Asn Ala Val Pro Gln Leu Lys Asp Ile Ala Asn Val 
        35                  40                  45              


Lys Gly Glu Gln Val Val Asn Ile Gly Ser Gln Asp Met Asn Asp Asn 
    50                  55                  60                  


Val Trp Leu Thr Leu Ala Lys Lys Ile Asn Thr Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Lys Ala Ser Ala Asn Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Lys His Thr Ser Asp Thr Pro Phe Asp Val Ser Lys Leu 
        195                 200                 205             


Asn Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Asp Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Ser Val Phe Asp Thr 
                245                 250                 255     


Leu Ala Thr Ala Ala Lys Thr Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Gln Thr Lys Asp Pro Gln Gln Ile Gln 
305                 310                 315                 320 


Gln Ile Phe Asn Gln Tyr 
                325     


<210> 6
<211> 351
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 6
Met Lys Ser Ala Leu Lys Asn Val Ile Pro Gly Ala Leu Ala Leu Leu 
1               5                   10                  15      


Leu Leu Phe Pro Val Ala Ala Gln Ala Leu Pro Asn Ile Thr Ile Leu 
            20                  25                  30          


Ala Thr Gly Gly Thr Ile Ala Gly Gly Gly Asp Ser Ala Thr Lys Ser 
        35                  40                  45              


Asn Tyr Thr Ala Gly Lys Val Gly Val Glu Asn Leu Val Asn Ala Val 
    50                  55                  60                  


Pro Gln Leu Lys Asp Ile Ala Asn Val Lys Gly Glu Gln Val Val Asn 
65                  70                  75                  80  


Ile Gly Ser Gln Asp Met Asn Asp Asp Val Trp Leu Thr Leu Ala Lys 
                85                  90                  95      


Lys Ile Asn Thr Asp Cys Asp Lys Thr Asp Gly Phe Val Ile Thr His 
            100                 105                 110         


Gly Thr Asp Thr Met Glu Glu Thr Ala Tyr Phe Leu Asp Leu Thr Val 
        115                 120                 125             


Lys Cys Asp Lys Pro Val Val Met Val Gly Ala Met Arg Pro Ser Thr 
    130                 135                 140                 


Ser Met Ser Ala Asp Gly Pro Phe Asn Leu Tyr Asn Ala Val Val Thr 
145                 150                 155                 160 


Ala Ala Asp Lys Ala Ser Ala Asn Arg Gly Val Leu Val Val Met Asn 
                165                 170                 175     


Asp Thr Val Leu Asp Gly Arg Asp Val Thr Lys Thr Asn Thr Thr Asp 
            180                 185                 190         


Val Ala Thr Phe Lys Ser Val Asn Tyr Gly Pro Leu Gly Tyr Ile His 
        195                 200                 205             


Asn Gly Lys Ile Asp Tyr Gln Arg Thr Pro Ala Arg Lys His Thr Ser 
    210                 215                 220                 


Asp Thr Pro Phe Asp Val Ser Lys Leu Asn Glu Leu Pro Lys Val Gly 
225                 230                 235                 240 


Ile Val Tyr Asn Tyr Ala Asn Ala Ser Asp Leu Pro Ala Lys Ala Leu 
                245                 250                 255     


Val Asp Ala Gly Tyr Asp Gly Ile Val Ser Ala Gly Val Gly Asn Gly 
            260                 265                 270         


Asn Leu Tyr Lys Thr Val Phe Asp Thr Leu Ala Thr Ala Ala Lys Asn 
        275                 280                 285             


Gly Thr Ala Val Val Arg Ser Ser Arg Val Pro Thr Gly Ala Thr Thr 
    290                 295                 300                 


Gln Asp Ala Glu Val Asp Asp Ala Lys Tyr Gly Phe Val Ala Ser Gly 
305                 310                 315                 320 


Thr Leu Asn Pro Gln Lys Ala Arg Val Leu Leu Gln Leu Ala Leu Thr 
                325                 330                 335     


Gln Thr Lys Asp Pro Gln Gln Ile Gln Gln Ile Phe Asn Gln Tyr 
            340                 345                 350     


<210> 7
<211> 344
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 7
Met Arg Gln Leu Phe Phe Cys Leu Met Leu Met Val Ser Leu Thr Ala 
1               5                   10                  15      


His Ala Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala 
            20                  25                  30          


Gly Gly Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Ala Gly Lys Val 
        35                  40                  45              


Gly Val Glu Asn Leu Val Asn Ala Val Pro Gln Leu Lys Asp Ile Ala 
    50                  55                  60                  


Asn Val Lys Gly Glu Gln Val Val Asn Ile Gly Ser Gln Asp Met Asn 
65                  70                  75                  80  


Asp Asp Val Trp Leu Thr Leu Ala Lys Lys Ile Asn Thr Asp Cys Asp 
                85                  90                  95      


Lys Thr Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu 
            100                 105                 110         


Thr Ala Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val 
        115                 120                 125             


Met Val Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro 
    130                 135                 140                 


Phe Asn Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Lys Ala Ser Ala 
145                 150                 155                 160 


Asn Arg Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg 
                165                 170                 175     


Asp Val Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val 
            180                 185                 190         


Asn Tyr Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln 
        195                 200                 205             


Arg Thr Pro Ala Arg Lys His Thr Ser Asp Thr Pro Phe Asp Val Ser 
    210                 215                 220                 


Lys Leu Asn Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn 
225                 230                 235                 240 


Ala Ser Asp Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly 
                245                 250                 255     


Ile Val Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Thr Val Phe 
            260                 265                 270         


Asp Thr Leu Ala Thr Ala Ala Lys Asn Gly Thr Ala Val Val Arg Ser 
        275                 280                 285             


Ser Arg Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp 
    290                 295                 300                 


Ala Lys Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala 
305                 310                 315                 320 


Arg Val Leu Leu Gln Leu Ala Leu Thr Gln Thr Lys Asp Pro Gln Gln 
                325                 330                 335     


Ile Gln Gln Ile Phe Asn Gln Tyr 
            340                 


<210> 8
<211> 357
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 8
Met Ile Arg Asp Asn Arg Leu Lys Thr Ser Leu Leu Arg Gly Leu Thr 
1               5                   10                  15      


Leu Thr Leu Leu Ser Leu Thr Leu Leu Ser Pro Ala Ala His Ala Leu 
            20                  25                  30          


Pro Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly Gly 
        35                  40                  45              


Asp Ser Ala Thr Lys Ser Asn Tyr Thr Ala Gly Lys Val Gly Val Glu 
    50                  55                  60                  


Asn Leu Val Asn Ala Val Pro Gln Leu Lys Asp Ile Ala Asn Val Lys 
65                  70                  75                  80  


Gly Glu Gln Val Val Asn Ile Gly Ser Gln Asp Met Asn Asp Asp Val 
                85                  90                  95      


Trp Leu Thr Leu Ala Lys Lys Ile Asn Thr Asp Cys Asp Lys Thr Asp 
            100                 105                 110         


Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala Tyr 
        115                 120                 125             


Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val Gly 
    130                 135                 140                 


Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn Leu 
145                 150                 155                 160 


Tyr Asn Ala Val Val Thr Ala Ala Asp Lys Ala Ser Ala Asn Arg Gly 
                165                 170                 175     


Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val Thr 
            180                 185                 190         


Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr Gly 
        195                 200                 205             


Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr Pro 
    210                 215                 220                 


Ala Arg Lys His Thr Ser Asp Thr Pro Phe Asp Val Ser Lys Leu Asn 
225                 230                 235                 240 


Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser Asp 
                245                 250                 255     


Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val Ser 
            260                 265                 270         


Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Thr Val Phe Asp Thr Leu 
        275                 280                 285             


Ala Thr Ala Ala Lys Asn Gly Thr Ala Val Val Arg Ser Ser Arg Val 
    290                 295                 300                 


Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys Tyr 
305                 310                 315                 320 


Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val Leu 
                325                 330                 335     


Leu Gln Leu Ala Leu Thr Gln Thr Lys Asp Pro Gln Gln Ile Gln Gln 
            340                 345                 350         


Ile Phe Asn Gln Tyr 
        355         


<210> 9
<211> 350
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 9
Met Lys Leu Lys Arg Leu Met Ala Ala Met Thr Phe Val Ala Ala Gly 
1               5                   10                  15      


Val Ala Thr Ala Asn Ala Val Ala Leu Pro Asn Ile Thr Ile Leu Ala 
            20                  25                  30          


Thr Gly Gly Thr Ile Ala Gly Gly Gly Asp Ser Ala Thr Lys Ser Asn 
        35                  40                  45              


Tyr Thr Ala Gly Lys Val Gly Val Glu Asn Leu Val Asn Ala Val Pro 
    50                  55                  60                  


Gln Leu Lys Asp Ile Ala Asn Val Lys Gly Glu Gln Val Val Asn Ile 
65                  70                  75                  80  


Gly Ser Gln Asp Met Asn Asp Asp Val Trp Leu Thr Leu Ala Lys Lys 
                85                  90                  95      


Ile Asn Thr Asp Cys Asp Lys Thr Asp Gly Phe Val Ile Thr His Gly 
            100                 105                 110         


Thr Asp Thr Met Glu Glu Thr Ala Tyr Phe Leu Asp Leu Thr Val Lys 
        115                 120                 125             


Cys Asp Lys Pro Val Val Met Val Gly Ala Met Arg Pro Ser Thr Ser 
    130                 135                 140                 


Met Ser Ala Asp Gly Pro Phe Asn Leu Tyr Asn Ala Val Val Thr Ala 
145                 150                 155                 160 


Ala Asp Lys Ala Ser Ala Asn Arg Gly Val Leu Val Val Met Asn Asp 
                165                 170                 175     


Thr Val Leu Asp Gly Arg Asp Val Thr Lys Thr Asn Thr Thr Asp Val 
            180                 185                 190         


Ala Thr Phe Lys Ser Val Asn Tyr Gly Pro Leu Gly Tyr Ile His Asn 
        195                 200                 205             


Gly Lys Ile Asp Tyr Gln Arg Thr Pro Ala Arg Lys His Thr Ser Asp 
    210                 215                 220                 


Thr Pro Phe Asp Val Ser Lys Leu Asn Glu Leu Pro Lys Val Gly Ile 
225                 230                 235                 240 


Val Tyr Asn Tyr Ala Asn Ala Ser Asp Leu Pro Ala Lys Ala Leu Val 
                245                 250                 255     


Asp Ala Gly Tyr Asp Gly Ile Val Ser Ala Gly Val Gly Asn Gly Asn 
            260                 265                 270         


Leu Tyr Lys Thr Val Phe Asp Thr Leu Ala Thr Ala Ala Lys Asn Gly 
        275                 280                 285             


Thr Ala Val Val Arg Ser Ser Arg Val Pro Thr Gly Ala Thr Thr Gln 
    290                 295                 300                 


Asp Ala Glu Val Asp Asp Ala Lys Tyr Gly Phe Val Ala Ser Gly Thr 
305                 310                 315                 320 


Leu Asn Pro Gln Lys Ala Arg Val Leu Leu Gln Leu Ala Leu Thr Gln 
                325                 330                 335     


Thr Lys Asp Pro Gln Gln Ile Gln Gln Ile Phe Asn Gln Tyr 
            340                 345                 350 


<210> 10
<211> 349
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 10
Met Gln Asn Tyr Lys Lys Phe Leu Leu Ala Ala Ala Val Ser Met Ala 
1               5                   10                  15      


Phe Ser Ala Thr Ala Met Ala Leu Pro Asn Ile Thr Ile Leu Ala Thr 
            20                  25                  30          


Gly Gly Thr Ile Ala Gly Gly Gly Asp Ser Ala Thr Lys Ser Asn Tyr 
        35                  40                  45              


Thr Ala Gly Lys Val Gly Val Glu Asn Leu Val Asn Ala Val Pro Gln 
    50                  55                  60                  


Leu Lys Asp Ile Ala Asn Val Lys Gly Glu Gln Val Val Asn Ile Gly 
65                  70                  75                  80  


Ser Gln Asp Met Asn Asp Asp Val Trp Leu Thr Leu Ala Lys Lys Ile 
                85                  90                  95      


Asn Thr Asp Cys Asp Lys Thr Asp Gly Phe Val Ile Thr His Gly Thr 
            100                 105                 110         


Asp Thr Met Glu Glu Thr Ala Tyr Phe Leu Asp Leu Thr Val Lys Cys 
        115                 120                 125             


Asp Lys Pro Val Val Met Val Gly Ala Met Arg Pro Ser Thr Ser Met 
    130                 135                 140                 


Ser Ala Asp Gly Pro Phe Asn Leu Tyr Asn Ala Val Val Thr Ala Ala 
145                 150                 155                 160 


Asp Lys Ala Ser Ala Asn Arg Gly Val Leu Val Val Met Asn Asp Thr 
                165                 170                 175     


Val Leu Asp Gly Arg Asp Val Thr Lys Thr Asn Thr Thr Asp Val Ala 
            180                 185                 190         


Thr Phe Lys Ser Val Asn Tyr Gly Pro Leu Gly Tyr Ile His Asn Gly 
        195                 200                 205             


Lys Ile Asp Tyr Gln Arg Thr Pro Ala Arg Lys His Thr Ser Asp Thr 
    210                 215                 220                 


Pro Phe Asp Val Ser Lys Leu Asn Glu Leu Pro Lys Val Gly Ile Val 
225                 230                 235                 240 


Tyr Asn Tyr Ala Asn Ala Ser Asp Leu Pro Ala Lys Ala Leu Val Asp 
                245                 250                 255     


Ala Gly Tyr Asp Gly Ile Val Ser Ala Gly Val Gly Asn Gly Asn Leu 
            260                 265                 270         


Tyr Lys Thr Val Phe Asp Thr Leu Ala Thr Ala Ala Lys Asn Gly Thr 
        275                 280                 285             


Ala Val Val Arg Ser Ser Arg Val Pro Thr Gly Ala Thr Thr Gln Asp 
    290                 295                 300                 


Ala Glu Val Asp Asp Ala Lys Tyr Gly Phe Val Ala Ser Gly Thr Leu 
305                 310                 315                 320 


Asn Pro Gln Lys Ala Arg Val Leu Leu Gln Leu Ala Leu Thr Gln Thr 
                325                 330                 335     


Lys Asp Pro Gln Gln Ile Gln Gln Ile Phe Asn Gln Tyr 
            340                 345                 


<210> 11
<211> 346
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 11
Met Phe Ala Lys Leu Val Ala Val Ser Leu Leu Thr Leu Ala Ser Gly 
1               5                   10                  15      


Gln Leu Leu Ala Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr 
            20                  25                  30          


Ile Ala Gly Gly Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Ala Gly 
        35                  40                  45              


Lys Val Gly Val Glu Asn Leu Val Asn Ala Val Pro Gln Leu Lys Asp 
    50                  55                  60                  


Ile Ala Asn Val Lys Gly Glu Gln Val Val Asn Ile Gly Ser Gln Asp 
65                  70                  75                  80  


Met Asn Asp Asp Val Trp Leu Thr Leu Ala Lys Lys Ile Asn Thr Asp 
                85                  90                  95      


Cys Asp Lys Thr Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met 
            100                 105                 110         


Glu Glu Thr Ala Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro 
        115                 120                 125             


Val Val Met Val Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp 
    130                 135                 140                 


Gly Pro Phe Asn Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Lys Ala 
145                 150                 155                 160 


Ser Ala Asn Arg Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp 
                165                 170                 175     


Gly Arg Asp Val Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys 
            180                 185                 190         


Ser Val Asn Tyr Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp 
        195                 200                 205             


Tyr Gln Arg Thr Pro Ala Arg Lys His Thr Ser Asp Thr Pro Phe Asp 
    210                 215                 220                 


Val Ser Lys Leu Asn Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr 
225                 230                 235                 240 


Ala Asn Ala Ser Asp Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr 
                245                 250                 255     


Asp Gly Ile Val Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Thr 
            260                 265                 270         


Val Phe Asp Thr Leu Ala Thr Ala Ala Lys Asn Gly Thr Ala Val Val 
        275                 280                 285             


Arg Ser Ser Arg Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val 
    290                 295                 300                 


Asp Asp Ala Lys Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln 
305                 310                 315                 320 


Lys Ala Arg Val Leu Leu Gln Leu Ala Leu Thr Gln Thr Lys Asp Pro 
                325                 330                 335     


Gln Gln Ile Gln Gln Ile Phe Asn Gln Tyr 
            340                 345     


<210> 12
<211> 350
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 12
Met Lys Leu Lys Arg Leu Met Ala Ala Met Thr Phe Val Ala Ala Gly 
1               5                   10                  15      


Val Ala Thr Val Asn Ala Val Ala Leu Pro Asn Ile Thr Ile Leu Ala 
            20                  25                  30          


Thr Gly Gly Thr Ile Ala Gly Gly Gly Asp Ser Ala Thr Lys Ser Asn 
        35                  40                  45              


Tyr Thr Ala Gly Lys Val Gly Val Glu Asn Leu Val Asn Ala Val Pro 
    50                  55                  60                  


Gln Leu Lys Asp Ile Ala Asn Val Lys Gly Glu Gln Val Val Asn Ile 
65                  70                  75                  80  


Gly Ser Gln Asp Met Asn Asp Asp Val Trp Leu Thr Leu Ala Lys Lys 
                85                  90                  95      


Ile Asn Thr Asp Cys Asp Lys Thr Asp Gly Phe Val Ile Thr His Gly 
            100                 105                 110         


Thr Asp Thr Met Glu Glu Thr Ala Tyr Phe Leu Asp Leu Thr Val Lys 
        115                 120                 125             


Cys Asp Lys Pro Val Val Met Val Gly Ala Met Arg Pro Ser Thr Ser 
    130                 135                 140                 


Met Ser Ala Asp Gly Pro Phe Asn Leu Tyr Asn Ala Val Val Thr Ala 
145                 150                 155                 160 


Ala Asp Lys Ala Ser Ala Asn Arg Gly Val Leu Val Val Met Asn Asp 
                165                 170                 175     


Thr Val Leu Asp Gly Arg Asp Val Thr Lys Thr Asn Thr Thr Asp Val 
            180                 185                 190         


Ala Thr Phe Lys Ser Val Asn Tyr Gly Pro Leu Gly Tyr Ile His Asn 
        195                 200                 205             


Gly Lys Ile Asp Tyr Gln Arg Thr Pro Ala Arg Lys His Thr Ser Asp 
    210                 215                 220                 


Thr Pro Phe Asp Val Ser Lys Leu Asn Glu Leu Pro Lys Val Gly Ile 
225                 230                 235                 240 


Val Tyr Asn Tyr Ala Asn Ala Ser Asp Leu Pro Ala Lys Ala Leu Val 
                245                 250                 255     


Asp Ala Gly Tyr Asp Gly Ile Val Ser Ala Gly Val Gly Asn Gly Asn 
            260                 265                 270         


Leu Tyr Lys Thr Val Phe Asp Thr Leu Ala Thr Ala Ala Lys Asn Gly 
        275                 280                 285             


Thr Ala Val Val Arg Ser Ser Arg Val Pro Thr Gly Ala Thr Thr Gln 
    290                 295                 300                 


Asp Ala Glu Val Asp Asp Ala Lys Tyr Gly Phe Val Ala Ser Gly Thr 
305                 310                 315                 320 


Leu Asn Pro Gln Lys Ala Arg Val Leu Leu Gln Leu Ala Leu Thr Gln 
                325                 330                 335     


Thr Lys Asp Pro Gln Gln Ile Gln Gln Ile Phe Asn Gln Tyr 
            340                 345                 350 


<210> 13
<211> 349
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<400> 13
Met Pro Pro Arg Ser Ile Ala Ala Cys Leu Gly Leu Leu Gly Leu Leu 
1               5                   10                  15      


Met Ala Thr Gln Ala Ala Ala Leu Pro Asn Ile Thr Ile Leu Ala Thr 
            20                  25                  30          


Gly Gly Thr Ile Ala Gly Gly Gly Asp Ser Ala Thr Lys Ser Asn Tyr 
        35                  40                  45              


Thr Ala Gly Lys Val Gly Val Glu Asn Leu Val Asn Ala Val Pro Gln 
    50                  55                  60                  


Leu Lys Asp Ile Ala Asn Val Lys Gly Glu Gln Val Val Asn Ile Gly 
65                  70                  75                  80  


Ser Gln Asp Met Asn Asp Asp Val Trp Leu Thr Leu Ala Lys Lys Ile 
                85                  90                  95      


Asn Thr Asp Cys Asp Lys Thr Asp Gly Phe Val Ile Thr His Gly Thr 
            100                 105                 110         


Asp Thr Met Glu Glu Thr Ala Tyr Phe Leu Asp Leu Thr Val Lys Cys 
        115                 120                 125             


Asp Lys Pro Val Val Met Val Gly Ala Met Arg Pro Ser Thr Ser Met 
    130                 135                 140                 


Ser Ala Asp Gly Pro Phe Asn Leu Tyr Asn Ala Val Val Thr Ala Ala 
145                 150                 155                 160 


Asp Lys Ala Ser Ala Asn Arg Gly Val Leu Val Val Met Asn Asp Thr 
                165                 170                 175     


Val Leu Asp Gly Arg Asp Val Thr Lys Thr Asn Thr Thr Asp Val Ala 
            180                 185                 190         


Thr Phe Lys Ser Val Asn Tyr Gly Pro Leu Gly Tyr Ile His Asn Gly 
        195                 200                 205             


Lys Ile Asp Tyr Gln Arg Thr Pro Ala Arg Lys His Thr Ser Asp Thr 
    210                 215                 220                 


Pro Phe Asp Val Ser Lys Leu Asn Glu Leu Pro Lys Val Gly Ile Val 
225                 230                 235                 240 


Tyr Asn Tyr Ala Asn Ala Ser Asp Leu Pro Ala Lys Ala Leu Val Asp 
                245                 250                 255     


Ala Gly Tyr Asp Gly Ile Val Ser Ala Gly Val Gly Asn Gly Asn Leu 
            260                 265                 270         


Tyr Lys Thr Val Phe Asp Thr Leu Ala Thr Ala Ala Lys Asn Gly Thr 
        275                 280                 285             


Ala Val Val Arg Ser Ser Arg Val Pro Thr Gly Ala Thr Thr Gln Asp 
    290                 295                 300                 


Ala Glu Val Asp Asp Ala Lys Tyr Gly Phe Val Ala Ser Gly Thr Leu 
305                 310                 315                 320 


Asn Pro Gln Lys Ala Arg Val Leu Leu Gln Leu Ala Leu Thr Gln Thr 
                325                 330                 335     


Lys Asp Pro Gln Gln Ile Gln Gln Ile Phe Asn Gln Tyr 
            340                 345                 


<210> 14
<211> 25
<212> PRT
<213> Pseudomonas fluorescens

<400> 14
Met Lys Ser Ala Leu Lys Asn Val Ile Pro Gly Ala Leu Ala Leu Leu 
1               5                   10                  15      


Leu Leu Phe Pro Val Ala Ala Gln Ala 
            20                  25  


<210> 15
<211> 75
<212> DNA
<213> Pseudomonas fluorescens

<400> 15
atgaaatctg cattgaagaa cgttattccg ggcgccctgg cccttctgct gctattcccc       60

gtcgccgccc aggca                                                        75


<210> 16
<211> 18
<212> PRT
<213> Pseudomonas fluorescens

<400> 16
Met Arg Gln Leu Phe Phe Cys Leu Met Leu Met Val Ser Leu Thr Ala 
1               5                   10                  15      


His Ala 
        


<210> 17
<211> 54
<212> DNA
<213> Pseudomonas fluorescens

<400> 17
atgcgacaac tatttttctg tttgatgctg atggtgtcgc tcacggcgca cgcc             54


<210> 18
<211> 31
<212> PRT
<213> Pseudomonas fluorescens

<400> 18
Met Ile Arg Asp Asn Arg Leu Lys Thr Ser Leu Leu Arg Gly Leu Thr 
1               5                   10                  15      


Leu Thr Leu Leu Ser Leu Thr Leu Leu Ser Pro Ala Ala His Ala 
            20                  25                  30      


<210> 19
<211> 93
<212> DNA
<213> Pseudomonas fluorescens

<400> 19
atgatccgtg acaaccgact caagacatcc cttctgcgcg gcctgaccct caccctactc       60

agcctgaccc tgctctcgcc cgcggcccat gcc                                    93


<210> 20
<211> 24
<212> PRT
<213> Pseudomonas fluorescens

<400> 20
Met Lys Leu Lys Arg Leu Met Ala Ala Met Thr Phe Val Ala Ala Gly 
1               5                   10                  15      


Val Ala Thr Ala Asn Ala Val Ala 
            20                  


<210> 21
<211> 72
<212> DNA
<213> Pseudomonas fluorescens

<400> 21
atgaaactga aacgtttgat ggcggcaatg acttttgtcg ctgctggcgt tgcgaccgcc       60

aacgcggtgg cc                                                           72


<210> 22
<211> 23
<212> PRT
<213> Pseudomonas fluorescens

<400> 22
Met Gln Asn Tyr Lys Lys Phe Leu Leu Ala Ala Ala Val Ser Met Ala 
1               5                   10                  15      


Phe Ser Ala Thr Ala Met Ala 
            20              


<210> 23
<211> 69
<212> DNA
<213> Pseudomonas fluorescens

<400> 23
atgcagaact ataaaaaatt ccttctggcc gcggccgtct cgatggcgtt cagcgccacg       60

gccatggca                                                               69


<210> 24
<211> 20
<212> PRT
<213> Pseudomonas fluorescens

<400> 24
Met Phe Ala Lys Leu Val Ala Val Ser Leu Leu Thr Leu Ala Ser Gly 
1               5                   10                  15      


Gln Leu Leu Ala 
            20  


<210> 25
<211> 60
<212> DNA
<213> Pseudomonas fluorescens

<400> 25
atgtttgcca aactcgttgc tgtttccctg ctgactctgg cgagcggcca gttgcttgct       60


<210> 26
<211> 24
<212> PRT
<213> Pseudomonas fluorescens

<400> 26
Met Lys Leu Lys Arg Leu Met Ala Ala Met Thr Phe Val Ala Ala Gly 
1               5                   10                  15      


Val Ala Thr Val Asn Ala Val Ala 
            20                  


<210> 27
<211> 72
<212> DNA
<213> Pseudomonas fluorescens

<400> 27
atgaaactga aacgtttgat ggcggcaatg acttttgtcg ctgctggcgt tgcgaccgtc       60

aacgcggtgg cc                                                           72


<210> 28
<211> 23
<212> PRT
<213> Pseudomonas fluorescens

<400> 28
Met Pro Pro Arg Ser Ile Ala Ala Cys Leu Gly Leu Leu Gly Leu Leu 
1               5                   10                  15      


Met Ala Thr Gln Ala Ala Ala 
            20              


<210> 29
<211> 69
<212> DNA
<213> Pseudomonas fluorescens

<400> 29
atgccgcctc gttctatcgc cgcatgtctg gggctgctgg gcttgctcat ggctacccag       60

gccgccgcc                                                               69


<210> 30
<211> 22
<212> PRT
<213> Escherichia coli

<400> 30
Met Glu Phe Phe Lys Lys Thr Ala Leu Ala Ala Leu Val Met Gly Phe 
1               5                   10                  15      


Ser Gly Ala Ala Leu Ala 
            20          


<210> 31
<211> 33
<212> PRT
<213> Pseudomonas fluorescens

<400> 31
Met Gln Asn Arg Thr Val Glu Ile Gly Val Gly Leu Phe Leu Leu Ala 
1               5                   10                  15      


Gly Ile Leu Ala Leu Leu Leu Leu Ala Leu Arg Val Ser Gly Leu Ser 
            20                  25                  30          


Ala 
    


<210> 32
<211> 99
<212> DNA
<213> Pseudomonas fluorescens

<400> 32
atgcaaaacc gcactgtgga aatcggtgtc ggccttttct tgctggctgg catcctggct       60

ttactgttgt tggccctgcg agtcagcggc ctttcggcc                              99


<210> 33
<211> 21
<212> PRT
<213> Pseudomonas fluorescens

<400> 33
Met Asn Arg Ser Ser Ala Leu Leu Leu Ala Phe Val Phe Leu Ser Gly 
1               5                   10                  15      


Cys Gln Ala Met Ala 
            20      


<210> 34
<211> 63
<212> DNA
<213> Pseudomonas fluorescens

<400> 34
atgaatagat cttccgcgtt gctcctcgct tttgtcttcc tcagcggctg ccaggccatg       60

gcc                                                                     63


<210> 35
<211> 21
<212> PRT
<213> Pseudomonas fluorescens

<400> 35
Met Arg Leu Thr Gln Ile Ile Ala Ala Ala Ala Ile Ala Leu Val Ser 
1               5                   10                  15      


Thr Phe Ala Leu Ala 
            20      


<210> 36
<211> 63
<212> DNA
<213> Pseudomonas fluorescens

<400> 36
atgcgcttga cccagattat tgccgccgca gccattgcgt tggtttccac ctttgcgctc       60

gcc                                                                     63


<210> 37
<211> 29
<212> PRT
<213> Pseudomonas fluorescens

<400> 37
Met Gln Ser Leu Pro Phe Ser Ala Leu Arg Leu Leu Gly Val Leu Ala 
1               5                   10                  15      


Val Met Val Cys Val Leu Leu Thr Thr Pro Ala Arg Ala 
            20                  25                  


<210> 38
<211> 87
<212> DNA
<213> Pseudomonas fluorescens

<400> 38
atgcaaagcc tgccgttctc tgcgttacgc ctgctcggtg tgctggcagt catggtctgc       60

gtgctgttga cgacgccagc ccgtgcc                                           87


<210> 39
<211> 22
<212> PRT
<213> Pseudomonas fluorescens

<400> 39
Met Arg Asn Leu Ile Leu Ser Ala Ala Leu Val Thr Ala Ser Leu Phe 
1               5                   10                  15      


Gly Met Thr Ala Gln Ala 
            20          


<210> 40
<211> 66
<212> DNA
<213> Pseudomonas fluorescens

<400> 40
atgcgtaatc tgatcctcag cgccgctctc gtcactgcca gcctcttcgg catgaccgca       60

caagct                                                                  66


<210> 41
<211> 21
<212> PRT
<213> Pseudomonas fluorescens

<400> 41
Met Arg Asn Leu Leu Arg Gly Met Leu Val Val Ile Cys Cys Met Ala 
1               5                   10                  15      


Gly Ile Ala Ala Ala 
            20      


<210> 42
<211> 63
<212> DNA
<213> Pseudomonas fluorescens

<400> 42
atgagaaacc ttcttcgagg aatgcttgtc gttatttgct gtatggcagg gatagcggcg       60

gcg                                                                     63


<210> 43
<211> 24
<212> PRT
<213> Pseudomonas fluorescens

<400> 43
Met Leu Phe Arg Thr Leu Leu Ala Ser Leu Thr Phe Ala Val Ile Ala 
1               5                   10                  15      


Gly Leu Pro Ser Thr Ala His Ala 
            20                  


<210> 44
<211> 72
<212> DNA
<213> Pseudomonas fluorescens

<400> 44
atgctttttc gcacattact ggcgagcctt acctttgctg tcatcgccgg cttaccgtcc       60

acggcccacg cg                                                           72


<210> 45
<211> 6
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      consensus RBS sequence

<400> 45
aggagg                                                                   6


<210> 46
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS2 sequence

<400> 46
ggagcg                                                                   6


<210> 47
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS34 sequence

<400> 47
ggagcg                                                                   6


<210> 48
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS41 sequence

<400> 48
aggagt                                                                   6


<210> 49
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS43 sequence

<400> 49
ggagtg                                                                   6


<210> 50
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS48 sequence

<400> 50
gagtaa                                                                   6


<210> 51
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS1 sequence

<400> 51
agagag                                                                   6


<210> 52
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS35 sequence

<400> 52
aaggca                                                                   6


<210> 53
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS49 sequence

<400> 53
ccgaac                                                                   6


<210> 54
<211> 325
<212> PRT
<213> Pseudomonas fluorescens

<400> 54
Met Gln Ser Ala Asn Asn Val Met Val Leu Tyr Thr Gly Gly Thr Ile 
1               5                   10                  15      


Gly Met Gln Ala Ser Ala Asn Gly Leu Ala Pro Ala Ser Gly Phe Glu 
            20                  25                  30          


Val Arg Met Arg Glu Gln Phe Ala Gly Ala Asp Leu Pro Ala Trp Arg 
        35                  40                  45              


Phe Gln Glu Met Ser Pro Leu Ile Asp Ser Ala Asn Met Asn Pro Ala 
    50                  55                  60                  


Tyr Trp Gln Arg Leu Arg Ser Ala Val Val Glu Ala Val Asp Ala Gly 
65                  70                  75                  80  


Cys Asp Ala Val Leu Ile Leu His Gly Thr Asp Thr Leu Ala Tyr Ser 
                85                  90                  95      


Ala Ala Ala Met Ser Phe Gln Leu Leu Gly Leu Pro Ala Pro Val Val 
            100                 105                 110         


Phe Thr Gly Ser Met Leu Pro Ala Gly Val Pro Asp Ser Asp Ala Trp 
        115                 120                 125             


Glu Asn Val Ser Gly Ala Leu Thr Ala Leu Gly Glu Gly Leu Lys Pro 
    130                 135                 140                 


Gly Val His Leu Tyr Phe His Gly Ala Leu Met Ala Pro Thr Arg Cys 
145                 150                 155                 160 


Ala Lys Ile Arg Ser Phe Gly Arg Asn Pro Phe Ala Ala Leu Gln Arg 
                165                 170                 175     


Asn Gly Gly Val Ala Leu Ala Asp Lys Leu Pro Ala Ala Leu Ala Tyr 
            180                 185                 190         


Arg Asn Asp Lys Ala Pro Ala Asn Val Gly Val Leu Pro Leu Val Pro 
        195                 200                 205             


Gly Ile Ala Ala Ala Gln Leu Asp Ala Leu Ile Asp Ser Gly Ile Gln 
    210                 215                 220                 


Ala Leu Val Leu Glu Cys Phe Gly Ser Gly Thr Gly Pro Ser Asp Asn 
225                 230                 235                 240 


Pro Ala Phe Leu Ala Ser Leu Lys Arg Ala Gln Asp Gln Glu Val Val 
                245                 250                 255     


Val Val Ala Ile Thr Gln Cys His Glu Gly Gly Val Glu Leu Asp Val 
            260                 265                 270         


Tyr Glu Ala Gly Ser Arg Leu Arg Ser Val Gly Val Leu Ser Gly Gly 
        275                 280                 285             


Gly Met Thr Arg Glu Ala Ala Phe Gly Lys Leu Asn Ala Leu Ile Gly 
    290                 295                 300                 


Ala Gly Leu Asp Ser Ala Glu Ile Arg Arg Leu Val Glu Leu Asp Leu 
305                 310                 315                 320 


Cys Gly Glu Leu Ser 
                325 


<210> 55
<211> 362
<212> PRT
<213> Pseudomonas fluorescens

<400> 55
Met Lys Ser Ala Leu Lys Asn Val Ile Pro Gly Ala Leu Ala Leu Leu 
1               5                   10                  15      


Leu Leu Phe Pro Val Ala Ala Gln Ala Lys Glu Val Glu Ser Lys Thr 
            20                  25                  30          


Lys Leu Ser Asn Val Val Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly 
        35                  40                  45              


Ala Gly Ala Ser Ala Ala Asn Ser Ala Thr Tyr Gln Ala Ala Lys Val 
    50                  55                  60                  


Gly Ile Glu Gln Leu Ile Ala Gly Val Pro Glu Leu Ser Gln Ile Ala 
65                  70                  75                  80  


Asn Val Arg Gly Glu Gln Val Met Gln Ile Ala Ser Glu Ser Ile Asn 
                85                  90                  95      


Asn Glu Asn Leu Leu Gln Leu Gly Arg Arg Val Ala Glu Leu Ala Asp 
            100                 105                 110         


Asn Lys Asp Val Asp Gly Ile Val Ile Thr His Gly Thr Asp Thr Leu 
        115                 120                 125             


Glu Glu Thr Ala Tyr Phe Leu Asn Leu Val Glu Lys Thr Asp Lys Pro 
    130                 135                 140                 


Ile Val Val Val Gly Ser Met Arg Pro Gly Thr Ala Met Ser Ala Asp 
145                 150                 155                 160 


Gly Met Leu Asn Leu Tyr Asn Ala Val Ala Val Ala Gly Ser Lys Glu 
                165                 170                 175     


Ala Arg Gly Lys Gly Val Leu Val Thr Met Asn Asp Glu Ile Gln Ser 
            180                 185                 190         


Gly Arg Asp Val Ser Lys Met Ile Asn Ile Lys Thr Glu Ala Phe Lys 
        195                 200                 205             


Ser Pro Trp Gly Pro Met Gly Met Val Val Glu Gly Lys Ser Tyr Trp 
    210                 215                 220                 


Phe Arg Leu Pro Ala Lys Arg His Thr Met Asp Ser Glu Phe Asp Ile 
225                 230                 235                 240 


Lys Thr Ile Lys Ser Leu Pro Asp Val Glu Ile Ala Tyr Gly Tyr Gly 
                245                 250                 255     


Asn Val Ser Asp Thr Ala Tyr Lys Ala Leu Ala Gln Ala Gly Ala Lys 
            260                 265                 270         


Ala Ile Ile His Ala Gly Thr Gly Asn Gly Ser Val Ser Ser Lys Val 
        275                 280                 285             


Val Pro Ala Leu Val Glu Leu Arg Lys Gln Gly Val Gln Ile Ile Arg 
    290                 295                 300                 


Ser Ser His Val Asn Ala Gly Gly Met Val Leu Arg Asn Ala Glu Gln 
305                 310                 315                 320 


Pro Asp Asp Lys Tyr Asp Trp Val Ala Ala Leu Asp Leu Asn Pro Gln 
                325                 330                 335     


Lys Ala Arg Ile Leu Ala Met Val Ala Leu Thr Lys Thr Gln Asp Ser 
            340                 345                 350         


Lys Glu Leu Gln Arg Ile Phe Trp Glu Tyr 
        355                 360         


<210> 56
<211> 167
<212> PRT
<213> Pseudomonas fluorescens

<400> 56
Val Arg Lys Leu Thr Gln Leu Val Leu Leu Ala Thr Val Leu Val Thr 
1               5                   10                  15      


Thr Pro Ala Phe Ala Glu Met Lys Ile Ala Val Leu Asn Tyr Gln Met 
            20                  25                  30          


Ala Leu Leu Glu Ser Asp Ala Ala Lys Arg Tyr Ala Val Asp Ala Glu 
        35                  40                  45              


Lys Lys Phe Gly Pro Gln Leu Thr Lys Leu Lys Thr Leu Glu Ser Ser 
    50                  55                  60                  


Ala Lys Gly Ile Gln Asp Arg Leu Val Ala Gly Gly Asp Lys Met Gln 
65                  70                  75                  80  


Gln Gly Glu Arg Glu Arg Leu Glu Leu Glu Phe Lys Gln Lys Ala Arg 
                85                  90                  95      


Asp Tyr Gln Phe Gln Ser Lys Glu Leu Asn Glu Ala Lys Ala Val Ala 
            100                 105                 110         


Asp Arg Glu Met Leu Lys Gln Leu Lys Pro Lys Leu Asp Ser Ala Val 
        115                 120                 125             


Glu Glu Val Ile Lys Lys Gly Ala Phe Asp Leu Val Phe Glu Arg Gly 
    130                 135                 140                 


Ala Val Ile Asp Val Lys Pro Gln Tyr Asp Ile Thr Arg Gln Val Ile 
145                 150                 155                 160 


Glu Arg Met Asn Gln Leu Lys 
                165         


<210> 57
<211> 504
<212> DNA
<213> Pseudomonas fluorescens

<400> 57
gtgcgtaagt tgactcaatt ggtcttgctg gccactgtgc tggtcaccac cccggccttc       60

gccgaaatga aaatcgccgt tctgaactat cagatggccc tgctggaatc cgatgcggcc      120

aagcgatacg ccgtggatgc cgagaagaag ttcggtccgc aactgaccaa gctcaagaca      180

ctggaaagca gcgccaaagg catccaggac cgcctggtag ccggtggcga caagatgcag      240

caaggcgagc gcgagcgtct ggagcttgaa ttcaagcaaa aggcccgtga ctaccagttc      300

caatccaagg agctgaacga agccaaggct gtggccgacc gcgaaatgct caagcagctc      360

aagcctaaat tggacagcgc tgtggaagaa gtcatcaaga agggtgcctt tgacctggtg      420

ttcgagcgtg gcgccgtgat cgacgtcaag cctcaatacg acatcacccg ccaggtgatc      480

gagcgcatga accagctgaa gtga                                             504


