                         SEQUENCE LISTING

<110>  GO Therapeutics, Inc.
 
<120>  ANTI-GLYCO-MUC1 ANTIBODIES AND THEIR USES

<130>  GOT-001WO

<160>  52    

<170>  PatentIn version 3.5

<210>  1
<211>  136
<212>  PRT
<213>  Mus musculus

<400>  1

Met Gly Trp Ser Gly Ile Phe Leu Phe Phe Leu Ser Val Thr Thr Gly 
1               5                   10                  15      


Val His Ser Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys 
            20                  25                  30          


Pro Gly Ala Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe 
        35                  40                  45              


Thr Asp His Ala Ile His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu 
    50                  55                  60                  


Glu Trp Ile Gly Tyr Phe Ser Pro Gly Asn Asp Asp Ile His Tyr Asn 
65                  70                  75                  80  


Glu Lys Phe Glu Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser 
                85                  90                  95      


Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val 
            100                 105                 110         


Tyr Phe Cys Lys Arg Ser Tyr Asp Lys Asp Phe Asp Cys Trp Gly Gln 
        115                 120                 125             


Gly Thr Thr Leu Thr Val Ser Ser 
    130                 135     


<210>  2
<211>  140
<212>  PRT
<213>  Mus musculus

<400>  2

Met Val Leu Ile Leu Leu Leu Leu Trp Val Ser Gly Thr Cys Gly Asp 
1               5                   10                  15      


Ile Val Met Ser Gln Ser Pro Ser Ser Leu Gly Val Ser Val Gly Glu 
            20                  25                  30          


Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Tyr Ser Thr 
        35                  40                  45              


Asn Gln Lys Asn Tyr Gln Ser Leu Leu Tyr Ser Thr Asn Gln Lys Asn 
    50                  55                  60                  


Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu 
65                  70                  75                  80  


Ile Tyr Trp Val Ser Asn Arg Lys Ser Gly Val Pro Asp Arg Phe Thr 
                85                  90                  95      


Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Lys 
            100                 105                 110         


Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln Tyr Tyr Arg Tyr Pro 
        115                 120                 125             


Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
    130                 135                 140 


<210>  3
<211>  117
<212>  PRT
<213>  Mus musculus

<400>  3

Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Ala Ile His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Phe Ser Pro Gly Asn Asp Asp Ile His Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Glu Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Lys Arg Ser Tyr Asp Lys Asp Phe Asp Cys Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Leu Thr Val Ser Ser 
        115         


<210>  4
<211>  125
<212>  PRT
<213>  Mus musculus

<400>  4

Asp Ile Val Met Ser Gln Ser Pro Ser Ser Leu Gly Val Ser Val Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Tyr Ser 
            20                  25                  30          


Thr Asn Gln Lys Asn Tyr Gln Ser Leu Leu Tyr Ser Thr Asn Gln Lys 
        35                  40                  45              


Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu 
    50                  55                  60                  


Leu Ile Tyr Trp Val Ser Asn Arg Lys Ser Gly Val Pro Asp Arg Phe 
65                  70                  75                  80  


Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Val 
                85                  90                  95      


Lys Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln Tyr Tyr Arg Tyr 
            100                 105                 110         


Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
        115                 120                 125 


<210>  5
<211>  8
<212>  PRT
<213>  Mus musculus

<400>  5

Gly Tyr Thr Phe Thr Asp His Ala 
1               5               


<210>  6
<211>  8
<212>  PRT
<213>  Mus musculus

<400>  6

Phe Ser Pro Gly Asn Asp Asp Ile 
1               5               


<210>  7
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  7

Lys Arg Ser Tyr Asp Lys Asp Phe Asp Cys 
1               5                   10  


<210>  8
<211>  12
<212>  PRT
<213>  Mus musculus

<400>  8

Gln Ser Leu Leu Tyr Ser Thr Asn Gln Lys Asn Tyr 
1               5                   10          


<210>  9
<211>  3
<212>  PRT
<213>  Mus musculus

<400>  9

Trp Val Ser 
1           


<210>  10
<211>  9
<212>  PRT
<213>  Mus musculus

<400>  10

Gln Gln Tyr Tyr Arg Tyr Pro Leu Thr 
1               5                   


<210>  11
<211>  408
<212>  DNA
<213>  Mus musculus

<400>  11
atgggatgga gcgggatctt tctcttcttc ctgtcagtaa ctacaggtgt ccactcccag       60

gttcagctgc agcagtctga cgcggagttg gtgaaacctg gggcttcagt gaagatatcc      120

tgcaaggctt ctggctacac tttcactgac catgctattc actgggtgaa gcagaggcct      180

gaacagggcc tggaatggat tggatatttt tctcccggaa atgatgacat tcactacaat      240

gagaagttcg agggcaaggc cacactgact gcagacaaat cctccagcac tgcctacatg      300

cagctcaaca gcctgacatc tgaagattct gcagtgtatt tctgtaaaag atcttacgac      360

aaggactttg actgctgggg ccaaggcacc actctcacag tctcctca                   408


<210>  12
<211>  384
<212>  DNA
<213>  Mus musculus

<400>  12
atggttctta tcttactgct gctatgggta tctggtacct gtggggacat tgtgatgtca       60

cagtctccat cctccctagg tgtgtcagtt ggagagaagg ttactatgag ctgcaagtcc      120

agtcagagcc ttttatacag taccaatcaa aagaactacc tggcctggta ccagcagaaa      180

ccagggcagt ctcctaagtt gctgatttac tgggtatcta ataggaaatc tggggtccct      240

gatcgcttca caggcagtgg atctgggaca gatttcactc tcaccatcag tagtgtgaag      300

gctgaagacc tggcagttta ttactgtcag caatattata ggtatccgct cacgttcggt      360

gctgggacca agctggagct gaaa                                             384


<210>  13
<211>  351
<212>  DNA
<213>  Mus musculus

<400>  13
caggttcagc tgcagcagtc tgacgcggag ttggtgaaac ctggggcttc agtgaagata       60

tcctgcaagg cttctggcta cactttcact gaccatgcta ttcactgggt gaagcagagg      120

cctgaacagg gcctggaatg gattggatat ttttctcccg gaaatgatga cattcactac      180

aatgagaagt tcgagggcaa ggccacactg actgcagaca aatcctccag cactgcctac      240

atgcagctca acagcctgac atctgaagat tctgcagtgt atttctgtaa aagatcttac      300

gacaaggact ttgactgctg gggccaaggc accactctca cagtctcctc a               351


<210>  14
<211>  339
<212>  DNA
<213>  Mus musculus

<400>  14
gacattgtga tgtcacagtc tccatcctcc ctaggtgtgt cagttggaga gaaggttact       60

atgagctgca agtccagtca gagcctttta tacagtacca atcaaaagaa ctacctggcc      120

tggtaccagc agaaaccagg gcagtctcct aagttgctga tttactgggt atctaatagg      180

aaatctgggg tccctgatcg cttcacaggc agtggatctg ggacagattt cactctcacc      240

atcagtagtg tgaaggctga agacctggca gtttattact gtcagcaata ttataggtat      300

ccgctcacgt tcggtgctgg gaccaagctg gagctgaaa                             339


<210>  15
<211>  25
<212>  PRT
<213>  Mus musculus

<400>  15

Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser 
            20                  25  


<210>  16
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  16

Ile His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile Gly 
1               5                   10                  15      


Tyr 
    


<210>  17
<211>  38
<212>  PRT
<213>  Mus musculus

<400>  17

His Tyr Asn Glu Lys Phe Glu Gly Lys Ala Thr Leu Thr Ala Asp Lys 
1               5                   10                  15      


Ser Ser Ser Thr Ala Tyr Met Gln Leu Asn Ser Leu Thr Ser Glu Asp 
            20                  25                  30          


Ser Ala Val Tyr Phe Cys 
        35              


<210>  18
<211>  11
<212>  PRT
<213>  Mus musculus

<400>  18

Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser 
1               5                   10      


<210>  19
<211>  26
<212>  PRT
<213>  Mus musculus

<400>  19

Asp Ile Val Met Ser Gln Ser Pro Ser Ser Leu Gly Val Ser Val Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser 
            20                  25      


<210>  20
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  20

Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile 
1               5                   10                  15      


Tyr 
    


<210>  21
<211>  36
<212>  PRT
<213>  Mus musculus

<400>  21

Asn Arg Lys Ser Gly Val Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly 
1               5                   10                  15      


Thr Asp Phe Thr Leu Thr Ile Ser Ser Val Lys Ala Glu Asp Leu Ala 
            20                  25                  30          


Val Tyr Tyr Cys 
        35      


<210>  22
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  22

Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
1               5                   10  


<210>  23
<211>  5
<212>  PRT
<213>  Mus musculus

<400>  23

Asp His Ala Ile His 
1               5   


<210>  24
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  24

Tyr Phe Ser Pro Gly Asn Asp Asp Ile His Tyr Asn Glu Lys Phe Glu 
1               5                   10                  15      


Gly 
    


<210>  25
<211>  8
<212>  PRT
<213>  Mus musculus

<400>  25

Ser Tyr Asp Lys Asp Phe Asp Cys 
1               5               


<210>  26
<211>  17
<212>  PRT
<213>  Mus musculus

<400>  26

Lys Ser Ser Gln Ser Leu Leu Tyr Ser Thr Asn Gln Lys Asn Tyr Leu 
1               5                   10                  15      


Ala 
    


<210>  27
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  27

Trp Val Ser Asn Arg Lys Ser 
1               5           


<210>  28
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  28

Gly Tyr Thr Phe Thr Asp His 
1               5           


<210>  29
<211>  6
<212>  PRT
<213>  Mus musculus

<400>  29

Ser Pro Gly Asn Asp Asp 
1               5       


<210>  30
<211>  13
<212>  PRT
<213>  Mus musculus

<400>  30

Ser Gln Ser Leu Leu Tyr Ser Thr Asn Gln Lys Asn Tyr 
1               5                   10              


<210>  31
<211>  7
<212>  PRT
<213>  Mus musculus

<400>  31

Tyr Tyr Arg Tyr Pro Leu Thr 
1               5           


<210>  32
<211>  10
<212>  PRT
<213>  Mus musculus

<400>  32

Gly Tyr Thr Phe Thr Asp His Ala Ile His 
1               5                   10  


<210>  33
<211>  2
<212>  PRT
<213>  Mus musculus

<400>  33

Asp His 
1       


<210>  34
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CD3 CDR-H1 peptide

<400>  34

Thr Tyr Ala Met Asn 
1               5   


<210>  35
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CD3 CDR-H2 peptide

<400>  35

Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp Ser 
1               5                   10                  15      


Val Lys Gly 
            


<210>  36
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CD3 CDR-H3 peptide

<400>  36

His Gly Asn Phe Gly Asn Ser Tyr Val Ser Trp Phe Ala Tyr 
1               5                   10                  


<210>  37
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CD3 CDR-L1 peptide

<400>  37

Gly Ser Ser Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn 
1               5                   10                  


<210>  38
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CD3 CDR-L2  peptide

<400>  38

Gly Thr Asn Lys Arg Ala Pro 
1               5           


<210>  39
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CD3 CDR-L2 peptide

<400>  39

Ala Leu Trp Tyr Ser Asn Leu Trp Val 
1               5                   


<210>  40
<211>  125
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CD3 VH

<400>  40

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser Trp Phe 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 125 


<210>  41
<211>  109
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CD3 VL

<400>  41

Gln Ala Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Gly Ser Ser Thr Gly Ala Val Thr Thr Ser 
            20                  25                  30          


Asn Tyr Ala Asn Trp Val Gln Glu Lys Pro Gly Gln Ala Phe Arg Gly 
        35                  40                  45              


Leu Ile Gly Gly Thr Asn Lys Arg Ala Pro Gly Thr Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Ala 
65                  70                  75                  80  


Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn 
                85                  90                  95      


Leu Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu 
            100                 105                 


<210>  42
<211>  225
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic hIgG1 Fc region

<400>  42

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro 
225 


<210>  43
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic MUC1 VL-CL(RK)

<400>  43

Asp Ile Val Met Ser Gln Ser Pro Ser Ser Leu Gly Val Ser Val Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Tyr Ser 
            20                  25                  30          


Thr Asn Gln Lys Asn Tyr Gln Ser Leu Leu Tyr Ser Thr Asn Gln Lys 
        35                  40                  45              


Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser Pro Lys Leu 
    50                  55                  60                  


Leu Ile Tyr Trp Val Ser Asn Arg Lys Ser Gly Val Pro Asp Arg Phe 
65                  70                  75                  80  


Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Val 
                85                  90                  95      


Lys Ala Glu Asp Leu Ala Val Tyr Tyr Cys Gln Gln Tyr Tyr Arg Tyr 
            100                 105                 110         


Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys Arg Thr Val 
        115                 120                 125             


Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Arg Lys Leu Lys 
    130                 135                 140                 


Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
145                 150                 155                 160 


Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
                165                 170                 175     


Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
            180                 185                 190         


Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
        195                 200                 205             


Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
    210                 215                 220                 


Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230         


<210>  44
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic CD3 VH-CL

<400>  44

Glu Val Gln Leu Leu Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Thr Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Thr 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser Trp Phe 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser Ala Ser Val 
        115                 120                 125             


Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys 
    130                 135                 140                 


Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg 
145                 150                 155                 160 


Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn 
                165                 170                 175     


Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser 
            180                 185                 190         


Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys 
        195                 200                 205             


Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr 
    210                 215                 220                 


Lys Ser Phe Asn Arg Gly Glu Cys 
225                 230         


<210>  45
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  MUC1 VH-CH1(EE)-Fc (hole, P329G LALA)

<400>  45

Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Ala Ile His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Phe Ser Pro Gly Asn Asp Asp Ile His Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Glu Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Lys Arg Ser Tyr Asp Lys Asp Phe Asp Cys Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Leu Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Glu Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Glu Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220                 


Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser Val 
225                 230                 235                 240 


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
                245                 250                 255     


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
            260                 265                 270         


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
        275                 280                 285             


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
    290                 295                 300                 


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
305                 310                 315                 320 


Cys Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr Ile 
                325                 330                 335     


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Cys Thr Leu Pro 
            340                 345                 350         


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Ser Cys Ala 
        355                 360                 365             


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
    370                 375                 380                 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
385                 390                 395                 400 


Asp Gly Ser Phe Phe Leu Val Ser Lys Leu Thr Val Asp Lys Ser Arg 
                405                 410                 415     


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
            420                 425                 430         


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
        435                 440                 445         


<210>  46
<211>  672
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  synthetic MUC1 VH-CH1(EE)-CD3 VL-CH1-Fc (knob, P329G LALA)

<400>  46

Gln Val Gln Leu Gln Gln Ser Asp Ala Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Asp His 
            20                  25                  30          


Ala Ile His Trp Val Lys Gln Arg Pro Glu Gln Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Tyr Phe Ser Pro Gly Asn Asp Asp Ile His Tyr Asn Glu Lys Phe 
    50                  55                  60                  


Glu Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Asn Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys 
                85                  90                  95      


Lys Arg Ser Tyr Asp Lys Asp Phe Asp Cys Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Leu Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu 
        115                 120                 125             


Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys 
    130                 135                 140                 


Leu Val Glu Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser 
145                 150                 155                 160 


Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser 
                165                 170                 175     


Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser 
            180                 185                 190         


Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn 
        195                 200                 205             


Thr Lys Val Asp Glu Lys Val Glu Pro Lys Ser Cys Asp Gly Gly Gly 
    210                 215                 220                 


Gly Ser Gly Gly Gly Gly Ser Gln Ala Val Val Thr Gln Glu Pro Ser 
225                 230                 235                 240 


Leu Thr Val Ser Pro Gly Gly Thr Val Thr Leu Thr Cys Gly Ser Ser 
                245                 250                 255     


Thr Gly Ala Val Thr Thr Ser Asn Tyr Ala Asn Trp Val Gln Glu Lys 
            260                 265                 270         


Pro Gly Gln Ala Phe Arg Gly Leu Ile Gly Gly Thr Asn Lys Arg Ala 
        275                 280                 285             


Pro Gly Thr Pro Ala Arg Phe Ser Gly Ser Leu Leu Gly Gly Lys Ala 
    290                 295                 300                 


Ala Leu Thr Leu Ser Gly Ala Gln Pro Glu Asp Glu Ala Glu Tyr Tyr 
305                 310                 315                 320 


Cys Ala Leu Trp Tyr Ser Asn Leu Trp Val Phe Gly Gly Gly Thr Lys 
                325                 330                 335     


Leu Thr Val Leu Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro 
            340                 345                 350         


Leu Ala Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly 
        355                 360                 365             


Cys Leu Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn 
    370                 375                 380                 


Ser Gly Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln 
385                 390                 395                 400 


Ser Ser Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser 
                405                 410                 415     


Ser Leu Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser 
            420                 425                 430         


Asn Thr Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr 
        435                 440                 445             


His Thr Cys Pro Pro Cys Pro Ala Pro Glu Ala Ala Gly Gly Pro Ser 
    450                 455                 460                 


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
465                 470                 475                 480 


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
                485                 490                 495     


Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
            500                 505                 510         


Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val 
        515                 520                 525             


Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
    530                 535                 540                 


Lys Cys Lys Val Ser Asn Lys Ala Leu Gly Ala Pro Ile Glu Lys Thr 
545                 550                 555                 560 


Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
                565                 570                 575     


Pro Pro Cys Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Trp Cys 
            580                 585                 590         


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
        595                 600                 605             


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp 
    610                 615                 620                 


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
625                 630                 635                 640 


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
                645                 650                 655     


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
            660                 665                 670         


<210>  47
<211>  60
<212>  PRT
<213>  Homo sapiens

<400>  47

Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro 
1               5                   10                  15      


Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly 
            20                  25                  30          


Ser Thr Ala Pro Pro Ala His Gly Val Thr Ser Ala Pro Asp Thr Arg 
        35                  40                  45              


Pro Ala Pro Gly Ser Thr Ala Pro Pro Ala His Gly 
    50                  55                  60  


<210>  48
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  48

Pro Asp Thr Ser Ala Ala Pro Gly Ser Thr Ala Pro Pro Ala His Val 
1               5                   10                  15      


Val Thr Ser Ala Pro 
            20      


<210>  49
<211>  21
<212>  PRT
<213>  Macaca fascicularis

<400>  49

Pro Asp Thr Ser Ala Ala Pro Gly Ser Thr Gly Pro Pro Ala His Val 
1               5                   10                  15      


Val Thr Ser Ala Pro 
            20      


<210>  50
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  50

Val Thr Ser Ala Pro Asp Thr Arg Pro Ala Pro Gly Ser Thr Ala Pro 
1               5                   10                  15      


Pro Ala His Gly 
            20  


<210>  51
<211>  20
<212>  PRT
<213>  Homo sapiens

<400>  51

Pro Thr Thr Thr Pro Ile Ser Thr Thr Thr Met Val Thr Pro Thr Pro 
1               5                   10                  15      


Thr Pro Thr Cys 
            20  


<210>  52
<211>  21
<212>  PRT
<213>  Homo sapiens

<400>  52

Cys Pro Leu Pro Val Thr Asp Thr Ser Ser Ala Ser Thr Gly His Ala 
1               5                   10                  15      


Thr Pro Leu Pro Val 
            20      


