                         SEQUENCE LISTING

<110>  National University of Singapore
 
<120>  Ligation and/or assembly of nucleic acid molecules

<130>  SP100162WO

<150>  SG10201708764U
<151>  2017-10-25

<160>  470   

<170>  PatentIn version 3.5

<210>  1
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  G-gRNA-nupG F


<220>
<221>  t
<222>  (2)..(2)
<223>  phosphothioate-modified T

<400>  1
gtacgagtta atcaatatca cagttttaga gctagaaata g                           41


<210>  2
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  gRNA-T R


<220>
<221>  t
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  2
atctagagaa ttcaaaaaaa g                                                 21


<210>  3
<211>  41
<212>  DNA
<213>  Artificial

<220>
<223>  G-gRNA-aslA F


<220>
<221>  t
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  3
gtgcagaact tgagaaaaaa acgttttaga gctagaaata g                           41


<210>  4
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  G-gRNA-melB F


<220>
<221>  t
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  4
gtctaccatt tgttaattat gtgttttaga gctagaaata g                           41


<210>  5
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  G-gRNA-rcsB F


<220>
<221>  n
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  5
gtaatcactt gagcaaattg aggttttaga gctagaaata g                           41


<210>  6
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  G-gRNA-tyrR F


<220>
<221>  t
<222>  (2)..(2)
<223>  phosphothioate-modified T

<400>  6
gtttaatacc gagcgttcaa aagttttaga gctagaaata g                           41


<210>  7
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  G-gRNA-pheA F


<220>
<221>  t
<222>  (2)..(2)
<223>  phosphothioate-modified T

<400>  7
gttttgagca attcattgaa aggttttaga gctagaaata g                           41


<210>  8
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  G-gRNA-ptsI F


<220>
<221>  t
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  8
gtgaagttga tttctttagt atgttttaga gctagaaata g                           41


<210>  9
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-nupG-HF0.5 F


<220>
<221>  t
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  9
gttgatcctg ccagcaata                                                    19


<210>  10
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  nupG-HF0.5-T R


<220>
<221>  c
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  10
acatcgtgat gcggatgag                                                    19


<210>  11
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-nupG-HF1.0 F


<220>
<221>  n
<222>  (2)..(2)
<223>  phosphothioate-modified a

<220>
<221>  a
<222>  (2)..(2)
<223>  phosphothioate-modified a

<400>  11
gaccatcgcc gggacagaac c                                                 21


<210>  12
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-nupG-HF1.5 F


<220>
<221>  n
<222>  (2)..(2)
<223>  phosphothioate-modified T

<220>
<221>  t
<222>  (2)..(2)
<223>  phosphothioate-modified T

<400>  12
gtgcaacgtg aagcagaagg t                                                 21


<210>  13
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-aslA-HF0.5 F


<220>
<221>  c
<222>  (2)..(2)
<223>  phosphothioate-modified C

<400>  13
gcaccgtaaa cggctctgc                                                    19


<210>  14
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  aslA-HF0.5-T R


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  14
agtttcatgt catcaaaatg                                                   20


<210>  15
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-aslA-HF1.0 F


<220>
<221>  c
<222>  (2)..(2)
<223>  phosphothioate-modified C

<400>  15
gccagtacga cgatcgcct                                                    19


<210>  16
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-melB-HF1.0 F


<220>
<221>  c
<222>  (2)..(2)
<223>  phosphothioate-modified C

<400>  16
gcccaatggc gatgaatacc t                                                 21


<210>  17
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  melB-HF1.0-T R


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphothioate-modified G

<400>  17
agctgttacc aacgcccgcc t                                                 21


<210>  18
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-rcsB-HF1.0 F


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphothioate-modified G

<400>  18
ggttagcgaa catgcttgcg g                                                 21


<210>  19
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  rcsB-HF1.0-T R


<220>
<221>  t
<222>  (2)..(2)
<223>  phosphothioate-modified T

<400>  19
attgctacag caagctcttg a                                                 21


<210>  20
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-tyrR-HF0.5 F


<220>
<221>  c
<222>  (2)..(2)
<223>  phosphothioate-modified C

<400>  20
gcagcccgct ggcgttggt                                                    19


<210>  21
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  Antcagcacccgatattgcat


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphothioate-modified G

<400>  21
agtcagcacc cgatattgca t                                                 21


<210>  22
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  G-pheA-HF0.5 F


<220>
<221>  c
<222>  (2)..(2)
<223>  phosphothioate-modified C

<400>  22
gcatgtcgca gaccgtctcg                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  pheA-HF0.5-T R


<220>
<221>  c
<222>  (2)..(2)
<223>  phosphothioate-modified C

<400>  23
acgaaacgcc tcccattcag                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  G-ptsI-HF0.7 F


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphothioate-modified G

<400>  24
ggcccgcata aaattcaggg                                                   20


<210>  25
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  ptsI-HF0.7-T R


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphothioate-modified g

<400>  25
aggaactaaa gtctagcctg g                                                 21


<210>  26
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-nupG-HT0.5 F


<220>
<221>  t
<222>  (2)..(2)
<223>  phosphothioate-modified T

<400>  26
gttacgcaaa gaaaaacgg                                                    19


<210>  27
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  nupG-HT0.5-T R


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphothioate-modified G

<400>  27
agccgctggt tgaggtgtt                                                    19


<210>  28
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  nupG-HT1.0-T R


<220>
<221>  a
<222>  (2)..(2)
<223>  phosphothioate-modified  A

<400>  28
aacgccttta tgctccatgc t                                                 21


<210>  29
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  nupG-HT1.5-T R


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphothioate-modified G

<400>  29
agcgacgccg gtctatctgg a                                                 21


<210>  30
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-aslA-HT0.5 F


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphothioate-modified G

<400>  30
ggccggcgct atcgctgag                                                    19


<210>  31
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  aslA-HT0.5-T R


<220>
<221>  c
<222>  (2)..(2)
<223>  phosphothioate-modified C

<400>  31
acactatgtt tatccgcaa                                                    19


<210>  32
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  aslA-HT1.0-T R


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphothioate-modified G

<400>  32
agcccgcctg agatccaca                                                    19


<210>  33
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-melB-HT1.0 F


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphothiooate-modifed G

<400>  33
ggtgcagtga gtgatgtgaa a                                                 21


<210>  34
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  melB-HT1.0-T R


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphothioate-modified G

<400>  34
agggtatgga agctatctgg a                                                 21


<210>  35
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-rcsB-HT1.0 F


<220>
<221>  t
<222>  (2)..(2)
<223>  phosphothioate-modified T

<400>  35
gtcacctgta ggccagataa g                                                 21


<210>  36
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  rcsB-HT1.0-T R


<220>
<221>  a
<222>  (2)..(2)
<223>  phosphothioate-modified A

<400>  36
aattcagaac cgggaatggg c                                                 21


<210>  37
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-tyrR-HT0.5 F


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphotrohioate-modified G

<400>  37
ggcgcgaata tgcctgatg                                                    19


<210>  38
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  tyrR-HT0.5-T R


<220>
<221>  c
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  38
acatcccgca ggcgggtag                                                    19


<210>  39
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-pheA-HT0.5 F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  39
gttactggcg attgtcattc g                                                 21


<210>  40
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  pheA-HT0.5-T R


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphorothioate-modified A

<400>  40
aaaatgggcc attacaggcc                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  G-ptsI-HT0.7 F


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphorothioate-modified A

<400>  41
gagcgcatca cttccagtac                                                   20


<210>  42
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  ptsI-HT0.7-T R


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  42
ataacgataa gagtagggca c                                                 21


<210>  43
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  G-spectR F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  43
gtcgacctgc agaagctt                                                     18


<210>  44
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  spectR-T R


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  44
acgttaaggg attttggt                                                     18


<210>  45
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  G-ampR F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  45
gtttctacaa actctttt                                                     18


<210>  46
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-repA/p5 F


<220>
<221>  c
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  46
gccgttttca tctgtgcata t                                                 21


<210>  47
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  repA/p5-T R


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  47
atccttttgt aatactgcgg a                                                 21


<210>  48
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-p15A F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  48
gttgttcagc tactgacgg                                                    19


<210>  49
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  p15A-T R


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  49
agacatcacc gatgggga                                                     18


<210>  50
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  G-pMB1 F


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphorothioate-modified A

<400>  50
gagttttcgt tccactga                                                     18


<210>  51
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  pMB1-T R


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  51
aggatccagc atatgcgg                                                     18


<210>  52
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-pUC F


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  52
ggctcactca aaggcggta                                                    19


<210>  53
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  pUC-T R


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphorothioate-modified A

<400>  53
aattaccgcc tttgagtga                                                    19


<210>  54
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  G-pLac F


<220>
<221>  c
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  54
gcaacgcaat taatgtgagt                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  pLac-T R


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  55
attgttatcc gctcacaatt                                                   20


<210>  56
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-lacIT7 F


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  56
ggaaactacc cataatacaa g                                                 21


<210>  57
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  LacIT7-T R


<220>
<221>  g
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  57
agaggggaat tgttatccgc tcacaattcc cctatagtga                             40


<210>  58
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  G-t7t F


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  58
gggctgctaa caaagccc                                                     18


<210>  59
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  t7t-T R


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  59
aggcaccgtc accctggat                                                    19


<210>  60
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  lacI-T R


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  60
atcccggaca ccatcgaat                                                    19


<210>  61
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-dxs F


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphorothioate-modified A

<400>  61
gagttttgat attgccaaat a                                                 21


<210>  62
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  dxs-T R


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  62
atgccagcca ggccttg                                                      17


<210>  63
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-idi F


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  63
gcaaacggaa cacgtcattt t                                                 21


<210>  64
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  idi-T R


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  64
atttaagctg ggtaaatgca g                                                 21


<210>  65
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  G-ispA F


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphrothioate-modified G

<400>  65
ggactttccg cagcaact                                                     18


<210>  66
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  ispA-T R


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  66
atttattacg ctggatgatg t                                                 21


<210>  67
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  G-ValC F


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  67
ggccgagatg ttcaacgg                                                     18


<210>  68
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  ValC-T R


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  68
aggggatgat gggctcg                                                      17


<210>  69
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-tyrR mutant F


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  69
ggttgctgaa ttgaccgcat t                                                 21


<210>  70
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  tyrR mutant-T R


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  70
actggcgatt gtcattcgc                                                    19


<210>  71
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-aroG mutant


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphorothioate-modifeid A

<400>  71
gaattatcag aacgacgatt t                                                 21


<210>  72
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  aroG mutant-T R


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  72
acccgcgacg cgctttta                                                     18


<210>  73
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  RBS1

<400>  73
tagaaataat tttgtttaac tttaagaagg agatatacat atg                         43


<210>  74
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  RBS2stop

<400>  74
taaccgttca tttatcacaa aaggattgtt cgatg                                  35


<210>  75
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  RBS3stop

<400>  75
tgattcacac aggaaacagc tatg                                              24


<210>  76
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  RBS4stop

<400>  76
taaattaatt gttctttttt caggtgaagg ttcccatg                               38


<210>  77
<211>  34
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119

<400>  77
ttgacagcta gctcagtcct aggtataata ctag                                   34


<210>  78
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  N21

<400>  78
ttccctcgac tcacacttgg g                                                 21


<210>  79
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  N22

<400>  79
ttcacacaac atagccacgg g                                                 21


<210>  80
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  N23

<400>  80
ttcaaacaga aaggccatgg g                                                 21


<210>  81
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  N24

<400>  81
ttcctcatgg attctacggg g                                                 21


<210>  82
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  N31

<400>  82
tgatgggctg aagggtttaa g                                                 21


<210>  83
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  N32

<400>  83
tgtcccatca cagcttacaa g                                                 21


<210>  84
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  LK3

<400>  84
tcaggctcgt cttcttcagg g                                                 21


<210>  85
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  T-N21'_pT2 F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  85
ttccctcgac tcacactttc gagttcatgt gcagctcc                               38


<210>  86
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  pT2_N21''-G R


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  86
cccaagtgtg agtcgaggtc agctcactca aaggcggt                               38


<210>  87
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  T-N22'_pT2 F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  87
ttcacacaac atagccactc gagttcatgt gcagctcc                               38


<210>  88
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  pT2_N22''-G R


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphothioate-modified C

<400>  88
cccgtggcta tgttgtgttc agctcactca aaggcggt                               38


<210>  89
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  T-N23'_pT2 F


<220>
<221>  T
<222>  (2)..(2)
<223>  phsophorothioate-modified T

<400>  89
ttcaaacaga aaggccattc gagttcatgt gcagctcc                               38


<210>  90
<211>  39
<212>  DNA
<213>  artificial

<220>
<223>  pT2_N23''-G R


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  90
cccatggcct ttctgtttgt cagctcactc aaaggcggt                              39


<210>  91
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  T-N24'_pT2 F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  91
ttcctcatgg attctacgtc gagttcatgt gcagctcc                               38


<210>  92
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  pT2_N24''-G R


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  92
ccccgtagaa tccatgagtc agctcactca aaggcggt                               38


<210>  93
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  T-pJ23119'_pT2 F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  93
ttgacagcta gctcagtcct aggtcgagtt catgtgcagc tcc                         43


<210>  94
<211>  46
<212>  DNA
<213>  artificial

<220>
<223>  pT2_pJ23119''-G R


<220>
<221>  T
<222>  (2)..(2)
<223>  phoshorothioate-modified T

<400>  94
ctagtattat acctaggact gagctatcag ctcactcaaa ggcggt                      46


<210>  95
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N21s-Bff

<400>  95
cctcgactca cacttgg                                                      17


<210>  96
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  N21s-Bfr


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  96
caagtgtgag tcgagg                                                       16


<210>  97
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N22s-Bff

<400>  97
acacaacata gccacgg                                                      17


<210>  98
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  N22s-Bfr


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  98
cgtggctatg ttgtgt                                                       16


<210>  99
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N23s-Bff

<400>  99
caaacagaaa ggccatgg                                                     18


<210>  100
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N23s-Bfr


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  100
catggccttt ctgtttg                                                      17


<210>  101
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N24s-Bff

<400>  101
ctcatggatt ctacggg                                                      17


<210>  102
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  N24s-Bfr


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  102
ccgtagaatc catgag                                                       16


<210>  103
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119s-Bff

<400>  103
tagctcagtc ctaggtataa tactag                                            26


<210>  104
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119s-Bfr


<220>
<221>  T
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  104
tagtattata cctaggactg agcta                                             25


<210>  105
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  N21s-Brf


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  105
ccctcgactc acactt                                                       16


<210>  106
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  N21s-Brr

<400>  106
agtgtgagtc gaggga                                                       16


<210>  107
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  N22s-Brf


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  107
cacacaacat agccac                                                       16


<210>  108
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N22s-Brr

<400>  108
gtggctatgt tgtgtga                                                      17


<210>  109
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  N23s-Brf


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  109
caaacagaaa ggccat                                                       16


<210>  110
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N23s-Brr

<400>  110
atggcctttc tgtttga                                                      17


<210>  111
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  N24s-Brf


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  111
cctcatggat tctacg                                                       16


<210>  112
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N24s-Brr

<400>  112
cgtagaatcc atgagga                                                      17


<210>  113
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119s-Brf


<220>
<221>  T
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  113
tgacagctag ctcagtccta gg                                                22


<210>  114
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119s-Brr

<400>  114
cctaggactg agctagctgt caa                                               23


<210>  115
<211>  69
<212>  DNA
<213>  artificial

<220>
<223>  RBS1-Bf-SL

<400>  115
atatgtatat ctccttctta aagttaaaca atatgttttg tttaacttta agaaggagat       60

atacatatg                                                               69


<210>  116
<211>  65
<212>  DNA
<213>  artificial

<220>
<223>  RBS1-Br-SL

<400>  116
agaaataatt ttgtttaact ttaagaaggt ctactccttc ttaaagttaa acaaaattat       60

ttcta                                                                   65


<210>  117
<211>  63
<212>  DNA
<213>  artificial

<220>
<223>  RBS2stop-Bf-SL

<400>  117
atcgaacaat ccttttgtga taaatgaatc ggttttcatt tatcacaaaa ggattgttcg       60

atg                                                                     63


<210>  118
<211>  55
<212>  DNA
<213>  artificial

<220>
<223>  RBS2stop-Br-SL

<400>  118
aaccgttcat ttatcacaaa aggatatcac tccttttgtg ataaatgaac ggtta            55


<210>  119
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  RBS3stop-Bf-SL

<400>  119
atagctgttt cctgtgtgcc tggacacagg aaacagctat g                           41


<210>  120
<211>  47
<212>  DNA
<213>  artificial

<220>
<223>  RBS3stop-Br-SL

<400>  120
gattcacaca ggaaacagct tcttcgagct gtttcctgtg tgaatca                     47


<210>  121
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  RBS4stop-Bf-SL

<400>  121
atgggaacct tcacctgaag ttacaggtga aggttcccat g                           41


<210>  122
<211>  75
<212>  DNA
<213>  artificial

<220>
<223>  RBS4stop-Br-SL

<400>  122
aaattaattg ttcttttttc aggtgaaggt tccctcacat gggaaccttc acctgaaaaa       60

agaacaatta attta                                                        75


<210>  123
<211>  57
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119-Bf-SL

<400>  123
tagtattata cctaggactg agctaagagg gtagctcagt cctaggtata atactag          57


<210>  124
<211>  50
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119-Br-SL

<400>  124
gacagctagc tcagtcctag ggcacagcct aggactgagc tagctgtcaa                  50


<210>  125
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N21-Bf-SL

<400>  125
ccaagtgtga gtcgaggaag ggccctcgac tcacacttgg g                           41


<210>  126
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N21-Br-SL

<400>  126
tccctcgact cacacttgcg agaaagtgtg agtcgaggga a                           41


<210>  127
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N22-Bf-SL

<400>  127
ccgtggctat gttgtgtcgt attacacaac atagccacgg g                           41


<210>  128
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N22-Br-SL

<400>  128
tcacacaaca tagccacttg ctggtggcta tgttgtgtga a                           41


<210>  129
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  N23-Bf-SL

<400>  129
ccatggcctt tctgtttgac catacaaaca gaaaggccat ggg                         43


<210>  130
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N23-Br-SL

<400>  130
tcaaacagaa aggccatcat tcaatggcct ttctgtttga a                           41


<210>  131
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N24-Bf-SL

<400>  131
cccgtagaat ccatgagaac ccgctcatgg attctacggg g                           41


<210>  132
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N24-Br-SL

<400>  132
tcctcatgga ttctacgact atgcgtagaa tccatgagga a                           41


<210>  133
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N31-Bf-SL

<400>  133
ttaaaccctt cagcccacac acatgggctg aagggtttaa g                           41


<210>  134
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N31-Br-SL

<400>  134
gatgggctga agggtttcac acaaaaccct tcagcccatc a                           41


<210>  135
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N32-Bf-SL

<400>  135
ttgtaagctg tgatggggcc tggcccatca cagcttacaa g                           41


<210>  136
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N32-Br-SL

<400>  136
gtcccatcac agcttacgct tcggtaagct gtgatgggac a                           41


<210>  137
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  LK3-Bf-SL

<400>  137
cctgaagaag acgagcccac acaggctcgt cttcttcagg g                           41


<210>  138
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  LK3-Br-SL

<400>  138
caggctcgtc ttcttcacac acatgaagaa gacgagcctg a                           41


<210>  139
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  RBS1-Bff


<220>
<221>  T
<222>  (10)..(10)
<223>  phosphorothioate-modified T

<220>
<221>  A
<222>  (22)..(22)
<223>  phosphorothioate-modified A

<400>  139
ttgtttaact ttaagaagga gatatacata tg                                     32


<210>  140
<211>  29
<212>  DNA
<213>  artificial

<220>
<223>  RBS2stop-Bff


<220>
<221>  C
<222>  (10)..(10)
<223>  phosphothioate-modified C

<220>
<221>  T
<222>  (20)..(20)
<223>  phosphothioate-modified T

<400>  140
ttcatttatc acaaaaggat tgttcgatg                                         29


<210>  141
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  RBS3-Bff


<220>
<221>  A
<222>  (8)..(8)
<223>  phosphothoate-modified A

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphothoate-modified A

<400>  141
acacaggaaa cagctatg                                                     18


<210>  142
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  RBS4-Bff


<220>
<221>  G
<222>  (9)..(9)
<223>  phosphothioate-modified G

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphothioate-modified A

<400>  142
caggtgaagg ttcccatg                                                     18


<210>  143
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119-Bff


<220>
<221>  G
<222>  (8)..(8)
<223>  phosphothioate-modified G

<220>
<221>  T
<222>  (16)..(16)
<223>  phosphothioate-modified T

<400>  143
tagctcagtc ctaggtataa tactag                                            26


<210>  144
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N21-Bff


<220>
<221>  T
<222>  (8)..(8)
<223>  phosphothioate-modified T

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphothioate-modified G

<400>  144
cctcgactca cacttggg                                                     18


<210>  145
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N22-Bff


<220>
<221>  A
<222>  (8)..(8)
<223>  phosphothioate-modified A

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphothioate-modified G

<400>  145
acacaacata gccacggg                                                     18


<210>  146
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N23-Bff


<220>
<221>  A
<222>  (9)..(9)
<223>  phosphothioate-modified A

<220>
<221>  G
<222>  (17)..(17)
<223>  phosphothioate-modified G

<400>  146
caaacagaaa ggccatggg                                                    19


<210>  147
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N24-Bff


<220>
<221>  A
<222>  (8)..(8)
<223>  phosphothioate-modified A

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphothioate-modified G

<400>  147
ctcatggatt ctacgggg                                                     18


<210>  148
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N31-Bff


<220>
<221>  A
<222>  (8)..(8)
<223>  phosphothioate-modified A

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphothioate-modified A

<400>  148
tgggctgaag ggtttaag                                                     18


<210>  149
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N32-Bff


<220>
<221>  A
<222>  (9)..(9)
<223>  phosphothioate-modified A

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphothioate-modified A

<400>  149
cccatcacag cttacaag                                                     18


<210>  150
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  LK3-Bff


<220>
<221>  C
<222>  (8)..(8)
<223>  phosphothioate-modified C

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphothioate-modified G

<400>  150
ggctcgtctt cttcaggg                                                     18


<210>  151
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  RBS1-Brr


<220>
<221>  a
<222>  (10)..(10)
<223>  phosphorothioate-modified A

<220>
<221>  a
<222>  (20)..(20)
<223>  phosphorothioate-modified A

<400>  151
ccttcttaaa gttaaacaaa attatttcta                                        30


<210>  152
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  RBS2stop-Brr


<220>
<221>  G
<222>  (10)..(10)
<223>  phosphorothioate-modified G

<220>
<221>  C
<222>  (20)..(20)
<223>  phosphorothioate-modified C

<400>  152
tccttttgtg ataaatgaac ggtta                                             25


<210>  153
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  RBS3stop-Brr


<220>
<221>  C
<222>  (9)..(9)
<223>  phosphorotioage-modified C

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorotioage-modified G

<400>  153
agctgtttcc tgtgtgaatc a                                                 21


<210>  154
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  RBS4stop-Brr


<220>
<221>  T
<222>  (9)..(9)
<223>  phosphorothioage-modified T

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphorothioage-modified A

<400>  154
gggaaccttc acctgaaaaa agaacaatta attta                                  35


<210>  155
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119-Brr


<220>
<221>  C
<222>  (8)..(8)
<223>  phosphorothioate-modified C

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  155
cctaggactg agctagctgt caa                                               23


<210>  156
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N21-Brr


<220>
<221>  A
<222>  (8)..(8)
<223>  phosphorothioate-modified A

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  156
aagtgtgagt cgagggaa                                                     18


<210>  157
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N22-Brr


<220>
<221>  T
<222>  (8)..(8)
<223>  phosphorothioate-modified T

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  157
gtggctatgt tgtgtgaa                                                     18


<210>  158
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N23-Brr


<220>
<221>  T
<222>  (9)..(9)
<223>  phosphorothioate-modified T

<220>
<221>  A
<222>  (17)..(17)
<223>  phosphorothioate-modified A

<400>  158
atggcctttc tgtttgaa                                                     18


<210>  159
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N24-Brr


<220>
<221>  T
<222>  (8)..(8)
<223>  phosphorothioate-modified T

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified T

<400>  159
cgtagaatcc atgaggaa                                                     18


<210>  160
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N31-Brr


<220>
<221>  T
<222>  (8)..(8)
<223>  phosphorothioate-modified T

<220>
<221>  T
<222>  (16)..(16)
<223>  phosphorothioate-modified T

<400>  160
aaacccttca gcccatca                                                     18


<210>  161
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N32-Brr


<220>
<221>  T
<222>  (9)..(9)
<223>  phosphorothioate-modified T

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphorothioate-modified A

<400>  161
gtaagctgtg atgggaaca                                                    19


<210>  162
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  LK3-Brr


<220>
<221>  G
<222>  (8)..(8)
<223>  phosphorothioate-modified G

<220>
<221>  T
<222>  (16)..(16)
<223>  phosphorothioate-modified T

<400>  162
tgaagaagac gagcctga                                                     18


<210>  163
<211>  171
<212>  DNA
<213>  artificial

<220>
<223>  (pJ23119)gRNA-nupG(N23)

<400>  163
ttgacagcta gctcagtcct aggtataata ctagtacgag ttaatcaata tcacagtttt       60

agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac      120

cgagtcggtg ctttttttga attctctaga ttcaaacaga aaggccatgg g               171


<210>  164
<211>  171
<212>  DNA
<213>  artificial

<220>
<223>  (pJ23119)gRNA-aslA(N23)

<400>  164
ttgacagcta gctcagtcct aggtataata ctagttccag atcttccata tatttgtttt       60

agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac      120

cgagtcggtg ctttttttga attctctaga ttcaaacaga aaggccatgg g               171


<210>  165
<211>  171
<212>  DNA
<213>  artificial

<220>
<223>  (pJ23119)gRNA-melB(N23)

<400>  165
ttgacagcta gctcagtcct aggtataata ctagtctacc atttgttaat tatgtgtttt       60

agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac      120

cgagtcggtg ctttttttga attctctaga ttcaaacaga aaggccatgg g               171


<210>  166
<211>  171
<212>  DNA
<213>  artificial

<220>
<223>  (pJ23119)gRNA-rcsB(N23)

<400>  166
ttgacagcta gctcagtcct aggtataata ctagtaatca cttgagcaaa ttgaggtttt       60

agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac      120

cgagtcggtg ctttttttga attctctaga ttcaaacaga aaggccatgg g               171


<210>  167
<211>  171
<212>  DNA
<213>  artificial

<220>
<223>  (pJ23119)gRNA-tyrR(N23)

<400>  167
ttgacagcta gctcagtcct aggtataata ctagtttaat accgagcgtt caaaagtttt       60

agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac      120

cgagtcggtg ctttttttga attctctaga ttcaaacaga aaggccatgg g               171


<210>  168
<211>  171
<212>  DNA
<213>  artificial

<220>
<223>  (pJ23119)gRNA-pheA(N23)

<400>  168
ttgacagcta gctcagtcct aggtataata ctagttttga gcaattcatt gaaaggtttt       60

agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac      120

cgagtcggtg ctttttttga attctctaga ttcaaacaga aaggccatgg g               171


<210>  169
<211>  171
<212>  DNA
<213>  artificial

<220>
<223>  (pJ23119)gRNA-ptsI(N23)

<400>  169
ttgacagcta gctcagtcct aggtataata ctagtgaagt tgatttcttt agtatgtttt       60

agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac      120

cgagtcggtg ctttttttga attctctaga ttcaaacaga aaggccatgg g               171


<210>  170
<211>  529
<212>  DNA
<213>  artificial

<220>
<223>  (N23)nupG-HF0.5(N24)

<400>  170
ttcaaacaga aaggccatgg gttgatcctg ccagcaatat tgataccggc accgcgtatc       60

tggcgatgct gaacaatgtt tatctcggcg gaattgataa cccaacatcg cggcgttatg      120

ccgtcatcac cgcctataac ggcggcgcag gcagcgtgct gcgagtcttt tcgaatgata      180

agattcaggc tgccaatatt attaacacca tgacgccggg cgatgtttat cagacgctga      240

cgacccgcca tccctctgcg gaatctcgcc gttatcttta taaagtgaat accgcgcaaa      300

aatcctaccg ccgccgataa ttccattaac cgcccctgac gatgctcagg ggcaaaaatg      360

ttatccacat cacaatttcg ttttgcaaat tgggaatgtt tgcaattatt tgccacaggt      420

aacaaaaaac cagtccgcga agttgataga atcccatcat ctcgcacggt caaatgtgct      480

ttttcaaaca ctcatccgca tcacgatgtt cctcatggat tctacgggg                  529


<210>  171
<211>  1046
<212>  DNA
<213>  artificial

<220>
<223>  (N23)nupG-HF1.0(N24)

<400>  171
ttcaaacaga aaggccatgg gaccatcgcc gggacagaac ctgccgcgca tttgcgccgg       60

gcaattatca aaacgttatt gatgggtgac gatccgagtt cggtcgatct ctattccgac      120

gttgatgata ttacgatttc gaaagaacct ttcctttacg gtcaggtggt ggacaacacc      180

gggcagccga ttcgctggga aggtcgcgca agcaacttcg cggattatct gctgaaaaac      240

cgtctgaaga gccgcagcaa cgggctgcgt atcatctaca gcgtcaccat taacatggtg      300

ccgaaccacc ttgataaacg tgcgcacaaa tatctcggca tggtccgcca ggcgtcacgg      360

aaatatggcg ttgatgagtc gctgattctg gcaattatgc agaccgaatc ttcctttaac      420

ccgtatgcgg tcagccgttc cgatgcgctg ggattaatgc aggtggtaca acatactgcc      480

gggaaagatg tgttccgctc gcaggggaaa tccggcacgc cgagccgcag tttcttgttt      540

gatcctgcca gcaatattga taccggcacc gcgtatctgg cgatgctgaa caatgtttat      600

ctcggcggaa ttgataaccc aacatcgcgg cgttatgccg tcatcaccgc ctataacggc      660

ggcgcaggca gcgtgctgcg agtcttttcg aatgataaga ttcaggctgc caatattatt      720

aacaccatga cgccgggcga tgtttatcag acgctgacga cccgccatcc ctctgcggaa      780

tctcgccgtt atctttataa agtgaatacc gcgcaaaaat cctaccgccg ccgataattc      840

cattaaccgc ccctgacgat gctcaggggc aaaaatgtta tccacatcac aatttcgttt      900

tgcaaattgg gaatgtttgc aattatttgc cacaggtaac aaaaaaccag tccgcgaagt      960

tgatagaatc ccatcatctc gcacggtcaa atgtgctttt tcaaacactc atccgcatca     1020

cgatgttcct catggattct acgggg                                          1046


<210>  172
<211>  1622
<212>  DNA
<213>  artificial

<220>
<223>  (N23)nupG-HF1.5(N24)

<400>  172
ttcaaacaga aaggccatgg gtgcaacgtg aagcagaagg tcaggatttt cagctgtacc       60

ccggcgagct gggaaaacgc atctataacg agatctccaa agaagcctgg gcgcagtggc      120

agcacaagca aaccatgctg attaatgaaa agaaactcaa catgatgaat gccgagcacc      180

gcaagctgct tgagcaggag atggtcaact tcctgttcga gggtaaagag gtgcatatcg      240

agggctatac gccggaagat aaaaaataaa aacagtgccg gagcacgcct ccggcaactt      300

gcataaaaac aaacacaaca cgcacccgga atgatgaaaa aatatctcgc gctggctttg      360

attgcgccgt tgctcatctc ctgttcgacg accaaaaaag gcgataccta taacgaagcc      420

tgggtcaaag ataccaacgg ttttgatatt ctgatggggc aatttgccca caatattgag      480

aacatctggg gcttcaaaga ggtggtgatc gctggtccta aggactacgt gaaatacacc      540

gatcaatatc agacccgcag ccacatcaac ttcgatgacg gtacgattac tatcgaaacc      600

atcgccggga cagaacctgc cgcgcatttg cgccgggcaa ttatcaaaac gttattgatg      660

ggtgacgatc cgagttcggt cgatctctat tccgacgttg atgatattac gatttcgaaa      720

gaacctttcc tttacggtca ggtggtggac aacaccgggc agccgattcg ctgggaaggt      780

cgcgcaagca acttcgcgga ttatctgctg aaaaaccgtc tgaagagccg cagcaacggg      840

ctgcgtatca tctacagcgt caccattaac atggtgccga accaccttga taaacgtgcg      900

cacaaatatc tcggcatggt ccgccaggcg tcacggaaat atggcgttga tgagtcgctg      960

attctggcaa ttatgcagac cgaatcttcc tttaacccgt atgcggtcag ccgttccgat     1020

gcgctgggat taatgcaggt ggtacaacat actgccggga aagatgtgtt ccgctcgcag     1080

gggaaatccg gcacgccgag ccgcagtttc ttgtttgatc ctgccagcaa tattgatacc     1140

ggcaccgcgt atctggcgat gctgaacaat gtttatctcg gcggaattga taacccaaca     1200

tcgcggcgtt atgccgtcat caccgcctat aacggcggcg caggcagcgt gctgcgagtc     1260

ttttcgaatg ataagattca ggctgccaat attattaaca ccatgacgcc gggcgatgtt     1320

tatcagacgc tgacgacccg ccatccctct gcggaatctc gccgttatct ttataaagtg     1380

aataccgcgc aaaaatccta ccgccgccga taattccatt aaccgcccct gacgatgctc     1440

aggggcaaaa atgttatcca catcacaatt tcgttttgca aattgggaat gtttgcaatt     1500

atttgccaca ggtaacaaaa aaccagtccg cgaagttgat agaatcccat catctcgcac     1560

ggtcaaatgt gctttttcaa acactcatcc gcatcacgat gttcctcatg gattctacgg     1620

gg                                                                    1622


<210>  173
<211>  529
<212>  DNA
<213>  artificial

<220>
<223>  (N23)aslA-HF0.5(N24)

<400>  173
ttcaaacaga aaggccatgg gcaccgtaaa cggctctgcg tcattccgga gtttatgagg       60

cactaaggcg aacataagag atggaatgag catctactcg tttattatgc cacagagaat      120

cgggaaataa catcccttaa cacttgttat gagataattc tgtaatcctc tttgcttcct      180

gagtaataac ttcctgagtg aatatttaac ctgagcttga tcctacacat acttattatg      240

aatgataaaa ttcattcaat taataacaca tatattaatt gccgttaaaa ctaaaaacag      300

catcaataat caacgcgata taataaacct gccttacata tcaactgcgc cagaggtagg      360

attgaaaacg ctctcctgat tttccaattc attttctgga taaataaata atttattttt      420

gtcactatta tttatgtaat catcctgtca gggagaggga tctcaattat caatgcttaa      480

ttacgtcatc attttgatga catgaaactt cctcatggat tctacgggg                  529


<210>  174
<211>  1029
<212>  DNA
<213>  artificial

<220>
<223>  (N23)aslA-HF1.0(N24)

<400>  174
ttcaaacaga aaggccatgg gccagtacga cgatcgccta ctgctgccga ttcctcgact       60

gaaaacaaac aatccggaac agctggaaaa agtgctgcgc cagcaaatca aaaacgtcgg      120

cgatcgcccg ctgttgtgga gcacactggg ccagtcactg atgaagcacg gagaatggca      180

ggaagcatcg ctcgccttcc gcgcagcgct gaaacaacgt ccggacgcct acgattacgc      240

atggcttgcc gacgcgctgg acagactgca caagccggaa gaagctgcag ctatgcgtcg      300

cgacggtttg atgttaacgt tgcagaataa cccgccacag tagttccttc tcacccggag      360

gcaagcacct ccggggcctt cctgatacat aaaaaaacgc ctgctcttat tacggagcag      420

gcgttaaaac aggtctgtat gacaacaagt gggtgcttca ctcaacgttg tgtccatggt      480

gtctgatgag gcataagcga catctgtcag tggacgataa gcaccgtaaa cggctctgcg      540

tcattccgga gtttatgagg cactaaggcg aacataagag atggaatgag catctactcg      600

tttattatgc cacagagaat cgggaaataa catcccttaa cacttgttat gagataattc      660

tgtaatcctc tttgcttcct gagtaataac ttcctgagtg aatatttaac ctgagcttga      720

tcctacacat acttattatg aatgataaaa ttcattcaat taataacaca tatattaatt      780

gccgttaaaa ctaaaaacag catcaataat caacgcgata taataaacct gccttacata      840

tcaactgcgc cagaggtagg attgaaaacg ctctcctgat tttccaattc attttctgga      900

taaataaata atttattttt gtcactatta tttatgtaat catcctgtca gggagaggga      960

tctcaattat caatgcttaa ttacgtcatc attttgatga catgaaactt cctcatggat     1020

tctacgggg                                                             1029


<210>  175
<211>  1035
<212>  DNA
<213>  artificial

<220>
<223>  (N23)melB-HF1.0(N24)

<400>  175
ttcaaacaga aaggccatgg gcccaatggc gatgaatacc tgggcgatgt atgcccgcta       60

tccgcatatc aaacaggtcg ggctgtgcca ttcggtgcag ggaacggcgg aagagttggc      120

gcgtgacctc aatatcgacc cagctacgct gcgttaccgt tgcgcaggta tcaaccatat      180

ggcgttttac ctggagctgg agcgcaaaac cgccgacggc agttatgtga atctctaccc      240

ggaactgctg gcggcttatg aagcagggca ggcaccgaag ccgaatattc atggcaatac      300

tcgctgccag aatattgtgc gctacgaaat gttcaaaaag ctgggctatt tcgtcacgga      360

atcgtcagaa cattttgctg agtacacacc gtggtttatt aagccaggtc gtgaggattt      420

gattgagcgt tataaagtac cgctggatga gtacccgaaa cgctgcgtcg agcagctggc      480

gaactggcat aaagagctgg aggagtataa aaaagcctcc cggattgata ttaaaccgtc      540

acgggaatat gccagcacaa tcatgaacgc tatctggact ggcgagccga gtgtgattta      600

cggcaacgtc cgtaacgatg gtttgattga taacctgcca caaggatgtt gcgtggaagt      660

agcctgtctg gttgatgcta atggcattca gccgaccaaa gtcggtacgc taccttcgca      720

tctggccgcc ctgatgcaaa ccaacatcaa cgtacagacg ctgctgaccg aagctattct      780

tacggaaaat cgcgaccgtg tttaccacgc cgcgatgatg gacccgcata ctgccgccgt      840

gctgggcatt gacgaaatat atgctcttgt tgacgacctg attgccgccc acggcgactg      900

gctgccaggc tggttgcacc gttaaaacgc gactaaacgc tactgcgccg ggggatttat      960

tccggcgcac acctctgacg ataccaataa cagaaggcgg gcgttggtaa cagcttcctc     1020

atggattcta cgggg                                                      1035


<210>  176
<211>  1189
<212>  DNA
<213>  artificial

<220>
<223>  (N23)rcsB-HF1.0(N24)

<400>  176
ttcaaacaga aaggccatgg ggttagcgaa catgcttgcg gatgatagct ggaaaagtga       60

gacggtgctg ttctccgtgc aggatttaat tgatgaagtt gtgccttcag tgttgcctgc      120

catcaagcgt aaaggtctgc aactgctgat taacaatcat ctgaaagcac acgatatgcg      180

ccgcggcgat cgcgatgcct tacgacgtat tttgctgcta ctgatgcaat atgccgtgac      240

ctcaacgcaa ttgggaaaaa tcacccttga ggttgatcag gatgagtcct ccgaagaccg      300

cctgacgttc cgcattctgg acacgggaga aggcgtaagt attcatgaaa tggataattt      360

gcacttcccg tttatcaacc agacccaaaa cgatcgctat ggcaaggcgg acccgctggc      420

attctggctg agcgatcaac tggcacgtaa actgggcggt catttaaaca tcaaaacgcg      480

ggatgggctt ggtacacgct actctgtgca tatcaaaatg ctcgcagctg acccggaagt      540

tgaagaggaa gaagagcgtt tactggatga tgtctgcgta atggtggatg ttacttcggc      600

agaaattcgg aatattgtca ctcgccagtt agaaaattgg ggtgcaacct gtatcacacc      660

cgatgaaaga ttaattagtc aagattatga tatcttttta acggataatc cgtctaatct      720

tactgcctct ggcttgcttt taagcgatga tgagtctggc gtacgggaaa ttgggcctgg      780

tcaattgtgc gtcaacttca atatgagcaa cgctatgcag gaagcggtct tacaattaat      840

tgaagtgcaa ctggcgcagg aagaggtgac agaatcgcct ctgggcggag atgaaaatgc      900

gcaactccat gccagcggct attatgcgct ctttgtagac acagtaccgg atgatgttaa      960

gaggctgtat actgaagcag caaccagtga ctttgctgcg ttagcccaaa cggctcatcg     1020

tcttaaaggc gtatttgcca tgctaaatct ggtacccggc aagcagttat gtgaaacgct     1080

ggaacatctg attcgtgaga aggatgttcc aggaatagaa aaatacatca gcgacattga     1140

cagttatgtc aagagcttgc tgtagcaatt cctcatggat tctacgggg                 1189


<210>  177
<211>  443
<212>  DNA
<213>  artificial

<220>
<223>  (N23)tyrR-HF0.5(N24)

<400>  177
cagcccgctg gcgttggtcg atatggcgtt tatcgcctgg cgcaatctgc gtttaattaa       60

tcgcatcgcc acgctgtatg gcattgaact ggggtattac agccgtttgc gtctgtttaa      120

gctggtattg ctgaatatcg cttttgccgg agccagcgaa ctggtgcgcg aagtggggat      180

ggactggatg tcgcaagatc tcgctgctcg tttgtctacc cgcgcagctc aggggattgg      240

tgcaggactt ctgacggcac gactcgggat taaagctatg gagctttgcc gcccgctgcc      300

gtggattgac gatgacaaac ctcgcctcgg ggatttccgt cgtcagctta tcggtcaggt      360

gaaagaaacg ctgcaaaaag gcaaaacgcc cagcgaaaaa taatgcaata tcgggtgctg      420

acttcctcat ggattctacg ggg                                              443


<210>  178
<211>  496
<212>  DNA
<213>  artificial

<220>
<223>  N23)pheA-HF0.5(N24)

<400>  178
ttcaaacaga aaggccatgg gcatgtcgca gaccgtctcg ccaaactgga aaaatggcaa       60

acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac      120

gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac      180

accgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa      240

ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa      300

gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg      360

acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca      420

cataccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgttcct      480

catggattct acgggg                                                      496


<210>  179
<211>  496
<212>  DNA
<213>  artificial

<220>
<223>  (N23)ptsI-HF0.7(N24)

<400>  179
ttcaaacaga aaggccatgg gcatgtcgca gaccgtctcg ccaaactgga aaaatggcaa       60

acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac      120

gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac      180

accgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa      240

ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa      300

gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg      360

acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca      420

cataccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgttcct      480

catggattct acgggg                                                      496


<210>  180
<211>  542
<212>  DNA
<213>  artificial

<220>
<223>  (N24)nupG-HT0.5(N21)

<400>  180
ttcctcatgg attctacggg gttacgcaaa gaaaaacggg tcgccagaag gtgacccgtt       60

ttttttattc ttacttcaac acataaccgt acaaccgttt cacgccatcc gcatcggttt      120

cgctataaac accttgcagc tccggcgaaa atcccggcaa caaattcacc ccttcttcca      180

gtgcaaggaa ataacgttga accgccccac cccagacttc cccgggtacc acgcaaagca      240

cgccaggtgg ataaggcaac gccccttctg ccgcaattcg cccttcggca tcacgaatcc      300

gcaccaactc cacgtcaccg cgaatataag cgctatgcgc atcctggggg ttcatcacca      360

ctgacgggaa actctgctgg cggaacatcg ctttttgtag gtctttgacg tcgaaactga      420

catacagatc gtgcatctcc tgacacaact ggcgcagggt gtagtcgcga tagcgcaccg      480

gatacttgtt ataaacgctc ggcaacacct caaccagcgg cttccctcga ctcacacttg      540

gg                                                                     542


<210>  181
<211>  1092
<212>  DNA
<213>  artificial

<220>
<223>  (N24)nupG-HT1.0(N21)

<400>  181
ttcctcatgg attctacggg gttacgcaaa gaaaaacggg tcgccagaag gtgacccgtt       60

ttttttattc ttacttcaac acataaccgt acaaccgttt cacgccatcc gcatcggttt      120

cgctataaac accttgcagc tccggcgaaa atcccggcaa caaattcacc ccttcttcca      180

gtgcaaggaa ataacgttga accgccccac cccagacttc cccgggtacc acgcaaagca      240

cgccaggtgg ataaggcaac gccccttctg ccgcaattcg cccttcggca tcacgaatcc      300

gcaccaactc cacgtcaccg cgaatataag cgctatgcgc atcctggggg ttcatcacca      360

ctgacgggaa actctgctgg cggaacatcg ctttttgtag gtctttgacg tcgaaactga      420

catacagatc gtgcatctcc tgacacaact ggcgcagggt gtagtcgcga tagcgcaccg      480

gatacttgtt ataaacgctc ggcaacacct caaccagcgg cgagtcatcc tcaatatgct      540

gttcaaattg cgccagcatc gccaccagtt gtgccagctt ctcgtggctt tccgccggag      600

ttaataaaaa cagaatggag ttgagatcgc acttctccgg cacaatgccg ttctcacgca      660

gatagtgcgc cagaatcgtc gccggaacgc caaagtcgct atattcgccg gtttcggcat      720

cgatacctgg tgtagtgagt aacagcttgc acggatcaac aaaatactga tccgcggcat      780

atccttcaaa gccgtgccac ttcgcccccg gctcaaaact gaaaaaacgg cggtcgctgg      840

ctaacactga tgtcggataa tcctgccaca atttgccatc aacaacgggc gggataaacg      900

ggcggaacag cttacagcgc gcaagaatag ccttgcgcgc ttcaatccct atctcaacac      960

actcagccca cagccgacgc ccactctccc cttcatgaat tttggcgtta acatccagtg     1020

cagcaaacag cggatagaaa gggctggtag aagcatggag cataaaggcg tttccctcga     1080

ctcacacttg gg                                                         1092


<210>  182
<211>  1543
<212>  DNA
<213>  artificial

<220>
<223>  (N24)nupG-HT1.5(N21)

<400>  182
ttcctcatgg attctacggg gttacgcaaa gaaaaacggg tcgccagaag gtgacccgtt       60

ttttttattc ttacttcaac acataaccgt acaaccgttt cacgccatcc gcatcggttt      120

cgctataaac accttgcagc tccggcgaaa atcccggcaa caaattcacc ccttcttcca      180

gtgcaaggaa ataacgttga accgccccac cccagacttc cccgggtacc acgcaaagca      240

cgccaggtgg ataaggcaac gccccttctg ccgcaattcg cccttcggca tcacgaatcc      300

gcaccaactc cacgtcaccg cgaatataag cgctatgcgc atcctggggg ttcatcacca      360

ctgacgggaa actctgctgg cggaacatcg ctttttgtag gtctttgacg tcgaaactga      420

catacagatc gtgcatctcc tgacacaact ggcgcagggt gtagtcgcga tagcgcaccg      480

gatacttgtt ataaacgctc ggcaacacct caaccagcgg cgagtcatcc tcaatatgct      540

gttcaaattg cgccagcatc gccaccagtt gtgccagctt ctcgtggctt tccgccggag      600

ttaataaaaa cagaatggag ttgagatcgc acttctccgg cacaatgccg ttctcacgca      660

gatagtgcgc cagaatcgtc gccggaacgc caaagtcgct atattcgccg gtttcggcat      720

cgatacctgg tgtagtgagt aacagcttgc acggatcaac aaaatactga tccgcggcat      780

atccttcaaa gccgtgccac ttcgcccccg gctcaaaact gaaaaaacgg cggtcgctgg      840

ctaacactga tgtcggataa tcctgccaca atttgccatc aacaacgggc gggataaacg      900

ggcggaacag cttacagcgc gcaagaatag ccttgcgcgc ttcaatccct atctcaacac      960

actcagccca cagccgacgc ccactctccc cttcatgaat tttggcgtta acatccagtg     1020

cagcaaacag cggatagaaa gggctggtag aagcatggag cataaaggcg ttattcaacc     1080

gcttatgcgg gcaaaaacgc gcctgtccgc ggatatggtt atctttttta tggatctgcg     1140

acgtctgtga gaatcccgcc tgctgtttgt gcaccgactg agtcacaaag atccccggat     1200

cgttttcgtt aagttctaac agcagcggcg agctatccgc catcatcggg ataaattgtt     1260

cataaccgac ccacgcggaa tcaaacagaa tgtaatcaca cagatgccca acggtatcga     1320

tcacctgacg ggcgttatag acagtgccgt cataggttcc cagctgaata atcgccaggc     1380

gatacgggcg cggcaggtcg gctttttctg gcgcaacgtc gcgaatttgc tggcgcagat     1440

actcttcatt aaaacagtgc gcatcaatac cgccaatgaa accaaacggg ttgcgtgaag     1500

cttccagata gaccggcgtc gcttccctcg actcacactt ggg                       1543


<210>  183
<211>  542
<212>  DNA
<213>  artificial

<220>
<223>  (N24)aslA-HT0.5(N21)

<400>  183
ttcctcatgg attctacggg ggccggcgct atcgctgaga tctgcctttg ccggatgcga       60

tgctgacgca tcttatccag cctacagaac gctgcaattt attgaatttg cacgatcatg      120

taggccggat aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc cgccgatttc      180

tacttactca ccaccagcaa atgcgcatgc ataatgtcgc tggccgggcg accgtgcgcc      240

agcaaatcag ccattgcttt aagatatggc ggtagatggc ggaaataacg ctgatacccg      300

gcacacaaat aattcagtcc cggtttgccg ctggcatcga gcatgaagcg gtgtttcggg      360

cagcctcccc agcacgcttt taacacgtta caactgcgac actgcgccgg taactgctta      420

aatttatctt caccaaacgc ctgctgttgc ggggaatcga tcatttctgc aattgtttgc      480

tggtgcatat tccccagccg atattgcgga taaacatagt gttccctcga ctcacacttg      540

gg                                                                     542


<210>  184
<211>  1042
<212>  DNA
<213>  artificial

<220>
<223>  (N24)aslA-HT1.0(N21)

<400>  184
ttcctcatgg attctacggg gcggtaaact cgctgctgtg cgtatggatg agttcaagta       60

tcacgtcctg attcagcaac cttacgctta tacccagagc ggatatcagg gtggattcac      120

cggcacagta atgcaaacgg cgggatcgtc ggtgtttaac ctctacaccg atccgcagga      180

aagcgactcc atcggcgtgc gccatattcc gatgggtgta ccgctacaga ccgaaatgca      240

cgcgtatatg gagatcctga aaaaatatcc accacgcgcg cagattaaat ctgactaagc      300

cggcgctatc gctgagatct gcctttgccg gatgcgatgc tgacgcatct tatccagcct      360

acagaacgct gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc      420

cgcatccggc aatcaaccgc aggcggccgc cgatttctac ttactcacca ccagcaaatg      480

cgcatgcata atgtcgctgg ccgggcgacc gtgcgccagc aaatcagcca ttgctttaag      540

atatggcggt agatggcgga aataacgctg atacccggca cacaaataat tcagtcccgg      600

tttgccgctg gcatcgagca tgaagcggtg tttcgggcag cctccccagc acgcttttaa      660

cacgttacaa ctgcgacact gcgccggtaa ctgcttaaat ttatcttcac caaacgcctg      720

ctgttgcggg gaatcgatca tttctgcaat tgtttgctgg tgcatattcc ccagccgata      780

ttgcggataa acatagtgat cacaggcgta aacgtcgccg ttgtgctcaa caatcaccga      840

gcgcccacag gttggctgat gatggcaaac cgcacccggc gcaccgacaa aattggcaaa      900

cgcccattcg atattcatca cgaaaatctt gccgacgtcg cgtttgatcc agtggtcgaa      960

tatcgccacc agaaactcac cgaactcctc ggggcgcacc gaccattccg ttagctcacc     1020

cttccctcga ctcacacttg gg                                              1042


<210>  185
<211>  1543
<212>  DNA
<213>  artificial

<220>
<223>  (N24)melB-HT1.0(N21)

<400>  185
ttcctcatgg attctacggg gttacgcaaa gaaaaacggg tcgccagaag gtgacccgtt       60

ttttttattc ttacttcaac acataaccgt acaaccgttt cacgccatcc gcatcggttt      120

cgctataaac accttgcagc tccggcgaaa atcccggcaa caaattcacc ccttcttcca      180

gtgcaaggaa ataacgttga accgccccac cccagacttc cccgggtacc acgcaaagca      240

cgccaggtgg ataaggcaac gccccttctg ccgcaattcg cccttcggca tcacgaatcc      300

gcaccaactc cacgtcaccg cgaatataag cgctatgcgc atcctggggg ttcatcacca      360

ctgacgggaa actctgctgg cggaacatcg ctttttgtag gtctttgacg tcgaaactga      420

catacagatc gtgcatctcc tgacacaact ggcgcagggt gtagtcgcga tagcgcaccg      480

gatacttgtt ataaacgctc ggcaacacct caaccagcgg cgagtcatcc tcaatatgct      540

gttcaaattg cgccagcatc gccaccagtt gtgccagctt ctcgtggctt tccgccggag      600

ttaataaaaa cagaatggag ttgagatcgc acttctccgg cacaatgccg ttctcacgca      660

gatagtgcgc cagaatcgtc gccggaacgc caaagtcgct atattcgccg gtttcggcat      720

cgatacctgg tgtagtgagt aacagcttgc acggatcaac aaaatactga tccgcggcat      780

atccttcaaa gccgtgccac ttcgcccccg gctcaaaact gaaaaaacgg cggtcgctgg      840

ctaacactga tgtcggataa tcctgccaca atttgccatc aacaacgggc gggataaacg      900

ggcggaacag cttacagcgc gcaagaatag ccttgcgcgc ttcaatccct atctcaacac      960

actcagccca cagccgacgc ccactctccc cttcatgaat tttggcgtta acatccagtg     1020

cagcaaacag cggatagaaa gggctggtag aagcatggag cataaaggcg ttattcaacc     1080

gcttatgcgg gcaaaaacgc gcctgtccgc ggatatggtt atctttttta tggatctgcg     1140

acgtctgtga gaatcccgcc tgctgtttgt gcaccgactg agtcacaaag atccccggat     1200

cgttttcgtt aagttctaac agcagcggcg agctatccgc catcatcggg ataaattgtt     1260

cataaccgac ccacgcggaa tcaaacagaa tgtaatcaca cagatgccca acggtatcga     1320

tcacctgacg ggcgttatag acagtgccgt cataggttcc cagctgaata atcgccaggc     1380

gatacgggcg cggcaggtcg gctttttctg gcgcaacgtc gcgaatttgc tggcgcagat     1440

actcttcatt aaaacagtgc gcatcaatac cgccaatgaa accaaacggg ttgcgtgaag     1500

cttccagata gaccggcgtc gcttccctcg actcacactt ggg                       1543


<210>  186
<211>  542
<212>  DNA
<213>  artificial

<220>
<223>  (N24)rcsB-HT1.0(N21)

<400>  186
ttcctcatgg attctacggg ggccggcgct atcgctgaga tctgcctttg ccggatgcga       60

tgctgacgca tcttatccag cctacagaac gctgcaattt attgaatttg cacgatcatg      120

taggccggat aaggcgttta cgccgcatcc ggcaatcaac cgcaggcggc cgccgatttc      180

tacttactca ccaccagcaa atgcgcatgc ataatgtcgc tggccgggcg accgtgcgcc      240

agcaaatcag ccattgcttt aagatatggc ggtagatggc ggaaataacg ctgatacccg      300

gcacacaaat aattcagtcc cggtttgccg ctggcatcga gcatgaagcg gtgtttcggg      360

cagcctcccc agcacgcttt taacacgtta caactgcgac actgcgccgg taactgctta      420

aatttatctt caccaaacgc ctgctgttgc ggggaatcga tcatttctgc aattgtttgc      480

tggtgcatat tccccagccg atattgcgga taaacatagt gttccctcga ctcacacttg      540

gg                                                                     542


<210>  187
<211>  549
<212>  DNA
<213>  artificial

<220>
<223>  (N24)tyrR-HT0.5(N21)

<400>  187
ttcctcatgg attctacggg gttatgcttt cagtacagcc agagctgctt cgtaatccgg       60

ctcggtggtg atttcatcca ccagctggct gaaaatcaca ttgtcatttt cgtcaataac      120

cacaacggca cgcgctgcca gacctttcag tgggccatca gcaattgcca caccgtaagc      180

ttgcagaaat tcagcgttac ggaaagtgga gagggtgata acgttgttca gaccttctgc      240

gccgcagaaa cgagactggg cgaacggcag atcggcagag atacacagca caacggtgtt      300

gtcgatctca gttgccagtt ggttaaactt acgtactgat gcggcgcaaa caccggtatc      360

aatactcggg aaaatgttca gcactttgcg tttacccgca aactgaccga gggtgacgtc      420

agacagatct tttgccacga gagtaaaagt ctgcgctttg ctacccgcct gcgggatgga      480

attggcgact gtaaccgggt tgccctggaa atgaacggtt tgtgacattt ccctcgactc      540

acacttggg                                                              549


<210>  188
<211>  631
<212>  DNA
<213>  artificial

<220>
<223>  (N24)pheA-HT0.5(N21)

<400>  188
ttcctcatgg attctacggg gttactggcg attgtcattc gcctgacgca ataacacgcg       60

gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact      120

gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt      180

atagtaacgt ttgattaacg ccagattacg ctctgacgac ataatgatgt cggcataaag      240

ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga      300

agagagcgcc agaagttgct caagctgaac attttcttct gccaggtgca gcccgtaagc      360

aaaagtagca aagtggcgca gtgcctgaat aaacgccata ttctgatcgt gctcgacggc      420

gctaatacga tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc      480

ttccggttta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg      540

accgaacatc gggtgtagcc ccagcaccgg accatcatgc gccaccagca tggcctgtaa      600

tggcccattt ttccctcgac tcacacttgg g                                     631


<210>  189
<211>  818
<212>  DNA
<213>  artificial

<220>
<223>  (N24)ptsI-HT0.7(N21)

<400>  189
ttcctcatgg attctacggg gagcgcatca cttccagtac gcgcaacccc gctcggtgca       60

ctgcatcggt taacgccttc cctttcagca agccactgat gagctgagca caaaacaggt      120

cgccagtccc tttcaggtcg gtttttaccc gtgaatggga aatgacattc acgctgtcgg      180

cagtgaccac cacaacctgc atctcctgat tttcttcatt accggaggcg ctggtaacca      240

ccacccattt taatgtgtct gaaagcagac tttttgcggc agcaatggca ctgtcgagat      300

cgcggcaatt tttaccggtc aggatttcca actcaaagat attgggggta attccctgcg      360

ccagcggcag taaatattgt cgatacgctt cgggaaggtc aggtttgaca taaattccgc      420

tatcaatatc gccaatcacc ggatcgacca tgatcaatag gtcaggatgg tctttgcgta      480

gcgcagtcag ccactcggca aggattttga tttgcgatgc cgttcccata tagcccgtgg      540

ttacagcacg aagttggcgc agcgcatcac gctcctgaag cgcacgcaaa tagccgctaa      600

accattcgtc cggaatcgca ccaccgtaga aagtgtcata atgcggcgta ttgctcagca      660

ataccgtcgg cacggcaaag acattcaggc cgttctgttt gatagcaggc acggcaatgc      720

tgttgcccac gctgccgtaa accacctgcg actgcacggc gacgatatcc gcctgcagtg      780

ccctactctt atcgttattc cctcgactca cacttggg                              818


<210>  190
<211>  1119
<212>  DNA
<213>  artificial

<220>
<223>  (N21)aadA(N22)

<400>  190
ttccctcgac tcacacttgg gtcgacctgc agaagcttag atctattacc ctgttatccc       60

tactcgagtt catgtgcagc tccatcagca aaaggggatg ataagtttat caccaccgac      120

tatttgcaac agtgccgttg atcgtgctat gatcgactga tgtcatcagc ggtggagtgc      180

aatgtcatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc      240

gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg      300

gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt      360

gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga      420

gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg      480

tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt      540

gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca      600

agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct      660

gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac      720

tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta      780

accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc      840

cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc      900

ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc      960

aaggtagtcg gcaaataaga tgccgctcgc cagtcgattg gctgagctca tgaagttcct     1020

attccgaagt tccgcgaacg cgtaaaggat ctaggtgaag atcctttttg ataatctcat     1080

gaccaaaatc ccttaacgtt cacacaacat agccacggg                            1119


<210>  191
<211>  1125
<212>  DNA
<213>  artificial

<220>
<223>  (N21)bla(N22)

<400>  191
ttccctcgac tcacacttgg gtttctacaa actcttttgt ttatttttct aaatacattc       60

aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag      120

gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg      180

ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt      240

gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt      300

tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt      360

attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa      420

tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag      480

agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac      540

aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac      600

tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac      660

cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac      720

tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact      780

tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg      840

tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt      900

tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat      960

aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta     1020

gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa     1080

tctcatgacc aaaatccctt aacgttcaca caacatagcc acggg                     1125


<210>  192
<211>  1414
<212>  DNA
<213>  artificial

<220>
<223>  (N22)p5(N23)

<400>  192
ttcacacaac atagccacgg gccgttttca tctgtgcata tggacagttt tccctttgat       60

atgtaacggt gaacagttgt tctacttttg tttgttagtc ttgatgcttc actgatagat      120

acaagagcca taagaacctc agatccttcc gtatttagcc agtatgttct ctagtgtggt      180

tcgttgtttt tgcgtgagcc atgagaacga accattgaga tcatacttac tttgcatgtc      240

actcaaaaat tttgcctcaa aactggtgag ctgaattttt gcagttaaag catcgtgtag      300

tgtttttctt agtccgttat gtaggtagga atctgatgta atggttgttg gtattttgtc      360

accattcatt tttatctggt tgttctcaag ttcggttacg agatccattt gtctatctag      420

ttcaacttgg aaaatcaacg tatcagtcgg gcggcctcgc ttatcaacca ccaatttcat      480

attgctgtaa gtgtttaaat ctttacttat tggtttcaaa acccattggt taagcctttt      540

aaactcatgg tagttatttt caagcattaa catgaactta aattcatcaa ggctaatctc      600

tatatttgcc ttgtgagttt tcttttgtgt tagttctttt aataaccact cataaatcct      660

catagagtat ttgttttcaa aagacttaac atgttccaga ttatatttta tgaatttttt      720

taactggaaa agataaggca atatctcttc actaaaaact aattctaatt tttcgcttga      780

gaacttggca tagtttgtcc actggaaaat ctcaaagcct ttaaccaaag gattcctgat      840

ttccacagtt ctcgtcatca gctctctggt tgctttagct aatacaccat aagcattttc      900

cctactgatg ttcatcatct gagcgtattg gttataagtg aacgataccg tccgttcttt      960

ccttgtaggg ttttcaatcg tggggttgag tagtgccaca cagcataaaa ttagcttggt     1020

ttcatgctcc gttaagtcat agcgactaat cgctagttca tttgctttga aaacaactaa     1080

ttcagacata catctcaatt ggtctaggtg attttaatca ctataccaat tgagatgggc     1140

tagtcaatga taattactag tccttttcct ttgagttgtg ggtatctgta aattctgcta     1200

gacctttgct ggaaaacttg taaattctgc tagaccctct gtaaattccg ctagaccttt     1260

gtgtgttttt tttgtttata ttcaagtggt tataatttat agaataaaga aagaataaaa     1320

aaagataaaa agaatagatc ccagccctgt gtataactca ctactttagt cagttccgca     1380

gtattacaaa aggattcaaa cagaaaggcc atgg                                 1414


<210>  193
<211>  1130
<212>  DNA
<213>  artificial

<220>
<223>  (N22)p15(N23)

<400>  193
ttcacacaac atagccacgg gtgttcagct actgacgggg tggtgcgtaa cggcaaaagc       60

accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc actgatgagg      120

gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc gtcagcagaa      180

tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg      240

actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag atgccaggaa      300

gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag gctccgcccc      360

cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta      420

taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt      480

cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact      540

cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc      600

cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa      660

agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg      720

ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac      780

ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt      840

tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta      900

ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg      960

aagtcagccc catacgatat aagttgtaat tctcatgttt gacagcttat cacccagtcc     1020

tgctcgcttc gctacttggc catacccacg ccgaaacaag cgctcatgag cccgaagtgg     1080

cgagcccgat cttccccatc ggtgatgtct tcaaacagaa aggccatggg                1130


<210>  194
<211>  943
<212>  DNA
<213>  artificial

<220>
<223>  (N22)pMB1(N23)

<400>  194
ttcacacaac atagccacgg gagttttcgt tccactgagc gtcagacccc gtagaaaaga       60

tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa      120

aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga      180

aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt      240

taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt      300

taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat      360

agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct      420

tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca      480

cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag      540

agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc      600

gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga      660

aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca      720

tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag      780

ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg      840

aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat      900

gctggatcct tgacagctag ctcagtccta ggtataatac tag                        943


<210>  195
<211>  857
<212>  DNA
<213>  artificial

<220>
<223>  (N22)pMB1 mutant(N23)

<400>  195
ttcacacaac atagccacgg ggctcactca aaggcggtaa tacggttatc cacagaatca       60

ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa      120

aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat      180

cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc      240

cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc      300

gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt      360

tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac      420

cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg      480

ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca      540

gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc      600

gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa      660

accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa      720

ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac      780

tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttca      840

aacagaaagg ccatggg                                                     857


<210>  196
<211>  163
<212>  DNA
<213>  artificial

<220>
<223>  (N23)pLac(RBS1)

<400>  196
ttcaaacaga aaggccatgg gcaacgcaat taatgtgagt tagctcactc attaggcacc       60

ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca      120

atagaaataa ttttgtttaa ctttaagaag gagatataca tat                        163


<210>  197
<211>  1780
<212>  DNA
<213>  artificial

<220>
<223>  N23)LacIT7(RBS1)

<400>  197
tcaaacagaa aggccatggg gaaactaccc ataatacaag aaaagcccgt cacgggcttc       60

tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc tgttcagcag      120

ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca ggtcattcag      180

actggctaat gcacccagta aggcagcggt atcatcaaca ggcttacccg tcttactgtc      240

gggaattcgc gttggccgat tcattaatgc agctggcacg acaggtttcc tctagatttc      300

agtgcaattt atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta      360

attctcatgt tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc      420

aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg      480

cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc      540

caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag      600

tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg      660

gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat      720

ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatgtccg caccaacgcg      780

cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag      840

catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat      900

ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt      960

atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc     1020

gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg     1080

ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac     1140

attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat     1200

cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc     1260

gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat     1320

cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag     1380

caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc     1440

catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac     1500

gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg     1560

tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa     1620

ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca     1680

ttaggaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag     1740

aaataatttt gtttaacttt aagaaggaga tatacatatg                           1780


<210>  198
<211>  242
<212>  DNA
<213>  artificial

<220>
<223>  (N31)t7t(N21)

<400>  198
tgatgggctg aagggtttaa gggctgctaa caaagcccga aaggaagctg agttggctgc       60

tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg      120

ttttttgctg aaaggaggaa ctatatccgg atatcccgca agaggcccgg cagtaccggc      180

ataaccaagc ctatgcctac agcatccagg gtgacggtgc cttccctcga ctcacacttg      240

gg                                                                     242


<210>  199
<211>  242
<212>  DNA
<213>  artificial

<220>
<223>  (N31)t7t(LK3)

<400>  199
tgatgggctg aagggtttaa gggctgctaa caaagcccga aaggaagctg agttggctgc       60

tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg      120

ttttttgctg aaaggaggaa ctatatccgg atatcccgca agaggcccgg cagtaccggc      180

ataaccaagc ctatgcctac agcatccagg gtgacggtgc ctcaggctcg tcttcttcag      240

gg                                                                     242


<210>  200
<211>  1671
<212>  DNA
<213>  artificial

<220>
<223>  (LK3)LacI(N21)

<400>  200
tcaggctcgt cttcttcagg ggaaactacc cataatacaa gaaaagcccg tcacgggctt       60

ctcagggcgt tttatggcgg gtctgctatg tggtgctatc tgactttttg ctgttcagca      120

gttcctgccc tctgattttc cagtctgacc acttcggatt atcccgtgac aggtcattca      180

gactggctaa tgcacccagt aaggcagcgg tatcatcaac aggcttaccc gtcttactgt      240

cgggaattcg cgttggccga ttcattaatg cagctggcac gacaggtttc ctctagattt      300

cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga tataagttgt      360

aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct      420

caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt      480

gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg      540

ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca      600

gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc      660

ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga      720

tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc      780

gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca      840

gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca      900

tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt      960

tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg     1020

cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat     1080

gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa     1140

cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga     1200

tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc     1260

cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa     1320

tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca     1380

gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg     1440

ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca     1500

cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg     1560

gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa     1620

aggttttgcg ccattcgatg gtgtccggga ttccctcgac tcacacttgg g              1671


<210>  201
<211>  1935
<212>  DNA
<213>  artificial

<220>
<223>  (RBS1)dxs(RBS2stop)

<400>  201
tagaaataat tttgtttaac tttaagaagg agatatacat atgagttttg atattgccaa       60

atacccgacc ctggcactgg tcgactccac ccaggagtta cgactgttgc cgaaagagag      120

tttaccgaaa ctctgcgacg aactgcgccg ctatttactc gacagcgtga gccgttccag      180

cgggcacttc gcctccgggc tgggcacggt cgaactgacc gtggcgctgc actatgtcta      240

caacaccccg tttgaccaat tgatttggga tgtggggcat caggcttatc cgcataaaat      300

tttgaccgga cgccgcgaca aaatcggcac catccgtcag aaaggcggtc tgcacccgtt      360

cccgtggcgc ggcgaaagcg aatatgacgt attaagcgtc gggcattcat caacctccat      420

cagtgccgga attggtattg cggttgctgc cgaaaaagaa ggcaaaaatc gccgcaccgt      480

ctgtgtcatt ggcgatggcg cgattaccgc aggcatggcg tttgaagcga tgaatcacgc      540

gggcgatatc cgtcctgata tgctggtgat tctcaacgac aatgaaatgt cgatttccga      600

aaatgtcggc gcgctcaaca accatctggc acagctgctt tccggtaagc tttactcttc      660

actgcgcgaa ggcgggaaaa aagttttctc tggcgtgccg ccaattaaag agctgctcaa      720

acgcaccgaa gaacatatta aaggcatggt agtgcctggc acgttgtttg aagagctggg      780

ctttaactac atcggcccgg tggacggtca cgatgtgctg gggcttatca ccacgctaaa      840

gaacatgcgc gacctgaaag gcccgcagtt cctgcatatc atgaccaaaa aaggtcgtgg      900

ttatgaaccg gcagaaaaag acccgatcac tttccacgcc gtgcctaaat ttgatccctc      960

cagcggttgt ttgccgaaaa gtagcggcgg tttgccgagc tattcaaaaa tctttggcga     1020

ctggttgtgc gaaacggcag cgaaagacaa caagctgatg gcgattactc cggcgatgcg     1080

tgaaggttcc ggcatggtcg agttttcacg taaattcccg gatcgctact tcgacgtggc     1140

aattgccgag caacacgcgg tgacctttgc tgcgggtctg gcgattggtg ggtacaaacc     1200

cattgtcgcg atttactcca ctttcctgca acgcgcctat gatcaggtgc tgcatgacgt     1260

ggcgattcaa aagcttccgg tcctgttcgc catcgaccgc gcgggcattg ttggtgctga     1320

cggtcaaacc catcagggtg cttttgatct ctcttacctg cgctgcatac cggaaatggt     1380

cattatgacc ccgagcgatg aaaacgaatg tcgccagatg ctctataccg gctatcacta     1440

taacgatggc ccgtcagcgg tgcgctaccc gcgtggcaac gcggtcggcg tggaactgac     1500

gccgctggaa aaactaccaa ttggcaaagg cattgtgaag cgtcgtggcg agaaactggc     1560

gatccttaac tttggtacgc tgatgccaga agcggcgaaa gtcgccgaat cgctgaacgc     1620

cacgctggtc gatatgcgtt ttgtgaaacc gcttgatgaa gcgttaattc tggaaatggc     1680

cgccagccat gaagcgctgg tcaccgtaga agaaaacgcc attatgggcg gcgcaggcag     1740

cggcgtgaac gaagtgctga tggcccatcg taaaccagta cccgtgctga acattggcct     1800

gccggacttc tttattccgc aaggaactca ggaagaaatg cgcgccgaac tcggcctcga     1860

tgccgctggt atggaagcca aaatcaaggc ctggctggca taaccgttca tttatcacaa     1920

aaggattgtt cgatg                                                      1935


<210>  202
<211>  602
<212>  DNA
<213>  artificial

<220>
<223>  (RBS2stop)idi(RBS3stop)

<400>  202
taaccgttca tttatcacaa aaggattgtt cgatgcaaac ggaacacgtc attttattga       60

atgcacaggg agttcccacg ggtacgctgg aaaagtatgc cgcacacacg gcagacaccc      120

gcttacatct cgcgttctcc agttggctgt ttaatgccaa aggacaatta ttagttaccc      180

gccgcgcact gagcaaaaaa gcatggcctg gcgtgtggac taactcggtt tgtgggcacc      240

cacaactggg agaaagcaac gaagacgcag tgatccgccg ttgccgttat gagcttggcg      300

tggaaattac gcctcctgaa tctatctatc ctgactttcg ctaccgcgcc accgatccga      360

gtggcattgt ggaaaatgaa gtgtgtccgg tatttgccgc acgcaccact agtgcgttac      420

agatcaatga tgatgaagtg atggattatc aatggtgtga tttagcagat gtattacacg      480

gtattgatgc cacgccgtgg gcgttcagtc cgtggatggt gatgcaggcg acaaatcgcg      540

aagccagaaa acgattatct gcatttaccc agcttaaatg attcacacag gaaacagcta      600

tg                                                                     602


<210>  203
<211>  1826
<212>  DNA
<213>  artificial

<220>
<223>  (RBS3stop)valC(RBS4stop)

<400>  203
tgattcacac aggaaacagc tatggccgag atgttcaacg gcaactcttc taacgacgga       60

tcttcttgca tgcccgtgaa ggacgccctg cgacgaaccg gcaaccacca ccccaacctg      120

tggaccgacg acttcatcca gtctctgaac tctccctact ctgactcttc ttaccacaag      180

caccgagaga tcctgatcga cgagatccga gacatgttct ctaacggcga gggcgacgag      240

ttcggcgtgc tcgagaacat ctggttcgtg gacgtggtgc agcgactggg catcgaccga      300

cacttccagg aggagatcaa gaccgccctg gactacatct acaagttctg gaaccacgac      360

tctatcttcg gcgacctgaa catggtggcc ctgggcttcc gaatcctgcg actgaaccga      420

tacgtggcct cttctgacgt gttcaagaag ttcaagggcg aggagggcca gttctctggc      480

ttcgagtcct ctgaccagga cgctaagctc gaaatgatgc tgaacctgta caaggcctct      540

gagctggact tccccgacga ggacatcctg aaggaggccc gagccttcgc ctctatgtac      600

ctgaagcacg tgatcaagga gtacggcgac atccaggagt ctaagaaccc cctgctgatg      660

gagatcgagt acaccttcaa gtacccctgg cgatgccgac tgccccgact cgaggcctgg      720

aacttcatcc acatcatgcg acagcaggac tgcaacatct ctctggccaa caacctctac      780

aagatcccca agatctacat gaagaagatc ctcgagctgg ccatcctgga cttcaacatc      840

ctgcagtctc agcaccagca cgagatgaag ctgatctcta cctggtggaa gaactcttct      900

gctatccagc tggacttctt ccgacaccga cacatcgagt cttacttttg gtgggcctcg      960

cccctgttcg agcccgagtt ctctacctgc cgaatcaact gcaccaagct gtctaccaag     1020

atgttcctgc tggacgacat ctacgacacc tacggcaccg tcgaggagct gaagcccttc     1080

accaccaccc tgacccgatg ggacgtgtct accgtggaca accaccccga ctacatgaag     1140

atcgccttca acttctctta cgagatctac aaggagatcg cctctgaggc cgagcgaaag     1200

cacggcccct tcgtgtacaa gtacctgcag tcttgctgga agtcttacat cgaggcctac     1260

atgcaggagg ccgagtggat cgcctctaac cacatccccg gcttcgacga gtacctgatg     1320

aacggcgtga agtcctctgg catgcgaatc ctgatgatcc acgccctgat cctgatggac     1380

acccccctgt ctgacgagat tctcgagcag ctggacatcc cctcgtctaa gtctcaggcc     1440

ctgctgtctc tgatcacccg actggtggac gacgtgaagg acttcgagga cgagcaggcc     1500

cacggcgaga tggcctcttc tatcgagtgc tacatgaagg acaaccacgg ctctacccga     1560

gaggacgccc tgaactacct gaagatccga atcgagtctt gcgtgcagga gctgaacaag     1620

gagctgctcg agccctctaa catgcacgga tctttccgaa acctgtacct gaacgtggga     1680

atgcgagtga ttttcttcat gctgaacgac ggcgacctgt tcacccactc taaccgaaag     1740

gagatccagg acgccatcac caagttcttc gtcgagccca tcatccccta aattaattgt     1800

tcttttttca ggtgaaggtt cccatg                                          1826


<210>  204
<211>  953
<212>  DNA
<213>  artificial

<220>
<223>  (RBS4stop)ispA(N31)

<400>  204
taaattaatt gttctttttt caggtgaagg ttcccatgga ctttccgcag caactcgaag       60

cctgcgttaa gcaggccaac caggcgctga gccgttttat cgccccactg ccctttcaga      120

acactcccgt ggtcgaaacc atgcagtatg gcgcattatt aggtggtaag cgcctgcgac      180

ctttcctggt ttatgccacc ggtcatatgt tcggcgttag cacaaacacg ctggacgcac      240

ccgctgccgc cgttgagtgt atccacgctt actcattaat tcatgatgat ttaccggcaa      300

tggatgatga cgatctgcgt cgcggtttgc caacctgcca tgtgaagttt ggcgaagcaa      360

acgcgattct cgctggcgac gctttacaaa cgctggcgtt ctcgatttta agcgatgccg      420

atatgccgga agtgtcggac cgcgacagaa tttcgatgat ttctgaactg gcgagcgcca      480

gtggtattgc cggaatgtgc ggtggtcagg cattagattt agacgcggaa ggcaaacacg      540

tacctctgga cgcgcttgag cgtattcatc gtcataaaac cggcgcattg attcgcgccg      600

ccgttcgcct tggtgcatta agcgccggag ataaaggacg tcgtgctctg ccggtactcg      660

acaagtatgc agagagcatc ggccttgcct tccaggttca ggatgacatc ctggatgtgg      720

tgggagatac tgcaacgttg ggaaaacgcc agggtgccga ccagcaactt ggtaaaagta      780

cctaccctgc acttctgggt cttgagcaag cccggaagaa agcccgggat ctgatcgacg      840

atgcccgtca gtcgctgaaa caactggctg aacagtcact cgatacctcg gcactggaag      900

cgctagcgga ctacatcatc cagcgtaata aatgatgggc tgaagggttt aag             953


<210>  205
<211>  621
<212>  DNA
<213>  artificial

<220>
<223>  (RBS1)idi(RBS2stop)

<400>  205
tagaaataat tttgtttaac tttaagaagg agatatacat atgcaaacgg aacacgtcat       60

tttattgaat gcacagggag ttcccacggg tacgctggaa aagtatgccg cacacacggc      120

agacacccgc ttacatctcg cgttctccag ttggctgttt aatgccaaag gacaattatt      180

agttacccgc cgcgcactga gcaaaaaagc atggcctggc gtgtggacta actcggtttg      240

tgggcaccca caactgggag aaagcaacga agacgcagtg atccgccgtt gccgttatga      300

gcttggcgtg gaaattacgc ctcctgaatc tatctatcct gactttcgct accgcgccac      360

cgatccgagt ggcattgtgg aaaatgaagt gtgtccggta tttgccgcac gcaccactag      420

tgcgttacag atcaatgatg atgaagtgat ggattatcaa tggtgtgatt tagcagatgt      480

attacacggt attgatgcca cgccgtgggc gttcagtccg tggatggtga tgcaggcgac      540

aaatcgcgaa gccagaaaac gattatctgc atttacccag cttaaataac cgttcattta      600

tcacaaaagg attgttcgat g                                                621


<210>  206
<211>  1916
<212>  DNA
<213>  artificial

<220>
<223>  (RBS2stop)dxs(RBS3stop)

<400>  206
taaccgttca tttatcacaa aaggattgtt cgatgagttt tgatattgcc aaatacccga       60

ccctggcact ggtcgactcc acccaggagt tacgactgtt gccgaaagag agtttaccga      120

aactctgcga cgaactgcgc cgctatttac tcgacagcgt gagccgttcc agcgggcact      180

tcgcctccgg gctgggcacg gtcgaactga ccgtggcgct gcactatgtc tacaacaccc      240

cgtttgacca attgatttgg gatgtggggc atcaggctta tccgcataaa attttgaccg      300

gacgccgcga caaaatcggc accatccgtc agaaaggcgg tctgcacccg ttcccgtggc      360

gcggcgaaag cgaatatgac gtattaagcg tcgggcattc atcaacctcc atcagtgccg      420

gaattggtat tgcggttgct gccgaaaaag aaggcaaaaa tcgccgcacc gtctgtgtca      480

ttggcgatgg cgcgattacc gcaggcatgg cgtttgaagc gatgaatcac gcgggcgata      540

tccgtcctga tatgctggtg attctcaacg acaatgaaat gtcgatttcc gaaaatgtcg      600

gcgcgctcaa caaccatctg gcacagctgc tttccggtaa gctttactct tcactgcgcg      660

aaggcgggaa aaaagttttc tctggcgtgc cgccaattaa agagctgctc aaacgcaccg      720

aagaacatat taaaggcatg gtagtgcctg gcacgttgtt tgaagagctg ggctttaact      780

acatcggccc ggtggacggt cacgatgtgc tggggcttat caccacgcta aagaacatgc      840

gcgacctgaa aggcccgcag ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac      900

cggcagaaaa agacccgatc actttccacg ccgtgcctaa atttgatccc tccagcggtt      960

gtttgccgaa aagtagcggc ggtttgccga gctattcaaa aatctttggc gactggttgt     1020

gcgaaacggc agcgaaagac aacaagctga tggcgattac tccggcgatg cgtgaaggtt     1080

ccggcatggt cgagttttca cgtaaattcc cggatcgcta cttcgacgtg gcaattgccg     1140

agcaacacgc ggtgaccttt gctgcgggtc tggcgattgg tgggtacaaa cccattgtcg     1200

cgatttactc cactttcctg caacgcgcct atgatcaggt gctgcatgac gtggcgattc     1260

aaaagcttcc ggtcctgttc gccatcgacc gcgcgggcat tgttggtgct gacggtcaaa     1320

cccatcaggg tgcttttgat ctctcttacc tgcgctgcat accggaaatg gtcattatga     1380

ccccgagcga tgaaaacgaa tgtcgccaga tgctctatac cggctatcac tataacgatg     1440

gcccgtcagc ggtgcgctac ccgcgtggca acgcggtcgg cgtggaactg acgccgctgg     1500

aaaaactacc aattggcaaa ggcattgtga agcgtcgtgg cgagaaactg gcgatcctta     1560

actttggtac gctgatgcca gaagcggcga aagtcgccga atcgctgaac gccacgctgg     1620

tcgatatgcg ttttgtgaaa ccgcttgatg aagcgttaat tctggaaatg gccgccagcc     1680

atgaagcgct ggtcaccgta gaagaaaacg ccattatggg cggcgcaggc agcggcgtga     1740

acgaagtgct gatggcccat cgtaaaccag tacccgtgct gaacattggc ctgccggact     1800

tctttattcc gcaaggaact caggaagaaa tgcgcgccga actcggcctc gatgccgctg     1860

gtatggaagc caaaatcaag gcctggctgg catgattcac acaggaaaca gctatg         1916


<210>  207
<211>  1919
<212>  DNA
<213>  artificial

<220>
<223>  (RBS3stop)dxs(RBS4stop)

<400>  207
tgattcacac aggaaacagc tatgagtttt gatattgcca aatacccgac cctggcactg       60

gtcgactcca cccaggagtt acgactgttg ccgaaagaga gtttaccgaa actctgcgac      120

gaactgcgcc gctatttact cgacagcgtg agccgttcca gcgggcactt cgcctccggg      180

ctgggcacgg tcgaactgac cgtggcgctg cactatgtct acaacacccc gtttgaccaa      240

ttgatttggg atgtggggca tcaggcttat ccgcataaaa ttttgaccgg acgccgcgac      300

aaaatcggca ccatccgtca gaaaggcggt ctgcacccgt tcccgtggcg cggcgaaagc      360

gaatatgacg tattaagcgt cgggcattca tcaacctcca tcagtgccgg aattggtatt      420

gcggttgctg ccgaaaaaga aggcaaaaat cgccgcaccg tctgtgtcat tggcgatggc      480

gcgattaccg caggcatggc gtttgaagcg atgaatcacg cgggcgatat ccgtcctgat      540

atgctggtga ttctcaacga caatgaaatg tcgatttccg aaaatgtcgg cgcgctcaac      600

aaccatctgg cacagctgct ttccggtaag ctttactctt cactgcgcga aggcgggaaa      660

aaagttttct ctggcgtgcc gccaattaaa gagctgctca aacgcaccga agaacatatt      720

aaaggcatgg tagtgcctgg cacgttgttt gaagagctgg gctttaacta catcggcccg      780

gtggacggtc acgatgtgct ggggcttatc accacgctaa agaacatgcg cgacctgaaa      840

ggcccgcagt tcctgcatat catgaccaaa aaaggtcgtg gttatgaacc ggcagaaaaa      900

gacccgatca ctttccacgc cgtgcctaaa tttgatccct ccagcggttg tttgccgaaa      960

agtagcggcg gtttgccgag ctattcaaaa atctttggcg actggttgtg cgaaacggca     1020

gcgaaagaca acaagctgat ggcgattact ccggcgatgc gtgaaggttc cggcatggtc     1080

gagttttcac gtaaattccc ggatcgctac ttcgacgtgg caattgccga gcaacacgcg     1140

gtgacctttg ctgcgggtct ggcgattggt gggtacaaac ccattgtcgc gatttactcc     1200

actttcctgc aacgcgccta tgatcaggtg ctgcatgacg tggcgattca aaagcttccg     1260

gtcctgttcg ccatcgaccg cgcgggcatt gttggtgctg acggtcaaac ccatcagggt     1320

gcttttgatc tctcttacct gcgctgcata ccggaaatgg tcattatgac cccgagcgat     1380

gaaaacgaat gtcgccagat gctctatacc ggctatcact ataacgatgg cccgtcagcg     1440

gtgcgctacc cgcgtggcaa cgcggtcggc gtggaactga cgccgctgga aaaactacca     1500

attggcaaag gcattgtgaa gcgtcgtggc gagaaactgg cgatccttaa ctttggtacg     1560

ctgatgccag aagcggcgaa agtcgccgaa tcgctgaacg ccacgctggt cgatatgcgt     1620

tttgtgaaac cgcttgatga agcgttaatt ctggaaatgg ccgccagcca tgaagcgctg     1680

gtcaccgtag aagaaaacgc cattatgggc ggcgcaggca gcggcgtgaa cgaagtgctg     1740

atggcccatc gtaaaccagt acccgtgctg aacattggcc tgccggactt ctttattccg     1800

caaggaactc aggaagaaat gcgcgccgaa ctcggcctcg atgccgctgg tatggaagcc     1860

aaaatcaagg cctggctggc ataaattaat tgttcttttt tcaggtgaag gttcccatg      1919


<210>  208
<211>  1916
<212>  DNA
<213>  artificial

<220>
<223>  (RBS4stop)dxs(N31)

<400>  208
taaattaatt gttctttttt caggtgaagg ttcccatgag ttttgatatt gccaaatacc       60

cgaccctggc actggtcgac tccacccagg agttacgact gttgccgaaa gagagtttac      120

cgaaactctg cgacgaactg cgccgctatt tactcgacag cgtgagccgt tccagcgggc      180

acttcgcctc cgggctgggc acggtcgaac tgaccgtggc gctgcactat gtctacaaca      240

ccccgtttga ccaattgatt tgggatgtgg ggcatcaggc ttatccgcat aaaattttga      300

ccggacgccg cgacaaaatc ggcaccatcc gtcagaaagg cggtctgcac ccgttcccgt      360

ggcgcggcga aagcgaatat gacgtattaa gcgtcgggca ttcatcaacc tccatcagtg      420

ccggaattgg tattgcggtt gctgccgaaa aagaaggcaa aaatcgccgc accgtctgtg      480

tcattggcga tggcgcgatt accgcaggca tggcgtttga agcgatgaat cacgcgggcg      540

atatccgtcc tgatatgctg gtgattctca acgacaatga aatgtcgatt tccgaaaatg      600

tcggcgcgct caacaaccat ctggcacagc tgctttccgg taagctttac tcttcactgc      660

gcgaaggcgg gaaaaaagtt ttctctggcg tgccgccaat taaagagctg ctcaaacgca      720

ccgaagaaca tattaaaggc atggtagtgc ctggcacgtt gtttgaagag ctgggcttta      780

actacatcgg cccggtggac ggtcacgatg tgctggggct tatcaccacg ctaaagaaca      840

tgcgcgacct gaaaggcccg cagttcctgc atatcatgac caaaaaaggt cgtggttatg      900

aaccggcaga aaaagacccg atcactttcc acgccgtgcc taaatttgat ccctccagcg      960

gttgtttgcc gaaaagtagc ggcggtttgc cgagctattc aaaaatcttt ggcgactggt     1020

tgtgcgaaac ggcagcgaaa gacaacaagc tgatggcgat tactccggcg atgcgtgaag     1080

gttccggcat ggtcgagttt tcacgtaaat tcccggatcg ctacttcgac gtggcaattg     1140

ccgagcaaca cgcggtgacc tttgctgcgg gtctggcgat tggtgggtac aaacccattg     1200

tcgcgattta ctccactttc ctgcaacgcg cctatgatca ggtgctgcat gacgtggcga     1260

ttcaaaagct tccggtcctg ttcgccatcg accgcgcggg cattgttggt gctgacggtc     1320

aaacccatca gggtgctttt gatctctctt acctgcgctg cataccggaa atggtcatta     1380

tgaccccgag cgatgaaaac gaatgtcgcc agatgctcta taccggctat cactataacg     1440

atggcccgtc agcggtgcgc tacccgcgtg gcaacgcggt cggcgtggaa ctgacgccgc     1500

tggaaaaact accaattggc aaaggcattg tgaagcgtcg tggcgagaaa ctggcgatcc     1560

ttaactttgg tacgctgatg ccagaagcgg cgaaagtcgc cgaatcgctg aacgccacgc     1620

tggtcgatat gcgttttgtg aaaccgcttg atgaagcgtt aattctggaa atggccgcca     1680

gccatgaagc gctggtcacc gtagaagaaa acgccattat gggcggcgca ggcagcggcg     1740

tgaacgaagt gctgatggcc catcgtaaac cagtacccgt gctgaacatt ggcctgccgg     1800

acttctttat tccgcaagga actcaggaag aaatgcgcgc cgaactcggc ctcgatgccg     1860

ctggtatgga agccaaaatc aaggcctggc tggcatgatg ggctgaaggg tttaag         1916


<210>  209
<211>  1842
<212>  DNA
<213>  artificial

<220>
<223>  (RBS1)valC(RBS2stop)

<400>  209
tagaaataat tttgtttaac tttaagaagg agatatacat atggccgaga tgttcaacgg       60

caactcttct aacgacggat cttcttgcat gcccgtgaag gacgccctgc gacgaaccgg      120

caaccaccac cccaacctgt ggaccgacga cttcatccag tctctgaact ctccctactc      180

tgactcttct taccacaagc accgagagat cctgatcgac gagatccgag acatgttctc      240

taacggcgag ggcgacgagt tcggcgtgct cgagaacatc tggttcgtgg acgtggtgca      300

gcgactgggc atcgaccgac acttccagga ggagatcaag accgccctgg actacatcta      360

caagttctgg aaccacgact ctatcttcgg cgacctgaac atggtggccc tgggcttccg      420

aatcctgcga ctgaaccgat acgtggcctc ttctgacgtg ttcaagaagt tcaagggcga      480

ggagggccag ttctctggct tcgagtcctc tgaccaggac gctaagctcg aaatgatgct      540

gaacctgtac aaggcctctg agctggactt ccccgacgag gacatcctga aggaggcccg      600

agccttcgcc tctatgtacc tgaagcacgt gatcaaggag tacggcgaca tccaggagtc      660

taagaacccc ctgctgatgg agatcgagta caccttcaag tacccctggc gatgccgact      720

gccccgactc gaggcctgga acttcatcca catcatgcga cagcaggact gcaacatctc      780

tctggccaac aacctctaca agatccccaa gatctacatg aagaagatcc tcgagctggc      840

catcctggac ttcaacatcc tgcagtctca gcaccagcac gagatgaagc tgatctctac      900

ctggtggaag aactcttctg ctatccagct ggacttcttc cgacaccgac acatcgagtc      960

ttacttttgg tgggcctcgc ccctgttcga gcccgagttc tctacctgcc gaatcaactg     1020

caccaagctg tctaccaaga tgttcctgct ggacgacatc tacgacacct acggcaccgt     1080

cgaggagctg aagcccttca ccaccaccct gacccgatgg gacgtgtcta ccgtggacaa     1140

ccaccccgac tacatgaaga tcgccttcaa cttctcttac gagatctaca aggagatcgc     1200

ctctgaggcc gagcgaaagc acggcccctt cgtgtacaag tacctgcagt cttgctggaa     1260

gtcttacatc gaggcctaca tgcaggaggc cgagtggatc gcctctaacc acatccccgg     1320

cttcgacgag tacctgatga acggcgtgaa gtcctctggc atgcgaatcc tgatgatcca     1380

cgccctgatc ctgatggaca cccccctgtc tgacgagatt ctcgagcagc tggacatccc     1440

ctcgtctaag tctcaggccc tgctgtctct gatcacccga ctggtggacg acgtgaagga     1500

cttcgaggac gagcaggccc acggcgagat ggcctcttct atcgagtgct acatgaagga     1560

caaccacggc tctacccgag aggacgccct gaactacctg aagatccgaa tcgagtcttg     1620

cgtgcaggag ctgaacaagg agctgctcga gccctctaac atgcacggat ctttccgaaa     1680

cctgtacctg aacgtgggaa tgcgagtgat tttcttcatg ctgaacgacg gcgacctgtt     1740

cacccactct aaccgaaagg agatccagga cgccatcacc aagttcttcg tcgagcccat     1800

catcccctaa ccgttcattt atcacaaaag gattgttcga tg                        1842


<210>  210
<211>  1823
<212>  DNA
<213>  artificial

<220>
<223>  (RBS2stop)valC(RBS3stop)

<400>  210
taaccgttca tttatcacaa aaggattgtt cgatggccga gatgttcaac ggcaactctt       60

ctaacgacgg atcttcttgc atgcccgtga aggacgccct gcgacgaacc ggcaaccacc      120

accccaacct gtggaccgac gacttcatcc agtctctgaa ctctccctac tctgactctt      180

cttaccacaa gcaccgagag atcctgatcg acgagatccg agacatgttc tctaacggcg      240

agggcgacga gttcggcgtg ctcgagaaca tctggttcgt ggacgtggtg cagcgactgg      300

gcatcgaccg acacttccag gaggagatca agaccgccct ggactacatc tacaagttct      360

ggaaccacga ctctatcttc ggcgacctga acatggtggc cctgggcttc cgaatcctgc      420

gactgaaccg atacgtggcc tcttctgacg tgttcaagaa gttcaagggc gaggagggcc      480

agttctctgg cttcgagtcc tctgaccagg acgctaagct cgaaatgatg ctgaacctgt      540

acaaggcctc tgagctggac ttccccgacg aggacatcct gaaggaggcc cgagccttcg      600

cctctatgta cctgaagcac gtgatcaagg agtacggcga catccaggag tctaagaacc      660

ccctgctgat ggagatcgag tacaccttca agtacccctg gcgatgccga ctgccccgac      720

tcgaggcctg gaacttcatc cacatcatgc gacagcagga ctgcaacatc tctctggcca      780

acaacctcta caagatcccc aagatctaca tgaagaagat cctcgagctg gccatcctgg      840

acttcaacat cctgcagtct cagcaccagc acgagatgaa gctgatctct acctggtgga      900

agaactcttc tgctatccag ctggacttct tccgacaccg acacatcgag tcttactttt      960

ggtgggcctc gcccctgttc gagcccgagt tctctacctg ccgaatcaac tgcaccaagc     1020

tgtctaccaa gatgttcctg ctggacgaca tctacgacac ctacggcacc gtcgaggagc     1080

tgaagccctt caccaccacc ctgacccgat gggacgtgtc taccgtggac aaccaccccg     1140

actacatgaa gatcgccttc aacttctctt acgagatcta caaggagatc gcctctgagg     1200

ccgagcgaaa gcacggcccc ttcgtgtaca agtacctgca gtcttgctgg aagtcttaca     1260

tcgaggccta catgcaggag gccgagtgga tcgcctctaa ccacatcccc ggcttcgacg     1320

agtacctgat gaacggcgtg aagtcctctg gcatgcgaat cctgatgatc cacgccctga     1380

tcctgatgga cacccccctg tctgacgaga ttctcgagca gctggacatc ccctcgtcta     1440

agtctcaggc cctgctgtct ctgatcaccc gactggtgga cgacgtgaag gacttcgagg     1500

acgagcaggc ccacggcgag atggcctctt ctatcgagtg ctacatgaag gacaaccacg     1560

gctctacccg agaggacgcc ctgaactacc tgaagatccg aatcgagtct tgcgtgcagg     1620

agctgaacaa ggagctgctc gagccctcta acatgcacgg atctttccga aacctgtacc     1680

tgaacgtggg aatgcgagtg attttcttca tgctgaacga cggcgacctg ttcacccact     1740

ctaaccgaaa ggagatccag gacgccatca ccaagttctt cgtcgagccc atcatcccct     1800

gattcacaca ggaaacagct atg                                             1823


<210>  211
<211>  605
<212>  DNA
<213>  artificial

<220>
<223>  (RBS3stop)idi(RBS4stop)

<400>  211
tgattcacac aggaaacagc tatgcaaacg gaacacgtca ttttattgaa tgcacaggga       60

gttcccacgg gtacgctgga aaagtatgcc gcacacacgg cagacacccg cttacatctc      120

gcgttctcca gttggctgtt taatgccaaa ggacaattat tagttacccg ccgcgcactg      180

agcaaaaaag catggcctgg cgtgtggact aactcggttt gtgggcaccc acaactggga      240

gaaagcaacg aagacgcagt gatccgccgt tgccgttatg agcttggcgt ggaaattacg      300

cctcctgaat ctatctatcc tgactttcgc taccgcgcca ccgatccgag tggcattgtg      360

gaaaatgaag tgtgtccggt atttgccgca cgcaccacta gtgcgttaca gatcaatgat      420

gatgaagtga tggattatca atggtgtgat ttagcagatg tattacacgg tattgatgcc      480

acgccgtggg cgttcagtcc gtggatggtg atgcaggcga caaatcgcga agccagaaaa      540

cgattatctg catttaccca gcttaaataa attaattgtt cttttttcag gtgaaggttc      600

ccatg                                                                  605


<210>  212
<211>  602
<212>  DNA
<213>  artificial

<220>
<223>  (RBS4stop)idi(N31)

<400>  212
taaattaatt gttctttttt caggtgaagg ttcccatgca aacggaacac gtcattttat       60

tgaatgcaca gggagttccc acgggtacgc tggaaaagta tgccgcacac acggcagaca      120

cccgcttaca tctcgcgttc tccagttggc tgtttaatgc caaaggacaa ttattagtta      180

cccgccgcgc actgagcaaa aaagcatggc ctggcgtgtg gactaactcg gtttgtgggc      240

acccacaact gggagaaagc aacgaagacg cagtgatccg ccgttgccgt tatgagcttg      300

gcgtggaaat tacgcctcct gaatctatct atcctgactt tcgctaccgc gccaccgatc      360

cgagtggcat tgtggaaaat gaagtgtgtc cggtatttgc cgcacgcacc actagtgcgt      420

tacagatcaa tgatgatgaa gtgatggatt atcaatggtg tgatttagca gatgtattac      480

acggtattga tgccacgccg tgggcgttca gtccgtggat ggtgatgcag gcgacaaatc      540

gcgaagccag aaaacgatta tctgcattta cccagcttaa atgatgggct gaagggttta      600

ag                                                                     602


<210>  213
<211>  972
<212>  DNA
<213>  artificial

<220>
<223>  (RBS1)ispA(RBS2stop)

<400>  213
tagaaataat tttgtttaac tttaagaagg agatatacat atggactttc cgcagcaact       60

cgaagcctgc gttaagcagg ccaaccaggc gctgagccgt tttatcgccc cactgccctt      120

tcagaacact cccgtggtcg aaaccatgca gtatggcgca ttattaggtg gtaagcgcct      180

gcgacctttc ctggtttatg ccaccggtca tatgttcggc gttagcacaa acacgctgga      240

cgcacccgct gccgccgttg agtgtatcca cgcttactca ttaattcatg atgatttacc      300

ggcaatggat gatgacgatc tgcgtcgcgg tttgccaacc tgccatgtga agtttggcga      360

agcaaacgcg attctcgctg gcgacgcttt acaaacgctg gcgttctcga ttttaagcga      420

tgccgatatg ccggaagtgt cggaccgcga cagaatttcg atgatttctg aactggcgag      480

cgccagtggt attgccggaa tgtgcggtgg tcaggcatta gatttagacg cggaaggcaa      540

acacgtacct ctggacgcgc ttgagcgtat tcatcgtcat aaaaccggcg cattgattcg      600

cgccgccgtt cgccttggtg cattaagcgc cggagataaa ggacgtcgtg ctctgccggt      660

actcgacaag tatgcagaga gcatcggcct tgccttccag gttcaggatg acatcctgga      720

tgtggtggga gatactgcaa cgttgggaaa acgccagggt gccgaccagc aacttggtaa      780

aagtacctac cctgcacttc tgggtcttga gcaagcccgg aagaaagccc gggatctgat      840

cgacgatgcc cgtcagtcgc tgaaacaact ggctgaacag tcactcgata cctcggcact      900

ggaagcgcta gcggactaca tcatccagcg taataaataa ccgttcattt atcacaaaag      960

gattgttcga tg                                                          972


<210>  214
<211>  953
<212>  DNA
<213>  artificial

<220>
<223>  (RBS2stop)ispA(RBS3stop)

<400>  214
taaccgttca tttatcacaa aaggattgtt cgatggactt tccgcagcaa ctcgaagcct       60

gcgttaagca ggccaaccag gcgctgagcc gttttatcgc cccactgccc tttcagaaca      120

ctcccgtggt cgaaaccatg cagtatggcg cattattagg tggtaagcgc ctgcgacctt      180

tcctggttta tgccaccggt catatgttcg gcgttagcac aaacacgctg gacgcacccg      240

ctgccgccgt tgagtgtatc cacgcttact cattaattca tgatgattta ccggcaatgg      300

atgatgacga tctgcgtcgc ggtttgccaa cctgccatgt gaagtttggc gaagcaaacg      360

cgattctcgc tggcgacgct ttacaaacgc tggcgttctc gattttaagc gatgccgata      420

tgccggaagt gtcggaccgc gacagaattt cgatgatttc tgaactggcg agcgccagtg      480

gtattgccgg aatgtgcggt ggtcaggcat tagatttaga cgcggaaggc aaacacgtac      540

ctctggacgc gcttgagcgt attcatcgtc ataaaaccgg cgcattgatt cgcgccgccg      600

ttcgccttgg tgcattaagc gccggagata aaggacgtcg tgctctgccg gtactcgaca      660

agtatgcaga gagcatcggc cttgccttcc aggttcagga tgacatcctg gatgtggtgg      720

gagatactgc aacgttggga aaacgccagg gtgccgacca gcaacttggt aaaagtacct      780

accctgcact tctgggtctt gagcaagccc ggaagaaagc ccgggatctg atcgacgatg      840

cccgtcagtc gctgaaacaa ctggctgaac agtcactcga tacctcggca ctggaagcgc      900

tagcggacta catcatccag cgtaataaat gattcacaca ggaaacagct atg             953


<210>  215
<211>  956
<212>  DNA
<213>  artificial

<220>
<223>  (RBS3stop)ispA(RBS4stop)

<400>  215
tgattcacac aggaaacagc tatggacttt ccgcagcaac tcgaagcctg cgttaagcag       60

gccaaccagg cgctgagccg ttttatcgcc ccactgccct ttcagaacac tcccgtggtc      120

gaaaccatgc agtatggcgc attattaggt ggtaagcgcc tgcgaccttt cctggtttat      180

gccaccggtc atatgttcgg cgttagcaca aacacgctgg acgcacccgc tgccgccgtt      240

gagtgtatcc acgcttactc attaattcat gatgatttac cggcaatgga tgatgacgat      300

ctgcgtcgcg gtttgccaac ctgccatgtg aagtttggcg aagcaaacgc gattctcgct      360

ggcgacgctt tacaaacgct ggcgttctcg attttaagcg atgccgatat gccggaagtg      420

tcggaccgcg acagaatttc gatgatttct gaactggcga gcgccagtgg tattgccgga      480

atgtgcggtg gtcaggcatt agatttagac gcggaaggca aacacgtacc tctggacgcg      540

cttgagcgta ttcatcgtca taaaaccggc gcattgattc gcgccgccgt tcgccttggt      600

gcattaagcg ccggagataa aggacgtcgt gctctgccgg tactcgacaa gtatgcagag      660

agcatcggcc ttgccttcca ggttcaggat gacatcctgg atgtggtggg agatactgca      720

acgttgggaa aacgccaggg tgccgaccag caacttggta aaagtaccta ccctgcactt      780

ctgggtcttg agcaagcccg gaagaaagcc cgggatctga tcgacgatgc ccgtcagtcg      840

ctgaaacaac tggctgaaca gtcactcgat acctcggcac tggaagcgct agcggactac      900

atcatccagc gtaataaata aattaattgt tcttttttca ggtgaaggtt cccatg          956


<210>  216
<211>  1823
<212>  DNA
<213>  artificial

<220>
<223>  (RBS4stop)valC(N31)

<400>  216
taaattaatt gttctttttt caggtgaagg ttcccatggc cgagatgttc aacggcaact       60

cttctaacga cggatcttct tgcatgcccg tgaaggacgc cctgcgacga accggcaacc      120

accaccccaa cctgtggacc gacgacttca tccagtctct gaactctccc tactctgact      180

cttcttacca caagcaccga gagatcctga tcgacgagat ccgagacatg ttctctaacg      240

gcgagggcga cgagttcggc gtgctcgaga acatctggtt cgtggacgtg gtgcagcgac      300

tgggcatcga ccgacacttc caggaggaga tcaagaccgc cctggactac atctacaagt      360

tctggaacca cgactctatc ttcggcgacc tgaacatggt ggccctgggc ttccgaatcc      420

tgcgactgaa ccgatacgtg gcctcttctg acgtgttcaa gaagttcaag ggcgaggagg      480

gccagttctc tggcttcgag tcctctgacc aggacgctaa gctcgaaatg atgctgaacc      540

tgtacaaggc ctctgagctg gacttccccg acgaggacat cctgaaggag gcccgagcct      600

tcgcctctat gtacctgaag cacgtgatca aggagtacgg cgacatccag gagtctaaga      660

accccctgct gatggagatc gagtacacct tcaagtaccc ctggcgatgc cgactgcccc      720

gactcgaggc ctggaacttc atccacatca tgcgacagca ggactgcaac atctctctgg      780

ccaacaacct ctacaagatc cccaagatct acatgaagaa gatcctcgag ctggccatcc      840

tggacttcaa catcctgcag tctcagcacc agcacgagat gaagctgatc tctacctggt      900

ggaagaactc ttctgctatc cagctggact tcttccgaca ccgacacatc gagtcttact      960

tttggtgggc ctcgcccctg ttcgagcccg agttctctac ctgccgaatc aactgcacca     1020

agctgtctac caagatgttc ctgctggacg acatctacga cacctacggc accgtcgagg     1080

agctgaagcc cttcaccacc accctgaccc gatgggacgt gtctaccgtg gacaaccacc     1140

ccgactacat gaagatcgcc ttcaacttct cttacgagat ctacaaggag atcgcctctg     1200

aggccgagcg aaagcacggc cccttcgtgt acaagtacct gcagtcttgc tggaagtctt     1260

acatcgaggc ctacatgcag gaggccgagt ggatcgcctc taaccacatc cccggcttcg     1320

acgagtacct gatgaacggc gtgaagtcct ctggcatgcg aatcctgatg atccacgccc     1380

tgatcctgat ggacaccccc ctgtctgacg agattctcga gcagctggac atcccctcgt     1440

ctaagtctca ggccctgctg tctctgatca cccgactggt ggacgacgtg aaggacttcg     1500

aggacgagca ggcccacggc gagatggcct cttctatcga gtgctacatg aaggacaacc     1560

acggctctac ccgagaggac gccctgaact acctgaagat ccgaatcgag tcttgcgtgc     1620

aggagctgaa caaggagctg ctcgagccct ctaacatgca cggatctttc cgaaacctgt     1680

acctgaacgt gggaatgcga gtgattttct tcatgctgaa cgacggcgac ctgttcaccc     1740

actctaaccg aaaggagatc caggacgcca tcaccaagtt cttcgtcgag cccatcatcc     1800

cctgatgggc tgaagggttt aag                                             1823


<210>  217
<211>  1128
<212>  DNA
<213>  artificial

<220>
<223>  (RBS1)aroG mutant(RBS4stop)

<400>  217
tagaaataat tttgtttaac tttaagaagg agatatacat atgaattatc agaacgacga       60

tttacgcatc aaagaaatca aagagttact tcctcctgtc gcattgctgg aaaaattccc      120

cgctactgaa aatgccgcga atacggttgc ccatgcccga aaagcgatcc ataagatcct      180

gaaaggtaat gatgatcgcc tgttggttgt gattggccca tgctcaattc atgatcctgt      240

cgcggcaaaa gagtatgcca ctcgcttgct ggcgctgcgt gaagagctga aagatgagct      300

ggaaatcgta atgcgcgtct attttgaaaa gccgcgtacc acggtgggct ggaaagggct      360

gattaacgat ccgcatatgg ataatagctt ccagatcaac gacggtctgc gtatagcccg      420

taaattgctg cttgatatta acgacagcgg tctgccagcg gcaggtgagt ttctcaatat      480

gatcacccca caatatctcg ctgacctgat gagctggggc gcaattggcg cacgtaccac      540

cgaatcgcag gtgcaccgcg aactggcatc agggctttct tgtccggtcg gcttcaaaaa      600

tggcaccgac ggtacgatta aagtggctat cgatgccatt aatgccgccg gtgcgccgca      660

ctgcttcctg tccgtaacga aatgggggca ttcggcgatt gtgaatacca gcggtaacgg      720

cgattgccat atcattctgc gcggcggtaa agagcctaac tacagcgcga agcacgttgc      780

tgaagtgaaa gaagggctga acaaagcagg cctgccagca caggtgatga tcgatttcag      840

ccatgctaac tcgtccaaac aattcaaaaa gcagatggat gtttgtgctg acgtttgcca      900

gcagattgcc ggtggcgaaa aggccattat tggcgtgatg gtggaaagcc atctggtgga      960

aggcaatcag agcctcgaga gcggggagcc gctggcctac ggtaagagca tcaccgatgc     1020

ctgcatcggc tgggaagata ccgatgctct gttacgtcaa ctggcgaatg cagtaaaagc     1080

gcgtcgcggg taaattaatt gttctttttt caggtgaagg ttcccatg                  1128


<210>  218
<211>  1197
<212>  DNA
<213>  artificial

<220>
<223>  (RBS1)tyrA mutant(RBS4stop)

<400>  218
tagaaataat tttgtttaac tttaagaagg agatatacat atggttgctg aattgaccgc       60

attacgcgat caaattgatg aagtcgataa agcgctgctg aatttattag cgaagcgtct      120

ggaactggtt gctgaagtgg gcgaggtgaa aagccgcttt ggactgccta tttatgttcc      180

ggagcgcgag gcatctattt tggcctcgcg tcgtgcagag gcggaagctc tgggtgtacc      240

gccagatctg attgaggatg ttttgcgtcg ggtgatgcgt gaatcttact ccagtgaaaa      300

cgacaaagga tttaaaacac tttgtccgtc actgcgtccg gtggttatcg tcggcggtgg      360

cggtcagatg ggacgcctgt tcgagaagat gctgaccctc tcgggttatc aggtgcggat      420

tctggagcaa catgactggg atcgagcggc tgatattgtt gccgatgccg gaatggtgat      480

tgttagtgtg ccaatccacg ttactgagca agttattggc aaattaccgc ctttaccgaa      540

agattgtatt ctggtcgatc tggcatcagt gaaaaatggg ccattacagg ccatgctggt      600

ggcgcatgat ggtccggtgc tggggctaca cccgatgttc ggtccggaca gcggtagcct      660

ggcaaagcaa gttgtggtct ggtgtgatgg acgtaaaccg gaagcatacc aatggtttct      720

ggagcaaatt caggtctggg gcgctcggct gcatcgtatt agcgccgtcg agcacgatca      780

gaatatggcg tttattcagg cactgcgcca ctttgctact tttgcttacg ggctgcacct      840

ggcagaagaa aatgttcagc ttgagcaact tctggcgctc tcttcgccga tttaccgcct      900

tgagctggcg atggtcgggc gactgtttgc tcaggatccg cagctttatg ccgacatcat      960

tatgtcgtca gagcgtaatc tggcgttaat caaacgttac tataagcgtt tcggcgaggc     1020

gattgagttg ctggagcagg gcgataagca ggcgtttatt gacagtttcc gcaaggtgga     1080

gcactggttc ggcgattacg tacagcgttt tcagagtgaa agccgcgtgt tattgcgtca     1140

ggcgaatgac aatcgccagt aaattaattg ttcttttttc aggtgaaggt tcccatg        1197


<210>  219
<211>  1175
<212>  DNA
<213>  artificial

<220>
<223>  (RBS4stop)tyrA mutant(N31)

<400>  219
taaattaatt gttctttttt caggtgaagg ttcccatggt tgctgaattg accgcattac       60

gcgatcaaat tgatgaagtc gataaagcgc tgctgaattt attagcgaag cgtctggaac      120

tggttgctga agtgggcgag gtgaaaagcc gctttggact gcctatttat gttccggagc      180

gcgaggcatc tattttggcc tcgcgtcgtg cagaggcgga agctctgggt gtaccgccag      240

atctgattga ggatgttttg cgtcgggtga tgcgtgaatc ttactccagt gaaaacgaca      300

aaggatttaa aacactttgt ccgtcactgc gtccggtggt tatcgtcggc ggtggcggtc      360

agatgggacg cctgttcgag aagatgctga ccctctcggg ttatcaggtg cggattctgg      420

agcaacatga ctgggatcga gcggctgata ttgttgccga tgccggaatg gtgattgtta      480

gtgtgccaat ccacgttact gagcaagtta ttggcaaatt accgccttta ccgaaagatt      540

gtattctggt cgatctggca tcagtgaaaa atgggccatt acaggccatg ctggtggcgc      600

atgatggtcc ggtgctgggg ctacacccga tgttcggtcc ggacagcggt agcctggcaa      660

agcaagttgt ggtctggtgt gatggacgta aaccggaagc ataccaatgg tttctggagc      720

aaattcaggt ctggggcgct cggctgcatc gtattagcgc cgtcgagcac gatcagaata      780

tggcgtttat tcaggcactg cgccactttg ctacttttgc ttacgggctg cacctggcag      840

aagaaaatgt tcagcttgag caacttctgg cgctctcttc gccgatttac cgccttgagc      900

tggcgatggt cgggcgactg tttgctcagg atccgcagct ttatgccgac atcattatgt      960

cgtcagagcg taatctggcg ttaatcaaac gttactataa gcgtttcggc gaggcgattg     1020

agttgctgga gcagggcgat aagcaggcgt ttattgacag tttccgcaag gtggagcact     1080

ggttcggcga ttacgtacag cgttttcaga gtgaaagccg cgtgttattg cgtcaggcga     1140

atgacaatcg ccagtgatgg gctgaagggt ttaag                                1175


<210>  220
<211>  1106
<212>  DNA
<213>  artificial

<220>
<223>  (RBS4stop)aroG mutant(N31)

<400>  220
taaattaatt gttctttttt caggtgaagg ttcccatgaa ttatcagaac gacgatttac       60

gcatcaaaga aatcaaagag ttacttcctc ctgtcgcatt gctggaaaaa ttccccgcta      120

ctgaaaatgc cgcgaatacg gttgcccatg cccgaaaagc gatccataag atcctgaaag      180

gtaatgatga tcgcctgttg gttgtgattg gcccatgctc aattcatgat cctgtcgcgg      240

caaaagagta tgccactcgc ttgctggcgc tgcgtgaaga gctgaaagat gagctggaaa      300

tcgtaatgcg cgtctatttt gaaaagccgc gtaccacggt gggctggaaa gggctgatta      360

acgatccgca tatggataat agcttccaga tcaacgacgg tctgcgtata gcccgtaaat      420

tgctgcttga tattaacgac agcggtctgc cagcggcagg tgagtttctc aatatgatca      480

ccccacaata tctcgctgac ctgatgagct ggggcgcaat tggcgcacgt accaccgaat      540

cgcaggtgca ccgcgaactg gcatcagggc tttcttgtcc ggtcggcttc aaaaatggca      600

ccgacggtac gattaaagtg gctatcgatg ccattaatgc cgccggtgcg ccgcactgct      660

tcctgtccgt aacgaaatgg gggcattcgg cgattgtgaa taccagcggt aacggcgatt      720

gccatatcat tctgcgcggc ggtaaagagc ctaactacag cgcgaagcac gttgctgaag      780

tgaaagaagg gctgaacaaa gcaggcctgc cagcacaggt gatgatcgat ttcagccatg      840

ctaactcgtc caaacaattc aaaaagcaga tggatgtttg tgctgacgtt tgccagcaga      900

ttgccggtgg cgaaaaggcc attattggcg tgatggtgga aagccatctg gtggaaggca      960

atcagagcct cgagagcggg gagccgctgg cctacggtaa gagcatcacc gatgcctgca     1020

tcggctggga agataccgat gctctgttac gtcaactggc gaatgcagta aaagcgcgtc     1080

gcgggtgatg ggctgaaggg tttaag                                          1106


<210>  221
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  aslA screening 4F

<400>  221
tggaacaaca ggcatggatt                                                   20


<210>  222
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  aslA screening 4R

<400>  222
acaggcgaaa tatggtgct                                                    19


<210>  223
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  nupG screening 2F

<400>  223
ggaaatatgg cgttgatgag                                                   20


<210>  224
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  nupG screening 2R

<400>  224
aggattatcc gacatcagtg                                                   20


<210>  225
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  pheA screening F

<400>  225
tcatcaaata tggctcgctt                                                   20


<210>  226
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  pheA screening R

<400>  226
tcgagcggct gatattgttg                                                   20


<210>  227
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  tyrR screening F

<400>  227
aacgctggta tgcctcaatc                                                   20


<210>  228
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  tyrR screening R

<400>  228
aggcttcctc gaatacctta                                                   20


<210>  229
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  nupG screening 4F

<400>  229
tattgtgcct atgtggcttc                                                   20


<210>  230
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  nupG screening 4R

<400>  230
cgaataaagt ggtgacgaat g                                                 21


<210>  231
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  melB screening 2F

<400>  231
gtaagcggca tggtctggaa c                                                 21


<210>  232
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  melB screening 2R

<400>  232
gcaggccgta tggactccta                                                   20


<210>  233
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  rcsB screening 3F

<400>  233
aactggcgaa tcaggcaga                                                    19


<210>  234
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  rcsB screening 3R

<400>  234
gcgattatct ctctatccgt                                                   20


<210>  235
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  ptsH/I_crr screening F

<400>  235
agaccgatct tatctctgtc                                                   20


<210>  236
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  TAGTGTAATGACCAGACAAA

<400>  236
tagtgtaatg accagacaaa                                                   20


<210>  237
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  G-gRNA-xylB F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorhioate-modified T

<400>  237
gtataatact ttgtcgattt gattttagag ctagaaatag                             40


<210>  238
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  G-gRNA-manZ F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  238
gttttaacga tatcgatacc ttttttagag ctagaaatag                             40


<210>  239
<211>  40
<212>  DNA
<213>  artificial

<220>
<223>  G-gRNA-glk F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  239
gtaatcgtta ataatttcca gattttagag ctagaaatag                             40


<210>  240
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-manZ-HF0.5


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  240
gcgggccagg tactgaccat c                                                 21


<210>  241
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  manZ-HF0.5-T R


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  241
atagtccagt tcgttatcga g                                                 21


<210>  242
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-glk-HF0.5 F


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphothioate-modified C

<400>  242
gcgcagaggg cggaaccggt g                                                 21


<210>  243
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  glk-HF0.5-T R

<400>  243
acgctaaagt caaaataatt c                                                 21


<210>  244
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-xylB-HF0.5 F


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphothioate-modified C

<400>  244
gcgtggcgat gcgcaactg                                                    19


<210>  245
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  xylB-HF0.5-T R


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphothioate-modified A

<400>  245
aagatctatc ccgatataca t                                                 21


<210>  246
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-manZ-HT0.5 F


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphothioate-modified G

<400>  246
ggactgttgt acactaccgg g                                                 21


<210>  247
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  manZ-HT0.5-T R


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphorothioate-modified A

<400>  247
aacgagaagc ttataaattt t                                                 21


<210>  248
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-glk-HT0.5 F


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphorothioate-modified A

<400>  248
gatccttcct tttatatcgg g                                                 21


<210>  249
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  glk-HT0.7-T R


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  249
agcccgcagc gtttttaatt g                                                 21


<210>  250
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-xylB-HT0.5 F


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphorothioate-modified A

<400>  250
gacgttatcc cctgcctga                                                    19


<210>  251
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  xylB-HT0.5-T R


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  251
acgaaacaaa cgcatttga                                                    19


<210>  252
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  G-pthrC3


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphothioate-modified A

<400>  252
gagcttttca ttctgactgc aa                                                22


<210>  253
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  pthrC3-T R


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  253
aggttgttac ctcgttacct t                                                 21


<210>  254
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-tal F


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  254
gacccaggtt gttgaacgtc a                                                 21


<210>  255
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  tal-T R


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  255
agccaaaatc tttaccatct gcttc                                             25


<210>  256
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  G-eGFP F


<220>
<221>  A
<222>  (2)..(2)
<223>  phosphorothioate-modified A

<400>  256
gagcaagggc gaggagctgt t                                                 21


<210>  257
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  eGFP-T R


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  257
acttgtacag ctcgtccatg c                                                 21


<210>  258
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  G-ppsA F


<220>
<221>  T
<222>  (2)..(2)
<223>  phosphorothioate-modified T

<400>  258
gtccaacaat ggctcgtca                                                    19


<210>  259
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  ppsA-T R


<220>
<221>  T
<222>  (2)..(2)
<223>  phoaphorothioate-modified T

<400>  259
attatttctt cagttcagcc agg                                               23


<210>  260
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  G-tktA F


<220>
<221>  T
<222>  (2)..(2)
<223>  phsophothioate-modified T

<400>  260
gtcctcacgt aaagagcttg                                                   20


<210>  261
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  tktA-T R


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  261
acagcagttc ttttgctttc                                                   20


<210>  262
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  G-aroE F


<220>
<221>  G
<222>  (2)..(2)
<223>  phosphorothioate-modified G

<400>  262
ggaaacctat gctgtttttg gta                                               23


<210>  263
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  aroE-T R


<220>
<221>  C
<222>  (2)..(2)
<223>  phosphorothioate-modified C

<400>  263
acgcggacaa ttcctcctgc                                                   20


<210>  264
<211>  61
<212>  DNA
<213>  artificial

<220>
<223>  CelN20-Noligo F

<400>  264
gagggtaata cacgcgaaga caactttaag cacttattgg gtaacgacaa cgttaagcgc       60

t                                                                       61


<210>  265
<211>  61
<212>  DNA
<213>  artificial

<220>
<223>  CelN20-Noligo R

<400>  265
gcgcttaacg ttgtcgttac ccaataagtg cttaaagttg tcttcgcgtg tattaccctc       60

c                                                                       61


<210>  266
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  AS-Noligo F

<400>  266
agtcgacctg caggtatgtt aatatggact act                                    33


<210>  267
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  AS-Noligo R

<400>  267
gtagtccata ttaacatacc tgcaggtcga ctc                                    33


<210>  268
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  N21 sticky end

<400>  268
cctcgactca cactt                                                        15


<210>  269
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N21 complete sequence

<400>  269
tccctcgact cacacttgg                                                    19


<210>  270
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  N22 sticky end

<400>  270
acacaacata gccac                                                        15


<210>  271
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N22 complete sequence

<400>  271
tcacacaaca tagccacgg                                                    19


<210>  272
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  N23 sticky end

<400>  272
caaacagaaa ggccat                                                       16


<210>  273
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N23 complete sequence

<400>  273
tcaaacagaa aggccatgg                                                    19


<210>  274
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  N24 sticky end

<400>  274
ctcatggatt ctacg                                                        15


<210>  275
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N24 complete sequence

<400>  275
tcctcatgga ttctacggg                                                    19


<210>  276
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  3UTR1 sticky end

<400>  276
tgggctgaag ggttt                                                        15


<210>  277
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  3UTR1 complete sequence

<400>  277
gatgggctga agggtttaa                                                    19


<210>  278
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  N32 sticky end

<400>  278
cccatcacag cttac                                                        15


<210>  279
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N32 complete sequence

<400>  279
gtcccatcac agcttacaa                                                    19


<210>  280
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  N36 sticky end

<400>  280
taagggcgaa tgtac                                                        15


<210>  281
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N36 complete sequence

<400>  281
actaagggcg aatgtacag                                                    19


<210>  282
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  N37 sticky end

<400>  282
ctttgggtca tgtgc                                                        15


<210>  283
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N37  complete sequence

<400>  283
aactttgggt catgtgctg                                                    19


<210>  284
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  N38 sticky end

<400>  284
actaaaccac agcct                                                        15


<210>  285
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N38 complete sequence

<400>  285
tcactaaacc acagcctac                                                    19


<210>  286
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  N39 sticky end

<400>  286
gtgggacatc tgttt                                                        15


<210>  287
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N39 complete sequence

<400>  287
acgtgggaca tctgtttcg                                                    19


<210>  288
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  N40 sticky end

<400>  288
gcggaacccc tattt                                                        15


<210>  289
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N40 complete sequence

<400>  289
gtgcgcggaa cccctattt                                                    19


<210>  290
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119 sticky end

<400>  290
tagctcagtc ctagg                                                        15


<210>  291
<211>  10
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119 right

<400>  291
tataatacta                                                              10


<210>  292
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119 complete sequence

<400>  292
tgacagctag ctcagtccta ggtataatac ta                                     32


<210>  293
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  SRBS2 sticky end

<400>  293
ttcatttatc acaaaagga                                                    19


<210>  294
<211>  33
<212>  DNA
<213>  artificial

<220>
<223>  SRBS2 complete sequence

<400>  294
aaccgttcat ttatcacaaa aggattgttc gat                                    33


<210>  295
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  SRBS3 sticky end

<400>  295
acacaggaaa cagct                                                        15


<210>  296
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  SRBS3 complete sequence

<400>  296
gattcacaca ggaaacagct at                                                22


<210>  297
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  SRBS4 left

<400>  297
aaattaattg ttctttttt                                                    19


<210>  298
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  SRBS4 sticky end

<400>  298
caggtgaagg ttccc                                                        15


<210>  299
<211>  36
<212>  DNA
<213>  artificial

<220>
<223>  SRBS4 complete sequence

<400>  299
aaattaattg ttcttttttc aggtgaaggt tcccat                                 36


<210>  300
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  SRBS5 left

<400>  300
gactccatac ccgtt                                                        15


<210>  301
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  SRBS5 sticky end

<400>  301
ttttgggcta acagg                                                        15


<210>  302
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  SRBS5 right

<400>  302
aggaggaatt aaccat                                                       16


<210>  303
<211>  46
<212>  DNA
<213>  artificial

<220>
<223>  SRBS5 complete sequence

<400>  303
gactccatac ccgttttttg ggctaacagg aggaggaatt aaccat                      46


<210>  304
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  T2A left

<400>  304
caggaagcgg agagggcaga ggaagtctgc                                        30


<210>  305
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  T2A sticky end

<400>  305
taacatgcgg tgacg                                                        15


<210>  306
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  T2A right

<400>  306
tcgaggagaa tcctggtcct gg                                                22


<210>  307
<211>  67
<212>  DNA
<213>  artificial

<220>
<223>  T2A complete sequence

<400>  307
caggaagcgg agagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg       60

gtcctgg                                                                 67


<210>  308
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  F2A left

<400>  308
caggaagcgg agtgaaacag actttgaatt                                        30


<210>  309
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  F2A sticky end

<400>  309
tcgaccttct caagt                                                        15


<210>  310
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  F2A right

<400>  310
tggcgggaga cgtggagtcc aaccctggtc c                                      31


<210>  311
<211>  76
<212>  DNA
<213>  artificial

<220>
<223>  F2A complete sequence

<400>  311
caggaagcgg agtgaaacag actttgaatt tcgaccttct caagttggcg ggagacgtgg       60

agtccaaccc tggtcc                                                       76


<210>  312
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  LK3 sticky end

<400>  312
ggctcgtctt cttca                                                        15


<210>  313
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  LK3 complete sequence

<400>  313
caggctcgtc ttcttcagg                                                    19


<210>  314
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  LK5 sticky end

<400>  314
tcatcgtcag gcacc                                                        15


<210>  315
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  LK5 complete sequence

<400>  315
cctcatcgtc aggcacctc                                                    19


<210>  316
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  LK6 sticky end

<400>  316
gacaacctgt atttcc                                                       16


<210>  317
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  LK6 complete sequence

<400>  317
ataaggacaa cctgtatttc caggg                                             25


<210>  318
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  LK7 sticky end

<400>  318
agcaggagca cacat                                                        15


<210>  319
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  LK7 complete sequence

<400>  319
cagcagcagg agcacacat                                                    19


<210>  320
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  Shistag sticky end

<400>  320
catcatcatc accatc                                                       16


<210>  321
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  Shistag complete sequence

<400>  321
ctcatcatca tcaccatcac tga                                               23


<210>  322
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  5UTR sticky end

<400>  322
gcaatctaat ctaagtt                                                      17


<210>  323
<211>  12
<212>  DNA
<213>  artificial

<220>
<223>  5UTR right

<400>  323
ttctagaaaa at                                                           12


<210>  324
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  5UTR complete sequence

<400>  324
agcaatctaa tctaagtttt ctagaaaaat                                        30


<210>  325
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  5UTR2 sticky end

<400>  325
tcgacggatt ctagta                                                       16


<210>  326
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  5UTR2 complete sequence

<400>  326
tcgacggatt ctagtaaaat cat                                               23


<210>  327
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  5UTR3 sticky end

<400>  327
caactatcaa aacacaa                                                      17


<210>  328
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  5UTR3 complete sequence

<400>  328
tgaacaacta tcaaaacaca atgat                                             25


<210>  329
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  5UTR4 sticky end

<400>  329
gcaatctaat ctaagtt                                                      17


<210>  330
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  5UTR4 right

<400>  330
ttaattacaa atctagaat                                                    19


<210>  331
<211>  37
<212>  DNA
<213>  artificial

<220>
<223>  5UTR4 complete sequence

<400>  331
agcaatctaa tctaagtttt aattacaaat ctagaat                                37


<210>  332
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  5UTR5 sticky end

<400>  332
gcaatctaat ctaagtt                                                      17


<210>  333
<211>  13
<212>  DNA
<213>  artificial

<220>
<223>  5UTR5 right

<400>  333
ttaattacaa aat                                                          13


<210>  334
<211>  31
<212>  DNA
<213>  artificial

<220>
<223>  5UTR5 complete sequence

<400>  334
agcaatctaa tctaagtttt aattacaaaa t                                      31


<210>  335
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  5UTR6 sticky end

<400>  335
gcaatctaat ctaagtt                                                      17


<210>  336
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  5UTR6 complete sequence

<400>  336
agcaatctaa tctaagttta ccccat                                            26


<210>  337
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  5UTR7 sticky end

<400>  337
gcaatctaat ctaagtt                                                      17


<210>  338
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  5UTR7 complete sequence

<400>  338
agcaatctaa tctaagttta aaat                                              24


<210>  339
<211>  17
<212>  DNA
<213>  3UTR2 sticky end

<400>  339
catcatcatc accatca                                                      17


<210>  340
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  3UTR2 complete sequence

<400>  340
aacatcatca tcaccatcac taaaa                                             25


<210>  341
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  3UTR3 sticky end

<400>  341
tgatttctga agaagatt                                                     18


<210>  342
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  3UTR3 complete sequence

<400>  342
ctcaaaaatt gatttctgaa gaagatttgt aatga                                  35


<210>  343
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  3UTR4 sticky end

<400>  343
catcatcacc atcac                                                        15


<210>  344
<211>  15
<212>  DNA
<213>  artificial

<220>
<223>  3UTR4 right

<400>  344
catcatcatt aatga                                                        15


<210>  345
<211>  38
<212>  DNA
<213>  artificial

<220>
<223>  3UTR4 complete sequence

<400>  345
ctctcgagca tcatcaccat caccatcatc attaatga                               38


<210>  346
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  3UTR5 sticky end

<400>  346
gcaatcgcat cacatt                                                       16


<210>  347
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  3UTR5 complete sequence

<400>  347
gacgggcaat cgcatcacat tcaaga                                            26


<210>  348
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  3UTR6 sticky end

<400>  348
ttatgtcacg cttaca                                                       16


<210>  349
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  3UTR6 complete sequence

<400>  349
attagttatg tcacgcttac attcac                                            26


<210>  350
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  3UTR7 sticky end

<400>  350
catcatcatc atcacc                                                       16


<210>  351
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  3UTR7 complete sequence

<400>  351
catcatcatc atcacc                                                       16


<210>  352
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  ICeuI sticky end

<400>  352
ataacggtcc taaggta                                                      17


<210>  353
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  ICeuI complete sequence

<400>  353
aactataacg gtcctaaggt agcgaa                                            26


<210>  354
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  SEC17_L sticky end

<400>  354
atgtagtagg gaaatata                                                     18


<210>  355
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  SEC17_L  complete sequence

<400>  355
atgtagtagg gaaatatatc aaa                                               23


<210>  356
<211>  16
<212>  DNA
<213>  artificial

<220>
<223>  SEC17_R sticky end

<400>  356
gatatttccc gttgtg                                                       16


<210>  357
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  SEC17_R complete sequence

<400>  357
tgatatttcc cgttgtgtta a                                                 21


<210>  358
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  EFB1_L sticky end

<400>  358
atgttccgat ttagttta                                                     18


<210>  359
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  EFB1_L complete sequence

<400>  359
atgttccgat ttagtttact ttata                                             25


<210>  360
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  EFB1_R sticky end

<400>  360
gttttgtttc tcctttta                                                     18


<210>  361
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  EFB1_R complete sequence

<400>  361
gttttgtttc tccttttaaa ata                                               23


<210>  362
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N21 RG-f

<400>  362
cctcgactca cacttggg                                                     18


<210>  363
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N21-RGf


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  363
ccaagtgtga gtcgagg                                                      17


<210>  364
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N22 RG-f

<400>  364
acacaacata gccacggg                                                     18


<210>  365
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N22 Rg-r


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  365
ccgtggctat gttgtgt                                                      17


<210>  366
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N23 RG-f

<400>  366
caaacagaaa ggccatggg                                                    19


<210>  367
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N23 RG-r


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  367
ccatggcctt tctgtttg                                                     18


<210>  368
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N24 RG-f

<400>  368
ctcatggatt ctacgggg                                                     18


<210>  369
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N24 RG-r


<220>
<221>  C
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  369
cccgtagaat ccatgag                                                      17


<210>  370
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119 RG-f

<400>  370
tagctcagtc ctaggtataa tactag                                            26


<210>  371
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119 RG-r


<220>
<221>  T
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  371
tagtattata cctaggactg agcta                                             25


<210>  372
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N21 LG-f


<220>
<221>  T
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  372
tccctcgact cacactt                                                      17


<210>  373
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N21 LG-r

<400>  373
aagtgtgagt cgagggaa                                                     18


<210>  374
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N22 LG-f


<220>
<221>  T
<222>  (1)..(1)
<223>  5'-phosphoryltated

<400>  374
tcacacaaca tagccac                                                      17


<210>  375
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N22 LG-r

<400>  375
gtggctatgt tgtgtgaa                                                     18


<210>  376
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N23 LG-f


<220>
<221>  T
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  376
tcaaacagaa aggccat                                                      17


<210>  377
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N23 LG-r

<400>  377
atggcctttc tgtttgaa                                                     18


<210>  378
<211>  17
<212>  DNA
<213>  artificial

<220>
<223>  N24 LG-f


<220>
<221>  T
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  378
tcctcatgga ttctacg                                                      17


<210>  379
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N24 LG-r

<400>  379
cgtagaatcc atgaggaa                                                     18


<210>  380
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119 LG-f


<220>
<221>  T
<222>  (1)..(1)
<223>  5'-phosphorylated

<400>  380
tgacagctag ctcagtccta gg                                                22


<210>  381
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119 LG-r

<400>  381
cctaggactg agctagctgt caa                                               23


<210>  382
<211>  69
<212>  DNA
<213>  artificial

<220>
<223>  RBS1 RG-Boligo

<400>  382
atatgtatat ctccttctta aagttaaaca atatgttttg tttaacttta agaaggagat       60

atacatatg                                                               69


<210>  383
<211>  65
<212>  DNA
<213>  artificial

<220>
<223>  RBS1 LA-Boligo

<400>  383
agaaataatt ttgtttaact ttaagaaggt ctactccttc ttaaagttaa acaaaattat       60

ttcta                                                                   65


<210>  384
<211>  63
<212>  DNA
<213>  artificial

<220>
<223>  SRBS2 RG-Boligo

<400>  384
atcgaacaat ccttttgtga taaatgaatc ggttttcatt tatcacaaaa ggattgttcg       60

atg                                                                     63


<210>  385
<211>  55
<212>  DNA
<213>  artificial

<220>
<223>  SRBS2 LA-Boligo

<400>  385
aaccgttcat ttatcacaaa aggatatcac tccttttgtg ataaatgaac ggtta            55


<210>  386
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  SRBS3 RG-Boligo

<400>  386
atagctgttt cctgtgtgcc tggacacagg aaacagctat g                           41


<210>  387
<211>  47
<212>  DNA
<213>  artificial

<220>
<223>  SRBS3 LA-Boligo

<400>  387
gattcacaca ggaaacagct tcttcgagct gtttcctgtg tgaatca                     47


<210>  388
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  SRBS4 RG-Boligo

<400>  388
atgggaacct tcacctgaag ttacaggtga aggttcccat g                           41


<210>  389
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  SRBS4 LA-Boligo

<400>  389
atgggaacct tcacctgaag ttacaggtga aggttcccat g                           41


<210>  390
<211>  57
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119 RG-Boligo

<400>  390
tagtattata cctaggactg agctaagagg gtagctcagt cctaggtata atactag          57


<210>  391
<211>  51
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119 LA-Boligo

<400>  391
tgacagctag ctcagtccta gggcacagcc taggactgag ctagctgtca a                51


<210>  392
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N21 RG-Boligo

<400>  392
ccaagtgtga gtcgaggaag ggccctcgac tcacacttgg g                           41


<210>  393
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N21 LA-Boligo

<400>  393
tccctcgact cacacttgcg agaaagtgtg agtcgaggga a                           41


<210>  394
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N22 RG-Boligo

<400>  394
ccgtggctat gttgtgtcgt attacacaac atagccacgg g                           41


<210>  395
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N22 LA-Boligo

<400>  395
tcacacaaca tagccacttg ctggtggcta tgttgtgtga a                           41


<210>  396
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  N23 RG-Boligo

<400>  396
ccatggcctt tctgtttgac catacaaaca gaaaggccat ggg                         43


<210>  397
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N23 LA-Boligo

<400>  397
tcaaacagaa aggccatcat tcaatggcct ttctgtttga a                           41


<210>  398
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N24 RG-Boligo

<400>  398
cccgtagaat ccatgagaac ccgctcatgg attctacggg g                           41


<210>  399
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N24 LA-Boligo

<400>  399
tcctcatgga ttctacgact atgcgtagaa tccatgagga a                           41


<210>  400
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  3UTR1 RG-Boligo

<400>  400
ttaaaccctt cagcccacac acatgggctg aagggtttaa g                           41


<210>  401
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  3UTR1 LA-Boligo

<400>  401
gatgggctga agggtttcac acaaaaccct tcagcccatc a                           41


<210>  402
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N32 RG-Boligo

<400>  402
ttgtaagctg tgatggggcc tggcccatca cagcttacaa g                           41


<210>  403
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N32 LA-Boligo

<400>  403
gtcccatcac agcttacgct tcggtaagct gtgatgggac a                           41


<210>  404
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  LK3 RG-Boligo

<400>  404
cctgaagaag acgagcccac acaggctcgt cttcttcagg g                           41


<210>  405
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  LK3 LA-Boligo

<400>  405
caggctcgtc ttcttcacac acatgaagaa gacgagcctg a                           41


<210>  406
<211>  65
<212>  DNA
<213>  artificial

<220>
<223>  5UTR RG-Boligo

<400>  406
atttttctag aaaacttaga ttagattgca agttagcaat ctaatctaag ttttctagaa       60

aaatg                                                                   65


<210>  407
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  5UTR LA-Boligo

<400>  407
agcaatctaa tctaagttgc acataactta gattagattg cta                         43


<210>  408
<211>  53
<212>  DNA
<213>  artificial

<220>
<223>  3UTR2 RG-Boligo

<400>  408
ttttagtgat ggtgatgatg atgggcaatc atcatcatca ccatcactaa aag              53


<210>  409
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  3UTR2 LA-Boligo

<400>  409
aacatcatca tcaccatcaa tgtactgatg gtgatgatga tgtta                       45


<210>  410
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N36 RA-Boligo

<400>  410
ctgtacattc gcccttaagc ttgtaagggc gaatgtacag a                           41


<210>  411
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N36 LG-Boligo

<400>  411
actaagggcg aatgtacagc ttggtacatt cgcccttagt g                           41


<210>  412
<211>  51
<212>  DNA
<213>  artificial

<220>
<223>  ICeuI RA-Boligo

<400>  412
ttcgctacct taggaccgtt atagcttgat aacggtccta aggtagcgaa a                51


<210>  413
<211>  49
<212>  DNA
<213>  artificial

<220>
<223>  ICeuI LG-Boligo

<400>  413
aactataacg gtcctaaggt aagcttgtac cttaggaccg ttatagttg                   49


<210>  414
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N22 RA-Boligo

<400>  414
ccgtggctat gttgtgtagc ttgacacaac atagccacgg a                           41


<210>  415
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N22 LG-Boligo

<400>  415
tcacacaaca tagccacagc ttggtggcta tgttgtgtga g                           41


<210>  416
<211>  53
<212>  DNA
<213>  artificial

<220>
<223>  3UTR2 RA-Boligo

<400>  416
ttttagtgat ggtgatgatg atgggcaatc atcatcatca ccatcactaa aaa              53


<210>  417
<211>  45
<212>  DNA
<213>  artificial

<220>
<223>  3UTR2 LG-Boligo

<400>  417
aacatcatca tcaccatcaa tgtactgatg gtgatgatga tgttg                       45


<210>  418
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N21 RA-Boligo

<400>  418
ccaagtgtga gtcgaggaag ggccctcgac tcacacttgg a                           41


<210>  419
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N21 LG-Boligo

<400>  419
tccctcgact cacacttgcg agaaagtgtg agtcgaggga g                           41


<210>  420
<211>  43
<212>  DNA
<213>  artificial

<220>
<223>  N23 RA-Boligo

<400>  420
ccatggcctt tctgtttgac catacaaaca gaaaggccat gga                         43


<210>  421
<211>  41
<212>  DNA
<213>  artificial

<220>
<223>  N23 LG-Boligo

<400>  421
tcaaacagaa aggccatcat tcaatggcct ttctgtttga g                           41


<210>  422
<211>  32
<212>  DNA
<213>  artificial

<220>
<223>  RBS1-G-Assemble


<220>
<221>  T
<222>  (9)..(9)
<223>  phosphorothioate-modified T

<220>
<221>  A
<222>  (20)..(20)
<223>  phosphorothioate-modified A

<400>  422
ttgtttaact ttaagaagga gatatacata tg                                     32


<210>  423
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  RBS1-T-Assemble


<220>
<221>  A
<222>  (9)..(9)
<223>  phosphorothioate-modified A

<220>
<221>  A
<222>  (20)..(20)
<223>  phosphorothioate-modified A

<400>  423
ccttcttaaa gttaaacaaa attatttcta                                        30


<210>  424
<211>  29
<212>  DNA
<213>  artificial

<220>
<223>  SRBS2-G-Assemble


<220>
<221>  C
<222>  (9)..(9)
<223>  phosporothioate-modified C

<220>
<221>  T
<222>  (20)..(20)
<223>  phosporothioate-modified T

<400>  424
ttcatttatc acaaaaggat tgttcgatg                                         29


<210>  425
<211>  25
<212>  DNA
<213>  artificial

<220>
<223>  SRBS2-T-Assemble


<220>
<221>  G
<222>  (9)..(9)
<223>  phosphorothioate-modified G

<220>
<221>  C
<222>  (20)..(20)
<223>  phosphorothioate-modified G

<400>  425
tccttttgtg ataaatgaac ggtta                                             25


<210>  426
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  SRBS3-G-Assemble


<220>
<221>  A
<222>  (9)..(9)
<223>  phosphorothiioate-modified A

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphorothiioate-modified A

<400>  426
acacaggaaa cagctatg                                                     18


<210>  427
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  SRBS3-T-Assemble


<220>
<221>  C
<222>  (9)..(9)
<223>  phosphorothioate-modified C

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  427
agctgtttcc tgtgtgaatc a                                                 21


<210>  428
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  SRBS4-G-Assemble


<220>
<221>  G
<222>  (9)..(9)
<223>  phosphorothioate-modified G

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphorothioate-modified A

<400>  428
caggtgaagg ttcccatg                                                     18


<210>  429
<211>  35
<212>  DNA
<213>  artificial

<220>
<223>  SRBS4-T-Assemble


<220>
<221>  T
<222>  (9)..(9)
<223>  phosphorothioate-modified T

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphorothioate-modified A

<400>  429
gggaaccttc acctgaaaaa agaacaatta attta                                  35


<210>  430
<211>  26
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119-G-Assemble


<220>
<221>  G
<222>  (8)..(8)
<223>  phosphorothioate-modfied G

<220>
<221>  T
<222>  (16)..(16)
<223>  phosphorothioate-modfied G

<400>  430
tagctcagtc ctaggtataa tactag                                            26


<210>  431
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  pJ23119-T-Assemble


<220>
<221>  C
<222>  (8)..(8)
<223>  phosphorothioate-modified C

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified C

<400>  431
cctaggactg agctagctgt caa                                               23


<210>  432
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N21-G-Assemble


<220>
<221>  T
<222>  (8)..(8)
<223>  phosphorothioate-modified T

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  432
cctcgactca cacttggg                                                     18


<210>  433
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N21-T-Assemble


<220>
<221>  A
<222>  (8)..(8)
<223>  phosphorothioate-modified A

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  433
aagtgtgagt cgagggaa                                                     18


<210>  434
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N22-G-Assemble


<220>
<221>  A
<222>  (8)..(8)
<223>  phosphorothioate-modified A

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  434
acacaacata gccacggg                                                     18


<210>  435
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N22-T-Assemble


<220>
<221>  T
<222>  (8)..(8)
<223>  phosphorothioate-modified T

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  435
gtggctatgt tgtgtgaa                                                     18


<210>  436
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N23-G-Assemble


<220>
<221>  A
<222>  (9)..(9)
<223>  phosphorothioate-modified A

<220>
<221>  G
<222>  (17)..(17)
<223>  phosphorothioate-modified G

<400>  436
caaacagaaa ggccatggg                                                    19


<210>  437
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N23-T-Assemble


<220>
<221>  T
<222>  (9)..(9)
<223>  phosphorothioate-modified T

<220>
<221>  A
<222>  (17)..(17)
<223>  phosphorothioate-modified A

<400>  437
atggcctttc tgtttgaa                                                     18


<210>  438
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N24-G-Assemble


<220>
<221>  A
<222>  (8)..(8)
<223>  phosphorothioate-modified A

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  438
ctcatggatt ctacggggg                                                    19


<210>  439
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N24-T-Assemble


<220>
<221>  T
<222>  (8)..(8)
<223>  phosphorothioate-modified T

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  439
cgtagaatcc atgaggaa                                                     18


<210>  440
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  3UTR1-G-Assemble


<220>
<221>  A
<222>  (8)..(8)
<223>  phosphorothioate-modified A

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphorothioate-modified A

<400>  440
tgggctgaag ggtttaag                                                     18


<210>  441
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  3UTR1-T-Assemble


<220>
<221>  T
<222>  (8)..(8)
<223>  phosphorothioate-modified T

<220>
<221>  T
<222>  (16)..(16)
<223>  phosphorothioate-modified T

<400>  441
aaacccttca gcccatca                                                     18


<210>  442
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N32-G-Assemble


<220>
<221>  A
<222>  (9)..(9)
<223>  phosphorothioate-modified A

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphorothioate-modified A

<400>  442
cccatcacag cttacaag                                                     18


<210>  443
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N32-T-Assemble


<220>
<221>  T
<222>  (9)..(9)
<223>  phosphorothioate-modified T

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphorothioate-modified A

<400>  443
gtaagctgtg atgggaca                                                     18


<210>  444
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  LK3-G-Assemble


<220>
<221>  C
<222>  (8)..(8)
<223>  phosphorothioate-modified C

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified 16

<400>  444
ggctcgtctt cttcaggg                                                     18


<210>  445
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  LK3-T-Assemble


<220>
<221>  G
<222>  (8)..(8)
<223>  phosphorothioate-modified G

<220>
<221>  T
<222>  (16)..(16)
<223>  phosphorothioate-modified T

<400>  445
tgaagaagac gagcctga                                                     18


<210>  446
<211>  30
<212>  DNA
<213>  artificial

<220>
<223>  fiveUTR-G-Assemble


<220>
<221>  T
<222>  (10)..(10)
<223>  phosphorothioate-modified T

<220>
<221>  T
<222>  (17)..(17)
<223>  phosphorothioate-modified T

<400>  446
gcaatctaat ctaagttttc tagaaaaatg                                        30


<210>  447
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  fiveUTR-T-Assemble


<220>
<221>  T
<222>  (10)..(10)
<223>  phosphorothioate-modified T

<220>
<221>  T
<222>  (17)..(17)
<223>  phosphorothioate-modified T

<400>  447
aacttagatt agattgcta                                                    19


<210>  448
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  3UTR2-G-Assemble


<220>
<221>  C
<222>  (10)..(10)
<223>  phosphorothioate-modified C

<220>
<221>  C
<222>  (18)..(18)
<223>  phosphorothioate-modified C

<400>  448
catcatcatc accatcacta aaag                                              24


<210>  449
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  3UTR2-T-Assemble


<220>
<221>  T
<222>  (10)..(10)
<223>  phosphorothioate-modified T

<220>
<221>  T
<222>  (18)..(18)
<223>  phosphorothioate-modified T

<400>  449
tgatggtgat gatgatgtta                                                   20


<210>  450
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N36-A-Assemble


<220>
<221>  A
<222>  (9)..(9)
<223>  phosphorothioate-modified A

<220>
<221>  A
<222>  (16)..(16)
<223>  phosphorothioate-modified A

<400>  450
taagggcgaa tgtacaga                                                     18


<210>  451
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N36-C-Assemble


<220>
<221>  G
<222>  (9)..(9)
<223>  phosphorothioate-modified G

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  451
gtacattcgc ccttagtg                                                     18


<210>  452
<211>  23
<212>  DNA
<213>  artificial

<220>
<223>  ICeuI-A-Assemble


<220>
<221>  C
<222>  (10)..(10)
<223>  phosphorothioate-modified C

<220>
<221>  G
<222>  (18)..(18)
<223>  phosphorothioate-modified G

<400>  452
ataacggtcc taaggtagcg aaa                                               23


<210>  453
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  ICeuI-C-Assemble


<220>
<221>  A
<222>  (10)..(10)
<223>  phosphorothioate-modified A

<220>
<221>  A
<222>  (18)..(18)
<223>  phosphorothioate-modified A

<400>  453
taccttagga ccgttatagt tg                                                22


<210>  454
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N22-A-Assemble


<220>
<221>  T
<222>  (9)..(9)
<223>  phosphorothioate-modified T

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  454
acacaacata gccacgga                                                     18


<210>  455
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N22-C-Assemble


<220>
<221>  G
<222>  (9)..(9)
<223>  phosphorothioate-modified G

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  455
gtggctatgt tgtgtgag                                                     18


<210>  456
<211>  24
<212>  DNA
<213>  artificial

<220>
<223>  3UTR2-A-Assemble


<220>
<221>  C
<222>  (10)..(10)
<223>  phosphorothioate-modified C

<220>
<221>  C
<222>  (18)..(18)
<223>  phosphorothioate-modified C

<400>  456
catcatcatc accatcacta aaaa                                              24


<210>  457
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  3UTR2-C-Assemble


<220>
<221>  T
<222>  (10)..(10)
<223>  phosphorothioate-modified T

<220>
<221>  T
<222>  (18)..(18)
<223>  phosphorothioate-modified T

<400>  457
tgatggtgat gatgatgttg                                                   20


<210>  458
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N21-A-Assemble


<220>
<221>  T
<222>  (8)..(8)
<223>  phosphorothioate-modified T

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  458
cctcgactca cacttgga                                                     18


<210>  459
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N21-C-Assemble


<220>
<221>  A
<222>  (8)..(8)
<223>  phosphorothioate-modified A

<220>
<221>  G
<222>  (16)..(16)
<223>  phosphorothioate-modified G

<400>  459
aagtgtgagt cgagggag                                                     18


<210>  460
<211>  19
<212>  DNA
<213>  artificial

<220>
<223>  N23-A-Assemble


<220>
<221>  A
<222>  (9)..(9)
<223>  phosphorothioate-modfiied A

<220>
<221>  G
<222>  (17)..(17)
<223>  phosphorothioate-modfiied G

<400>  460
caaacagaaa ggccatgga                                                    19


<210>  461
<211>  18
<212>  DNA
<213>  artificial

<220>
<223>  N23-C-Assemble


<220>
<221>  T
<222>  (9)..(9)
<223>  phosphorothioate-modified T

<220>
<221>  A
<222>  (17)..(17)
<223>  phosphorothioate-modified A

<400>  461
atggcctttc tgtttgag                                                     18


<210>  462
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  xylB screening F

<400>  462
tcctgaaaca gtttggtctg ga                                                22


<210>  463
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  xylB screening R

<400>  463
catggatagc tctcgttggt                                                   20


<210>  464
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  RO1-f (p5_library_f)

<400>  464
tagaccctct gtaaattccg                                                   20


<210>  465
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  ppsA-r (ppsA_library_r)

<400>  465
aagctgagta acatcgtcaa ta                                                22


<210>  466
<211>  21
<212>  DNA
<213>  artificial

<220>
<223>  tal-f

<400>  466
tgaactggca ggtatttgtc c                                                 21


<210>  467
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  AR1-r (AmpR_library_r)

<400>  467
taagggcgac acggaaatgt                                                   20


<210>  468
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  RO2-f (p15_library_f)

<400>  468
caagagatta cgcgcagacc                                                   20


<210>  469
<211>  22
<212>  DNA
<213>  artificial

<220>
<223>  ppsA-r

<400>  469
aagctgagta acatcgtcaa ta                                                22


<210>  470
<211>  20
<212>  DNA
<213>  artificial

<220>
<223>  AR2-r (SpecR_library_r)

<400>  470
agccgtacaa atgtacggcc                                                   20


