                         SEQUENCE LISTING

<110>  Base4 Innovation Limited
 
<120>  SINGLE NUCLEOTIDE ANALYTICAL METHOD AND ASSOCIATED PROBES

<130>  P113182EP

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  61
<212>  DNA
<213>  Artificial sequence

<220>
<223>  single-stranded first oligonucleotide 1a


<220>
<221>  T labelled with Atto 700 dye
<222>  (31)..(31)
<223>  F represents a deoxythymidine nucleotide (T) labelled with Atto 
       700 dye

<220>
<221>  T labelled with Atto 700 dye
<222>  (32)..(32)
<223>  F represents a deoxythymidine nucleotide (T) labelled with Atto 
       700 dye

<220>
<221>  T labelled with a BHQ-2 quencher
<222>  (33)..(33)
<223>  Q represents a deoxythymidine nucleotide labelled with a BHQ-2 
       quencher

<220>
<221>  inverted 3' dT nucleotide
<222>  (61)..(61)
<223>  X represents an inverted 3' dT nucleotide

<400>  1
tttcgggtga ggtcatggtc gacaggtggg tttagatgat gatcagatgt tgcccttagc       60

t                                                                       61


<210>  2
<211>  61
<212>  DNA
<213>  Artificial sequence

<220>
<223>  single-stranded oligonucleotide 1b


<220>
<221>  (T) labelled with Atto 700 dye
<222>  (31)..(31)
<223>  F represents a deoxythymidine nucleotide (T) labelled with Atto 
       700 dye

<220>
<221>  (T) labelled with Atto 700 dye
<222>  (32)..(32)
<223>  F represents a deoxythymidine nucleotide (T) labelled with Atto 
       700 dye

<220>
<221>  (T) labelled with a BHQ-2 quencher
<222>  (33)..(33)
<223>  Q represents a deoxythymidine nucleotide labelled with a BHQ-2 
       quencher

<220>
<221>  inverted 3' dT nucleotide
<222>  (61)..(61)
<223>  X represents an inverted 3' dT nucleotide

<400>  2
tttcgagtga ggtcatggtc gacaggtggg tttagatgat gatcagatgt tgcccttagc       60

t                                                                       61


<210>  3
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  single-stranded oligonucleotide 2


<220>
<221>  phosphorothioate linkage
<222>  (14)..(15)
<223>  phosphorothioate linkage

<400>  3
taagggcaac atctg                                                        15


<210>  4
<211>  26
<212>  DNA
<213>  Artificial sequence

<220>
<223>  single-stranded oligonucleotide 3


<220>
<221>  5' phosphate group
<222>  (1)..(1)
<223>  5' phosphate group

<220>
<221>  X the inverted 3' dT nucleotide
<222>  (26)..(26)
<223>  X the inverted 3' dT nucleotide

<400>  4
tcatcatcta aacccacctg tcgagt                                            26


