                         SEQUENCE LISTING

<110>  Bayer Aktiengesellschaft
 
<120>  Use of activators and stimulators of sCG comprising a beta2 
       subunit

<130>  BHC171055 FC

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  2749
<212>  DNA
<213>  Homo sapiens GUCY1B2


<220>
<221>  misc_feature
<222>  (631)..(631)
<223>  n is a, c, g, or t

<400>  1
gcttggtgct gcatctcaat ccctgtggcc ccaggagcag ctgcggcagg aacatgtatg       60

gatttatcaa cacctgtctg caatctcttg tgatagagaa atttggtgaa gaaacatggg      120

aaaaattgaa aacttctgca gaagtacaag atgccttcat gacctacaca atgtatgatg      180

acgtcatcac cattaagctc atccaagagg cctgcaacat tctgggtgtc tccatggaag      240

ccatcctgaa actctttgga gaatactttc aattctgaag atgtctggct atgacaggat      300

gctacggaca ctgggaggaa atctcatgga gtttattgaa aacctggatg ccctccacag      360

ttacctggca ctctcttatc aggagatgaa tgcaccatcg tttcgtgtgg agagaggagc      420

agatgggaaa atgtttctcc attactactc ggatagaagt ggtctgtgcc acattgtacc      480

aggtatcatt gaggctgtgg ccaaagactt ctttgatatt gatgtaatca tggacattct      540

tgacatgaat gaagaagtgg agaggacagg gaagaaggag catgttgtgt ttctgattgt      600

acagaaggct cacaggaaga tgagaaagac naagccaaaa aggttacaag acagtcaggg      660

catggagaga gaccaagagg ccctccaggc agctttcctc aagatgaagg agaaatattt      720

gaatgtctct gcttgtcctg tgaaaaaatc ccactgggat gttgtgagaa gcatagtcat      780

gtttggaaaa gggcatctca tgaacacctt tgagccaatt tatcctgaga gactctggat      840

tgaagagaag acattctgca acgcttttcc tttccacatt gtattcgatg aatcactaca      900

ggtgaagcaa gccagagtga acattcagaa gtacgtacca ggactccaaa cccagaatat      960

tcaactggat gagtatttct ccatcattca tcctcaagtt accttcaaca ttttcagcat     1020

ccgcagattt atcaacagtc aatttgtcct gaagacacga agagaaatga tgcctgtagc     1080

atggcaaagt cggactacac tcaaacttca aggccagatg atctggatgg agtccatgtg     1140

gtgcatggtg tacctgtgct ctccgaagct ccgcagcctg caagagctgg aagaactcaa     1200

tatgcatctt tctgacatcg cccccaacga caccaccagg gatctcatcc tcctgaacca     1260

gcagcggctg gctgagatag agctgtccaa ccagctggaa aggaagaagg aggagctaca     1320

ggtcctctcc aagcacttgg ccattgaaaa gaagaaaaca gagaccttac tttatgccat     1380

gctgcccaaa cacgtggcca accagctgag ggagggcaaa aaggtggctg caggagaatt     1440

taaaagctgc acgattcttt tcagcgatgt agtgacattt actaacatct gtactgcctg     1500

tgaacctata caaatagtga acgtgctgaa ttccatgtac tccaagtttg acagattaac     1560

cagtgtgcac gcagtctata aagtagaaac aataggagat gcttatatgg tggtaggagg     1620

tgtaccagtg cctattggaa accatgctca aagagtggct aattttgcct tggggatgag     1680

aatttctgca aaggaagtga cgaatcctgt tactggagaa cccatccagc tcagagtggg     1740

gatccatact ggaccagtct tagcagatgt tgtaggagac aagatgccac ggtactgctt     1800

gtttggtgac actgtgaaca cagcttctag gatggaaagt catgggcttc ccaacaaagt     1860

gcacctcagt cccacagcct acagagcctt gaaaaatcaa gggtttaaaa tcattgagag     1920

gggtgaaatt gaagtgaaag gtaaagggag aatgaccaca tactttttga tccagaactt     1980

gaatgccaca gaggatgaga tcatggggag atctaaaacc ccagttgatc acaaggggtc     2040

aacacagaaa gcatccttgc ccaccaccaa gctccagggc tcagttcaac catcttgccc     2100

tgaacactct tctcttgcat cctggttatt atgacgttct ttgagctggg ccgatagatc     2160

cagctgaaga gttctacata cattagtctt caaggttctt ctctgggcgt ttcccatgca     2220

gctctctggt agctggggaa tgatttgtaa tgagtacatg aaacacgtta aatgtccttc     2280

agcctcccta ccacgacatc caacagctgg aacttccatc cagtccttca catgatgggc     2340

acctgtggga aacctgccag gggcagccga tgttcagagg catccaaaaa aagtctccca     2400

cttcggcagc catgaagccc actcatgcaa ccgtcctcac ttttccttcc tctcacggtt     2460

ttttgcagaa gcaagtgctc agggaaatga agacaggaga actgctgccg tcatgagcta     2520

tgccgacagt cagcagctga tccccgacag aaacgcagca gatgcagacg ctcctgatga     2580

aatcatccct gtggggagtg cagggccaga tggggactcc atggagacag ccgaccggcg     2640

acagccttca gctccgacaa agacacatca ccttgcaaat gacactttgc cgtctggttt     2700

ctgtgttttg ttgtaacagg ttaaatgaac ccctgggctt aattttccc                 2749


<210>  2
<211>  617
<212>  PRT
<213>  Homo sapiens GUCY1B2

<400>  2

Met Ser Gly Tyr Asp Arg Met Leu Arg Thr Leu Gly Gly Asn Leu Met 
1               5                   10                  15      


Glu Phe Ile Glu Asn Leu Asp Ala Leu His Ser Tyr Leu Ala Leu Ser 
            20                  25                  30          


Tyr Gln Glu Met Asn Ala Pro Ser Phe Arg Val Glu Arg Gly Ala Asp 
        35                  40                  45              


Gly Lys Met Phe Leu His Tyr Tyr Ser Asp Arg Ser Gly Leu Cys His 
    50                  55                  60                  


Ile Val Pro Gly Ile Ile Glu Ala Val Ala Lys Asp Phe Phe Asp Ile 
65                  70                  75                  80  


Asp Val Ile Met Asp Ile Leu Asp Met Asn Glu Glu Val Glu Arg Thr 
                85                  90                  95      


Gly Lys Lys Glu His Val Val Phe Leu Ile Val Gln Lys Ala His Arg 
            100                 105                 110         


Lys Met Arg Lys Thr Lys Pro Lys Arg Leu Gln Asp Ser Gln Gly Met 
        115                 120                 125             


Glu Arg Asp Gln Glu Ala Leu Gln Ala Ala Phe Leu Lys Met Lys Glu 
    130                 135                 140                 


Lys Tyr Leu Asn Val Ser Ala Cys Pro Val Lys Lys Ser His Trp Asp 
145                 150                 155                 160 


Val Val Arg Ser Ile Val Met Phe Gly Lys Gly His Leu Met Asn Thr 
                165                 170                 175     


Phe Glu Pro Ile Tyr Pro Glu Arg Leu Trp Ile Glu Glu Lys Thr Phe 
            180                 185                 190         


Cys Asn Ala Phe Pro Phe His Ile Val Phe Asp Glu Ser Leu Gln Val 
        195                 200                 205             


Lys Gln Ala Arg Val Asn Ile Gln Lys Tyr Val Pro Gly Leu Gln Thr 
    210                 215                 220                 


Gln Asn Ile Gln Leu Asp Glu Tyr Phe Ser Ile Ile His Pro Gln Val 
225                 230                 235                 240 


Thr Phe Asn Ile Phe Ser Ile Arg Arg Phe Ile Asn Ser Gln Phe Val 
                245                 250                 255     


Leu Lys Thr Arg Arg Glu Met Met Pro Val Ala Trp Gln Ser Arg Thr 
            260                 265                 270         


Thr Leu Lys Leu Gln Gly Gln Met Ile Trp Met Glu Ser Met Trp Cys 
        275                 280                 285             


Met Val Tyr Leu Cys Ser Pro Lys Leu Arg Ser Leu Gln Glu Leu Glu 
    290                 295                 300                 


Glu Leu Asn Met His Leu Ser Asp Ile Ala Pro Asn Asp Thr Thr Arg 
305                 310                 315                 320 


Asp Leu Ile Leu Leu Asn Gln Gln Arg Leu Ala Glu Ile Glu Leu Ser 
                325                 330                 335     


Asn Gln Leu Glu Arg Lys Lys Glu Glu Leu Gln Val Leu Ser Lys His 
            340                 345                 350         


Leu Ala Ile Glu Lys Lys Lys Thr Glu Thr Leu Leu Tyr Ala Met Leu 
        355                 360                 365             


Pro Lys His Val Ala Asn Gln Leu Arg Glu Gly Lys Lys Val Ala Ala 
    370                 375                 380                 


Gly Glu Phe Lys Ser Cys Thr Ile Leu Phe Ser Asp Val Val Thr Phe 
385                 390                 395                 400 


Thr Asn Ile Cys Thr Ala Cys Glu Pro Ile Gln Ile Val Asn Val Leu 
                405                 410                 415     


Asn Ser Met Tyr Ser Lys Phe Asp Arg Leu Thr Ser Val His Ala Val 
            420                 425                 430         


Tyr Lys Val Glu Thr Ile Gly Asp Ala Tyr Met Val Val Gly Gly Val 
        435                 440                 445             


Pro Val Pro Ile Gly Asn His Ala Gln Arg Val Ala Asn Phe Ala Leu 
    450                 455                 460                 


Gly Met Arg Ile Ser Ala Lys Glu Val Thr Asn Pro Val Thr Gly Glu 
465                 470                 475                 480 


Pro Ile Gln Leu Arg Val Gly Ile His Thr Gly Pro Val Leu Ala Asp 
                485                 490                 495     


Val Val Gly Asp Lys Met Pro Arg Tyr Cys Leu Phe Gly Asp Thr Val 
            500                 505                 510         


Asn Thr Ala Ser Arg Met Glu Ser His Gly Leu Pro Asn Lys Val His 
        515                 520                 525             


Leu Ser Pro Thr Ala Tyr Arg Ala Leu Lys Asn Gln Gly Phe Lys Ile 
    530                 535                 540                 


Ile Glu Arg Gly Glu Ile Glu Val Lys Gly Lys Gly Arg Met Thr Thr 
545                 550                 555                 560 


Tyr Phe Leu Ile Gln Asn Leu Asn Ala Thr Glu Asp Glu Ile Met Gly 
                565                 570                 575     


Arg Ser Lys Thr Pro Val Asp His Lys Gly Ser Thr Gln Lys Ala Ser 
            580                 585                 590         


Leu Pro Thr Thr Lys Leu Gln Gly Ser Val Gln Pro Ser Cys Pro Glu 
        595                 600                 605             


His Ser Ser Leu Ala Ser Trp Leu Leu 
    610                 615         


<210>  3
<211>  1926
<212>  DNA
<213>  Homo sapiens GUCY1B1

<400>  3
atgtacggat ttgtgaatca cgccctggag ttgctggtga tccgcaatta cggccccgag       60

gtgtgggaag acatcaaaaa agaggcacag ttagatgaag aaggacagtt tcttgtcaga      120

ataatatatg atgactccaa aacttatgat ttggttgctg ctgcaagcaa agtcctcaat      180

ctcaatgctg gagaaatcct ccaaatgttt gggaagatgt ttttcgtctt ttgccaagaa      240

tctggttatg atacaatctt gcgtgtcctg ggctctaatg tcagagaatt tctacagaag      300

aatgaatcaa agcatatttc agatttacat cattgtttcc tgggaatcta cttgtgctgc      360

aataaccttg atgctctgca cgaccacctt gctaccatct acccaggaat gcgtgcacct      420

tcctttaggt gcactgatgc agaaaagggc aaaggactca ttttgcacta ctactcagag      480

agagaaggac ttcaggatat tgtcattgga atcatcaaaa cagtggcaca acaaatccat      540

ggcactgaaa tagacatgaa ggttattcag caaagaaatg aagaatgtga tcatactcaa      600

tttttaattg aagaaaaaga gtcaaaagaa gaggattttt atgaagatct tgacagattt      660

gaagaaaatg gtacccagga atcacgcatc agcccatata cattctgcaa agcttttcct      720

tttcatataa tatttgaccg ggacctagtg gtcactcagt gtggcaatgc tatatacaga      780

gttctccccc agctccagcc tgggaattgc agccttctgt ctgtcttctc gctggttcgt      840

cctcatattg atattagttt ccatgggatc ctttctcaca tcaatactgt ttttgtattg      900

agaagcaagg aaggattgtt ggatgtggag aaattagaat gtgaggatga actgactggg      960

actgagatca gctgcttacg tctcaagggt caaatgatct acttacctga agcagatagc     1020

atactttttc tatgttcacc aagtgtcatg aacctggacg atttgacaag gagagggctg     1080

tatctaagtg acatccctct gcatgatgcc acgcgcgatc ttgttctttt gggagaacaa     1140

tttagagagg aatacaaact cacccaagaa ctggaaatcc tcactgacag gctacagctc     1200

acgttaagag ccctggaaga tgaaaagaaa aagacagaca cattgctgta ttctgtcctt     1260

cctccgtctg ttgccaatga gctgcggcac aagcgtccag tgcctgccaa aagatatgac     1320

aatgtgacca tcctctttag tggcattgtg ggcttcaatg ctttctgtag caagcatgca     1380

tctggagaag gagccatgaa gatcgtcaac ctcctcaacg acctctacac cagatttgac     1440

acactgactg attcccggaa aaacccattt gtttataagg tggagactgt tggtgacaag     1500

tatatgacag tgagtggttt accagagcca tgcattcacc atgcacgatc catctgccac     1560

ctggccttgg acatgatgga aattgctggc caggttcaag tagatggtga atctgttcag     1620

ataacaatag ggatacacac tggagaggta gttacaggtg tcataggaca gcggatgcct     1680

cgatactgtc tttttgggaa tactgtcaac ctcacaagcc gaacagaaac cacaggagaa     1740

aagggaaaaa taaatgtgtc tgaatataca tacagatgtc ttatgtctcc agaaaattca     1800

gatccacaat tccacttgga gcacagaggc ccagtgtcca tgaagggcaa aaaagaacca     1860

atgcaagttt ggtttctatc cagaaaaaat acaggaacag aggaaacaaa gcaggatgat     1920

gactga                                                                1926


<210>  4
<211>  641
<212>  PRT
<213>  Homo sapiens GUCY1B1

<400>  4

Met Tyr Gly Phe Val Asn His Ala Leu Glu Leu Leu Val Ile Arg Asn 
1               5                   10                  15      


Tyr Gly Pro Glu Val Trp Glu Asp Ile Lys Lys Glu Ala Gln Leu Asp 
            20                  25                  30          


Glu Glu Gly Gln Phe Leu Val Arg Ile Ile Tyr Asp Asp Ser Lys Thr 
        35                  40                  45              


Tyr Asp Leu Val Ala Ala Ala Ser Lys Val Leu Asn Leu Asn Ala Gly 
    50                  55                  60                  


Glu Ile Leu Gln Met Phe Gly Lys Met Phe Phe Val Phe Cys Gln Glu 
65                  70                  75                  80  


Ser Gly Tyr Asp Thr Ile Leu Arg Val Leu Gly Ser Asn Val Arg Glu 
                85                  90                  95      


Phe Leu Gln Lys Asn Glu Ser Lys His Ile Ser Asp Leu His His Cys 
            100                 105                 110         


Phe Leu Gly Ile Tyr Leu Cys Cys Asn Asn Leu Asp Ala Leu His Asp 
        115                 120                 125             


His Leu Ala Thr Ile Tyr Pro Gly Met Arg Ala Pro Ser Phe Arg Cys 
    130                 135                 140                 


Thr Asp Ala Glu Lys Gly Lys Gly Leu Ile Leu His Tyr Tyr Ser Glu 
145                 150                 155                 160 


Arg Glu Gly Leu Gln Asp Ile Val Ile Gly Ile Ile Lys Thr Val Ala 
                165                 170                 175     


Gln Gln Ile His Gly Thr Glu Ile Asp Met Lys Val Ile Gln Gln Arg 
            180                 185                 190         


Asn Glu Glu Cys Asp His Thr Gln Phe Leu Ile Glu Glu Lys Glu Ser 
        195                 200                 205             


Lys Glu Glu Asp Phe Tyr Glu Asp Leu Asp Arg Phe Glu Glu Asn Gly 
    210                 215                 220                 


Thr Gln Glu Ser Arg Ile Ser Pro Tyr Thr Phe Cys Lys Ala Phe Pro 
225                 230                 235                 240 


Phe His Ile Ile Phe Asp Arg Asp Leu Val Val Thr Gln Cys Gly Asn 
                245                 250                 255     


Ala Ile Tyr Arg Val Leu Pro Gln Leu Gln Pro Gly Asn Cys Ser Leu 
            260                 265                 270         


Leu Ser Val Phe Ser Leu Val Arg Pro His Ile Asp Ile Ser Phe His 
        275                 280                 285             


Gly Ile Leu Ser His Ile Asn Thr Val Phe Val Leu Arg Ser Lys Glu 
    290                 295                 300                 


Gly Leu Leu Asp Val Glu Lys Leu Glu Cys Glu Asp Glu Leu Thr Gly 
305                 310                 315                 320 


Thr Glu Ile Ser Cys Leu Arg Leu Lys Gly Gln Met Ile Tyr Leu Pro 
                325                 330                 335     


Glu Ala Asp Ser Ile Leu Phe Leu Cys Ser Pro Ser Val Met Asn Leu 
            340                 345                 350         


Asp Asp Leu Thr Arg Arg Gly Leu Tyr Leu Ser Asp Ile Pro Leu His 
        355                 360                 365             


Asp Ala Thr Arg Asp Leu Val Leu Leu Gly Glu Gln Phe Arg Glu Glu 
    370                 375                 380                 


Tyr Lys Leu Thr Gln Glu Leu Glu Ile Leu Thr Asp Arg Leu Gln Leu 
385                 390                 395                 400 


Thr Leu Arg Ala Leu Glu Asp Glu Lys Lys Lys Thr Asp Thr Leu Leu 
                405                 410                 415     


Tyr Ser Val Leu Pro Pro Ser Val Ala Asn Glu Leu Arg His Lys Arg 
            420                 425                 430         


Pro Val Pro Ala Lys Arg Tyr Asp Asn Val Thr Ile Leu Phe Ser Gly 
        435                 440                 445             


Ile Val Gly Phe Asn Ala Phe Cys Ser Lys His Ala Ser Gly Glu Gly 
    450                 455                 460                 


Ala Met Lys Ile Val Asn Leu Leu Asn Asp Leu Tyr Thr Arg Phe Asp 
465                 470                 475                 480 


Thr Leu Thr Asp Ser Arg Lys Asn Pro Phe Val Tyr Lys Val Glu Thr 
                485                 490                 495     


Val Gly Asp Lys Tyr Met Thr Val Ser Gly Leu Pro Glu Pro Cys Ile 
            500                 505                 510         


His His Ala Arg Ser Ile Cys His Leu Ala Leu Asp Met Met Glu Ile 
        515                 520                 525             


Ala Gly Gln Val Gln Val Asp Gly Glu Ser Val Gln Ile Thr Ile Gly 
    530                 535                 540                 


Ile His Thr Gly Glu Val Val Thr Gly Val Ile Gly Gln Arg Met Pro 
545                 550                 555                 560 


Arg Tyr Cys Leu Phe Gly Asn Thr Val Asn Leu Thr Ser Arg Thr Glu 
                565                 570                 575     


Thr Thr Gly Glu Lys Gly Lys Ile Asn Val Ser Glu Tyr Thr Tyr Arg 
            580                 585                 590         


Cys Leu Met Ser Pro Glu Asn Ser Asp Pro Gln Phe His Leu Glu His 
        595                 600                 605             


Arg Gly Pro Val Ser Met Lys Gly Lys Lys Glu Pro Met Gln Val Trp 
    610                 615                 620                 


Phe Leu Ser Arg Lys Asn Thr Gly Thr Glu Glu Thr Lys Gln Asp Asp 
625                 630                 635                 640 


Asp 
    


<210>  5
<211>  408
<212>  DNA
<213>  Homo sapiens RpL32

<400>  5
atggccgccc tcagacccct tgtgaagccc aagatcgtca aaaagagaac caagaagttc       60

atccggcacc agtcagaccg atatgtcaaa attaagcgta actggcggaa acccagaggc      120

attgacaaca gggttcgtag aagattcaag ggccagatct tgatgcccaa cattggttat      180

ggaagcaaca aaaaaacaaa gcacatgctg cccagtggct tccggaagtt cctggtccac      240

aacgtcaagg agctggaagt gctgctgatg tgcaacaaat cttactgtgc cgagatcgct      300

cacaatgttt cctccaagaa ccgcaaagcc atcgtggaaa gagctgccca actggccatc      360

agagtcacca accccaatgc caggctgcgc agtgaagaaa atgagtag                   408


<210>  6
<211>  135
<212>  PRT
<213>  Homo sapiens RpL32

<400>  6

Met Ala Ala Leu Arg Pro Leu Val Lys Pro Lys Ile Val Lys Lys Arg 
1               5                   10                  15      


Thr Lys Lys Phe Ile Arg His Gln Ser Asp Arg Tyr Val Lys Ile Lys 
            20                  25                  30          


Arg Asn Trp Arg Lys Pro Arg Gly Ile Asp Asn Arg Val Arg Arg Arg 
        35                  40                  45              


Phe Lys Gly Gln Ile Leu Met Pro Asn Ile Gly Tyr Gly Ser Asn Lys 
    50                  55                  60                  


Lys Thr Lys His Met Leu Pro Ser Gly Phe Arg Lys Phe Leu Val His 
65                  70                  75                  80  


Asn Val Lys Glu Leu Glu Val Leu Leu Met Cys Asn Lys Ser Tyr Cys 
                85                  90                  95      


Ala Glu Ile Ala His Asn Val Ser Ser Lys Asn Arg Lys Ala Ile Val 
            100                 105                 110         


Glu Arg Ala Ala Gln Leu Ala Ile Arg Val Thr Asn Pro Asn Ala Arg 
        115                 120                 125             


Leu Arg Ser Glu Glu Asn Glu 
    130                 135 


<210>  7
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer_GUCY1B1_forward

<400>  7
tatacataca ggtgtctcat gtc                                               23


<210>  8
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer_GUCY1B1_reverse

<400>  8
ggacacagga cctctgtg                                                     18


<210>  9
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer_GUCY1B1_TaqManprobe

<400>  9
ccagaaaact cggatccaca gttccactt                                         29


<210>  10
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer_GUCY1B2_forward

<400>  10
cacagagccc tgaaaaacaa ag                                                22


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer_GUCY1B2_reverse

<400>  11
gtggtcatct ttcctttccc                                                   20


<210>  12
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer_GUCY1B2_TaqManprobe

<400>  12
ttgaaattgt caggagaggc gagatcgaag tg                                     32


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer_RpL32_forward

<400>  13
gaaagagcag cacagctggc                                                   20


<210>  14
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer_RpL32_reverse

<400>  14
tcattctctt cgctgcgtag c                                                 21


<210>  15
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer_RpL32_TaqManprobe

<400>  15
tcagagtcac caatcccaac gcca                                              24


