                         б

<110>  ż򣨱Ƽ޹˾
 
<120>  һ̥Ѫ쵰ױķ

<130>  FB00066PCT

<150>  CN201711027708.6
<151>  2017-10-27

<160>  63    

<170>  PatentIn version 3.5

<210>  1
<211>  717
<212>  DNA
<213>  ˹

<220>
<223>  GFP mRNAϢ

<400>  1
atgagtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt       60

gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga      120

aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt      180

gtcactactt tctcttatgg tgttcaatgc ttttcaagat acccagatca tatgaaacgg      240

catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc      300

aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt      360

aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa      420

ttggaataca actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga      480

atcaaagtta acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac      540

cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac      600

ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt      660

cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata caaatag         717


<210>  2
<211>  150
<212>  DNA
<213>  ˹

<220>
<223>  CL11A enhancer 58Kλ150bp

<400>  2
ctgccagtcc tcttctaccc cacccacgcc cccaccctaa tcagaggcca aacccttcct       60

ggagcctgtg ataaaagcaa ctgttagctt gcactagact agcttcaaag ttgtattgac      120

cctggtgtgt tatgtctaag agtagatgcc                                       150


<210>  3
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-1sgRNA(ʱҲΪenhancer-1)

<400>  3
cacaggctcc aggaagggtt                                                   20


<210>  4
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-2sgRNA(ʱҲΪenhancer-2)

<400>  4
atcagaggcc aaacccttcc                                                   20


<210>  5
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-3sgRNA(ʱҲΪenhancer-3)

<400>  5
ctaacagttg cttttatcac                                                   20


<210>  6
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-4sgRNA(ʱҲΪenhancer-4)

<400>  6
ttgcttttat cacaggctcc                                                   20


<210>  7
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-5sgRNA(ʱҲΪenhancer-5)

<400>  7
ttttatcaca ggctccagga                                                   20


<210>  8
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-6sgRNA(ʱҲΪenhancer-6)

<400>  8
tttatcacag gctccaggaa                                                   20


<210>  9
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-7sgRNA(ʱҲΪenhancer-7)

<400>  9
tgggtggggt agaagaggac                                                   20


<210>  10
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-8sgRNA(ʱҲΪenhancer-8)

<400>  10
gggcgtgggt ggggtagaag                                                   20


<210>  11
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-9sgRNA(ʱҲΪenhancer-9)

<400>  11
ttagggtggg ggcgtgggtg                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-10sgRNA(ʱҲΪenhancer-10)

<400>  12
attagggtgg gggcgtgggt                                                   20


<210>  13
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-11sgRNA(ʱҲΪenhancer-11)

<400>  13
gattagggtg ggggcgtggg                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-12sgRNA(ʱҲΪenhancer-12)

<400>  14
tctgattagg gtgggggcgt                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-13sgRNA(ʱҲΪenhancer-13)

<400>  15
ctctgattag ggtgggggcg                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-14sgRNA(ʱҲΪenhancer-14)

<400>  16
cacgccccca ccctaatcag                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-15sgRNA(ʱҲΪenhancer-15)

<400>  17
ttggcctctg attagggtgg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-16sgRNA(ʱҲΪenhancer-16)

<400>  18
tttggcctct gattagggtg                                                   20


<210>  19
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-17sgRNA(ʱҲΪenhancer-17)

<400>  19
gtttggcctc tgattagggt                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-18sgRNA(ʱҲΪenhancer-18)

<400>  20
ggtttggcct ctgattaggg                                                   20


<210>  21
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-19sgRNA(ʱҲΪenhancer-19)

<400>  21
aagggtttgg cctctgatta                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-20sgRNA(ʱҲΪenhancer-20)

<400>  22
gaagggtttg gcctctgatt                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-21sgRNA(ʱҲΪenhancer-21)

<400>  23
actcttagac ataacacacc                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-22sgRNA(ʱҲΪenhancer-22)

<400>  24
cttcaaagtt gtattgaccc                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  ΪBCL11Aǿ-23sgRNA(ʱҲΪenhancer-23)

<400>  25
ctcttagaca taacacacca                                                   20


<210>  26
<211>  4101
<212>  DNA
<213>  ˹

<220>
<223>  Cas9 mRNA

<400>  26
gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc       60

accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac      120

agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc      180

acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat      240

ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg      300

gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac      360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa      420

ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg      480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg      540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc      600

aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg      660

ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg      720

attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat      780

gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag      840

atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg      900

ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg      960

atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag     1020

cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc     1080

tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa     1140

aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag     1200

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc     1260

attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag     1320

aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga     1380

ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg     1440

gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac     1500

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat     1560

aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc     1620

ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg     1680

aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc     1740

ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc     1800

aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg     1860

accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac     1920

ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg     1980

ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat     2040

ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc     2100

ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac     2160

gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg     2220

aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc     2280

gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg     2340

aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg     2400

gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat     2460

atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc     2520

gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac     2580

aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac     2640

tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc     2700

aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg     2760

gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact     2820

aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag     2880

ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac     2940

caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac     3000

cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg     3060

atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac     3120

atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct     3180

ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc     3240

accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag     3300

acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc     3360

agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat     3420

tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa     3480

gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt     3540

ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac     3600

tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag     3660

aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac     3720

tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag     3780

cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc     3840

ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc     3900

atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct     3960

gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag     4020

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac     4080

ctgtctcagc tgggaggcga c                                               4101


<210>  27
<211>  24
<212>  DNA
<213>  ˹

<220>
<223>  

<400>  27
cacctcagca gaaacaaagt tatc                                              24


<210>  28
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  

<400>  28
gggaagctcc aaactctcaa                                                   20


<210>  29
<211>  24
<212>  DNA
<213>  ˹

<220>
<223>  У

<400>  29
cacctcagca gaaacaaagt tatc                                              24


<210>  30
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  У

<400>  30
gggaagctcc aaactctcaa                                                   20


<210>  31
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  chr2:60495236chr2:60495271֮ĺ

<400>  31
atcagaggcc aaacccttcc tggagcctgt gataaa                                 36


<210>  32
<211>  36
<212>  DNA
<213>  ˹

<220>
<223>  chr2:60495236chr2:60495271֮ĺеĻ

<400>  32
tttatcacag gctccaggaa gggtttggcc tctgat                                 36


<210>  33
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      
<400>  33
tcagaggcca aacccttcct                                                   20


<210>  34
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       
<400>  34
cagaggccaa acccttcctg                                                   20


<210>  35
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      
<400>  35
agaggccaaa cccttcctgg                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      
<400>  36
gaggccaaac ccttcctgga                                                   20


<210>  37
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
 

<400>  37
aggccaaacc cttcctggag                                                   20


<210>  38
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       
<400>  38
ggccaaaccc ttcctggagc                                                   20


<210>  39
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      

<400>  39
gccaaaccct tcctggagcc                                                   20


<210>  40
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  40
ccaaaccctt cctggagcct                                                   20


<210>  41
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  41
caaacccttc ctggagcctg                                                   20


<210>  42
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  42
aaacccttcc tggagcctgt                                                   20


<210>  43
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      

<400>  43
aacccttcct ggagcctgtg                                                   20


<210>  44
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      

<400>  44
acccttcctg gagcctgtga                                                   20


<210>  45
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      

<400>  45
cccttcctgg agcctgtgat                                                   20


<210>  46
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  46
ccttcctgga gcctgtgata                                                   20


<210>  47
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  47
cttcctggag cctgtgataa                                                   20


<210>  48
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      

<400>  48
ttcctggagc ctgtgataaa                                                   20


<210>  49
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      

<400>  49
ttatcacagg ctccaggaag                                                   20


<210>  50
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  50
tatcacaggc tccaggaagg                                                   20


<210>  51
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  51
atcacaggct ccaggaaggg                                                   20


<210>  52
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  52
tcacaggctc caggaagggt                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  53
acaggctcca ggaagggttt                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  54
caggctccag gaagggtttg                                                   20


<210>  55
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      

<400>  55
aggctccagg aagggtttgg                                                   20


<210>  56
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      

<400>  56
ggctccagga agggtttggc                                                   20


<210>  57
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA

      
<400>  57
gctccaggaa gggtttggcc                                                   20


<210>  58
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  58
ctccaggaag ggtttggcct                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  59
tccaggaagg gtttggcctc                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      

<400>  60
ccaggaaggg tttggcctct                                                   20


<210>  61
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  61
caggaagggt ttggcctctg                                                   20


<210>  62
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
      

<400>  62
aggaagggtt tggcctctga                                                   20


<210>  63
<211>  20
<212>  DNA
<213>  ˹

<220>
<223>  CTTCCT0-15CTTCCT0-15BCL11AsgRNA
       

<400>  63
ggaagggttt ggcctctgat                                                   20


