                         SEQUENCE LISTING

<110>  Centro de Investigaciones Energeticas, Medioambientales y 
       Tecnologicas O.A, M.P.
       Fundacion Instituto de Investigacion Sanitaria Fundacion 
       Jimenez Diaz
       Centro de Investigacion Biomedica en Red
 
<120>  LENTIVIRAL VECTORS FOR DELIVERY OF PKLR TO TREAT PYRUVATE KINASE 
       DEFICIENCY

<130>  ROPA-004/01US 329592-2050

<150>  US 62/573,037
<151>  2017-10-16

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Rev responsive element (RRE)

<400>  1
aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat       60

gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt      120

gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca      180

gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcct            234


<210>  2
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 packaging signal (psi)

<400>  2
ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga       60

ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag      120

agcgtc                                                                 126


<210>  3
<211>  181
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 5' LTR

<400>  3
gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca       60

ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg      120

tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc      180

a                                                                      181


<210>  4
<211>  234
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HIV-1 self-inactivating 3' LTR

<400>  4
tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc       60

tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta      120

agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact      180

ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agca            234


<210>  5
<211>  204
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human cytomegalovirus (CMV) immediate early promoter

<400>  5
gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt       60

ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac      120

tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg      180

tgggaggtct atataagcag agct                                             204


<210>  6
<211>  118
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  central polypurine tract and central termination sequence of 
       HIV-1 (cPPT/CTS)

<400>  6
ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat       60

agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaatttt        118


<210>  7
<211>  511
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human phosphoglycerate kinase 1 (hPGK) promoter

<400>  7
ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc       60

tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc      120

cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc      180

tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac      240

ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc      300

gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag      360

cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct      420

gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct      480

cgttgaccga atcaccgacc tctctcccca g                                     511


<210>  8
<211>  1725
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  codon-optimized version of the human PKLR cDNA (coRPK)

<400>  8
atgagcatcc aggaaaatat cagctctctg cagctgcggt cctgggtgtc caagagccag       60

agagacctgg ccaagagcat cctgatcgga gcccctggcg gaccagccgg atacctgaga      120

agggctagcg tggcccagct gacccaggaa ctgggcaccg cctttttcca gcagcagcag      180

ctgccagccg ccatggccga cacctttctg gaacacctgt gcctgctgga catcgactct      240

gagcccgtgg ccgccagaag caccagcatc attgccacca tcggccctgc cagcagaagc      300

gtggagcggc tgaaagagat gatcaaggcc ggcatgaata tcgcccggct gaacttctcc      360

cacggcagcc acgagtacca cgcagagagc attgccaacg tccgggaggc cgtggagagc      420

tttgccggca gccccctgag ctacagaccc gtggccattg ccctggacac caagggcccc      480

gagatcagaa caggaattct gcagggaggg cctgagagcg aggtggagct ggtgaagggc      540

agccaagtgc tggtgaccgt ggaccccgcc ttcagaacca gaggcaacgc caacacagtg      600

tgggtggact accccaacat cgtgcgggtg gtgcctgtgg gcggcagaat ctacatcgac      660

gacggcctga tcagcctggt ggtgcagaag atcggacctg agggcctggt gacccaggtc      720

gagaatggcg gcgtgctggg cagcagaaag ggcgtgaatc tgccaggcgc ccaggtggac      780

ctgcctggcc tgtctgagca ggacgtgaga gacctgagat ttggcgtgga gcacggcgtg      840

gacatcgtgt tcgccagctt cgtgcggaag gcctctgatg tggccgccgt gagagccgct      900

ctgggccctg aaggccacgg catcaagatc atcagcaaga tcgagaacca cgagggcgtg      960

aagcggttcg acgagatcct ggaagtgtcc gacggcatca tggtggccag aggcgacctg     1020

ggcatcgaga tccccgccga gaaggtgttc ctggcccaga aaatgatgat cggacggtgc     1080

aacctggccg gcaaacctgt ggtgtgcgcc acccagatgc tggaaagcat gatcaccaag     1140

cccagaccca ccagagccga gacaagcgac gtggccaacg ccgtgctgga tggcgctgac     1200

tgcatcatgc tgtccggcga gacagccaag ggcaacttcc ccgtggaggc cgtgaagatg     1260

cagcacgcca ttgccagaga agccgaggcc gccgtgtacc accggcagct gttcgaggaa     1320

ctgcggagag ccgcccctct gagcagagat cccaccgaag tgaccgccat cggagccgtg     1380

gaagccgcct tcaagtgctg cgccgctgca atcatcgtgc tgaccaccac aggcagaagc     1440

gcccagctgc tgtccagata cagacccaga gccgccgtga tcgccgtgac aagatccgcc     1500

caggccgcta gacaggtcca cctgtgcaga ggcgtgttcc ccctgctgta ccgggagcct     1560

cccgaggcca tctgggccga cgacgtggac agacgggtgc agttcggcat cgagagcggc     1620

aagctgcggg gcttcctgag agtgggcgac ctggtgatcg tggtgacagg ctggcggcct     1680

ggcagcggct acaccaacat catgagggtg ctgtccatca gctga                     1725


<210>  9
<211>  380
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human CMV enhancer

<400>  9
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg                                                  380


<210>  10
<211>  122
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  simian virus 40 (SV40) poly(A) signal

<400>  10
aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca       60

aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct      120

ta                                                                     122


<210>  11
<211>  136
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SV40 origin of replication

<400>  11
atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt       60

tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga      120

ggcttttttg gaggcc                                                      136


<210>  12
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  central polypurine tract (cPPT)

<400>  12
tttaaaagaa aaggggggat tggggggt                                          28


<210>  13
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  dNEF signal sequence

<400>  13
gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca       60

ctttttaaaa gaaaaggggg gac                                               83


<210>  14
<211>  795
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NeoR/KanR sequence

<400>  14
atgattgaac aagatggatt gcacgcaggt tctccggcgg cttgggtgga gaggctattc       60

ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca      120

gcgcaggggc gtccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg      180

caagacgagg cagcgcggct atcgtggctg gcgacgacgg gcgttccttg cgcggctgtg      240

ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag      300

gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg      360

cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc      420

atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa      480

gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgtc tatgcccgac      540

ggcgaggatc tcgtcgtgac ccacggcgat gcctgcttgc cgaatatcat ggtggaaaat      600

ggccgctttt ctggattcat cgactgtggc cgtctgggtg tggcggaccg ctatcaggac      660

atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc      720

cttgtgcttt acggtatcgc cgcgcccgat tcgcagcgca tcgccttcta tcgccttctt      780

gacgagttct tctga                                                       795


<210>  15
<211>  137
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rrnG terminator (transcription terminator from the E.coli 
       ribosomal RNA rrnG operon)

<400>  15
gcattggcgc agaaaaaaat gcctgatgcg acgctgcgcg tcttatactc ccacatatgc       60

cagattcagc aacggatacg gcttccccaa cttgcccact tccatacgtg tcctccttac      120

cagaaattta tccttaa                                                     137


<210>  16
<211>  589
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ori (high-copy-number ColE1/pMB1/pBR322/pUC origin of 
       replication)

<400>  16
ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc       60

agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt      120

cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt      180

caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc      240

tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa      300

ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac      360

ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg      420

gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga      480

gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact      540

tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaa                  589


<210>  17
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CAP binding site sequence

<400>  17
taatgtgagt tagctcactc at                                                22


<210>  18
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E.coli lac promoter

<400>  18
tttacacttt atgcttccgg ctcgtatgtt g                                      31


<210>  19
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  lac operator sequence

<400>  19
ttgtgagcgg ataacaa                                                      17


<210>  20
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T3 promoter (promoter for bacteriophage T3 RNA polymerase)

<400>  20
aattaaccct cactaaagg                                                    19


<210>  21
<211>  380
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CMV enhancer

<400>  21
gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc       60

catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca      120

acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga      180

ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc      240

aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct      300

ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat      360

tagtcatcgc tattaccatg                                                  380


<210>  22
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  T7 promoter (promoter for bacteriophage T7 RNA polymerase)

<400>  22
cctatagtga gtcgtatta                                                    19


<210>  23
<211>  429
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  f1 ori (f1 bacteriophage origin of replication)

<400>  23
acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg       60

ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca      120

cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta      180

gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc      240

catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg      300

gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat      360

aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta      420

acgcgaatt                                                              429


<210>  24
<211>  677
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  chimeric wPRE RNA export signal

<400>  24
cgagcatctt accgccattt attcccatat ttgttctgtt tttcttgatt tgggtataca       60

tttaaatgtt aataaaacaa aatggtgggg caatcattta catttttagg gatatgtaat      120

tactagttca ggtgtattgc cacaagacaa acatgttaag aaactttccc gttatttacg      180

ctctgttcct gttaatcaac ctctggatta caaaatttgt gaaagattga ctgatattct      240

taactatgtt gctcctttta cgctgtgtgg atatgctgct ttaatgcctc tgtatcatgc      300

tattgcttcc cgtacggctt tcgttttctc ctccttgtat aaatcctggt tgctgtctct      360

ttatgaggag ttgtggcccg ttgtccgtca acgtggcgtg gtgtgctctg tgtttgctga      420

cgcaaccccc actggctggg gcattgccac cacctgtcaa ctcctttctg ggactttcgc      480

tttccccctc ccgatcgcca cggcagaact catcgccgcc tgccttgccc gctgctggac      540

aggggctagg ttgctgggca ctgataattc cgtggtgttg tcggggaagg gcctgctgcc      600

ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg      660

ggccgcctcc ccgcctg                                                     677


<210>  25
<211>  9087
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  self-limiting lentivirus gene delivery vector

<400>  25
gtgtttaaac ctagatattg atagtctgat cggtcaacgt ataatcgagt cctagctttt       60

gcaaacatct atcaagagac aggatcagca ggaggctttc gcatgattga acaagatgga      120

ttgcacgcag gttctccggc ggcttgggtg gagaggctat tcggctatga ctgggcacaa      180

cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgtccggtt      240

ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg      300

ctatcgtggc tggcgacgac gggcgttcct tgcgcggctg tgctcgacgt tgtcactgaa      360

gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac      420

cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt      480

gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact      540

cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg      600

ccagccgaac tgttcgccag gctcaaggcg tctatgcccg acggcgagga tctcgtcgtg      660

acccacggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc      720

atcgactgtg gccgtctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt      780

gatattgctg aagagcttgg cggcgaatgg gctgaccgct tccttgtgct ttacggtatc      840

gccgcgcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaccg      900

attctaggtg cattggcgca gaaaaaaatg cctgatgcga cgctgcgcgt cttatactcc      960

cacatatgcc agattcagca acggatacgg cttccccaac ttgcccactt ccatacgtgt     1020

cctccttacc agaaatttat ccttaacgat cggacgggga gtcaggcaac tatggatgaa     1080

cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac     1140

caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc     1200

taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc     1260

cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg     1320

cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg     1380

gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca     1440

aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg     1500

cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg     1560

tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga     1620

acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac     1680

ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat     1740

ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc     1800

tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga     1860

tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc     1920

ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg     1980

gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag     2040

cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc     2100

gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc     2160

agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac     2220

tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga     2280

aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag     2340

ctggagctgc aagcttggcc attgcatacg ttgtatccat atcataatat gtacatttat     2400

attggctcat gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag     2460

taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt     2520

acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg     2580

acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat     2640

ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct     2700

attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg     2760

gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg     2820

ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc     2880

caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa     2940

tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc     3000

tatataagca gagctcgttt agtgaaccgg ggtctctctg gttagaccag atctgagcct     3060

gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag     3120

tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac     3180

ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa     3240

agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag     3300

aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga     3360

gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa     3420

aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag     3480

cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag     3540

acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt     3600

atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa     3660

ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc     3720

ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat     3780

ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa     3840

gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg     3900

gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat     3960

tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc     4020

atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg     4080

aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt     4140

gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga     4200

atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact     4260

ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag     4320

ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat     4380

tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta     4440

tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc     4500

cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca     4560

gatccattcg attagtgaac ggatctcgac ggtatcggtt aacttttaaa agaaaagggg     4620

ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa     4680

ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tatcgatcac gagactagcc     4740

tcgagaagct tgatatcgaa ttccacgggg ttggggttgc gccttttcca aggcagccct     4800

gggtttgcgc agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac     4860

cctgggtctc gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc     4920

cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg     4980

ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac     5040

ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc     5100

tgctcagcag ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt     5160

ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg     5220

gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg     5280

gatccgtcga caccggtgcc accatgagca tccaggaaaa tatcagctct ctgcagctgc     5340

ggtcctgggt gtccaagagc cagagagacc tggccaagag catcctgatc ggagcccctg     5400

gcggaccagc cggatacctg agaagggcta gcgtggccca gctgacccag gaactgggca     5460

ccgccttttt ccagcagcag cagctgccag ccgccatggc cgacaccttt ctggaacacc     5520

tgtgcctgct ggacatcgac tctgagcccg tggccgccag aagcaccagc atcattgcca     5580

ccatcggccc tgccagcaga agcgtggagc ggctgaaaga gatgatcaag gccggcatga     5640

atatcgcccg gctgaacttc tcccacggca gccacgagta ccacgcagag agcattgcca     5700

acgtccggga ggccgtggag agctttgccg gcagccccct gagctacaga cccgtggcca     5760

ttgccctgga caccaagggc cccgagatca gaacaggaat tctgcaggga gggcctgaga     5820

gcgaggtgga gctggtgaag ggcagccaag tgctggtgac cgtggacccc gccttcagaa     5880

ccagaggcaa cgccaacaca gtgtgggtgg actaccccaa catcgtgcgg gtggtgcctg     5940

tgggcggcag aatctacatc gacgacggcc tgatcagcct ggtggtgcag aagatcggac     6000

ctgagggcct ggtgacccag gtcgagaatg gcggcgtgct gggcagcaga aagggcgtga     6060

atctgccagg cgcccaggtg gacctgcctg gcctgtctga gcaggacgtg agagacctga     6120

gatttggcgt ggagcacggc gtggacatcg tgttcgccag cttcgtgcgg aaggcctctg     6180

atgtggccgc cgtgagagcc gctctgggcc ctgaaggcca cggcatcaag atcatcagca     6240

agatcgagaa ccacgagggc gtgaagcggt tcgacgagat cctggaagtg tccgacggca     6300

tcatggtggc cagaggcgac ctgggcatcg agatccccgc cgagaaggtg ttcctggccc     6360

agaaaatgat gatcggacgg tgcaacctgg ccggcaaacc tgtggtgtgc gccacccaga     6420

tgctggaaag catgatcacc aagcccagac ccaccagagc cgagacaagc gacgtggcca     6480

acgccgtgct ggatggcgct gactgcatca tgctgtccgg cgagacagcc aagggcaact     6540

tccccgtgga ggccgtgaag atgcagcacg ccattgccag agaagccgag gccgccgtgt     6600

accaccggca gctgttcgag gaactgcgga gagccgcccc tctgagcaga gatcccaccg     6660

aagtgaccgc catcggagcc gtggaagccg ccttcaagtg ctgcgccgct gcaatcatcg     6720

tgctgaccac cacaggcaga agcgcccagc tgctgtccag atacagaccc agagccgccg     6780

tgatcgccgt gacaagatcc gcccaggccg ctagacaggt ccacctgtgc agaggcgtgt     6840

tccccctgct gtaccgggag cctcccgagg ccatctgggc cgacgacgtg gacagacggg     6900

tgcagttcgg catcgagagc ggcaagctgc ggggcttcct gagagtgggc gacctggtga     6960

tcgtggtgac aggctggcgg cctggcagcg gctacaccaa catcatgagg gtgctgtcca     7020

tcagctgacc gcggtctaga ggatcccccg ggctgcagga attcgagcat cttaccgcca     7080

tttattccca tatttgttct gtttttcttg atttgggtat acatttaaat gttaataaaa     7140

caaaatggtg gggcaatcat ttacattttt agggatatgt aattactagt tcaggtgtat     7200

tgccacaaga caaacatgtt aagaaacttt cccgttattt acgctctgtt cctgttaatc     7260

aacctctgga ttacaaaatt tgtgaaagat tgactgatat tcttaactat gttgctcctt     7320

ttacgctgtg tggatatgct gctttaatgc ctctgtatca tgctattgct tcccgtacgg     7380

ctttcgtttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc     7440

ccgttgtccg tcaacgtggc gtggtgtgct ctgtgtttgc tgacgcaacc cccactggct     7500

ggggcattgc caccacctgt caactccttt ctgggacttt cgctttcccc ctcccgatcg     7560

ccacggcaga actcatcgcc gcctgccttg cccgctgctg gacaggggct aggttgctgg     7620

gcactgataa ttccgtggtg ttgtcgggga agggcctgct gccggctctg cggcctcttc     7680

cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg     7740

gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca     7800

ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct     7860

gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg     7920

ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt     7980

gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt     8040

gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc     8100

aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa     8160

ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg     8220

tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta     8280

actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga     8340

ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag     8400

tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt     8460

attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta     8520

cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg     8580

cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct     8640

gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg     8700

ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg     8760

gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac     8820

ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct     8880

gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt     8940

tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt     9000

tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt     9060

ttaacaaaat cgttccctca ggacgtc                                         9087


<210>  26
<211>  2890
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PKD Expression Cassette Sequence (5`-3`)

<400>  26
ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc       60

tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc      120

cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc      180

tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac      240

ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc      300

gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag      360

cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct      420

gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct      480

cgttgaccga atcaccgacc tctctcccca gggggatccg tcgacaccgg tgccaccatg      540

agcatccagg aaaatatcag ctctctgcag ctgcggtcct gggtgtccaa gagccagaga      600

gacctggcca agagcatcct gatcggagcc cctggcggac cagccggata cctgagaagg      660

gctagcgtgg cccagctgac ccaggaactg ggcaccgcct ttttccagca gcagcagctg      720

ccagccgcca tggccgacac ctttctggaa cacctgtgcc tgctggacat cgactctgag      780

cccgtggccg ccagaagcac cagcatcatt gccaccatcg gccctgccag cagaagcgtg      840

gagcggctga aagagatgat caaggccggc atgaatatcg cccggctgaa cttctcccac      900

ggcagccacg agtaccacgc agagagcatt gccaacgtcc gggaggccgt ggagagcttt      960

gccggcagcc ccctgagcta cagacccgtg gccattgccc tggacaccaa gggccccgag     1020

atcagaacag gaattctgca gggagggcct gagagcgagg tggagctggt gaagggcagc     1080

caagtgctgg tgaccgtgga ccccgccttc agaaccagag gcaacgccaa cacagtgtgg     1140

gtggactacc ccaacatcgt gcgggtggtg cctgtgggcg gcagaatcta catcgacgac     1200

ggcctgatca gcctggtggt gcagaagatc ggacctgagg gcctggtgac ccaggtcgag     1260

aatggcggcg tgctgggcag cagaaagggc gtgaatctgc caggcgccca ggtggacctg     1320

cctggcctgt ctgagcagga cgtgagagac ctgagatttg gcgtggagca cggcgtggac     1380

atcgtgttcg ccagcttcgt gcggaaggcc tctgatgtgg ccgccgtgag agccgctctg     1440

ggccctgaag gccacggcat caagatcatc agcaagatcg agaaccacga gggcgtgaag     1500

cggttcgacg agatcctgga agtgtccgac ggcatcatgg tggccagagg cgacctgggc     1560

atcgagatcc ccgccgagaa ggtgttcctg gcccagaaaa tgatgatcgg acggtgcaac     1620

ctggccggca aacctgtggt gtgcgccacc cagatgctgg aaagcatgat caccaagccc     1680

agacccacca gagccgagac aagcgacgtg gccaacgccg tgctggatgg cgctgactgc     1740

atcatgctgt ccggcgagac agccaagggc aacttccccg tggaggccgt gaagatgcag     1800

cacgccattg ccagagaagc cgaggccgcc gtgtaccacc ggcagctgtt cgaggaactg     1860

cggagagccg cccctctgag cagagatccc accgaagtga ccgccatcgg agccgtggaa     1920

gccgccttca agtgctgcgc cgctgcaatc atcgtgctga ccaccacagg cagaagcgcc     1980

cagctgctgt ccagatacag acccagagcc gccgtgatcg ccgtgacaag atccgcccag     2040

gccgctagac aggtccacct gtgcagaggc gtgttccccc tgctgtaccg ggagcctccc     2100

gaggccatct gggccgacga cgtggacaga cgggtgcagt tcggcatcga gagcggcaag     2160

ctgcggggct tcctgagagt gggcgacctg gtgatcgtgg tgacaggctg gcggcctggc     2220

agcggctaca ccaacatcat gagggtgctg tccatcagct gaccgcggtc tagaggatcc     2280

cccgggctgc aggaattcga gcatcttacc gccatttatt cccatatttg ttctgttttt     2340

cttgatttgg gtatacattt aaatgttaat aaaacaaaat ggtggggcaa tcatttacat     2400

ttttagggat atgtaattac tagttcaggt gtattgccac aagacaaaca tgttaagaaa     2460

ctttcccgtt atttacgctc tgttcctgtt aatcaacctc tggattacaa aatttgtgaa     2520

agattgactg atattcttaa ctatgttgct ccttttacgc tgtgtggata tgctgcttta     2580

atgcctctgt atcatgctat tgcttcccgt acggctttcg ttttctcctc cttgtataaa     2640

tcctggttgc tgtctcttta tgaggagttg tggcccgttg tccgtcaacg tggcgtggtg     2700

tgctctgtgt ttgctgacgc aacccccact ggctggggca ttgccaccac ctgtcaactc     2760

ctttctggga ctttcgcttt ccccctcccg atcgccacgg cagaactcat cgccgcctgc     2820

cttgcccgct gctggacagg ggctaggttg ctgggcactg ataattccgt ggtgttgtcg     2880

gggaagggcc                                                            2890


