                         SEQUENCE LISTING

<110>  The United States of America, as Represented by the 
       Secretary, Department of Health and Human SErvices
 
<120>  RECOMBINANT HIV-1 ENVELOPE PROTEINS AND THEIR USE

<130>  4239-99642-02

<150>  US 62/572,973
<151>  2017-10-16

<160>  38    

<170>  PatentIn version 3.5

<210>  1
<211>  856
<212>  PRT
<213>  human immunodeficiency virus

<400>  1

Met Arg Val Lys Glu Lys Tyr Gln His Leu Trp Arg Trp Gly Trp Arg 
1               5                   10                  15      


Trp Gly Thr Met Leu Leu Gly Met Leu Met Ile Cys Ser Ala Thr Glu 
            20                  25                  30          


Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala 
        35                  40                  45              


Thr Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr Glu 
    50                  55                  60                  


Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Val Val Leu Val Asn Val Thr Glu Asn Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asp Met Val Glu Gln Met His Glu Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Ser 
        115                 120                 125             


Leu Lys Cys Thr Asp Leu Lys Asn Asp Thr Asn Thr Asn Ser Ser Ser 
    130                 135                 140                 


Gly Arg Met Ile Met Glu Lys Gly Glu Ile Lys Asn Cys Ser Phe Asn 
145                 150                 155                 160 


Ile Ser Thr Ser Ile Arg Gly Lys Val Gln Lys Glu Tyr Ala Phe Phe 
                165                 170                 175     


Tyr Lys Leu Asp Ile Ile Pro Ile Asp Asn Asp Thr Thr Ser Tyr Lys 
            180                 185                 190         


Leu Thr Ser Cys Asn Thr Ser Val Ile Thr Gln Ala Cys Pro Lys Val 
        195                 200                 205             


Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe Ala 
    210                 215                 220                 


Ile Leu Lys Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Thr 
225                 230                 235                 240 


Asn Val Ser Thr Val Gln Cys Thr His Gly Ile Arg Pro Val Val Ser 
                245                 250                 255     


Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val Val Ile 
            260                 265                 270         


Arg Ser Val Asn Phe Thr Asp Asn Ala Lys Thr Ile Ile Val Gln Leu 
        275                 280                 285             


Asn Thr Ser Val Glu Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg 
    290                 295                 300                 


Lys Arg Ile Arg Ile Gln Arg Gly Pro Gly Arg Ala Phe Val Thr Ile 
305                 310                 315                 320 


Gly Lys Ile Gly Asn Met Arg Gln Ala His Cys Asn Ile Ser Arg Ala 
                325                 330                 335     


Lys Trp Asn Asn Thr Leu Lys Gln Ile Ala Ser Lys Leu Arg Glu Gln 
            340                 345                 350         


Phe Gly Asn Asn Lys Thr Ile Ile Phe Lys Gln Ser Ser Gly Gly Asp 
        355                 360                 365             


Pro Glu Ile Val Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr 
    370                 375                 380                 


Cys Asn Ser Thr Gln Leu Phe Asn Ser Thr Trp Phe Asn Ser Thr Trp 
385                 390                 395                 400 


Ser Thr Glu Gly Ser Asn Asn Thr Glu Gly Ser Asp Thr Ile Thr Leu 
                405                 410                 415     


Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Lys Val Gly Lys 
            420                 425                 430         


Ala Met Tyr Ala Pro Pro Ile Ser Gly Gln Ile Arg Cys Ser Ser Asn 
        435                 440                 445             


Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Ser Asn Asn Glu 
    450                 455                 460                 


Ser Glu Ile Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg 
465                 470                 475                 480 


Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val 
                485                 490                 495     


Ala Pro Thr Lys Ala Lys Arg Arg Val Val Gln Arg Glu Lys Arg Ala 
            500                 505                 510         


Val Gly Ile Gly Ala Leu Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser 
        515                 520                 525             


Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Gln Leu 
    530                 535                 540                 


Leu Ser Gly Ile Val Gln Gln Gln Asn Asn Leu Leu Arg Ala Ile Glu 
545                 550                 555                 560 


Ala Gln Gln His Leu Leu Gln Leu Thr Val Trp Gly Ile Lys Gln Leu 
                565                 570                 575     


Gln Ala Arg Ile Leu Ala Val Glu Arg Tyr Leu Lys Asp Gln Gln Leu 
            580                 585                 590         


Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Thr Thr Ala Val 
        595                 600                 605             


Pro Trp Asn Ala Ser Trp Ser Asn Lys Ser Leu Glu Gln Ile Trp Asn 
    610                 615                 620                 


His Thr Thr Trp Met Glu Trp Asp Arg Glu Ile Asn Asn Tyr Thr Ser 
625                 630                 635                 640 


Leu Ile His Ser Leu Ile Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn 
                645                 650                 655     


Glu Gln Glu Leu Leu Glu Leu Asp Lys Trp Ala Ser Leu Trp Asn Trp 
            660                 665                 670         


Phe Asn Ile Thr Asn Trp Leu Trp Tyr Ile Lys Leu Phe Ile Met Ile 
        675                 680                 685             


Val Gly Gly Leu Val Gly Leu Arg Ile Val Phe Ala Val Leu Ser Ile 
    690                 695                 700                 


Val Asn Arg Val Arg Gln Gly Tyr Ser Pro Leu Ser Phe Gln Thr His 
705                 710                 715                 720 


Leu Pro Thr Pro Arg Gly Pro Asp Arg Pro Glu Gly Ile Glu Glu Glu 
                725                 730                 735     


Gly Gly Glu Arg Asp Arg Asp Arg Ser Ile Arg Leu Val Asn Gly Ser 
            740                 745                 750         


Leu Ala Leu Ile Trp Asp Asp Leu Arg Ser Leu Cys Leu Phe Ser Tyr 
        755                 760                 765             


His Arg Leu Arg Asp Leu Leu Leu Ile Val Thr Arg Ile Val Glu Leu 
    770                 775                 780                 


Leu Gly Arg Arg Gly Trp Glu Ala Leu Lys Tyr Trp Trp Asn Leu Leu 
785                 790                 795                 800 


Gln Tyr Trp Ser Gln Glu Leu Lys Asn Ser Ala Val Ser Leu Leu Asn 
                805                 810                 815     


Ala Thr Ala Ile Ala Val Ala Glu Gly Thr Asp Arg Val Ile Glu Val 
            820                 825                 830         


Val Gln Gly Ala Cys Arg Ala Ile Arg His Ile Pro Arg Arg Ile Arg 
        835                 840                 845             


Gln Gly Leu Glu Arg Ile Leu Leu 
    850                 855     


<210>  2
<211>  634
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  2

Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Thr Glu Lys His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Ile His Leu Glu Asn Val Thr Glu Glu Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Gln Cys Thr Asn Val Thr Asn Asn Ile Thr Asp Asp Met 
            100                 105                 110         


Arg Gly Glu Leu Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Val Tyr Ser Leu Phe Tyr Arg Leu Asp Val Val 
    130                 135                 140                 


Gln Ile Asn Glu Asn Gln Gly Asn Arg Ser Asn Asn Ser Asn Lys Glu 
145                 150                 155                 160 


Tyr Arg Leu Ile Asn Cys Asn Thr Ser Ala Cys Thr Gln Ala Cys Pro 
                165                 170                 175     


Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly 
            180                 185                 190         


Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro 
        195                 200                 205             


Cys Pro Ser Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val 
    210                 215                 220                 


Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val 
225                 230                 235                 240 


Met Ile Arg Ser Glu Asn Ile Thr Asn Asn Ala Lys Asn Ile Leu Val 
                245                 250                 255     


Gln Phe Asn Thr Pro Val Gln Ile Asn Cys Thr Arg Pro Asn Asn Asn 
            260                 265                 270         


Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Ala Phe Tyr Ala Thr 
        275                 280                 285             


Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Val Ser Lys 
    290                 295                 300                 


Ala Thr Trp Asn Glu Thr Leu Gly Lys Val Val Lys Gln Leu Arg Lys 
305                 310                 315                 320 


His Phe Gly Asn Asn Thr Ile Ile Arg Phe Ala Asn Ser Ser Gly Gly 
                325                 330                 335     


Asp Leu Glu Val Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe 
            340                 345                 350         


Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Thr Trp Ile Ser Asn Thr 
        355                 360                 365             


Ser Val Gln Gly Ser Asn Ser Thr Gly Ser Asn Asp Ser Ile Thr Leu 
    370                 375                 380                 


Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Ile Gly Gln 
385                 390                 395                 400 


Cys Met Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Val Ser Asn 
                405                 410                 415     


Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Ser Thr Asn Ser Thr 
            420                 425                 430         


Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg 
        435                 440                 445             


Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val 
    450                 455                 460                 


Ala Pro Thr Arg Cys Lys Arg Arg Val Val Gly Arg Arg Arg Arg Arg 
465                 470                 475                 480 


Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala 
                485                 490                 495     


Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg 
            500                 505                 510         


Asn Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala 
        515                 520                 525             


Pro Glu Ala Gln Gln His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys 
    530                 535                 540                 


Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln 
545                 550                 555                 560 


Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr 
                565                 570                 575     


Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Arg Asn Leu Ser Glu Ile 
            580                 585                 590         


Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr 
        595                 600                 605             


Thr Gln Ile Ile Tyr Gly Leu Leu Glu Glu Ser Gln Asn Gln Gln Glu 
    610                 615                 620                 


Lys Asn Glu Gln Asp Leu Leu Ala Leu Asp 
625                 630                 


<210>  3
<211>  634
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  3

Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Thr Glu Lys His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Ile His Leu Glu Asn Val Thr Glu Glu Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Gln Cys Thr Asn Val Thr Asn Asn Ile Thr Asp Asp Met 
            100                 105                 110         


Arg Gly Glu Leu Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Val Tyr Ser Leu Phe Tyr Arg Leu Asp Val Val 
    130                 135                 140                 


Gln Ile Asn Glu Asn Gln Gly Asn Arg Ser Asn Asn Ser Asn Lys Glu 
145                 150                 155                 160 


Tyr Arg Leu Ile Asn Cys Asn Thr Ser Ala Cys Thr Gln Ala Cys Pro 
                165                 170                 175     


Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly 
            180                 185                 190         


Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro 
        195                 200                 205             


Cys Pro Ser Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val 
    210                 215                 220                 


Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val 
225                 230                 235                 240 


Met Ile Arg Ser Glu Asn Ile Thr Asn Asn Ala Lys Asn Ile Leu Val 
                245                 250                 255     


Gln Phe Asn Thr Pro Val Gln Ile Asn Cys Thr Arg Pro Asn Asn Met 
            260                 265                 270         


Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Ala Phe Tyr Ala Leu 
        275                 280                 285             


Gly Asp Ile Ile Gly Asp Ile Arg Gln Pro His Cys Asn Val Ser Lys 
    290                 295                 300                 


Ala Thr Trp Asn Glu Thr Leu Gly Lys Val Val Lys Gln Leu Arg Lys 
305                 310                 315                 320 


His Phe Gly Asn Asn Thr Ile Ile Arg Phe Ala Asn Ser Ser Gly Gly 
                325                 330                 335     


Asp Leu Glu Val Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe 
            340                 345                 350         


Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Thr Trp Ile Ser Asn Thr 
        355                 360                 365             


Ser Val Gln Gly Ser Asn Ser Thr Gly Ser Asn Asp Ser Ile Thr Leu 
    370                 375                 380                 


Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Ile Gly Gln 
385                 390                 395                 400 


Cys Met Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Val Ser Asn 
                405                 410                 415     


Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Ser Thr Asn Ser Thr 
            420                 425                 430         


Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg 
        435                 440                 445             


Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val 
    450                 455                 460                 


Ala Pro Thr Arg Cys Lys Arg Arg Val Val Gly Arg Arg Arg Arg Arg 
465                 470                 475                 480 


Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala 
                485                 490                 495     


Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg 
            500                 505                 510         


Asn Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala 
        515                 520                 525             


Pro Glu Ala Gln Gln His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys 
    530                 535                 540                 


Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln 
545                 550                 555                 560 


Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr 
                565                 570                 575     


Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Arg Asn Leu Ser Glu Ile 
            580                 585                 590         


Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr 
        595                 600                 605             


Thr Gln Ile Ile Tyr Gly Leu Leu Glu Glu Ser Gln Asn Gln Gln Glu 
    610                 615                 620                 


Lys Asn Glu Gln Asp Leu Leu Ala Leu Asp 
625                 630                 


<210>  4
<211>  662
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  4

Ser Arg Ala Thr Met Pro Met Gly Ser Leu Gln Pro Leu Ala Thr Leu 
1               5                   10                  15      


Tyr Leu Leu Gly Met Leu Val Ala Ser Val Leu Ala Ala Glu Asn Leu 
            20                  25                  30          


Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Asp Ala Glu Thr 
        35                  40                  45              


Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu Thr Glu Lys His 
    50                  55                  60                  


Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln 
65                  70                  75                  80  


Glu Ile His Leu Glu Asn Val Thr Glu Glu Phe Asn Met Trp Lys Asn 
                85                  90                  95      


Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser Leu Trp Asp Gln 
            100                 105                 110         


Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Gln 
        115                 120                 125             


Cys Thr Asn Val Thr Asn Asn Ile Thr Asp Asp Met Arg Gly Glu Leu 
    130                 135                 140                 


Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg Asp Lys Lys Gln 
145                 150                 155                 160 


Lys Val Tyr Ser Leu Phe Tyr Arg Leu Asp Val Val Gln Ile Asn Glu 
                165                 170                 175     


Asn Gln Gly Asn Arg Ser Asn Asn Ser Asn Lys Glu Tyr Arg Leu Ile 
            180                 185                 190         


Asn Cys Asn Thr Ser Ala Cys Thr Gln Ala Cys Pro Lys Val Ser Phe 
        195                 200                 205             


Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe Ala Ile Leu 
    210                 215                 220                 


Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro Cys Pro Ser Val 
225                 230                 235                 240 


Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln 
                245                 250                 255     


Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val Met Ile Arg Ser 
            260                 265                 270         


Glu Asn Ile Thr Asn Asn Ala Lys Asn Ile Leu Val Gln Phe Asn Thr 
        275                 280                 285             


Pro Val Gln Ile Asn Cys Thr Arg Pro Asn Asn Met Thr Arg Lys Ser 
    290                 295                 300                 


Ile Arg Ile Gly Pro Gly Gln Ala Phe Tyr Ala Leu Gly Asp Ile Ile 
305                 310                 315                 320 


Gly Asp Ile Arg Gln Pro His Cys Asn Val Ser Lys Ala Thr Trp Asn 
                325                 330                 335     


Glu Thr Leu Gly Lys Val Val Lys Gln Leu Arg Lys His Phe Gly Asn 
            340                 345                 350         


Asn Thr Ile Ile Arg Phe Ala Asn Ser Ser Gly Gly Asp Leu Glu Val 
        355                 360                 365             


Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr 
    370                 375                 380                 


Ser Gly Leu Phe Asn Ser Thr Trp Ile Ser Asn Thr Ser Val Gln Gly 
385                 390                 395                 400 


Ser Asn Ser Thr Gly Ser Asn Asp Ser Ile Thr Leu Pro Cys Arg Ile 
                405                 410                 415     


Lys Gln Ile Ile Asn Met Trp Gln Arg Ile Gly Gln Cys Met Tyr Ala 
            420                 425                 430         


Pro Pro Ile Gln Gly Val Ile Arg Cys Val Ser Asn Ile Thr Gly Leu 
        435                 440                 445             


Ile Leu Thr Arg Asp Gly Gly Ser Thr Asn Ser Thr Thr Glu Thr Phe 
    450                 455                 460                 


Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr 
465                 470                 475                 480 


Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro Thr Arg 
                485                 490                 495     


Cys Lys Arg Arg Val Val Gly Arg Arg Arg Arg Arg Arg Ala Val Gly 
            500                 505                 510         


Ile Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met 
        515                 520                 525             


Gly Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Asn Leu Leu Ser 
    530                 535                 540                 


Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln 
545                 550                 555                 560 


Gln His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala 
                565                 570                 575     


Arg Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly 
            580                 585                 590         


Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Asn Val Pro Trp 
        595                 600                 605             


Asn Ser Ser Trp Ser Asn Arg Asn Leu Ser Glu Ile Trp Asp Asn Met 
    610                 615                 620                 


Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr Gln Ile Ile 
625                 630                 635                 640 


Tyr Gly Leu Leu Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln 
                645                 650                 655     


Asp Leu Leu Ala Leu Asp 
            660         


<210>  5
<211>  623
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  5

Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Glu Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Lys Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Met Val Leu Lys Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Val Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Asn Cys Thr Asn Ala Thr Ala Ser Asn Ser Ser Ile Ile 
            100                 105                 110         


Glu Gly Met Lys Asn Cys Ser Phe Asn Ile Thr Thr Glu Leu Arg Asp 
        115                 120                 125             


Lys Arg Glu Lys Lys Asn Ala Leu Phe Tyr Lys Leu Asp Ile Val Gln 
    130                 135                 140                 


Leu Asp Gly Asn Ser Ser Gln Tyr Arg Leu Ile Asn Cys Asn Thr Ser 
145                 150                 155                 160 


Val Cys Thr Gln Ala Cys Pro Lys Val Ser Phe Asp Pro Ile Pro Ile 
                165                 170                 175     


His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn Lys 
            180                 185                 190         


Thr Phe Thr Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val Gln Cys 
        195                 200                 205             


Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly 
    210                 215                 220                 


Ser Leu Ala Glu Gly Glu Ile Ile Ile Arg Ser Glu Asn Ile Thr Asn 
225                 230                 235                 240 


Asn Val Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Lys Ile Glu 
                245                 250                 255     


Cys Thr Arg Pro Asn Asn Lys Thr Arg Thr Ser Ile Arg Ile Gly Pro 
            260                 265                 270         


Gly Gln Ala Phe Tyr Ala Thr Gly Gln Val Ile Gly Asp Ile Arg Glu 
        275                 280                 285             


Ala Tyr Cys Asn Ile Asn Glu Ser Lys Trp Asn Glu Thr Leu Gln Arg 
    290                 295                 300                 


Val Ser Lys Lys Leu Lys Glu Tyr Phe Pro His Lys Asn Ile Thr Phe 
305                 310                 315                 320 


Gln Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn 
                325                 330                 335     


Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Ser Leu Phe Asn Arg 
            340                 345                 350         


Thr Tyr Met Ala Asn Ser Thr Asp Met Ala Asn Ser Thr Glu Thr Asn 
        355                 360                 365             


Ser Thr Arg Thr Ile Thr Ile His Cys Arg Ile Lys Gln Ile Ile Asn 
    370                 375                 380                 


Met Trp Gln Glu Val Gly Arg Cys Met Tyr Ala Pro Pro Ile Ala Gly 
385                 390                 395                 400 


Asn Ile Thr Cys Ile Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp 
                405                 410                 415     


Gly Gly Lys Asn Asn Thr Glu Thr Phe Arg Pro Gly Gly Gly Asn Met 
            420                 425                 430         


Lys Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile 
        435                 440                 445             


Glu Pro Leu Gly Val Ala Pro Thr Arg Cys Lys Arg Arg Val Val Gly 
    450                 455                 460                 


Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly 
465                 470                 475                 480 


Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu 
                485                 490                 495     


Thr Val Gln Ala Arg Asn Leu Leu Ser Gly Ile Val Gln Gln Gln Ser 
            500                 505                 510         


Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Leu Leu Lys Leu Thr 
        515                 520                 525             


Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg 
    530                 535                 540                 


Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys 
545                 550                 555                 560 


Leu Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Arg 
                565                 570                 575     


Asn Leu Ser Glu Ile Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys 
            580                 585                 590         


Glu Ile Ser Asn Tyr Thr Gln Ile Ile Tyr Gly Leu Leu Glu Glu Ser 
        595                 600                 605             


Gln Asn Gln Gln Glu Lys Asn Glu Gln Asp Leu Leu Ala Leu Asp 
    610                 615                 620             


<210>  6
<211>  623
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  6

Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Glu Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Lys Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Met Val Leu Lys Asn Val Thr Glu Asn Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Val Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Asn Cys Thr Asn Ala Thr Ala Ser Asn Ser Ser Ile Ile 
            100                 105                 110         


Glu Gly Met Lys Asn Cys Ser Phe Asn Ile Thr Thr Glu Leu Arg Asp 
        115                 120                 125             


Lys Arg Glu Lys Lys Asn Ala Leu Phe Tyr Lys Leu Asp Ile Val Gln 
    130                 135                 140                 


Leu Asp Gly Asn Ser Ser Gln Tyr Arg Leu Ile Asn Cys Asn Thr Ser 
145                 150                 155                 160 


Val Cys Thr Gln Ala Cys Pro Lys Val Ser Phe Asp Pro Ile Pro Ile 
                165                 170                 175     


His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn Lys 
            180                 185                 190         


Thr Phe Thr Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val Gln Cys 
        195                 200                 205             


Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly 
    210                 215                 220                 


Ser Leu Ala Glu Gly Glu Ile Ile Ile Arg Ser Glu Asn Ile Thr Asn 
225                 230                 235                 240 


Asn Val Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Lys Ile Glu 
                245                 250                 255     


Cys Thr Arg Pro Asn Asn Met Thr Arg Thr Ser Ile Arg Ile Gly Pro 
            260                 265                 270         


Gly Gln Ala Phe Tyr Ala Leu Gly Gln Val Ile Gly Asp Ile Arg Glu 
        275                 280                 285             


Pro Tyr Cys Asn Ile Asn Glu Ser Lys Trp Asn Glu Thr Leu Gln Arg 
    290                 295                 300                 


Val Ser Lys Lys Leu Lys Glu Tyr Phe Pro His Lys Asn Ile Thr Phe 
305                 310                 315                 320 


Gln Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn 
                325                 330                 335     


Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Ser Leu Phe Asn Arg 
            340                 345                 350         


Thr Tyr Met Ala Asn Ser Thr Asp Met Ala Asn Ser Thr Glu Thr Asn 
        355                 360                 365             


Ser Thr Arg Thr Ile Thr Ile His Cys Arg Ile Lys Gln Ile Ile Asn 
    370                 375                 380                 


Met Trp Gln Glu Val Gly Arg Cys Met Tyr Ala Pro Pro Ile Ala Gly 
385                 390                 395                 400 


Asn Ile Thr Cys Ile Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp 
                405                 410                 415     


Gly Gly Lys Asn Asn Thr Glu Thr Phe Arg Pro Gly Gly Gly Asn Met 
            420                 425                 430         


Lys Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile 
        435                 440                 445             


Glu Pro Leu Gly Val Ala Pro Thr Arg Cys Lys Arg Arg Val Val Gly 
    450                 455                 460                 


Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly 
465                 470                 475                 480 


Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu 
                485                 490                 495     


Thr Val Gln Ala Arg Asn Leu Leu Ser Gly Ile Val Gln Gln Gln Ser 
            500                 505                 510         


Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Leu Leu Lys Leu Thr 
        515                 520                 525             


Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg 
    530                 535                 540                 


Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys 
545                 550                 555                 560 


Leu Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Arg 
                565                 570                 575     


Asn Leu Ser Glu Ile Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys 
            580                 585                 590         


Glu Ile Ser Asn Tyr Thr Gln Ile Ile Tyr Gly Leu Leu Glu Glu Ser 
        595                 600                 605             


Gln Asn Gln Gln Glu Lys Asn Glu Gln Asp Leu Leu Ala Leu Asp 
    610                 615                 620             


<210>  7
<211>  4
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Furin cleavage site

<400>  7

Arg Glu Lys Arg 
1               


<210>  8
<211>  6
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Furin cleavage site

<400>  8

Arg Arg Arg Arg Arg Arg 
1               5       


<210>  9
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Peptide linker

<400>  9

Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
1               5                   10  


<210>  10
<211>  27
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Transmembrane domain

<400>  10

Lys Ile Phe Ile Met Ile Val Gly Gly Leu Ile Gly Leu Arg Ile Val 
1               5                   10                  15      


Phe Ala Val Leu Ser Val Ile His Arg Val Arg 
            20                  25          


<210>  11
<211>  23
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Transmembrane domain

<400>  11

Ile Leu Ala Ile Tyr Ser Thr Val Ala Ser Ser Leu Val Leu Leu Val 
1               5                   10                  15      


Ser Leu Gly Ala Ile Ser Phe 
            20              


<210>  12
<211>  29
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Transmembrane domain

<400>  12

Ile Ile Thr Ile Gly Ser Ile Cys Met Val Val Gly Ile Ile Ser Leu 
1               5                   10                  15      


Ile Leu Gln Ile Gly Asn Ile Ile Ser Ile Trp Val Ser 
            20                  25                  


<210>  13
<211>  26
<212>  PRT
<213>  Artificial sequence

<220>
<223>  T4 fibritin domain

<400>  13

Gly Tyr Ile Pro Glu Ala Pro Arg Asp Gly Gln Ala Tyr Val Arg Lys 
1               5                   10                  15      


Asp Gly Glu Trp Val Leu Leu Ser Thr Phe 
            20                  25      


<210>  14
<211>  154
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant protein nanoparticle subunit

<400>  14

Met Gln Ile Tyr Glu Gly Lys Leu Thr Ala Glu Gly Leu Arg Phe Gly 
1               5                   10                  15      


Ile Val Ala Ser Arg Phe Asn His Ala Leu Val Asp Arg Leu Val Glu 
            20                  25                  30          


Gly Ala Ile Asp Ala Ile Val Arg His Gly Gly Arg Glu Glu Asp Ile 
        35                  40                  45              


Thr Leu Val Arg Val Pro Gly Ser Trp Glu Ile Pro Val Ala Ala Gly 
    50                  55                  60                  


Glu Leu Ala Arg Lys Glu Asp Ile Asp Ala Val Ile Ala Ile Gly Val 
65                  70                  75                  80  


Leu Ile Arg Gly Ala Thr Pro His Phe Asp Tyr Ile Ala Ser Glu Val 
                85                  90                  95      


Ser Lys Gly Leu Ala Asp Leu Ser Leu Glu Leu Arg Lys Pro Ile Thr 
            100                 105                 110         


Phe Gly Val Ile Thr Ala Asp Thr Leu Glu Gln Ala Ile Glu Arg Ala 
        115                 120                 125             


Gly Thr Lys His Gly Asn Lys Gly Trp Glu Ala Ala Leu Ser Ala Ile 
    130                 135                 140                 


Glu Met Ala Asn Leu Phe Lys Ser Leu Arg 
145                 150                 


<210>  15
<211>  173
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant protein nanoparticle subunit

<400>  15

Glu Ser Gln Val Arg Gln Gln Phe Ser Lys Asp Ile Glu Lys Leu Leu 
1               5                   10                  15      


Asn Glu Gln Val Asn Lys Glu Met Gln Ser Ser Asn Leu Tyr Met Ser 
            20                  25                  30          


Met Ser Ser Trp Cys Tyr Thr His Ser Leu Asp Gly Ala Gly Leu Phe 
        35                  40                  45              


Leu Phe Asp His Ala Ala Glu Glu Tyr Glu His Ala Lys Lys Leu Ile 
    50                  55                  60                  


Ile Phe Leu Asn Glu Asn Asn Val Pro Val Gln Leu Thr Ser Ile Ser 
65                  70                  75                  80  


Ala Pro Glu His Lys Phe Glu Gly Leu Thr Gln Ile Phe Gln Lys Ala 
                85                  90                  95      


Tyr Glu His Glu Gln His Ile Ser Glu Ser Ile Asn Asn Ile Val Asp 
            100                 105                 110         


His Ala Ile Lys Ser Lys Asp His Ala Thr Phe Asn Phe Leu Gln Trp 
        115                 120                 125             


Tyr Val Ala Glu Gln His Glu Glu Glu Val Leu Phe Lys Asp Ile Leu 
    130                 135                 140                 


Asp Lys Ile Glu Leu Ile Gly Asn Glu Asn His Gly Leu Tyr Leu Ala 
145                 150                 155                 160 


Asp Gln Tyr Val Lys Gly Ile Ala Lys Ser Arg Lys Ser 
                165                 170             


<210>  16
<211>  265
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant protein nanoparticle subunit

<400>  16

Met Glu Phe Leu Lys Arg Ser Phe Ala Pro Leu Thr Glu Lys Gln Trp 
1               5                   10                  15      


Gln Glu Ile Asp Asn Arg Ala Arg Glu Ile Phe Lys Thr Gln Leu Tyr 
            20                  25                  30          


Gly Arg Lys Phe Val Asp Val Glu Gly Pro Tyr Gly Trp Glu Tyr Ala 
        35                  40                  45              


Ala His Pro Leu Gly Glu Val Glu Val Leu Ser Asp Glu Asn Glu Val 
    50                  55                  60                  


Val Lys Trp Gly Leu Arg Lys Ser Leu Pro Leu Ile Glu Leu Arg Ala 
65                  70                  75                  80  


Thr Phe Thr Leu Asp Leu Trp Glu Leu Asp Asn Leu Glu Arg Gly Lys 
                85                  90                  95      


Pro Asn Val Asp Leu Ser Ser Leu Glu Glu Thr Val Arg Lys Val Ala 
            100                 105                 110         


Glu Phe Glu Asp Glu Val Ile Phe Arg Gly Cys Glu Lys Ser Gly Val 
        115                 120                 125             


Lys Gly Leu Leu Ser Phe Glu Glu Arg Lys Ile Glu Cys Gly Ser Thr 
    130                 135                 140                 


Pro Lys Asp Leu Leu Glu Ala Ile Val Arg Ala Leu Ser Ile Phe Ser 
145                 150                 155                 160 


Lys Asp Gly Ile Glu Gly Pro Tyr Thr Leu Val Ile Asn Thr Asp Arg 
                165                 170                 175     


Trp Ile Asn Phe Leu Lys Glu Glu Ala Gly His Tyr Pro Leu Glu Lys 
            180                 185                 190         


Arg Val Glu Glu Cys Leu Arg Gly Gly Lys Ile Ile Thr Thr Pro Arg 
        195                 200                 205             


Ile Glu Asp Ala Leu Val Val Ser Glu Arg Gly Gly Asp Phe Lys Leu 
    210                 215                 220                 


Ile Leu Gly Gln Asp Leu Ser Ile Gly Tyr Glu Asp Arg Glu Lys Asp 
225                 230                 235                 240 


Ala Val Arg Leu Phe Ile Thr Glu Thr Phe Thr Phe Gln Val Val Asn 
                245                 250                 255     


Pro Glu Ala Leu Ile Leu Leu Lys Phe 
            260                 265 


<210>  17
<211>  1904
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  17
gccgaaaacc tgtgggtcac cgtgtattat ggagtgcccg tctggaaaga tgctgaaact       60

accctgttct gtgcctctga tgctaaggcc tacgagaccg aaaagcacaa tgtctgggct      120

actcatgcat gcgtgcccac cgacccaaac ccccaggaga tccacctgga aaatgtgacc      180

gaggaattca acatgtggaa aaacaatatg gtggagcaga tgcatacaga catcattagc      240

ctgtgggatc agtccctgaa gccctgcgtc aaactgactc ctctgtgcgt gaccctgcag      300

tgtaccaatg tcacaaacaa tatcaccgac gatatgaggg gcgagctgaa gaattgtagc      360

ttcaacatga ccacagaact gagagacaag aaacagaaag tgtactccct gttttatagg      420

ctggatgtgg tccagatcaa tgagaaccag gggaatcgga gcaacaattc caacaaggaa      480

tacagactga tcaattgcaa cacttccgcc tgtacccagg cttgtcctaa agtgtctttt      540

gagcctatcc caattcatta ttgcgcccca gctggcttcg ccatcctgaa gtgtaaagat      600

aagaagttca acggaactgg cccctgccct tccgtgtcta cagtccagtg tactcacggg      660

attaagcctg tggtctctac acagctgctg ctgaatggaa gtctggctga ggaagaagtg      720

atgatccgga gcgagaacat taccaacaat gccaagaata tcctggtcca gttcaacaca      780

ccagtgcaga ttaattgcac aagacccaac aatatgactc gaaaatctat ccggattggg      840

ccaggacagg ccttttacgc tctgggggac atcattggag atatcagaca gcctcactgt      900

aatgtgagta aggcaacctg gaacgagaca ctgggcaagg tggtcaaaca gctgaggaaa      960

catttcggga ataacaccat cattcgcttt gccaatagct ccggagggga cctggaggtc     1020

actacccact ccttcaactg cggaggcgaa ttcttttact gtaacacatc tggcctgttt     1080

aatagtacat ggatctctaa cactagtgtg cagggcagta attcaactgg gtcaaacgat     1140

agcatcaccc tgccatgccg aattaagcag atcattaata tgtggcagcg gatcggccag     1200

tgcatgtatg ccccccctat ccagggggtc attcgctgcg tgagcaatat caccggactg     1260

attctgacac gagacggggg cagcaccaac tctacaactg aaacattccg gcccggcggg     1320

ggagacatga gagataactg gaggtccgag ctgtacaagt ataaagtggt caagatcgaa     1380

cctctgggag tggcaccaac cagatgcaag cgaagagtgg tcggacgaag gaggaggagg     1440

cgagcagtcg gaattggggc cgtgttcctg ggatttctgg gcgccgctgg gagtacaatg     1500

ggagcagcct caatgactct gaccgtgcag gccaggaatc tgctgagcgg catcgtccag     1560

cagcagtcca acctgctgcg cgctcctgaa gcacagcagc acctgctgaa gctgaccgtg     1620

tggggcatca aacagctgca ggctagggtg ctggcagtcg agcggtacct gagagaccag     1680

cagctgctgg gaatctgggg ctgctctggg aagctgattt gttgcacaaa tgtgccttgg     1740

aactctagtt ggtcaaatcg caacctgagc gagatctggg acaatatgac ttggctgcag     1800

tgggataaag aaattagtaa ctacacccag atcatctacg gcctgctgga agagtcacag     1860

aatcagcagg agaagaacga acaggacctg ctggctctgg attg                      1904


<210>  18
<211>  1988
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  18
tctagagcca ccatgcctat ggggagcctg cagcctctgg caaccctgta tctgctggga       60

atgctggtcg caagtgtcct ggccgccgaa aacctgtggg tcaccgtgta ttatggagtg      120

cccgtctgga aagatgctga aactaccctg ttctgtgcct ctgatgctaa ggcctacgag      180

accgaaaagc acaatgtctg ggctactcat gcatgcgtgc ccaccgaccc aaacccccag      240

gagatccacc tggaaaatgt gaccgaggaa ttcaacatgt ggaaaaacaa tatggtggag      300

cagatgcata cagacatcat tagcctgtgg gatcagtccc tgaagccctg cgtcaaactg      360

actcctctgt gcgtgaccct gcagtgtacc aatgtcacaa acaatatcac cgacgatatg      420

aggggcgagc tgaagaattg tagcttcaac atgaccacag aactgagaga caagaaacag      480

aaagtgtact ccctgtttta taggctggat gtggtccaga tcaatgagaa ccaggggaat      540

cggagcaaca attccaacaa ggaatacaga ctgatcaatt gcaacacttc cgcctgtacc      600

caggcttgtc ctaaagtgtc ttttgagcct atcccaattc attattgcgc cccagctggc      660

ttcgccatcc tgaagtgtaa agataagaag ttcaacggaa ctggcccctg cccttccgtg      720

tctacagtcc agtgtactca cgggattaag cctgtggtct ctacacagct gctgctgaat      780

ggaagtctgg ctgaggaaga agtgatgatc cggagcgaga acattaccaa caatgccaag      840

aatatcctgg tccagttcaa cacaccagtg cagattaatt gcacaagacc caacaatatg      900

actcgaaaat ctatccggat tgggccagga caggcctttt acgctctggg ggacatcatt      960

ggagatatca gacagcctca ctgtaatgtg agtaaggcaa cctggaacga gacactgggc     1020

aaggtggtca aacagctgag gaaacatttc gggaataaca ccatcattcg ctttgccaat     1080

agctccggag gggacctgga ggtcactacc cactccttca actgcggagg cgaattcttt     1140

tactgtaaca catctggcct gtttaatagt acatggatct ctaacactag tgtgcagggc     1200

agtaattcaa ctgggtcaaa cgatagcatc accctgccat gccgaattaa gcagatcatt     1260

aatatgtggc agcggatcgg ccagtgcatg tatgcccccc ctatccaggg ggtcattcgc     1320

tgcgtgagca atatcaccgg actgattctg acacgagacg ggggcagcac caactctaca     1380

actgaaacat tccggcccgg cgggggagac atgagagata actggaggtc cgagctgtac     1440

aagtataaag tggtcaagat cgaacctctg ggagtggcac caaccagatg caagcgaaga     1500

gtggtcggac gaaggaggag gaggcgagca gtcggaattg gggccgtgtt cctgggattt     1560

ctgggcgccg ctgggagtac aatgggagca gcctcaatga ctctgaccgt gcaggccagg     1620

aatctgctga gcggcatcgt ccagcagcag tccaacctgc tgcgcgctcc tgaagcacag     1680

cagcacctgc tgaagctgac cgtgtggggc atcaaacagc tgcaggctag ggtgctggca     1740

gtcgagcggt acctgagaga ccagcagctg ctgggaatct ggggctgctc tgggaagctg     1800

atttgttgca caaatgtgcc ttggaactct agttggtcaa atcgcaacct gagcgagatc     1860

tgggacaata tgacttggct gcagtgggat aaagaaatta gtaactacac ccagatcatc     1920

tacggcctgc tggaagagtc acagaatcag caggagaaga acgaacagga cctgctggct     1980

ctggattg                                                              1988


<210>  19
<211>  625
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  19

Ala Gly Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Arg Glu 
1               5                   10                  15      


Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ser Tyr Glu Lys 
            20                  25                  30          


Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro 
        35                  40                  45              


Asn Pro Gln Glu Leu Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met 
    50                  55                  60                  


Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu 
65                  70                  75                  80  


Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val 
                85                  90                  95      


Thr Leu Asn Cys Ser Asp Ala Lys Val Asn Ala Thr Tyr Lys Gly Thr 
            100                 105                 110         


Arg Glu Glu Ile Lys Asn Cys Ser Phe Asn Ala Thr Thr Glu Leu Arg 
        115                 120                 125             


Asp Lys Lys Arg Arg Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val 
    130                 135                 140                 


Pro Leu Ser Gly Glu Gly Asn Asn Asn Ser Glu Tyr Arg Leu Ile Asn 
145                 150                 155                 160 


Cys Asn Thr Ser Val Ile Thr Gln Ile Cys Pro Lys Val Thr Phe Asp 
                165                 170                 175     


Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys 
            180                 185                 190         


Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser 
        195                 200                 205             


Thr Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu 
    210                 215                 220                 


Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu 
225                 230                 235                 240 


Asn Leu Thr Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Glu Ser 
                245                 250                 255     


Val Glu Ile Thr Cys Thr Arg Pro Asn Asn Met Thr Arg Lys Ser Val 
            260                 265                 270         


Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Leu Gly Asp Ile Ile Gly 
        275                 280                 285             


Asp Ile Arg Gln Pro His Cys Asn Ile Ser Glu Ile Lys Trp Glu Lys 
    290                 295                 300                 


Thr Leu Gln Arg Val Ser Glu Lys Leu Arg Glu His Phe Asn Lys Thr 
305                 310                 315                 320 


Ile Ile Phe Asn Gln Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His 
                325                 330                 335     


Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Asp Leu 
            340                 345                 350         


Phe Phe Asn Lys Thr Phe Asn Glu Thr Tyr Ser Thr Gly Ser Asn Ser 
        355                 360                 365             


Thr Asn Ser Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn 
    370                 375                 380                 


Met Trp Gln Glu Val Gly Arg Ala Met Tyr Ala Pro Pro Ile Ala Gly 
385                 390                 395                 400 


Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp 
                405                 410                 415     


Gly Gly Gly Asn Asn Ser Thr Lys Glu Thr Phe Arg Pro Gly Gly Gly 
            420                 425                 430         


Asn Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val 
        435                 440                 445             


Glu Val Lys Pro Leu Gly Ile Ala Pro Thr Glu Cys Asn Arg Thr Val 
    450                 455                 460                 


Val Gln Arg Arg Arg Arg Arg Arg Ala Val Gly Leu Gly Ala Val Phe 
465                 470                 475                 480 


Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Asn 
                485                 490                 495     


Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
            500                 505                 510         


Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Met Leu Gln 
        515                 520                 525             


Leu Gly Val Trp Gly Phe Lys Gln Leu Gln Ala Arg Val Leu Ala Ile 
    530                 535                 540                 


Glu Arg Tyr Leu Glu Val Gln Gln Leu Leu Gly Met Trp Gly Cys Ser 
545                 550                 555                 560 


Gly Lys Leu Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser Trp Ser 
                565                 570                 575     


Asn Lys Thr Tyr Asn Glu Ile Trp Asp Asn Met Thr Trp Met Gln Trp 
            580                 585                 590         


Asp Arg Glu Ile Gly Asn Tyr Thr Asp Thr Ile Tyr Lys Leu Leu Glu 
        595                 600                 605             


Val Ser Gln Phe Gln Gln Glu Ile Asn Glu Lys Asp Asn Leu Thr Leu 
    610                 615                 620                 


Asp 
625 


<210>  20
<211>  654
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  20

Met Asp Ser Lys Gly Ser Ser Gln Lys Gly Ser Arg Leu Leu Leu Leu 
1               5                   10                  15      


Leu Val Val Ser Asn Leu Leu Leu Pro Gln Gly Val Leu Ala Gly Leu 
            20                  25                  30          


Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Arg Glu Ala Lys Thr 
        35                  40                  45              


Thr Leu Phe Cys Ala Ser Asp Ala Lys Ser Tyr Glu Lys Glu Val His 
    50                  55                  60                  


Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln 
65                  70                  75                  80  


Glu Leu Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn 
                85                  90                  95      


Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp Gln 
            100                 105                 110         


Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn 
        115                 120                 125             


Cys Ser Asp Ala Lys Val Asn Ala Thr Tyr Lys Gly Thr Arg Glu Glu 
    130                 135                 140                 


Ile Lys Asn Cys Ser Phe Asn Ala Thr Thr Glu Leu Arg Asp Lys Lys 
145                 150                 155                 160 


Arg Arg Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu Ser 
                165                 170                 175     


Gly Glu Gly Asn Asn Asn Ser Glu Tyr Arg Leu Ile Asn Cys Asn Thr 
            180                 185                 190         


Ser Val Ile Thr Gln Ile Cys Pro Lys Val Thr Phe Asp Pro Ile Pro 
        195                 200                 205             


Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
    210                 215                 220                 


Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val Gln 
225                 230                 235                 240 


Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
                245                 250                 255     


Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr 
            260                 265                 270         


Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu Ile 
        275                 280                 285             


Thr Cys Thr Arg Pro Asn Asn Met Thr Arg Lys Ser Val Arg Ile Gly 
    290                 295                 300                 


Pro Gly Gln Thr Phe Tyr Ala Leu Gly Asp Ile Ile Gly Asp Ile Arg 
305                 310                 315                 320 


Gln Pro His Cys Asn Ile Ser Glu Ile Lys Trp Glu Lys Thr Leu Gln 
                325                 330                 335     


Arg Val Ser Glu Lys Leu Arg Glu His Phe Asn Lys Thr Ile Ile Phe 
            340                 345                 350         


Asn Gln Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn 
        355                 360                 365             


Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Asp Leu Phe Phe Asn 
    370                 375                 380                 


Lys Thr Phe Asn Glu Thr Tyr Ser Thr Gly Ser Asn Ser Thr Asn Ser 
385                 390                 395                 400 


Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln 
                405                 410                 415     


Glu Val Gly Arg Ala Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr 
            420                 425                 430         


Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Gly 
        435                 440                 445             


Asn Asn Ser Thr Lys Glu Thr Phe Arg Pro Gly Gly Gly Asn Met Arg 
    450                 455                 460                 


Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu Val Lys 
465                 470                 475                 480 


Pro Leu Gly Ile Ala Pro Thr Glu Cys Asn Arg Thr Val Val Gln Arg 
                485                 490                 495     


Arg Arg Arg Arg Arg Ala Val Gly Leu Gly Ala Val Phe Leu Gly Phe 
            500                 505                 510         


Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Asn Thr Leu Thr 
        515                 520                 525             


Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn 
    530                 535                 540                 


Leu Leu Arg Ala Pro Glu Ala Gln Gln His Met Leu Gln Leu Gly Val 
545                 550                 555                 560 


Trp Gly Phe Lys Gln Leu Gln Ala Arg Val Leu Ala Ile Glu Arg Tyr 
                565                 570                 575     


Leu Glu Val Gln Gln Leu Leu Gly Met Trp Gly Cys Ser Gly Lys Leu 
            580                 585                 590         


Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr 
        595                 600                 605             


Tyr Asn Glu Ile Trp Asp Asn Met Thr Trp Met Gln Trp Asp Arg Glu 
    610                 615                 620                 


Ile Gly Asn Tyr Thr Asp Thr Ile Tyr Lys Leu Leu Glu Val Ser Gln 
625                 630                 635                 640 


Phe Gln Gln Glu Ile Asn Glu Lys Asp Asn Leu Thr Leu Asp 
                645                 650                 


<210>  21
<211>  617
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  21

Ala Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu 
1               5                   10                  15      


Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu Lys 
            20                  25                  30          


Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro 
        35                  40                  45              


Asn Pro Gln Glu Met Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met 
    50                  55                  60                  


Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu 
65                  70                  75                  80  


Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val 
                85                  90                  95      


Thr Leu Asn Cys Thr Asn Val Asn Val Thr Asn Thr Asn Asn Asn Asn 
            100                 105                 110         


Met Lys Glu Glu Met Lys Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile 
        115                 120                 125             


Arg Asp Lys Lys Gln Lys Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile 
    130                 135                 140                 


Val Pro Leu Asn Glu Asn Ser Ser Glu Tyr Arg Leu Ile Asn Cys Asn 
145                 150                 155                 160 


Thr Ser Thr Ile Thr Gln Ile Cys Pro Lys Val Ser Phe Asp Pro Ile 
                165                 170                 175     


Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn 
            180                 185                 190         


Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val 
        195                 200                 205             


Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu 
    210                 215                 220                 


Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu 
225                 230                 235                 240 


Thr Asp Asn Ala Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu 
                245                 250                 255     


Ile Asn Cys Thr Arg Pro Asn Asn Met Thr Arg Lys Ser Ile Arg Ile 
            260                 265                 270         


Gly Pro Gly Gln Thr Phe Tyr Ala Leu Gly Asp Ile Ile Gly Asp Ile 
        275                 280                 285             


Arg Gln Pro His Cys Asn Ile Ser Glu Ala Lys Trp Asn Lys Thr Leu 
    290                 295                 300                 


Gln Arg Val Lys Lys Lys Leu Lys Glu His Phe Pro Asn Lys Thr Ile 
305                 310                 315                 320 


Lys Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser 
                325                 330                 335     


Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Lys Leu Phe 
            340                 345                 350         


Asn Ser Thr Tyr Asn Asn Thr Thr Ser Asn Ser Thr Ile Thr Leu Pro 
        355                 360                 365             


Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala 
    370                 375                 380                 


Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser Asn Ile 
385                 390                 395                 400 


Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Asn Thr 
                405                 410                 415     


Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser 
            420                 425                 430         


Glu Leu Tyr Lys Tyr Lys Val Val Glu Ile Lys Pro Leu Gly Ile Ala 
        435                 440                 445             


Pro Thr Lys Cys Lys Arg Arg Val Val Glu Arg Arg Arg Arg Arg Arg 
    450                 455                 460                 


Ala Val Gly Leu Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly 
465                 470                 475                 480 


Ser Thr Met Gly Ala Ala Ser Asn Thr Leu Thr Val Gln Ala Arg Gln 
                485                 490                 495     


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro 
            500                 505                 510         


Glu Ala Gln Gln His Met Leu Gln Leu Gly Val Trp Gly Phe Lys Gln 
        515                 520                 525             


Leu Gln Ala Arg Val Leu Ala Ile Glu Arg Tyr Leu Glu Val Gln Gln 
    530                 535                 540                 


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala 
545                 550                 555                 560 


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Glu Asp Ile Trp 
                565                 570                 575     


Asp Asn Met Thr Trp Met Gln Trp Asp Arg Glu Ile Gly Asn Tyr Thr 
            580                 585                 590         


Asp Thr Ile Tyr Arg Leu Leu Glu Glu Ser Gln Phe Gln Gln Glu Ile 
        595                 600                 605             


Asn Glu Lys Asp Leu Leu Ala Leu Asp 
    610                 615         


<210>  22
<211>  646
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  22

Met Asp Ser Lys Gly Ser Ser Gln Lys Gly Ser Arg Leu Leu Leu Leu 
1               5                   10                  15      


Leu Val Val Ser Asn Leu Leu Leu Pro Gln Gly Val Leu Ala Asn Leu 
            20                  25                  30          


Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Lys Thr 
        35                  40                  45              


Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu Lys Glu Val His 
    50                  55                  60                  


Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln 
65                  70                  75                  80  


Glu Met Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn 
                85                  90                  95      


Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp Gln 
            100                 105                 110         


Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn 
        115                 120                 125             


Cys Thr Asn Val Asn Val Thr Asn Thr Asn Asn Asn Asn Met Lys Glu 
    130                 135                 140                 


Glu Met Lys Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg Asp Lys 
145                 150                 155                 160 


Lys Gln Lys Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu 
                165                 170                 175     


Asn Glu Asn Ser Ser Glu Tyr Arg Leu Ile Asn Cys Asn Thr Ser Thr 
            180                 185                 190         


Ile Thr Gln Ile Cys Pro Lys Val Ser Phe Asp Pro Ile Pro Ile His 
        195                 200                 205             


Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr 
    210                 215                 220                 


Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val Gln Cys Thr 
225                 230                 235                 240 


His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser 
                245                 250                 255     


Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr Asp Asn 
            260                 265                 270         


Ala Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu Ile Asn Cys 
        275                 280                 285             


Thr Arg Pro Asn Asn Met Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly 
    290                 295                 300                 


Gln Thr Phe Tyr Ala Leu Gly Asp Ile Ile Gly Asp Ile Arg Gln Pro 
305                 310                 315                 320 


His Cys Asn Ile Ser Glu Ala Lys Trp Asn Lys Thr Leu Gln Arg Val 
                325                 330                 335     


Lys Lys Lys Leu Lys Glu His Phe Pro Asn Lys Thr Ile Lys Phe Ala 
            340                 345                 350         


Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys 
        355                 360                 365             


Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Lys Leu Phe Asn Ser Thr 
    370                 375                 380                 


Tyr Asn Asn Thr Thr Ser Asn Ser Thr Ile Thr Leu Pro Cys Arg Ile 
385                 390                 395                 400 


Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Ala Met Tyr Ala 
                405                 410                 415     


Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu 
            420                 425                 430         


Leu Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Asn Thr Glu Thr Phe 
        435                 440                 445             


Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr 
    450                 455                 460                 


Lys Tyr Lys Val Val Glu Ile Lys Pro Leu Gly Ile Ala Pro Thr Lys 
465                 470                 475                 480 


Cys Lys Arg Arg Val Val Glu Arg Arg Arg Arg Arg Arg Ala Val Gly 
                485                 490                 495     


Leu Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met 
            500                 505                 510         


Gly Ala Ala Ser Asn Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser 
        515                 520                 525             


Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln 
    530                 535                 540                 


Gln His Met Leu Gln Leu Gly Val Trp Gly Phe Lys Gln Leu Gln Ala 
545                 550                 555                 560 


Arg Val Leu Ala Ile Glu Arg Tyr Leu Glu Val Gln Gln Leu Leu Gly 
                565                 570                 575     


Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala Val Pro Trp 
            580                 585                 590         


Asn Ser Ser Trp Ser Asn Lys Ser Gln Glu Asp Ile Trp Asp Asn Met 
        595                 600                 605             


Thr Trp Met Gln Trp Asp Arg Glu Ile Gly Asn Tyr Thr Asp Thr Ile 
    610                 615                 620                 


Tyr Arg Leu Leu Glu Glu Ser Gln Phe Gln Gln Glu Ile Asn Glu Lys 
625                 630                 635                 640 


Asp Leu Leu Ala Leu Asp 
                645     


<210>  23
<211>  625
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  23

Ala Gly Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Arg Glu 
1               5                   10                  15      


Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ser Tyr Glu Lys 
            20                  25                  30          


Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro 
        35                  40                  45              


Asn Pro Gln Glu Leu Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met 
    50                  55                  60                  


Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu 
65                  70                  75                  80  


Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val 
                85                  90                  95      


Thr Leu Asn Cys Ser Asp Ala Lys Val Asn Ala Thr Tyr Lys Gly Thr 
            100                 105                 110         


Arg Glu Glu Ile Lys Asn Cys Ser Phe Asn Ala Thr Thr Glu Leu Arg 
        115                 120                 125             


Asp Lys Lys Arg Arg Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val 
    130                 135                 140                 


Pro Leu Ser Gly Glu Gly Asn Asn Asn Ser Glu Tyr Arg Leu Ile Asn 
145                 150                 155                 160 


Cys Asn Thr Ser Val Cys Thr Gln Ile Cys Pro Lys Val Thr Phe Asp 
                165                 170                 175     


Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys 
            180                 185                 190         


Cys Asn Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser 
        195                 200                 205             


Thr Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu 
    210                 215                 220                 


Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu 
225                 230                 235                 240 


Asn Leu Thr Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Glu Ser 
                245                 250                 255     


Val Glu Ile Thr Cys Thr Arg Pro Asn Asn Met Thr Arg Lys Ser Val 
            260                 265                 270         


Arg Ile Gly Pro Gly Gln Thr Phe Tyr Ala Leu Gly Asp Ile Ile Gly 
        275                 280                 285             


Asp Ile Arg Gln Pro His Cys Asn Ile Ser Glu Ile Lys Trp Glu Lys 
    290                 295                 300                 


Thr Leu Gln Arg Val Ser Glu Lys Leu Arg Glu His Phe Asn Lys Thr 
305                 310                 315                 320 


Ile Ile Phe Asn Gln Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His 
                325                 330                 335     


Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Asp Leu 
            340                 345                 350         


Phe Phe Asn Lys Thr Phe Asn Glu Thr Tyr Ser Thr Gly Ser Asn Ser 
        355                 360                 365             


Thr Asn Ser Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn 
    370                 375                 380                 


Met Trp Gln Glu Val Gly Arg Cys Met Tyr Ala Pro Pro Ile Ala Gly 
385                 390                 395                 400 


Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp 
                405                 410                 415     


Gly Gly Gly Asn Asn Ser Thr Lys Glu Thr Phe Arg Pro Gly Gly Gly 
            420                 425                 430         


Asn Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val 
        435                 440                 445             


Glu Val Lys Pro Leu Gly Ile Ala Pro Thr Glu Cys Asn Arg Thr Val 
    450                 455                 460                 


Val Gln Arg Arg Arg Arg Arg Arg Ala Val Gly Leu Gly Ala Val Phe 
465                 470                 475                 480 


Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Asn 
                485                 490                 495     


Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln 
            500                 505                 510         


Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Met Leu Gln 
        515                 520                 525             


Leu Gly Val Trp Gly Phe Lys Gln Leu Gln Ala Arg Val Leu Ala Ile 
    530                 535                 540                 


Glu Arg Tyr Leu Glu Val Gln Gln Leu Leu Gly Met Trp Gly Cys Ser 
545                 550                 555                 560 


Gly Lys Leu Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser Trp Ser 
                565                 570                 575     


Asn Lys Thr Tyr Asn Glu Ile Trp Asp Asn Met Thr Trp Met Gln Trp 
            580                 585                 590         


Asp Arg Glu Ile Gly Asn Tyr Thr Asp Thr Ile Tyr Lys Leu Leu Glu 
        595                 600                 605             


Val Ser Gln Phe Gln Gln Glu Ile Asn Glu Lys Asp Asn Leu Thr Leu 
    610                 615                 620                 


Asp 
625 


<210>  24
<211>  617
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  24

Ala Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu 
1               5                   10                  15      


Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu Lys 
            20                  25                  30          


Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro 
        35                  40                  45              


Asn Pro Gln Glu Met Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met 
    50                  55                  60                  


Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu 
65                  70                  75                  80  


Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val 
                85                  90                  95      


Thr Leu Asn Cys Thr Asn Val Asn Val Thr Asn Thr Asn Asn Asn Asn 
            100                 105                 110         


Met Lys Glu Glu Met Lys Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile 
        115                 120                 125             


Arg Asp Lys Lys Gln Lys Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile 
    130                 135                 140                 


Val Pro Leu Asn Glu Asn Ser Ser Glu Tyr Arg Leu Ile Asn Cys Asn 
145                 150                 155                 160 


Thr Ser Thr Cys Thr Gln Ile Cys Pro Lys Val Ser Phe Asp Pro Ile 
                165                 170                 175     


Pro Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn 
            180                 185                 190         


Asn Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val 
        195                 200                 205             


Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu 
    210                 215                 220                 


Asn Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu 
225                 230                 235                 240 


Thr Asp Asn Ala Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu 
                245                 250                 255     


Ile Asn Cys Thr Arg Pro Asn Asn Met Thr Arg Lys Ser Ile Arg Ile 
            260                 265                 270         


Gly Pro Gly Gln Thr Phe Tyr Ala Leu Gly Asp Ile Ile Gly Asp Ile 
        275                 280                 285             


Arg Gln Pro His Cys Asn Ile Ser Glu Ala Lys Trp Asn Lys Thr Leu 
    290                 295                 300                 


Gln Arg Val Lys Lys Lys Leu Lys Glu His Phe Pro Asn Lys Thr Ile 
305                 310                 315                 320 


Lys Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser 
                325                 330                 335     


Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Lys Leu Phe 
            340                 345                 350         


Asn Ser Thr Tyr Asn Asn Thr Thr Ser Asn Ser Thr Ile Thr Leu Pro 
        355                 360                 365             


Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Cys 
    370                 375                 380                 


Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser Asn Ile 
385                 390                 395                 400 


Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Asn Thr 
                405                 410                 415     


Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser 
            420                 425                 430         


Glu Leu Tyr Lys Tyr Lys Val Val Glu Ile Lys Pro Leu Gly Ile Ala 
        435                 440                 445             


Pro Thr Lys Cys Lys Arg Arg Val Val Glu Arg Arg Arg Arg Arg Arg 
    450                 455                 460                 


Ala Val Gly Leu Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly 
465                 470                 475                 480 


Ser Thr Met Gly Ala Ala Ser Asn Thr Leu Thr Val Gln Ala Arg Gln 
                485                 490                 495     


Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro 
            500                 505                 510         


Glu Ala Gln Gln His Met Leu Gln Leu Gly Val Trp Gly Phe Lys Gln 
        515                 520                 525             


Leu Gln Ala Arg Val Leu Ala Ile Glu Arg Tyr Leu Glu Val Gln Gln 
    530                 535                 540                 


Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala 
545                 550                 555                 560 


Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Ser Gln Glu Asp Ile Trp 
                565                 570                 575     


Asp Asn Met Thr Trp Met Gln Trp Asp Arg Glu Ile Gly Asn Tyr Thr 
            580                 585                 590         


Asp Thr Ile Tyr Arg Leu Leu Glu Glu Ser Gln Phe Gln Gln Glu Ile 
        595                 600                 605             


Asn Glu Lys Asp Leu Leu Ala Leu Asp 
    610                 615         


<210>  25
<211>  654
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  25

Met Asp Ser Lys Gly Ser Ser Gln Lys Gly Ser Arg Leu Leu Leu Leu 
1               5                   10                  15      


Leu Val Val Ser Asn Leu Leu Leu Pro Gln Gly Val Leu Ala Gly Leu 
            20                  25                  30          


Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Arg Glu Ala Lys Thr 
        35                  40                  45              


Thr Leu Phe Cys Ala Ser Asp Ala Lys Ser Tyr Glu Lys Glu Val His 
    50                  55                  60                  


Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln 
65                  70                  75                  80  


Glu Leu Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn 
                85                  90                  95      


Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp Gln 
            100                 105                 110         


Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn 
        115                 120                 125             


Cys Ser Asp Ala Lys Val Asn Ala Thr Tyr Lys Gly Thr Arg Glu Glu 
    130                 135                 140                 


Ile Lys Asn Cys Ser Phe Asn Ala Thr Thr Glu Leu Arg Asp Lys Lys 
145                 150                 155                 160 


Arg Arg Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu Ser 
                165                 170                 175     


Gly Glu Gly Asn Asn Asn Ser Glu Tyr Arg Leu Ile Asn Cys Asn Thr 
            180                 185                 190         


Ser Val Cys Thr Gln Ile Cys Pro Lys Val Thr Phe Asp Pro Ile Pro 
        195                 200                 205             


Ile His Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
    210                 215                 220                 


Lys Thr Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val Gln 
225                 230                 235                 240 


Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
                245                 250                 255     


Gly Ser Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr 
            260                 265                 270         


Asp Asn Val Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu Ile 
        275                 280                 285             


Thr Cys Thr Arg Pro Asn Asn Met Thr Arg Lys Ser Val Arg Ile Gly 
    290                 295                 300                 


Pro Gly Gln Thr Phe Tyr Ala Leu Gly Asp Ile Ile Gly Asp Ile Arg 
305                 310                 315                 320 


Gln Pro His Cys Asn Ile Ser Glu Ile Lys Trp Glu Lys Thr Leu Gln 
                325                 330                 335     


Arg Val Ser Glu Lys Leu Arg Glu His Phe Asn Lys Thr Ile Ile Phe 
            340                 345                 350         


Asn Gln Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn 
        355                 360                 365             


Cys Gly Gly Glu Phe Phe Tyr Cys Asn Thr Ser Asp Leu Phe Phe Asn 
    370                 375                 380                 


Lys Thr Phe Asn Glu Thr Tyr Ser Thr Gly Ser Asn Ser Thr Asn Ser 
385                 390                 395                 400 


Thr Ile Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln 
                405                 410                 415     


Glu Val Gly Arg Cys Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr 
            420                 425                 430         


Cys Lys Ser Asn Ile Thr Gly Leu Leu Leu Thr Arg Asp Gly Gly Gly 
        435                 440                 445             


Asn Asn Ser Thr Lys Glu Thr Phe Arg Pro Gly Gly Gly Asn Met Arg 
    450                 455                 460                 


Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu Val Lys 
465                 470                 475                 480 


Pro Leu Gly Ile Ala Pro Thr Glu Cys Asn Arg Thr Val Val Gln Arg 
                485                 490                 495     


Arg Arg Arg Arg Arg Ala Val Gly Leu Gly Ala Val Phe Leu Gly Phe 
            500                 505                 510         


Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Asn Thr Leu Thr 
        515                 520                 525             


Val Gln Ala Arg Gln Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn 
    530                 535                 540                 


Leu Leu Arg Ala Pro Glu Ala Gln Gln His Met Leu Gln Leu Gly Val 
545                 550                 555                 560 


Trp Gly Phe Lys Gln Leu Gln Ala Arg Val Leu Ala Ile Glu Arg Tyr 
                565                 570                 575     


Leu Glu Val Gln Gln Leu Leu Gly Met Trp Gly Cys Ser Gly Lys Leu 
            580                 585                 590         


Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Lys Thr 
        595                 600                 605             


Tyr Asn Glu Ile Trp Asp Asn Met Thr Trp Met Gln Trp Asp Arg Glu 
    610                 615                 620                 


Ile Gly Asn Tyr Thr Asp Thr Ile Tyr Lys Leu Leu Glu Val Ser Gln 
625                 630                 635                 640 


Phe Gln Gln Glu Ile Asn Glu Lys Asp Asn Leu Thr Leu Asp 
                645                 650                 


<210>  26
<211>  646
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 envelope protein

<400>  26

Met Asp Ser Lys Gly Ser Ser Gln Lys Gly Ser Arg Leu Leu Leu Leu 
1               5                   10                  15      


Leu Val Val Ser Asn Leu Leu Leu Pro Gln Gly Val Leu Ala Asn Leu 
            20                  25                  30          


Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Glu Ala Lys Thr 
        35                  40                  45              


Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu Lys Glu Val His 
    50                  55                  60                  


Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn Pro Gln 
65                  70                  75                  80  


Glu Met Val Leu Glu Asn Val Thr Glu Asn Phe Asn Met Trp Lys Asn 
                85                  90                  95      


Asp Met Val Asp Gln Met His Glu Asp Ile Ile Ser Leu Trp Asp Gln 
            100                 105                 110         


Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr Leu Asn 
        115                 120                 125             


Cys Thr Asn Val Asn Val Thr Asn Thr Asn Asn Asn Asn Met Lys Glu 
    130                 135                 140                 


Glu Met Lys Asn Cys Ser Phe Asn Thr Thr Thr Glu Ile Arg Asp Lys 
145                 150                 155                 160 


Lys Gln Lys Glu Tyr Ala Leu Phe Tyr Arg Leu Asp Ile Val Pro Leu 
                165                 170                 175     


Asn Glu Asn Ser Ser Glu Tyr Arg Leu Ile Asn Cys Asn Thr Ser Thr 
            180                 185                 190         


Cys Thr Gln Ile Cys Pro Lys Val Ser Phe Asp Pro Ile Pro Ile His 
        195                 200                 205             


Tyr Cys Ala Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn Lys Thr 
    210                 215                 220                 


Phe Asn Gly Thr Gly Pro Cys Asn Asn Val Ser Thr Val Gln Cys Thr 
225                 230                 235                 240 


His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser 
                245                 250                 255     


Leu Ala Glu Glu Glu Ile Ile Ile Arg Ser Glu Asn Leu Thr Asp Asn 
            260                 265                 270         


Ala Lys Thr Ile Ile Val His Leu Asn Glu Ser Val Glu Ile Asn Cys 
        275                 280                 285             


Thr Arg Pro Asn Asn Met Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly 
    290                 295                 300                 


Gln Thr Phe Tyr Ala Leu Gly Asp Ile Ile Gly Asp Ile Arg Gln Pro 
305                 310                 315                 320 


His Cys Asn Ile Ser Glu Ala Lys Trp Asn Lys Thr Leu Gln Arg Val 
                325                 330                 335     


Lys Lys Lys Leu Lys Glu His Phe Pro Asn Lys Thr Ile Lys Phe Ala 
            340                 345                 350         


Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe Asn Cys 
        355                 360                 365             


Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Lys Leu Phe Asn Ser Thr 
    370                 375                 380                 


Tyr Asn Asn Thr Thr Ser Asn Ser Thr Ile Thr Leu Pro Cys Arg Ile 
385                 390                 395                 400 


Lys Gln Ile Ile Asn Met Trp Gln Glu Val Gly Arg Cys Met Tyr Ala 
                405                 410                 415     


Pro Pro Ile Ala Gly Asn Ile Thr Cys Lys Ser Asn Ile Thr Gly Leu 
            420                 425                 430         


Leu Leu Thr Arg Asp Gly Gly Asn Asn Asn Asn Asn Thr Glu Thr Phe 
        435                 440                 445             


Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr 
    450                 455                 460                 


Lys Tyr Lys Val Val Glu Ile Lys Pro Leu Gly Ile Ala Pro Thr Lys 
465                 470                 475                 480 


Cys Lys Arg Arg Val Val Glu Arg Arg Arg Arg Arg Arg Ala Val Gly 
                485                 490                 495     


Leu Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met 
            500                 505                 510         


Gly Ala Ala Ser Asn Thr Leu Thr Val Gln Ala Arg Gln Leu Leu Ser 
        515                 520                 525             


Gly Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln 
    530                 535                 540                 


Gln His Met Leu Gln Leu Gly Val Trp Gly Phe Lys Gln Leu Gln Ala 
545                 550                 555                 560 


Arg Val Leu Ala Ile Glu Arg Tyr Leu Glu Val Gln Gln Leu Leu Gly 
                565                 570                 575     


Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Ala Val Pro Trp 
            580                 585                 590         


Asn Ser Ser Trp Ser Asn Lys Ser Gln Glu Asp Ile Trp Asp Asn Met 
        595                 600                 605             


Thr Trp Met Gln Trp Asp Arg Glu Ile Gly Asn Tyr Thr Asp Thr Ile 
    610                 615                 620                 


Tyr Arg Leu Leu Glu Glu Ser Gln Phe Gln Gln Glu Ile Asn Glu Lys 
625                 630                 635                 640 


Asp Leu Leu Ala Leu Asp 
                645     


<210>  27
<211>  7
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant furin cleavage site

<400>  27

Gly Arg Arg Arg Arg Arg Arg 
1               5           


<210>  28
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant furin cleavage site

<400>  28

Gly Gly Ser Gly Arg Arg Arg Arg Arg Arg 
1               5                   10  


<210>  29
<211>  10
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant furin cleavage site

<400>  29

Gly Arg Arg Arg Arg Arg Arg Arg Arg Arg 
1               5                   10  


<210>  30
<211>  18
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant furin cleavage site

<400>  30

Gly Asn Ser Thr His Lys Gln Leu Thr His His Met Arg Arg Arg Arg 
1               5                   10                  15      


Arg Arg 
        


<210>  31
<211>  625
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 Env sequence

<400>  31

Ala Val Glu Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp 
1               5                   10                  15      


Lys Asp Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr 
            20                  25                  30          


Asp Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr 
        35                  40                  45              


Asp Pro Asn Pro Gln Glu Met Leu Leu Asp Asn Val Thr Glu Asn Phe 
    50                  55                  60                  


Asn Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Val Ile 
65                  70                  75                  80  


Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu 
                85                  90                  95      


Cys Val Thr Leu Glu Cys Thr Asp Ser Ser Asn Gln Thr His Tyr Asn 
            100                 105                 110         


Glu Ser Met Gln Glu Ile Lys Asn Cys Thr Phe Asn Val Thr Thr Glu 
        115                 120                 125             


Ile Arg Asp Arg Lys Gln Arg Val Gln Ala Leu Phe Tyr Lys Leu Asp 
    130                 135                 140                 


Ile Val Ser Leu Glu Lys Asn Ser Ser Thr Tyr Arg Leu Ile Asn Cys 
145                 150                 155                 160 


Asn Thr Ser Ala Cys Thr Gln Ala Cys Pro Lys Val Thr Phe Asp Pro 
                165                 170                 175     


Ile Pro Ile His Tyr Cys Thr Pro Ala Gly Tyr Ala Ile Leu Lys Cys 
            180                 185                 190         


Asn Asn Glu Thr Phe Asn Gly Thr Gly Pro Cys Arg Asn Val Ser Thr 
        195                 200                 205             


Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu 
    210                 215                 220                 


Leu Asn Gly Ser Leu Ala Glu Lys Asp Ile Met Ile Arg Ser Glu Asn 
225                 230                 235                 240 


Leu Thr Asp Asn Ala Lys Thr Ile Ile Val His Leu Asn Gln Thr Val 
                245                 250                 255     


Glu Ile Val Cys Ile Arg Pro Asn Asn Met Thr Arg Gln Ser Ile Arg 
            260                 265                 270         


Ile Gly Pro Gly Gln Val Phe Tyr Ala Leu Gly Asp Ile Ile Gly Asp 
        275                 280                 285             


Ile Arg Gln Pro Tyr Cys Thr Ile Asn Thr Thr Ala Trp Asn Glu Thr 
    290                 295                 300                 


Leu Gln Arg Val Ser Lys Lys Leu Ala Glu His Phe Pro Asn Lys Thr 
305                 310                 315                 320 


Ile Arg Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His 
                325                 330                 335     


Ser Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Gly Leu 
            340                 345                 350         


Phe Asn Ser Thr Tyr Met Thr Asn Gly Thr Phe Thr Tyr Lys Leu Asn 
        355                 360                 365             


Asp Thr Asn Ile Thr Ile Pro Cys Arg Ile Lys Gln Ile Ile Asn Met 
    370                 375                 380                 


Trp Gln Glu Val Gly Arg Cys Met Tyr Ala Pro Pro Ile Ala Gly Asn 
385                 390                 395                 400 


Ile Thr Cys Lys Ser Asn Ile Thr Gly Met Leu Leu Val Arg Asp Gly 
                405                 410                 415     


Gly Lys Asn Glu Asn Ser Thr Glu Glu Thr Phe Arg Pro Gly Gly Gly 
            420                 425                 430         


Asn Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val 
        435                 440                 445             


Glu Ile Lys Pro Leu Gly Val Ala Pro Thr Lys Cys Lys Arg Arg Val 
    450                 455                 460                 


Val Gly Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala Val Phe 
465                 470                 475                 480 


Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met 
                485                 490                 495     


Thr Leu Thr Val Gln Ala Arg Asn Leu Leu Ser Gly Ile Val Gln Gln 
            500                 505                 510         


Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Leu Leu Lys 
        515                 520                 525             


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val 
    530                 535                 540                 


Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser 
545                 550                 555                 560 


Gly Lys Leu Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser Trp Ser 
                565                 570                 575     


Asn Arg Asn Leu Ser Glu Ile Trp Asp Asn Met Thr Trp Leu Gln Trp 
            580                 585                 590         


Asp Lys Glu Ile Ser Asn Tyr Thr Gln Ile Ile Tyr Gly Leu Leu Glu 
        595                 600                 605             


Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln Asp Leu Leu Ala Leu 
    610                 615                 620                 


Asp 
625 


<210>  32
<211>  645
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Recombinant HIV-1 Env sequence

<400>  32

Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Thr Glu Lys His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Ile His Leu Glu Asn Val Thr Glu Glu Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Gln Cys Thr Asn Val Thr Asn Asn Ile Thr Asp Asp Met 
            100                 105                 110         


Arg Gly Glu Leu Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Val Tyr Ser Leu Phe Tyr Arg Leu Asp Val Val 
    130                 135                 140                 


Gln Ile Asn Glu Asn Gln Gly Asn Arg Ser Asn Asn Ser Asn Lys Glu 
145                 150                 155                 160 


Tyr Arg Leu Ile Asn Cys Asn Thr Ser Ala Cys Thr Gln Ala Cys Pro 
                165                 170                 175     


Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly 
            180                 185                 190         


Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro 
        195                 200                 205             


Cys Pro Ser Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val 
    210                 215                 220                 


Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val 
225                 230                 235                 240 


Met Ile Arg Ser Glu Asn Ile Thr Asn Asn Ala Lys Asn Ile Leu Val 
                245                 250                 255     


Gln Phe Asn Thr Pro Val Gln Ile Asn Cys Thr Arg Pro Asn Asn Met 
            260                 265                 270         


Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Ala Phe Tyr Ala Leu 
        275                 280                 285             


Gly Asp Ile Ile Gly Asp Ile Arg Gln Pro His Cys Asn Val Ser Lys 
    290                 295                 300                 


Ala Thr Trp Asn Glu Thr Leu Gly Lys Val Val Lys Gln Leu Arg Lys 
305                 310                 315                 320 


His Phe Gly Asn Asn Thr Ile Ile Arg Phe Ala Asn Ser Ser Gly Gly 
                325                 330                 335     


Asp Leu Glu Val Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe 
            340                 345                 350         


Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Thr Trp Ile Ser Asn Thr 
        355                 360                 365             


Ser Val Gln Gly Ser Asn Ser Thr Gly Ser Asn Asp Ser Ile Thr Leu 
    370                 375                 380                 


Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Ile Gly Gln 
385                 390                 395                 400 


Cys Met Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Val Ser Asn 
                405                 410                 415     


Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Ser Thr Asn Ser Thr 
            420                 425                 430         


Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg 
        435                 440                 445             


Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val 
    450                 455                 460                 


Ala Pro Thr Arg Cys Lys Arg Arg Val Val Gly Asn Ser Thr His Lys 
465                 470                 475                 480 


Gln Leu Thr His His Met Arg Arg Arg Arg Arg Arg Ala Val Gly Ile 
                485                 490                 495     


Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly 
            500                 505                 510         


Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Asn Leu Leu Ser Gly 
        515                 520                 525             


Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln 
    530                 535                 540                 


His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg 
545                 550                 555                 560 


Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile 
                565                 570                 575     


Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Asn Val Pro Trp Asn 
            580                 585                 590         


Ser Ser Trp Ser Asn Arg Asn Leu Ser Glu Ile Trp Asp Asn Met Thr 
        595                 600                 605             


Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr Gln Ile Ile Tyr 
    610                 615                 620                 


Gly Leu Leu Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln Asp 
625                 630                 635                 640 


Leu Leu Ala Leu Asp 
                645 


<210>  33
<211>  625
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant HIV-1 Env sequence

<400>  33

Ala Val Glu Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp 
1               5                   10                  15      


Lys Asp Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr 
            20                  25                  30          


Asp Thr Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr 
        35                  40                  45              


Asp Pro Asn Pro Gln Glu Met Leu Leu Asp Asn Val Thr Glu Asn Phe 
    50                  55                  60                  


Asn Met Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Val Ile 
65                  70                  75                  80  


Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu 
                85                  90                  95      


Cys Val Thr Leu Glu Cys Thr Asp Ser Ser Asn Gln Thr His Tyr Asn 
            100                 105                 110         


Glu Ser Met Gln Glu Ile Lys Asn Cys Thr Phe Asn Val Thr Thr Glu 
        115                 120                 125             


Ile Arg Asp Arg Lys Gln Arg Val Gln Ala Leu Phe Tyr Lys Leu Asp 
    130                 135                 140                 


Ile Val Ser Leu Glu Lys Asn Ser Ser Thr Tyr Arg Leu Ile Asn Cys 
145                 150                 155                 160 


Asn Thr Ser Ala Cys Thr Gln Ala Cys Pro Lys Val Thr Phe Asp Pro 
                165                 170                 175     


Ile Pro Ile His Tyr Cys Thr Pro Ala Gly Tyr Ala Ile Leu Lys Cys 
            180                 185                 190         


Asn Asn Glu Thr Phe Asn Gly Thr Gly Pro Cys Arg Asn Val Ser Thr 
        195                 200                 205             


Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu 
    210                 215                 220                 


Leu Asn Gly Ser Leu Ala Glu Lys Asp Ile Met Ile Arg Ser Glu Asn 
225                 230                 235                 240 


Leu Thr Asp Asn Ala Lys Thr Ile Ile Val His Leu Asn Gln Thr Val 
                245                 250                 255     


Glu Ile Val Cys Ile Arg Pro Asn Asn Asn Thr Arg Gln Ser Ile Arg 
            260                 265                 270         


Ile Gly Pro Gly Gln Val Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp 
        275                 280                 285             


Ile Arg Gln Ala Tyr Cys Thr Ile Asn Thr Thr Ala Trp Asn Glu Thr 
    290                 295                 300                 


Leu Gln Arg Val Ser Lys Lys Leu Ala Glu His Phe Pro Asn Lys Thr 
305                 310                 315                 320 


Ile Arg Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His 
                325                 330                 335     


Ser Phe Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Gly Leu 
            340                 345                 350         


Phe Asn Ser Thr Tyr Met Thr Asn Gly Thr Phe Thr Tyr Lys Leu Asn 
        355                 360                 365             


Asp Thr Asn Ile Thr Ile Pro Cys Arg Ile Lys Gln Ile Ile Asn Met 
    370                 375                 380                 


Trp Gln Glu Val Gly Arg Cys Met Tyr Ala Pro Pro Ile Ala Gly Asn 
385                 390                 395                 400 


Ile Thr Cys Lys Ser Asn Ile Thr Gly Met Leu Leu Val Arg Asp Gly 
                405                 410                 415     


Gly Lys Asn Glu Asn Ser Thr Glu Glu Thr Phe Arg Pro Gly Gly Gly 
            420                 425                 430         


Asn Met Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val 
        435                 440                 445             


Glu Ile Lys Pro Leu Gly Val Ala Pro Thr Lys Cys Lys Arg Arg Val 
    450                 455                 460                 


Val Gly Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala Val Phe 
465                 470                 475                 480 


Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met 
                485                 490                 495     


Thr Leu Thr Val Gln Ala Arg Asn Leu Leu Ser Gly Ile Val Gln Gln 
            500                 505                 510         


Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Leu Leu Lys 
        515                 520                 525             


Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val 
    530                 535                 540                 


Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser 
545                 550                 555                 560 


Gly Lys Leu Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser Trp Ser 
                565                 570                 575     


Asn Arg Asn Leu Ser Glu Ile Trp Asp Asn Met Thr Trp Leu Gln Trp 
            580                 585                 590         


Asp Lys Glu Ile Ser Asn Tyr Thr Gln Ile Ile Tyr Gly Leu Leu Glu 
        595                 600                 605             


Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln Asp Leu Leu Ala Leu 
    610                 615                 620                 


Asp 
625 


<210>  34
<211>  645
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant HIV-1 Env sequence

<400>  34

Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys 
1               5                   10                  15      


Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu 
            20                  25                  30          


Thr Glu Lys His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp 
        35                  40                  45              


Pro Asn Pro Gln Glu Ile His Leu Glu Asn Val Thr Glu Glu Phe Asn 
    50                  55                  60                  


Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser 
65                  70                  75                  80  


Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys 
                85                  90                  95      


Val Thr Leu Gln Cys Thr Asn Val Thr Asn Asn Ile Thr Asp Asp Met 
            100                 105                 110         


Arg Gly Glu Leu Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg 
        115                 120                 125             


Asp Lys Lys Gln Lys Val Tyr Ser Leu Phe Tyr Arg Leu Asp Val Val 
    130                 135                 140                 


Gln Ile Asn Glu Asn Gln Gly Asn Arg Ser Asn Asn Ser Asn Lys Glu 
145                 150                 155                 160 


Tyr Arg Leu Ile Asn Cys Asn Thr Ser Ala Cys Thr Gln Ala Cys Pro 
                165                 170                 175     


Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly 
            180                 185                 190         


Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro 
        195                 200                 205             


Cys Pro Ser Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val 
    210                 215                 220                 


Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val 
225                 230                 235                 240 


Met Ile Arg Ser Glu Asn Ile Thr Asn Asn Ala Lys Asn Ile Leu Val 
                245                 250                 255     


Gln Phe Asn Thr Pro Val Gln Ile Asn Cys Thr Arg Pro Asn Asn Asn 
            260                 265                 270         


Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Ala Phe Tyr Ala Thr 
        275                 280                 285             


Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Val Ser Lys 
    290                 295                 300                 


Ala Thr Trp Asn Glu Thr Leu Gly Lys Val Val Lys Gln Leu Arg Lys 
305                 310                 315                 320 


His Phe Gly Asn Asn Thr Ile Ile Arg Phe Ala Asn Ser Ser Gly Gly 
                325                 330                 335     


Asp Leu Glu Val Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe 
            340                 345                 350         


Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Thr Trp Ile Ser Asn Thr 
        355                 360                 365             


Ser Val Gln Gly Ser Asn Ser Thr Gly Ser Asn Asp Ser Ile Thr Leu 
    370                 375                 380                 


Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Ile Gly Gln 
385                 390                 395                 400 


Cys Met Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Val Ser Asn 
                405                 410                 415     


Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Ser Thr Asn Ser Thr 
            420                 425                 430         


Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg 
        435                 440                 445             


Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val 
    450                 455                 460                 


Ala Pro Thr Arg Cys Lys Arg Arg Val Val Gly Asn Ser Thr His Lys 
465                 470                 475                 480 


Gln Leu Thr His His Met Arg Arg Arg Arg Arg Arg Ala Val Gly Ile 
                485                 490                 495     


Gly Ala Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly 
            500                 505                 510         


Ala Ala Ser Met Thr Leu Thr Val Gln Ala Arg Asn Leu Leu Ser Gly 
        515                 520                 525             


Ile Val Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln 
    530                 535                 540                 


His Leu Leu Lys Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg 
545                 550                 555                 560 


Val Leu Ala Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile 
                565                 570                 575     


Trp Gly Cys Ser Gly Lys Leu Ile Cys Cys Thr Asn Val Pro Trp Asn 
            580                 585                 590         


Ser Ser Trp Ser Asn Arg Asn Leu Ser Glu Ile Trp Asp Asn Met Thr 
        595                 600                 605             


Trp Leu Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr Gln Ile Ile Tyr 
    610                 615                 620                 


Gly Leu Leu Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln Asp 
625                 630                 635                 640 


Leu Leu Ala Leu Asp 
                645 


<210>  35
<211>  655
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant HIV-1 Env sequence

<400>  35

Met Asp Ser Lys Gly Ser Ser Gln Lys Gly Ser Arg Leu Leu Leu Leu 
1               5                   10                  15      


Leu Val Val Ser Asn Leu Leu Leu Pro Gln Gly Val Leu Ala Ala Val 
            20                  25                  30          


Glu Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Asp 
        35                  40                  45              


Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr 
    50                  55                  60                  


Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro 
65                  70                  75                  80  


Asn Pro Gln Glu Met Leu Leu Asp Asn Val Thr Glu Asn Phe Asn Met 
                85                  90                  95      


Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Val Ile Ser Leu 
            100                 105                 110         


Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val 
        115                 120                 125             


Thr Leu Glu Cys Thr Asp Ser Ser Asn Gln Thr His Tyr Asn Glu Ser 
    130                 135                 140                 


Met Gln Glu Ile Lys Asn Cys Thr Phe Asn Val Thr Thr Glu Ile Arg 
145                 150                 155                 160 


Asp Arg Lys Gln Arg Val Gln Ala Leu Phe Tyr Lys Leu Asp Ile Val 
                165                 170                 175     


Ser Leu Glu Lys Asn Ser Ser Thr Tyr Arg Leu Ile Asn Cys Asn Thr 
            180                 185                 190         


Ser Ala Cys Thr Gln Ala Cys Pro Lys Val Thr Phe Asp Pro Ile Pro 
        195                 200                 205             


Ile His Tyr Cys Thr Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
    210                 215                 220                 


Glu Thr Phe Asn Gly Thr Gly Pro Cys Arg Asn Val Ser Thr Val Gln 
225                 230                 235                 240 


Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
                245                 250                 255     


Gly Ser Leu Ala Glu Lys Asp Ile Met Ile Arg Ser Glu Asn Leu Thr 
            260                 265                 270         


Asp Asn Ala Lys Thr Ile Ile Val His Leu Asn Gln Thr Val Glu Ile 
        275                 280                 285             


Val Cys Ile Arg Pro Asn Asn Met Thr Arg Gln Ser Ile Arg Ile Gly 
    290                 295                 300                 


Pro Gly Gln Val Phe Tyr Ala Leu Gly Asp Ile Ile Gly Asp Ile Arg 
305                 310                 315                 320 


Gln Pro Tyr Cys Thr Ile Asn Thr Thr Ala Trp Asn Glu Thr Leu Gln 
                325                 330                 335     


Arg Val Ser Lys Lys Leu Ala Glu His Phe Pro Asn Lys Thr Ile Arg 
            340                 345                 350         


Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe 
        355                 360                 365             


Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Gly Leu Phe Asn 
    370                 375                 380                 


Ser Thr Tyr Met Thr Asn Gly Thr Phe Thr Tyr Lys Leu Asn Asp Thr 
385                 390                 395                 400 


Asn Ile Thr Ile Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln 
                405                 410                 415     


Glu Val Gly Arg Cys Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr 
            420                 425                 430         


Cys Lys Ser Asn Ile Thr Gly Met Leu Leu Val Arg Asp Gly Gly Lys 
        435                 440                 445             


Asn Glu Asn Ser Thr Glu Glu Thr Phe Arg Pro Gly Gly Gly Asn Met 
    450                 455                 460                 


Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu Ile 
465                 470                 475                 480 


Lys Pro Leu Gly Val Ala Pro Thr Lys Cys Lys Arg Arg Val Val Gly 
                485                 490                 495     


Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly 
            500                 505                 510         


Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu 
        515                 520                 525             


Thr Val Gln Ala Arg Asn Leu Leu Ser Gly Ile Val Gln Gln Gln Ser 
    530                 535                 540                 


Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Leu Leu Lys Leu Thr 
545                 550                 555                 560 


Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg 
                565                 570                 575     


Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys 
            580                 585                 590         


Leu Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Arg 
        595                 600                 605             


Asn Leu Ser Glu Ile Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys 
    610                 615                 620                 


Glu Ile Ser Asn Tyr Thr Gln Ile Ile Tyr Gly Leu Leu Glu Glu Ser 
625                 630                 635                 640 


Gln Asn Gln Gln Glu Lys Asn Glu Gln Asp Leu Leu Ala Leu Asp 
                645                 650                 655 


<210>  36
<211>  675
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant HIV-1 Env sequence

<400>  36

Met Asp Ser Lys Gly Ser Ser Gln Lys Gly Ser Arg Leu Leu Leu Leu 
1               5                   10                  15      


Leu Val Val Ser Asn Leu Leu Leu Pro Gln Gly Val Leu Ala Ala Glu 
            20                  25                  30          


Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Asp Ala 
        35                  40                  45              


Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu Thr Glu 
    50                  55                  60                  


Lys His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Ile His Leu Glu Asn Val Thr Glu Glu Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
        115                 120                 125             


Leu Gln Cys Thr Asn Val Thr Asn Asn Ile Thr Asp Asp Met Arg Gly 
    130                 135                 140                 


Glu Leu Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg Asp Lys 
145                 150                 155                 160 


Lys Gln Lys Val Tyr Ser Leu Phe Tyr Arg Leu Asp Val Val Gln Ile 
                165                 170                 175     


Asn Glu Asn Gln Gly Asn Arg Ser Asn Asn Ser Asn Lys Glu Tyr Arg 
            180                 185                 190         


Leu Ile Asn Cys Asn Thr Ser Ala Cys Thr Gln Ala Cys Pro Lys Val 
        195                 200                 205             


Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe Ala 
    210                 215                 220                 


Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro Cys Pro 
225                 230                 235                 240 


Ser Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser 
                245                 250                 255     


Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val Met Ile 
            260                 265                 270         


Arg Ser Glu Asn Ile Thr Asn Asn Ala Lys Asn Ile Leu Val Gln Phe 
        275                 280                 285             


Asn Thr Pro Val Gln Ile Asn Cys Thr Arg Pro Asn Asn Met Thr Arg 
    290                 295                 300                 


Lys Ser Ile Arg Ile Gly Pro Gly Gln Ala Phe Tyr Ala Leu Gly Asp 
305                 310                 315                 320 


Ile Ile Gly Asp Ile Arg Gln Pro His Cys Asn Val Ser Lys Ala Thr 
                325                 330                 335     


Trp Asn Glu Thr Leu Gly Lys Val Val Lys Gln Leu Arg Lys His Phe 
            340                 345                 350         


Gly Asn Asn Thr Ile Ile Arg Phe Ala Asn Ser Ser Gly Gly Asp Leu 
        355                 360                 365             


Glu Val Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys 
    370                 375                 380                 


Asn Thr Ser Gly Leu Phe Asn Ser Thr Trp Ile Ser Asn Thr Ser Val 
385                 390                 395                 400 


Gln Gly Ser Asn Ser Thr Gly Ser Asn Asp Ser Ile Thr Leu Pro Cys 
                405                 410                 415     


Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Ile Gly Gln Cys Met 
            420                 425                 430         


Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Val Ser Asn Ile Thr 
        435                 440                 445             


Gly Leu Ile Leu Thr Arg Asp Gly Gly Ser Thr Asn Ser Thr Thr Glu 
    450                 455                 460                 


Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
465                 470                 475                 480 


Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro 
                485                 490                 495     


Thr Arg Cys Lys Arg Arg Val Val Gly Asn Ser Thr His Lys Gln Leu 
            500                 505                 510         


Thr His His Met Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala 
        515                 520                 525             


Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala 
    530                 535                 540                 


Ser Met Thr Leu Thr Val Gln Ala Arg Asn Leu Leu Ser Gly Ile Val 
545                 550                 555                 560 


Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Leu 
                565                 570                 575     


Leu Lys Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu 
            580                 585                 590         


Ala Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly 
        595                 600                 605             


Cys Ser Gly Lys Leu Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser 
    610                 615                 620                 


Trp Ser Asn Arg Asn Leu Ser Glu Ile Trp Asp Asn Met Thr Trp Leu 
625                 630                 635                 640 


Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr Gln Ile Ile Tyr Gly Leu 
                645                 650                 655     


Leu Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln Asp Leu Leu 
            660                 665                 670         


Ala Leu Asp 
        675 


<210>  37
<211>  654
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant HIV-1 Env sequence

<400>  37

Met Asp Ser Lys Gly Ser Ser Gln Lys Gly Ser Arg Leu Leu Leu Leu 
1               5                   10                  15      


Leu Val Val Ser Asn Leu Leu Leu Pro Gln Gly Val Leu Ala Ala Val 
            20                  25                  30          


Glu Lys Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Asp 
        35                  40                  45              


Ala Lys Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Asp Thr 
    50                  55                  60                  


Glu Val His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro 
65                  70                  75                  80  


Asn Pro Gln Glu Met Leu Leu Asp Asn Val Thr Glu Asn Phe Asn Met 
                85                  90                  95      


Trp Lys Asn Asp Met Val Asp Gln Met His Glu Asp Val Ile Ser Leu 
            100                 105                 110         


Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val 
        115                 120                 125             


Thr Leu Glu Cys Thr Asp Ser Ser Asn Gln Thr His Tyr Asn Glu Ser 
    130                 135                 140                 


Met Gln Glu Ile Lys Asn Cys Thr Phe Asn Val Thr Thr Glu Ile Arg 
145                 150                 155                 160 


Asp Arg Lys Gln Arg Val Gln Ala Leu Phe Tyr Lys Leu Asp Ile Val 
                165                 170                 175     


Ser Leu Glu Lys Asn Ser Ser Thr Tyr Arg Leu Ile Asn Cys Asn Thr 
            180                 185                 190         


Ser Ala Cys Thr Gln Ala Cys Pro Lys Val Thr Phe Asp Pro Ile Pro 
        195                 200                 205             


Ile His Tyr Cys Thr Pro Ala Gly Tyr Ala Ile Leu Lys Cys Asn Asn 
    210                 215                 220                 


Glu Thr Phe Asn Gly Thr Gly Pro Cys Arg Asn Val Ser Thr Val Gln 
225                 230                 235                 240 


Cys Thr His Gly Ile Lys Pro Val Val Ser Thr Gln Leu Leu Leu Asn 
                245                 250                 255     


Gly Ser Leu Ala Glu Lys Asp Ile Met Ile Arg Ser Glu Asn Leu Thr 
            260                 265                 270         


Asp Asn Ala Lys Thr Ile Ile Val His Leu Asn Gln Thr Val Glu Ile 
        275                 280                 285             


Val Cys Ile Arg Pro Asn Asn Asn Thr Arg Gln Ser Ile Arg Ile Gly 
    290                 295                 300                 


Pro Gly Gln Val Phe Tyr Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg 
305                 310                 315                 320 


Gln Ala Tyr Cys Thr Ile Asn Thr Thr Ala Trp Asn Glu Thr Leu Gln 
                325                 330                 335     


Arg Val Ser Lys Lys Leu Ala Glu His Phe Pro Asn Lys Thr Ile Arg 
            340                 345                 350         


Phe Ala Pro Ser Ser Gly Gly Asp Leu Glu Ile Thr Thr His Ser Phe 
        355                 360                 365             


Asn Cys Arg Gly Glu Phe Phe Tyr Cys Asn Thr Ser Gly Leu Phe Asn 
    370                 375                 380                 


Ser Thr Tyr Met Thr Asn Gly Thr Phe Thr Tyr Lys Leu Asn Asp Thr 
385                 390                 395                 400 


Asn Ile Thr Ile Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln 
                405                 410                 415     


Glu Val Gly Arg Cys Met Tyr Ala Pro Pro Ile Ala Gly Asn Ile Thr 
            420                 425                 430         


Cys Lys Ser Asn Ile Thr Gly Met Leu Leu Val Arg Asp Gly Gly Lys 
        435                 440                 445             


Asn Glu Asn Ser Thr Glu Glu Thr Phe Arg Pro Gly Gly Gly Asn Met 
    450                 455                 460                 


Arg Asp Asn Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Glu Ile 
465                 470                 475                 480 


Lys Pro Leu Gly Val Ala Pro Thr Lys Cys Lys Arg Arg Val Val Gly 
                485                 490                 495     


Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly 
            500                 505                 510         


Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu 
        515                 520                 525             


Thr Val Gln Ala Arg Asn Leu Leu Ser Gly Ile Val Gln Gln Gln Ser 
    530                 535                 540                 


Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Leu Leu Lys Leu Thr 
545                 550                 555                 560 


Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg 
                565                 570                 575     


Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys 
            580                 585                 590         


Leu Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Arg 
        595                 600                 605             


Asn Leu Ser Glu Ile Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys 
    610                 615                 620                 


Glu Ile Ser Asn Tyr Thr Gln Ile Ile Tyr Gly Leu Leu Glu Glu Ser 
625                 630                 635                 640 


Gln Asn Gln Gln Glu Lys Asn Glu Gln Asp Leu Leu Ala Leu 
                645                 650                 


<210>  38
<211>  675
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant HIV-1 Env sequence

<400>  38

Met Asp Ser Lys Gly Ser Ser Gln Lys Gly Ser Arg Leu Leu Leu Leu 
1               5                   10                  15      


Leu Val Val Ser Asn Leu Leu Leu Pro Gln Gly Val Leu Ala Ala Glu 
            20                  25                  30          


Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val Trp Lys Asp Ala 
        35                  40                  45              


Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala Tyr Glu Thr Glu 
    50                  55                  60                  


Lys His Asn Val Trp Ala Thr His Ala Cys Val Pro Thr Asp Pro Asn 
65                  70                  75                  80  


Pro Gln Glu Ile His Leu Glu Asn Val Thr Glu Glu Phe Asn Met Trp 
                85                  90                  95      


Lys Asn Asn Met Val Glu Gln Met His Thr Asp Ile Ile Ser Leu Trp 
            100                 105                 110         


Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro Leu Cys Val Thr 
        115                 120                 125             


Leu Gln Cys Thr Asn Val Thr Asn Asn Ile Thr Asp Asp Met Arg Gly 
    130                 135                 140                 


Glu Leu Lys Asn Cys Ser Phe Asn Met Thr Thr Glu Leu Arg Asp Lys 
145                 150                 155                 160 


Lys Gln Lys Val Tyr Ser Leu Phe Tyr Arg Leu Asp Val Val Gln Ile 
                165                 170                 175     


Asn Glu Asn Gln Gly Asn Arg Ser Asn Asn Ser Asn Lys Glu Tyr Arg 
            180                 185                 190         


Leu Ile Asn Cys Asn Thr Ser Ala Cys Thr Gln Ala Cys Pro Lys Val 
        195                 200                 205             


Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro Ala Gly Phe Ala 
    210                 215                 220                 


Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr Gly Pro Cys Pro 
225                 230                 235                 240 


Ser Val Ser Thr Val Gln Cys Thr His Gly Ile Lys Pro Val Val Ser 
                245                 250                 255     


Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu Glu Val Met Ile 
            260                 265                 270         


Arg Ser Glu Asn Ile Thr Asn Asn Ala Lys Asn Ile Leu Val Gln Phe 
        275                 280                 285             


Asn Thr Pro Val Gln Ile Asn Cys Thr Arg Pro Asn Asn Asn Thr Arg 
    290                 295                 300                 


Lys Ser Ile Arg Ile Gly Pro Gly Gln Ala Phe Tyr Ala Thr Gly Asp 
305                 310                 315                 320 


Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Val Ser Lys Ala Thr 
                325                 330                 335     


Trp Asn Glu Thr Leu Gly Lys Val Val Lys Gln Leu Arg Lys His Phe 
            340                 345                 350         


Gly Asn Asn Thr Ile Ile Arg Phe Ala Asn Ser Ser Gly Gly Asp Leu 
        355                 360                 365             


Glu Val Thr Thr His Ser Phe Asn Cys Gly Gly Glu Phe Phe Tyr Cys 
    370                 375                 380                 


Asn Thr Ser Gly Leu Phe Asn Ser Thr Trp Ile Ser Asn Thr Ser Val 
385                 390                 395                 400 


Gln Gly Ser Asn Ser Thr Gly Ser Asn Asp Ser Ile Thr Leu Pro Cys 
                405                 410                 415     


Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Ile Gly Gln Cys Met 
            420                 425                 430         


Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Val Ser Asn Ile Thr 
        435                 440                 445             


Gly Leu Ile Leu Thr Arg Asp Gly Gly Ser Thr Asn Ser Thr Thr Glu 
    450                 455                 460                 


Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn Trp Arg Ser Glu 
465                 470                 475                 480 


Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu Gly Val Ala Pro 
                485                 490                 495     


Thr Arg Cys Lys Arg Arg Val Val Gly Asn Ser Thr His Lys Gln Leu 
            500                 505                 510         


Thr His His Met Arg Arg Arg Arg Arg Arg Ala Val Gly Ile Gly Ala 
        515                 520                 525             


Val Phe Leu Gly Phe Leu Gly Ala Ala Gly Ser Thr Met Gly Ala Ala 
    530                 535                 540                 


Ser Met Thr Leu Thr Val Gln Ala Arg Asn Leu Leu Ser Gly Ile Val 
545                 550                 555                 560 


Gln Gln Gln Ser Asn Leu Leu Arg Ala Pro Glu Ala Gln Gln His Leu 
                565                 570                 575     


Leu Lys Leu Thr Val Trp Gly Ile Lys Gln Leu Gln Ala Arg Val Leu 
            580                 585                 590         


Ala Val Glu Arg Tyr Leu Arg Asp Gln Gln Leu Leu Gly Ile Trp Gly 
        595                 600                 605             


Cys Ser Gly Lys Leu Ile Cys Cys Thr Asn Val Pro Trp Asn Ser Ser 
    610                 615                 620                 


Trp Ser Asn Arg Asn Leu Ser Glu Ile Trp Asp Asn Met Thr Trp Leu 
625                 630                 635                 640 


Gln Trp Asp Lys Glu Ile Ser Asn Tyr Thr Gln Ile Ile Tyr Gly Leu 
                645                 650                 655     


Leu Glu Glu Ser Gln Asn Gln Gln Glu Lys Asn Glu Gln Asp Leu Leu 
            660                 665                 670         


Ala Leu Asp 
        675 


