                         SEQUENCE LISTING

<110>  The University of North Carolina at Chapel Hill
       Lakshmanane, Premkumar
       Desilva, Aravinda
 
<120>  METHODS AND COMPOSITIONS FOR ZIKA VIRUS DETECTION

<130>  5470-822WO

<150>  US 62/572,908
<151>  2017-10-16

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  560
<212>  PRT
<213>  Artificial

<220>
<223>  Zika EDI MBP

<400>  1

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Lys Ser Ser His His His 
            20                  25                  30          


His His His Gly Ser Ser Met Lys Ile Glu Glu Gly Lys Leu Val Ile 
        35                  40                  45              


Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys 
    50                  55                  60                  


Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu His Pro Asp 
65                  70                  75                  80  


Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly Asp Gly Pro 
                85                  90                  95      


Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr Ala Gln Ser 
            100                 105                 110         


Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu 
        115                 120                 125             


Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala 
    130                 135                 140                 


Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu 
145                 150                 155                 160 


Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys 
                165                 170                 175     


Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn Leu Gln Glu 
            180                 185                 190         


Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe 
        195                 200                 205             


Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly Val Asp Asn 
    210                 215                 220                 


Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu Ile Lys Asn 
225                 230                 235                 240 


Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe 
                245                 250                 255     


Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp Ala Trp Ser 
            260                 265                 270         


Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val Leu Pro Thr 
        275                 280                 285             


Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu Ser Ala Gly 
    290                 295                 300                 


Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu 
305                 310                 315                 320 


Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn Lys Asp Lys 
                325                 330                 335     


Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys 
            340                 345                 350         


Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys Gly Glu Ile 
        355                 360                 365             


Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala Val Arg Thr 
    370                 375                 380                 


Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp Glu Ala Leu 
385                 390                 395                 400 


Lys Asp Ala Gln Thr Asn Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn 
                405                 410                 415     


Asn Asn Asn Leu Gly Ile Glu Glu Asn Leu Tyr Phe Gln Ser Asn Ala 
            420                 425                 430         


Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
        435                 440                 445             


Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 
    450                 455                 460                 


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
465                 470                 475                 480 


Thr Asn Gly Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His 
                485                 490                 495     


Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg 
            500                 505                 510         


Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 
        515                 520                 525             


Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Ser 
    530                 535                 540                 


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
545                 550                 555                 560 


<210>  2
<211>  534
<212>  PRT
<213>  Artificial

<220>
<223>  Zika EDIII MBP

<400>  2

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Lys Ser Ser His His His 
            20                  25                  30          


His His His Gly Ser Ser Met Lys Ile Glu Glu Gly Lys Leu Val Ile 
        35                  40                  45              


Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys 
    50                  55                  60                  


Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu His Pro Asp 
65                  70                  75                  80  


Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly Asp Gly Pro 
                85                  90                  95      


Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr Ala Gln Ser 
            100                 105                 110         


Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu 
        115                 120                 125             


Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala 
    130                 135                 140                 


Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu 
145                 150                 155                 160 


Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys 
                165                 170                 175     


Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn Leu Gln Glu 
            180                 185                 190         


Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe 
        195                 200                 205             


Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly Val Asp Asn 
    210                 215                 220                 


Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu Ile Lys Asn 
225                 230                 235                 240 


Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe 
                245                 250                 255     


Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp Ala Trp Ser 
            260                 265                 270         


Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val Leu Pro Thr 
        275                 280                 285             


Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu Ser Ala Gly 
    290                 295                 300                 


Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu 
305                 310                 315                 320 


Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn Lys Asp Lys 
                325                 330                 335     


Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys 
            340                 345                 350         


Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys Gly Glu Ile 
        355                 360                 365             


Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala Val Arg Thr 
    370                 375                 380                 


Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp Glu Ala Leu 
385                 390                 395                 400 


Lys Asp Ala Gln Thr Asn Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn 
                405                 410                 415     


Asn Asn Asn Leu Gly Ile Glu Glu Asn Leu Tyr Phe Gln Ser Asn Ala 
            420                 425                 430         


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
        435                 440                 445             


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
    450                 455                 460                 


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
465                 470                 475                 480 


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
                485                 490                 495     


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
            500                 505                 510         


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
        515                 520                 525             


His His Trp His Arg Ser 
    530                 


<210>  3
<211>  534
<212>  PRT
<213>  Artificial

<220>
<223>  Zika EDIII variant MBP

<400>  3

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Lys Ser Ser His His His 
            20                  25                  30          


His His His Gly Ser Ser Met Lys Ile Glu Glu Gly Lys Leu Val Ile 
        35                  40                  45              


Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys 
    50                  55                  60                  


Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu His Pro Asp 
65                  70                  75                  80  


Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly Asp Gly Pro 
                85                  90                  95      


Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr Ala Gln Ser 
            100                 105                 110         


Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu 
        115                 120                 125             


Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala 
    130                 135                 140                 


Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu 
145                 150                 155                 160 


Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys 
                165                 170                 175     


Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn Leu Gln Glu 
            180                 185                 190         


Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe 
        195                 200                 205             


Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly Val Asp Asn 
    210                 215                 220                 


Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu Ile Lys Asn 
225                 230                 235                 240 


Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe 
                245                 250                 255     


Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp Ala Trp Ser 
            260                 265                 270         


Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val Leu Pro Thr 
        275                 280                 285             


Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu Ser Ala Gly 
    290                 295                 300                 


Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu 
305                 310                 315                 320 


Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn Lys Asp Lys 
                325                 330                 335     


Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys 
            340                 345                 350         


Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys Gly Glu Ile 
        355                 360                 365             


Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala Val Arg Thr 
    370                 375                 380                 


Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp Glu Ala Leu 
385                 390                 395                 400 


Lys Asp Ala Gln Thr Asn Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn 
                405                 410                 415     


Asn Asn Asn Leu Gly Ile Glu Glu Asn Leu Tyr Phe Gln Ser Asn Ala 
            420                 425                 430         


Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys His 
        435                 440                 445             


Pro Ala Glu Thr Gly His Gly Thr Val Gln Val Glu Val Gln Tyr Ala 
    450                 455                 460                 


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Thr Asp Leu 
465                 470                 475                 480 


Asn Asp Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
                485                 490                 495     


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
            500                 505                 510         


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
        515                 520                 525             


His His Trp His Arg Ser 
    530                 


<210>  4
<211>  436
<212>  PRT
<213>  Artificial

<220>
<223>  Zika EDIII Halo-tag

<400>  4

Met Ala Glu Ile Gly Thr Gly Phe Pro Phe Asp Pro His Tyr Val Glu 
1               5                   10                  15      


Val Leu Gly Glu Arg Met His Tyr Val Asp Val Gly Pro Arg Asp Gly 
            20                  25                  30          


Thr Pro Val Leu Phe Leu His Gly Asn Pro Thr Ser Ser Tyr Val Trp 
        35                  40                  45              


Arg Asn Ile Ile Pro His Val Ala Pro Thr His Arg Cys Ile Ala Pro 
    50                  55                  60                  


Asp Leu Ile Gly Met Gly Lys Ser Asp Lys Pro Asp Leu Gly Tyr Phe 
65                  70                  75                  80  


Phe Asp Asp His Val Arg Phe Met Asp Ala Phe Ile Glu Ala Leu Gly 
                85                  90                  95      


Leu Glu Glu Val Val Leu Val Ile His Asp Trp Gly Ser Ala Leu Gly 
            100                 105                 110         


Phe His Trp Ala Lys Arg Asn Pro Glu Arg Val Lys Gly Ile Ala Phe 
        115                 120                 125             


Met Glu Phe Ile Arg Pro Ile Pro Thr Trp Asp Glu Trp Pro Glu Phe 
    130                 135                 140                 


Ala Arg Glu Thr Phe Gln Ala Phe Arg Thr Thr Asp Val Gly Arg Lys 
145                 150                 155                 160 


Leu Ile Ile Asp Gln Asn Val Phe Ile Glu Gly Thr Leu Pro Met Gly 
                165                 170                 175     


Val Val Arg Pro Leu Thr Glu Val Glu Met Asp His Tyr Arg Glu Pro 
            180                 185                 190         


Phe Leu Asn Pro Val Asp Arg Glu Pro Leu Trp Arg Phe Pro Asn Glu 
        195                 200                 205             


Leu Pro Ile Ala Gly Glu Pro Ala Asn Ile Val Ala Leu Val Glu Glu 
    210                 215                 220                 


Tyr Met Asp Trp Leu His Gln Ser Pro Val Pro Lys Leu Leu Phe Trp 
225                 230                 235                 240 


Gly Thr Pro Gly Val Leu Ile Pro Pro Ala Glu Ala Ala Arg Leu Ala 
                245                 250                 255     


Lys Ser Leu Pro Asn Cys Lys Ala Val Asp Ile Gly Pro Gly Leu Asn 
            260                 265                 270         


Leu Leu Gln Glu Asp Asn Pro Asp Leu Ile Gly Ser Glu Ile Ala Arg 
        275                 280                 285             


Trp Leu Ser Thr Leu Glu Ile Ser Gly Gly Gly Gly Gly Ser Gly Gly 
    290                 295                 300                 


Gly Ile Glu Glu Asn Leu Tyr Phe Gln Ser Asn Ala Gly Val Ser Tyr 
305                 310                 315                 320 


Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile Pro Ala Glu Thr 
                325                 330                 335     


Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala Gly Thr Asp Gly 
            340                 345                 350         


Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met Gln Thr Leu Thr 
        355                 360                 365             


Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile Thr Glu Ser Thr 
    370                 375                 380                 


Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro Phe Gly Asp Ser 
385                 390                 395                 400 


Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr His His Trp His 
                405                 410                 415     


Arg Ser Gly Ser Ser Gly Gly Ser Leu Pro Glu Thr Gly Gly His His 
            420                 425                 430         


His His His His 
        435     


<210>  5
<211>  360
<212>  PRT
<213>  Artificial

<220>
<223>  Zika NS1 C-terminal His

<400>  5

Asp Val Gly Cys Ser Val Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly 
1               5                   10                  15      


Thr Gly Val Phe Val Tyr Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr 
            20                  25                  30          


Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val Lys Gln 
        35                  40                  45              


Ala Trp Glu Asp Gly Ile Cys Gly Ile Ser Ser Val Ser Arg Met Glu 
    50                  55                  60                  


Asn Ile Met Trp Arg Ser Val Glu Gly Glu Leu Asn Ala Ile Leu Glu 
65                  70                  75                  80  


Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser Val Lys Asn Pro 
                85                  90                  95      


Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val Asn Glu Leu Pro 
            100                 105                 110         


His Gly Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys 
        115                 120                 125             


Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu Cys Pro 
    130                 135                 140                 


Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His Gly Phe 
145                 150                 155                 160 


Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg Glu Asp Tyr Ser 
                165                 170                 175     


Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly Lys Glu 
            180                 185                 190         


Ala Val His Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp 
        195                 200                 205             


Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr Cys Glu 
    210                 215                 220                 


Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Ile Glu Glu Ser Asp 
225                 230                 235                 240 


Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr 
                245                 250                 255     


Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly Pro Trp His Ser Glu Glu 
            260                 265                 270         


Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His Val Glu 
        275                 280                 285             


Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser 
    290                 295                 300                 


Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro Pro 
305                 310                 315                 320 


Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg 
                325                 330                 335     


Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser Met Val Thr Ala 
            340                 345                 350         


Gly Gly His His His His His His 
        355                 360 


<210>  6
<211>  356
<212>  PRT
<213>  Artificial

<220>
<223>  Zika NS1 N-terminal His

<400>  6

Gly His His His His His His Asp Val Gly Cys Ser Val Asp Phe Ser 
1               5                   10                  15      


Lys Lys Glu Thr Arg Cys Gly Thr Gly Val Phe Val Tyr Asn Asp Val 
            20                  25                  30          


Glu Ala Trp Arg Asp Arg Tyr Lys Tyr His Pro Asp Ser Pro Arg Arg 
        35                  40                  45              


Leu Ala Ala Ala Val Lys Gln Ala Trp Glu Asp Gly Ile Cys Ser Ser 
    50                  55                  60                  


Val Ser Arg Met Asn Ile Met Trp Arg Ser Val Glu Gly Glu Leu Asn 
65                  70                  75                  80  


Ala Ile Leu Glu Glu Asn Gly Val Gln Leu Thr Val Val Val Gly Ser 
                85                  90                  95      


Val Lys Asn Pro Met Trp Arg Gly Pro Gln Arg Leu Pro Val Pro Val 
            100                 105                 110         


Asn Glu Leu Pro His Gly Trp Lys Ala Trp Gly Lys Ser Tyr Phe Val 
        115                 120                 125             


Arg Ala Ala Lys Thr Asn Asn Ser Phe Val Val Asp Gly Asp Thr Leu 
    130                 135                 140                 


Lys Glu Cys Pro Leu Lys His Arg Ala Trp Asn Ser Phe Leu Val Glu 
145                 150                 155                 160 


Asp His Gly Phe Gly Val Phe His Thr Ser Val Trp Leu Lys Val Arg 
                165                 170                 175     


Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala Val 
            180                 185                 190         


Lys Gly Lys Glu Ala Val His Ser Asp Leu Gly Tyr Trp Ile Glu Ser 
        195                 200                 205             


Glu Lys Asn Asp Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu Met 
    210                 215                 220                 


Lys Thr Cys Glu Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly Ile 
225                 230                 235                 240 


Glu Glu Ser Asp Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu Ser 
                245                 250                 255     


His His Asn Thr Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly Pro Trp 
            260                 265                 270         


His Ser Glu Glu Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr Lys 
        275                 280                 285             


Val His Val Glu Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg Ser 
    290                 295                 300                 


Thr Thr Ala Ser Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu Cys 
305                 310                 315                 320 


Thr Met Pro Pro Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr Gly 
                325                 330                 335     


Met Glu Ile Arg Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg Ser 
            340                 345                 350         


Met Val Thr Ala 
        355     


<210>  7
<211>  613
<212>  PRT
<213>  Artificial

<220>
<223>  Zika C-terminal NS1 MBP

<400>  7

Met Lys Ile Lys Thr Gly Ala Arg Ile Leu Ala Leu Ser Ala Leu Thr 
1               5                   10                  15      


Thr Met Met Phe Ser Ala Ser Ala Leu Ala Lys Ser Ser His His His 
            20                  25                  30          


His His His Gly Ser Ser Met Lys Ile Glu Glu Gly Lys Leu Val Ile 
        35                  40                  45              


Trp Ile Asn Gly Asp Lys Gly Tyr Asn Gly Leu Ala Glu Val Gly Lys 
    50                  55                  60                  


Lys Phe Glu Lys Asp Thr Gly Ile Lys Val Thr Val Glu His Pro Asp 
65                  70                  75                  80  


Lys Leu Glu Glu Lys Phe Pro Gln Val Ala Ala Thr Gly Asp Gly Pro 
                85                  90                  95      


Asp Ile Ile Phe Trp Ala His Asp Arg Phe Gly Gly Tyr Ala Gln Ser 
            100                 105                 110         


Gly Leu Leu Ala Glu Ile Thr Pro Asp Lys Ala Phe Gln Asp Lys Leu 
        115                 120                 125             


Tyr Pro Phe Thr Trp Asp Ala Val Arg Tyr Asn Gly Lys Leu Ile Ala 
    130                 135                 140                 


Tyr Pro Ile Ala Val Glu Ala Leu Ser Leu Ile Tyr Asn Lys Asp Leu 
145                 150                 155                 160 


Leu Pro Asn Pro Pro Lys Thr Trp Glu Glu Ile Pro Ala Leu Asp Lys 
                165                 170                 175     


Glu Leu Lys Ala Lys Gly Lys Ser Ala Leu Met Phe Asn Leu Gln Glu 
            180                 185                 190         


Pro Tyr Phe Thr Trp Pro Leu Ile Ala Ala Asp Gly Gly Tyr Ala Phe 
        195                 200                 205             


Lys Tyr Glu Asn Gly Lys Tyr Asp Ile Lys Asp Val Gly Val Asp Asn 
    210                 215                 220                 


Ala Gly Ala Lys Ala Gly Leu Thr Phe Leu Val Asp Leu Ile Lys Asn 
225                 230                 235                 240 


Lys His Met Asn Ala Asp Thr Asp Tyr Ser Ile Ala Glu Ala Ala Phe 
                245                 250                 255     


Asn Lys Gly Glu Thr Ala Met Thr Ile Asn Gly Pro Trp Ala Trp Ser 
            260                 265                 270         


Asn Ile Asp Thr Ser Lys Val Asn Tyr Gly Val Thr Val Leu Pro Thr 
        275                 280                 285             


Phe Lys Gly Gln Pro Ser Lys Pro Phe Val Gly Val Leu Ser Ala Gly 
    290                 295                 300                 


Ile Asn Ala Ala Ser Pro Asn Lys Glu Leu Ala Lys Glu Phe Leu Glu 
305                 310                 315                 320 


Asn Tyr Leu Leu Thr Asp Glu Gly Leu Glu Ala Val Asn Lys Asp Lys 
                325                 330                 335     


Pro Leu Gly Ala Val Ala Leu Lys Ser Tyr Glu Glu Glu Leu Ala Lys 
            340                 345                 350         


Asp Pro Arg Ile Ala Ala Thr Met Glu Asn Ala Gln Lys Gly Glu Ile 
        355                 360                 365             


Met Pro Asn Ile Pro Gln Met Ser Ala Phe Trp Tyr Ala Val Arg Thr 
    370                 375                 380                 


Ala Val Ile Asn Ala Ala Ser Gly Arg Gln Thr Val Asp Glu Ala Leu 
385                 390                 395                 400 


Lys Asp Ala Gln Thr Asn Ser Ser Ser Asn Asn Asn Asn Asn Asn Asn 
                405                 410                 415     


Asn Asn Asn Leu Gly Ile Glu Glu Asn Leu Tyr Phe Gln Ser Asn Ala 
            420                 425                 430         


Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala 
        435                 440                 445             


Val Lys Gly Lys Glu Ala Val His Ser Asp Leu Gly Tyr Trp Ile Glu 
    450                 455                 460                 


Ser Glu Lys Asn Asp Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu 
465                 470                 475                 480 


Met Lys Thr Cys Glu Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly 
                485                 490                 495     


Ile Glu Glu Ser Asp Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu 
            500                 505                 510         


Ser His His Asn Thr Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly Pro 
        515                 520                 525             


Trp His Ser Glu Glu Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr 
    530                 535                 540                 


Lys Val His Val Glu Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg 
545                 550                 555                 560 


Ser Thr Thr Ala Ser Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu 
                565                 570                 575     


Cys Thr Met Pro Pro Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr 
            580                 585                 590         


Gly Met Glu Ile Arg Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg 
        595                 600                 605             


Ser Met Val Thr Ala 
    610             


<210>  8
<211>  102
<212>  PRT
<213>  Artificial

<220>
<223>  Zika EDIII domain

<400>  8

Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys Ile 
1               5                   10                  15      


Pro Ala Glu Thr Leu His Gly Thr Val Thr Val Glu Val Gln Tyr Ala 
            20                  25                  30          


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Val Asp Met 
        35                  40                  45              


Gln Thr Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    50                  55                  60                  


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
65                  70                  75                  80  


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
                85                  90                  95      


His His Trp His Arg Ser 
            100         


<210>  9
<211>  128
<212>  PRT
<213>  Artificial

<220>
<223>  Zika EDI domain

<400>  9

Ile Arg Cys Ile Gly Val Ser Asn Arg Asp Phe Val Glu Gly Met Ser 
1               5                   10                  15      


Gly Gly Thr Trp Val Asp Val Val Leu Glu His Gly Gly Cys Val Thr 
            20                  25                  30          


Val Met Ala Gln Asp Lys Pro Thr Val Asp Ile Glu Leu Val Thr Thr 
        35                  40                  45              


Thr Asn Gly Glu Tyr Arg Ile Met Leu Ser Val His Gly Ser Gln His 
    50                  55                  60                  


Ser Gly Met Ile Val Asn Asp Thr Gly His Glu Thr Asp Glu Asn Arg 
65                  70                  75                  80  


Ala Lys Val Glu Ile Thr Pro Asn Ser Pro Arg Ala Glu Ala Thr Leu 
                85                  90                  95      


Gly Gly Phe Gly Ser Leu Gly Leu Asp Cys Glu Pro Arg Thr Gly Ser 
            100                 105                 110         


Gly His Leu Lys Cys Arg Leu Lys Met Asp Lys Leu Arg Leu Lys Gly 
        115                 120                 125             


<210>  10
<211>  102
<212>  PRT
<213>  Artificial

<220>
<223>  Zika EDIII domain variant

<400>  10

Gly Val Ser Tyr Ser Leu Cys Thr Ala Ala Phe Thr Phe Thr Lys His 
1               5                   10                  15      


Pro Ala Glu Thr Gly His Gly Thr Val Gln Val Glu Val Gln Tyr Ala 
            20                  25                  30          


Gly Thr Asp Gly Pro Cys Lys Val Pro Ala Gln Met Ala Thr Asp Leu 
        35                  40                  45              


Asn Asp Leu Thr Pro Val Gly Arg Leu Ile Thr Ala Asn Pro Val Ile 
    50                  55                  60                  


Thr Glu Ser Thr Glu Asn Ser Lys Met Met Leu Glu Leu Asp Pro Pro 
65                  70                  75                  80  


Phe Gly Asp Ser Tyr Ile Val Ile Gly Val Gly Glu Lys Lys Ile Thr 
                85                  90                  95      


His His Trp His Arg Ser 
            100         


<210>  11
<211>  181
<212>  PRT
<213>  Artificial

<220>
<223>  Zika NS1 C-terminal beta ladder domain

<400>  11

Arg Glu Asp Tyr Ser Leu Glu Cys Asp Pro Ala Val Ile Gly Thr Ala 
1               5                   10                  15      


Val Lys Gly Lys Glu Ala Val His Ser Asp Leu Gly Tyr Trp Ile Glu 
            20                  25                  30          


Ser Glu Lys Asn Asp Thr Trp Arg Leu Lys Arg Ala His Leu Ile Glu 
        35                  40                  45              


Met Lys Thr Cys Glu Trp Pro Lys Ser His Thr Leu Trp Thr Asp Gly 
    50                  55                  60                  


Ile Glu Glu Ser Asp Leu Ile Ile Pro Lys Ser Leu Ala Gly Pro Leu 
65                  70                  75                  80  


Ser His His Asn Thr Arg Glu Gly Tyr Arg Thr Gln Met Lys Gly Pro 
                85                  90                  95      


Trp His Ser Glu Glu Leu Glu Ile Arg Phe Glu Glu Cys Pro Gly Thr 
            100                 105                 110         


Lys Val His Val Glu Glu Thr Cys Gly Thr Arg Gly Pro Ser Leu Arg 
        115                 120                 125             


Ser Thr Thr Ala Ser Gly Arg Val Ile Glu Glu Trp Cys Cys Arg Glu 
    130                 135                 140                 


Cys Thr Met Pro Pro Leu Ser Phe Arg Ala Lys Asp Gly Cys Trp Tyr 
145                 150                 155                 160 


Gly Met Glu Ile Arg Pro Arg Lys Glu Pro Glu Ser Asn Leu Val Arg 
                165                 170                 175     


Ser Met Val Thr Ala 
            180     


<210>  12
<211>  349
<212>  PRT
<213>  Zika NS1 polypeptide

<400>  12

Asp Val Gly Cys Ser Val Asp Phe Ser Lys Lys Glu Thr Arg Cys Gly 
1               5                   10                  15      


Thr Gly Val Phe Val Tyr Asn Asp Val Glu Ala Trp Arg Asp Arg Tyr 
            20                  25                  30          


Lys Tyr His Pro Asp Ser Pro Arg Arg Leu Ala Ala Ala Val Lys Gln 
        35                  40                  45              


Ala Trp Glu Asp Gly Ile Cys Ser Ser Val Ser Arg Met Asn Ile Met 
    50                  55                  60                  


Trp Arg Ser Val Glu Gly Glu Leu Asn Ala Ile Leu Glu Glu Asn Gly 
65                  70                  75                  80  


Val Gln Leu Thr Val Val Val Gly Ser Val Lys Asn Pro Met Trp Arg 
                85                  90                  95      


Gly Pro Gln Arg Leu Pro Val Pro Val Asn Glu Leu Pro His Gly Trp 
            100                 105                 110         


Lys Ala Trp Gly Lys Ser Tyr Phe Val Arg Ala Ala Lys Thr Asn Asn 
        115                 120                 125             


Ser Phe Val Val Asp Gly Asp Thr Leu Lys Glu Cys Pro Leu Lys His 
    130                 135                 140                 


Arg Ala Trp Asn Ser Phe Leu Val Glu Asp His Gly Phe Gly Val Phe 
145                 150                 155                 160 


His Thr Ser Val Trp Leu Lys Val Arg Glu Asp Tyr Ser Leu Glu Cys 
                165                 170                 175     


Asp Pro Ala Val Ile Gly Thr Ala Val Lys Gly Lys Glu Ala Val His 
            180                 185                 190         


Ser Asp Leu Gly Tyr Trp Ile Glu Ser Glu Lys Asn Asp Thr Trp Arg 
        195                 200                 205             


Leu Lys Arg Ala His Leu Ile Glu Met Lys Thr Cys Glu Trp Pro Lys 
    210                 215                 220                 


Ser His Thr Leu Trp Thr Asp Gly Ile Glu Glu Ser Asp Leu Ile Ile 
225                 230                 235                 240 


Pro Lys Ser Leu Ala Gly Pro Leu Ser His His Asn Thr Arg Glu Gly 
                245                 250                 255     


Tyr Arg Thr Gln Met Lys Gly Pro Trp His Ser Glu Glu Leu Glu Ile 
            260                 265                 270         


Arg Phe Glu Glu Cys Pro Gly Thr Lys Val His Val Glu Glu Thr Cys 
        275                 280                 285             


Gly Thr Arg Gly Pro Ser Leu Arg Ser Thr Thr Ala Ser Gly Arg Val 
    290                 295                 300                 


Ile Glu Glu Trp Cys Cys Arg Glu Cys Thr Met Pro Pro Leu Ser Phe 
305                 310                 315                 320 


Arg Ala Lys Asp Gly Cys Trp Tyr Gly Met Glu Ile Arg Pro Arg Lys 
                325                 330                 335     


Glu Pro Glu Ser Asn Leu Val Arg Ser Met Val Thr Ala 
            340                 345                 


