                         SEQUENCE LISTING

<110>  Elanco US Inc.
       Ambrx Inc.
 
<120>  PORCINE G-CSF VARIANTS AND THEIR USES

<130>  X21439_WO

<150>  US 62/570,877
<151>  2017-10-11

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  175
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial

<400>  1

Met Ala Pro Leu Ser Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu 
            20                  25                  30          


Gln Glu Arg Leu Cys Ala Thr His Lys Leu Cys His Pro Gln Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Leu Pro Gln Ala Ser Leu Ser Ser 
    50                  55                  60                  


Cys Ser Ser Gln Ala Leu Gln Leu Thr Gly Cys Leu Asn Gln Leu His 
65                  70                  75                  80  


Gly Gly Leu Val Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Ala Pro Ala Leu Asp Ile Leu Gln Leu Asp Val Thr 
            100                 105                 110         


Asp Leu Ala Thr Asn Ile Trp Leu Gln Met Glu Asp Leu Arg Met Ala 
        115                 120                 125             


Pro Ala Ser Leu Pro Thr Gln Gly Thr Val Pro Thr Phe Thr Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Val Ser Gln Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Leu Ala Tyr Arg Val Leu Arg Tyr Leu Ala Glu Pro 
                165                 170                 175 


<210>  2
<211>  175
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial


<220>
<221>  MISC_FEATURE
<222>  (44)..(44)
<223>  Xaa = pAF

<400>  2

Met Ala Pro Leu Ser Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu 
            20                  25                  30          


Gln Glu Arg Leu Cys Ala Thr His Lys Leu Cys Xaa Pro Gln Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Leu Pro Gln Ala Ser Leu Ser Ser 
    50                  55                  60                  


Cys Ser Ser Gln Ala Leu Gln Leu Thr Gly Cys Leu Asn Gln Leu His 
65                  70                  75                  80  


Gly Gly Leu Val Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Ala Pro Ala Leu Asp Ile Leu Gln Leu Asp Val Thr 
            100                 105                 110         


Asp Leu Ala Thr Asn Ile Trp Leu Gln Met Glu Asp Leu Arg Met Ala 
        115                 120                 125             


Pro Ala Ser Leu Pro Thr Gln Gly Thr Val Pro Thr Phe Thr Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Val Ser Gln Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Leu Ala Tyr Arg Val Leu Arg Tyr Leu Ala Glu Pro 
                165                 170                 175 


<210>  3
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Natural

<400>  3

Ala Pro Leu Ser Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys 
1               5                   10                  15      


Cys Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu Gln 
            20                  25                  30          


Glu Arg Leu Cys Ala Thr His Lys Leu Cys His Pro Gln Glu Leu Val 
        35                  40                  45              


Leu Leu Gly His Ser Leu Gly Leu Pro Gln Ala Ser Leu Ser Ser Cys 
    50                  55                  60                  


Ser Ser Gln Ala Leu Gln Leu Thr Gly Cys Leu Asn Gln Leu His Gly 
65                  70                  75                  80  


Gly Leu Val Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile Ser 
                85                  90                  95      


Pro Glu Leu Ala Pro Ala Leu Asp Ile Leu Gln Leu Asp Val Thr Asp 
            100                 105                 110         


Leu Ala Thr Asn Ile Trp Leu Gln Met Glu Asp Leu Arg Met Ala Pro 
        115                 120                 125             


Ala Ser Leu Pro Thr Gln Gly Thr Val Pro Thr Phe Thr Ser Ala Phe 
    130                 135                 140                 


Gln Arg Arg Ala Gly Gly Val Leu Val Val Ser Gln Leu Gln Ser Phe 
145                 150                 155                 160 


Leu Glu Leu Ala Tyr Arg Val Leu Arg Tyr Leu Ala Glu Pro 
                165                 170                 


<210>  4
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial


<220>
<221>  MISC_FEATURE
<222>  (43)..(43)
<223>  Xaa = pAF

<400>  4

Ala Pro Leu Ser Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys 
1               5                   10                  15      


Cys Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu Gln 
            20                  25                  30          


Glu Arg Leu Cys Ala Thr His Lys Leu Cys Xaa Pro Gln Glu Leu Val 
        35                  40                  45              


Leu Leu Gly His Ser Leu Gly Leu Pro Gln Ala Ser Leu Ser Ser Cys 
    50                  55                  60                  


Ser Ser Gln Ala Leu Gln Leu Thr Gly Cys Leu Asn Gln Leu His Gly 
65                  70                  75                  80  


Gly Leu Val Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile Ser 
                85                  90                  95      


Pro Glu Leu Ala Pro Ala Leu Asp Ile Leu Gln Leu Asp Val Thr Asp 
            100                 105                 110         


Leu Ala Thr Asn Ile Trp Leu Gln Met Glu Asp Leu Arg Met Ala Pro 
        115                 120                 125             


Ala Ser Leu Pro Thr Gln Gly Thr Val Pro Thr Phe Thr Ser Ala Phe 
    130                 135                 140                 


Gln Arg Arg Ala Gly Gly Val Leu Val Val Ser Gln Leu Gln Ser Phe 
145                 150                 155                 160 


Leu Glu Leu Ala Tyr Arg Val Leu Arg Tyr Leu Ala Glu Pro 
                165                 170                 


<210>  5
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA (artificial) encodes SEQ ID NO: 1&3

<400>  5
atggcccctc tcagccctgc cagctccctg ccccagagct tcctgctcaa gtgcttagag       60

caagtgagga aaatccaggc tgatggcgcc gagctgcagg agaggctgtg tgccacccac      120

aagctgtgcc acccccagga gctggtgctg ctcgggcact ctctgggcct cccccaggct      180

tccctgagca gctgctccag ccaggccctg cagctgactg gctgcctgaa ccaactgcat      240

ggcggcctcg tcctctacca gggcctcctg caggccctgg cgggcatctc cccagagctg      300

gcccccgccc tggacatact gcagctggat gtcaccgact tagccaccaa catctggctg      360

cagatggaag acctgaggat ggccccggcc tcgcttccca cccagggcac cgtgccgacc      420

ttcacctcgg ccttccagcg ccgggcagga ggggtcctgg ttgtctccca gctgcagagc      480

ttcctggagc tggcgtaccg tgtcctgcgc tacctcgccg agccctga                   528


<210>  6
<211>  528
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA (artificial) encodes SEQ ID NO: 2&4

<400>  6
atggcccctc tcagccctgc cagctccctg ccccagagct tcctgctcaa gtgcttagag       60

caagtgagga aaatccaggc tgatggcgcc gagctgcagg agaggctgtg tgccacccac      120

aagctgtgct agccccagga gctggtgctg ctcgggcact ctctgggcct cccccaggct      180

tccctgagca gctgctccag ccaggccctg cagctgactg gctgcctgaa ccaactgcat      240

ggcggcctcg tcctctacca gggcctcctg caggccctgg cgggcatctc cccagagctg      300

gcccccgccc tggacatact gcagctggat gtcaccgact tagccaccaa catctggctg      360

cagatggaag acctgaggat ggccccggcc tcgcttccca cccagggcac cgtgccgacc      420

ttcacctcgg ccttccagcg ccgggcagga ggggtcctgg ttgtctccca gctgcagagc      480

ttcctggagc tggcgtaccg tgtcctgcgc tacctcgccg agccctga                   528


<210>  7
<211>  175
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = norleucine or methionine

<220>
<221>  MISC_FEATURE
<222>  (44)..(44)
<223>  Xaa = pAF

<220>
<221>  MISC_FEATURE
<222>  (122)..(122)
<223>  Xaa = norleucine or methionine

<220>
<221>  MISC_FEATURE
<222>  (127)..(127)
<223>  Xaa = norleucine or methionine

<400>  7

Xaa Ala Pro Leu Ser Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Cys Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu 
            20                  25                  30          


Gln Glu Arg Leu Cys Ala Thr His Lys Leu Cys Xaa Pro Gln Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Leu Pro Gln Ala Ser Leu Ser Ser 
    50                  55                  60                  


Cys Ser Ser Gln Ala Leu Gln Leu Thr Gly Cys Leu Asn Gln Leu His 
65                  70                  75                  80  


Gly Gly Leu Val Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Ala Pro Ala Leu Asp Ile Leu Gln Leu Asp Val Thr 
            100                 105                 110         


Asp Leu Ala Thr Asn Ile Trp Leu Gln Xaa Glu Asp Leu Arg Xaa Ala 
        115                 120                 125             


Pro Ala Ser Leu Pro Thr Gln Gly Thr Val Pro Thr Phe Thr Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Val Ser Gln Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Leu Ala Tyr Arg Val Leu Arg Tyr Leu Ala Glu Pro 
                165                 170                 175 


<210>  8
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial


<220>
<221>  MISC_FEATURE
<222>  (42)..(42)
<223>  Xaa = pAF

<400>  8

Pro Leu Ser Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Cys 
1               5                   10                  15      


Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu Gln Glu 
            20                  25                  30          


Arg Leu Cys Ala Thr His Lys Leu Cys Xaa Pro Gln Glu Leu Val Leu 
        35                  40                  45              


Leu Gly His Ser Leu Gly Leu Pro Gln Ala Ser Leu Ser Ser Cys Ser 
    50                  55                  60                  


Ser Gln Ala Leu Gln Leu Thr Gly Cys Leu Asn Gln Leu His Gly Gly 
65                  70                  75                  80  


Leu Val Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile Ser Pro 
                85                  90                  95      


Glu Leu Ala Pro Ala Leu Asp Ile Leu Gln Leu Asp Val Thr Asp Leu 
            100                 105                 110         


Ala Thr Asn Ile Trp Leu Gln Met Glu Asp Leu Arg Met Ala Pro Ala 
        115                 120                 125             


Ser Leu Pro Thr Gln Gly Thr Val Pro Thr Phe Thr Ser Ala Phe Gln 
    130                 135                 140                 


Arg Arg Ala Gly Gly Val Leu Val Val Ser Gln Leu Gln Ser Phe Leu 
145                 150                 155                 160 


Glu Leu Ala Tyr Arg Val Leu Arg Tyr Leu Ala Glu Pro 
                165                 170             


<210>  9
<211>  175
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial


<220>
<221>  MISC_FEATURE
<222>  (44)..(44)
<223>  Xaa = pAF

<400>  9

Met Ala Pro Leu Ser Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu 
            20                  25                  30          


Gln Glu Arg Leu Cys Ala Thr His Lys Leu Cys Xaa Pro Gln Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Leu Pro Gln Ala Ser Leu Ser Ser 
    50                  55                  60                  


Cys Ser Ser Gln Ala Leu Gln Leu Thr Gly Cys Leu Asn Gln Leu His 
65                  70                  75                  80  


Gly Gly Leu Val Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Ala Pro Ala Leu Asp Ile Leu Gln Leu Asp Val Thr 
            100                 105                 110         


Asp Leu Ala Thr Asn Ile Trp Leu Gln Met Glu Asp Leu Arg Met Ala 
        115                 120                 125             


Pro Ala Ser Leu Pro Thr Gln Gly Thr Val Pro Thr Phe Thr Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Val Ser Gln Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Leu Ala Tyr Arg Val Leu Arg Tyr Leu Ala Glu Pro 
                165                 170                 175 


<210>  10
<211>  175
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = norleucine or methionine

<220>
<221>  MISC_FEATURE
<222>  (44)..(44)
<223>  Xaa = pAF

<220>
<221>  MISC_FEATURE
<222>  (122)..(122)
<223>  Xaa = norleucine or methionine

<220>
<221>  MISC_FEATURE
<222>  (127)..(127)
<223>  Xaa = norleucine or methionine

<400>  10

Xaa Ala Pro Leu Ser Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu 
1               5                   10                  15      


Lys Ser Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu 
            20                  25                  30          


Gln Glu Arg Leu Cys Ala Thr His Lys Leu Cys Xaa Pro Gln Glu Leu 
        35                  40                  45              


Val Leu Leu Gly His Ser Leu Gly Leu Pro Gln Ala Ser Leu Ser Ser 
    50                  55                  60                  


Cys Ser Ser Gln Ala Leu Gln Leu Thr Gly Cys Leu Asn Gln Leu His 
65                  70                  75                  80  


Gly Gly Leu Val Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile 
                85                  90                  95      


Ser Pro Glu Leu Ala Pro Ala Leu Asp Ile Leu Gln Leu Asp Val Thr 
            100                 105                 110         


Asp Leu Ala Thr Asn Ile Trp Leu Gln Xaa Glu Asp Leu Arg Xaa Ala 
        115                 120                 125             


Pro Ala Ser Leu Pro Thr Gln Gly Thr Val Pro Thr Phe Thr Ser Ala 
    130                 135                 140                 


Phe Gln Arg Arg Ala Gly Gly Val Leu Val Val Ser Gln Leu Gln Ser 
145                 150                 155                 160 


Phe Leu Glu Leu Ala Tyr Arg Val Leu Arg Tyr Leu Ala Glu Pro 
                165                 170                 175 


<210>  11
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial


<220>
<221>  MISC_FEATURE
<222>  (43)..(43)
<223>  Xaa = pAF

<400>  11

Ala Pro Leu Ser Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys 
1               5                   10                  15      


Cys Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu Gln 
            20                  25                  30          


Glu Arg Leu Cys Ala Thr His Lys Leu Cys Xaa Pro Gln Glu Leu Val 
        35                  40                  45              


Leu Leu Gly His Ser Leu Gly Leu Pro Gln Ala Ser Leu Ser Ser Cys 
    50                  55                  60                  


Ser Ser Gln Ala Leu Gln Leu Thr Gly Cys Leu Asn Gln Leu His Gly 
65                  70                  75                  80  


Gly Leu Val Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile Ser 
                85                  90                  95      


Pro Glu Leu Ala Pro Ala Leu Asp Ile Leu Gln Leu Asp Val Thr Asp 
            100                 105                 110         


Leu Ala Thr Asn Ile Trp Leu Gln Met Glu Asp Leu Arg Met Ala Pro 
        115                 120                 125             


Ala Ser Leu Pro Thr Gln Gly Thr Val Pro Thr Phe Thr Ser Ala Phe 
    130                 135                 140                 


Gln Arg Arg Ala Gly Gly Val Leu Val Val Ser Gln Leu Gln Ser Phe 
145                 150                 155                 160 


Leu Glu Leu Ala Tyr Arg Val Leu Arg Tyr Leu Ala Glu Pro 
                165                 170                 


<210>  12
<211>  173
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial


<220>
<221>  MISC_FEATURE
<222>  (42)..(42)
<223>  Xaa = pAF

<400>  12

Pro Leu Ser Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys Ser 
1               5                   10                  15      


Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu Gln Glu 
            20                  25                  30          


Arg Leu Cys Ala Thr His Lys Leu Cys Xaa Pro Gln Glu Leu Val Leu 
        35                  40                  45              


Leu Gly His Ser Leu Gly Leu Pro Gln Ala Ser Leu Ser Ser Cys Ser 
    50                  55                  60                  


Ser Gln Ala Leu Gln Leu Thr Gly Cys Leu Asn Gln Leu His Gly Gly 
65                  70                  75                  80  


Leu Val Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile Ser Pro 
                85                  90                  95      


Glu Leu Ala Pro Ala Leu Asp Ile Leu Gln Leu Asp Val Thr Asp Leu 
            100                 105                 110         


Ala Thr Asn Ile Trp Leu Gln Met Glu Asp Leu Arg Met Ala Pro Ala 
        115                 120                 125             


Ser Leu Pro Thr Gln Gly Thr Val Pro Thr Phe Thr Ser Ala Phe Gln 
    130                 135                 140                 


Arg Arg Ala Gly Gly Val Leu Val Val Ser Gln Leu Gln Ser Phe Leu 
145                 150                 155                 160 


Glu Leu Ala Tyr Arg Val Leu Arg Tyr Leu Ala Glu Pro 
                165                 170             


<210>  13
<211>  174
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Artificial


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (17)..(17)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (43)..(43)
<223>  Xaa = pAF

<220>
<221>  misc_feature
<222>  (121)..(121)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  misc_feature
<222>  (126)..(126)
<223>  Xaa can be any naturally occurring amino acid

<400>  13

Xaa Pro Leu Ser Pro Ala Ser Ser Leu Pro Gln Ser Phe Leu Leu Lys 
1               5                   10                  15      


Xaa Leu Glu Gln Val Arg Lys Ile Gln Ala Asp Gly Ala Glu Leu Gln 
            20                  25                  30          


Glu Arg Leu Cys Ala Thr His Lys Leu Cys Xaa Pro Gln Glu Leu Val 
        35                  40                  45              


Leu Leu Gly His Ser Leu Gly Leu Pro Gln Ala Ser Leu Ser Ser Cys 
    50                  55                  60                  


Ser Ser Gln Ala Leu Gln Leu Thr Gly Cys Leu Asn Gln Leu His Gly 
65                  70                  75                  80  


Gly Leu Val Leu Tyr Gln Gly Leu Leu Gln Ala Leu Ala Gly Ile Ser 
                85                  90                  95      


Pro Glu Leu Ala Pro Ala Leu Asp Ile Leu Gln Leu Asp Val Thr Asp 
            100                 105                 110         


Leu Ala Thr Asn Ile Trp Leu Gln Xaa Glu Asp Leu Arg Xaa Ala Pro 
        115                 120                 125             


Ala Ser Leu Pro Thr Gln Gly Thr Val Pro Thr Phe Thr Ser Ala Phe 
    130                 135                 140                 


Gln Arg Arg Ala Gly Gly Val Leu Val Val Ser Gln Leu Gln Ser Phe 
145                 150                 155                 160 


Leu Glu Leu Ala Tyr Arg Val Leu Arg Tyr Leu Ala Glu Pro 
                165                 170                 


