                         SEQUENCE LISTING

<110>  Universitt Heidelberg
 
<120>  KCNK3-based gene therapy of cardiac arrhythmia

<130>  789-25 PCT

<150>  EP 17196242.6
<151>  2017-10-12

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modifed sequence


<220>
<221>  si-p/hTASK-1-1
<222>  (1)..(21)
<223>  DNA sequence corresponding to the siRNA sequence 1 directed 
       against the porcine and the human ortholog of 	TASK-1

<400>  1
gcaaggtgtt ctgcatgttc t                                                 21


<210>  2
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified sequence


<220>
<221>  si-pTASK-1-2
<222>  (1)..(21)
<223>  DNA sequence corresponding to the siRNA sequence 2 directed 
       against the porcine ortholog of TASK-1

<400>  2
tcacgctggt catgttccag a                                                 21


<210>  3
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  si-pTASK-1-3
<222>  (1)..(21)
<223>  DNA sequence corresponding to the siRNA sequence 3 directed 
       against the porcine ortholog of TASK-1

<400>  3
acgtggcctt tagcttcgtc t                                                 21


<210>  4
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  modified sequence


<220>
<221>  si-hTASK-1-2
<222>  (1)..(21)
<223>  DNA sequence corresponding to the siRNA sequence 2 directed 
       against the human ortholog of TASK-1

<400>  4
tcacgctagt catgttccag a                                                 21


<210>  5
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  si-hTASK-1-3
<222>  (1)..(21)
<223>  DNA sequence corresponding to the siRNA sequence 3 directed 
       against the human ortholog of TASK-1

<400>  5
acgtggcctt cagcttcgtc t                                                 21


<210>  6
<211>  5444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  pSSV9-TNT-miR/si-p/hTASK-1-1-IRES2-eGFP
<222>  (1)..(5444)
<223>  Plasmid carrying the ITR-flanked construct of TASK-1 siRNA 
       sequence 1 (directed against the porcine and the human TASK-1 
       ortholog) coupled to an eGFP-Reporter via IRES2 under control of 
       the cardiomyocyte specific Troponin promoter

<400>  6
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggccactcc      540

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg      600

ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat      660

cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg      720

ctctagagca agatcgaatt cacgcgtctc agtccattag gagccagtag cctggaagat      780

gtctttaccc ccagcatcag ttcaagtgga gcagcacata actcttgccc tctgccttcc      840

aagattctgg tgctgagact tatggagtgt cttggaggtt gccttctgcc ccccaaccct      900

gctcccagct ggccctccca ggcctgggtt gctggcctct gctttatcag gattctcaag      960

agggacagct ggtttatgtt gcatgactgt tccctgcata tctgctctgg ttttaaatag     1020

cttatctgag cagctggagg accacatggg cttatatggc gtggggtaca tgttcctgta     1080

gccttgtccc tggcacctgc caaaatagca gccaacaccc cccaccccca ccgccatccc     1140

cctgccccac ccgtcccctg tcgcacattc ctccctccgc agggctggct caccaggccc     1200

cagcccacat gcctgcttaa agccctctcc atcctctgcc tcacccagtc cccgctgaga     1260

ctgagcagac gcctccagga tctgtcggca gaagctttat tgcggtagtt tatcacagtt     1320

aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt     1380

ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat     1440

agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc     1500

ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct     1560

cttaaggcta gagtacttaa tacgactcac tataggctag cctcgacggt accgcggctg     1620

gaggcttgct gaaggctgta tgctgagaac atgcagaaca ccttgcgttt tggccactga     1680

ctgacgcaag gtgctgcatg ttctcaggac acaaggcctg ttactagcac tcacatggaa     1740

caaatggcgg atccctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct     1800

cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc     1860

ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga     1920

caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc     1980

ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt     2040

attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg     2100

gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc     2160

gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg     2220

tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg     2280

acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca     2340

agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg     2400

tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc     2460

acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca     2520

aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga     2580

accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc     2640

tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca     2700

tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc     2760

actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc     2820

tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc     2880

tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg     2940

gccgcgactc tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga     3000

tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg     3060

tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa     3120

ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta     3180

aaacctctac aaatgtggta aaatcgatgc tagcctagta ataaaccgga cattcgaaag     3240

gctgcggtcg aactctagtt gctctagagc atggctacgt agataagtag catggcgggt     3300

taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc     3360

gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg     3420

cctcagtgag cgagcgagcg cgcagagagg gagtggccag ctgcattaat gaatcggcca     3480

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     3540

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     3600

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     3660

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     3720

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     3780

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     3840

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     3900

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     3960

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     4020

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     4080

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     4140

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     4200

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     4260

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     4320

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     4380

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     4440

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     4500

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     4560

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     4620

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     4680

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     4740

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     4800

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     4860

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     4920

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     4980

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     5040

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     5100

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     5160

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     5220

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     5280

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     5340

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa     5400

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac                      5444


<210>  7
<211>  5444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  pSSV9-TNT-miR/si-pTASK-1-2-IRES2-eGFP
<222>  (1)..(5444)
<223>  Plasmid carrying the ITR-flanked construct of TASK-1 siRNA 
       sequence 2 (directed against the porcine TASK-1 ortholog) coupled
       to an eGFP-Reporter via IRES2 under control of the cardiomyocyte 
       specific Troponin promoter

<400>  7
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggccactcc      540

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg      600

ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat      660

cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg      720

ctctagagca agatcgaatt cacgcgtctc agtccattag gagccagtag cctggaagat      780

gtctttaccc ccagcatcag ttcaagtgga gcagcacata actcttgccc tctgccttcc      840

aagattctgg tgctgagact tatggagtgt cttggaggtt gccttctgcc ccccaaccct      900

gctcccagct ggccctccca ggcctgggtt gctggcctct gctttatcag gattctcaag      960

agggacagct ggtttatgtt gcatgactgt tccctgcata tctgctctgg ttttaaatag     1020

cttatctgag cagctggagg accacatggg cttatatggc gtggggtaca tgttcctgta     1080

gccttgtccc tggcacctgc caaaatagca gccaacaccc cccaccccca ccgccatccc     1140

cctgccccac ccgtcccctg tcgcacattc ctccctccgc agggctggct caccaggccc     1200

cagcccacat gcctgcttaa agccctctcc atcctctgcc tcacccagtc cccgctgaga     1260

ctgagcagac gcctccagga tctgtcggca gaagctttat tgcggtagtt tatcacagtt     1320

aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt     1380

ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat     1440

agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc     1500

ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct     1560

cttaaggcta gagtacttaa tacgactcac tataggctag cctcgacggt accgcggctg     1620

gaggcttgct gaaggctgta tgctgtctgg aacatgacca gcgtgagttt tggccactga     1680

ctgactcacg ctgcatgttc cagacaggac acaaggcctg ttactagcac tcacatggaa     1740

caaatggcgg atccctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct     1800

cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc     1860

ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga     1920

caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc     1980

ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt     2040

attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg     2100

gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc     2160

gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg     2220

tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg     2280

acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca     2340

agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg     2400

tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc     2460

acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca     2520

aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga     2580

accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc     2640

tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca     2700

tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc     2760

actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc     2820

tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc     2880

tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg     2940

gccgcgactc tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga     3000

tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg     3060

tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa     3120

ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta     3180

aaacctctac aaatgtggta aaatcgatgc tagcctagta ataaaccgga cattcgaaag     3240

gctgcggtcg aactctagtt gctctagagc atggctacgt agataagtag catggcgggt     3300

taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc     3360

gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg     3420

cctcagtgag cgagcgagcg cgcagagagg gagtggccag ctgcattaat gaatcggcca     3480

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     3540

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     3600

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     3660

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     3720

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     3780

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     3840

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     3900

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     3960

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     4020

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     4080

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     4140

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     4200

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     4260

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     4320

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     4380

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     4440

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     4500

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     4560

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     4620

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     4680

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     4740

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     4800

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     4860

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     4920

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     4980

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     5040

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     5100

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     5160

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     5220

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     5280

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     5340

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa     5400

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac                      5444


<210>  8
<211>  5444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  pSSV9-TNT-miR/si-pTASK-1-3-IRES2-eGFP
<222>  (1)..(5444)
<223>  Plasmid carrying the ITR-flanked construct of TASK-1 siRNA 
       sequence 3 (directed against the porcine TASK-1 ortholog) coupled
       to an eGFP-Reporter via IRES2 under control of the cardiomyocyte 
       specific Troponin promoter

<400>  8
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggccactcc      540

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg      600

ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat      660

cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg      720

ctctagagca agatcgaatt cacgcgtctc agtccattag gagccagtag cctggaagat      780

gtctttaccc ccagcatcag ttcaagtgga gcagcacata actcttgccc tctgccttcc      840

aagattctgg tgctgagact tatggagtgt cttggaggtt gccttctgcc ccccaaccct      900

gctcccagct ggccctccca ggcctgggtt gctggcctct gctttatcag gattctcaag      960

agggacagct ggtttatgtt gcatgactgt tccctgcata tctgctctgg ttttaaatag     1020

cttatctgag cagctggagg accacatggg cttatatggc gtggggtaca tgttcctgta     1080

gccttgtccc tggcacctgc caaaatagca gccaacaccc cccaccccca ccgccatccc     1140

cctgccccac ccgtcccctg tcgcacattc ctccctccgc agggctggct caccaggccc     1200

cagcccacat gcctgcttaa agccctctcc atcctctgcc tcacccagtc cccgctgaga     1260

ctgagcagac gcctccagga tctgtcggca gaagctttat tgcggtagtt tatcacagtt     1320

aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt     1380

ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat     1440

agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc     1500

ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct     1560

cttaaggcta gagtacttaa tacgactcac tataggctag cctcgacggt accgcggctg     1620

gaggcttgct gaaggctgta tgctgagacg aagctaaagg ccacgtgttt tggccactga     1680

ctgacacgtg gcctagcttc gtctcaggac acaaggcctg ttactagcac tcacatggaa     1740

caaatggcgg atccctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct     1800

cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc     1860

ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga     1920

caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc     1980

ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt     2040

attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg     2100

gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc     2160

gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg     2220

tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg     2280

acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca     2340

agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg     2400

tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc     2460

acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca     2520

aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga     2580

accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc     2640

tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca     2700

tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc     2760

actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc     2820

tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc     2880

tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg     2940

gccgcgactc tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga     3000

tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg     3060

tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa     3120

ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta     3180

aaacctctac aaatgtggta aaatcgatgc tagcctagta ataaaccgga cattcgaaag     3240

gctgcggtcg aactctagtt gctctagagc atggctacgt agataagtag catggcgggt     3300

taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc     3360

gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg     3420

cctcagtgag cgagcgagcg cgcagagagg gagtggccag ctgcattaat gaatcggcca     3480

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     3540

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     3600

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     3660

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     3720

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     3780

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     3840

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     3900

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     3960

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     4020

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     4080

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     4140

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     4200

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     4260

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     4320

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     4380

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     4440

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     4500

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     4560

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     4620

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     4680

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     4740

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     4800

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     4860

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     4920

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     4980

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     5040

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     5100

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     5160

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     5220

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     5280

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     5340

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa     5400

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac                      5444


<210>  9
<211>  5444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  pSSV9-TNT-miR/si-hTASK-1-2-IRES2-eGFP
<222>  (1)..(5444)
<223>  Plasmid carrying the ITR-flanked construct of TASK-1 siRNA 
       sequence 2 (directed against the human TASK-1 ortholog) coupled 
       to an eGFP-Reporter via IRES2 under control of the cardiomyocyte 
       specific Troponin promoter

<400>  9
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggccactcc      540

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg      600

ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat      660

cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg      720

ctctagagca agatcgaatt cacgcgtctc agtccattag gagccagtag cctggaagat      780

gtctttaccc ccagcatcag ttcaagtgga gcagcacata actcttgccc tctgccttcc      840

aagattctgg tgctgagact tatggagtgt cttggaggtt gccttctgcc ccccaaccct      900

gctcccagct ggccctccca ggcctgggtt gctggcctct gctttatcag gattctcaag      960

agggacagct ggtttatgtt gcatgactgt tccctgcata tctgctctgg ttttaaatag     1020

cttatctgag cagctggagg accacatggg cttatatggc gtggggtaca tgttcctgta     1080

gccttgtccc tggcacctgc caaaatagca gccaacaccc cccaccccca ccgccatccc     1140

cctgccccac ccgtcccctg tcgcacattc ctccctccgc agggctggct caccaggccc     1200

cagcccacat gcctgcttaa agccctctcc atcctctgcc tcacccagtc cccgctgaga     1260

ctgagcagac gcctccagga tctgtcggca gaagctttat tgcggtagtt tatcacagtt     1320

aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt     1380

ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat     1440

agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc     1500

ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct     1560

cttaaggcta gagtacttaa tacgactcac tataggctag cctcgacggt accgcggctg     1620

gaggcttgct gaaggctgta tgctgtctgg aacatgacga gcgtgagttt tggccactga     1680

ctgactcacg ctccatgttc cagacaggac acaaggcctg ttactagcac tcacatggaa     1740

caaatggcgg atccctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct     1800

cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc     1860

ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga     1920

caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc     1980

ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt     2040

attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg     2100

gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc     2160

gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg     2220

tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg     2280

acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca     2340

agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg     2400

tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc     2460

acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca     2520

aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga     2580

accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc     2640

tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca     2700

tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc     2760

actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc     2820

tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc     2880

tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg     2940

gccgcgactc tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga     3000

tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg     3060

tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa     3120

ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta     3180

aaacctctac aaatgtggta aaatcgatgc tagcctagta ataaaccgga cattcgaaag     3240

gctgcggtcg aactctagtt gctctagagc atggctacgt agataagtag catggcgggt     3300

taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc     3360

gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg     3420

cctcagtgag cgagcgagcg cgcagagagg gagtggccag ctgcattaat gaatcggcca     3480

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     3540

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     3600

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     3660

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     3720

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     3780

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     3840

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     3900

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     3960

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     4020

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     4080

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     4140

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     4200

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     4260

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     4320

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     4380

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     4440

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     4500

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     4560

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     4620

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     4680

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     4740

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     4800

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     4860

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     4920

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     4980

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     5040

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     5100

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     5160

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     5220

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     5280

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     5340

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa     5400

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac                      5444


<210>  10
<211>  5444
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  pSSV9-TNT-miR/si-hTASK-1-3-IRES2-eGFP
<222>  (1)..(5444)
<223>  Plasmid carrying the ITR-flanked construct of TASK-1 siRNA 
       sequence 3 (directed against the human TASK-1 ortholog) coupled 
       to an eGFP-Reporter via IRES2 under control of the cardiomyocyte 
       specific Troponin promoter

<400>  10
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggccactcc      540

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg      600

ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat      660

cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg      720

ctctagagca agatcgaatt cacgcgtctc agtccattag gagccagtag cctggaagat      780

gtctttaccc ccagcatcag ttcaagtgga gcagcacata actcttgccc tctgccttcc      840

aagattctgg tgctgagact tatggagtgt cttggaggtt gccttctgcc ccccaaccct      900

gctcccagct ggccctccca ggcctgggtt gctggcctct gctttatcag gattctcaag      960

agggacagct ggtttatgtt gcatgactgt tccctgcata tctgctctgg ttttaaatag     1020

cttatctgag cagctggagg accacatggg cttatatggc gtggggtaca tgttcctgta     1080

gccttgtccc tggcacctgc caaaatagca gccaacaccc cccaccccca ccgccatccc     1140

cctgccccac ccgtcccctg tcgcacattc ctccctccgc agggctggct caccaggccc     1200

cagcccacat gcctgcttaa agccctctcc atcctctgcc tcacccagtc cccgctgaga     1260

ctgagcagac gcctccagga tctgtcggca gaagctttat tgcggtagtt tatcacagtt     1320

aaattgctaa cgcagtcagt gcttctgaca caacagtctc gaacttaagc tgcagaagtt     1380

ggtcgtgagg cactgggcag gtaagtatca aggttacaag acaggtttaa ggagaccaat     1440

agaaactggg cttgtcgaga cagagaagac tcttgcgttt ctgataggca cctattggtc     1500

ttactgacat ccactttgcc tttctctcca caggtgtcca ctcccagttc aattacagct     1560

cttaaggcta gagtacttaa tacgactcac tataggctag cctcgacggt accgcggctg     1620

gaggcttgct gaaggctgta tgctgagacg aagctgaagg ccacgtgttt tggccactga     1680

ctgacacgtg gcctagcttc gtctcaggac acaaggcctg ttactagcac tcacatggaa     1740

caaatggcgg atccctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct     1800

cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc     1860

ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga     1920

caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc     1980

ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt     2040

attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg     2100

gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc     2160

gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg     2220

tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg     2280

acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca     2340

agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg     2400

tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc     2460

acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca     2520

aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga     2580

accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc     2640

tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca     2700

tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc     2760

actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc     2820

tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc     2880

tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg     2940

gccgcgactc tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga     3000

tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg     3060

tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa     3120

ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta     3180

aaacctctac aaatgtggta aaatcgatgc tagcctagta ataaaccgga cattcgaaag     3240

gctgcggtcg aactctagtt gctctagagc atggctacgt agataagtag catggcgggt     3300

taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc     3360

gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg     3420

cctcagtgag cgagcgagcg cgcagagagg gagtggccag ctgcattaat gaatcggcca     3480

acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc     3540

gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg     3600

gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa     3660

ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga     3720

cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag     3780

ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct     3840

taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg     3900

ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc     3960

ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt     4020

aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta     4080

tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac     4140

agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc     4200

ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat     4260

tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc     4320

tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt     4380

cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta     4440

aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct     4500

atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg     4560

cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga     4620

tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt     4680

atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt     4740

taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt     4800

tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat     4860

gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc     4920

cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc     4980

cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat     5040

gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag     5100

aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt     5160

accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc     5220

ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa     5280

gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg     5340

aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa     5400

taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac                      5444


<210>  11
<211>  5859
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  pSSV9-CMV/MLC-miR/si-p/hTASK-1-1-IRES2-eGFP
<222>  (1)..(5859)
<223>  Plasmid carrying the ITR-flanked construct of TASK-1 siRNA 
       sequence 1 (directed against the porcine and the human TASK-1 
       ortholog) coupled to an eGFP-Reporter via IRES2 under control of 
       the cardiomyocyte specific CMV-enhanced MLC260 promoter

<400>  11
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggccactcc      540

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg      600

ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat      660

cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg      720

ctctagagca agatcgaatt cacgcgtcgg cggccgcttc gagctcgccc gacattgatt      780

attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga      840

gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg      900

cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg      960

acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca     1020

tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc     1080

ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc     1140

tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc     1200

acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa     1260

tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca tgcggccgct ctaggaccca     1320

gagcacagag catcgttccc aggccaggcc ccagccactg tctctttaac cttgaaggca     1380

tttttgggtc tcacgtgtcc acccaggcgg gtgtcggact ttgaacggct cttacttcag     1440

aagaacggca tggggtgggg gggcttaggt ggcctctgcc tcacctacaa ctgccaaaag     1500

tggtcatggg gttattttta accccaggga agaggtattt attgttccac agcaggggcc     1560

ggccagcagg ctccttgccc aagctttatt gcggtagttt atcacagtta aattgctaac     1620

gcagtcagtg cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc     1680

actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc     1740

ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc     1800

cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag     1860

agtacttaat acgactcact ataggctagc ctcgacggta ccgcggctgg aggcttgctg     1920

aaggctgtat gctgagaaca tgcagaacac cttgcgtttt ggccactgac tgacgcaagg     1980

tgctgcatgt tctcaggaca caaggcctgt tactagcact cacatggaac aaatggcgga     2040

tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc     2100

ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg     2160

cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca     2220

aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa     2280

gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt     2340

gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt     2400

gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca     2460

acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc     2520

ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc     2580

acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc     2640

aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta     2700

aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg     2760

accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc     2820

accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac     2880

ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac     2940

gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc     3000

atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag     3060

tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag     3120

gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac     3180

cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc     3240

acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag     3300

ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc     3360

gactctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt     3420

ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg     3480

ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca     3540

ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc     3600

tctacaaatg tggtaaaatc gatgctagcc tagtaataaa ccggacattc gaaaggctgc     3660

ggtcgaactc tagttgctct agagcatggc tacgtagata agtagcatgg cgggttaatc     3720

attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg     3780

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca     3840

gtgagcgagc gagcgcgcag agagggagtg gccagctgca ttaatgaatc ggccaacgcg     3900

cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc     3960

gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat     4020

ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca     4080

ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc     4140

atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc     4200

aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg     4260

gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta     4320

ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg     4380

ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac     4440

acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag     4500

gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat     4560

ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat     4620

ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc     4680

gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt     4740

ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct     4800

agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt     4860

ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc     4920

gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac     4980

catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat     5040

cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg     5100

cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata     5160

gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta     5220

tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt     5280

gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag     5340

tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa     5400

gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc     5460

gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt     5520

taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc     5580

tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta     5640

ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa     5700

taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca     5760

tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac     5820

aaataggggt tccgcgcaca tttccccgaa aagtgccac                            5859


<210>  12
<211>  5859
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  pSSV9-CMV/MLC-miR/si-pTASK-1-2-IRES2-eGFP
<222>  (1)..(5859)
<223>  Plasmid carrying the ITR-flanked construct of TASK-1 siRNA 
       sequence 2 (directed against the porcine TASK-1 ortholog) coupled
       to an eGFP-Reporter via IRES-2 under control of the cardiomyocyte
       specific CMV-enhanced MLC260 promoter

<400>  12
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggccactcc      540

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg      600

ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat      660

cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg      720

ctctagagca agatcgaatt cacgcgtcgg cggccgcttc gagctcgccc gacattgatt      780

attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga      840

gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg      900

cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg      960

acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca     1020

tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc     1080

ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc     1140

tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc     1200

acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa     1260

tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca tgcggccgct ctaggaccca     1320

gagcacagag catcgttccc aggccaggcc ccagccactg tctctttaac cttgaaggca     1380

tttttgggtc tcacgtgtcc acccaggcgg gtgtcggact ttgaacggct cttacttcag     1440

aagaacggca tggggtgggg gggcttaggt ggcctctgcc tcacctacaa ctgccaaaag     1500

tggtcatggg gttattttta accccaggga agaggtattt attgttccac agcaggggcc     1560

ggccagcagg ctccttgccc aagctttatt gcggtagttt atcacagtta aattgctaac     1620

gcagtcagtg cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc     1680

actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc     1740

ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc     1800

cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag     1860

agtacttaat acgactcact ataggctagc ctcgacggta ccgcggctgg aggcttgctg     1920

aaggctgtat gctgtctgga acatgaccag cgtgagtttt ggccactgac tgactcacgc     1980

tgcatgttcc agacaggaca caaggcctgt tactagcact cacatggaac aaatggcgga     2040

tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc     2100

ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg     2160

cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca     2220

aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa     2280

gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt     2340

gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt     2400

gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca     2460

acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc     2520

ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc     2580

acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc     2640

aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta     2700

aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg     2760

accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc     2820

accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac     2880

ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac     2940

gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc     3000

atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag     3060

tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag     3120

gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac     3180

cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc     3240

acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag     3300

ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc     3360

gactctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt     3420

ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg     3480

ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca     3540

ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc     3600

tctacaaatg tggtaaaatc gatgctagcc tagtaataaa ccggacattc gaaaggctgc     3660

ggtcgaactc tagttgctct agagcatggc tacgtagata agtagcatgg cgggttaatc     3720

attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg     3780

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca     3840

gtgagcgagc gagcgcgcag agagggagtg gccagctgca ttaatgaatc ggccaacgcg     3900

cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc     3960

gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat     4020

ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca     4080

ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc     4140

atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc     4200

aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg     4260

gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta     4320

ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg     4380

ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac     4440

acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag     4500

gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat     4560

ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat     4620

ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc     4680

gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt     4740

ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct     4800

agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt     4860

ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc     4920

gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac     4980

catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat     5040

cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg     5100

cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata     5160

gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta     5220

tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt     5280

gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag     5340

tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa     5400

gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc     5460

gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt     5520

taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc     5580

tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta     5640

ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa     5700

taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca     5760

tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac     5820

aaataggggt tccgcgcaca tttccccgaa aagtgccac                            5859


<210>  13
<211>  5859
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  pSSV9-CMV/MLC-miR/si-pTASK-1-3-IRES2-eGFP
<222>  (1)..(5859)
<223>  Plasmid carrying the ITR-flanked construct of TASK-1 siRNA 
       sequence 3 (directed against the porcine TASK-1 ortholog) coupled
       to an eGFP-Reporter via IRES2 under control of the cardiomyocyte 
       specific CMV enhanced MLC260 promoter

<400>  13
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggccactcc      540

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg      600

ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat      660

cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg      720

ctctagagca agatcgaatt cacgcgtcgg cggccgcttc gagctcgccc gacattgatt      780

attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga      840

gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg      900

cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg      960

acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca     1020

tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc     1080

ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc     1140

tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc     1200

acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa     1260

tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca tgcggccgct ctaggaccca     1320

gagcacagag catcgttccc aggccaggcc ccagccactg tctctttaac cttgaaggca     1380

tttttgggtc tcacgtgtcc acccaggcgg gtgtcggact ttgaacggct cttacttcag     1440

aagaacggca tggggtgggg gggcttaggt ggcctctgcc tcacctacaa ctgccaaaag     1500

tggtcatggg gttattttta accccaggga agaggtattt attgttccac agcaggggcc     1560

ggccagcagg ctccttgccc aagctttatt gcggtagttt atcacagtta aattgctaac     1620

gcagtcagtg cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc     1680

actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc     1740

ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc     1800

cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag     1860

agtacttaat acgactcact ataggctagc ctcgacggta ccgcggctgg aggcttgctg     1920

aaggctgtat gctgagacga agctaaaggc cacgtgtttt ggccactgac tgacacgtgg     1980

cctagcttcg tctcaggaca caaggcctgt tactagcact cacatggaac aaatggcgga     2040

tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc     2100

ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg     2160

cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca     2220

aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa     2280

gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt     2340

gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt     2400

gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca     2460

acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc     2520

ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc     2580

acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc     2640

aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta     2700

aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg     2760

accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc     2820

accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac     2880

ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac     2940

gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc     3000

atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag     3060

tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag     3120

gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac     3180

cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc     3240

acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag     3300

ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc     3360

gactctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt     3420

ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg     3480

ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca     3540

ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc     3600

tctacaaatg tggtaaaatc gatgctagcc tagtaataaa ccggacattc gaaaggctgc     3660

ggtcgaactc tagttgctct agagcatggc tacgtagata agtagcatgg cgggttaatc     3720

attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg     3780

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca     3840

gtgagcgagc gagcgcgcag agagggagtg gccagctgca ttaatgaatc ggccaacgcg     3900

cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc     3960

gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat     4020

ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca     4080

ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc     4140

atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc     4200

aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg     4260

gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta     4320

ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg     4380

ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac     4440

acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag     4500

gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat     4560

ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat     4620

ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc     4680

gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt     4740

ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct     4800

agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt     4860

ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc     4920

gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac     4980

catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat     5040

cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg     5100

cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata     5160

gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta     5220

tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt     5280

gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag     5340

tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa     5400

gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc     5460

gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt     5520

taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc     5580

tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta     5640

ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa     5700

taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca     5760

tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac     5820

aaataggggt tccgcgcaca tttccccgaa aagtgccac                            5859


<210>  14
<211>  5859
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  pSSV9-CMV/MLC-miR/si-hTASK-1-2-IRES2-eGFP
<222>  (1)..(5859)
<223>  Plasmid carrying the ITR-flanked construct of TASK-1 siRNA 
       sequence 2 (directed against the human TASK-1 ortholog) coupled 
       to an eGFP-Reporter via IRES2 under control of the cardiomyocyte 
       specific CMV enhanced MLC260 promoter

<400>  14
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggccactcc      540

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg      600

ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat      660

cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg      720

ctctagagca agatcgaatt cacgcgtcgg cggccgcttc gagctcgccc gacattgatt      780

attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga      840

gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg      900

cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg      960

acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca     1020

tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc     1080

ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc     1140

tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc     1200

acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa     1260

tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca tgcggccgct ctaggaccca     1320

gagcacagag catcgttccc aggccaggcc ccagccactg tctctttaac cttgaaggca     1380

tttttgggtc tcacgtgtcc acccaggcgg gtgtcggact ttgaacggct cttacttcag     1440

aagaacggca tggggtgggg gggcttaggt ggcctctgcc tcacctacaa ctgccaaaag     1500

tggtcatggg gttattttta accccaggga agaggtattt attgttccac agcaggggcc     1560

ggccagcagg ctccttgccc aagctttatt gcggtagttt atcacagtta aattgctaac     1620

gcagtcagtg cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc     1680

actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc     1740

ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc     1800

cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag     1860

agtacttaat acgactcact ataggctagc ctcgacggta ccgcggctgg aggcttgctg     1920

aaggctgtat gctgtctgga acatgacgag cgtgagtttt ggccactgac tgactcacgc     1980

tccatgttcc agacaggaca caaggcctgt tactagcact cacatggaac aaatggcgga     2040

tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc     2100

ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg     2160

cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca     2220

aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa     2280

gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt     2340

gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt     2400

gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca     2460

acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc     2520

ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc     2580

acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc     2640

aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta     2700

aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg     2760

accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc     2820

accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac     2880

ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac     2940

gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc     3000

atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag     3060

tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag     3120

gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac     3180

cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc     3240

acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag     3300

ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc     3360

gactctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt     3420

ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg     3480

ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca     3540

ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc     3600

tctacaaatg tggtaaaatc gatgctagcc tagtaataaa ccggacattc gaaaggctgc     3660

ggtcgaactc tagttgctct agagcatggc tacgtagata agtagcatgg cgggttaatc     3720

attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg     3780

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca     3840

gtgagcgagc gagcgcgcag agagggagtg gccagctgca ttaatgaatc ggccaacgcg     3900

cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc     3960

gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat     4020

ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca     4080

ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc     4140

atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc     4200

aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg     4260

gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta     4320

ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg     4380

ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac     4440

acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag     4500

gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat     4560

ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat     4620

ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc     4680

gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt     4740

ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct     4800

agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt     4860

ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc     4920

gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac     4980

catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat     5040

cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg     5100

cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata     5160

gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta     5220

tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt     5280

gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag     5340

tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa     5400

gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc     5460

gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt     5520

taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc     5580

tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta     5640

ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa     5700

taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca     5760

tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac     5820

aaataggggt tccgcgcaca tttccccgaa aagtgccac                            5859


<210>  15
<211>  5859
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  pSSV9-CMV/MLC-miR/si-hTASK-1-3-IRES2-eGFP
<222>  (1)..(5859)
<223>  Plasmid carrying the ITR-flanked construct of TASK-1 siRNA 
       sequence (directed against the human TASK-1 ortholog) coupled to 
       an eGFP-Reporter via IRES2 under control of the cardiomyocyte 
       specific CMV-enhanced MLC260 promoter

<400>  15
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc       60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga      120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc      180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc      240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag      300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa      360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac      420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg      480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggccactcc      540

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg      600

ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat      660

cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg      720

ctctagagca agatcgaatt cacgcgtcgg cggccgcttc gagctcgccc gacattgatt      780

attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga      840

gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg      900

cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg      960

acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca     1020

tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc     1080

ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc     1140

tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc     1200

acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa     1260

tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca tgcggccgct ctaggaccca     1320

gagcacagag catcgttccc aggccaggcc ccagccactg tctctttaac cttgaaggca     1380

tttttgggtc tcacgtgtcc acccaggcgg gtgtcggact ttgaacggct cttacttcag     1440

aagaacggca tggggtgggg gggcttaggt ggcctctgcc tcacctacaa ctgccaaaag     1500

tggtcatggg gttattttta accccaggga agaggtattt attgttccac agcaggggcc     1560

ggccagcagg ctccttgccc aagctttatt gcggtagttt atcacagtta aattgctaac     1620

gcagtcagtg cttctgacac aacagtctcg aacttaagct gcagaagttg gtcgtgaggc     1680

actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc     1740

ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc     1800

cactttgcct ttctctccac aggtgtccac tcccagttca attacagctc ttaaggctag     1860

agtacttaat acgactcact ataggctagc ctcgacggta ccgcggctgg aggcttgctg     1920

aaggctgtat gctgagacga agctgaaggc cacgtgtttt ggccactgac tgacacgtgg     1980

cctagcttcg tctcaggaca caaggcctgt tactagcact cacatggaac aaatggcgga     2040

tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc     2100

ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg     2160

cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca     2220

aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa     2280

gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt     2340

gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt     2400

gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca     2460

acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc     2520

ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc     2580

acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc     2640

aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta     2700

aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg     2760

accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc     2820

accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac     2880

ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac     2940

gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc     3000

atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag     3060

tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag     3120

gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac     3180

cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc     3240

acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag     3300

ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc     3360

gactctagag tcggggcggc cggccgcttc gagcagacat gataagatac attgatgagt     3420

ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg     3480

ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca     3540

ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc     3600

tctacaaatg tggtaaaatc gatgctagcc tagtaataaa ccggacattc gaaaggctgc     3660

ggtcgaactc tagttgctct agagcatggc tacgtagata agtagcatgg cgggttaatc     3720

attaactaca aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg     3780

ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca     3840

gtgagcgagc gagcgcgcag agagggagtg gccagctgca ttaatgaatc ggccaacgcg     3900

cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc     3960

gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat     4020

ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca     4080

ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc     4140

atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc     4200

aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg     4260

gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta     4320

ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg     4380

ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac     4440

acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag     4500

gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat     4560

ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat     4620

ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc     4680

gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt     4740

ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct     4800

agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt     4860

ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc     4920

gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac     4980

catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat     5040

cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg     5100

cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata     5160

gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta     5220

tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt     5280

gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag     5340

tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa     5400

gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc     5460

gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt     5520

taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc     5580

tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta     5640

ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa     5700

taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca     5760

tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac     5820

aaataggggt tccgcgcaca tttccccgaa aagtgccac                            5859


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  hTASK-1
<222>  (1)..(21)
<223>  hTASK-1 siRNA from Hao and Li

<400>  16
ccttcagctt cgtgtacatt t                                                 21


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  hTASK-1
<222>  (1)..(21)
<223>  hTASK-1 siRNA from Olschewski et al.

<400>  17
tcaccgtcat caccaccatt t                                                 21


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  hTASK-1
<222>  (1)..(21)
<223>  hTASK-1 siRNA from Gurney and Hunter (

<400>  18
aacaccttcg tgaggtacct t                                                 21


<210>  19
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified sequence


<220>
<221>  hTASK-1
<222>  (1)..(21)
<223>  hTASK-1 siRNA from Tang et al.

<400>  19
tcaccgtcat caccaccatt t                                                 21


<210>  20
<211>  60
<212>  DNA
<213>  Homo sapiens


<220>
<221>  humanTASK-1
<222>  (1)..(60)
<223>  human TASK-1 sequence; coding sequence used for design of siRNA 1

<400>  20
cccagcacgg atggcggcaa ggtgttctgc atgttctacg cgctgctggg catcccgctc       60


<210>  21
<211>  60
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  ratTASK-1
<222>  (1)..(60)
<223>  rat TASK-1 sequence, coding sequence used for design of siRNA 1

<400>  21
cccagcacgg acggaggcaa ggtgttctgc atgttctacg cgctgctggg catcccgctc       60


<210>  22
<211>  60
<212>  DNA
<213>  Sus scrofa


<220>
<221>  porcineTASK-1
<222>  (1)..(60)
<223>  porcine TASK-1 sequence, coding sequence used for design of siRNA
       1

<400>  22
cccagcacag acggcggcaa ggtgttctgc atgttctacg cgctcttggg catcccgctc       60


<210>  23
<211>  60
<212>  DNA
<213>  homo sapiens


<220>
<221>  humanTASK-1
<222>  (1)..(60)
<223>  human TASK-1 sequence; coding sequence used for design of siRNA 2

<400>  23
ccgctcacgc tcgtcatgtt ccagagcctg ggcgagcgca tcaacacctt ggtgaggtac       60


<210>  24
<211>  60
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  ratTASK-1
<222>  (1)..(60)
<223>  rat TASK-1 sequence, coding sequence used for design of siRNA 2

<400>  24
ccgctcacac tagtcatgtt ccagagcctg ggtgaacgca tcaacacctt cgtgaggtac       60


<210>  25
<211>  60
<212>  DNA
<213>  Sus scrofa


<220>
<221>  porcineTASK-1
<222>  (1)..(60)
<223>  porcine TASK-1 sequence, coding sequence used for design of siRNA
       2

<400>  25
ccgctcacgc tggtcatgtt ccagagcctg ggcgagcgca tcaacacctt cgtgaagtac       60


<210>  26
<211>  60
<212>  DNA
<213>  Homo sapiens


<220>
<221>  humanTASK-1
<222>  (1)..(60)
<223>  human TASK-1 sequence; coding sequence used for design of siRNA 3

<400>  26
gcagacgtgg ccttcagctt cgtctacatc cttacgggcc tcacggtcat cggcgccttc       60


<210>  27
<211>  60
<212>  DNA
<213>  Rattus norvegicus


<220>
<221>  ratTASK-1
<222>  (1)..(60)
<223>  rat TASK-1 sequence, coding sequence used for design of siRNA 3

<400>  27
gcagatgtgg ccttcagctt cgtgtacatc ctcacgggcc tcacggtcat cggcgccttc       60


<210>  28
<211>  60
<212>  DNA
<213>  Sus scrofa


<220>
<221>  TASK-1porcine
<222>  (1)..(60)
<223>  porcine TASK-1 sequence, coding sequence used for design of siRNA
       3

<400>  28
gcagacgtgg cctttagctt cgtctacatc ctcacgggcc tcacggtcat cggcgccttt       60


