                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  POLYPEPTIDES HAVING PROTEASE ACTIVITY AND POLYNUCLEOTIDES 
       ENCODING SAME

<130>  14484-WO-PCT

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  1278
<212>  DNA
<213>  Palaeococcus ferrophilus

<400>  1
atgagaaagt acattgcagt ggcgctggcc atcgcgatgt tcgcagggct ttttgtggtt       60

cccaccatgg ccgagaggcc cgagatggtc agggtcgtgg ttcacataga cagatccagc      120

tttaatcccg cgggggtttt gaacctcggg gggcacgtcg tgtaccagtt ccgcctgatt      180

gacgccgccg tggttgaggt tccctccacc gccgtaggga ggctcaagag gctgcccggc      240

gttgagaaag tcgagttcga ccatcaggca gttatcctca agggcaagcc ttcgggggct      300

gggaagccca agccaagcca gccaccccaa gaggttccat ggggaataga gcgcgttaag      360

gctccaagtg tgtggagcac aaccgatgga tcaagcaacg gggtcatcca ggttgcaatc      420

ctcgacactg gaatagacta cgaccacccg gacctcgccg ccaacctcgc ctggggtgtg      480

agcacgctta ggggtagggt ttctaccaaa ccgaaggact acagagacca gaacggtcac      540

ggaacccacg tggcgggaac aatagcggcc ctcaacaacg acatcggagt tgtcggcgtc      600

gctcctggag tccagattta cgcgattagg gttctcgacg cgagcggaag gggttcgtac      660

agtgacatag ccataggaat tgagcaggcc atcctcggcc cggacggagt tgcagacaag      720

gatggagatg gaataatagc gggcgatccc gacgatgacg cggcagaggt aataagcatg      780

tccctcggag gctccgccga cgacagctac ctccacgata tgataatcca agcctacaac      840

gctggaatag tcatcgtagc cgccagcggc aacgagggtg cctcaagccc gagctaccct      900

gccgcttacc cggaggttat tgccgtcggc gcgagtgaca tcaacgacaa catcgcgagc      960

ttcagcaacc gccagcctga ggtcagcgcc ccgggcgttg acgttctcag cacctacccg     1020

gacgacactt acaagactct cagcggaacg agcatggcca cacctcacgt gagcggcgtc     1080

gtggcactaa tacaagcggc tcacttcaac aagtacggca cgatccttcc ggttggaacc     1140

tttgacgaca tgagcaagaa caccgtgagg ggaatcctcc acatcacggc tgacgacctt     1200

ggaagcccag gatgggatgt agactacggc tacggtattg tgagggcaga cctcgccgtc     1260

caggccgcgc tcggatga                                                   1278


<210>  2
<211>  425
<212>  PRT
<213>  Palaeococcus ferrophilus

<400>  2

Met Arg Lys Tyr Ile Ala Val Ala Leu Ala Ile Ala Met Phe Ala Gly 
1               5                   10                  15      


Leu Phe Val Val Pro Thr Met Ala Glu Arg Pro Glu Met Val Arg Val 
            20                  25                  30          


Val Val His Ile Asp Arg Ser Ser Phe Asn Pro Ala Gly Val Leu Asn 
        35                  40                  45              


Leu Gly Gly His Val Val Tyr Gln Phe Arg Leu Ile Asp Ala Ala Val 
    50                  55                  60                  


Val Glu Val Pro Ser Thr Ala Val Gly Arg Leu Lys Arg Leu Pro Gly 
65                  70                  75                  80  


Val Glu Lys Val Glu Phe Asp His Gln Ala Val Ile Leu Lys Gly Lys 
                85                  90                  95      


Pro Ser Gly Ala Gly Lys Pro Lys Pro Ser Gln Pro Pro Gln Glu Val 
            100                 105                 110         


Pro Trp Gly Ile Glu Arg Val Lys Ala Pro Ser Val Trp Ser Thr Thr 
        115                 120                 125             


Asp Gly Ser Ser Asn Gly Val Ile Gln Val Ala Ile Leu Asp Thr Gly 
    130                 135                 140                 


Ile Asp Tyr Asp His Pro Asp Leu Ala Ala Asn Leu Ala Trp Gly Val 
145                 150                 155                 160 


Ser Thr Leu Arg Gly Arg Val Ser Thr Lys Pro Lys Asp Tyr Arg Asp 
                165                 170                 175     


Gln Asn Gly His Gly Thr His Val Ala Gly Thr Ile Ala Ala Leu Asn 
            180                 185                 190         


Asn Asp Ile Gly Val Val Gly Val Ala Pro Gly Val Gln Ile Tyr Ala 
        195                 200                 205             


Ile Arg Val Leu Asp Ala Ser Gly Arg Gly Ser Tyr Ser Asp Ile Ala 
    210                 215                 220                 


Ile Gly Ile Glu Gln Ala Ile Leu Gly Pro Asp Gly Val Ala Asp Lys 
225                 230                 235                 240 


Asp Gly Asp Gly Ile Ile Ala Gly Asp Pro Asp Asp Asp Ala Ala Glu 
                245                 250                 255     


Val Ile Ser Met Ser Leu Gly Gly Ser Ala Asp Asp Ser Tyr Leu His 
            260                 265                 270         


Asp Met Ile Ile Gln Ala Tyr Asn Ala Gly Ile Val Ile Val Ala Ala 
        275                 280                 285             


Ser Gly Asn Glu Gly Ala Ser Ser Pro Ser Tyr Pro Ala Ala Tyr Pro 
    290                 295                 300                 


Glu Val Ile Ala Val Gly Ala Ser Asp Ile Asn Asp Asn Ile Ala Ser 
305                 310                 315                 320 


Phe Ser Asn Arg Gln Pro Glu Val Ser Ala Pro Gly Val Asp Val Leu 
                325                 330                 335     


Ser Thr Tyr Pro Asp Asp Thr Tyr Lys Thr Leu Ser Gly Thr Ser Met 
            340                 345                 350         


Ala Thr Pro His Val Ser Gly Val Val Ala Leu Ile Gln Ala Ala His 
        355                 360                 365             


Phe Asn Lys Tyr Gly Thr Ile Leu Pro Val Gly Thr Phe Asp Asp Met 
    370                 375                 380                 


Ser Lys Asn Thr Val Arg Gly Ile Leu His Ile Thr Ala Asp Asp Leu 
385                 390                 395                 400 


Gly Ser Pro Gly Trp Asp Val Asp Tyr Gly Tyr Gly Ile Val Arg Ala 
                405                 410                 415     


Asp Leu Ala Val Gln Ala Ala Leu Gly 
            420                 425 


<210>  3
<211>  1209
<212>  DNA
<213>  Artificial

<220>
<223>  Codon optimized sequence

<400>  3
atggaaagac cggaaatggt tagagttgtt gtccatattg atcgctcatc atttaatccg       60

gcaggcgttc tgaatctggg aggccatgtt gtttatcagt ttagactgat tgatgcagca      120

gttgttgaag ttccgtcaac agcagttggc agactgaaaa gactgcctgg cgttgaaaaa      180

gtcgaatttg atcatcaagc ggtcatcctg aaaggcaaac cgtcaggcgc aggcaaaccg      240

aaaccgtcac aaccgcctca agaagttcct tggggcattg aaagagttaa agcaccgtca      300

gtttggtcaa caacggatgg ctcatcaaat ggcgttattc aagttgcaat tctggataca      360

ggcatcgact atgatcatcc ggatctggca gcaaatctgg catggggagt ttcaacactg      420

agaggcagag tttcaacaaa accgaaagat tatagagatc agaatggcca tggcacacat      480

gttgcaggca caattgcagc actgaataat gatattggcg ttgttggagt tgcacctggc      540

gttcaaattt atgcaattag agttctggat gcatcaggca gaggctcata ttcagatatt      600

gcaattggca ttgaacaggc aattctggga ccggatggcg ttgcagataa agatggcgac      660

ggcattattg caggcgatcc tgatgatgat gcagcggaag ttatttcaat gtcactggga      720

ggctcagcag atgattcata tcttcatgat atgatcatcc aggcgtataa tgcaggcatt      780

gttattgttg cagcatcagg caatgaaggc gcatcatcac cgtcatatcc ggcagcatat      840

ccggaagtta ttgcagttgg cgcatcagat attaacgata acattgcgag ctttagcaat      900

agacaaccgg aagtttcagc acctggcgtc gatgttctgt caacatatcc ggatgatacg      960

tataaaacac tgtcaggcac atcaatggca acaccgcatg tttcaggcgt tgttgcactg     1020

attcaagcag cacattttaa caaatatggc acaattctgc cggtcggcac atttgatgat     1080

atgagcaaaa atacagtccg tggcattctg catattacag cagatgatct gggctcaccg     1140

ggatgggatg ttgattatgg ctatggcatt gttagagcag atctggcagt tcaagcagcg     1200

ctgggctaa                                                             1209


<210>  4
<211>  515
<212>  PRT
<213>  Bacillus stearothermophilus

<400>  4

Ala Ala Pro Phe Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr Leu 
1               5                   10                  15      


Pro Asp Asp Gly Thr Leu Trp Thr Lys Val Ala Asn Glu Ala Asn Asn 
            20                  25                  30          


Leu Ser Ser Leu Gly Ile Thr Ala Leu Trp Leu Pro Pro Ala Tyr Lys 
        35                  40                  45              


Gly Thr Ser Arg Ser Asp Val Gly Tyr Gly Val Tyr Asp Leu Tyr Asp 
    50                  55                  60                  


Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr 
65                  70                  75                  80  


Lys Ala Gln Tyr Leu Gln Ala Ile Gln Ala Ala His Ala Ala Gly Met 
                85                  90                  95      


Gln Val Tyr Ala Asp Val Val Phe Asp His Lys Gly Gly Ala Asp Gly 
            100                 105                 110         


Thr Glu Trp Val Asp Ala Val Glu Val Asn Pro Ser Asp Arg Asn Gln 
        115                 120                 125             


Glu Ile Ser Gly Thr Tyr Gln Ile Gln Ala Trp Thr Lys Phe Asp Phe 
    130                 135                 140                 


Pro Gly Arg Gly Asn Thr Tyr Ser Ser Phe Lys Trp Arg Trp Tyr His 
145                 150                 155                 160 


Phe Asp Gly Val Asp Trp Asp Glu Ser Arg Lys Leu Ser Arg Ile Tyr 
                165                 170                 175     


Lys Phe Arg Gly Ile Gly Lys Ala Trp Asp Trp Glu Val Asp Thr Glu 
            180                 185                 190         


Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Leu Asp Met Asp His 
        195                 200                 205             


Pro Glu Val Val Thr Glu Leu Lys Asn Trp Gly Lys Trp Tyr Val Asn 
    210                 215                 220                 


Thr Thr Asn Ile Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys 
225                 230                 235                 240 


Phe Ser Phe Phe Pro Asp Trp Leu Ser Tyr Val Arg Ser Gln Thr Gly 
                245                 250                 255     


Lys Pro Leu Phe Thr Val Gly Glu Tyr Trp Ser Tyr Asp Ile Asn Lys 
            260                 265                 270         


Leu His Asn Tyr Ile Thr Lys Thr Asn Gly Thr Met Ser Leu Phe Asp 
        275                 280                 285             


Ala Pro Leu His Asn Lys Phe Tyr Thr Ala Ser Lys Ser Gly Gly Ala 
    290                 295                 300                 


Phe Asp Met Arg Thr Leu Met Thr Asn Thr Leu Met Lys Asp Gln Pro 
305                 310                 315                 320 


Thr Leu Ala Val Thr Phe Val Asp Asn His Asp Thr Glu Pro Gly Gln 
                325                 330                 335     


Ala Leu Gln Ser Trp Val Asp Pro Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         


Phe Ile Leu Thr Arg Gln Glu Gly Tyr Pro Cys Val Phe Tyr Gly Asp 
        355                 360                 365             


Tyr Tyr Gly Ile Pro Gln Tyr Asn Ile Pro Ser Leu Lys Ser Lys Ile 
    370                 375                 380                 


Asp Pro Leu Leu Ile Ala Arg Arg Asp Tyr Ala Tyr Gly Thr Gln His 
385                 390                 395                 400 


Asp Tyr Leu Asp His Ser Asp Ile Ile Gly Trp Thr Arg Glu Gly Val 
                405                 410                 415     


Thr Glu Lys Pro Gly Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro 
            420                 425                 430         


Gly Gly Ser Lys Trp Met Tyr Val Gly Lys Gln His Ala Gly Lys Val 
        435                 440                 445             


Phe Tyr Asp Leu Thr Gly Asn Arg Ser Asp Thr Val Thr Ile Asn Ser 
    450                 455                 460                 


Asp Gly Trp Gly Glu Phe Lys Val Asn Gly Gly Ser Val Ser Val Trp 
465                 470                 475                 480 


Val Pro Arg Lys Thr Thr Val Ser Thr Ile Ala Arg Pro Ile Thr Thr 
                485                 490                 495     


Arg Pro Trp Thr Gly Glu Phe Val Arg Trp Thr Glu Pro Arg Leu Val 
            500                 505                 510         


Ala Trp Pro 
        515 


<210>  5
<211>  591
<212>  PRT
<213>  Talaromyces emersonii

<400>  5

Ala Thr Gly Ser Leu Asp Ser Phe Leu Ala Thr Glu Thr Pro Ile Ala 
1               5                   10                  15      


Leu Gln Gly Val Leu Asn Asn Ile Gly Pro Asn Gly Ala Asp Val Ala 
            20                  25                  30          


Gly Ala Ser Ala Gly Ile Val Val Ala Ser Pro Ser Arg Ser Asp Pro 
        35                  40                  45              


Asn Tyr Phe Tyr Ser Trp Thr Arg Asp Ala Ala Leu Thr Ala Lys Tyr 
    50                  55                  60                  


Leu Val Asp Ala Phe Ile Ala Gly Asn Lys Asp Leu Glu Gln Thr Ile 
65                  70                  75                  80  


Gln Gln Tyr Ile Ser Ala Gln Ala Lys Val Gln Thr Ile Ser Asn Pro 
                85                  90                  95      


Ser Gly Asp Leu Ser Thr Gly Gly Leu Gly Glu Pro Lys Phe Asn Val 
            100                 105                 110         


Asn Glu Thr Ala Phe Thr Gly Pro Trp Gly Arg Pro Gln Arg Asp Gly 
        115                 120                 125             


Pro Ala Leu Arg Ala Thr Ala Leu Ile Ala Tyr Ala Asn Tyr Leu Ile 
    130                 135                 140                 


Asp Asn Gly Glu Ala Ser Thr Ala Asp Glu Ile Ile Trp Pro Ile Val 
145                 150                 155                 160 


Gln Asn Asp Leu Ser Tyr Ile Thr Gln Tyr Trp Asn Ser Ser Thr Phe 
                165                 170                 175     


Asp Leu Trp Glu Glu Val Glu Gly Ser Ser Phe Phe Thr Thr Ala Val 
            180                 185                 190         


Gln His Arg Ala Leu Val Glu Gly Asn Ala Leu Ala Thr Arg Leu Asn 
        195                 200                 205             


His Thr Cys Ser Asn Cys Val Ser Gln Ala Pro Gln Val Leu Cys Phe 
    210                 215                 220                 


Leu Gln Ser Tyr Trp Thr Gly Ser Tyr Val Leu Ala Asn Phe Gly Gly 
225                 230                 235                 240 


Ser Gly Arg Ser Gly Lys Asp Val Asn Ser Ile Leu Gly Ser Ile His 
                245                 250                 255     


Thr Phe Asp Pro Ala Gly Gly Cys Asp Asp Ser Thr Phe Gln Pro Cys 
            260                 265                 270         


Ser Ala Arg Ala Leu Ala Asn His Lys Val Val Thr Asp Ser Phe Arg 
        275                 280                 285             


Ser Ile Tyr Ala Ile Asn Ser Gly Ile Ala Glu Gly Ser Ala Val Ala 
    290                 295                 300                 


Val Gly Arg Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Pro Trp Tyr 
305                 310                 315                 320 


Leu Ala Thr Ala Ala Ala Ala Glu Gln Leu Tyr Asp Ala Ile Tyr Gln 
                325                 330                 335     


Trp Lys Lys Ile Gly Ser Ile Ser Ile Thr Asp Val Ser Leu Pro Phe 
            340                 345                 350         


Phe Gln Asp Ile Tyr Pro Ser Ala Ala Val Gly Thr Tyr Asn Ser Gly 
        355                 360                 365             


Ser Thr Thr Phe Asn Asp Ile Ile Ser Ala Val Gln Thr Tyr Gly Asp 
    370                 375                 380                 


Gly Tyr Leu Ser Ile Val Glu Lys Tyr Thr Pro Ser Asp Gly Ser Leu 
385                 390                 395                 400 


Thr Glu Gln Phe Ser Arg Thr Asp Gly Thr Pro Leu Ser Ala Ser Ala 
                405                 410                 415     


Leu Thr Trp Ser Tyr Ala Ser Leu Leu Thr Ala Ser Ala Arg Arg Gln 
            420                 425                 430         


Ser Val Val Pro Ala Ser Trp Gly Glu Ser Ser Ala Ser Ser Val Pro 
        435                 440                 445             


Ala Val Cys Ser Ala Thr Ser Ala Thr Gly Pro Tyr Ser Thr Ala Thr 
    450                 455                 460                 


Asn Thr Val Trp Pro Ser Ser Gly Ser Gly Ser Ser Thr Thr Thr Ser 
465                 470                 475                 480 


Ser Ala Pro Cys Thr Thr Pro Thr Ser Val Ala Val Thr Phe Asp Glu 
                485                 490                 495     


Ile Val Ser Thr Ser Tyr Gly Glu Thr Ile Tyr Leu Ala Gly Ser Ile 
            500                 505                 510         


Pro Glu Leu Gly Asn Trp Ser Thr Ala Ser Ala Ile Pro Leu Arg Ala 
        515                 520                 525             


Asp Ala Tyr Thr Asn Ser Asn Pro Leu Trp Tyr Val Thr Val Asn Leu 
    530                 535                 540                 


Pro Pro Gly Thr Ser Phe Glu Tyr Lys Phe Phe Lys Asn Gln Thr Asp 
545                 550                 555                 560 


Gly Thr Ile Val Trp Glu Asp Asp Pro Asn Arg Ser Tyr Thr Val Pro 
                565                 570                 575     


Ala Tyr Cys Gly Gln Thr Thr Ala Ile Leu Asp Asp Ser Trp Gln 
            580                 585                 590     


<210>  6
<211>  556
<212>  PRT
<213>  Gloeophyllum sepiarium

<400>  6

Gln Ser Val Asp Ser Tyr Val Ser Ser Glu Gly Pro Ile Ala Lys Ala 
1               5                   10                  15      


Gly Val Leu Ala Asn Ile Gly Pro Asn Gly Ser Lys Ala Ser Gly Ala 
            20                  25                  30          


Ser Ala Gly Val Val Val Ala Ser Pro Ser Thr Ser Asp Pro Asp Tyr 
        35                  40                  45              


Trp Tyr Thr Trp Thr Arg Asp Ser Ser Leu Val Phe Lys Ser Leu Ile 
    50                  55                  60                  


Asp Gln Tyr Thr Thr Gly Ile Asp Ser Thr Ser Ser Leu Arg Thr Leu 
65                  70                  75                  80  


Ile Asp Asp Phe Val Thr Ala Glu Ala Asn Leu Gln Gln Val Ser Asn 
                85                  90                  95      


Pro Ser Gly Thr Leu Thr Thr Gly Gly Leu Gly Glu Pro Lys Phe Asn 
            100                 105                 110         


Val Asp Glu Thr Ala Phe Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp 
        115                 120                 125             


Gly Pro Ala Leu Arg Ser Thr Ala Leu Ile Thr Tyr Gly Asn Trp Leu 
    130                 135                 140                 


Leu Ser Asn Gly Asn Thr Ser Tyr Val Thr Ser Asn Leu Trp Pro Ile 
145                 150                 155                 160 


Ile Gln Asn Asp Leu Gly Tyr Val Val Ser Tyr Trp Asn Gln Ser Thr 
                165                 170                 175     


Tyr Asp Leu Trp Glu Glu Val Asp Ser Ser Ser Phe Phe Thr Thr Ala 
            180                 185                 190         


Val Gln His Arg Ala Leu Arg Glu Gly Ala Ala Phe Ala Thr Ala Ile 
        195                 200                 205             


Gly Gln Thr Ser Gln Val Ser Ser Tyr Thr Thr Gln Ala Asp Asn Leu 
    210                 215                 220                 


Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Ser Gly Gly Tyr Ile Thr 
225                 230                 235                 240 


Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn Thr Leu Leu 
                245                 250                 255     


Ala Ser Ile His Thr Tyr Asp Pro Ser Ala Gly Cys Asp Ala Ala Thr 
            260                 265                 270         


Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr Val 
        275                 280                 285             


Asp Ser Phe Arg Ser Val Tyr Ser Ile Asn Ser Gly Val Ala Ser Asn 
    290                 295                 300                 


Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Ser Tyr Gln Gly Gly 
305                 310                 315                 320 


Asn Pro Trp Tyr Leu Thr Thr Phe Ala Val Ala Glu Gln Leu Tyr Asp 
                325                 330                 335     


Ala Leu Asn Val Trp Glu Ser Gln Gly Ser Leu Glu Val Thr Ser Thr 
            340                 345                 350         


Ser Leu Ala Phe Phe Gln Gln Phe Ser Ser Gly Val Thr Ala Gly Thr 
        355                 360                 365             


Tyr Ser Ser Ser Ser Ser Thr Tyr Ser Thr Leu Thr Ser Ala Ile Lys 
    370                 375                 380                 


Asn Phe Ala Asp Gly Phe Val Ala Ile Asn Ala Lys Tyr Thr Pro Ser 
385                 390                 395                 400 


Asn Gly Gly Leu Ala Glu Gln Tyr Ser Lys Ser Asp Gly Ser Pro Leu 
                405                 410                 415     


Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ser Ala Leu Thr Ala Phe 
            420                 425                 430         


Glu Ala Arg Asn Asn Thr Gln Phe Ala Gly Trp Gly Ala Ala Gly Leu 
        435                 440                 445             


Thr Val Pro Ser Ser Cys Ser Gly Asn Ser Gly Gly Pro Thr Val Ala 
    450                 455                 460                 


Val Thr Phe Asn Val Asn Ala Glu Thr Val Trp Gly Glu Asn Ile Tyr 
465                 470                 475                 480 


Leu Thr Gly Ser Val Asp Ala Leu Glu Asn Trp Ser Ala Asp Asn Ala 
                485                 490                 495     


Leu Leu Leu Ser Ser Ala Asn Tyr Pro Thr Trp Ser Ile Thr Val Asn 
            500                 505                 510         


Leu Pro Ala Ser Thr Ala Ile Glu Tyr Lys Tyr Ile Arg Lys Asn Asn 
        515                 520                 525             


Gly Ala Val Thr Trp Glu Ser Asp Pro Asn Asn Ser Ile Thr Thr Pro 
    530                 535                 540                 


Ala Ser Gly Ser Thr Thr Glu Asn Asp Thr Trp Arg 
545                 550                 555     


<210>  7
<211>  559
<212>  PRT
<213>  Gloeophyllum trabeum

<400>  7

Gln Ser Val Asp Ser Tyr Val Gly Ser Glu Gly Pro Ile Ala Lys Ala 
1               5                   10                  15      


Gly Val Leu Ala Asn Ile Gly Pro Asn Gly Ser Lys Ala Ser Gly Ala 
            20                  25                  30          


Ala Ala Gly Val Val Val Ala Ser Pro Ser Lys Ser Asp Pro Asp Tyr 
        35                  40                  45              


Trp Tyr Thr Trp Thr Arg Asp Ser Ser Leu Val Phe Lys Ser Leu Ile 
    50                  55                  60                  


Asp Gln Tyr Thr Thr Gly Ile Asp Ser Thr Ser Ser Leu Arg Ser Leu 
65                  70                  75                  80  


Ile Asp Ser Phe Val Ile Ala Glu Ala Asn Ile Gln Gln Val Ser Asn 
                85                  90                  95      


Pro Ser Gly Thr Leu Thr Thr Gly Gly Leu Gly Glu Pro Lys Phe Asn 
            100                 105                 110         


Val Asp Glu Thr Ala Phe Thr Gly Ala Trp Gly Arg Pro Gln Arg Asp 
        115                 120                 125             


Gly Pro Ala Leu Arg Ala Thr Ala Leu Ile Thr Tyr Gly Asn Trp Leu 
    130                 135                 140                 


Leu Ser Asn Gly Asn Thr Thr Trp Val Thr Ser Thr Leu Trp Pro Ile 
145                 150                 155                 160 


Ile Gln Asn Asp Leu Asn Tyr Val Val Gln Tyr Trp Asn Gln Thr Thr 
                165                 170                 175     


Phe Asp Leu Trp Glu Glu Val Asn Ser Ser Ser Phe Phe Thr Thr Ala 
            180                 185                 190         


Val Gln His Arg Ala Leu Arg Glu Gly Ala Ala Phe Ala Thr Lys Ile 
        195                 200                 205             


Gly Gln Thr Ser Ser Val Ser Ser Tyr Thr Thr Gln Ala Ala Asn Leu 
    210                 215                 220                 


Leu Cys Phe Leu Gln Ser Tyr Trp Asn Pro Thr Ser Gly Tyr Ile Thr 
225                 230                 235                 240 


Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn Thr Leu Leu 
                245                 250                 255     


Ala Ser Ile His Thr Tyr Asp Pro Ser Ala Gly Cys Asp Ala Thr Thr 
            260                 265                 270         


Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr Val 
        275                 280                 285             


Asp Ser Phe Arg Ser Val Tyr Ser Ile Asn Ser Gly Ile Ala Ser Asn 
    290                 295                 300                 


Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Ser Tyr Gln Gly Gly 
305                 310                 315                 320 


Asn Pro Trp Tyr Leu Thr Thr Phe Ala Val Ala Glu Gln Leu Tyr Asp 
                325                 330                 335     


Ala Leu Asn Val Trp Ala Ala Gln Gly Ser Leu Asn Val Thr Ser Ile 
            340                 345                 350         


Ser Leu Pro Phe Phe Gln Gln Phe Ser Ser Ser Val Thr Ala Gly Thr 
        355                 360                 365             


Tyr Ala Ser Ser Ser Thr Thr Tyr Thr Thr Leu Thr Ser Ala Ile Lys 
    370                 375                 380                 


Ser Phe Ala Asp Gly Phe Val Ala Ile Asn Ala Gln Tyr Thr Pro Ser 
385                 390                 395                 400 


Asn Gly Gly Leu Ala Glu Gln Phe Ser Arg Ser Asn Gly Ala Pro Val 
                405                 410                 415     


Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ser Ala Leu Thr Ala Phe 
            420                 425                 430         


Glu Ala Arg Asn Asn Thr Gln Phe Ala Gly Trp Gly Ala Val Gly Leu 
        435                 440                 445             


Thr Val Pro Thr Ser Cys Ser Ser Asn Ser Gly Gly Gly Gly Gly Ser 
    450                 455                 460                 


Thr Val Ala Val Thr Phe Asn Val Asn Ala Gln Thr Val Trp Gly Glu 
465                 470                 475                 480 


Asn Ile Tyr Ile Thr Gly Ser Val Asp Ala Leu Ser Asn Trp Ser Pro 
                485                 490                 495     


Asp Asn Ala Leu Leu Leu Ser Ser Ala Asn Tyr Pro Thr Trp Ser Ile 
            500                 505                 510         


Thr Val Asn Leu Pro Ala Ser Thr Ala Ile Gln Tyr Lys Tyr Ile Arg 
        515                 520                 525             


Lys Asn Asn Gly Ala Val Thr Trp Glu Ser Asp Pro Asn Asn Ser Ile 
    530                 535                 540                 


Thr Thr Pro Ala Ser Gly Ser Val Thr Glu Asn Asp Thr Trp Arg 
545                 550                 555                 


<210>  8
<211>  583
<212>  PRT
<213>  Artificial

<220>
<223>  Hybrid alpha-amylase comprising catalytic domain from Rhizomucor 
       pusillus alpha-amylase fused to linker and starch binding domain 
       from Aspergillus niger glucoamylase

<400>  8

Ala Thr Ser Asp Asp Trp Lys Gly Lys Ala Ile Tyr Gln Leu Leu Thr 
1               5                   10                  15      


Asp Arg Phe Gly Arg Ala Asp Asp Ser Thr Ser Asn Cys Ser Asn Leu 
            20                  25                  30          


Ser Asn Tyr Cys Gly Gly Thr Tyr Glu Gly Ile Thr Lys His Leu Asp 
        35                  40                  45              


Tyr Ile Ser Gly Met Gly Phe Asp Ala Ile Trp Ile Ser Pro Ile Pro 
    50                  55                  60                  


Lys Asn Ser Asp Gly Gly Tyr His Gly Tyr Trp Ala Thr Asp Phe Tyr 
65                  70                  75                  80  


Gln Leu Asn Ser Asn Phe Gly Asp Glu Ser Gln Leu Lys Ala Leu Ile 
                85                  90                  95      


Gln Ala Ala His Glu Arg Asp Met Tyr Val Met Leu Asp Val Val Ala 
            100                 105                 110         


Asn His Ala Gly Pro Thr Ser Asn Gly Tyr Ser Gly Tyr Thr Phe Gly 
        115                 120                 125             


Asp Ala Ser Leu Tyr His Pro Lys Cys Thr Ile Asp Tyr Asn Asp Gln 
    130                 135                 140                 


Thr Ser Ile Glu Gln Cys Trp Val Ala Asp Glu Leu Pro Asp Ile Asp 
145                 150                 155                 160 


Thr Glu Asn Ser Asp Asn Val Ala Ile Leu Asn Asp Ile Val Ser Gly 
                165                 170                 175     


Trp Val Gly Asn Tyr Ser Phe Asp Gly Ile Arg Ile Asp Thr Val Lys 
            180                 185                 190         


His Ile Arg Lys Asp Phe Trp Thr Gly Tyr Ala Glu Ala Ala Gly Val 
        195                 200                 205             


Phe Ala Thr Gly Glu Val Phe Asn Gly Asp Pro Ala Tyr Val Gly Pro 
    210                 215                 220                 


Tyr Gln Lys Tyr Leu Pro Ser Leu Ile Asn Tyr Pro Met Tyr Tyr Ala 
225                 230                 235                 240 


Leu Asn Asp Val Phe Val Ser Lys Ser Lys Gly Phe Ser Arg Ile Ser 
                245                 250                 255     


Glu Met Leu Gly Ser Asn Arg Asn Ala Phe Glu Asp Thr Ser Val Leu 
            260                 265                 270         


Thr Thr Phe Val Asp Asn His Asp Asn Pro Arg Phe Leu Asn Ser Gln 
        275                 280                 285             


Ser Asp Lys Ala Leu Phe Lys Asn Ala Leu Thr Tyr Val Leu Leu Gly 
    290                 295                 300                 


Glu Gly Ile Pro Ile Val Tyr Tyr Gly Ser Glu Gln Gly Phe Ser Gly 
305                 310                 315                 320 


Gly Ala Asp Pro Ala Asn Arg Glu Val Leu Trp Thr Thr Asn Tyr Asp 
                325                 330                 335     


Thr Ser Ser Asp Leu Tyr Gln Phe Ile Lys Thr Val Asn Ser Val Arg 
            340                 345                 350         


Met Lys Ser Asn Lys Ala Val Tyr Met Asp Ile Tyr Val Gly Asp Asn 
        355                 360                 365             


Ala Tyr Ala Phe Lys His Gly Asp Ala Leu Val Val Leu Asn Asn Tyr 
    370                 375                 380                 


Gly Ser Gly Ser Thr Asn Gln Val Ser Phe Ser Val Ser Gly Lys Phe 
385                 390                 395                 400 


Asp Ser Gly Ala Ser Leu Met Asp Ile Val Ser Asn Ile Thr Thr Thr 
                405                 410                 415     


Val Ser Ser Asp Gly Thr Val Thr Phe Asn Leu Lys Asp Gly Leu Pro 
            420                 425                 430         


Ala Ile Phe Thr Ser Ala Thr Gly Gly Thr Thr Thr Thr Ala Thr Pro 
        435                 440                 445             


Thr Gly Ser Gly Ser Val Thr Ser Thr Ser Lys Thr Thr Ala Thr Ala 
    450                 455                 460                 


Ser Lys Thr Ser Thr Ser Thr Ser Ser Thr Ser Cys Thr Thr Pro Thr 
465                 470                 475                 480 


Ala Val Ala Val Thr Phe Asp Leu Thr Ala Thr Thr Thr Tyr Gly Glu 
                485                 490                 495     


Asn Ile Tyr Leu Val Gly Ser Ile Ser Gln Leu Gly Asp Trp Glu Thr 
            500                 505                 510         


Ser Asp Gly Ile Ala Leu Ser Ala Asp Lys Tyr Thr Ser Ser Asp Pro 
        515                 520                 525             


Leu Trp Tyr Val Thr Val Thr Leu Pro Ala Gly Glu Ser Phe Glu Tyr 
    530                 535                 540                 


Lys Phe Ile Arg Ile Glu Ser Asp Asp Ser Val Glu Trp Glu Ser Asp 
545                 550                 555                 560 


Pro Asn Arg Glu Tyr Thr Val Pro Gln Ala Cys Gly Thr Ser Thr Ala 
                565                 570                 575     


Thr Val Thr Asp Thr Trp Arg 
            580             


<210>  9
<211>  424
<212>  PRT
<213>  Thermococcus litoralis

<400>  9

Met Glu Phe Asn Lys Val Phe Ser Leu Leu Leu Val Phe Val Val Leu 
1               5                   10                  15      


Gly Ala Thr Ala Gly Ile Val Gly Ala Val Ser Ala Glu Lys Val Arg 
            20                  25                  30          


Val Ile Ile Thr Ile Asp Lys Asp Phe Asn Glu Asn Ser Val Phe Ala 
        35                  40                  45              


Leu Gly Gly Asn Val Val Ala Arg Gly Lys Val Phe Pro Ile Val Ile 
    50                  55                  60                  


Ala Glu Leu Ser Pro Arg Ala Val Glu Arg Leu Lys Asn Ala Lys Gly 
65                  70                  75                  80  


Val Val Arg Val Glu Tyr Asp Ala Glu Val Gln Val Leu Lys Gly Lys 
                85                  90                  95      


Ser Pro Gly Ala Gly Lys Pro Lys Pro Ser Gln Pro Ala Gln Thr Ile 
            100                 105                 110         


Pro Trp Gly Ile Glu Arg Ile Lys Ala Pro Asp Val Trp Ser Ile Thr 
        115                 120                 125             


Asp Gly Ser Ser Ser Gly Val Ile Glu Val Ala Ile Leu Asp Thr Gly 
    130                 135                 140                 


Ile Asp Tyr Asp His Pro Asp Leu Ala Ala Asn Leu Ala Trp Gly Val 
145                 150                 155                 160 


Ser Val Leu Arg Gly Lys Val Ser Thr Lys Pro Lys Asp Tyr Lys Asp 
                165                 170                 175     


Gln Asn Gly His Gly Thr His Val Ala Gly Thr Val Ala Ala Leu Asn 
            180                 185                 190         


Asn Asp Ile Gly Val Val Gly Val Ala Pro Ala Val Glu Ile Tyr Ala 
        195                 200                 205             


Val Arg Val Leu Asp Ala Ser Gly Arg Gly Ser Tyr Ser Asp Ile Ile 
    210                 215                 220                 


Leu Gly Ile Glu Gln Ala Leu Leu Gly Pro Asp Gly Val Leu Asp Ser 
225                 230                 235                 240 


Asp Gly Asp Gly Ile Ile Val Gly Asp Pro Asp Asp Asp Ala Ala Glu 
                245                 250                 255     


Val Ile Ser Met Ser Leu Gly Gly Leu Ser Asp Val Gln Ala Phe His 
            260                 265                 270         


Asp Ala Ile Ile Glu Ala Tyr Asn Tyr Gly Val Val Ile Val Ala Ala 
        275                 280                 285             


Ser Gly Asn Glu Gly Ala Ser Ser Pro Ser Tyr Pro Ala Ala Tyr Pro 
    290                 295                 300                 


Glu Val Ile Ala Val Gly Ala Thr Asp Val Asn Asp Gln Val Pro Trp 
305                 310                 315                 320 


Trp Ser Asn Arg Gly Val Glu Val Ser Ala Pro Gly Val Asp Val Leu 
                325                 330                 335     


Ser Thr Tyr Pro Asp Asp Ser Tyr Glu Thr Leu Ser Gly Thr Ser Met 
            340                 345                 350         


Ala Thr Pro His Val Ser Gly Val Val Ala Leu Ile Gln Ala Ala Tyr 
        355                 360                 365             


Tyr Asn Lys Tyr Gly Ser Val Leu Pro Val Gly Thr Phe Asp Asp Asn 
    370                 375                 380                 


Thr Met Ser Thr Val Arg Gly Ile Leu His Ile Thr Ala Asp Asp Leu 
385                 390                 395                 400 


Gly Ser Ser Gly Trp Asp Ala Asp Tyr Gly Tyr Gly Ile Val Arg Ala 
                405                 410                 415     


Asp Leu Ala Val Gln Ala Val Asn 
            420                 


<210>  10
<211>  422
<212>  PRT
<213>  Thermococcus thioreducens

<400>  10

Met Gly Arg Lys Asp Ile Thr Ile Ala Leu Val Ala Leu Ile Val Leu 
1               5                   10                  15      


Ser Leu Leu Gly Val Pro Ala Thr Ala Glu Lys Pro Glu Leu Val Arg 
            20                  25                  30          


Val Ile Val His Val Asp Arg Gly His Phe Asn Thr Ala Asp Val Ala 
        35                  40                  45              


Thr Ile Gly Gly His Val Val Tyr Gln Phe Lys Leu Ile Asp Ala Val 
    50                  55                  60                  


Val Val Glu Val Pro Ser Thr Ala Val Gly Arg Leu Lys Lys Leu Pro 
65                  70                  75                  80  


Gly Val Lys Met Val Glu Phe Asp His Lys Ala Arg Ile Leu Ala Gly 
                85                  90                  95      


Pro Pro Ser Trp Leu Gly Gly Gly Gln Pro Ser Gln Gln Ile Pro Trp 
            100                 105                 110         


Gly Ile Ser Arg Val Arg Ala Pro Asp Val Trp Gly Ile Thr Asp Gly 
        115                 120                 125             


Ser Gly Gly Val Ile Glu Val Ala Val Leu Asp Thr Gly Val Asp Tyr 
    130                 135                 140                 


Asp His Pro Asp Leu Ala Gly Asn Ile Ala Trp Cys Val Ser Thr Leu 
145                 150                 155                 160 


Arg Gly Arg Val Thr Thr Asn Pro Ala Gln Cys Lys Asp Gln Asn Gly 
                165                 170                 175     


His Gly Thr His Val Ile Gly Thr Ile Ala Ala Leu Asn Asn Asp Ile 
            180                 185                 190         


Gly Val Val Gly Val Ala Pro Gly Val Glu Ile Tyr Ser Ile Arg Val 
        195                 200                 205             


Leu Asp Ala Ser Gly Ser Gly Ser Tyr Ser Asp Ile Ala Ile Gly Ile 
    210                 215                 220                 


Glu Gln Ala Leu Leu Gly Pro Asp Gly Ile Leu Asp Lys Asp Gly Asp 
225                 230                 235                 240 


Gly Ile Ile Val Gly Asp Pro Asp Asp Asp Ala Ala Glu Val Ile Ser 
                245                 250                 255     


Met Ser Leu Gly Gly Pro Thr Asp Asp Gln Tyr Leu His Asp Met Ile 
            260                 265                 270         


Ile Thr Ala Tyr Asn Tyr Gly Val Val Ile Val Ala Ala Ser Gly Asn 
        275                 280                 285             


Glu Gly Ala Ser Ser Pro Ser Tyr Pro Ala Ala Tyr Pro Glu Val Ile 
    290                 295                 300                 


Ala Val Gly Ala Ser Asp Val Asn Asp Gln Ile Ala Ser Trp Ser Asn 
305                 310                 315                 320 


Arg Gln Pro Glu Val Ser Ala Pro Gly Val Asp Ile Leu Ser Thr Tyr 
                325                 330                 335     


Pro Asp Asp Thr Tyr Glu Thr Leu Ser Gly Thr Ser Met Ala Thr Pro 
            340                 345                 350         


His Val Ser Gly Val Val Ala Leu Ile Gln Ala Ala Tyr Tyr Asn Lys 
        355                 360                 365             


Tyr Gly Lys Val Leu Pro Val Gly Thr Phe Asp Asp Met Gly Thr Asn 
    370                 375                 380                 


Thr Val Arg Gly Ile Leu His Val Thr Ala Asp Asp Leu Gly Asp Ala 
385                 390                 395                 400 


Gly Trp Asp Ile Tyr Tyr Gly Tyr Gly Ile Val Arg Ala Asp Leu Ala 
                405                 410                 415     


Val Gln Ala Ala Ile Gly 
            420         


<210>  11
<211>  595
<212>  PRT
<213>  Penicillium oxalicum

<400>  11

Arg Pro Asp Pro Lys Gly Gly Asn Leu Thr Pro Phe Ile His Lys Glu 
1               5                   10                  15      


Gly Glu Arg Ser Leu Gln Gly Ile Leu Asp Asn Leu Gly Gly Arg Gly 
            20                  25                  30          


Lys Lys Thr Pro Gly Thr Ala Ala Gly Leu Phe Ile Ala Ser Pro Asn 
        35                  40                  45              


Thr Glu Asn Pro Asn Tyr Tyr Tyr Thr Trp Thr Arg Asp Ser Ala Leu 
    50                  55                  60                  


Thr Ala Lys Cys Leu Ile Asp Leu Phe Glu Asp Ser Arg Ala Lys Phe 
65                  70                  75                  80  


Pro Ile Asp Arg Lys Tyr Leu Glu Thr Gly Ile Arg Asp Tyr Lys Ser 
                85                  90                  95      


Ser Gln Ala Ile Leu Gln Ser Val Ser Asn Pro Ser Gly Thr Leu Lys 
            100                 105                 110         


Asp Gly Ser Gly Leu Gly Glu Pro Lys Phe Glu Ile Asp Leu Asn Pro 
        115                 120                 125             


Phe Ser Gly Ala Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg 
    130                 135                 140                 


Ala Thr Ala Met Ile Thr Tyr Ala Asn Tyr Leu Ile Ser His Gly Gln 
145                 150                 155                 160 


Lys Ser Asp Val Ser Gln Val Met Trp Pro Ile Ile Ala Asn Asp Leu 
                165                 170                 175     


Ala Tyr Val Gly Gln Tyr Trp Asn Asn Thr Gly Phe Asp Leu Trp Glu 
            180                 185                 190         


Glu Val Asp Gly Ser Ser Phe Phe Thr Ile Ala Val Gln His Arg Ala 
        195                 200                 205             


Leu Val Glu Gly Ser Gln Leu Ala Lys Lys Leu Gly Lys Ser Cys Asp 
    210                 215                 220                 


Ala Cys Asp Ser Gln Pro Pro Gln Ile Leu Cys Phe Leu Gln Ser Phe 
225                 230                 235                 240 


Trp Asn Gly Lys Tyr Ile Thr Ser Asn Ile Asn Thr Gln Ala Ser Arg 
                245                 250                 255     


Ser Gly Ile Asp Leu Asp Ser Val Leu Gly Ser Ile His Thr Phe Asp 
            260                 265                 270         


Pro Glu Ala Ala Cys Asp Asp Ala Thr Phe Gln Pro Cys Ser Ala Arg 
        275                 280                 285             


Ala Leu Ala Asn His Lys Val Tyr Val Asp Ser Phe Arg Ser Ile Tyr 
    290                 295                 300                 


Lys Ile Asn Ala Gly Leu Ala Glu Gly Ser Ala Ala Asn Val Gly Arg 
305                 310                 315                 320 


Tyr Pro Glu Asp Val Tyr Gln Gly Gly Asn Pro Trp Tyr Leu Ala Thr 
                325                 330                 335     


Leu Gly Ala Ser Glu Leu Leu Tyr Asp Ala Leu Tyr Gln Trp Asp Arg 
            340                 345                 350         


Leu Gly Lys Leu Glu Val Ser Glu Thr Ser Leu Ser Phe Phe Lys Asp 
        355                 360                 365             


Phe Asp Ala Thr Val Lys Ile Gly Ser Tyr Ser Arg Asn Ser Lys Thr 
    370                 375                 380                 


Tyr Lys Lys Leu Thr Gln Ser Ile Lys Ser Tyr Ala Asp Gly Phe Ile 
385                 390                 395                 400 


Gln Leu Val Gln Gln Tyr Thr Pro Ser Asn Gly Ser Leu Ala Glu Gln 
                405                 410                 415     


Tyr Asp Arg Asn Thr Ala Ala Pro Leu Ser Ala Asn Asp Leu Thr Trp 
            420                 425                 430         


Ser Phe Ala Ser Phe Leu Thr Ala Thr Gln Arg Arg Asp Ala Val Val 
        435                 440                 445             


Pro Pro Ser Trp Gly Ala Lys Ser Ala Asn Lys Val Pro Thr Thr Cys 
    450                 455                 460                 


Ser Ala Ser Pro Val Val Gly Thr Tyr Lys Ala Pro Thr Ala Thr Phe 
465                 470                 475                 480 


Ser Ser Lys Thr Lys Cys Val Pro Ala Lys Asp Ile Val Pro Ile Thr 
                485                 490                 495     


Phe Tyr Leu Ile Glu Asn Thr Tyr Tyr Gly Glu Asn Val Phe Met Ser 
            500                 505                 510         


Gly Asn Ile Thr Ala Leu Gly Asn Trp Asp Ala Lys Lys Gly Phe Pro 
        515                 520                 525             


Leu Thr Ala Asn Leu Tyr Thr Gln Asp Gln Asn Leu Trp Phe Ala Ser 
    530                 535                 540                 


Val Glu Phe Ile Pro Ala Gly Thr Pro Phe Glu Tyr Lys Tyr Tyr Lys 
545                 550                 555                 560 


Val Glu Pro Asn Gly Asp Ile Thr Trp Glu Lys Gly Pro Asn Arg Val 
                565                 570                 575     


Phe Val Ala Pro Thr Gly Cys Pro Val Gln Pro His Ser Asn Asp Val 
            580                 585                 590         


Trp Gln Phe 
        595 


