                         SEQUENCE LISTING

<110>  Fred Hutchinson Cancer Research Center
       Dudakov, Jarrod
       Kiem, Hans-Peter
       Van Den Brink, Marcel
       Radtke, Stephan
       Velardi, Enrico
       James, Scott
 
<120>  LUTEINIZING HORMONE RECEPTOR BINDING AGENTS AND LUTEINIZING 
       HORMONE AGONISTS TO IDENTIFY, EXPAND, ABLATE AND MODIFY PRIMITIVE
        STEM CELL POPULATIONS

<130>  F053-0069PCT/18-002-WO-PCT

<140>  PCT/US2018/054004
<141>  2018-10-02

<150>  US 62/566,897
<151>  2017-10-02

<160>  90    

<170>  PatentIn version 3.5

<210>  1
<211>  699
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Lys Gln Arg Phe Ser Ala Leu Gln Leu Leu Lys Leu Leu Leu Leu 
1               5                   10                  15      


Leu Gln Pro Pro Leu Pro Arg Ala Leu Arg Glu Ala Leu Cys Pro Glu 
            20                  25                  30          


Pro Cys Asn Cys Val Pro Asp Gly Ala Leu Arg Cys Pro Gly Pro Thr 
        35                  40                  45              


Ala Gly Leu Thr Arg Leu Ser Leu Ala Tyr Leu Pro Val Lys Val Ile 
    50                  55                  60                  


Pro Ser Gln Ala Phe Arg Gly Leu Asn Glu Val Ile Lys Ile Glu Ile 
65                  70                  75                  80  


Ser Gln Ile Asp Ser Leu Glu Arg Ile Glu Ala Asn Ala Phe Asp Asn 
                85                  90                  95      


Leu Leu Asn Leu Ser Glu Ile Leu Ile Gln Asn Thr Lys Asn Leu Arg 
            100                 105                 110         


Tyr Ile Glu Pro Gly Ala Phe Ile Asn Leu Pro Arg Leu Lys Tyr Leu 
        115                 120                 125             


Ser Ile Cys Asn Thr Gly Ile Arg Lys Phe Pro Asp Val Thr Lys Val 
    130                 135                 140                 


Phe Ser Ser Glu Ser Asn Phe Ile Leu Glu Ile Cys Asp Asn Leu His 
145                 150                 155                 160 


Ile Thr Thr Ile Pro Gly Asn Ala Phe Gln Gly Met Asn Asn Glu Ser 
                165                 170                 175     


Val Thr Leu Lys Leu Tyr Gly Asn Gly Phe Glu Glu Val Gln Ser His 
            180                 185                 190         


Ala Phe Asn Gly Thr Thr Leu Thr Ser Leu Glu Leu Lys Glu Asn Val 
        195                 200                 205             


His Leu Glu Lys Met His Asn Gly Ala Phe Arg Gly Ala Thr Gly Pro 
    210                 215                 220                 


Lys Thr Leu Asp Ile Ser Ser Thr Lys Leu Gln Ala Leu Pro Ser Tyr 
225                 230                 235                 240 


Gly Leu Glu Ser Ile Gln Arg Leu Ile Ala Thr Ser Ser Tyr Ser Leu 
                245                 250                 255     


Lys Lys Leu Pro Ser Arg Glu Thr Phe Val Asn Leu Leu Glu Ala Thr 
            260                 265                 270         


Leu Thr Tyr Pro Ser His Cys Cys Ala Phe Arg Asn Leu Pro Thr Lys 
        275                 280                 285             


Glu Gln Asn Phe Ser His Ser Ile Ser Glu Asn Phe Ser Lys Gln Cys 
    290                 295                 300                 


Glu Ser Thr Val Arg Lys Val Asn Asn Lys Thr Leu Tyr Ser Ser Met 
305                 310                 315                 320 


Leu Ala Glu Ser Glu Leu Ser Gly Trp Asp Tyr Glu Tyr Gly Phe Cys 
                325                 330                 335     


Leu Pro Lys Thr Pro Arg Cys Ala Pro Glu Pro Asp Ala Phe Asn Pro 
            340                 345                 350         


Cys Glu Asp Ile Met Gly Tyr Asp Phe Leu Arg Val Leu Ile Trp Leu 
        355                 360                 365             


Ile Asn Ile Leu Ala Ile Met Gly Asn Met Thr Val Leu Phe Val Leu 
    370                 375                 380                 


Leu Thr Ser Arg Tyr Lys Leu Thr Val Pro Arg Phe Leu Met Cys Asn 
385                 390                 395                 400 


Leu Ser Phe Ala Asp Phe Cys Met Gly Leu Tyr Leu Leu Leu Ile Ala 
                405                 410                 415     


Ser Val Asp Ser Gln Thr Lys Gly Gln Tyr Tyr Asn His Ala Ile Asp 
            420                 425                 430         


Trp Gln Thr Gly Ser Gly Cys Ser Thr Ala Gly Phe Phe Thr Val Phe 
        435                 440                 445             


Ala Ser Glu Leu Ser Val Tyr Thr Leu Thr Val Ile Thr Leu Glu Arg 
    450                 455                 460                 


Trp His Thr Ile Thr Tyr Ala Ile His Leu Asp Gln Lys Leu Arg Leu 
465                 470                 475                 480 


Arg His Ala Ile Leu Ile Met Leu Gly Gly Trp Leu Phe Ser Ser Leu 
                485                 490                 495     


Ile Ala Met Leu Pro Leu Val Gly Val Ser Asn Tyr Met Lys Val Ser 
            500                 505                 510         


Ile Cys Phe Pro Met Asp Val Glu Thr Thr Leu Ser Gln Val Tyr Ile 
        515                 520                 525             


Leu Thr Ile Leu Ile Leu Asn Val Val Ala Phe Phe Ile Ile Cys Ala 
    530                 535                 540                 


Cys Tyr Ile Lys Ile Tyr Phe Ala Val Arg Asn Pro Glu Leu Met Ala 
545                 550                 555                 560 


Thr Asn Lys Asp Thr Lys Ile Ala Lys Lys Met Ala Ile Leu Ile Phe 
                565                 570                 575     


Thr Asp Phe Thr Cys Met Ala Pro Ile Ser Phe Phe Ala Ile Ser Ala 
            580                 585                 590         


Ala Phe Lys Val Pro Leu Ile Thr Val Thr Asn Ser Lys Val Leu Leu 
        595                 600                 605             


Val Leu Phe Tyr Pro Ile Asn Ser Cys Ala Asn Pro Phe Leu Tyr Ala 
    610                 615                 620                 


Ile Phe Thr Lys Thr Phe Gln Arg Asp Phe Phe Leu Leu Leu Ser Lys 
625                 630                 635                 640 


Phe Gly Cys Cys Lys Arg Arg Ala Glu Leu Tyr Arg Arg Lys Asp Phe 
                645                 650                 655     


Ser Ala Tyr Thr Ser Asn Cys Lys Asn Gly Phe Thr Gly Ser Asn Lys 
            660                 665                 670         


Pro Ser Gln Ser Thr Leu Lys Leu Ser Thr Leu His Cys Gln Gly Thr 
        675                 680                 685             


Ala Leu Leu Asp Lys Thr Arg Tyr Thr Glu Cys 
    690                 695                 


<210>  2
<211>  700
<212>  PRT
<213>  Mus musculus

<400>  2

Met Gly Arg Arg Val Pro Ala Leu Arg Gln Leu Leu Val Leu Ala Met 
1               5                   10                  15      


Leu Val Leu Lys Gln Ser Gln Leu His Ser Pro Glu Leu Ser Gly Ser 
            20                  25                  30          


Arg Cys Pro Glu Pro Cys Asp Cys Ala Pro Asp Gly Ala Leu Arg Cys 
        35                  40                  45              


Pro Gly Pro Arg Ala Gly Leu Ala Arg Leu Ser Leu Thr Tyr Leu Pro 
    50                  55                  60                  


Val Lys Val Ile Pro Ser Gln Ala Phe Arg Gly Leu Asn Glu Val Val 
65                  70                  75                  80  


Lys Ile Glu Ile Ser Gln Ser Asp Ser Leu Glu Arg Ile Glu Ala Asn 
                85                  90                  95      


Ala Phe Asp Asn Leu Leu Asn Leu Ser Glu Ile Leu Ile Gln Asn Thr 
            100                 105                 110         


Lys Asn Leu Leu Tyr Ile Glu Pro Gly Ala Phe Thr Asn Leu Pro Arg 
        115                 120                 125             


Leu Lys Tyr Leu Ser Ile Cys Asn Thr Gly Ile Arg Thr Leu Pro Asp 
    130                 135                 140                 


Val Ser Lys Ile Ser Ser Ser Glu Phe Asn Phe Ile Leu Glu Ile Cys 
145                 150                 155                 160 


Asp Asn Leu Tyr Ile Thr Thr Ile Pro Gly Asn Ala Phe Gln Gly Met 
                165                 170                 175     


Asn Asn Glu Ser Ile Thr Leu Lys Leu Tyr Gly Asn Gly Phe Glu Glu 
            180                 185                 190         


Val Gln Ser His Ala Phe Asn Gly Thr Thr Leu Ile Ser Leu Glu Leu 
        195                 200                 205             


Lys Glu Asn Ile Tyr Leu Glu Lys Met His Ser Gly Thr Phe Gln Gly 
    210                 215                 220                 


Ala Thr Gly Pro Ser Ile Leu Asp Val Ser Ser Thr Lys Leu Gln Ala 
225                 230                 235                 240 


Leu Pro Ser His Gly Leu Glu Ser Ile Gln Thr Leu Ile Ala Thr Ser 
                245                 250                 255     


Ser Tyr Ser Leu Lys Thr Leu Pro Ser Arg Glu Lys Phe Thr Ser Leu 
            260                 265                 270         


Leu Val Ala Thr Leu Thr Tyr Pro Ser His Cys Cys Ala Phe Arg Asn 
        275                 280                 285             


Leu Pro Lys Lys Glu Gln Asn Phe Ser Phe Ser Ile Phe Glu Asn Phe 
    290                 295                 300                 


Ser Lys Gln Cys Glu Ser Thr Val Arg Glu Ala Asn Asn Glu Thr Leu 
305                 310                 315                 320 


Tyr Ser Ala Ile Phe Glu Glu Asn Glu Leu Ser Gly Trp Asp Tyr Asp 
                325                 330                 335     


Tyr Asp Phe Cys Ser Pro Lys Thr Leu Gln Cys Thr Pro Glu Pro Asp 
            340                 345                 350         


Ala Phe Asn Pro Cys Glu Asp Ile Met Gly Tyr Ala Phe Leu Arg Val 
        355                 360                 365             


Leu Ile Trp Leu Ile Asn Ile Leu Ala Ile Phe Gly Asn Leu Thr Val 
    370                 375                 380                 


Leu Phe Val Leu Leu Thr Ser Arg Tyr Lys Leu Thr Val Pro Arg Phe 
385                 390                 395                 400 


Leu Met Cys Asn Leu Ser Phe Ala Asp Phe Cys Met Gly Leu Tyr Leu 
                405                 410                 415     


Leu Leu Ile Ala Ser Val Asp Ser Gln Thr Lys Gly Gln Tyr Tyr Asn 
            420                 425                 430         


His Ala Ile Asp Trp Gln Thr Gly Ser Gly Cys Ser Ala Ala Gly Phe 
        435                 440                 445             


Phe Thr Val Phe Ala Ser Glu Leu Ser Val Tyr Thr Leu Thr Val Ile 
    450                 455                 460                 


Thr Leu Glu Arg Trp His Thr Ile Thr Tyr Ala Val Gln Leu Asp Gln 
465                 470                 475                 480 


Lys Leu Arg Leu Arg His Ala Ile Pro Ile Met Leu Gly Gly Trp Ile 
                485                 490                 495     


Phe Ser Thr Leu Met Ala Thr Leu Pro Leu Val Gly Val Ser Ser Tyr 
            500                 505                 510         


Met Lys Val Ser Ile Cys Leu Pro Met Asp Val Glu Ser Thr Leu Ser 
        515                 520                 525             


Gln Val Tyr Ile Leu Ser Ile Leu Leu Leu Asn Ala Val Ala Phe Val 
    530                 535                 540                 


Val Ile Cys Ala Cys Tyr Val Arg Ile Tyr Phe Ala Val Gln Asn Pro 
545                 550                 555                 560 


Glu Leu Thr Ala Pro Asn Lys Asp Thr Lys Ile Ala Lys Lys Met Ala 
                565                 570                 575     


Ile Leu Ile Phe Thr Asp Phe Thr Cys Met Ala Pro Ile Ser Phe Phe 
            580                 585                 590         


Ala Ile Ser Ala Ala Phe Lys Val Pro Leu Ile Thr Val Thr Asn Ser 
        595                 600                 605             


Lys Val Leu Leu Val Leu Phe Tyr Pro Val Asn Ser Cys Ala Asn Pro 
    610                 615                 620                 


Phe Leu Tyr Ala Val Phe Thr Lys Ala Phe Gln Arg Asp Phe Phe Leu 
625                 630                 635                 640 


Leu Leu Ser Arg Phe Gly Cys Cys Lys His Arg Ala Glu Leu Tyr Arg 
                645                 650                 655     


Arg Lys Glu Phe Ser Ala Cys Thr Phe Asn Ser Lys Asn Gly Phe Pro 
            660                 665                 670         


Arg Ser Ser Lys Pro Ser Gln Ala Ala Leu Lys Leu Ser Ile Val His 
        675                 680                 685             


Cys Gln Gln Pro Thr Pro Pro Arg Val Leu Ile Gln 
    690                 695                 700 


<210>  3
<211>  700
<212>  PRT
<213>  Rattus norvegicus

<400>  3

Met Gly Arg Arg Val Pro Ala Leu Arg Gln Leu Leu Val Leu Ala Val 
1               5                   10                  15      


Leu Leu Leu Lys Pro Ser Gln Leu Gln Ser Arg Glu Leu Ser Gly Ser 
            20                  25                  30          


Arg Cys Pro Glu Pro Cys Asp Cys Ala Pro Asp Gly Ala Leu Arg Cys 
        35                  40                  45              


Pro Gly Pro Arg Ala Gly Leu Ala Arg Leu Ser Leu Thr Tyr Leu Pro 
    50                  55                  60                  


Val Lys Val Ile Pro Ser Gln Ala Phe Arg Gly Leu Asn Glu Val Val 
65                  70                  75                  80  


Lys Ile Glu Ile Ser Gln Ser Asp Ser Leu Glu Arg Ile Glu Ala Asn 
                85                  90                  95      


Ala Phe Asp Asn Leu Leu Asn Leu Ser Glu Leu Leu Ile Gln Asn Thr 
            100                 105                 110         


Lys Asn Leu Leu Tyr Ile Glu Pro Gly Ala Phe Thr Asn Leu Pro Arg 
        115                 120                 125             


Leu Lys Tyr Leu Ser Ile Cys Asn Thr Gly Ile Arg Thr Leu Pro Asp 
    130                 135                 140                 


Val Thr Lys Ile Ser Ser Ser Glu Phe Asn Phe Ile Leu Glu Ile Cys 
145                 150                 155                 160 


Asp Asn Leu His Ile Thr Thr Ile Pro Gly Asn Ala Phe Gln Gly Met 
                165                 170                 175     


Asn Asn Glu Ser Val Thr Leu Lys Leu Tyr Gly Asn Gly Phe Glu Glu 
            180                 185                 190         


Val Gln Ser His Ala Phe Asn Gly Thr Thr Leu Ile Ser Leu Glu Leu 
        195                 200                 205             


Lys Glu Asn Ile Tyr Leu Glu Lys Met His Ser Gly Ala Phe Gln Gly 
    210                 215                 220                 


Ala Thr Gly Pro Ser Ile Leu Asp Ile Ser Ser Thr Lys Leu Gln Ala 
225                 230                 235                 240 


Leu Pro Ser His Gly Leu Glu Ser Ile Gln Thr Leu Ile Ala Leu Ser 
                245                 250                 255     


Ser Tyr Ser Leu Lys Thr Leu Pro Ser Lys Glu Lys Phe Thr Ser Leu 
            260                 265                 270         


Leu Val Ala Thr Leu Thr Tyr Pro Ser His Cys Cys Ala Phe Arg Asn 
        275                 280                 285             


Leu Pro Lys Lys Glu Gln Asn Phe Ser Phe Ser Ile Phe Glu Asn Phe 
    290                 295                 300                 


Ser Lys Gln Cys Glu Ser Thr Val Arg Lys Ala Asp Asn Glu Thr Leu 
305                 310                 315                 320 


Tyr Ser Ala Ile Phe Glu Glu Asn Glu Leu Ser Gly Trp Asp Tyr Asp 
                325                 330                 335     


Tyr Gly Phe Cys Ser Pro Lys Thr Leu Gln Cys Ala Pro Glu Pro Asp 
            340                 345                 350         


Ala Phe Asn Pro Cys Glu Asp Ile Met Gly Tyr Ala Phe Leu Arg Val 
        355                 360                 365             


Leu Ile Trp Leu Ile Asn Ile Leu Ala Ile Phe Gly Asn Leu Thr Val 
    370                 375                 380                 


Leu Phe Val Leu Leu Thr Ser Arg Tyr Lys Leu Thr Val Pro Arg Phe 
385                 390                 395                 400 


Leu Met Cys Asn Leu Ser Phe Ala Asp Phe Cys Met Gly Leu Tyr Leu 
                405                 410                 415     


Leu Leu Ile Ala Ser Val Asp Ser Gln Thr Lys Gly Gln Tyr Tyr Asn 
            420                 425                 430         


His Ala Ile Asp Trp Gln Thr Gly Ser Gly Cys Gly Ala Ala Gly Phe 
        435                 440                 445             


Phe Thr Val Phe Ala Ser Glu Leu Ser Val Tyr Thr Leu Thr Val Ile 
    450                 455                 460                 


Thr Leu Glu Arg Trp His Thr Ile Thr Tyr Ala Val Gln Leu Asp Gln 
465                 470                 475                 480 


Lys Leu Arg Leu Arg His Ala Ile Pro Ile Met Leu Gly Gly Trp Leu 
                485                 490                 495     


Phe Ser Thr Leu Ile Ala Thr Met Pro Leu Val Gly Ile Ser Asn Tyr 
            500                 505                 510         


Met Lys Val Ser Ile Cys Leu Pro Met Asp Val Glu Ser Thr Leu Ser 
        515                 520                 525             


Gln Val Tyr Ile Leu Ser Ile Leu Ile Leu Asn Val Val Ala Phe Val 
    530                 535                 540                 


Val Ile Cys Ala Cys Tyr Ile Arg Ile Tyr Phe Ala Val Gln Asn Pro 
545                 550                 555                 560 


Glu Leu Thr Ala Pro Asn Lys Asp Thr Lys Ile Ala Lys Lys Met Ala 
                565                 570                 575     


Ile Leu Ile Phe Thr Asp Phe Thr Cys Met Ala Pro Ile Ser Phe Phe 
            580                 585                 590         


Ala Ile Ser Ala Ala Phe Lys Val Pro Leu Ile Thr Val Thr Asn Ser 
        595                 600                 605             


Lys Ile Leu Leu Val Leu Phe Tyr Pro Val Asn Ser Cys Ala Asn Pro 
    610                 615                 620                 


Phe Leu Tyr Ala Ile Phe Thr Lys Ala Phe Gln Arg Asp Phe Leu Leu 
625                 630                 635                 640 


Leu Leu Ser Arg Phe Gly Cys Cys Lys Arg Arg Ala Glu Leu Tyr Arg 
                645                 650                 655     


Arg Lys Glu Phe Ser Ala Tyr Thr Ser Asn Cys Lys Asn Gly Phe Pro 
            660                 665                 670         


Gly Ala Ser Lys Pro Ser Gln Ala Thr Leu Lys Leu Ser Thr Val His 
        675                 680                 685             


Cys Gln Gln Pro Ile Pro Pro Arg Ala Leu Thr His 
    690                 695                 700 


<210>  4
<211>  462
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Variant of CD3zeta and a portion of the 4-1BB intracellular 
       signaling domain

<400>  4
aaacggggca gaaagaaact cctgtatata ttcaaacaac catttatgag accagtacaa       60

actactcaag aggaagatgg ctgtagctgc cgatttccag aagaagaaga aggaggatgt      120

gaactgcggg tgaagttcag cagaagcgcc gacgcccctg cctaccagca gggccagaat      180

cagctgtaca acgagctgaa cctgggcaga agggaagagt acgacgtcct ggataagcgg      240

agaggccggg accctgagat gggcggcaag cctcggcgga agaaccccca ggaaggcctg      300

tataacgaac tgcagaaaga caagatggcc gaggcctaca gcgagatcgg catgaagggc      360

gagcggaggc ggggcaaggg ccacgacggc ctgtatcagg gcctgtccac cgccaccaag      420

gatacctacg acgccctgca catgcaggcc ctgcccccaa gg                         462


<210>  5
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant of CD3zeta and a portion of the 4-1BB intracellular 
       signaling domain

<400>  5

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg 
        35                  40                  45              


Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn 
    50                  55                  60                  


Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
65                  70                  75                  80  


Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro 
                85                  90                  95      


Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala 
            100                 105                 110         


Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His 
        115                 120                 125             


Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp 
    130                 135                 140                 


Ala Leu His Met Gln Ala Leu Pro Pro Arg 
145                 150                 


<210>  6
<211>  2046
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LH-CAR cDNA

<400>  6
atggcctcac cgttgacccg ctttctgtcg ctgaacctgc tgctgctggg tgagtcgatt       60

atcctgggga gtggagaagc ttccaggggc ccccttcggc cactgtgccg gcctgtcaac      120

gcaactctgg ccgcagagaa tgagttctgc ccagtctgca tcaccttcac caccagcatc      180

tgtgccggct actgtcctag catggtccga gtactgccgg ctgctttgcc tcctgtgcct      240

cagccagtgt gcacctaccg ggagctgcgc ttcgcatctg tccgcctccc tggctgccca      300

ccgggtgtag accccatagt ctcctttcct gtagccctca gctgccgctg tgggccctgc      360

cggctcagta gctctgactg tgggggtccc aggactcaac caatggcctg tgacctcccc      420

cacctccccg gcctcctcct cctcggtgga ggtggatcag gtggaggtgg atctggtgga      480

ggtggatctc ttcctgatgg agactttatt attcagggtt gcccagaatg taaactaaag      540

gaaaataaat acttctccaa gctaggagcc cccatctacc agtgtatggg ctgttgcttc      600

tccagggcat atcccactcc tgccaggtcc aagaagacaa tgctggttcc aaagaatatt      660

acctcggagg ccacatgctg tgtggccaaa gcatttacta aggccacagt aatgggaaat      720

gccagagtgg agaatcatac ggagtgccac tgtagcactt gctactacca caagtcggaa      780

caaaaactca tctcagaaga ggatctggcg gccgcatcta ctactaccaa gccagtgctg      840

cgaactccct cacctgtgca ccctaccggg acatctcagc cccagagacc agaagattgt      900

cggccccgtg gctcagtgaa ggggaccgga ttggacttcg cctgtgatat ttacatctgg      960

gcacccttgg ccggaatctg cgtggccctt ctgctgtcct tgatcatcac tctcatctgc     1020

tacaatagta gaaggaacag actccttcaa agtgactaca tgaacatgac tccccggagg     1080

cctgggctca ctcgaaagcc ttaccagccc tacgcccctg ccagagactt tgcagcgtac     1140

cgccccagag caaaattcag caggagtgca gagactgctg ccaacctgca ggaccccaac     1200

cagctctaca atgagctcaa tctagggcga agagaggaat atgacgtctt ggagaagaag     1260

cgggctcggg atccagagat gggaggcaaa cagcagagga ggaggaaccc ccaggaaggc     1320

gtatacaatg cactgcagaa agacaagatg gcagaagcct acagtgagat cggcacaaaa     1380

ggcgagaggc ggagaggcaa ggggcacgat ggcctttacc agggtctcag cactgccacc     1440

aaggacacct atgatgccct gcatatgcag accctggccc ctcgcggtac cggtcaatgt     1500

actaactacg ctttgttgaa actcgctggc gatgttgaaa gtaaccccgg tcctggatcc     1560

atgaacccag ccatcagcgt cgctctcctg ctctcagtct tgcaggtgtc ccgagggcag     1620

aaggtgacca gcctgacagc ctgcctggtg aaccaaaacc ttcgcctgga ctgccgccat     1680

gagaataaca ccaaggataa ctccatccag catgagttca gcctgacccg agagaagagg     1740

aagcacgtgc tctcaggcac cctcgggata cccgagcaca cgtaccgctc ccgcgtcacc     1800

ctctccaacc agccctatat caaggtcctt accctagcca acttcaccac caaggatgag     1860

ggcgactact tttgtgagct tcgagtctcg ggcgcgaatc ccatgagctc caataaaagt     1920

atcagtgtgt atagagacaa actggtcaag tgtggcggca taagcctgct ggttcagaac     1980

acatcctgga tgctgctgct gctgctttcc ctctccctcc tccaagccct ggacttcatt     2040

tctctg                                                                2046


<210>  7
<211>  682
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR translation

<400>  7

Met Ala Ser Pro Leu Thr Arg Phe Leu Ser Leu Asn Leu Leu Leu Leu 
1               5                   10                  15      


Gly Glu Ser Ile Ile Leu Gly Ser Gly Glu Ala Ser Arg Gly Pro Leu 
            20                  25                  30          


Arg Pro Leu Cys Arg Pro Val Asn Ala Thr Leu Ala Ala Glu Asn Glu 
        35                  40                  45              


Phe Cys Pro Val Cys Ile Thr Phe Thr Thr Ser Ile Cys Ala Gly Tyr 
    50                  55                  60                  


Cys Pro Ser Met Val Arg Val Leu Pro Ala Ala Leu Pro Pro Val Pro 
65                  70                  75                  80  


Gln Pro Val Cys Thr Tyr Arg Glu Leu Arg Phe Ala Ser Val Arg Leu 
                85                  90                  95      


Pro Gly Cys Pro Pro Gly Val Asp Pro Ile Val Ser Phe Pro Val Ala 
            100                 105                 110         


Leu Ser Cys Arg Cys Gly Pro Cys Arg Leu Ser Ser Ser Asp Cys Gly 
        115                 120                 125             


Gly Pro Arg Thr Gln Pro Met Ala Cys Asp Leu Pro His Leu Pro Gly 
    130                 135                 140                 


Leu Leu Leu Leu Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
145                 150                 155                 160 


Gly Gly Ser Leu Pro Asp Gly Asp Phe Ile Ile Gln Gly Cys Pro Glu 
                165                 170                 175     


Cys Lys Leu Lys Glu Asn Lys Tyr Phe Ser Lys Leu Gly Ala Pro Ile 
            180                 185                 190         


Tyr Gln Cys Met Gly Cys Cys Phe Ser Arg Ala Tyr Pro Thr Pro Ala 
        195                 200                 205             


Arg Ser Lys Lys Thr Met Leu Val Pro Lys Asn Ile Thr Ser Glu Ala 
    210                 215                 220                 


Thr Cys Cys Val Ala Lys Ala Phe Thr Lys Ala Thr Val Met Gly Asn 
225                 230                 235                 240 


Ala Arg Val Glu Asn His Thr Glu Cys His Cys Ser Thr Cys Tyr Tyr 
                245                 250                 255     


His Lys Ser Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Ala Ala Ala 
            260                 265                 270         


Ser Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro Val His Pro 
        275                 280                 285             


Thr Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly 
    290                 295                 300                 


Ser Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp 
305                 310                 315                 320 


Ala Pro Leu Ala Gly Ile Cys Val Ala Leu Leu Leu Ser Leu Ile Ile 
                325                 330                 335     


Thr Leu Ile Cys Tyr Asn Ser Arg Arg Asn Arg Leu Leu Gln Ser Asp 
            340                 345                 350         


Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr 
        355                 360                 365             


Gln Pro Tyr Ala Pro Ala Arg Asp Phe Ala Ala Tyr Arg Pro Arg Ala 
    370                 375                 380                 


Lys Phe Ser Arg Ser Ala Glu Thr Ala Ala Asn Leu Gln Asp Pro Asn 
385                 390                 395                 400 


Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val 
                405                 410                 415     


Leu Glu Lys Lys Arg Ala Arg Asp Pro Glu Met Gly Gly Lys Gln Gln 
            420                 425                 430         


Arg Arg Arg Asn Pro Gln Glu Gly Val Tyr Asn Ala Leu Gln Lys Asp 
        435                 440                 445             


Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Thr Lys Gly Glu Arg Arg 
    450                 455                 460                 


Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr 
465                 470                 475                 480 


Lys Asp Thr Tyr Asp Ala Leu His Met Gln Thr Leu Ala Pro Arg Gly 
                485                 490                 495     


Thr Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val 
            500                 505                 510         


Glu Ser Asn Pro Gly Pro Gly Ser Met Asn Pro Ala Ile Ser Val Ala 
        515                 520                 525             


Leu Leu Leu Ser Val Leu Gln Val Ser Arg Gly Gln Lys Val Thr Ser 
    530                 535                 540                 


Leu Thr Ala Cys Leu Val Asn Gln Asn Leu Arg Leu Asp Cys Arg His 
545                 550                 555                 560 


Glu Asn Asn Thr Lys Asp Asn Ser Ile Gln His Glu Phe Ser Leu Thr 
                565                 570                 575     


Arg Glu Lys Arg Lys His Val Leu Ser Gly Thr Leu Gly Ile Pro Glu 
            580                 585                 590         


His Thr Tyr Arg Ser Arg Val Thr Leu Ser Asn Gln Pro Tyr Ile Lys 
        595                 600                 605             


Val Leu Thr Leu Ala Asn Phe Thr Thr Lys Asp Glu Gly Asp Tyr Phe 
    610                 615                 620                 


Cys Glu Leu Arg Val Ser Gly Ala Asn Pro Met Ser Ser Asn Lys Ser 
625                 630                 635                 640 


Ile Ser Val Tyr Arg Asp Lys Leu Val Lys Cys Gly Gly Ile Ser Leu 
                645                 650                 655     


Leu Val Gln Asn Thr Ser Trp Met Leu Leu Leu Leu Leu Ser Leu Ser 
            660                 665                 670         


Leu Leu Gln Ala Leu Asp Phe Ile Ser Leu 
        675                 680         


<210>  8
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR Signal Peptide

<400>  8

Met Ala Ser Pro Leu Thr Arg Phe Leu Ser Leu Asn Leu Leu Leu Leu 
1               5                   10                  15      


Gly Glu Ser Ile Ile Leu Gly Ser Gly Glu Ala 
            20                  25          


<210>  9
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH beta subunit of LH-CAR

<400>  9

Ser Arg Gly Pro Leu Arg Pro Leu Cys Arg Pro Val Asn Ala Thr Leu 
1               5                   10                  15      


Ala Ala Glu Asn Glu Phe Cys Pro Val Cys Ile Thr Phe Thr Thr Ser 
            20                  25                  30          


Ile Cys Ala Gly Tyr Cys Pro Ser Met Val Arg Val Leu Pro Ala Ala 
        35                  40                  45              


Leu Pro Pro Val Pro Gln Pro Val Cys Thr Tyr Arg Glu Leu Arg Phe 
    50                  55                  60                  


Ala Ser Val Arg Leu Pro Gly Cys Pro Pro Gly Val Asp Pro Ile Val 
65                  70                  75                  80  


Ser Phe Pro Val Ala Leu Ser Cys Arg Cys Gly Pro Cys Arg Leu Ser 
                85                  90                  95      


Ser Ser Asp Cys Gly Gly Pro Arg Thr Gln Pro Met Ala Cys Asp Leu 
            100                 105                 110         


Pro His Leu Pro Gly Leu Leu Leu Leu 
        115                 120     


<210>  10
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR linker subcomponent

<400>  10

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  11
<211>  96
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH alpha subunit of LH-CAR

<400>  11

Leu Pro Asp Gly Asp Phe Ile Ile Gln Gly Cys Pro Glu Cys Lys Leu 
1               5                   10                  15      


Lys Glu Asn Lys Tyr Phe Ser Lys Leu Gly Ala Pro Ile Tyr Gln Cys 
            20                  25                  30          


Met Gly Cys Cys Phe Ser Arg Ala Tyr Pro Thr Pro Ala Arg Ser Lys 
        35                  40                  45              


Lys Thr Met Leu Val Pro Lys Asn Ile Thr Ser Glu Ala Thr Cys Cys 
    50                  55                  60                  


Val Ala Lys Ala Phe Thr Lys Ala Thr Val Met Gly Asn Ala Arg Val 
65                  70                  75                  80  


Glu Asn His Thr Glu Cys His Cys Ser Thr Cys Tyr Tyr His Lys Ser 
                85                  90                  95      


<210>  12
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Myc tag of LH-CAR

<400>  12

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  13
<211>  44
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR CD8 Hinge

<400>  13

Ser Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro Val His Pro 
1               5                   10                  15      


Thr Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg Pro Arg Gly 
            20                  25                  30          


Ser Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp 
        35                  40                  


<210>  14
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR CD8 transmembrane domain

<400>  14

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Ile Cys Val Ala Leu Leu Leu 
1               5                   10                  15      


Ser Leu Ile Ile Thr Leu Ile Cys Tyr 
            20                  25  


<210>  15
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR CD28 cytoplasmic subcomponent

<400>  15

Asn Ser Arg Arg Asn Arg Leu Leu Gln Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      


Pro Arg Arg Pro Gly Leu Thr Arg Lys Pro Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          


Ala Arg Asp Phe Ala Ala Tyr Arg Pro 
        35                  40      


<210>  16
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR CD3 zeta subcomponent

<400>  16

Arg Ala Lys Phe Ser Arg Ser Ala Glu Thr Ala Ala Asn Leu Gln Asp 
1               5                   10                  15      


Pro Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Glu Lys Lys Arg Ala Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Gln Gln Arg Arg Arg Asn Pro Gln Glu Gly Val Tyr Asn Ala Leu Gln 
    50                  55                  60                  


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Thr Lys Gly Glu 
65                  70                  75                  80  


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Thr Leu Ala Pro 
            100                 105                 110         


Arg 
    


<210>  17
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR E2A subcomponent

<400>  17

Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp Val Glu Ser 
1               5                   10                  15      


Asn Pro Gly Pro 
            20  


<210>  18
<211>  162
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR Thy1.1 subcomponent

<400>  18

Met Asn Pro Ala Ile Ser Val Ala Leu Leu Leu Ser Val Leu Gln Val 
1               5                   10                  15      


Ser Arg Gly Gln Lys Val Thr Ser Leu Thr Ala Cys Leu Val Asn Gln 
            20                  25                  30          


Asn Leu Arg Leu Asp Cys Arg His Glu Asn Asn Thr Lys Asp Asn Ser 
        35                  40                  45              


Ile Gln His Glu Phe Ser Leu Thr Arg Glu Lys Arg Lys His Val Leu 
    50                  55                  60                  


Ser Gly Thr Leu Gly Ile Pro Glu His Thr Tyr Arg Ser Arg Val Thr 
65                  70                  75                  80  


Leu Ser Asn Gln Pro Tyr Ile Lys Val Leu Thr Leu Ala Asn Phe Thr 
                85                  90                  95      


Thr Lys Asp Glu Gly Asp Tyr Phe Cys Glu Leu Arg Val Ser Gly Ala 
            100                 105                 110         


Asn Pro Met Ser Ser Asn Lys Ser Ile Ser Val Tyr Arg Asp Lys Leu 
        115                 120                 125             


Val Lys Cys Gly Gly Ile Ser Leu Leu Val Gln Asn Thr Ser Trp Met 
    130                 135                 140                 


Leu Leu Leu Leu Leu Ser Leu Ser Leu Leu Gln Ala Leu Asp Phe Ile 
145                 150                 155                 160 


Ser Leu 
        


<210>  19
<211>  1443
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  LH-CAR cDNA

<400>  19
atggagacag acacactcct gctatgggtg ctgctgctct gggttccagg ttccacaggt       60

tccagggagc cgcttcggcc atggtgccac cccatcaatg ccatcctggc tgtcgagaag      120

gagggctgcc cagtgtgcat caccgtcaac accaccatct gtgccggcta ctgccccacc      180

atgatgcgcg tgctgcaggc ggtcctgccg cccctgcctc aggtggtgtg cacctaccgt      240

gatgtgcgct tcgagtccat ccggctccct ggctgcccgc gtggtgtgga ccccgtggtc      300

tccttccctg tggctctcag ctgtcgctgt ggaccctgcc gccgcagcac ctctgactgt      360

gggggtccca aagaccaccc cttgacctgt gaccaccccc aactctcagg cctcctcttc      420

ctcggtggag gtggatcagg tggaggtgga tctggtggag gtggatctgc tcctgatgtg      480

caggattgcc cagaatgcac gctacaggaa aacccattct tctcccagcc gggtgcccca      540

atacttcagt gcatgggctg ctgcttctct agagcatatc ccactccact aaggtccaag      600

aagacgatgt tggtccaaaa gaacgtcacc tcagagtcca cttgctgtgt agctaaatca      660

tataacaggg tcacagtaat ggggggtttc aaagtggaga accacacggc gtgccactgc      720

agtacttgtt attatcacaa atctgaacaa aaactcatct cagaagagga tctggcggcc      780

gcaattgaag ttatgtatcc tcctccttac ctagacaatg agaagagcaa tggaaccatt      840

atccatgtga aagggaaaca cctttgtcca agtcccctat ttcccggacc ttctaagccc      900

ttctgggtgc tggtggtggt gggcggggtg ctggcctgct acagcctgct ggtgacagtg      960

gccttcatca tcttttgggt gaaacggggc agaaagaaac tcctgtatat attcaaacaa     1020

ccatttatga gaccagtaca aactactcaa gaggaagatg gctgtagctg ccgatttcca     1080

gaagaagaag aaggaggatg tgaactgcgg gtgaagttca gcagaagcgc cgacgcccct     1140

gcctaccagc agggccagaa tcagctgtac aacgagctga acctgggcag aagggaagag     1200

tacgacgtcc tggataagcg gagaggccgg gaccctgaga tgggcggcaa gcctcggcgg     1260

aagaaccccc aggaaggcct gtataacgaa ctgcagaaag acaagatggc cgaggcctac     1320

agcgagatcg gcatgaaggg cgagcggagg cggggcaagg gccacgacgg cctgtatcag     1380

ggcctgtcca ccgccaccaa ggatacctac gacgccctgc acatgcaggc cctgccccca     1440

agg                                                                   1443


<210>  20
<211>  481
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR translation

<400>  20

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly Ser Arg Glu Pro Leu Arg Pro Trp Cys His Pro Ile 
            20                  25                  30          


Asn Ala Ile Leu Ala Val Glu Lys Glu Gly Cys Pro Val Cys Ile Thr 
        35                  40                  45              


Val Asn Thr Thr Ile Cys Ala Gly Tyr Cys Pro Thr Met Met Arg Val 
    50                  55                  60                  


Leu Gln Ala Val Leu Pro Pro Leu Pro Gln Val Val Cys Thr Tyr Arg 
65                  70                  75                  80  


Asp Val Arg Phe Glu Ser Ile Arg Leu Pro Gly Cys Pro Arg Gly Val 
                85                  90                  95      


Asp Pro Val Val Ser Phe Pro Val Ala Leu Ser Cys Arg Cys Gly Pro 
            100                 105                 110         


Cys Arg Arg Ser Thr Ser Asp Cys Gly Gly Pro Lys Asp His Pro Leu 
        115                 120                 125             


Thr Cys Asp His Pro Gln Leu Ser Gly Leu Leu Phe Leu Gly Gly Gly 
    130                 135                 140                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Pro Asp Val 
145                 150                 155                 160 


Gln Asp Cys Pro Glu Cys Thr Leu Gln Glu Asn Pro Phe Phe Ser Gln 
                165                 170                 175     


Pro Gly Ala Pro Ile Leu Gln Cys Met Gly Cys Cys Phe Ser Arg Ala 
            180                 185                 190         


Tyr Pro Thr Pro Leu Arg Ser Lys Lys Thr Met Leu Val Gln Lys Asn 
        195                 200                 205             


Val Thr Ser Glu Ser Thr Cys Cys Val Ala Lys Ser Tyr Asn Arg Val 
    210                 215                 220                 


Thr Val Met Gly Gly Phe Lys Val Glu Asn His Thr Ala Cys His Cys 
225                 230                 235                 240 


Ser Thr Cys Tyr Tyr His Lys Ser Glu Gln Lys Leu Ile Ser Glu Glu 
                245                 250                 255     


Asp Leu Ala Ala Ala Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp 
            260                 265                 270         


Asn Glu Lys Ser Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu 
        275                 280                 285             


Cys Pro Ser Pro Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu 
    290                 295                 300                 


Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val 
305                 310                 315                 320 


Ala Phe Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
                325                 330                 335     


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
            340                 345                 350         


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
        355                 360                 365             


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln 
    370                 375                 380                 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
385                 390                 395                 400 


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
                405                 410                 415     


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
            420                 425                 430         


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
        435                 440                 445             


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
    450                 455                 460                 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
465                 470                 475                 480 


Arg 
    


<210>  21
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR signal peptide

<400>  21

Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val Pro 
1               5                   10                  15      


Gly Ser Thr Gly 
            20  


<210>  22
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH beta subunit of LH-CAR

<400>  22

Ser Arg Glu Pro Leu Arg Pro Trp Cys His Pro Ile Asn Ala Ile Leu 
1               5                   10                  15      


Ala Val Glu Lys Glu Gly Cys Pro Val Cys Ile Thr Val Asn Thr Thr 
            20                  25                  30          


Ile Cys Ala Gly Tyr Cys Pro Thr Met Met Arg Val Leu Gln Ala Val 
        35                  40                  45              


Leu Pro Pro Leu Pro Gln Val Val Cys Thr Tyr Arg Asp Val Arg Phe 
    50                  55                  60                  


Glu Ser Ile Arg Leu Pro Gly Cys Pro Arg Gly Val Asp Pro Val Val 
65                  70                  75                  80  


Ser Phe Pro Val Ala Leu Ser Cys Arg Cys Gly Pro Cys Arg Arg Ser 
                85                  90                  95      


Thr Ser Asp Cys Gly Gly Pro Lys Asp His Pro Leu Thr Cys Asp His 
            100                 105                 110         


Pro Gln Leu Ser Gly Leu Leu Phe Leu 
        115                 120     


<210>  23
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH alpha subunit of LH-CAR

<400>  23

Ala Pro Asp Val Gln Asp Cys Pro Glu Cys Thr Leu Gln Glu Asn Pro 
1               5                   10                  15      


Phe Phe Ser Gln Pro Gly Ala Pro Ile Leu Gln Cys Met Gly Cys Cys 
            20                  25                  30          


Phe Ser Arg Ala Tyr Pro Thr Pro Leu Arg Ser Lys Lys Thr Met Leu 
        35                  40                  45              


Val Gln Lys Asn Val Thr Ser Glu Ser Thr Cys Cys Val Ala Lys Ser 
    50                  55                  60                  


Tyr Asn Arg Val Thr Val Met Gly Gly Phe Lys Val Glu Asn His Thr 
65                  70                  75                  80  


Ala Cys His Cys Ser Thr Cys Tyr Tyr His Lys Ser 
                85                  90          


<210>  24
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR CD28 hinge

<400>  24

Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser Asn 
1               5                   10                  15      


Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro Leu 
            20                  25                  30          


Phe Pro Gly Pro Ser Lys Pro 
        35                  


<210>  25
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR CD28 transmembrane domain

<400>  25

Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      


Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          


<210>  26
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR 4-1BB subcomponent

<400>  26

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  27
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  LH-CAR CD3 zeta subcomponent

<400>  27

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  28
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRH1 of anti-LHR Binding Agent

<400>  28

Gly Tyr Ser Ile Thr Ser Gly Tyr Gly 
1               5                   


<210>  29
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRH2 of anti-LHR Binding Agent

<400>  29

Ile His Tyr Ser Gly Ser Thr 
1               5           


<210>  30
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRH3 of anti-LHR Binding Agent

<400>  30

Ala Arg Ser Leu Arg Tyr 
1               5       


<210>  31
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRL1 of anti-LHR Binding Agent

<400>  31

Ser Ser Val Asn Tyr 
1               5   


<210>  32
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRL3 of anti-LHR Binding Agent

<400>  32

His Gln Trp Ser Ser Tyr Pro Tyr Thr 
1               5                   


<210>  33
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRH1 of anti-LHR Binding Agent

<400>  33

Gly Phe Ser Leu Thr Thr Tyr Gly 
1               5               


<210>  34
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRH2 of anti-LHR Binding Agent

<400>  34

Ile Trp Gly Asp Gly Ser Thr 
1               5           


<210>  35
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRH3 of anti-LHR Binding Agent

<400>  35

Ala Glu Gly Ser Ser Leu Phe Ala Tyr 
1               5                   


<210>  36
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRL1 of anti-LHR Binding Agent

<400>  36

Gln Ser Leu Leu Asn Ser Gly Asn Gln Lys Asn Tyr 
1               5                   10          


<210>  37
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRL3 of anti-LHR Binding Agent

<400>  37

Gln Asn Asp Tyr Ser Tyr Pro Leu Thr 
1               5                   


<210>  38
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRH1 of anti-LHR Binding Agent

<400>  38

Gly Tyr Ser Phe Thr Gly Tyr Tyr 
1               5               


<210>  39
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRH2 of anti-LHR Binding Agent

<400>  39

Ile Tyr Pro Tyr Asn Gly Val Ser 
1               5               


<210>  40
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRH3 of anti-LHR Binding Agent

<400>  40

Ala Arg Glu Arg Gly Leu Tyr Gln Leu Arg Ala Met Asp Tyr 
1               5                   10                  


<210>  41
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRL1 of anti-LHR Binding Agent

<400>  41

Gln Ser Ile Ser Asn Asn 
1               5       


<210>  42
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDRL3 of anti-LHR Binding Agent

<400>  42

Gln Gln Ser Asn Ser Trp Pro Tyr Thr 
1               5                   


<210>  43
<211>  111
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-LHR Binding Agent includes a heavy chain

<400>  43

Glu Val Gln Leu Gln Glu Ser Gly Pro Asp Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Leu Thr Cys Thr Val Thr Gly Tyr Ser Ile Thr Ser Gly 
            20                  25                  30          


Tyr Gly Trp His Arg Gln Phe Pro Gly Asn Lys Leu Glu Trp Met Gly 
        35                  40                  45              


Tyr Ile His Tyr Ser Gly Ser Thr Thr Tyr Asn Pro Ser Leu Lys Ser 
    50                  55                  60                  


Arg Ile Ser Ile Ser Arg Asp Thr Ser Lys Asn Gln Phe Phe Leu Gln 
65                  70                  75                  80  


Leu Asn Ser Val Thr Thr Glu Asp Thr Ala Thr Tyr Tyr Cys Ala Arg 
                85                  90                  95      


Ser Leu Arg Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser 
            100                 105                 110     


<210>  44
<211>  106
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-LHR Binding Agent includes a light chain

<400>  44

Asp Ile Val Met Thr Gln Thr Pro Ala Ile Met Ser Ala Ser Pro Gly 
1               5                   10                  15      


Gln Lys Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Asn Tyr Met 
            20                  25                  30          


His Trp Tyr Gln Gln Lys Leu Gly Ser Ser Pro Lys Leu Trp Ile Tyr 
        35                  40                  45              


Asp Thr Ser Lys Leu Ala Pro Gly Val Pro Ala Arg Phe Ser Gly Ser 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Ser Met Glu Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Ser Tyr Phe Cys His Gln Trp Ser Ser Tyr Pro Tyr Thr 
                85                  90                  95      


Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105     


<210>  45
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-LHR Binding Agent includes a heavy chain

<400>  45

Gln Val Gln Leu Lys Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln 
1               5                   10                  15      


Ser Leu Ser Arg Arg Cys Thr Val Ser Gly Phe Ser Leu Thr Thr Tyr 
            20                  25                  30          


Gly Val Ser Trp Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Leu 
        35                  40                  45              


Gly Val Ile Trp Gly Asp Gly Ser Thr Tyr Tyr His Ser Ala Leu Ile 
    50                  55                  60                  


Ser Arg Leu Ser Ile Ser Lys Asp Asn Ser Lys Ser Gln Val Phe Leu 
65                  70                  75                  80  


Lys Leu Asn Ser Leu Gln Thr Asp Asp Thr Ala Thr Tyr Tyr Cys Ala 
                85                  90                  95      


Glu Gly Ser Ser Leu Phe Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr 
            100                 105                 110         


Val Ser Ala 
        115 


<210>  46
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-LHR Binding Agent includes a light chain


<220>
<221>  misc_feature
<222>  (89)..(89)
<223>  Xaa can be any naturally occurring amino acid

<400>  46

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Thr Val Thr Ala Gly 
1               5                   10                  15      


Glu Lys Val Thr Met Ser Cys Lys Ser Ser Gln Ser Leu Leu Asn Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Thr Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Pro Pro Lys Leu Leu Ile Tyr Trp Ala Ser Thr Arg Gln Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Ser Val Gln Ala Glu Asp Xaa Ala Val Tyr Tyr Cys Gln Asn 
                85                  90                  95      


Asp Tyr Ser Tyr Pro Leu Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile 
            100                 105                 110         


Lys 
    


<210>  47
<211>  103
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-LHR Binding Agent includes a heavy chain

<400>  47

Glu Val Gln Leu Glu Gln Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Arg Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Phe 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Glu Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Tyr Ile Ser Ser Gly Ser Ser Thr Leu His Tyr Ala Asp Thr Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Pro Lys Asn Thr Leu Phe 
65                  70                  75                  80  


Leu Gln Met Lys Leu Pro Ser Leu Cys Tyr Gly Leu Leu Gly Ser Arg 
                85                  90                  95      


Asn Leu Ser His Arg Leu Leu 
            100             


<210>  48
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-LHR Binding Agent includes a light chain

<400>  48

Asp Ile Val Leu Thr Gln Thr Pro Ser Ser Leu Ser Ala Ser Leu Gly 
1               5                   10                  15      


Asp Thr Ile Thr Ile Thr Cys His Ala Ser Gln Asn Ile Asn Val Trp 
            20                  25                  30          


Leu Phe Trp Tyr Gln Gln Lys Pro Gly Asn Ile Pro Lys Leu Leu Ile 
        35                  40                  45              


Tyr Lys Ala Ser Asn Leu Leu Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Gly Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Ile Ala Thr Tyr Tyr Cys Gln Gln Gly Gln Ser Phe Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  49
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-LHR Binding Agent includes a heavy chain

<400>  49

Gln Val Lys Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            20                  25                  30          


Tyr Met His Trp Val Lys Gln Ser His Gly Asn Ile Leu Asp Trp Ile 
        35                  40                  45              


Gly Tyr Ile Tyr Pro Tyr Asn Gly Val Ser Ser Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Arg Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Glu Arg Gly Leu Tyr Gln Leu Arg Ala Met Asp Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Ser Val Thr Val Ser Ser 
        115                 120     


<210>  50
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Anti-LHR Binding Agent includes a light chain

<400>  50

Asp Ile Val Leu Thr Gln Thr Pro Ala Thr Leu Ser Val Thr Pro Gly 
1               5                   10                  15      


Asp Ser Val Ser Leu Ser Cys Arg Ala Ser Gln Ser Ile Ser Asn Asn 
            20                  25                  30          


Leu His Trp Tyr Gln Gln Lys Ser His Glu Ser Pro Arg Leu Leu Ile 
        35                  40                  45              


Lys Asn Ala Ser Gln Ser Ile Ser Gly Ile Pro Ser Lys Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Arg Ile Asn Ser Val Glu Thr 
65                  70                  75                  80  


Glu Asp Phe Gly Met Tyr Phe Cys Gln Gln Ser Asn Ser Trp Pro Tyr 
                85                  90                  95      


Thr Phe Gly Ser Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  51
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Recombinant LH agonist

<400>  51

Glu His Trp Ser Tyr Gly Leu Arg Pro Gly 
1               5                   10  


<210>  52
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant Fc region

<400>  52

Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys Pro 
1               5                   10                  15  


<210>  53
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant Fc region

<400>  53

Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro 
1               5                   10          


<210>  54
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant Fc region

<400>  54

Glu Leu Lys Thr Pro Leu Gly Asp Thr His Thr Cys Pro Arg Cys Pro 
1               5                   10                  15      


<210>  55
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant Fc region

<400>  55

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro 
1               5                   10          


<210>  56
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant Fc region

<400>  56

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10          


<210>  57
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant Fc region

<400>  57

Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   


<210>  58
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant Fc region

<400>  58

Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10  


<210>  59
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Variant Fc region

<400>  59

Glu Val Val Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro 
1               5                   10              


<210>  60
<211>  220
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD28 signaling region

<400>  60

Met Leu Arg Leu Leu Leu Ala Leu Asn Leu Phe Pro Ser Ile Gln Val 
1               5                   10                  15      


Thr Gly Asn Lys Ile Leu Val Lys Gln Ser Pro Met Leu Val Ala Tyr 
            20                  25                  30          


Asp Asn Ala Val Asn Leu Ser Cys Lys Tyr Ser Tyr Asn Leu Phe Ser 
        35                  40                  45              


Arg Glu Phe Arg Ala Ser Leu His Lys Gly Leu Asp Ser Ala Val Glu 
    50                  55                  60                  


Val Cys Val Val Tyr Gly Asn Tyr Ser Gln Gln Leu Gln Val Tyr Ser 
65                  70                  75                  80  


Lys Thr Gly Phe Asn Cys Asp Gly Lys Leu Gly Asn Glu Ser Val Thr 
                85                  90                  95      


Phe Tyr Leu Gln Asn Leu Tyr Val Asn Gln Thr Asp Ile Tyr Phe Cys 
            100                 105                 110         


Lys Ile Glu Val Met Tyr Pro Pro Pro Tyr Leu Asp Asn Glu Lys Ser 
        115                 120                 125             


Asn Gly Thr Ile Ile His Val Lys Gly Lys His Leu Cys Pro Ser Pro 
    130                 135                 140                 


Leu Phe Pro Gly Pro Ser Lys Pro Phe Trp Val Leu Val Val Val Gly 
145                 150                 155                 160 


Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile 
                165                 170                 175     


Phe Trp Val Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met 
            180                 185                 190         


Asn Met Thr Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro 
        195                 200                 205             


Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
    210                 215                 220 


<210>  61
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM1

<400>  61

Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn 
1               5                   10                  15      


Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg 
            20                  25                  


<210>  62
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM2

<400>  62

Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
1               5                   10                  15      


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met 
            20                  25                  


<210>  63
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ITAM3

<400>  63

Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
1               5                   10                  15      


Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln 
            20                  25                  


<210>  64
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG1 heavy chain hinge sequence

<400>  64

Cys Thr Cys Gly Ala Gly Cys Cys Cys Ala Ala Ala Thr Cys Thr Thr 
1               5                   10                  15      


Gly Thr Gly Ala Cys Ala Ala Ala Ala Cys Thr Cys Ala Cys Ala Cys 
            20                  25                  30          


Ala Thr Gly Cys Cys Cys Ala Cys Cys Gly Thr Gly Cys Cys Cys Gly 
        35                  40                  45              


<210>  65
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge domain

<400>  65

Pro Ala Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala 
1               5                   10                  15      


Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg 
            20                  25                  30          


Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys 
        35                  40                  45              


Asp Ile Tyr 
    50      


<210>  66
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge domain

<400>  66

Lys Val Asn Ser Thr Thr Thr Lys Pro Val Leu Arg Thr Pro Ser Pro 
1               5                   10                  15      


Val His Pro Thr Gly Thr Ser Gln Pro Gln Arg Pro Glu Asp Cys Arg 
            20                  25                  30          


Pro Arg Gly Ser Val Lys Gly Thr Gly Leu Asp Phe Ala Cys Asp Ile 
        35                  40                  45              


Tyr 
    


<210>  67
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 hinge domain

<400>  67

Pro Val Lys Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Gln Ala 
1               5                   10                  15      


Pro Ile Thr Thr Ser Gln Arg Val Ser Leu Arg Pro Gly Thr Cys Gln 
            20                  25                  30          


Pro Ser Ala Gly Ser Thr Val Glu Ala Ser Gly Leu Asp Leu Ser Cys 
        35                  40                  45              


Asp Ile Tyr 
    50      


<210>  68
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  intermediate length spacer including an IgG4 hinge and a CH3 
       region

<400>  68

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Gly Gln Pro Arg 
1               5                   10                  15      


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
            20                  25                  30          


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
        35                  40                  45              


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
    50                  55                  60                  


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
65                  70                  75                  80  


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
                85                  90                  95      


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
            100                 105                 110         


Leu Ser Leu Ser Leu Gly Lys 
        115                 


<210>  69
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  longer length spacer including an IgG4 hinge and a CH3 region

<400>  69

Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu Phe 
1               5                   10                  15      


Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ser 
            100                 105                 110         


Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Leu Gly Lys 
225                 


<210>  70
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane domain

<400>  70

Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu 
1               5                   10                  15      


Ser Leu Val Ile Thr 
            20      


<210>  71
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD8 transmembrane domain

<400>  71

Ile Trp Ala Pro Leu Ala Gly Ile Cys Ala Val Leu Leu Leu Ser Leu 
1               5                   10                  15      


Val Ile Thr Leu Ile 
            20      


<210>  72
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR linker sequence

<400>  72

Gly Gly Gly Ser Gly Gly Gly Ser 
1               5               


<210>  73
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR linker sequence

<400>  73

Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser 
1               5                   10      


<210>  74
<211>  110
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CH2 sequence

<400>  74

Ala Pro Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
1               5                   10                  15      


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            20                  25                  30          


Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr 
        35                  40                  45              


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    50                  55                  60                  


Gln Phe Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His 
65                  70                  75                  80  


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                85                  90                  95      


Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys 
            100                 105                 110 


<210>  75
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CH3 sequence

<400>  75

Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu 
1               5                   10                  15      


Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe 
            20                  25                  30          


Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu 
        35                  40                  45              


Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe 
    50                  55                  60                  


Phe Leu Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly 
65                  70                  75                  80  


Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr 
                85                  90                  95      


Thr Gln Lys Ser Leu Ser Leu Ser Leu Gly Lys 
            100                 105         


<210>  76
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STREP tag

<400>  76

Trp Arg His Pro Gln Phe Gly Gly 
1               5               


<210>  77
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  STREP TAG II

<400>  77

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  78
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flag Tag

<400>  78

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  79
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Xpress tag

<400>  79

Asp Leu Tyr Asp Asp Asp Asp Lys 
1               5               


<210>  80
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Avi tag

<400>  80

Gly Leu Asn Asp Ile Phe Glu Ala Gln Lys Ile Glu Trp His Glu 
1               5                   10                  15  


<210>  81
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Calmodulin tag

<400>  81

Lys Arg Arg Trp Lys Lys Asn Phe Ile Ala Val Ser Ala Ala Asn Arg 
1               5                   10                  15      


Phe Lys Lys Ile Ser Ser Ser Gly Ala Leu 
            20                  25      


<210>  82
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HA tag

<400>  82

Tyr Pro Tyr Asp Val Pro Asp Tyr Ala 
1               5                   


<210>  83
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Softag1

<400>  83

Ser Leu Ala Glu Leu Leu Asn Ala Gly Leu Gly Gly Ser 
1               5                   10              


<210>  84
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Softag3

<400>  84

Thr Gln Asp Pro Ser Arg Val Gly 
1               5               


<210>  85
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  V5 tag

<400>  85

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  86
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A

<400>  86

Leu Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp 
1               5                   10                  15      


Val Glu Glu Asn Pro Gly Pro Arg 
            20                  


<210>  87
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  T2A

<400>  87

Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210>  88
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P2A

<400>  88

Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210>  89
<211>  24
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  E2A

<400>  89

Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro Pro 
            20                  


<210>  90
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  F2A

<400>  90

Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


