                               SEQUENCE LISTING

<110> ASTRAZENECA AB
 
<120> CELL LINES AND METHODS FOR INCREASED PROTEIN PRODUCTION

<130> 0098-0001WO1

<140>
<141>

<150> 62/566,681
<151> 2017-10-02

<160> 6     

<170> PatentIn version 3.5

<210> 1
<211> 5239
<212> DNA
<213> Homo sapiens

<400> 1
ggatccggat tcggattagc agcccgggaa gagtgccgtg gcacaggcgc cggagggagc       60

gcgaccctcg gaccccgcct ggcccgcggg gctgggaccc ggccccggcc tgcccgatgg      120

ggcgcgcggc cccggagatg cgccctcgcc cggccccgcg cccccggccc cgcgcccccg      180

gcccgcccgc cccggcccgc gcctccgcct gagtcccccg cgccttggcc cgccaccccc      240

cgccccgcgc ccccggcccg cctgcgccat ggagcccggc cgcggcggca cagagaccgt      300

gggcaagttc gagttctccc gcaaggacct gatcggccac ggcgccttcg cggtggtctt      360

caagggccgc caccgcgaga agcacgattt ggaggtcgcc gtcaagtgca ttaacaagaa      420

gaacctcgcc aagtctcaga cgctgctggg gaaggaaatc aaaatcctga aggaactgaa      480

acatgaaaac atcgtggccc tgtacgactt ccaggaaatg gctaattctg tctacctggt      540

tatggagtac tgcaacggtg gggacctggc cgactacctg cacgccatgc gcacgctgag      600

cgaggacacc atcaggctct tcctgcagca gatcgcgggc gccatgcggc ttctgcacag      660

caaaggcatc atccaccgcg acctgaaacc gcagaacatc ctgctgtcca accccgccgg      720

ccgccgcgcc aaccccaaca gcatccgcgt caagatcgct gacttcggct tcgcgcggta      780

cctccagagc aacatgatgg cggccacact ctgcggctcc cccatgtaca tggcccccga      840

ggtcatcatg tcccagcact acgacgggaa ggcggacctg tggagcatcg gcaccatcgt      900

ctaccagtgc ctgacgggga aggcgccctt ccaggccagc agcccccagg acctgcgcct      960

gttctacgag aagaacaaga cgttggtccc caccatcccc cgggagacct cggccccgct     1020

gcggcagctg ctcctggccc tactgcaacg caaccacaag gaccgcatgg acttcgatga     1080

gttttttcat caccctttcc tcgatgccag cccctcggtc aggaaatccc cacccgtgcc     1140

tgtgccctcg tacccaagct cggggtccgg cagcagctcc agcagcagct ccacctccca     1200

cctggcctcc ccgccgtccc tgggcgagat gcagcagctg cagaagaccc tggcctcccc     1260

ggctgacacc gctggcttcc tgcacagctc ccgggactct ggtggcagca aggactcttc     1320

ctgtgacaca gacgacttcg tcatggtccc cgcgcagttt ccaggtgacc tggtggctga     1380

ggcgcccagt gccaaacccc cgccagacag cctgatgtgc agtgggagct cactggtggc     1440

ctctgcgggc ttggagagcc acggccggac cccatctcca tccccaccct gcagcagctc     1500

ccccagtccc tcaggccggg ctggcccgtt ctccagcagc aggtgcggcg cctctgtccc     1560

catcccagtc cccacgcagg tgcagaacta ccagcgcatt gagcgaaacc tgcagtcacc     1620

cacccagttc caaacacctc ggtcctctgc catccgcagg tcaggcagca ccagccccct     1680

gggctttgca agggccagcc cctcgccccc tgcccacgct gagcatggag gcgtcctggc     1740

caggaagatg tctctgggtg gaggccggcc ctacacgcca tctcctcaag ttggaaccat     1800

ccctgagcgg ccaggctgga gcgggacgcc ctccccacag ggagctgaga tgcggggtgg     1860

caggtcccct cgtccaggct cctctgcacc cgagcactct ccccgcactt ccgggctggg     1920

ctgccgcctg cacagcgccc ccaacctgtc tgacttgcac gtcgtccgcc ccaagctgcc     1980

caaacccccc acggaccccc tgggagctgt gttcagccca ccacaggcca gccctcccca     2040

gccgtcccac ggcctgcagt cctgccggaa cctgcggggc tcacccaagc tgcccgactt     2100

cctgcagcga aaccccctgc cccccatcct gggctccccc accaaggctg tgccctcctt     2160

tgacttcccg aagaccccca gctcccagaa cctgctggcc ctcctagccc ggcagggcgt     2220

ggtgatgacg ccccctcgaa accggacgct gcccgacctc tcggaggtgg gacccttcca     2280

tggtcagccg ttgggccctg gcctgcggcc aggcgaggac cccaagggcc cctttggccg     2340

gtctttcagc accagccgcc tcactgacct gctccttaag gcggcgtttg ggacacaagc     2400

cccggacccg ggcagcacgg agagcctgca ggagaagccc atggagatcg caccctcagc     2460

tggctttgga gggagcctgc acccaggagc ccgtgctggg ggcaccagca gcccttcccc     2520

ggtggtcttc accgtgggct ctcccccgag cgggagcacg cccccccagg gcccccgcac     2580

caggatgttc tcagcgggcc ccactggctc tgccagctct tctgcccgcc acctggtgcc     2640

tgggccctgc agcgaggccc cagcccctga gctccctgct ccaggacacg gctgcagctt     2700

tgccgacccc attgctgcga acctggaggg ggctgtgacc ttcgaggccc ccgacctccc     2760

tgaggagacc ctcatggagc aagagcacac ggagatcctg cgtggcctgc gcttcacgct     2820

gctgttcgtg cagcacgtcc tggagatcgc agccctgaag ggcagcgcca gtgaggcggc     2880

ggggggccct gagtaccagc tgcaggagag tgtggtggcc gaccagatca gcctgctgag     2940

ccgagaatgg ggcttcgcgg aacagctggt gctgtacctg aaggtggccg agctactgtc     3000

ctccggcctg caaagtgcca tcgaccagat ccgggccggc aagctctgcc tgtcgtccac     3060

tgtgaagcag gtggtgcgca ggctgaatga gctgtacaag gccagcgtgg tgtcctgcca     3120

gggcctgagc ctgcggctgc agcgcttctt cctggacaag cagcggctcc tggaccgcat     3180

tcacagcatc actgccgaga ggctcatctt cagccacgct gtgcagatgg tgcagtcggc     3240

tgccctggac gagatgttcc agcaccgtga gggctgcgtc ccacgctacc acaaggccct     3300

gctgctcctg gaggggctgc agcacatgct ctcggaccag gccgacatcg agaacgtcac     3360

caagtgcaag ctgtgcattg agcggagact ctcggcgctg ctgactggca tctgtgcctg     3420

acctttctgg cctggctggg ccccccgtcc tgccgagccc tgcagagtgg gctctgtgtg     3480

ctggctggac tcctcgggac aagcccatgg cgctgatcgc tggtgctgag ccctgccctg     3540

ggccccacgg acagtcagcc tgccggcctc cctgcagctc acggggcaga accagcacat     3600

ctggagccac acagcttggg gggtgtctcc catcttttac aggtggggat cacagaattt     3660

ctgcccctcc agctgcctgg ctcagcaggc gtgggtgcca ccaccctcta gccccagggc     3720

agccccggag gacaggcaag ggcctgagac cactgccgac tcaaagccaa agcgagctcc     3780

tgcttagggc aggtcagcag gcactgtgcc caggaagagc ctgcggcctc ggcgtccccc     3840

agtctccagg agcctctccc tccgagatac ccacccagct ttgtcaatca cccaagcact     3900

ttatgcatat agagacagaa cctggacctc accagggact gctgggcagc gattcctggc     3960

agtggcctgg tgtttgtaca tacacatatg cagacacatg ccagggcccc ccaagcccga     4020

gcaccggacc acgttgctgc ccaggtctgg acctcagcgg gagaactggc tccgggggga     4080

gtggggccct gcgctagagg cagaggcagt tctttgttca agcgttcctc tggggaccgg     4140

cagcagaggc accgtgttct ctcagccctg gatacgtctt gtaatctttc acactttatt     4200

cctaaaacgt gtcttatttt tatgcagctc attttttctt taaaggagaa aacttgtagg     4260

tgtttaagaa ttggttttgg gagggcgagg actgggccag gttagaggca gatggcacag     4320

gggcgtgtgg cgggcgggtg aggctgcttt gcacacctgt gttggtggct gtcccctgcc     4380

gcccctccct gtggcagcag caggacaggt gtgtgcccag caccctccct acctgggcct     4440

ggaagcagat gaggggaata cttcatgcaa agaaaaaagt aacatgtgca aaagctcccc     4500

gtccagcttt gacagtcagt tttgatgtca gctcctcggc agggtaggcc tgatgacagc     4560

cctgtccctc cctgcctctg ccttgcccaa ggccacggag ggcatctgca gagaggcctg     4620

ccttccggat tccaggcggg catgccctgc aaaccccgcc tgggcctccc ttggtctgcc     4680

cagccctcgg ttagccctgc ctgaatcagt agatacttga acgagtcccc agtctgcggg     4740

aggcagtggt ggggccatgg acccatgcgg ggggttccag ggtcacacgc cacataacag     4800

acaaaaatac acacacgtgt gtttttcttt gcaatacttg aaatattgcc actgtgcttg     4860

gacttagaag aagaaaatcc ccgtgacttc ttcctcatca ccttgatggc tttattctca     4920

ccttgtgggg catgtttgta tttattgctt catggccgac tggaatcctg agtcctggga     4980

agctggcact gcggggatct tgcccggtgt cctggtcctc ttgcttccgt cgcggccgca     5040

tgtgcgtgtg tccaagcagg tcctgggcgc ctcaactgct gcccctggtt gaatgttctc     5100

ttgatagtgc tggacccttt gtctatttta aagcgaattt tgtgtgattt cctgcccttt     5160

gcgttatatt gtataatacc aacgtaagga aataaacctt tggaattgtt gggctggtgt     5220

caaaaaaaaa aaaaaaaaa                                                  5239


<210> 2
<211> 1050
<212> PRT
<213> Homo sapiens

<400> 2
Met Glu Pro Gly Arg Gly Gly Thr Glu Thr Val Gly Lys Phe Glu Phe 
1               5                   10                  15      


Ser Arg Lys Asp Leu Ile Gly His Gly Ala Phe Ala Val Val Phe Lys 
            20                  25                  30          


Gly Arg His Arg Glu Lys His Asp Leu Glu Val Ala Val Lys Cys Ile 
        35                  40                  45              


Asn Lys Lys Asn Leu Ala Lys Ser Gln Thr Leu Leu Gly Lys Glu Ile 
    50                  55                  60                  


Lys Ile Leu Lys Glu Leu Lys His Glu Asn Ile Val Ala Leu Tyr Asp 
65                  70                  75                  80  


Phe Gln Glu Met Ala Asn Ser Val Tyr Leu Val Met Glu Tyr Cys Asn 
                85                  90                  95      


Gly Gly Asp Leu Ala Asp Tyr Leu His Ala Met Arg Thr Leu Ser Glu 
            100                 105                 110         


Asp Thr Ile Arg Leu Phe Leu Gln Gln Ile Ala Gly Ala Met Arg Leu 
        115                 120                 125             


Leu His Ser Lys Gly Ile Ile His Arg Asp Leu Lys Pro Gln Asn Ile 
    130                 135                 140                 


Leu Leu Ser Asn Pro Ala Gly Arg Arg Ala Asn Pro Asn Ser Ile Arg 
145                 150                 155                 160 


Val Lys Ile Ala Asp Phe Gly Phe Ala Arg Tyr Leu Gln Ser Asn Met 
                165                 170                 175     


Met Ala Ala Thr Leu Cys Gly Ser Pro Met Tyr Met Ala Pro Glu Val 
            180                 185                 190         


Ile Met Ser Gln His Tyr Asp Gly Lys Ala Asp Leu Trp Ser Ile Gly 
        195                 200                 205             


Thr Ile Val Tyr Gln Cys Leu Thr Gly Lys Ala Pro Phe Gln Ala Ser 
    210                 215                 220                 


Ser Pro Gln Asp Leu Arg Leu Phe Tyr Glu Lys Asn Lys Thr Leu Val 
225                 230                 235                 240 


Pro Thr Ile Pro Arg Glu Thr Ser Ala Pro Leu Arg Gln Leu Leu Leu 
                245                 250                 255     


Ala Leu Leu Gln Arg Asn His Lys Asp Arg Met Asp Phe Asp Glu Phe 
            260                 265                 270         


Phe His His Pro Phe Leu Asp Ala Ser Pro Ser Val Arg Lys Ser Pro 
        275                 280                 285             


Pro Val Pro Val Pro Ser Tyr Pro Ser Ser Gly Ser Gly Ser Ser Ser 
    290                 295                 300                 


Ser Ser Ser Ser Thr Ser His Leu Ala Ser Pro Pro Ser Leu Gly Glu 
305                 310                 315                 320 


Met Gln Gln Leu Gln Lys Thr Leu Ala Ser Pro Ala Asp Thr Ala Gly 
                325                 330                 335     


Phe Leu His Ser Ser Arg Asp Ser Gly Gly Ser Lys Asp Ser Ser Cys 
            340                 345                 350         


Asp Thr Asp Asp Phe Val Met Val Pro Ala Gln Phe Pro Gly Asp Leu 
        355                 360                 365             


Val Ala Glu Ala Pro Ser Ala Lys Pro Pro Pro Asp Ser Leu Met Cys 
    370                 375                 380                 


Ser Gly Ser Ser Leu Val Ala Ser Ala Gly Leu Glu Ser His Gly Arg 
385                 390                 395                 400 


Thr Pro Ser Pro Ser Pro Pro Cys Ser Ser Ser Pro Ser Pro Ser Gly 
                405                 410                 415     


Arg Ala Gly Pro Phe Ser Ser Ser Arg Cys Gly Ala Ser Val Pro Ile 
            420                 425                 430         


Pro Val Pro Thr Gln Val Gln Asn Tyr Gln Arg Ile Glu Arg Asn Leu 
        435                 440                 445             


Gln Ser Pro Thr Gln Phe Gln Thr Pro Arg Ser Ser Ala Ile Arg Arg 
    450                 455                 460                 


Ser Gly Ser Thr Ser Pro Leu Gly Phe Ala Arg Ala Ser Pro Ser Pro 
465                 470                 475                 480 


Pro Ala His Ala Glu His Gly Gly Val Leu Ala Arg Lys Met Ser Leu 
                485                 490                 495     


Gly Gly Gly Arg Pro Tyr Thr Pro Ser Pro Gln Val Gly Thr Ile Pro 
            500                 505                 510         


Glu Arg Pro Gly Trp Ser Gly Thr Pro Ser Pro Gln Gly Ala Glu Met 
        515                 520                 525             


Arg Gly Gly Arg Ser Pro Arg Pro Gly Ser Ser Ala Pro Glu His Ser 
    530                 535                 540                 


Pro Arg Thr Ser Gly Leu Gly Cys Arg Leu His Ser Ala Pro Asn Leu 
545                 550                 555                 560 


Ser Asp Leu His Val Val Arg Pro Lys Leu Pro Lys Pro Pro Thr Asp 
                565                 570                 575     


Pro Leu Gly Ala Val Phe Ser Pro Pro Gln Ala Ser Pro Pro Gln Pro 
            580                 585                 590         


Ser His Gly Leu Gln Ser Cys Arg Asn Leu Arg Gly Ser Pro Lys Leu 
        595                 600                 605             


Pro Asp Phe Leu Gln Arg Asn Pro Leu Pro Pro Ile Leu Gly Ser Pro 
    610                 615                 620                 


Thr Lys Ala Val Pro Ser Phe Asp Phe Pro Lys Thr Pro Ser Ser Gln 
625                 630                 635                 640 


Asn Leu Leu Ala Leu Leu Ala Arg Gln Gly Val Val Met Thr Pro Pro 
                645                 650                 655     


Arg Asn Arg Thr Leu Pro Asp Leu Ser Glu Val Gly Pro Phe His Gly 
            660                 665                 670         


Gln Pro Leu Gly Pro Gly Leu Arg Pro Gly Glu Asp Pro Lys Gly Pro 
        675                 680                 685             


Phe Gly Arg Ser Phe Ser Thr Ser Arg Leu Thr Asp Leu Leu Leu Lys 
    690                 695                 700                 


Ala Ala Phe Gly Thr Gln Ala Pro Asp Pro Gly Ser Thr Glu Ser Leu 
705                 710                 715                 720 


Gln Glu Lys Pro Met Glu Ile Ala Pro Ser Ala Gly Phe Gly Gly Ser 
                725                 730                 735     


Leu His Pro Gly Ala Arg Ala Gly Gly Thr Ser Ser Pro Ser Pro Val 
            740                 745                 750         


Val Phe Thr Val Gly Ser Pro Pro Ser Gly Ser Thr Pro Pro Gln Gly 
        755                 760                 765             


Pro Arg Thr Arg Met Phe Ser Ala Gly Pro Thr Gly Ser Ala Ser Ser 
    770                 775                 780                 


Ser Ala Arg His Leu Val Pro Gly Pro Cys Ser Glu Ala Pro Ala Pro 
785                 790                 795                 800 


Glu Leu Pro Ala Pro Gly His Gly Cys Ser Phe Ala Asp Pro Ile Thr 
                805                 810                 815     


Ala Asn Leu Glu Gly Ala Val Thr Phe Glu Ala Pro Asp Leu Pro Glu 
            820                 825                 830         


Glu Thr Leu Met Glu Gln Glu His Thr Glu Ile Leu Arg Gly Leu Arg 
        835                 840                 845             


Phe Thr Leu Leu Phe Val Gln His Val Leu Glu Ile Ala Ala Leu Lys 
    850                 855                 860                 


Gly Ser Ala Ser Glu Ala Ala Gly Gly Pro Glu Tyr Gln Leu Gln Glu 
865                 870                 875                 880 


Ser Val Val Ala Asp Gln Ile Ser Leu Leu Ser Arg Glu Trp Gly Phe 
                885                 890                 895     


Ala Glu Gln Leu Val Leu Tyr Leu Lys Val Ala Glu Leu Leu Ser Ser 
            900                 905                 910         


Gly Leu Gln Ser Ala Ile Asp Gln Ile Arg Ala Gly Lys Leu Cys Leu 
        915                 920                 925             


Ser Ser Thr Val Lys Gln Val Val Arg Arg Leu Asn Glu Leu Tyr Lys 
    930                 935                 940                 


Ala Ser Val Val Ser Cys Gln Gly Leu Ser Leu Arg Leu Gln Arg Phe 
945                 950                 955                 960 


Phe Leu Asp Lys Gln Arg Leu Leu Asp Arg Ile His Ser Ile Thr Ala 
                965                 970                 975     


Glu Arg Leu Ile Phe Ser His Ala Val Gln Met Val Gln Ser Ala Ala 
            980                 985                 990         


Leu Asp Glu Met Phe Gln His Arg  Glu Gly Cys Val Pro  Arg Tyr His 
        995                 1000                 1005             


Lys Ala  Leu Leu Leu Leu Glu  Gly Leu Gln His Met  Leu Ser Asp 
    1010                 1015                 1020             


Gln Ala  Asp Ile Glu Asn Val  Thr Lys Cys Lys Leu  Cys Ile Glu 
    1025                 1030                 1035             


Arg Arg  Leu Ser Ala Leu Leu  Thr Gly Ile Cys Ala  
    1040                 1045                 1050 


<210> 3
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 3
accgcggcgg cacagagacc g                                                 21


<210> 4
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 4
accgccacgg cgccttcgcg g                                                 21


<210> 5
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 5
agaccaaagc gaaggcgccg                                                   20


<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<221> source
<223> /note="Description of Artificial Sequence: Synthetic
      oligonucleotide"

<400> 6
cggcccggga tcccccgccc                                                   20


