                         SEQUENCE LISTING

<110>  LABORATOIRE FRANCAIS DU FRACTIONNEMENT ET DES 
       BIOTECHNOLOGIES
       UNIVERSITE DE BORDEAUX
       CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
 
<120>  A specific binding molecule directed against galectin-3 protein.

<130>  WOB V17LFBATH

<150>  EP17306337.1
<151>  2017-10-05

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH / specific binding molecule

<400>  1

Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Asp Ser Val Ser Ser Asn 
            20                  25                  30          


Asn Phe Gly Trp Asn Trp Ile Arg Gln Ser Pro Ser Arg Gly Leu Glu 
        35                  40                  45              


Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys Trp Tyr Asn Asp Tyr Ala 
    50                  55                  60                  


Val Ser Val Arg Ser Arg Ile Thr Ile Asn Pro Asp Thr Ser Lys Asn 
65                  70                  75                  80  


Gln Phe Ser Leu Gln Leu Asn Ser Val Thr Pro Glu Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Gln Gly Ser Thr Tyr Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser 
        115                 120 


<210>  2
<211>  107
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL / specific binding molecule

<400>  2

Asp Ile Val Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      


Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Thr Ile Ser Ser Ser 
            20                  25                  30          


Leu Ala Trp Phe Gln Gln Arg Pro Gly Glu Ala Pro Asn Leu Leu Ile 
        35                  40                  45              


Tyr Ser Ala Ser Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  


Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  


Glu Asp Phe Ala Thr Tyr Ser Cys Gln Gln Thr Tyr Ser Ala Pro Pro 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105         


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH / CDR1

<400>  3

Gly Asp Ser Val Ser Ser Asn Asn Phe Gly 
1               5                   10  


<210>  4
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH / CDR2

<400>  4

Thr Tyr Tyr Arg Ser Lys Trp Tyr Asn 
1               5                   


<210>  5
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH / CDR3

<400>  5

Ala Arg Gln Gly Ser Thr Tyr Phe Asp Tyr 
1               5                   10  


<210>  6
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL / CDR1

<400>  6

Gln Thr Ile Ser Ser Ser 
1               5       


<210>  7
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL / CDR2

<400>  7

Ser Ala Ser  
1               


<210>  8
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL / CDR3

<400>  8

Gln Gln Thr Tyr Ser Ala Pro Pro Thr 
1               5                   


<210>  9
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Peptide linker

<400>  9

Glu Phe Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly 
1               5                   10                  15      


Ser Arg 
        


<210>  10
<211>  245
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of specific binding molecule

<400>  10

Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Lys Pro Ser Gln 
1               5                   10                  15      


Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Asp Ser Val Ser Ser Asn 
            20                  25                  30          


Asn Phe Gly Trp Asn Trp Ile Arg Gln Ser Pro Ser Arg Gly Leu Glu 
        35                  40                  45              


Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys Trp Tyr Asn Asp Tyr Ala 
    50                  55                  60                  


Val Ser Val Arg Ser Arg Ile Thr Ile Asn Pro Asp Thr Ser Lys Asn 
65                  70                  75                  80  


Gln Phe Ser Leu Gln Leu Asn Ser Val Thr Pro Glu Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Ala Arg Gln Gly Ser Thr Tyr Phe Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Leu Val Thr Val Ser Ser Glu Phe Gly Gly Gly Gly Ser Gly 
        115                 120                 125             


Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg Asp Ile Val Met Thr Gln 
    130                 135                 140                 


Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr Ile Thr 
145                 150                 155                 160 


Cys Arg Ala Ser Gln Thr Ile Ser Ser Ser Leu Ala Trp Phe Gln Gln 
                165                 170                 175     


Arg Pro Gly Glu Ala Pro Asn Leu Leu Ile Tyr Ser Ala Ser Ser Leu 
            180                 185                 190         


Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp 
        195                 200                 205             


Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala Thr Tyr 
    210                 215                 220                 


Ser Cys Gln Gln Thr Tyr Ser Ala Pro Pro Thr Phe Gly Gly Gly Thr 
225                 230                 235                 240 


Lys Leu Glu Ile Lys 
                245 


<210>  11
<211>  737
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of specific binding molecule

<400>  11
caggtacagc tgcagcagtc aggtccagga ctggtgaagc cctcgcagac cctctcactc       60

acctgtgcca tctccgggga cagtgtctct agcaacaatt ttggttggaa ctggatcagg      120

cagtccccat cgagaggcct tgagtggctg ggaaggacat actacaggtc caagtggtat      180

aatgattatg cagtatctgt gagaagtcga ataaccatca acccagacac atccaagaac      240

cagttctccc tgcagctgaa ctctgtgact cccgaggaca cggccgtgta ttactgtgca      300

agacagggca gcacttactt cgactattgg ggccagggca ccctggtcac tgtctcctca      360

gaattcggtg gcggtggctc gggcggtggt gggtctggtg gcggcggttc tagagacatc      420

gtgatgaccc agtctccatc ctccctgtct gcatctgtag gagacagagt caccatcact      480

tgccgggcaa gtcagaccat tagcagctct ttagcttggt ttcagcagag accgggagaa      540

gcccctaacc tcctgatcta tagtgcatcc agtttgcaaa gtggggtccc atcaaggttc      600

agtggcagtg gatctgggac agatttcact ctcaccatca gcagtcttca acctgaagat      660

tttgcaactt actcctgtca acagacttac agtgcccctc ccactttcgg cggagggacc      720

aagctggaga tcaaagt                                                     737


<210>  12
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Fc fragment of IgG1

<400>  12

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Lys 
225         


<210>  13
<211>  477
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Amino acid sequence of fusion protein

<400>  13

Gly Ser Gln Val Gln Leu Gln Gln Ser Gly Pro Gly Leu Val Lys Pro 
1               5                   10                  15      


Ser Gln Thr Leu Ser Leu Thr Cys Ala Ile Ser Gly Asp Ser Val Ser 
            20                  25                  30          


Ser Asn Asn Phe Gly Trp Asn Trp Ile Arg Gln Ser Pro Ser Arg Gly 
        35                  40                  45              


Leu Glu Trp Leu Gly Arg Thr Tyr Tyr Arg Ser Lys Trp Tyr Asn Asp 
    50                  55                  60                  


Tyr Ala Val Ser Val Arg Ser Arg Ile Thr Ile Asn Pro Asp Thr Ser 
65                  70                  75                  80  


Lys Asn Gln Phe Ser Leu Gln Leu Asn Ser Val Thr Pro Glu Asp Thr 
                85                  90                  95      


Ala Val Tyr Tyr Cys Ala Arg Gln Gly Ser Thr Tyr Phe Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Leu Val Thr Val Ser Ser Glu Phe Gly Gly Gly Gly 
        115                 120                 125             


Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Arg Asp Ile Val Met 
    130                 135                 140                 


Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly Asp Arg Val Thr 
145                 150                 155                 160 


Ile Thr Cys Arg Ala Ser Gln Thr Ile Ser Ser Ser Leu Ala Trp Phe 
                165                 170                 175     


Gln Gln Arg Pro Gly Glu Ala Pro Asn Leu Leu Ile Tyr Ser Ala Ser 
            180                 185                 190         


Ser Leu Gln Ser Gly Val Pro Ser Arg Phe Ser Gly Ser Gly Ser Gly 
        195                 200                 205             


Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Phe Ala 
    210                 215                 220                 


Thr Tyr Ser Cys Gln Gln Thr Tyr Ser Ala Pro Pro Thr Phe Gly Gly 
225                 230                 235                 240 


Gly Thr Lys Leu Glu Ile Lys Ala Ala Ala Asp Lys Thr His Thr Cys 
                245                 250                 255     


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
            260                 265                 270         


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
        275                 280                 285             


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
    290                 295                 300                 


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
305                 310                 315                 320 


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
                325                 330                 335     


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
            340                 345                 350         


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
        355                 360                 365             


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
    370                 375                 380                 


Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
385                 390                 395                 400 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
                405                 410                 415     


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
            420                 425                 430         


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
        435                 440                 445             


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
    450                 455                 460                 


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
465                 470                 475         


<210>  14
<211>  1447
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid sequence of fusion protein

<400>  14
cgccaacgcc ggatcccagg tgcagctgca gcagtctgga cccggcctcg tgaagcctag       60

ccagaccctg tctctgacct gcgccatcag cggcgatagc gtgtccagca acaacttcgg      120

ctggaactgg atcagacaga gccccagcag aggcctggaa tggctgggcc ggacctacta      180

ccggtccaag tggtacaacg actacgccgt gtccgtgcgg agccggatca ccatcaaccc      240

cgacaccagc aagaaccagt tctccctgca gctgaacagc gtgacccccg aggataccgc      300

cgtgtactac tgtgccagac agggcagcac ctacttcgac tactggggcc agggcaccct      360

cgtgaccgtg tctagcgaat ttggcggcgg aggatctggc ggaggcggaa gtggcggagg      420

gggctctaga gacatcgtga tgacccagag cccctccagc ctgtctgcca gcgtgggcga      480

cagagtgacc atcacctgta gggccagcca gaccatcagc agcagcctgg cctggtttca      540

gcagcggcca ggcgaagccc ccaacctgct gatctacagc gcctctagcc tgcagagcgg      600

cgtgcccagc agattttctg gcagcggctc cggcaccgac ttcaccctga caattagcag      660

cctgcagccc gaggacttcg ccacctacag ctgccagcag acctactccg cccctcctac      720

atttggaggc ggcaccaagc tggaaatcaa ggcggccgcg gacaaaactc acacatgccc      780

accgtgccca gcacctgaac tcctgggggg accgtcagtc ttcctcttcc ccccaaaacc      840

caaggacacc ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg tggacgtgag      900

ccacgaagac cctgaggtca agttcaactg gtacgtggac ggcgtggagg tgcataatgc      960

caagacaaag ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca gcgtcctcac     1020

cgtcctgcac caggactggc tgaatggcaa ggagtacaag tgcaaggtct ccaacaaagc     1080

cctcccagcc cccatcgaga aaaccatctc caaagccaaa gggcagcccc gagaaccaca     1140

ggtgtacacc ctgcccccat cccgggatga gctgaccaag aaccaggtca gcctgacctg     1200

cctggtcaaa ggcttctatc ccagcgacat cgccgtggag tgggagagca atgggcagcc     1260

ggagaacaac tacaagacca cgcctcccgt gctggactcc gacggctcct tcttcctcta     1320

tagcaagctc accgtggaca agagcaggtg gcagcagggg aacgtcttct catgctccgt     1380

gatgcatgag gctctgcaca accactacac gcagaagagc ctctccctgt ctccgggtaa     1440

atgatga                                                               1447


<210>  15
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Suitable peptide tag

<400>  15

His His His His His His 
1               5       


<210>  16
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Suitable peptide tag

<400>  16

Glu Asp Gln Val Asp Pro Arg Leu Ile Asp Gly Lys 
1               5                   10          


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Suitable peptide tag

<400>  17

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  18
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Suitable peptide tag

<400>  18

Gly Lys Pro Ile Pro Asn Pro Leu Leu Gly Leu Asp Ser Thr 
1               5                   10                  


<210>  19
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Suitable peptide tag

<400>  19

Ile Pro Asn Pro Leu Leu Gly Leu Asp 
1               5                   


<210>  20
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Suitable peptide tag

<400>  20

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  21
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Suitable peptide tag

<400>  21

Gly Gly Gly Gly Ser Leu Pro Glu Thr Gly Gly Ala Ala 
1               5                   10              


<210>  22
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Suitable peptide tag

<400>  22

Gly Gly Gly Gly Ser Cys Cys Ala Ala 
1               5                   


<210>  23
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Suitable peptide tag

<400>  23

Pro Thr Leu Tyr Asn Val Ser Leu Val Met Ser Asp Thr Ala Gly Thr 
1               5                   10                  15      


Cys Tyr 
        


<210>  24
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Suitable peptide spacer

<400>  24
ggtggcggtg gctcgggcgg tggtgggtct ggtggcggcg gt                          42


<210>  25
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  25
gctacttaag ggtgtccagt gccaggtgca gctgcagcag tctggacccg g                51


<210>  26
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  26
gctacgtacg cttgatttcc agcttggtgc cgcct                                  35


<210>  27
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P1-VH-C3

<400>  27
cagaggagag ctagcgaagc ttgccgccac catgcgatgg agctggatct tcctgctgct       60

gctgagcatc accagcgcca acgcccaggt gcagctgcag cagtctgga                  109


<210>  28
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2-VH-C3

<400>  28
gctcgcggcc gcactcaccg ctagacacgg tcacgagggt gccct                       45


<210>  29
<211>  107
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P1-VL-C3

<400>  29
ttccatttca gactagtaag cttgccgcca ccatgcgatg gagctggatc ttcctgctgc       60

tgctgagcat caccagcgcc aacgccgaca tcgtgatgac ccagagc                    107


<210>  30
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2-VL-C3

<400>  30
gccgcaaagt gcacttacgc ttgatttcca gcttggtgcc g                           41


<210>  31
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ch-Int 5'

<400>  31
gtgagtgcgg ccgcgagc                                                     18


<210>  32
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CH-Int 3'

<400>  32
gatcctcggc gcgcctcatc atttacccgg                                        30


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CK-Int 5'

<400>  33
gtaagtgcac tttgcggccg                                                   20


<210>  34
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CKI-XbaI-5'

<400>  34
atcagcgagc tctagactat caacactctc ccctgttgaa gct                         43


<210>  35
<211>  105
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P1-VL-C3-2

<400>  35
cagaggagag ctagcaagct tgccgccacc atgcgatgga gctggatctt cctgctgctg       60

ctgagcatca ccagcgccaa cgccgacatc gtgatgaccc agagc                      105


<210>  36
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2-VL-C3-2

<400>  36
gccgcaaagt gcacttacgc ttgatttcca gcttggtgcc g                           41


<210>  37
<211>  109
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P1-VH-C3-2

<400>  37
ccatttcaga ctagtgaagc ttgccgccac catgcgatgg agctggatct tcctgctgct       60

gctgagcatc accagcgcca acgcccaggt gcagctgcag cagtctgga                  109


<210>  38
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2-VH-C3-2

<400>  38
gctcgcggcc gcactcacgc tagacacggt cacgagggtg ccct                        44


<210>  39
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'CH-xba

<400>  39
aggctgatca gcgagctcta gatcatcatt tacccggaga caggga                      46


<210>  40
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  3'CK-asc

<400>  40
gatcctcggc gcgccctatc aacactctcc cctgttgaag ct                          42


<210>  41
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P1-MB7-V4

<400>  41
tttcctctcc tgacagctag cgaagcttgc cgccaccatg cgatggagct ggatcttcct       60


<210>  42
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2-WT-HI

<400>  42
cttgccggcc tcgagtcatc atttacccgg agacaggga                              39


<210>  43
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P2-WT-KI

<400>  43
cttgccggcc tcgagctatc aacactctcc cctgttgaag ct                          42


