                         SEQUENCE LISTING

<110>  Novozymes A/S
 
<120>  POLYPEPTIDES HAVING MANNANASE ACTIVITY AND POLYNUCLEOTIDES 
       ENCODING SAME

<130>  14623-WO-PCT

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  1587
<212>  DNA
<213>  Paenibacillus woosongensis


<220>
<221>  CDS
<222>  (1)..(1584)

<220>
<221>  sig_peptide
<222>  (1)..(90)

<220>
<221>  mat_peptide
<222>  (91)..(1584)

<400>  1
atg gtt ttg tta tgc atg ata att ctg atg gga atg tct ggt tca gct         48
Met Val Leu Leu Cys Met Ile Ile Leu Met Gly Met Ser Gly Ser Ala           
-30                 -25                 -20                 -15           

gct tca ata gcc gat gct tct tca acc gga caa gtt gct ctc atg aat         96
Ala Ser Ile Ala Asp Ala Ser Ser Thr Gly Gln Val Ala Leu Met Asn           
                -10                 -5              -1  1                 

atg gaa ggc acg cca tct gtc agt ccg act aac agc ata acg gtt act        144
Met Glu Gly Thr Pro Ser Val Ser Pro Thr Asn Ser Ile Thr Val Thr           
        5                   10                  15                        

ttt gct aat gcg gtg tta gaa ggt tac ggt atc gag aaa cgc ggt tct        192
Phe Ala Asn Ala Val Leu Glu Gly Tyr Gly Ile Glu Lys Arg Gly Ser           
    20                  25                  30                            

gtc aaa gaa gac gat gat act ttg tat gac ggt gaa ggc tat atc tct        240
Val Lys Glu Asp Asp Asp Thr Leu Tyr Asp Gly Glu Gly Tyr Ile Ser           
35                  40                  45                  50            

tac ttt ttt gat gaa att gga ggc gct gca gaa ccc gtc ggc agt gca        288
Tyr Phe Phe Asp Glu Ile Gly Gly Ala Ala Glu Pro Val Gly Ser Ala           
                55                  60                  65                

gct ttt act gtg gac gct gcg aaa gct ggg ctg tat gag ctg agt tta        336
Ala Phe Thr Val Asp Ala Ala Lys Ala Gly Leu Tyr Glu Leu Ser Leu           
            70                  75                  80                    

ggc tac tac atc ccc gaa ggc tac ggg gat aaa gtg acc cgt ata cag        384
Gly Tyr Tyr Ile Pro Glu Gly Tyr Gly Asp Lys Val Thr Arg Ile Gln           
        85                  90                  95                        

att aat ggt gaa ggc acc gga gag ctg aca ttg gat gcg ccg gca gca        432
Ile Asn Gly Glu Gly Thr Gly Glu Leu Thr Leu Asp Ala Pro Ala Ala           
    100                 105                 110                           

ggt acg gtt cgt gct gag aaa atg gtc agt aag gtg ctg ctg aac gca        480
Gly Thr Val Arg Ala Glu Lys Met Val Ser Lys Val Leu Leu Asn Ala           
115                 120                 125                 130           

ggc agc aat aca atc caa att atg cgc gga tgg ggt tac tac ggc att        528
Gly Ser Asn Thr Ile Gln Ile Met Arg Gly Trp Gly Tyr Tyr Gly Ile           
                135                 140                 145               

gag cat atc aag ctt gca ccc gcg aat gaa gca cca ccc agt aac aag        576
Glu His Ile Lys Leu Ala Pro Ala Asn Glu Ala Pro Pro Ser Asn Lys           
            150                 155                 160                   

ctg aat gca gag gac agc atc agg act ggc aca ttg aac aat ccc gaa        624
Leu Asn Ala Glu Asp Ser Ile Arg Thr Gly Thr Leu Asn Asn Pro Glu           
        165                 170                 175                       

gcg aca gct gag gcc aga gcg cta atg aac tac ttg ctc agc cag tat        672
Ala Thr Ala Glu Ala Arg Ala Leu Met Asn Tyr Leu Leu Ser Gln Tyr           
    180                 185                 190                           

gga caa aaa att atc tct gga cag cag acg ctt gaa gat gtg gag tgg        720
Gly Gln Lys Ile Ile Ser Gly Gln Gln Thr Leu Glu Asp Val Glu Trp           
195                 200                 205                 210           

atc aag cag cag aca ggc aaa tat cca gcg att ttc tct aca gac ttg        768
Ile Lys Gln Gln Thr Gly Lys Tyr Pro Ala Ile Phe Ser Thr Asp Leu           
                215                 220                 225               

atg gat tac tcc cct tcc cgc gtg gat cat gga gcc tcc tcc act gag        816
Met Asp Tyr Ser Pro Ser Arg Val Asp His Gly Ala Ser Ser Thr Glu           
            230                 235                 240                   

gtc gag aag atg atc gaa tgg tac aaa cgc ggt ggt att gtg tct tta        864
Val Glu Lys Met Ile Glu Trp Tyr Lys Arg Gly Gly Ile Val Ser Leu           
        245                 250                 255                       

tgc tgg cac tgg aat gcc ccg aag gga atc ggc ggc aat gag cct ggc        912
Cys Trp His Trp Asn Ala Pro Lys Gly Ile Gly Gly Asn Glu Pro Gly           
    260                 265                 270                           

aac gag tgg tgg cga ggc ttc tac act gaa ttt aca acc ttt gat gtg        960
Asn Glu Trp Trp Arg Gly Phe Tyr Thr Glu Phe Thr Thr Phe Asp Val           
275                 280                 285                 290           

gaa tat gct ctt aat cat ccg gat tct gag gac tac cag ctc ctg atc       1008
Glu Tyr Ala Leu Asn His Pro Asp Ser Glu Asp Tyr Gln Leu Leu Ile           
                295                 300                 305               

cgg gac att gac gcc atc gca gtt cag ttg aag cga ttg cag gag gcg       1056
Arg Asp Ile Asp Ala Ile Ala Val Gln Leu Lys Arg Leu Gln Glu Ala           
            310                 315                 320                   

aac gtg cct gtg tta tgg cga ccc ctg cac gag gca gag ggc acc tgg       1104
Asn Val Pro Val Leu Trp Arg Pro Leu His Glu Ala Glu Gly Thr Trp           
        325                 330                 335                       

ttt tgg tgg gga gca aaa ggg ccc gag ccg gcg aaa cag ctc tat cgt       1152
Phe Trp Trp Gly Ala Lys Gly Pro Glu Pro Ala Lys Gln Leu Tyr Arg           
    340                 345                 350                           

tta atg tat gat cgg tta acc aat gat cat aag ctg aac aat ctg att       1200
Leu Met Tyr Asp Arg Leu Thr Asn Asp His Lys Leu Asn Asn Leu Ile           
355                 360                 365                 370           

tgg gtg tgg aac tcc gag aaa aag gat tgg tat ccg gga gat gat gtc       1248
Trp Val Trp Asn Ser Glu Lys Lys Asp Trp Tyr Pro Gly Asp Asp Val           
                375                 380                 385               

gta gat atg gta agc gtt gat atc tac aac cct gca ggc gac tat aat       1296
Val Asp Met Val Ser Val Asp Ile Tyr Asn Pro Ala Gly Asp Tyr Asn           
            390                 395                 400                   

ccg agc atc gca aaa tat gaa gcg ctt gta tct ttg gcg gac aac aag       1344
Pro Ser Ile Ala Lys Tyr Glu Ala Leu Val Ser Leu Ala Asp Asn Lys           
        405                 410                 415                       

aag atg gct gca cta gcg gag aat ggg cct att ccg gat ccg gat gct       1392
Lys Met Ala Ala Leu Ala Glu Asn Gly Pro Ile Pro Asp Pro Asp Ala           
    420                 425                 430                           

ctt cag gag tac ggc gcc gac tgg agc ttc ttt agt acc tgg acc ggc       1440
Leu Gln Glu Tyr Gly Ala Asp Trp Ser Phe Phe Ser Thr Trp Thr Gly           
435                 440                 445                 450           

gac tac atc agg gat ggc aag aca aat acg ata gaa cat ttg aag aag       1488
Asp Tyr Ile Arg Asp Gly Lys Thr Asn Thr Ile Glu His Leu Lys Lys           
                455                 460                 465               

gta tat caa cac gat tac gtc att act ctc gac gaa ctc ccg gca gac       1536
Val Tyr Gln His Asp Tyr Val Ile Thr Leu Asp Glu Leu Pro Ala Asp           
            470                 475                 480                   

tgt act cca atc ttg atg ata agg caa aga atg gtg aat cag cag gga       1584
Cys Thr Pro Ile Leu Met Ile Arg Gln Arg Met Val Asn Gln Gln Gly           
        485                 490                 495                       

tga                                                                   1587


<210>  2
<211>  528
<212>  PRT
<213>  Paenibacillus woosongensis

<400>  2

Met Val Leu Leu Cys Met Ile Ile Leu Met Gly Met Ser Gly Ser Ala 
-30                 -25                 -20                 -15 


Ala Ser Ile Ala Asp Ala Ser Ser Thr Gly Gln Val Ala Leu Met Asn 
                -10                 -5              -1  1       


Met Glu Gly Thr Pro Ser Val Ser Pro Thr Asn Ser Ile Thr Val Thr 
        5                   10                  15              


Phe Ala Asn Ala Val Leu Glu Gly Tyr Gly Ile Glu Lys Arg Gly Ser 
    20                  25                  30                  


Val Lys Glu Asp Asp Asp Thr Leu Tyr Asp Gly Glu Gly Tyr Ile Ser 
35                  40                  45                  50  


Tyr Phe Phe Asp Glu Ile Gly Gly Ala Ala Glu Pro Val Gly Ser Ala 
                55                  60                  65      


Ala Phe Thr Val Asp Ala Ala Lys Ala Gly Leu Tyr Glu Leu Ser Leu 
            70                  75                  80          


Gly Tyr Tyr Ile Pro Glu Gly Tyr Gly Asp Lys Val Thr Arg Ile Gln 
        85                  90                  95              


Ile Asn Gly Glu Gly Thr Gly Glu Leu Thr Leu Asp Ala Pro Ala Ala 
    100                 105                 110                 


Gly Thr Val Arg Ala Glu Lys Met Val Ser Lys Val Leu Leu Asn Ala 
115                 120                 125                 130 


Gly Ser Asn Thr Ile Gln Ile Met Arg Gly Trp Gly Tyr Tyr Gly Ile 
                135                 140                 145     


Glu His Ile Lys Leu Ala Pro Ala Asn Glu Ala Pro Pro Ser Asn Lys 
            150                 155                 160         


Leu Asn Ala Glu Asp Ser Ile Arg Thr Gly Thr Leu Asn Asn Pro Glu 
        165                 170                 175             


Ala Thr Ala Glu Ala Arg Ala Leu Met Asn Tyr Leu Leu Ser Gln Tyr 
    180                 185                 190                 


Gly Gln Lys Ile Ile Ser Gly Gln Gln Thr Leu Glu Asp Val Glu Trp 
195                 200                 205                 210 


Ile Lys Gln Gln Thr Gly Lys Tyr Pro Ala Ile Phe Ser Thr Asp Leu 
                215                 220                 225     


Met Asp Tyr Ser Pro Ser Arg Val Asp His Gly Ala Ser Ser Thr Glu 
            230                 235                 240         


Val Glu Lys Met Ile Glu Trp Tyr Lys Arg Gly Gly Ile Val Ser Leu 
        245                 250                 255             


Cys Trp His Trp Asn Ala Pro Lys Gly Ile Gly Gly Asn Glu Pro Gly 
    260                 265                 270                 


Asn Glu Trp Trp Arg Gly Phe Tyr Thr Glu Phe Thr Thr Phe Asp Val 
275                 280                 285                 290 


Glu Tyr Ala Leu Asn His Pro Asp Ser Glu Asp Tyr Gln Leu Leu Ile 
                295                 300                 305     


Arg Asp Ile Asp Ala Ile Ala Val Gln Leu Lys Arg Leu Gln Glu Ala 
            310                 315                 320         


Asn Val Pro Val Leu Trp Arg Pro Leu His Glu Ala Glu Gly Thr Trp 
        325                 330                 335             


Phe Trp Trp Gly Ala Lys Gly Pro Glu Pro Ala Lys Gln Leu Tyr Arg 
    340                 345                 350                 


Leu Met Tyr Asp Arg Leu Thr Asn Asp His Lys Leu Asn Asn Leu Ile 
355                 360                 365                 370 


Trp Val Trp Asn Ser Glu Lys Lys Asp Trp Tyr Pro Gly Asp Asp Val 
                375                 380                 385     


Val Asp Met Val Ser Val Asp Ile Tyr Asn Pro Ala Gly Asp Tyr Asn 
            390                 395                 400         


Pro Ser Ile Ala Lys Tyr Glu Ala Leu Val Ser Leu Ala Asp Asn Lys 
        405                 410                 415             


Lys Met Ala Ala Leu Ala Glu Asn Gly Pro Ile Pro Asp Pro Asp Ala 
    420                 425                 430                 


Leu Gln Glu Tyr Gly Ala Asp Trp Ser Phe Phe Ser Thr Trp Thr Gly 
435                 440                 445                 450 


Asp Tyr Ile Arg Asp Gly Lys Thr Asn Thr Ile Glu His Leu Lys Lys 
                455                 460                 465     


Val Tyr Gln His Asp Tyr Val Ile Thr Leu Asp Glu Leu Pro Ala Asp 
            470                 475                 480         


Cys Thr Pro Ile Leu Met Ile Arg Gln Arg Met Val Asn Gln Gln Gly 
        485                 490                 495             


<210>  3
<211>  498
<212>  PRT
<213>  Paenibacillus woosongensis


<220>
<221>  mat_peptide
<222>  (1)..(498)

<400>  3

Met Asn Met Glu Gly Thr Pro Ser Val Ser Pro Thr Asn Ser Ile Thr 
1               5                   10                  15      


Val Thr Phe Ala Asn Ala Val Leu Glu Gly Tyr Gly Ile Glu Lys Arg 
            20                  25                  30          


Gly Ser Val Lys Glu Asp Asp Asp Thr Leu Tyr Asp Gly Glu Gly Tyr 
        35                  40                  45              


Ile Ser Tyr Phe Phe Asp Glu Ile Gly Gly Ala Ala Glu Pro Val Gly 
    50                  55                  60                  


Ser Ala Ala Phe Thr Val Asp Ala Ala Lys Ala Gly Leu Tyr Glu Leu 
65                  70                  75                  80  


Ser Leu Gly Tyr Tyr Ile Pro Glu Gly Tyr Gly Asp Lys Val Thr Arg 
                85                  90                  95      


Ile Gln Ile Asn Gly Glu Gly Thr Gly Glu Leu Thr Leu Asp Ala Pro 
            100                 105                 110         


Ala Ala Gly Thr Val Arg Ala Glu Lys Met Val Ser Lys Val Leu Leu 
        115                 120                 125             


Asn Ala Gly Ser Asn Thr Ile Gln Ile Met Arg Gly Trp Gly Tyr Tyr 
    130                 135                 140                 


Gly Ile Glu His Ile Lys Leu Ala Pro Ala Asn Glu Ala Pro Pro Ser 
145                 150                 155                 160 


Asn Lys Leu Asn Ala Glu Asp Ser Ile Arg Thr Gly Thr Leu Asn Asn 
                165                 170                 175     


Pro Glu Ala Thr Ala Glu Ala Arg Ala Leu Met Asn Tyr Leu Leu Ser 
            180                 185                 190         


Gln Tyr Gly Gln Lys Ile Ile Ser Gly Gln Gln Thr Leu Glu Asp Val 
        195                 200                 205             


Glu Trp Ile Lys Gln Gln Thr Gly Lys Tyr Pro Ala Ile Phe Ser Thr 
    210                 215                 220                 


Asp Leu Met Asp Tyr Ser Pro Ser Arg Val Asp His Gly Ala Ser Ser 
225                 230                 235                 240 


Thr Glu Val Glu Lys Met Ile Glu Trp Tyr Lys Arg Gly Gly Ile Val 
                245                 250                 255     


Ser Leu Cys Trp His Trp Asn Ala Pro Lys Gly Ile Gly Gly Asn Glu 
            260                 265                 270         


Pro Gly Asn Glu Trp Trp Arg Gly Phe Tyr Thr Glu Phe Thr Thr Phe 
        275                 280                 285             


Asp Val Glu Tyr Ala Leu Asn His Pro Asp Ser Glu Asp Tyr Gln Leu 
    290                 295                 300                 


Leu Ile Arg Asp Ile Asp Ala Ile Ala Val Gln Leu Lys Arg Leu Gln 
305                 310                 315                 320 


Glu Ala Asn Val Pro Val Leu Trp Arg Pro Leu His Glu Ala Glu Gly 
                325                 330                 335     


Thr Trp Phe Trp Trp Gly Ala Lys Gly Pro Glu Pro Ala Lys Gln Leu 
            340                 345                 350         


Tyr Arg Leu Met Tyr Asp Arg Leu Thr Asn Asp His Lys Leu Asn Asn 
        355                 360                 365             


Leu Ile Trp Val Trp Asn Ser Glu Lys Lys Asp Trp Tyr Pro Gly Asp 
    370                 375                 380                 


Asp Val Val Asp Met Val Ser Val Asp Ile Tyr Asn Pro Ala Gly Asp 
385                 390                 395                 400 


Tyr Asn Pro Ser Ile Ala Lys Tyr Glu Ala Leu Val Ser Leu Ala Asp 
                405                 410                 415     


Asn Lys Lys Met Ala Ala Leu Ala Glu Asn Gly Pro Ile Pro Asp Pro 
            420                 425                 430         


Asp Ala Leu Gln Glu Tyr Gly Ala Asp Trp Ser Phe Phe Ser Thr Trp 
        435                 440                 445             


Thr Gly Asp Tyr Ile Arg Asp Gly Lys Thr Asn Thr Ile Glu His Leu 
    450                 455                 460                 


Lys Lys Val Tyr Gln His Asp Tyr Val Ile Thr Leu Asp Glu Leu Pro 
465                 470                 475                 480 


Ala Asp Cys Thr Pro Ile Leu Met Ile Arg Gln Arg Met Val Asn Gln 
                485                 490                 495     


Gln Gly 
        


<210>  4
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Truncated GH26 mannanase isolated from Paenibacillus woosongensis
       wild type missing the CBM35 domain


<220>
<221>  mat_peptide
<222>  (1)..(330)

<400>  4

Ile Arg Thr Gly Thr Leu Asn Asn Pro Glu Ala Thr Ala Glu Ala Arg 
1               5                   10                  15      


Ala Leu Met Asn Tyr Leu Leu Ser Gln Tyr Gly Gln Lys Ile Ile Ser 
            20                  25                  30          


Gly Gln Gln Thr Leu Glu Asp Val Glu Trp Ile Lys Gln Gln Thr Gly 
        35                  40                  45              


Lys Tyr Pro Ala Ile Phe Ser Thr Asp Leu Met Asp Tyr Ser Pro Ser 
    50                  55                  60                  


Arg Val Asp His Gly Ala Ser Ser Thr Glu Val Glu Lys Met Ile Glu 
65                  70                  75                  80  


Trp Tyr Lys Arg Gly Gly Ile Val Ser Leu Cys Trp His Trp Asn Ala 
                85                  90                  95      


Pro Lys Gly Ile Gly Gly Asn Glu Pro Gly Asn Glu Trp Trp Arg Gly 
            100                 105                 110         


Phe Tyr Thr Glu Phe Thr Thr Phe Asp Val Glu Tyr Ala Leu Asn His 
        115                 120                 125             


Pro Asp Ser Glu Asp Tyr Gln Leu Leu Ile Arg Asp Ile Asp Ala Ile 
    130                 135                 140                 


Ala Val Gln Leu Lys Arg Leu Gln Glu Ala Asn Val Pro Val Leu Trp 
145                 150                 155                 160 


Arg Pro Leu His Glu Ala Glu Gly Thr Trp Phe Trp Trp Gly Ala Lys 
                165                 170                 175     


Gly Pro Glu Pro Ala Lys Gln Leu Tyr Arg Leu Met Tyr Asp Arg Leu 
            180                 185                 190         


Thr Asn Asp His Lys Leu Asn Asn Leu Ile Trp Val Trp Asn Ser Glu 
        195                 200                 205             


Lys Lys Asp Trp Tyr Pro Gly Asp Asp Val Val Asp Met Val Ser Val 
    210                 215                 220                 


Asp Ile Tyr Asn Pro Ala Gly Asp Tyr Asn Pro Ser Ile Ala Lys Tyr 
225                 230                 235                 240 


Glu Ala Leu Val Ser Leu Ala Asp Asn Lys Lys Met Ala Ala Leu Ala 
                245                 250                 255     


Glu Asn Gly Pro Ile Pro Asp Pro Asp Ala Leu Gln Glu Tyr Gly Ala 
            260                 265                 270         


Asp Trp Ser Phe Phe Ser Thr Trp Thr Gly Asp Tyr Ile Arg Asp Gly 
        275                 280                 285             


Lys Thr Asn Thr Ile Glu His Leu Lys Lys Val Tyr Gln His Asp Tyr 
    290                 295                 300                 


Val Ile Thr Leu Asp Glu Leu Pro Ala Asp Cys Thr Pro Ile Leu Met 
305                 310                 315                 320 


Ile Arg Gln Arg Met Val Asn Gln Gln Gly 
                325                 330 


<210>  5
<211>  1515
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct DNA sequence of the GH26 mannanase comprising a CBM35 
       wherein the native secretion signal was removed and a 6xHis tag 
       was added directly on the C-terminal of the protein


<220>
<221>  CDS
<222>  (1)..(1512)

<220>
<221>  mat_peptide
<222>  (1)..(1512)

<400>  5
atg aat atg gaa ggc acg cca tct gtc agt ccg act aac agc ata acg         48
Met Asn Met Glu Gly Thr Pro Ser Val Ser Pro Thr Asn Ser Ile Thr           
1               5                   10                  15                

gtt act ttt gct aat gcg gtg tta gaa ggt tac ggt atc gag aaa cgc         96
Val Thr Phe Ala Asn Ala Val Leu Glu Gly Tyr Gly Ile Glu Lys Arg           
            20                  25                  30                    

ggt tct gtc aaa gaa gac gat gat act ttg tat gac ggt gaa ggc tat        144
Gly Ser Val Lys Glu Asp Asp Asp Thr Leu Tyr Asp Gly Glu Gly Tyr           
        35                  40                  45                        

atc tct tac ttt ttt gat gaa att gga ggc gct gca gaa ccc gtc ggc        192
Ile Ser Tyr Phe Phe Asp Glu Ile Gly Gly Ala Ala Glu Pro Val Gly           
    50                  55                  60                            

agt gca gct ttt act gtg gac gct gcg aaa gct ggg ctg tat gag ctg        240
Ser Ala Ala Phe Thr Val Asp Ala Ala Lys Ala Gly Leu Tyr Glu Leu           
65                  70                  75                  80            

agt tta ggc tac tac atc ccc gaa ggc tac ggg gat aaa gtg acc cgt        288
Ser Leu Gly Tyr Tyr Ile Pro Glu Gly Tyr Gly Asp Lys Val Thr Arg           
                85                  90                  95                

ata cag att aat ggt gaa ggc acc gga gag ctg aca ttg gat gcg ccg        336
Ile Gln Ile Asn Gly Glu Gly Thr Gly Glu Leu Thr Leu Asp Ala Pro           
            100                 105                 110                   

gca gca ggt acg gtt cgt gct gag aaa atg gtc agt aag gtg ctg ctg        384
Ala Ala Gly Thr Val Arg Ala Glu Lys Met Val Ser Lys Val Leu Leu           
        115                 120                 125                       

aac gca ggc agc aat aca atc caa att atg cgc gga tgg ggt tac tac        432
Asn Ala Gly Ser Asn Thr Ile Gln Ile Met Arg Gly Trp Gly Tyr Tyr           
    130                 135                 140                           

ggc att gag cat atc aag ctt gca ccc gcg aat gaa gca cca ccc agt        480
Gly Ile Glu His Ile Lys Leu Ala Pro Ala Asn Glu Ala Pro Pro Ser           
145                 150                 155                 160           

aac aag ctg aat gca gag gac agc atc agg act ggc aca ttg aac aat        528
Asn Lys Leu Asn Ala Glu Asp Ser Ile Arg Thr Gly Thr Leu Asn Asn           
                165                 170                 175               

ccc gaa gcg aca gct gag gcc aga gcg cta atg aac tac ttg ctc agc        576
Pro Glu Ala Thr Ala Glu Ala Arg Ala Leu Met Asn Tyr Leu Leu Ser           
            180                 185                 190                   

cag tat gga caa aaa att atc tct gga cag cag acg ctt gaa gat gtg        624
Gln Tyr Gly Gln Lys Ile Ile Ser Gly Gln Gln Thr Leu Glu Asp Val           
        195                 200                 205                       

gag tgg atc aag cag cag aca ggc aaa tat cca gcg att ttc tct aca        672
Glu Trp Ile Lys Gln Gln Thr Gly Lys Tyr Pro Ala Ile Phe Ser Thr           
    210                 215                 220                           

gac ttg atg gat tac tcc cct tcc cgc gtg gat cat gga gcc tcc tcc        720
Asp Leu Met Asp Tyr Ser Pro Ser Arg Val Asp His Gly Ala Ser Ser           
225                 230                 235                 240           

act gag gtc gag aag atg atc gaa tgg tac aaa cgc ggt ggt att gtg        768
Thr Glu Val Glu Lys Met Ile Glu Trp Tyr Lys Arg Gly Gly Ile Val           
                245                 250                 255               

tct tta tgc tgg cac tgg aat gcc ccg aag gga atc ggc ggc aat gag        816
Ser Leu Cys Trp His Trp Asn Ala Pro Lys Gly Ile Gly Gly Asn Glu           
            260                 265                 270                   

cct ggc aac gag tgg tgg cga ggc ttc tac act gaa ttt aca acc ttt        864
Pro Gly Asn Glu Trp Trp Arg Gly Phe Tyr Thr Glu Phe Thr Thr Phe           
        275                 280                 285                       

gat gtg gaa tat gct ctt aat cat ccg gat tct gag gac tac cag ctc        912
Asp Val Glu Tyr Ala Leu Asn His Pro Asp Ser Glu Asp Tyr Gln Leu           
    290                 295                 300                           

ctg atc cgg gac att gac gcc atc gca gtt cag ttg aag cga ttg cag        960
Leu Ile Arg Asp Ile Asp Ala Ile Ala Val Gln Leu Lys Arg Leu Gln           
305                 310                 315                 320           

gag gcg aac gtg cct gtg tta tgg cga ccc ctg cac gag gca gag ggc       1008
Glu Ala Asn Val Pro Val Leu Trp Arg Pro Leu His Glu Ala Glu Gly           
                325                 330                 335               

acc tgg ttt tgg tgg gga gca aaa ggg ccc gag ccg gcg aaa cag ctc       1056
Thr Trp Phe Trp Trp Gly Ala Lys Gly Pro Glu Pro Ala Lys Gln Leu           
            340                 345                 350                   

tat cgt tta atg tat gat cgg tta acc aat gat cat aag ctg aac aat       1104
Tyr Arg Leu Met Tyr Asp Arg Leu Thr Asn Asp His Lys Leu Asn Asn           
        355                 360                 365                       

ctg att tgg gtg tgg aac tcc gag aaa aag gat tgg tat ccg gga gat       1152
Leu Ile Trp Val Trp Asn Ser Glu Lys Lys Asp Trp Tyr Pro Gly Asp           
    370                 375                 380                           

gat gtc gta gat atg gta agc gtt gat atc tac aac cct gca ggc gac       1200
Asp Val Val Asp Met Val Ser Val Asp Ile Tyr Asn Pro Ala Gly Asp           
385                 390                 395                 400           

tat aat ccg agc atc gca aaa tat gaa gcg ctt gta tct ttg gcg gac       1248
Tyr Asn Pro Ser Ile Ala Lys Tyr Glu Ala Leu Val Ser Leu Ala Asp           
                405                 410                 415               

aac aag aag atg gct gca cta gcg gag aat ggg cct att ccg gat ccg       1296
Asn Lys Lys Met Ala Ala Leu Ala Glu Asn Gly Pro Ile Pro Asp Pro           
            420                 425                 430                   

gat gct ctt cag gag tac ggc gcc gac tgg agc ttc ttt agt acc tgg       1344
Asp Ala Leu Gln Glu Tyr Gly Ala Asp Trp Ser Phe Phe Ser Thr Trp           
        435                 440                 445                       

acc ggc gac tac atc agg gat ggc aag aca aat acg ata gaa cat ttg       1392
Thr Gly Asp Tyr Ile Arg Asp Gly Lys Thr Asn Thr Ile Glu His Leu           
    450                 455                 460                           

aag aag gta tat caa cac gat tac gtc att act ctc gac gaa ctc ccg       1440
Lys Lys Val Tyr Gln His Asp Tyr Val Ile Thr Leu Asp Glu Leu Pro           
465                 470                 475                 480           

gca gac tgt act cca atc ttg atg ata agg caa aga atg gtg aat cag       1488
Ala Asp Cys Thr Pro Ile Leu Met Ile Arg Gln Arg Met Val Asn Gln           
                485                 490                 495               

cag gga cat cat cac cat cac cac taa                                   1515
Gln Gly His His His His His His                                           
            500                                                           


<210>  6
<211>  504
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  6

Met Asn Met Glu Gly Thr Pro Ser Val Ser Pro Thr Asn Ser Ile Thr 
1               5                   10                  15      


Val Thr Phe Ala Asn Ala Val Leu Glu Gly Tyr Gly Ile Glu Lys Arg 
            20                  25                  30          


Gly Ser Val Lys Glu Asp Asp Asp Thr Leu Tyr Asp Gly Glu Gly Tyr 
        35                  40                  45              


Ile Ser Tyr Phe Phe Asp Glu Ile Gly Gly Ala Ala Glu Pro Val Gly 
    50                  55                  60                  


Ser Ala Ala Phe Thr Val Asp Ala Ala Lys Ala Gly Leu Tyr Glu Leu 
65                  70                  75                  80  


Ser Leu Gly Tyr Tyr Ile Pro Glu Gly Tyr Gly Asp Lys Val Thr Arg 
                85                  90                  95      


Ile Gln Ile Asn Gly Glu Gly Thr Gly Glu Leu Thr Leu Asp Ala Pro 
            100                 105                 110         


Ala Ala Gly Thr Val Arg Ala Glu Lys Met Val Ser Lys Val Leu Leu 
        115                 120                 125             


Asn Ala Gly Ser Asn Thr Ile Gln Ile Met Arg Gly Trp Gly Tyr Tyr 
    130                 135                 140                 


Gly Ile Glu His Ile Lys Leu Ala Pro Ala Asn Glu Ala Pro Pro Ser 
145                 150                 155                 160 


Asn Lys Leu Asn Ala Glu Asp Ser Ile Arg Thr Gly Thr Leu Asn Asn 
                165                 170                 175     


Pro Glu Ala Thr Ala Glu Ala Arg Ala Leu Met Asn Tyr Leu Leu Ser 
            180                 185                 190         


Gln Tyr Gly Gln Lys Ile Ile Ser Gly Gln Gln Thr Leu Glu Asp Val 
        195                 200                 205             


Glu Trp Ile Lys Gln Gln Thr Gly Lys Tyr Pro Ala Ile Phe Ser Thr 
    210                 215                 220                 


Asp Leu Met Asp Tyr Ser Pro Ser Arg Val Asp His Gly Ala Ser Ser 
225                 230                 235                 240 


Thr Glu Val Glu Lys Met Ile Glu Trp Tyr Lys Arg Gly Gly Ile Val 
                245                 250                 255     


Ser Leu Cys Trp His Trp Asn Ala Pro Lys Gly Ile Gly Gly Asn Glu 
            260                 265                 270         


Pro Gly Asn Glu Trp Trp Arg Gly Phe Tyr Thr Glu Phe Thr Thr Phe 
        275                 280                 285             


Asp Val Glu Tyr Ala Leu Asn His Pro Asp Ser Glu Asp Tyr Gln Leu 
    290                 295                 300                 


Leu Ile Arg Asp Ile Asp Ala Ile Ala Val Gln Leu Lys Arg Leu Gln 
305                 310                 315                 320 


Glu Ala Asn Val Pro Val Leu Trp Arg Pro Leu His Glu Ala Glu Gly 
                325                 330                 335     


Thr Trp Phe Trp Trp Gly Ala Lys Gly Pro Glu Pro Ala Lys Gln Leu 
            340                 345                 350         


Tyr Arg Leu Met Tyr Asp Arg Leu Thr Asn Asp His Lys Leu Asn Asn 
        355                 360                 365             


Leu Ile Trp Val Trp Asn Ser Glu Lys Lys Asp Trp Tyr Pro Gly Asp 
    370                 375                 380                 


Asp Val Val Asp Met Val Ser Val Asp Ile Tyr Asn Pro Ala Gly Asp 
385                 390                 395                 400 


Tyr Asn Pro Ser Ile Ala Lys Tyr Glu Ala Leu Val Ser Leu Ala Asp 
                405                 410                 415     


Asn Lys Lys Met Ala Ala Leu Ala Glu Asn Gly Pro Ile Pro Asp Pro 
            420                 425                 430         


Asp Ala Leu Gln Glu Tyr Gly Ala Asp Trp Ser Phe Phe Ser Thr Trp 
        435                 440                 445             


Thr Gly Asp Tyr Ile Arg Asp Gly Lys Thr Asn Thr Ile Glu His Leu 
    450                 455                 460                 


Lys Lys Val Tyr Gln His Asp Tyr Val Ile Thr Leu Asp Glu Leu Pro 
465                 470                 475                 480 


Ala Asp Cys Thr Pro Ile Leu Met Ile Arg Gln Arg Met Val Asn Gln 
                485                 490                 495     


Gln Gly His His His His His His 
            500                 


<210>  7
<211>  1014
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct DNA sequence of truncated GH26 mannanase missing the 
       CBM35 domain and a 6xHis tagged added directly on the C-terminal 
       of the protein


<220>
<221>  CDS
<222>  (1)..(1011)

<400>  7
atg atc agg act ggc aca ttg aac aat ccc gaa gcg aca gct gag gcc         48
Met Ile Arg Thr Gly Thr Leu Asn Asn Pro Glu Ala Thr Ala Glu Ala           
1               5                   10                  15                

aga gcg cta atg aac tac ttg ctc agc cag tat gga caa aaa att atc         96
Arg Ala Leu Met Asn Tyr Leu Leu Ser Gln Tyr Gly Gln Lys Ile Ile           
            20                  25                  30                    

tct gga cag cag acg ctt gaa gat gtg gag tgg atc aag cag cag aca        144
Ser Gly Gln Gln Thr Leu Glu Asp Val Glu Trp Ile Lys Gln Gln Thr           
        35                  40                  45                        

ggc aaa tat cca gcg att ttc tct aca gac ttg atg gat tac tcc cct        192
Gly Lys Tyr Pro Ala Ile Phe Ser Thr Asp Leu Met Asp Tyr Ser Pro           
    50                  55                  60                            

tcc cgc gtg gat cat gga gcc tcc tcc act gag gtc gag aag atg atc        240
Ser Arg Val Asp His Gly Ala Ser Ser Thr Glu Val Glu Lys Met Ile           
65                  70                  75                  80            

gaa tgg tac aaa cgc ggt ggt att gtg tct tta tgc tgg cac tgg aat        288
Glu Trp Tyr Lys Arg Gly Gly Ile Val Ser Leu Cys Trp His Trp Asn           
                85                  90                  95                

gcc ccg aag gga atc ggc ggc aat gag cct ggc aac gag tgg tgg cga        336
Ala Pro Lys Gly Ile Gly Gly Asn Glu Pro Gly Asn Glu Trp Trp Arg           
            100                 105                 110                   

ggc ttc tac act gaa ttt aca acc ttt gat gtg gaa tat gct ctt aat        384
Gly Phe Tyr Thr Glu Phe Thr Thr Phe Asp Val Glu Tyr Ala Leu Asn           
        115                 120                 125                       

cat ccg gat tct gag gac tac cag ctc ctg atc cgg gac att gac gcc        432
His Pro Asp Ser Glu Asp Tyr Gln Leu Leu Ile Arg Asp Ile Asp Ala           
    130                 135                 140                           

atc gca gtt cag ttg aag cga ttg cag gag gcg aac gtg cct gtg tta        480
Ile Ala Val Gln Leu Lys Arg Leu Gln Glu Ala Asn Val Pro Val Leu           
145                 150                 155                 160           

tgg cga ccc ctg cac gag gca gag ggc acc tgg ttt tgg tgg gga gca        528
Trp Arg Pro Leu His Glu Ala Glu Gly Thr Trp Phe Trp Trp Gly Ala           
                165                 170                 175               

aaa ggg ccc gag ccg gcg aaa cag ctc tat cgt tta atg tat gat cgg        576
Lys Gly Pro Glu Pro Ala Lys Gln Leu Tyr Arg Leu Met Tyr Asp Arg           
            180                 185                 190                   

tta acc aat gat cat aag ctg aac aat ctg att tgg gtg tgg aac tcc        624
Leu Thr Asn Asp His Lys Leu Asn Asn Leu Ile Trp Val Trp Asn Ser           
        195                 200                 205                       

gag aaa aag gat tgg tat ccg gga gat gat gtc gta gat atg gta agc        672
Glu Lys Lys Asp Trp Tyr Pro Gly Asp Asp Val Val Asp Met Val Ser           
    210                 215                 220                           

gtt gat atc tac aac cct gca ggc gac tat aat ccg agc atc gca aaa        720
Val Asp Ile Tyr Asn Pro Ala Gly Asp Tyr Asn Pro Ser Ile Ala Lys           
225                 230                 235                 240           

tat gaa gcg ctt gta tct ttg gcg gac aac aag aag atg gct gca cta        768
Tyr Glu Ala Leu Val Ser Leu Ala Asp Asn Lys Lys Met Ala Ala Leu           
                245                 250                 255               

gcg gag aat ggg cct att ccg gat ccg gat gct ctt cag gag tac ggc        816
Ala Glu Asn Gly Pro Ile Pro Asp Pro Asp Ala Leu Gln Glu Tyr Gly           
            260                 265                 270                   

gcc gac tgg agc ttc ttt agt acc tgg acc ggc gac tac atc agg gat        864
Ala Asp Trp Ser Phe Phe Ser Thr Trp Thr Gly Asp Tyr Ile Arg Asp           
        275                 280                 285                       

ggc aag aca aat acg ata gaa cat ttg aag aag gta tat caa cac gat        912
Gly Lys Thr Asn Thr Ile Glu His Leu Lys Lys Val Tyr Gln His Asp           
    290                 295                 300                           

tac gtc att act ctc gac gaa ctc ccg gca gac tgt act cca atc ttg        960
Tyr Val Ile Thr Leu Asp Glu Leu Pro Ala Asp Cys Thr Pro Ile Leu           
305                 310                 315                 320           

atg ata agg caa aga atg gtg aat cag cag gga cat cat cac cat cac       1008
Met Ile Arg Gln Arg Met Val Asn Gln Gln Gly His His His His His           
                325                 330                 335               

cac taa                                                               1014
His                                                                       
                                                                          


<210>  8
<211>  337
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  8

Met Ile Arg Thr Gly Thr Leu Asn Asn Pro Glu Ala Thr Ala Glu Ala 
1               5                   10                  15      


Arg Ala Leu Met Asn Tyr Leu Leu Ser Gln Tyr Gly Gln Lys Ile Ile 
            20                  25                  30          


Ser Gly Gln Gln Thr Leu Glu Asp Val Glu Trp Ile Lys Gln Gln Thr 
        35                  40                  45              


Gly Lys Tyr Pro Ala Ile Phe Ser Thr Asp Leu Met Asp Tyr Ser Pro 
    50                  55                  60                  


Ser Arg Val Asp His Gly Ala Ser Ser Thr Glu Val Glu Lys Met Ile 
65                  70                  75                  80  


Glu Trp Tyr Lys Arg Gly Gly Ile Val Ser Leu Cys Trp His Trp Asn 
                85                  90                  95      


Ala Pro Lys Gly Ile Gly Gly Asn Glu Pro Gly Asn Glu Trp Trp Arg 
            100                 105                 110         


Gly Phe Tyr Thr Glu Phe Thr Thr Phe Asp Val Glu Tyr Ala Leu Asn 
        115                 120                 125             


His Pro Asp Ser Glu Asp Tyr Gln Leu Leu Ile Arg Asp Ile Asp Ala 
    130                 135                 140                 


Ile Ala Val Gln Leu Lys Arg Leu Gln Glu Ala Asn Val Pro Val Leu 
145                 150                 155                 160 


Trp Arg Pro Leu His Glu Ala Glu Gly Thr Trp Phe Trp Trp Gly Ala 
                165                 170                 175     


Lys Gly Pro Glu Pro Ala Lys Gln Leu Tyr Arg Leu Met Tyr Asp Arg 
            180                 185                 190         


Leu Thr Asn Asp His Lys Leu Asn Asn Leu Ile Trp Val Trp Asn Ser 
        195                 200                 205             


Glu Lys Lys Asp Trp Tyr Pro Gly Asp Asp Val Val Asp Met Val Ser 
    210                 215                 220                 


Val Asp Ile Tyr Asn Pro Ala Gly Asp Tyr Asn Pro Ser Ile Ala Lys 
225                 230                 235                 240 


Tyr Glu Ala Leu Val Ser Leu Ala Asp Asn Lys Lys Met Ala Ala Leu 
                245                 250                 255     


Ala Glu Asn Gly Pro Ile Pro Asp Pro Asp Ala Leu Gln Glu Tyr Gly 
            260                 265                 270         


Ala Asp Trp Ser Phe Phe Ser Thr Trp Thr Gly Asp Tyr Ile Arg Asp 
        275                 280                 285             


Gly Lys Thr Asn Thr Ile Glu His Leu Lys Lys Val Tyr Gln His Asp 
    290                 295                 300                 


Tyr Val Ile Thr Leu Asp Glu Leu Pro Ala Asp Cys Thr Pro Ile Leu 
305                 310                 315                 320 


Met Ile Arg Gln Arg Met Val Asn Gln Gln Gly His His His His His 
                325                 330                 335     


His 
    


<210>  9
<211>  1704
<212>  DNA
<213>  Paenibacillus ihumii


<220>
<221>  CDS
<222>  (1)..(1704)

<220>
<221>  sig_peptide
<222>  (1)..(123)

<220>
<221>  mat_peptide
<222>  (124)..(1704)

<400>  9
atg aaa gca atg tct aac ctt ctt gta gct gcg gag cag cgc aaa cgt         48
Met Lys Ala Met Ser Asn Leu Leu Val Ala Ala Glu Gln Arg Lys Arg           
    -40                 -35                 -30                           

cgc ttc agc atc ttt ctt ttc tgt ttc gta atc ctt gct gga act tac         96
Arg Phe Ser Ile Phe Leu Phe Cys Phe Val Ile Leu Ala Gly Thr Tyr           
-25                 -20                 -15                 -10           

gga tca gcg gca cct atc gcg gac gct agc tct gca ctt cct gac acg        144
Gly Ser Ala Ala Pro Ile Ala Asp Ala Ser Ser Ala Leu Pro Asp Thr           
                -5              -1  1               5                     

gtt gta aaa gag gct cct tct gct tct cct act aac ggc atc aca gta        192
Val Val Lys Glu Ala Pro Ser Ala Ser Pro Thr Asn Gly Ile Thr Val           
        10                  15                  20                        

aca ttt gct gac gct gtt ctt act gga tac ggc atc gag aaa cgc ggt        240
Thr Phe Ala Asp Ala Val Leu Thr Gly Tyr Gly Ile Glu Lys Arg Gly           
    25                  30                  35                            

tct gta aag gag aac gag gac acg ctt tac gac ggc aaa ggt tac atc        288
Ser Val Lys Glu Asn Glu Asp Thr Leu Tyr Asp Gly Lys Gly Tyr Ile           
40                  45                  50                  55            

tca tac ttc ttc gac gag gac gct aac gct gct gag cct gtt ggc tct        336
Ser Tyr Phe Phe Asp Glu Asp Ala Asn Ala Ala Glu Pro Val Gly Ser           
                60                  65                  70                

gct aca ttc act gtt gac gta gcg gaa gct ggc ctt tac aaa ctt tca        384
Ala Thr Phe Thr Val Asp Val Ala Glu Ala Gly Leu Tyr Lys Leu Ser           
            75                  80                  85                    

ctt ggc tac tat ctt cct gag ggc tat ggc gac aaa gtt act tct atc        432
Leu Gly Tyr Tyr Leu Pro Glu Gly Tyr Gly Asp Lys Val Thr Ser Ile           
        90                  95                  100                       

gag atc aat ggc gca ggt aca gga gag ctt aca ctt gac gag cct gct        480
Glu Ile Asn Gly Ala Gly Thr Gly Glu Leu Thr Leu Asp Glu Pro Ala           
    105                 110                 115                           

gcc ggt act gtt cgc gct gag aaa atg gtt tca aaa gtt ctt ctt aac        528
Ala Gly Thr Val Arg Ala Glu Lys Met Val Ser Lys Val Leu Leu Asn           
120                 125                 130                 135           

gca ggc agc aac aca atc aaa atc atg cgt ggt tgg ggc tat tac ggc        576
Ala Gly Ser Asn Thr Ile Lys Ile Met Arg Gly Trp Gly Tyr Tyr Gly           
                140                 145                 150               

atc gag cac atc aaa ctt gag cct gct ggc gca gca tca tct tca aac        624
Ile Glu His Ile Lys Leu Glu Pro Ala Gly Ala Ala Ser Ser Ser Asn           
            155                 160                 165                   

aag ctt gct gct gag gac ggt cct atg aca ggc gca ctt aac aac cct        672
Lys Leu Ala Ala Glu Asp Gly Pro Met Thr Gly Ala Leu Asn Asn Pro           
        170                 175                 180                       

gag gct aca cct gag gct cgc gca ctt atg gac tat ctt ctt tct cag        720
Glu Ala Thr Pro Glu Ala Arg Ala Leu Met Asp Tyr Leu Leu Ser Gln           
    185                 190                 195                           

tac ggc cag aaa atc atc tct ggt caa caa act atc gag gac atc gag        768
Tyr Gly Gln Lys Ile Ile Ser Gly Gln Gln Thr Ile Glu Asp Ile Glu           
200                 205                 210                 215           

tgg atc aaa caa caa aca ggc aag tat cct gct atc ttc agc acg gac        816
Trp Ile Lys Gln Gln Thr Gly Lys Tyr Pro Ala Ile Phe Ser Thr Asp           
                220                 225                 230               

ctt atg gac tat tca cct tct cgc atc gag aat ggc gca tct tct act        864
Leu Met Asp Tyr Ser Pro Ser Arg Ile Glu Asn Gly Ala Ser Ser Thr           
            235                 240                 245                   

gag gtt gag aaa atg atc gag tgg tac aaa cgc ggt ggc atc gtt tca        912
Glu Val Glu Lys Met Ile Glu Trp Tyr Lys Arg Gly Gly Ile Val Ser           
        250                 255                 260                       

ctt tgt tgg cac tgg aac gct cct aag gga atc ggt ggc aac gag cct        960
Leu Cys Trp His Trp Asn Ala Pro Lys Gly Ile Gly Gly Asn Glu Pro           
    265                 270                 275                           

ggt cat gag tgg tgg cgt ggc ttc tat acg gag ttt acg acg ttc gac       1008
Gly His Glu Trp Trp Arg Gly Phe Tyr Thr Glu Phe Thr Thr Phe Asp           
280                 285                 290                 295           

gtt gag ttt gca ctt aac cat tta gac agc gag gac tac caa ctt ctt       1056
Val Glu Phe Ala Leu Asn His Leu Asp Ser Glu Asp Tyr Gln Leu Leu           
                300                 305                 310               

atc cgc gac atc gac gcc atc gct gtt cag ctt aaa cgc ctt caa gac       1104
Ile Arg Asp Ile Asp Ala Ile Ala Val Gln Leu Lys Arg Leu Gln Asp           
            315                 320                 325                   

gct aac gta cct gtt ctt tgg cgt cct ctt cac gag gca gag gga ggc       1152
Ala Asn Val Pro Val Leu Trp Arg Pro Leu His Glu Ala Glu Gly Gly           
        330                 335                 340                       

tgg ttc tgg tgg gga gcg aaa ggt cct gag cct gca aaa caa ctt tat       1200
Trp Phe Trp Trp Gly Ala Lys Gly Pro Glu Pro Ala Lys Gln Leu Tyr           
    345                 350                 355                           

cgc ctt atg tat gac cgc ctt aca cac gac cat aac ctt aac aac ctt       1248
Arg Leu Met Tyr Asp Arg Leu Thr His Asp His Asn Leu Asn Asn Leu           
360                 365                 370                 375           

atc tgg atc tgg aac agc gag aag aaa gac tgg tac cct ggt gac gac       1296
Ile Trp Ile Trp Asn Ser Glu Lys Lys Asp Trp Tyr Pro Gly Asp Asp           
                380                 385                 390               

gtt gta gac atc gta tct gta gac atc tac aac cct gct gag gac tac       1344
Val Val Asp Ile Val Ser Val Asp Ile Tyr Asn Pro Ala Glu Asp Tyr           
            395                 400                 405                   

aac cct tct atc gcg aaa tac gag ggc ctt gtt tca ctt gta aac ggc       1392
Asn Pro Ser Ile Ala Lys Tyr Glu Gly Leu Val Ser Leu Val Asn Gly           
        410                 415                 420                       

aag aaa atg gca gct ctt gca gag aat ggc cct atc cct gac cct gac       1440
Lys Lys Met Ala Ala Leu Ala Glu Asn Gly Pro Ile Pro Asp Pro Asp           
    425                 430                 435                           

gct ctt caa gca tat ggc gct gag tgg agc ttc ttt tca act tgg aca       1488
Ala Leu Gln Ala Tyr Gly Ala Glu Trp Ser Phe Phe Ser Thr Trp Thr           
440                 445                 450                 455           

ggc gac tat atc cgc gac gga aaa act aac act atg gag cat ctt aag       1536
Gly Asp Tyr Ile Arg Asp Gly Lys Thr Asn Thr Met Glu His Leu Lys           
                460                 465                 470               

aag gta tac cat cat gac tac gta atc act ctt gac gag ctt cct gcc       1584
Lys Val Tyr His His Asp Tyr Val Ile Thr Leu Asp Glu Leu Pro Ala           
            475                 480                 485                   

gac ctt tac gct aac cca gag ttt gag gct gag aac ggt gag tca gcg       1632
Asp Leu Tyr Ala Asn Pro Glu Phe Glu Ala Glu Asn Gly Glu Ser Ala           
        490                 495                 500                       

gga atg act cgc gct aac ggt cag gag tct cac tct aaa ggt ggc tac       1680
Gly Met Thr Arg Ala Asn Gly Gln Glu Ser His Ser Lys Gly Gly Tyr           
    505                 510                 515                           

act act ggc atg gag cct aag aac                                       1704
Thr Thr Gly Met Glu Pro Lys Asn                                           
520                 525                                                   


<210>  10
<211>  568
<212>  PRT
<213>  Paenibacillus ihumii

<400>  10

Met Lys Ala Met Ser Asn Leu Leu Val Ala Ala Glu Gln Arg Lys Arg 
    -40                 -35                 -30                 


Arg Phe Ser Ile Phe Leu Phe Cys Phe Val Ile Leu Ala Gly Thr Tyr 
-25                 -20                 -15                 -10 


Gly Ser Ala Ala Pro Ile Ala Asp Ala Ser Ser Ala Leu Pro Asp Thr 
                -5              -1  1               5           


Val Val Lys Glu Ala Pro Ser Ala Ser Pro Thr Asn Gly Ile Thr Val 
        10                  15                  20              


Thr Phe Ala Asp Ala Val Leu Thr Gly Tyr Gly Ile Glu Lys Arg Gly 
    25                  30                  35                  


Ser Val Lys Glu Asn Glu Asp Thr Leu Tyr Asp Gly Lys Gly Tyr Ile 
40                  45                  50                  55  


Ser Tyr Phe Phe Asp Glu Asp Ala Asn Ala Ala Glu Pro Val Gly Ser 
                60                  65                  70      


Ala Thr Phe Thr Val Asp Val Ala Glu Ala Gly Leu Tyr Lys Leu Ser 
            75                  80                  85          


Leu Gly Tyr Tyr Leu Pro Glu Gly Tyr Gly Asp Lys Val Thr Ser Ile 
        90                  95                  100             


Glu Ile Asn Gly Ala Gly Thr Gly Glu Leu Thr Leu Asp Glu Pro Ala 
    105                 110                 115                 


Ala Gly Thr Val Arg Ala Glu Lys Met Val Ser Lys Val Leu Leu Asn 
120                 125                 130                 135 


Ala Gly Ser Asn Thr Ile Lys Ile Met Arg Gly Trp Gly Tyr Tyr Gly 
                140                 145                 150     


Ile Glu His Ile Lys Leu Glu Pro Ala Gly Ala Ala Ser Ser Ser Asn 
            155                 160                 165         


Lys Leu Ala Ala Glu Asp Gly Pro Met Thr Gly Ala Leu Asn Asn Pro 
        170                 175                 180             


Glu Ala Thr Pro Glu Ala Arg Ala Leu Met Asp Tyr Leu Leu Ser Gln 
    185                 190                 195                 


Tyr Gly Gln Lys Ile Ile Ser Gly Gln Gln Thr Ile Glu Asp Ile Glu 
200                 205                 210                 215 


Trp Ile Lys Gln Gln Thr Gly Lys Tyr Pro Ala Ile Phe Ser Thr Asp 
                220                 225                 230     


Leu Met Asp Tyr Ser Pro Ser Arg Ile Glu Asn Gly Ala Ser Ser Thr 
            235                 240                 245         


Glu Val Glu Lys Met Ile Glu Trp Tyr Lys Arg Gly Gly Ile Val Ser 
        250                 255                 260             


Leu Cys Trp His Trp Asn Ala Pro Lys Gly Ile Gly Gly Asn Glu Pro 
    265                 270                 275                 


Gly His Glu Trp Trp Arg Gly Phe Tyr Thr Glu Phe Thr Thr Phe Asp 
280                 285                 290                 295 


Val Glu Phe Ala Leu Asn His Leu Asp Ser Glu Asp Tyr Gln Leu Leu 
                300                 305                 310     


Ile Arg Asp Ile Asp Ala Ile Ala Val Gln Leu Lys Arg Leu Gln Asp 
            315                 320                 325         


Ala Asn Val Pro Val Leu Trp Arg Pro Leu His Glu Ala Glu Gly Gly 
        330                 335                 340             


Trp Phe Trp Trp Gly Ala Lys Gly Pro Glu Pro Ala Lys Gln Leu Tyr 
    345                 350                 355                 


Arg Leu Met Tyr Asp Arg Leu Thr His Asp His Asn Leu Asn Asn Leu 
360                 365                 370                 375 


Ile Trp Ile Trp Asn Ser Glu Lys Lys Asp Trp Tyr Pro Gly Asp Asp 
                380                 385                 390     


Val Val Asp Ile Val Ser Val Asp Ile Tyr Asn Pro Ala Glu Asp Tyr 
            395                 400                 405         


Asn Pro Ser Ile Ala Lys Tyr Glu Gly Leu Val Ser Leu Val Asn Gly 
        410                 415                 420             


Lys Lys Met Ala Ala Leu Ala Glu Asn Gly Pro Ile Pro Asp Pro Asp 
    425                 430                 435                 


Ala Leu Gln Ala Tyr Gly Ala Glu Trp Ser Phe Phe Ser Thr Trp Thr 
440                 445                 450                 455 


Gly Asp Tyr Ile Arg Asp Gly Lys Thr Asn Thr Met Glu His Leu Lys 
                460                 465                 470     


Lys Val Tyr His His Asp Tyr Val Ile Thr Leu Asp Glu Leu Pro Ala 
            475                 480                 485         


Asp Leu Tyr Ala Asn Pro Glu Phe Glu Ala Glu Asn Gly Glu Ser Ala 
        490                 495                 500             


Gly Met Thr Arg Ala Asn Gly Gln Glu Ser His Ser Lys Gly Gly Tyr 
    505                 510                 515                 


Thr Thr Gly Met Glu Pro Lys Asn 
520                 525         


<210>  11
<211>  527
<212>  PRT
<213>  Paenibacillus ihumii


<220>
<221>  mat_peptide
<222>  (1)..(527)

<400>  11

Ser Ser Ala Leu Pro Asp Thr Val Val Lys Glu Ala Pro Ser Ala Ser 
1               5                   10                  15      


Pro Thr Asn Gly Ile Thr Val Thr Phe Ala Asp Ala Val Leu Thr Gly 
            20                  25                  30          


Tyr Gly Ile Glu Lys Arg Gly Ser Val Lys Glu Asn Glu Asp Thr Leu 
        35                  40                  45              


Tyr Asp Gly Lys Gly Tyr Ile Ser Tyr Phe Phe Asp Glu Asp Ala Asn 
    50                  55                  60                  


Ala Ala Glu Pro Val Gly Ser Ala Thr Phe Thr Val Asp Val Ala Glu 
65                  70                  75                  80  


Ala Gly Leu Tyr Lys Leu Ser Leu Gly Tyr Tyr Leu Pro Glu Gly Tyr 
                85                  90                  95      


Gly Asp Lys Val Thr Ser Ile Glu Ile Asn Gly Ala Gly Thr Gly Glu 
            100                 105                 110         


Leu Thr Leu Asp Glu Pro Ala Ala Gly Thr Val Arg Ala Glu Lys Met 
        115                 120                 125             


Val Ser Lys Val Leu Leu Asn Ala Gly Ser Asn Thr Ile Lys Ile Met 
    130                 135                 140                 


Arg Gly Trp Gly Tyr Tyr Gly Ile Glu His Ile Lys Leu Glu Pro Ala 
145                 150                 155                 160 


Gly Ala Ala Ser Ser Ser Asn Lys Leu Ala Ala Glu Asp Gly Pro Met 
                165                 170                 175     


Thr Gly Ala Leu Asn Asn Pro Glu Ala Thr Pro Glu Ala Arg Ala Leu 
            180                 185                 190         


Met Asp Tyr Leu Leu Ser Gln Tyr Gly Gln Lys Ile Ile Ser Gly Gln 
        195                 200                 205             


Gln Thr Ile Glu Asp Ile Glu Trp Ile Lys Gln Gln Thr Gly Lys Tyr 
    210                 215                 220                 


Pro Ala Ile Phe Ser Thr Asp Leu Met Asp Tyr Ser Pro Ser Arg Ile 
225                 230                 235                 240 


Glu Asn Gly Ala Ser Ser Thr Glu Val Glu Lys Met Ile Glu Trp Tyr 
                245                 250                 255     


Lys Arg Gly Gly Ile Val Ser Leu Cys Trp His Trp Asn Ala Pro Lys 
            260                 265                 270         


Gly Ile Gly Gly Asn Glu Pro Gly His Glu Trp Trp Arg Gly Phe Tyr 
        275                 280                 285             


Thr Glu Phe Thr Thr Phe Asp Val Glu Phe Ala Leu Asn His Leu Asp 
    290                 295                 300                 


Ser Glu Asp Tyr Gln Leu Leu Ile Arg Asp Ile Asp Ala Ile Ala Val 
305                 310                 315                 320 


Gln Leu Lys Arg Leu Gln Asp Ala Asn Val Pro Val Leu Trp Arg Pro 
                325                 330                 335     


Leu His Glu Ala Glu Gly Gly Trp Phe Trp Trp Gly Ala Lys Gly Pro 
            340                 345                 350         


Glu Pro Ala Lys Gln Leu Tyr Arg Leu Met Tyr Asp Arg Leu Thr His 
        355                 360                 365             


Asp His Asn Leu Asn Asn Leu Ile Trp Ile Trp Asn Ser Glu Lys Lys 
    370                 375                 380                 


Asp Trp Tyr Pro Gly Asp Asp Val Val Asp Ile Val Ser Val Asp Ile 
385                 390                 395                 400 


Tyr Asn Pro Ala Glu Asp Tyr Asn Pro Ser Ile Ala Lys Tyr Glu Gly 
                405                 410                 415     


Leu Val Ser Leu Val Asn Gly Lys Lys Met Ala Ala Leu Ala Glu Asn 
            420                 425                 430         


Gly Pro Ile Pro Asp Pro Asp Ala Leu Gln Ala Tyr Gly Ala Glu Trp 
        435                 440                 445             


Ser Phe Phe Ser Thr Trp Thr Gly Asp Tyr Ile Arg Asp Gly Lys Thr 
    450                 455                 460                 


Asn Thr Met Glu His Leu Lys Lys Val Tyr His His Asp Tyr Val Ile 
465                 470                 475                 480 


Thr Leu Asp Glu Leu Pro Ala Asp Leu Tyr Ala Asn Pro Glu Phe Glu 
                485                 490                 495     


Ala Glu Asn Gly Glu Ser Ala Gly Met Thr Arg Ala Asn Gly Gln Glu 
            500                 505                 510         


Ser His Ser Lys Gly Gly Tyr Thr Thr Gly Met Glu Pro Lys Asn 
        515                 520                 525         


<210>  12
<211>  1689
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Construct DNA sequence of GH26 mannanase from P.ihumii comprising
       CBM#5 domain where native secretion signal was replaced with 
       Bacillus licheniformis secretion signal and a HPHPHPHP tag was 
       added directly to the C-terminal


<220>
<221>  CDS
<222>  (1)..(1686)

<220>
<221>  sig_peptide
<222>  (1)..(81)

<220>
<221>  mat_peptide
<222>  (82)..(1686)

<400>  12
atg aag aaa ccg ttg ggg aaa att gtc gca agc acc gca cta ctc att         48
Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile           
        -25                 -20                 -15                       

tct gtt gct ttt agt tca tcg ata gca tca gca agc tct gca ctt cct         96
Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Ser Ser Ala Leu Pro           
    -10                 -5              -1  1               5             

gac acg gtt gta aaa gag gct cct tct gct tct cct act aac ggc atc        144
Asp Thr Val Val Lys Glu Ala Pro Ser Ala Ser Pro Thr Asn Gly Ile           
                10                  15                  20                

aca gta aca ttt gct gac gct gtt ctt act gga tac ggc atc gag aaa        192
Thr Val Thr Phe Ala Asp Ala Val Leu Thr Gly Tyr Gly Ile Glu Lys           
            25                  30                  35                    

cgc ggt tct gta aag gag aac gag gac acg ctt tac gac ggc aaa ggt        240
Arg Gly Ser Val Lys Glu Asn Glu Asp Thr Leu Tyr Asp Gly Lys Gly           
        40                  45                  50                        

tac atc tca tac ttc ttc gac gag gac gct aac gct gct gag cct gtt        288
Tyr Ile Ser Tyr Phe Phe Asp Glu Asp Ala Asn Ala Ala Glu Pro Val           
    55                  60                  65                            

ggc tct gct aca ttc act gtt gac gta gcg gaa gct ggc ctt tac aaa        336
Gly Ser Ala Thr Phe Thr Val Asp Val Ala Glu Ala Gly Leu Tyr Lys           
70                  75                  80                  85            

ctt tca ctt ggc tac tat ctt cct gag ggc tat ggc gac aaa gtt act        384
Leu Ser Leu Gly Tyr Tyr Leu Pro Glu Gly Tyr Gly Asp Lys Val Thr           
                90                  95                  100               

tct atc gag atc aat ggc gca ggt aca gga gag ctt aca ctt gac gag        432
Ser Ile Glu Ile Asn Gly Ala Gly Thr Gly Glu Leu Thr Leu Asp Glu           
            105                 110                 115                   

cct gct gcc ggt act gtt cgc gct gag aaa atg gtt tca aaa gtt ctt        480
Pro Ala Ala Gly Thr Val Arg Ala Glu Lys Met Val Ser Lys Val Leu           
        120                 125                 130                       

ctt aac gca ggc agc aac aca atc aaa atc atg cgt ggt tgg ggc tat        528
Leu Asn Ala Gly Ser Asn Thr Ile Lys Ile Met Arg Gly Trp Gly Tyr           
    135                 140                 145                           

tac ggc atc gag cac atc aaa ctt gag cct gct ggc gca gca tca tct        576
Tyr Gly Ile Glu His Ile Lys Leu Glu Pro Ala Gly Ala Ala Ser Ser           
150                 155                 160                 165           

tca aac aag ctt gct gct gag gac ggt cct atg aca ggc gca ctt aac        624
Ser Asn Lys Leu Ala Ala Glu Asp Gly Pro Met Thr Gly Ala Leu Asn           
                170                 175                 180               

aac cct gag gct aca cct gag gct cgc gca ctt atg gac tat ctt ctt        672
Asn Pro Glu Ala Thr Pro Glu Ala Arg Ala Leu Met Asp Tyr Leu Leu           
            185                 190                 195                   

tct cag tac ggc cag aaa atc atc tct ggt caa caa act atc gag gac        720
Ser Gln Tyr Gly Gln Lys Ile Ile Ser Gly Gln Gln Thr Ile Glu Asp           
        200                 205                 210                       

atc gag tgg atc aaa caa caa aca ggc aag tat cct gct atc ttc agc        768
Ile Glu Trp Ile Lys Gln Gln Thr Gly Lys Tyr Pro Ala Ile Phe Ser           
    215                 220                 225                           

acg gac ctt atg gac tat tca cct tct cgc atc gag aat ggc gca tct        816
Thr Asp Leu Met Asp Tyr Ser Pro Ser Arg Ile Glu Asn Gly Ala Ser           
230                 235                 240                 245           

tct act gag gtt gag aaa atg atc gag tgg tac aaa cgc ggt ggc atc        864
Ser Thr Glu Val Glu Lys Met Ile Glu Trp Tyr Lys Arg Gly Gly Ile           
                250                 255                 260               

gtt tca ctt tgt tgg cac tgg aac gct cct aag gga atc ggt ggc aac        912
Val Ser Leu Cys Trp His Trp Asn Ala Pro Lys Gly Ile Gly Gly Asn           
            265                 270                 275                   

gag cct ggt cat gag tgg tgg cgt ggc ttc tat acg gag ttt acg acg        960
Glu Pro Gly His Glu Trp Trp Arg Gly Phe Tyr Thr Glu Phe Thr Thr           
        280                 285                 290                       

ttc gac gtt gag ttt gca ctt aac cat tta gac agc gag gac tac caa       1008
Phe Asp Val Glu Phe Ala Leu Asn His Leu Asp Ser Glu Asp Tyr Gln           
    295                 300                 305                           

ctt ctt atc cgc gac atc gac gcc atc gct gtt cag ctt aaa cgc ctt       1056
Leu Leu Ile Arg Asp Ile Asp Ala Ile Ala Val Gln Leu Lys Arg Leu           
310                 315                 320                 325           

caa gac gct aac gta cct gtt ctt tgg cgt cct ctt cac gag gca gag       1104
Gln Asp Ala Asn Val Pro Val Leu Trp Arg Pro Leu His Glu Ala Glu           
                330                 335                 340               

gga ggc tgg ttc tgg tgg gga gcg aaa ggt cct gag cct gca aaa caa       1152
Gly Gly Trp Phe Trp Trp Gly Ala Lys Gly Pro Glu Pro Ala Lys Gln           
            345                 350                 355                   

ctt tat cgc ctt atg tat gac cgc ctt aca cac gac cat aac ctt aac       1200
Leu Tyr Arg Leu Met Tyr Asp Arg Leu Thr His Asp His Asn Leu Asn           
        360                 365                 370                       

aac ctt atc tgg atc tgg aac agc gag aag aaa gac tgg tac cct ggt       1248
Asn Leu Ile Trp Ile Trp Asn Ser Glu Lys Lys Asp Trp Tyr Pro Gly           
    375                 380                 385                           

gac gac gtt gta gac atc gta tct gta gac atc tac aac cct gct gag       1296
Asp Asp Val Val Asp Ile Val Ser Val Asp Ile Tyr Asn Pro Ala Glu           
390                 395                 400                 405           

gac tac aac cct tct atc gcg aaa tac gag ggc ctt gtt tca ctt gta       1344
Asp Tyr Asn Pro Ser Ile Ala Lys Tyr Glu Gly Leu Val Ser Leu Val           
                410                 415                 420               

aac ggc aag aaa atg gca gct ctt gca gag aat ggc cct atc cct gac       1392
Asn Gly Lys Lys Met Ala Ala Leu Ala Glu Asn Gly Pro Ile Pro Asp           
            425                 430                 435                   

cct gac gct ctt caa gca tat ggc gct gag tgg agc ttc ttt tca act       1440
Pro Asp Ala Leu Gln Ala Tyr Gly Ala Glu Trp Ser Phe Phe Ser Thr           
        440                 445                 450                       

tgg aca ggc gac tat atc cgc gac gga aaa act aac act atg gag cat       1488
Trp Thr Gly Asp Tyr Ile Arg Asp Gly Lys Thr Asn Thr Met Glu His           
    455                 460                 465                           

ctt aag aag gta tac cat cat gac tac gta atc act ctt gac gag ctt       1536
Leu Lys Lys Val Tyr His His Asp Tyr Val Ile Thr Leu Asp Glu Leu           
470                 475                 480                 485           

cct gcc gac ctt tac gct aac cca gag ttt gag gct gag aac ggt gag       1584
Pro Ala Asp Leu Tyr Ala Asn Pro Glu Phe Glu Ala Glu Asn Gly Glu           
                490                 495                 500               

tca gcg gga atg act cgc gct aac ggt cag gag tct cac tct aaa ggt       1632
Ser Ala Gly Met Thr Arg Ala Asn Gly Gln Glu Ser His Ser Lys Gly           
            505                 510                 515                   

ggc tac act act ggc atg gag cct aag aac cat ccg cac cct cat cca       1680
Gly Tyr Thr Thr Gly Met Glu Pro Lys Asn His Pro His Pro His Pro           
        520                 525                 530                       

cac ccg taa                                                           1689
His Pro                                                                   
    535                                                                   


<210>  13
<211>  562
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  13

Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile 
        -25                 -20                 -15             


Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala Ser Ser Ala Leu Pro 
    -10                 -5              -1  1               5   


Asp Thr Val Val Lys Glu Ala Pro Ser Ala Ser Pro Thr Asn Gly Ile 
                10                  15                  20      


Thr Val Thr Phe Ala Asp Ala Val Leu Thr Gly Tyr Gly Ile Glu Lys 
            25                  30                  35          


Arg Gly Ser Val Lys Glu Asn Glu Asp Thr Leu Tyr Asp Gly Lys Gly 
        40                  45                  50              


Tyr Ile Ser Tyr Phe Phe Asp Glu Asp Ala Asn Ala Ala Glu Pro Val 
    55                  60                  65                  


Gly Ser Ala Thr Phe Thr Val Asp Val Ala Glu Ala Gly Leu Tyr Lys 
70                  75                  80                  85  


Leu Ser Leu Gly Tyr Tyr Leu Pro Glu Gly Tyr Gly Asp Lys Val Thr 
                90                  95                  100     


Ser Ile Glu Ile Asn Gly Ala Gly Thr Gly Glu Leu Thr Leu Asp Glu 
            105                 110                 115         


Pro Ala Ala Gly Thr Val Arg Ala Glu Lys Met Val Ser Lys Val Leu 
        120                 125                 130             


Leu Asn Ala Gly Ser Asn Thr Ile Lys Ile Met Arg Gly Trp Gly Tyr 
    135                 140                 145                 


Tyr Gly Ile Glu His Ile Lys Leu Glu Pro Ala Gly Ala Ala Ser Ser 
150                 155                 160                 165 


Ser Asn Lys Leu Ala Ala Glu Asp Gly Pro Met Thr Gly Ala Leu Asn 
                170                 175                 180     


Asn Pro Glu Ala Thr Pro Glu Ala Arg Ala Leu Met Asp Tyr Leu Leu 
            185                 190                 195         


Ser Gln Tyr Gly Gln Lys Ile Ile Ser Gly Gln Gln Thr Ile Glu Asp 
        200                 205                 210             


Ile Glu Trp Ile Lys Gln Gln Thr Gly Lys Tyr Pro Ala Ile Phe Ser 
    215                 220                 225                 


Thr Asp Leu Met Asp Tyr Ser Pro Ser Arg Ile Glu Asn Gly Ala Ser 
230                 235                 240                 245 


Ser Thr Glu Val Glu Lys Met Ile Glu Trp Tyr Lys Arg Gly Gly Ile 
                250                 255                 260     


Val Ser Leu Cys Trp His Trp Asn Ala Pro Lys Gly Ile Gly Gly Asn 
            265                 270                 275         


Glu Pro Gly His Glu Trp Trp Arg Gly Phe Tyr Thr Glu Phe Thr Thr 
        280                 285                 290             


Phe Asp Val Glu Phe Ala Leu Asn His Leu Asp Ser Glu Asp Tyr Gln 
    295                 300                 305                 


Leu Leu Ile Arg Asp Ile Asp Ala Ile Ala Val Gln Leu Lys Arg Leu 
310                 315                 320                 325 


Gln Asp Ala Asn Val Pro Val Leu Trp Arg Pro Leu His Glu Ala Glu 
                330                 335                 340     


Gly Gly Trp Phe Trp Trp Gly Ala Lys Gly Pro Glu Pro Ala Lys Gln 
            345                 350                 355         


Leu Tyr Arg Leu Met Tyr Asp Arg Leu Thr His Asp His Asn Leu Asn 
        360                 365                 370             


Asn Leu Ile Trp Ile Trp Asn Ser Glu Lys Lys Asp Trp Tyr Pro Gly 
    375                 380                 385                 


Asp Asp Val Val Asp Ile Val Ser Val Asp Ile Tyr Asn Pro Ala Glu 
390                 395                 400                 405 


Asp Tyr Asn Pro Ser Ile Ala Lys Tyr Glu Gly Leu Val Ser Leu Val 
                410                 415                 420     


Asn Gly Lys Lys Met Ala Ala Leu Ala Glu Asn Gly Pro Ile Pro Asp 
            425                 430                 435         


Pro Asp Ala Leu Gln Ala Tyr Gly Ala Glu Trp Ser Phe Phe Ser Thr 
        440                 445                 450             


Trp Thr Gly Asp Tyr Ile Arg Asp Gly Lys Thr Asn Thr Met Glu His 
    455                 460                 465                 


Leu Lys Lys Val Tyr His His Asp Tyr Val Ile Thr Leu Asp Glu Leu 
470                 475                 480                 485 


Pro Ala Asp Leu Tyr Ala Asn Pro Glu Phe Glu Ala Glu Asn Gly Glu 
                490                 495                 500     


Ser Ala Gly Met Thr Arg Ala Asn Gly Gln Glu Ser His Ser Lys Gly 
            505                 510                 515         


Gly Tyr Thr Thr Gly Met Glu Pro Lys Asn His Pro His Pro His Pro 
        520                 525                 530             


His Pro 
    535 


<210>  14
<211>  27
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Synthetic Construct

<400>  14

Met Lys Lys Pro Leu Gly Lys Ile Val Ala Ser Thr Ala Leu Leu Ile 
1               5                   10                  15      


Ser Val Ala Phe Ser Ser Ser Ile Ala Ser Ala 
            20                  25          


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthethic Construct

<400>  15

His Pro His Pro His Pro His Pro 
1               5               


<210>  16
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  16

Met Asn Met Glu Gly Thr Pro 
1               5           


<210>  17
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  17

Asn Met Glu Gly Thr Pro Ser 
1               5           


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  18

Met Glu Gly Thr Pro Ser Val 
1               5           


