                         SEQUENCE LISTING

<110>  VIB VZW
       VRIJE UNIVERSITEIT BRUSSEL
 
<120>  Compounds to inhibit bacterial S-layer protein assembly

<130>  HaRe/SAPNb/608

<150>  GB 1716059.9
<151>  2017-10-02

<150>  EP 18169187.4
<151>  2018-04-25

<160>  54    

<170>  PatentIn version 3.5

<210>  1
<211>  814
<212>  PRT
<213>  Bacillus anthracis

<400>  1

Met Ala Lys Thr Asn Ser Tyr Lys Lys Val Ile Ala Gly Thr Met Thr 
1               5                   10                  15      


Ala Ala Met Val Ala Gly Val Val Ser Pro Val Ala Ala Ala Gly Lys 
            20                  25                  30          


Thr Phe Pro Asp Val Pro Ala Asp His Trp Gly Ile Asp Ser Ile Asn 
        35                  40                  45              


Tyr Leu Val Glu Lys Gly Ala Val Lys Gly Asn Asp Lys Gly Met Phe 
    50                  55                  60                  


Glu Pro Gly Lys Glu Leu Thr Arg Ala Glu Ala Ala Thr Met Met Ala 
65                  70                  75                  80  


Gln Ile Leu Asn Leu Pro Ile Asp Lys Asp Ala Lys Pro Ser Phe Ala 
                85                  90                  95      


Asp Ser Gln Gly Gln Trp Tyr Thr Pro Phe Ile Ala Ala Val Glu Lys 
            100                 105                 110         


Ala Gly Val Ile Lys Gly Thr Gly Asn Gly Phe Glu Pro Asn Gly Lys 
        115                 120                 125             


Ile Asp Arg Val Ser Met Ala Ser Leu Leu Val Glu Ala Tyr Lys Leu 
    130                 135                 140                 


Asp Thr Lys Val Asn Gly Thr Pro Ala Thr Lys Phe Lys Asp Leu Glu 
145                 150                 155                 160 


Thr Leu Asn Trp Gly Lys Glu Lys Ala Asn Ile Leu Val Glu Leu Gly 
                165                 170                 175     


Ile Ser Val Gly Thr Gly Asp Gln Trp Glu Pro Lys Lys Thr Val Thr 
            180                 185                 190         


Lys Ala Glu Ala Ala Gln Phe Ile Ala Lys Thr Asp Lys Gln Phe Gly 
        195                 200                 205             


Thr Glu Ala Ala Lys Val Glu Ser Ala Lys Ala Val Thr Thr Gln Lys 
    210                 215                 220                 


Val Glu Val Lys Phe Ser Lys Ala Val Glu Lys Leu Thr Lys Glu Asp 
225                 230                 235                 240 


Ile Lys Val Thr Asn Lys Ala Asn Asn Asp Lys Val Leu Val Lys Glu 
                245                 250                 255     


Val Thr Leu Ser Glu Asp Lys Lys Ser Ala Thr Val Glu Leu Tyr Ser 
            260                 265                 270         


Asn Leu Ala Ala Lys Gln Thr Tyr Thr Val Asp Val Asn Lys Val Gly 
        275                 280                 285             


Lys Thr Glu Val Ala Val Gly Ser Leu Glu Ala Lys Thr Ile Glu Met 
    290                 295                 300                 


Ala Asp Gln Thr Val Val Ala Asp Glu Pro Thr Ala Leu Gln Phe Thr 
305                 310                 315                 320 


Val Lys Asp Glu Asn Gly Thr Glu Val Val Ser Pro Glu Gly Ile Glu 
                325                 330                 335     


Phe Val Thr Pro Ala Ala Glu Lys Ile Asn Ala Lys Gly Glu Ile Thr 
            340                 345                 350         


Leu Ala Lys Gly Thr Ser Thr Thr Val Lys Ala Val Tyr Lys Lys Asp 
        355                 360                 365             


Gly Lys Val Val Ala Glu Ser Lys Glu Val Lys Val Ser Ala Glu Gly 
    370                 375                 380                 


Ala Ala Val Ala Ser Ile Ser Asn Trp Thr Val Ala Glu Gln Asn Lys 
385                 390                 395                 400 


Ala Asp Phe Thr Ser Lys Asp Phe Lys Gln Asn Asn Lys Val Tyr Glu 
                405                 410                 415     


Gly Asp Asn Ala Tyr Val Gln Val Glu Leu Lys Asp Gln Phe Asn Ala 
            420                 425                 430         


Val Thr Thr Gly Lys Val Glu Tyr Glu Ser Leu Asn Thr Glu Val Ala 
        435                 440                 445             


Val Val Asp Lys Ala Thr Gly Lys Val Thr Val Leu Ser Ala Gly Lys 
    450                 455                 460                 


Ala Pro Val Lys Val Thr Val Lys Asp Ser Lys Gly Lys Glu Leu Val 
465                 470                 475                 480 


Ser Lys Thr Val Glu Ile Glu Ala Phe Ala Gln Lys Ala Met Lys Glu 
                485                 490                 495     


Ile Lys Leu Glu Lys Thr Asn Val Ala Leu Ser Thr Lys Asp Val Thr 
            500                 505                 510         


Asp Leu Lys Val Lys Ala Pro Val Leu Asp Gln Tyr Gly Lys Glu Phe 
        515                 520                 525             


Thr Ala Pro Val Thr Val Lys Val Leu Asp Lys Asp Gly Lys Glu Leu 
    530                 535                 540                 


Lys Glu Gln Lys Leu Glu Ala Lys Tyr Val Asn Lys Glu Leu Val Leu 
545                 550                 555                 560 


Asn Ala Ala Gly Gln Glu Ala Gly Asn Tyr Thr Val Val Leu Thr Ala 
                565                 570                 575     


Lys Ser Gly Glu Lys Glu Ala Lys Ala Thr Leu Ala Leu Glu Leu Lys 
            580                 585                 590         


Ala Pro Gly Ala Phe Ser Lys Phe Glu Val Arg Gly Leu Glu Lys Glu 
        595                 600                 605             


Leu Asp Lys Tyr Val Thr Glu Glu Asn Gln Lys Asn Ala Met Thr Val 
    610                 615                 620                 


Ser Val Leu Pro Val Asp Ala Asn Gly Leu Val Leu Lys Gly Ala Glu 
625                 630                 635                 640 


Ala Ala Glu Leu Lys Val Thr Thr Thr Asn Lys Glu Gly Lys Glu Val 
                645                 650                 655     


Asp Ala Thr Asp Ala Gln Val Thr Val Gln Asn Asn Ser Val Ile Thr 
            660                 665                 670         


Val Gly Gln Gly Ala Lys Ala Gly Glu Thr Tyr Lys Val Thr Val Val 
        675                 680                 685             


Leu Asp Gly Lys Leu Ile Thr Thr His Ser Phe Lys Val Val Asp Thr 
    690                 695                 700                 


Ala Pro Thr Ala Lys Gly Leu Ala Val Glu Phe Thr Ser Thr Ser Leu 
705                 710                 715                 720 


Lys Glu Val Ala Pro Asn Ala Asp Leu Lys Ala Ala Leu Leu Asn Ile 
                725                 730                 735     


Leu Ser Val Asp Gly Val Pro Ala Thr Thr Ala Lys Ala Thr Val Ser 
            740                 745                 750         


Asn Val Glu Phe Val Ser Ala Asp Thr Asn Val Val Ala Glu Asn Gly 
        755                 760                 765             


Thr Val Gly Ala Lys Gly Ala Thr Ser Ile Tyr Val Lys Asn Leu Thr 
    770                 775                 780                 


Val Val Lys Asp Gly Lys Glu Gln Lys Val Glu Phe Asp Lys Ala Val 
785                 790                 795                 800 


Gln Val Ala Val Ser Ile Lys Glu Ala Lys Pro Ala Thr Lys 
                805                 810                 


<210>  2
<211>  2445
<212>  DNA
<213>  Bacillus anthracis

<400>  2
atggcaaaga ctaactctta caaaaaagta atcgctggta caatgacagc agcaatggta       60

gcaggtgttg tttctccagt agcagcagca ggtaaaacat tcccagacgt tcctgctgat      120

cactggggaa ttgattctat taactactta gtagaaaaag gcgcagttaa aggtaacgac      180

aaaggaatgt tcgagcctgg aaaagaatta actcgtgcag aagcagctac aatgatggct      240

caaatcttaa acttaccaat cgataaagat gctaaaccat ctttcgctga ctctcaaggc      300

caatggtaca ctccattcat cgcagctgta gaaaaagctg gcgttattaa aggtacagga      360

aacggctttg agccaaacgg aaaaatcgac cgcgtttcta tggcatctct tcttgtagaa      420

gcttacaaat tagatactaa agtaaacggt actccagcaa ctaaattcaa agatttagaa      480

acattaaact ggggtaaaga aaaagctaac atcttagttg aattaggaat ctctgttggt      540

actggtgatc aatgggagcc taagaaaact gtaactaaag cagaagctgc tcaattcatt      600

gctaagactg acaagcagtt cggtacagaa gcagcaaaag ttgaatctgc aaaagctgtt      660

acaactcaaa aagtagaagt taaattcagc aaagctgttg aaaaattaac taaagaagat      720

atcaaagtaa ctaacaaagc taacaacgat aaagtactag ttaaagaggt aactttatca      780

gaagataaaa aatctgctac agttgaatta tatagtaact tagcagctaa acaaacttac      840

actgtagatg taaacaaagt tggtaaaaca gaagtagctg taggttcttt agaagcaaaa      900

acaatcgaaa tggctgacca aacagttgta gctgatgagc caacagcatt acaattcaca      960

gttaaagatg aaaacggtac tgaagttgtt tcaccagagg gtattgaatt tgtaacgcca     1020

gctgcagaaa aaattaatgc aaaaggtgaa atcactttag caaaaggtac ttcaactact     1080

gtaaaagctg tttataaaaa agacggtaaa gtagtagctg aaagtaaaga agtaaaagtt     1140

tctgctgaag gtgctgcagt agcttcaatc tctaactgga cagttgcaga acaaaataaa     1200

gctgacttta cttctaaaga tttcaaacaa aacaataaag tttacgaagg cgacaacgct     1260

tacgttcaag tagaattgaa agatcaattt aacgcagtaa caactggaaa agttgaatat     1320

gagtcgttaa acacagaagt tgctgtagta gataaagcta ctggtaaagt aactgtatta     1380

tctgcaggaa aagcaccagt aaaagtaact gtaaaagatt caaaaggtaa agaacttgtt     1440

tcaaaaacag ttgaaattga agctttcgct caaaaagcaa tgaaagaaat taaattagaa     1500

aaaactaacg tagcgctttc tacaaaagat gtaacagatt taaaagtaaa agctccagta     1560

ctagatcaat acggtaaaga gtttacagct cctgtaacag tgaaagtact tgataaagat     1620

ggtaaagaat taaaagaaca aaaattagaa gctaaatatg tgaacaaaga attagttctg     1680

aatgcagcag gtcaagaagc tggtaattat acagttgtat taactgcaaa atctggtgaa     1740

aaagaagcaa aagctacatt agctctagaa ttaaaagctc caggtgcatt ctctaaattt     1800

gaagttcgtg gtttagaaaa agaattagat aaatatgtta ctgaggaaaa ccaaaagaat     1860

gcaatgactg tttcagttct tcctgtagat gcaaatggat tagtattaaa aggtgcagaa     1920

gcagctgaac taaaagtaac aacaacaaac aaagaaggta aagaagtaga cgcaactgat     1980

gcacaagtta ctgtacaaaa taacagtgta attactgttg gtcaaggtgc aaaagctggt     2040

gaaacttata aagtaacagt tgtactagat ggtaaattaa tcacaactca ttcattcaaa     2100

gttgttgata cagcaccaac tgctaaagga ttagcagtag aatttacaag cacatctctt     2160

aaagaagtag ctccaaatgc tgatttaaaa gctgcacttt taaatatctt atctgttgat     2220

ggtgtacctg cgactacagc aaaagcaaca gtttctaatg tagaatttgt ttctgctgac     2280

acaaatgttg tagctgaaaa tggtacagtt ggtgcaaaag gtgcaacatc tatctatgtg     2340

aaaaacctga cagttgtaaa agatggaaaa gagcaaaaag tagaatttga taaagctgta     2400

caagttgcag tttctattaa agaagcaaaa cctgcaacaa aataa                     2445


<210>  3
<211>  2442
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E.coli codon usage optimized B.anthracis sterne SAP coding 
       sequence

<400>  3
atggctaaga cgaatagcta caaaaaagtg attgcgggca ctatgaccgc ggcaatggtc       60

gctggtgtgg tttctccggt agccgcagcc ggtaagacct tcccggacgt tccggcggat      120

cattggggca tcgactctat caattatctc gttgagaagg gcgctgtgaa gggcaatgac      180

aagggcatgt ttgagccagg caaggaactc acgcgcgctg aggcagcaac catgatggcg      240

caaattctga acctgccgat tgataaggac gcaaaacctt ccttcgctga cagccagggc      300

cagtggtaca ccccttttat tgcagcggtg gagaaggcgg gtgtaatcaa aggcactggt      360

aatggctttg aaccgaatgg taagatcgac cgtgtttcta tggcgtctct gctggtcgaa      420

gcgtataaac tcgacaccaa agttaacggt actccggcca cgaagtttaa ggacctggaa      480

accctcaact ggggtaagga aaaggctaac atcctggttg aactcggcat ctctgtgggc      540

acgggcgacc agtgggagcc gaagaaaacc gttaccaagg ctgaagctgc tcagttcatt      600

gcgaagaccg acaagcaatt tggtactgag gctgccaaag tagagtccgc gaaggccgtg      660

acgactcaga aagtggaagt gaagttctct aaggctgtcg agaagctcac gaaagaagat      720

atcaaagtaa cgaacaaggc aaacaacgac aaagtcctcg taaaggaagt tacgctgtcc      780

gaagataaaa aatccgccac cgtggaactg tactctaacc tggcggcgaa gcaaacgtac      840

accgtagacg ttaataaggt tggcaagact gaggtagcag tgggttctct cgaagccaag      900

acgatcgaaa tggcagacca gactgtggtt gccgatgagc cgaccgcact gcaatttacg      960

gtgaaagatg aaaacggcac ggaagttgtt tcccctgagg gtattgagtt tgtgaccccg     1020

gcagctgaaa agatcaatgc taagggcgag attaccctcg cgaaaggtac tagcacgact     1080

gttaaagccg tttacaagaa agacggtaag gtcgtagcgg aatctaaaga agtaaaggta     1140

agcgcggaag gtgcggcagt tgcgtctatt tctaactgga cggttgcgga acagaataaa     1200

gctgacttca cgagcaaaga ctttaagcaa aacaacaagg tctacgaggg tgacaacgcg     1260

tacgtgcagg tagaactgaa agaccaattt aacgcagtta cgaccggtaa agttgaatac     1320

gaatctctga acaccgaagt ggcagtggtg gacaaagcaa ctggcaaggt gactgtgctg     1380

tctgcaggca aagcacctgt aaaagtcacg gttaaagatt ccaagggtaa agaactcgtt     1440

tctaaaactg ttgagatcga agcattcgca cagaaagcga tgaaggaaat taagctggag     1500

aaaacgaatg ttgcgctctc tacgaaggac gtaacggacc tgaaggtcaa agctccggtc     1560

ctggaccagt acggcaaaga attcacggcg ccagttactg tcaaagttct ggataaagat     1620

ggtaaagagc tcaaggagca gaaactggag gcgaagtacg tgaacaaaga gctcgtgctc     1680

aacgcggcag gccaggaggc gggcaattac acggttgttc tcactgcgaa atctggcgag     1740

aaagaggcta aggcaacgct cgcgctggag ctgaaggctc caggcgcgtt ttccaaattc     1800

gaagttcgtg gtctcgaaaa ggagctcgat aagtatgtta ctgaagagaa tcagaagaat     1860

gcgatgaccg taagcgtcct cccggtggac gcgaacggtc tggtgctgaa aggcgcagaa     1920

gcggccgaac tcaaggttac caccaccaat aaagagggca aggaggttga cgcgacggac     1980

gcgcaagtca ctgtacaaaa taactctgtt atcaccgttg gtcagggtgc gaaagcgggc     2040

gaaacttaca aggtaaccgt tgtgctcgac ggcaagctca ttacgacgca tagcttcaaa     2100

gtcgtggaca cggccccaac ggccaaaggc ctggcagtag aatttaccag cacctctctc     2160

aaagaggtgg cccctaatgc ggatctgaaa gcagcactgc tgaacattct cagcgttgac     2220

ggcgtacctg cgaccaccgc caaagcgacc gtatctaacg ttgaattcgt gtctgctgac     2280

actaacgtag tcgcggaaaa tggtaccgtg ggcgctaaag gtgctacttc tatttacgtc     2340

aaaaacctga ctgttgttaa ggatggcaaa gaacaaaagg tagagttcga caaggcggta     2400

caggttgcag tttccattaa ggaggccaaa ccagcaacga aa                        2442


<210>  4
<211>  606
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  SAP amino acid sequence w/o SLH domain (aa 216-814 of SEQ ID 
       NO:1) with C-terminal His6 tag

<400>  4

Met Ser Ala Lys Ala Val Thr Thr Gln Lys Val Glu Val Lys Phe Ser 
1               5                   10                  15      


Lys Ala Val Glu Lys Leu Thr Lys Glu Asp Ile Lys Val Thr Asn Lys 
            20                  25                  30          


Ala Asn Asn Asp Lys Val Leu Val Lys Glu Val Thr Leu Ser Glu Asp 
        35                  40                  45              


Lys Lys Ser Ala Thr Val Glu Leu Tyr Ser Asn Leu Ala Ala Lys Gln 
    50                  55                  60                  


Thr Tyr Thr Val Asp Val Asn Lys Val Gly Lys Thr Glu Val Ala Val 
65                  70                  75                  80  


Gly Ser Leu Glu Ala Lys Thr Ile Glu Met Ala Asp Gln Thr Val Val 
                85                  90                  95      


Ala Asp Glu Pro Thr Ala Leu Gln Phe Thr Val Lys Asp Glu Asn Gly 
            100                 105                 110         


Thr Glu Val Val Ser Pro Glu Gly Ile Glu Phe Val Thr Pro Ala Ala 
        115                 120                 125             


Glu Lys Ile Asn Ala Lys Gly Glu Ile Thr Leu Ala Lys Gly Thr Ser 
    130                 135                 140                 


Thr Thr Val Lys Ala Val Tyr Lys Lys Asp Gly Lys Val Val Ala Glu 
145                 150                 155                 160 


Ser Lys Glu Val Lys Val Ser Ala Glu Gly Ala Ala Val Ala Ser Ile 
                165                 170                 175     


Ser Asn Trp Thr Val Ala Glu Gln Asn Lys Ala Asp Phe Thr Ser Lys 
            180                 185                 190         


Asp Phe Lys Gln Asn Asn Lys Val Tyr Glu Gly Asp Asn Ala Tyr Val 
        195                 200                 205             


Gln Val Glu Leu Lys Asp Gln Phe Asn Ala Val Thr Thr Gly Lys Val 
    210                 215                 220                 


Glu Tyr Glu Ser Leu Asn Thr Glu Val Ala Val Val Asp Lys Ala Thr 
225                 230                 235                 240 


Gly Lys Val Thr Val Leu Ser Ala Gly Lys Ala Pro Val Lys Val Thr 
                245                 250                 255     


Val Lys Asp Ser Lys Gly Lys Glu Leu Val Ser Lys Thr Val Glu Ile 
            260                 265                 270         


Glu Ala Phe Ala Gln Lys Ala Met Lys Glu Ile Lys Leu Glu Lys Thr 
        275                 280                 285             


Asn Val Ala Leu Ser Thr Lys Asp Val Thr Asp Leu Lys Val Lys Ala 
    290                 295                 300                 


Pro Val Leu Asp Gln Tyr Gly Lys Glu Phe Thr Ala Pro Val Thr Val 
305                 310                 315                 320 


Lys Val Leu Asp Lys Asp Gly Lys Glu Leu Lys Glu Gln Lys Leu Glu 
                325                 330                 335     


Ala Lys Tyr Val Asn Lys Glu Leu Val Leu Asn Ala Ala Gly Gln Glu 
            340                 345                 350         


Ala Gly Asn Tyr Thr Val Val Leu Thr Ala Lys Ser Gly Glu Lys Glu 
        355                 360                 365             


Ala Lys Ala Thr Leu Ala Leu Glu Leu Lys Ala Pro Gly Ala Phe Ser 
    370                 375                 380                 


Lys Phe Glu Val Arg Gly Leu Glu Lys Glu Leu Asp Lys Tyr Val Thr 
385                 390                 395                 400 


Glu Glu Asn Gln Lys Asn Ala Met Thr Val Ser Val Leu Pro Val Asp 
                405                 410                 415     


Ala Asn Gly Leu Val Leu Lys Gly Ala Glu Ala Ala Glu Leu Lys Val 
            420                 425                 430         


Thr Thr Thr Asn Lys Glu Gly Lys Glu Val Asp Ala Thr Asp Ala Gln 
        435                 440                 445             


Val Thr Val Gln Asn Asn Ser Val Ile Thr Val Gly Gln Gly Ala Lys 
    450                 455                 460                 


Ala Gly Glu Thr Tyr Lys Val Thr Val Val Leu Asp Gly Lys Leu Ile 
465                 470                 475                 480 


Thr Thr His Ser Phe Lys Val Val Asp Thr Ala Pro Thr Ala Lys Gly 
                485                 490                 495     


Leu Ala Val Glu Phe Thr Ser Thr Ser Leu Lys Glu Val Ala Pro Asn 
            500                 505                 510         


Ala Asp Leu Lys Ala Ala Leu Leu Asn Ile Leu Ser Val Asp Gly Val 
        515                 520                 525             


Pro Ala Thr Thr Ala Lys Ala Thr Val Ser Asn Val Glu Phe Val Ser 
    530                 535                 540                 


Ala Asp Thr Asn Val Val Ala Glu Asn Gly Thr Val Gly Ala Lys Gly 
545                 550                 555                 560 


Ala Thr Ser Ile Tyr Val Lys Asn Leu Thr Val Val Lys Asp Gly Lys 
                565                 570                 575     


Glu Gln Lys Val Glu Phe Asp Lys Ala Val Gln Val Ala Val Ser Ile 
            580                 585                 590         


Lys Glu Ala Lys Pro Ala Thr Lys His His His His His His 
        595                 600                 605     


<210>  5
<211>  1821
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E.coli codon usage optimized B.anthracis sterne SAP coding 
       sequence of SAP encoded by SEQ ID NO:4

<400>  5
atgtccgcga aggccgtgac gactcagaaa gtggaagtga agttctctaa ggctgtcgag       60

aagctcacga aagaagatat caaagtaacg aacaaggcaa acaacgacaa agtcctcgta      120

aaggaagtta cgctgtccga agataaaaaa tccgccaccg tggaactgta ctctaacctg      180

gcggcgaagc aaacgtacac cgtagacgtt aataaggttg gcaagactga ggtagcagtg      240

ggttctctcg aagccaagac gatcgaaatg gcagaccaga ctgtggttgc cgatgagccg      300

accgcactgc aatttacggt gaaagatgaa aacggcacgg aagttgtttc ccctgagggt      360

attgagtttg tgaccccggc agctgaaaag atcaatgcta agggcgagat taccctcgcg      420

aaaggtacta gcacgactgt taaagccgtt tacaagaaag acggtaaggt cgtagcggaa      480

tctaaagaag taaaggtaag cgcggaaggt gcggcagttg cgtctatttc taactggacg      540

gttgcggaac agaataaagc tgacttcacg agcaaagact ttaagcaaaa caacaaggtc      600

tacgagggtg acaacgcgta cgtgcaggta gaactgaaag accaatttaa cgcagttacg      660

accggtaaag ttgaatacga atctctgaac accgaagtgg cagtggtgga caaagcaact      720

ggcaaggtga ctgtgctgtc tgcaggcaaa gcacctgtaa aagtcacggt taaagattcc      780

aagggtaaag aactcgtttc taaaactgtt gagatcgaag cattcgcaca gaaagcgatg      840

aaggaaatta agctggagaa aacgaatgtt gcgctctcta cgaaggacgt aacggacctg      900

aaggtcaaag ctccggtcct ggaccagtac ggcaaagaat tcacggcgcc agttactgtc      960

aaagttctgg ataaagatgg taaagagctc aaggagcaga aactggaggc gaagtacgtg     1020

aacaaagagc tcgtgctcaa cgcggcaggc caggaggcgg gcaattacac ggttgttctc     1080

actgcgaaat ctggcgagaa agaggctaag gcaacgctcg cgctggagct gaaggctcca     1140

ggcgcgtttt ccaaattcga agttcgtggt ctcgaaaagg agctcgataa gtatgttact     1200

gaagagaatc agaagaatgc gatgaccgta agcgtcctcc cggtggacgc gaacggtctg     1260

gtgctgaaag gcgcagaagc ggccgaactc aaggttacca ccaccaataa agagggcaag     1320

gaggttgacg cgacggacgc gcaagtcact gtacaaaata actctgttat caccgttggt     1380

cagggtgcga aagcgggcga aacttacaag gtaaccgttg tgctcgacgg caagctcatt     1440

acgacgcata gcttcaaagt cgtggacacg gccccaacgg ccaaaggcct ggcagtagaa     1500

tttaccagca cctctctcaa agaggtggcc cctaatgcgg atctgaaagc agcactgctg     1560

aacattctca gcgttgacgg cgtacctgcg accaccgcca aagcgaccgt atctaacgtt     1620

gaattcgtgt ctgctgacac taacgtagtc gcggaaaatg gtaccgtggg cgctaaaggt     1680

gctacttcta tttacgtcaa aaacctgact gttgttaagg atggcaaaga acaaaaggta     1740

gagttcgaca aggcggtaca ggttgcagtt tccattaagg aggccaaacc agcaacgaaa     1800

catcaccatc accatcacta a                                               1821


<210>  6
<211>  81
<212>  PRT
<213>  Bacillus anthracis

<400>  6

Met Ser Ala Lys Ala Val Thr Thr Gln Lys Val Glu Val Lys Phe Ser 
1               5                   10                  15      


Lys Ala Val Glu Lys Leu Thr Lys Glu Asp Ile Lys Val Thr Asn Lys 
            20                  25                  30          


Ala Asn Asn Asp Lys Val Leu Val Lys Glu Val Thr Leu Ser Glu Asp 
        35                  40                  45              


Lys Lys Ser Ala Thr Val Glu Leu Tyr Ser Asn Leu Ala Ala Lys Gln 
    50                  55                  60                  


Thr Tyr Thr Val Asp Val Asn Lys Val Gly Lys Thr Glu Val Ala Val 
65                  70                  75                  80  


Gly 
    


<210>  7
<211>  90
<212>  PRT
<213>  Bacillus anthracis

<400>  7

Met Ser Leu Glu Ala Lys Thr Ile Glu Met Ala Asp Gln Thr Val Val 
1               5                   10                  15      


Ala Asp Glu Pro Thr Ala Leu Gln Phe Thr Val Lys Asp Glu Asn Gly 
            20                  25                  30          


Thr Glu Val Val Ser Pro Glu Gly Ile Glu Phe Val Thr Pro Ala Ala 
        35                  40                  45              


Glu Lys Ile Asn Ala Lys Gly Glu Ile Thr Leu Ala Lys Gly Thr Ser 
    50                  55                  60                  


Thr Thr Val Lys Ala Val Tyr Lys Lys Asp Gly Lys Val Val Ala Glu 
65                  70                  75                  80  


Ser Lys Glu Val Lys Val Ser Ala Glu Gly 
                85                  90  


<210>  8
<211>  108
<212>  PRT
<213>  Bacillus anthracis

<400>  8

Met Gly Ala Ala Val Ala Ser Ile Ser Asn Trp Thr Val Ala Glu Gln 
1               5                   10                  15      


Asn Lys Ala Asp Phe Thr Ser Lys Asp Phe Lys Gln Asn Asn Lys Val 
            20                  25                  30          


Tyr Glu Gly Asp Asn Ala Tyr Val Gln Val Glu Leu Lys Asp Gln Phe 
        35                  40                  45              


Asn Ala Val Thr Thr Gly Lys Val Glu Tyr Glu Ser Leu Asn Thr Glu 
    50                  55                  60                  


Val Ala Val Val Asp Lys Ala Thr Gly Lys Val Thr Val Leu Ser Ala 
65                  70                  75                  80  


Gly Lys Ala Pro Val Lys Val Thr Val Lys Asp Ser Lys Gly Lys Glu 
                85                  90                  95      


Leu Val Ser Lys Thr Val Glu Ile Glu Ala Phe Ala 
            100                 105             


<210>  9
<211>  106
<212>  PRT
<213>  Bacillus anthracis

<400>  9

Met Gln Lys Ala Met Lys Glu Ile Lys Leu Glu Lys Thr Asn Val Ala 
1               5                   10                  15      


Leu Ser Thr Lys Asp Val Thr Asp Leu Lys Val Lys Ala Pro Val Leu 
            20                  25                  30          


Asp Gln Tyr Gly Lys Glu Phe Thr Ala Pro Val Thr Val Lys Val Leu 
        35                  40                  45              


Asp Lys Asp Gly Lys Glu Leu Lys Glu Gln Lys Leu Glu Ala Lys Tyr 
    50                  55                  60                  


Val Asn Lys Glu Leu Val Leu Asn Ala Ala Gly Gln Glu Ala Gly Asn 
65                  70                  75                  80  


Tyr Thr Val Val Leu Thr Ala Lys Ser Gly Glu Lys Glu Ala Lys Ala 
                85                  90                  95      


Thr Leu Ala Leu Glu Leu Lys Ala Pro Gly 
            100                 105     


<210>  10
<211>  112
<212>  PRT
<213>  Bacillus anthracis

<400>  10

Met Ala Phe Ser Lys Phe Glu Val Arg Gly Leu Glu Lys Glu Leu Asp 
1               5                   10                  15      


Lys Tyr Val Thr Glu Glu Asn Gln Lys Asn Ala Met Thr Val Ser Val 
            20                  25                  30          


Leu Pro Val Asp Ala Asn Gly Leu Val Leu Lys Gly Ala Glu Ala Ala 
        35                  40                  45              


Glu Leu Lys Val Thr Thr Thr Asn Lys Glu Gly Lys Glu Val Asp Ala 
    50                  55                  60                  


Thr Asp Ala Gln Val Thr Val Gln Asn Asn Ser Val Ile Thr Val Gly 
65                  70                  75                  80  


Gln Gly Ala Lys Ala Gly Glu Thr Tyr Lys Val Thr Val Val Leu Asp 
                85                  90                  95      


Gly Lys Leu Ile Thr Thr His Ser Phe Lys Val Val Asp Thr Ala Pro 
            100                 105                 110         


<210>  11
<211>  109
<212>  PRT
<213>  Bacillus anthracis

<400>  11

Met Thr Ala Lys Gly Leu Ala Val Glu Phe Thr Ser Thr Ser Leu Lys 
1               5                   10                  15      


Glu Val Ala Pro Asn Ala Asp Leu Lys Ala Ala Leu Leu Asn Ile Leu 
            20                  25                  30          


Ser Val Asp Gly Val Pro Ala Thr Thr Ala Lys Ala Thr Val Ser Asn 
        35                  40                  45              


Val Glu Phe Val Ser Ala Asp Thr Asn Val Val Ala Glu Asn Gly Thr 
    50                  55                  60                  


Val Gly Ala Lys Gly Ala Thr Ser Ile Tyr Val Lys Asn Leu Thr Val 
65                  70                  75                  80  


Val Lys Asp Gly Lys Glu Gln Lys Val Glu Phe Asp Lys Ala Val Gln 
                85                  90                  95      


Val Ala Val Ser Ile Lys Glu Ala Lys Pro Ala Thr Lys 
            100                 105                 


<210>  12
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 683

<400>  12

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Lys Met Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys His 
                85                  90                  95      


Ala Asp Phe Ser Thr Gly Trp Ala Pro Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser His His His His His His Glu Pro Glu 
        115                 120                 125             


Ala 
    


<210>  13
<211>  130
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 688

<400>  13

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Arg Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Asp Phe Ser Thr Val Arg Glu Arg Arg Tyr Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Ser Ser His His His His His His Glu Pro 
        115                 120                 125             


Glu Ala 
    130 


<210>  14
<211>  128
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 692

<400>  14

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Val Ile Thr Ser Gly Gly Ser Ala Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Ser Ile Ser Arg Asp Asn Ala Lys Lys Ala Val Tyr Leu 
65                  70                  75                  80  


Arg Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Asp Phe Gly Thr Leu Gly Arg Tyr Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Gln Val Thr Val Ser Ser His His His His His His Glu Pro Glu Ala 
        115                 120                 125             


<210>  15
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 702

<400>  15

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Val Asn 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ile Ile Ile Thr Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Asp Phe Gly Thr Gly Pro Gly Arg Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser His His His His His His Glu Pro Glu 
        115                 120                 125             


Ala 
    


<210>  16
<211>  131
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 704

<400>  16

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Phe 
            20                  25                  30          


Ala Ile Ala Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Asp Ile Asn Ser Gly Gly Thr Asp Thr Ser Tyr Ala Ala Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Ser Ala Gln Ser Tyr Phe His Ser Thr Phe Asp His Arg Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His His Glu 
        115                 120                 125             


Pro Glu Ala 
    130     


<210>  17
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 707

<400>  17

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Ala Met Gly Trp Tyr Arg Gln Ala Ser Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Thr Ser Gly Gly Asn Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Asp Phe Gly Thr Gly Gly Arg Pro Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser His His His His His His Glu Pro Glu 
        115                 120                 125             


Ala 
    


<210>  18
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 684

<400>  18

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Phe Cys Ala Ala Ser Gly Ser Ile Phe Arg Val Asn 
            20                  25                  30          


Ala Met Gly Trp Tyr Arg Gln Val Thr Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Thr Ser Gly Gly Arg Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ile Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Asp Leu Gly Thr Gly Gly Arg Ser Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser His His His His His His Glu Pro Glu 
        115                 120                 125             


Ala 
    


<210>  19
<211>  134
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 694

<400>  19

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Pro Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Asp Ile Asn Ser Ser Gly Thr Thr Tyr Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Thr Glu Gly Lys Tyr Gly Arg Thr Trp Tyr Gly Gln Leu Glu Tyr His 
            100                 105                 110         


Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His 
        115                 120                 125             


His His Glu Pro Glu Ala 
    130                 


<210>  20
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 683

<400>  20

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Lys Met Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys His 
                85                  90                  95      


Ala Asp Phe Ser Thr Gly Trp Ala Pro Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser 
        115                 


<210>  21
<211>  120
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 688

<400>  21

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Arg Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Asp Phe Ser Thr Val Arg Glu Arg Arg Tyr Asp Tyr Trp Gly Gln 
            100                 105                 110         


Gly Thr Gln Val Thr Val Ser Ser 
        115                 120 


<210>  22
<211>  118
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 692

<400>  22

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Val Ile Thr Ser Gly Gly Ser Ala Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Ser Ile Ser Arg Asp Asn Ala Lys Lys Ala Val Tyr Leu 
65                  70                  75                  80  


Arg Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Asp Phe Gly Thr Leu Gly Arg Tyr Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         


Gln Val Thr Val Ser Ser 
        115             


<210>  23
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 702

<400>  23

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Val Asn 
            20                  25                  30          


Asp Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ile Ile Ile Thr Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Asp Phe Gly Thr Gly Pro Gly Arg Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser 
        115                 


<210>  24
<211>  121
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 704

<400>  24

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Phe 
            20                  25                  30          


Ala Ile Ala Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Asp Ile Asn Ser Gly Gly Thr Asp Thr Ser Tyr Ala Ala Ala Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Lys Ser Ala Gln Ser Tyr Phe His Ser Thr Phe Asp His Arg Gly 
            100                 105                 110         


Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120     


<210>  25
<211>  119
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-SAP Nanobody 707

<400>  25

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Ala Met Gly Trp Tyr Arg Gln Ala Ser Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Thr Ser Gly Gly Asn Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Asp Phe Gly Thr Gly Gly Arg Pro Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser 
        115                 


<210>  26
<211>  136
<212>  PRT
<213>  Bacillus anthracis

<400>  26

Met Gln Lys Ala Met Lys Glu Ile Lys Leu Glu Lys Thr Asn Val Ala 
1               5                   10                  15      


Leu Ser Thr Lys Asp Val Thr Asp Leu Lys Val Lys Ala Pro Val Leu 
            20                  25                  30          


Asp Gln Tyr Gly Lys Glu Phe Thr Ala Pro Val Thr Val Lys Val Leu 
        35                  40                  45              


Asp Lys Asp Gly Lys Glu Leu Lys Glu Gln Lys Leu Glu Ala Lys Tyr 
    50                  55                  60                  


Val Asn Lys Glu Leu Val Leu Asn Ala Ala Gly Gln Glu Ala Gly Asn 
65                  70                  75                  80  


Tyr Thr Val Val Leu Thr Ala Lys Ser Gly Glu Lys Glu Ala Lys Ala 
                85                  90                  95      


Thr Leu Ala Leu Glu Leu Lys Ala Pro Gly Ala Phe Ser Lys Phe Glu 
            100                 105                 110         


Val Arg Gly Leu Glu Lys Glu Leu Asp Lys Tyr Val Thr Glu Glu Asn 
        115                 120                 125             


Gln Lys Asn Ala Met Thr Val Ser 
    130                 135     


<210>  27
<211>  862
<212>  PRT
<213>  Bacillus anthracis

<400>  27

Met Ala Lys Thr Asn Ser Tyr Lys Lys Val Ile Ala Gly Thr Met Thr 
1               5                   10                  15      


Ala Ala Met Val Ala Gly Ile Val Ser Pro Val Ala Ala Ala Gly Lys 
            20                  25                  30          


Ser Phe Pro Asp Val Pro Ala Gly His Trp Ala Glu Gly Ser Ile Asn 
        35                  40                  45              


Tyr Leu Val Asp Lys Gly Ala Ile Thr Gly Lys Pro Asp Gly Thr Tyr 
    50                  55                  60                  


Gly Pro Thr Glu Ser Ile Asp Arg Ala Ser Ala Ala Val Ile Phe Thr 
65                  70                  75                  80  


Lys Ile Leu Asn Leu Pro Val Asp Glu Asn Ala Gln Pro Ser Phe Lys 
                85                  90                  95      


Asp Ala Lys Asn Ile Trp Ser Ser Lys Tyr Ile Ala Ala Val Glu Lys 
            100                 105                 110         


Ala Gly Val Val Lys Gly Asp Gly Lys Glu Asn Phe Tyr Pro Glu Gly 
        115                 120                 125             


Lys Ile Asp Arg Ala Ser Phe Ala Ser Met Leu Val Ser Ala Tyr Asn 
    130                 135                 140                 


Leu Lys Asp Lys Val Asn Gly Glu Leu Val Thr Thr Phe Glu Asp Leu 
145                 150                 155                 160 


Leu Asp His Trp Gly Glu Glu Lys Ala Asn Ile Leu Ile Asn Leu Gly 
                165                 170                 175     


Ile Ser Val Gly Thr Gly Gly Lys Trp Glu Pro Asn Lys Ser Val Ser 
            180                 185                 190         


Arg Ala Glu Ala Ala Gln Phe Ile Ala Leu Thr Asp Lys Lys Tyr Gly 
        195                 200                 205             


Lys Lys Asp Asn Ala Gln Ala Tyr Val Thr Asp Val Lys Val Ser Glu 
    210                 215                 220                 


Pro Thr Lys Leu Thr Leu Thr Gly Thr Gly Leu Asp Lys Leu Ser Ala 
225                 230                 235                 240 


Asp Asp Val Thr Leu Glu Gly Asp Lys Ala Val Ala Ile Glu Ala Ser 
                245                 250                 255     


Thr Asp Gly Thr Ser Ala Val Val Thr Leu Gly Gly Lys Val Ala Pro 
            260                 265                 270         


Asn Lys Asp Leu Thr Val Lys Val Lys Asn Gln Ser Phe Val Thr Lys 
        275                 280                 285             


Phe Val Tyr Glu Val Lys Lys Leu Ala Val Glu Lys Leu Thr Phe Asp 
    290                 295                 300                 


Asp Asp Arg Ala Gly Gln Ala Ile Ala Phe Lys Leu Asn Asp Glu Lys 
305                 310                 315                 320 


Gly Asn Ala Asp Val Glu Tyr Leu Asn Leu Ala Asn His Asp Val Lys 
                325                 330                 335     


Phe Val Ala Asn Asn Leu Asp Gly Ser Pro Ala Asn Ile Phe Glu Gly 
            340                 345                 350         


Gly Glu Ala Thr Ser Thr Thr Gly Lys Leu Ala Val Gly Ile Lys Gln 
        355                 360                 365             


Gly Asp Tyr Lys Val Glu Val Gln Val Thr Lys Arg Gly Gly Leu Thr 
    370                 375                 380                 


Val Ser Asn Thr Gly Ile Ile Thr Val Lys Asn Leu Asp Thr Pro Ala 
385                 390                 395                 400 


Ser Ala Ile Lys Asn Val Val Phe Ala Leu Asp Ala Asp Asn Asp Gly 
                405                 410                 415     


Val Val Asn Tyr Gly Ser Lys Leu Ser Gly Lys Asp Phe Ala Leu Asn 
            420                 425                 430         


Ser Gln Asn Leu Val Val Gly Glu Lys Ala Ser Leu Asn Lys Leu Val 
        435                 440                 445             


Ala Thr Ile Ala Gly Glu Asp Lys Val Val Asp Pro Gly Ser Ile Ser 
    450                 455                 460                 


Ile Lys Ser Ser Asn His Gly Ile Ile Ser Val Val Asn Asn Tyr Ile 
465                 470                 475                 480 


Thr Ala Glu Ala Ala Gly Glu Ala Thr Leu Thr Ile Lys Val Gly Asp 
                485                 490                 495     


Val Thr Lys Asp Val Lys Phe Lys Val Thr Thr Asp Ser Arg Lys Leu 
            500                 505                 510         


Val Ser Val Lys Ala Asn Pro Asp Lys Leu Gln Val Val Gln Asn Lys 
        515                 520                 525             


Thr Leu Pro Val Thr Phe Val Thr Thr Asp Gln Tyr Gly Asp Pro Phe 
    530                 535                 540                 


Gly Ala Asn Thr Ala Ala Ile Lys Glu Val Leu Pro Lys Thr Gly Val 
545                 550                 555                 560 


Val Ala Glu Gly Gly Leu Asp Val Val Thr Thr Asp Ser Gly Ser Ile 
                565                 570                 575     


Gly Thr Lys Thr Ile Gly Val Thr Gly Asn Asp Val Gly Glu Gly Thr 
            580                 585                 590         


Val His Phe Gln Asn Gly Asn Gly Ala Thr Leu Gly Ser Leu Tyr Val 
        595                 600                 605             


Asn Val Thr Glu Gly Asn Val Ala Phe Lys Asn Phe Glu Leu Val Ser 
    610                 615                 620                 


Lys Val Gly Gln Tyr Gly Gln Ser Pro Asp Thr Lys Leu Asp Leu Asn 
625                 630                 635                 640 


Val Ser Thr Thr Val Glu Tyr Gln Leu Ser Lys Tyr Thr Ser Asp Arg 
                645                 650                 655     


Val Tyr Ser Asp Pro Glu Asn Leu Glu Gly Tyr Glu Val Glu Ser Lys 
            660                 665                 670         


Asn Leu Ala Val Ala Asp Ala Lys Ile Val Gly Asn Lys Val Val Val 
        675                 680                 685             


Thr Gly Lys Thr Pro Gly Lys Val Asp Ile His Leu Thr Lys Asn Gly 
    690                 695                 700                 


Ala Thr Ala Gly Lys Ala Thr Val Glu Ile Val Gln Glu Thr Ile Ala 
705                 710                 715                 720 


Ile Lys Ser Val Asn Phe Lys Pro Val Gln Thr Glu Asn Phe Val Glu 
                725                 730                 735     


Lys Lys Ile Asn Ile Gly Thr Val Leu Glu Leu Glu Lys Ser Asn Leu 
            740                 745                 750         


Asp Asp Ile Val Lys Gly Ile Asn Leu Thr Lys Glu Thr Gln His Lys 
        755                 760                 765             


Val Arg Val Val Lys Ser Gly Ala Glu Gln Gly Lys Leu Tyr Leu Asp 
    770                 775                 780                 


Arg Asn Gly Asp Ala Val Phe Asn Ala Gly Asp Val Lys Leu Gly Asp 
785                 790                 795                 800 


Val Thr Val Ser Gln Thr Ser Asp Ser Ala Leu Pro Asn Phe Lys Ala 
                805                 810                 815     


Asp Leu Tyr Asp Thr Leu Thr Thr Lys Tyr Thr Asp Lys Gly Thr Leu 
            820                 825                 830         


Val Phe Lys Val Leu Lys Asp Lys Asp Val Ile Thr Ser Glu Ile Gly 
        835                 840                 845             


Ser Gln Ala Val His Val Asn Val Leu Asn Asn Pro Asn Leu 
    850                 855                 860         


<210>  28
<211>  665
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EA1 amino acid sequence w/o SLH domain with N-terminal His6 tag 
       and 10 aa-linker

<400>  28

Met His His His His His His Ile Thr Ser Leu Tyr Lys Lys Ala Gly 
1               5                   10                  15      


Met Ala Tyr Val Thr Asp Val Lys Val Ser Glu Pro Thr Lys Leu Thr 
            20                  25                  30          


Leu Thr Gly Thr Gly Leu Asp Lys Leu Ser Ala Asp Asp Val Thr Leu 
        35                  40                  45              


Glu Gly Asp Lys Ala Val Ala Ile Glu Ala Ser Thr Asp Gly Thr Ser 
    50                  55                  60                  


Ala Val Val Thr Leu Gly Gly Lys Val Ala Pro Asn Lys Asp Leu Thr 
65                  70                  75                  80  


Val Lys Val Lys Asn Gln Ser Phe Val Thr Lys Phe Val Tyr Glu Val 
                85                  90                  95      


Lys Lys Leu Ala Val Glu Lys Leu Thr Phe Asp Asp Asp Arg Ala Gly 
            100                 105                 110         


Gln Ala Ile Ala Phe Lys Leu Asn Asp Glu Lys Gly Asn Ala Asp Val 
        115                 120                 125             


Glu Tyr Leu Asn Leu Ala Asn His Asp Val Lys Phe Val Ala Asn Asn 
    130                 135                 140                 


Leu Asp Gly Ser Pro Ala Asn Ile Phe Glu Gly Gly Glu Ala Thr Ser 
145                 150                 155                 160 


Thr Thr Gly Lys Leu Ala Val Gly Ile Lys Gln Gly Asp Tyr Lys Val 
                165                 170                 175     


Glu Val Gln Val Thr Lys Arg Gly Gly Leu Thr Val Ser Asn Thr Gly 
            180                 185                 190         


Ile Ile Thr Val Lys Asn Leu Asp Thr Pro Ala Ser Ala Ile Lys Asn 
        195                 200                 205             


Val Val Phe Ala Leu Asp Ala Asp Asn Asp Gly Val Val Asn Tyr Gly 
    210                 215                 220                 


Ser Lys Leu Ser Gly Lys Asp Phe Ala Leu Asn Ser Gln Asn Leu Val 
225                 230                 235                 240 


Val Gly Glu Lys Ala Ser Leu Asn Lys Leu Val Ala Thr Ile Ala Gly 
                245                 250                 255     


Glu Asp Lys Val Val Asp Pro Gly Ser Ile Ser Ile Lys Ser Ser Asn 
            260                 265                 270         


His Gly Ile Ile Ser Val Val Asn Asn Tyr Ile Thr Ala Glu Ala Ala 
        275                 280                 285             


Gly Glu Ala Thr Leu Thr Ile Lys Val Gly Asp Val Thr Lys Asp Val 
    290                 295                 300                 


Lys Phe Lys Val Thr Thr Asp Ser Arg Lys Leu Val Ser Val Lys Ala 
305                 310                 315                 320 


Asn Pro Asp Lys Leu Gln Val Val Gln Asn Lys Thr Leu Pro Val Thr 
                325                 330                 335     


Phe Val Thr Thr Asp Gln Tyr Gly Asp Pro Phe Gly Ala Asn Thr Ala 
            340                 345                 350         


Ala Ile Lys Glu Val Leu Pro Lys Thr Gly Val Val Ala Glu Gly Gly 
        355                 360                 365             


Leu Asp Val Val Thr Thr Asp Ser Gly Ser Ile Gly Thr Lys Thr Ile 
    370                 375                 380                 


Gly Val Thr Gly Asn Asp Val Gly Glu Gly Thr Val His Phe Gln Asn 
385                 390                 395                 400 


Gly Asn Gly Ala Thr Leu Gly Ser Leu Tyr Val Asn Val Thr Glu Gly 
                405                 410                 415     


Asn Val Ala Phe Lys Asn Phe Glu Leu Val Ser Lys Val Gly Gln Tyr 
            420                 425                 430         


Gly Gln Ser Pro Asp Thr Lys Leu Asp Leu Asn Val Ser Thr Thr Val 
        435                 440                 445             


Glu Tyr Gln Leu Ser Lys Tyr Thr Ser Asp Arg Val Tyr Ser Asp Pro 
    450                 455                 460                 


Glu Asn Leu Glu Gly Tyr Glu Val Glu Ser Lys Asn Leu Ala Val Ala 
465                 470                 475                 480 


Asp Ala Lys Ile Val Gly Asn Lys Val Val Val Thr Gly Lys Thr Pro 
                485                 490                 495     


Gly Lys Val Asp Ile His Leu Thr Lys Asn Gly Ala Thr Ala Gly Lys 
            500                 505                 510         


Ala Thr Val Glu Ile Val Gln Glu Thr Ile Ala Ile Lys Ser Val Asn 
        515                 520                 525             


Phe Lys Pro Val Gln Thr Glu Asn Phe Val Glu Lys Lys Ile Asn Ile 
    530                 535                 540                 


Gly Thr Val Leu Glu Leu Glu Lys Ser Asn Leu Asp Asp Ile Val Lys 
545                 550                 555                 560 


Gly Ile Asn Leu Thr Lys Glu Thr Gln His Lys Val Arg Val Val Lys 
                565                 570                 575     


Ser Gly Ala Glu Gln Gly Lys Leu Tyr Leu Asp Arg Asn Gly Asp Ala 
            580                 585                 590         


Val Phe Asn Ala Gly Asp Val Lys Leu Gly Asp Val Thr Val Ser Gln 
        595                 600                 605             


Thr Ser Asp Ser Ala Leu Pro Asn Phe Lys Ala Asp Leu Tyr Asp Thr 
    610                 615                 620                 


Leu Thr Thr Lys Tyr Thr Asp Lys Gly Thr Leu Val Phe Lys Val Leu 
625                 630                 635                 640 


Lys Asp Lys Asp Val Ile Thr Ser Glu Ile Gly Ser Gln Ala Val His 
                645                 650                 655     


Val Asn Val Leu Asn Asn Pro Asn Leu 
            660                 665 


<210>  29
<211>  1985
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  E.coli codon usage optimized B.anthracis sterne EA1 coding  
       sequence of EA1

<400>  29
atgcatcatc atcatcatca tattaccagc ctgtataaaa aagcgggcat ggcctacgta       60

accgatgtaa aagtgtccga gcctaccaag ctcacgctca cgggtacggg tctggataaa      120

ctctctgcgg acgacgttac gctcgaaggt gataaggcgg tggccattga ggcaagcacc      180

gacggtacgt ctgcagtcgt gaccctcggc ggtaaggttg ccccaaataa ggatctcacc      240

gtgaaagtta agaatcagtc cttcgttact aagtttgttt acgaggtaaa gaagctggcg      300

gtagaaaagc tgacctttga tgatgaccgc gcaggccaag ccatcgcatt caaactcaat      360

gatgaaaaag gcaacgcgga tgtagagtac ctgaacctcg cgaatcatga cgtaaaattc      420

gttgcaaaca acctcgatgg cagccctgca aatatctttg aaggtggcga ggcgacctcc      480

acgaccggta aactcgcggt cggcatcaaa caaggtgact ataaagtcga agtacaagta      540

actaagcgtg gtggcctgac tgtctctaac acgggcatta ttactgtgaa gaatctcgac      600

acgcctgcaa gcgcaatcaa gaacgtagtt tttgctctcg atgcggacaa tgacggcgtg      660

gttaactacg gctctaaact gtctggtaag gatttcgcac tgaacagcca aaacctcgtg      720

gttggcgaga aagcctctct caataagctc gtagcgacta tcgcaggcga agacaaagta      780

gtggatcctg gttccatcag cattaaaagc tctaatcatg gtattatctc cgtagtgaat      840

aactacatca cggcggaggc agccggcgaa gctacgctga cgattaaagt aggcgacgtg      900

accaaagacg ttaaattcaa agttacgacg gattcccgta aactggtgtc tgtgaaagca      960

aacccggaca aactgcaagt tgtacagaat aaaaccctgc cagttacgtt tgtgactacc     1020

gatcagtacg gcgacccatt cggcgcgaac actgctgcga tcaaggaggt actgccgaaa     1080

accggtgtgg tagcagaggg tggtctcgac gtggtcacca cggactctgg ttctattggt     1140

acgaagacga ttggcgtgac gggtaatgac gtgggcgaag gtaccgttca cttccagaat     1200

ggtaatggtg ccacgctggg tagcctgtat gtaaacgtta ccgagggcaa tgtggccttc     1260

aagaatttcg aactcgtttc taaggtaggc cagtatggtc agtctccgga taccaaactc     1320

gacctcaacg tcagcaccac cgtcgaatac caactcagca aatacacgtc tgatcgtgta     1380

tactctgatc cagaaaacct ggagggctat gaagtggagt ctaagaacct ggcagtagcg     1440

gacgcgaaaa tcgtgggtaa taaggtggtg gttacgggca aaaccccagg caaggtcgac     1500

atccacctca ctaagaatgg cgctaccgct ggtaaagcga ctgttgagat tgtgcaggaa     1560

acgattgcca tcaagtctgt taatttcaaa ccagtacaga cggagaactt tgtagagaaa     1620

aagatcaaca tcggcactgt gctggagctg gaaaagagca atctggatga catcgttaaa     1680

ggtattaatc tcacgaaaga aacccagcat aaagtgcgtg ttgttaaatc tggtgcggaa     1740

cagggtaagc tgtacctgga ccgtaacggc gatgctgttt tcaatgcggg cgatgttaag     1800

ctgggtgatg tgactgtttc tcagacctct gactctgcgc tgcctaactt caaggcggac     1860

ctctatgata ccctgaccac taagtatacc gataagggca ctctcgtgtt taaggttctg     1920

aaggacaagg acgtcatcac gagcgaaatt ggctctcagg cagtgcacgt taatgtactg     1980

aataa                                                                 1985


<210>  30
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  30
ggggacaagt ttgtacaaaa aagcaggctt cgaaggagat agaaccatgt ccgcgaaggc       60

cgtg                                                                    64


<210>  31
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  31
ggggaccact ttgtacaaga aagctgggtc ctagtgatgg tgatggtgat gacccactgc       60

tacctcagt                                                               69


<210>  32
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  32
ggggacaagt ttgtacaaaa aagcaggctt cgaaggagat agaaccatgc agaaagcgat       60

gaaggaa                                                                 67


<210>  33
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  33
ggggaccact ttgtacaaga aagctgggtc ctagtgatgg tgatggtgat ggcctggagc       60

cttcagctc                                                               69


<210>  34
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  34
ggggacaagt ttgtacaaaa aagcaggctt cgaaggagat agaaccatgg ccaaaggcct       60

ggcagta                                                                 67


<210>  35
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  35
ggggaccact ttgtacaaga aagctgggtc ctagtgatgg tgatggtgat g                51


<210>  36
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Sap Nanobody 679

<400>  36

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Val Asn 
            20                  25                  30          


Ala Met Gly Trp Tyr Arg Gln Val Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala His Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys His 
                85                  90                  95      


Ala Asp Leu Gly Thr Gly Pro Leu Pro Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser His His His His His His Glu Pro Glu 
        115                 120                 125             


Ala 
    


<210>  37
<211>  129
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Sap Nanobody 687

<400>  37

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Arg Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Ala Ile Thr Ser Gly Gly Ser Thr Asn Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Arg Thr Val Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys Asn 
                85                  90                  95      


Ala Asp Phe Ser Thr Gly Trp Ala Pro Tyr Asp Tyr Trp Gly Gln Gly 
            100                 105                 110         


Thr Gln Val Thr Val Ser Ser His His His His His His Glu Pro Glu 
        115                 120                 125             


Ala 
    


<210>  38
<211>  133
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Sap Nanobody 695

<400>  38

Gln Val His Leu Val Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Ser Ile Phe Arg Ile Asn 
            20                  25                  30          


Phe Met Gly Trp Tyr Arg Gln Ala Pro Gly Lys Gln Arg Glu Leu Val 
        35                  40                  45              


Ala Gly Ile Thr Ser Gly Ser Asn Thr Asn Tyr Ala Thr Asn Tyr Ala 
    50                  55                  60                  


Asp Ser Val Lys Gly Arg Phe Thr Ile Ala Arg Asp Lys Ser Lys Asn 
65                  70                  75                  80  


Thr Val Tyr Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val 
                85                  90                  95      


Tyr Tyr Cys Asn Ala Asp Phe Gly Thr Ser Gly Arg Pro Tyr Phe Tyr 
            100                 105                 110         


Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His His His 
        115                 120                 125             


His Glu Pro Glu Ala 
    130             


<210>  39
<211>  135
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  anti-Sap Nanobody 703

<400>  39

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ser Asp Ile Asn Ser Gly Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Thr Leu Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Ser Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Thr Gly Gly Lys Tyr Gly Ser Thr Trp Tyr Gly Gln Ile Glu Tyr 
            100                 105                 110         


Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His His His 
        115                 120                 125             


His His His Glu Pro Glu Ala 
    130                 135 


<210>  40
<211>  137
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Non-Sap binding control Nb 11

<400>  40

Gln Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Ala Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Arg Thr Phe Ser Ser Phe 
            20                  25                  30          


Ala Met Gly Trp Phe Arg Leu Ala Pro Gly Ala Glu Arg Glu Phe Val 
        35                  40                  45              


Gly Ala Ile Ser Arg Ser Gly Asp Ser Thr Tyr Tyr Ala Glu Ser Val 
    50                  55                  60                  


Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Met Asn Thr Val Tyr 
65                  70                  75                  80  


Leu Gln Met Asn Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Ala Glu Glu Ala Tyr Gly Ser Met Leu Arg Leu Arg Gln Ala Arg 
            100                 105                 110         


Phe Tyr Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser His 
        115                 120                 125             


His His His His His Glu Pro Glu Ala 
    130                 135         


<210>  41
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb 694 CDR3

<400>  41

Gly Lys Tyr Gly Arg Thr Trp Tyr Gly Gln Leu Glu 
1               5                   10          


<210>  42
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb684,683,688,692,702,707 CDR1

<400>  42

Ser Gly Ser Ile Phe Arg 
1               5       


<210>  43
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb684 CDR3

<400>  43

Leu Gly Thr Gly Gly Arg Ser Tyr Asp Tyr Trp 
1               5                   10      


<210>  44
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb683 CDR3

<400>  44

Phe Ser Thr Gly Trp Ala Pro Tyr Asp Tyr Trp 
1               5                   10      


<210>  45
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb688 CDR3

<400>  45

Phe Ser Thr Val Arg Glu Arg Arg Tyr Asp Tyr Trp 
1               5                   10          


<210>  46
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb692 CDR3

<400>  46

Phe Gly Thr Leu Gly Arg Tyr Asp Tyr Trp 
1               5                   10  


<210>  47
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb702 CDR3

<400>  47

Phe Gly Thr Gly Pro Gly Arg Tyr Asp Tyr Trp 
1               5                   10      


<210>  48
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb707 CDR3

<400>  48

Phe Gly Thr Gly Gly Arg Pro Tyr Asp Tyr Trp 
1               5                   10      


<210>  49
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nb704 CDR3

<400>  49

Ser Ala Gln Ser Tyr Phe His Ser Thr Phe Asp His Arg 
1               5                   10              


<210>  50
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sap epitope in D1

<400>  50

Tyr Ser Asn Leu Ala Ala 
1               5       


<210>  51
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sap epitope in D2

<400>  51

Ala Leu Gln Phe Thr Val Lys Asp Glu Asn Gly Thr Glu Val Val Ser 
1               5                   10                  15      


Pro Glu 
        


<210>  52
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  paratope in CDR1

<400>  52

Ser Gly Ser Ile Phe Arg 
1               5       


<210>  53
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  paratope in CDR3

<400>  53

Tyr Asp Tyr Trp 
1               


<210>  54
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His6-EPEA tag

<400>  54

His His His His His His Glu Pro Glu Ala 
1               5                   10  


