                         SEQUENCE LISTING

<110>  The University of York
 
<120>  Bioconjugation of Polypeptides

<130>  P031282WO

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  253
<212>  PRT
<213>  Artificial

<220>
<223>  Mutated GFP(Y39TAG) protein


<220>
<221>  X
<222>  (48)..(48)
<223>  X= thiazolidine-substituted pyrrolysine

<400>  1

Met Asp Tyr Lys Asp Asp Asp Asp Lys Val Ser Lys Gly Glu Glu Leu 
1               5                   10                  15      


Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 
            20                  25                  30          


Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Xaa 
        35                  40                  45              


Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 
    50                  55                  60                  


Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe 
65                  70                  75                  80  


Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 
                85                  90                  95      


Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp 
            100                 105                 110         


Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
        115                 120                 125             


Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 
    130                 135                 140                 


Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr 
145                 150                 155                 160 


Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile 
                165                 170                 175     


Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln 
            180                 185                 190         


Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 
        195                 200                 205             


Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg 
    210                 215                 220                 


Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu 
225                 230                 235                 240 


Gly Met Asp Glu Leu Tyr Lys His His His His His His 
                245                 250             


<210>  2
<211>  253
<212>  PRT
<213>  Artificial

<220>
<223>  mutated GFP protein (S-GFP(Y39TAG)


<220>
<221>  X
<222>  (48)..(48)
<223>  X= thiazolidine substituted pyrrolysine

<400>  2

Met Ser Tyr Lys Asp Asp Asp Asp Lys Val Ser Lys Gly Glu Glu Leu 
1               5                   10                  15      


Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn 
            20                  25                  30          


Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Xaa 
        35                  40                  45              


Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val 
    50                  55                  60                  


Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe 
65                  70                  75                  80  


Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe Phe Lys Ser Ala 
                85                  90                  95      


Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Phe Phe Lys Asp Asp 
            100                 105                 110         


Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu 
        115                 120                 125             


Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn 
    130                 135                 140                 


Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr 
145                 150                 155                 160 


Ile Met Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile 
                165                 170                 175     


Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln 
            180                 185                 190         


Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His 
        195                 200                 205             


Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg 
    210                 215                 220                 


Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu 
225                 230                 235                 240 


Gly Met Asp Glu Leu Tyr Lys His His His His His His 
                245                 250             


<210>  3
<211>  247
<212>  PRT
<213>  Artificial

<220>
<223>  mutated super folded GFP (N150TAG)


<220>
<221>  X
<222>  (150)..(150)
<223>  X= thiazolidine-substituted pyrrolysine

<400>  3

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Asn Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Ser Phe Lys Asp Asp Gly Thr Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Phe Asn Ser His Xaa Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys Gly 
225                 230                 235                 240 


Ser His His His His His His 
                245         


<210>  4
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  4

Leu Tyr Arg Ala Gly 
1               5   


<210>  5
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  Peptide

<400>  5

Ser Leu Tyr Arg Ala Gly 
1               5       


<210>  6
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  6
cactttatca tcatcatctt tgtaagacat ggttaattcc tcctgttagc cc               52


<210>  7
<211>  52
<212>  DNA
<213>  Artificial

<220>
<223>  Primer

<400>  7
gggctaacag gaggaattaa ccatgtctta caaagatgat gatgataaag tg               52


