               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> ENZYMATIC REMOVAL OF CHLOROPHYLL SUBSTRATES FROM TRIACYLGLYCEROL-BASED OILS

<130> 32521-WO-PCT[2]


<150> EP17193211.4
<151> 2017-09-26
<150> US62/736129
<151> 2018-09-25
<150> US62/736126
<151> 2018-09-25

<160> 14

<170> BiSSAP 1.3.6

<210> 1
<211> 318
<212> PRT
<213> Hordeum vulgare


<220> 
<223> CHL26 putative chlorophyllase

<400> 1
Met Ala Ser Ala Gly Asp Val Phe Asp His Gly Arg His Gly Thr Ser 
1               5                   10                  15      
Leu Ala Arg Val Glu Gln Ala Lys Asn Thr Arg Cys Ser Ala Ala Ser 
            20                  25                  30          
Arg Val Asp Ala Asp Ala Gln Ala Gln Gln Ser Pro Pro Lys Pro Leu 
        35                  40                  45              
Leu Val Ala Ala Pro Cys Asp Ala Gly Glu Tyr Pro Val Val Val Phe 
    50                  55                  60                  
Leu His Gly Tyr Leu Cys Asn Asn Tyr Phe Tyr Ser Gln Leu Ile Gln 
65                  70                  75                  80  
His Val Ala Ser His Gly Phe Ile Val Val Cys Pro Gln Leu Tyr Thr 
                85                  90                  95      
Val Ser Gly Pro Asp Thr Thr Ser Glu Ile Asn Ser Ala Ala Ala Val 
            100                 105                 110         
Ile Asp Trp Leu Ala Ala Gly Leu Ser Ser Lys Leu Ala Pro Gly Ile 
        115                 120                 125             
Arg Pro Asn Leu Ala Ala Val Ser Ile Ser Gly His Ser Arg Gly Gly 
    130                 135                 140                 
Lys Val Ala Phe Ala Leu Gly Leu Gly His Ala Lys Thr Ser Leu Pro 
145                 150                 155                 160 
Leu Ala Ala Leu Ile Ala Val Asp Pro Val Asp Gly Thr Gly Met Gly 
                165                 170                 175     
Asn Gln Thr Pro Pro Pro Ile Leu Ala Tyr Lys Pro Asn Ala Ile Arg 
            180                 185                 190         
Val Pro Ala Pro Val Met Val Ile Gly Thr Gly Leu Gly Glu Leu Pro 
        195                 200                 205             
Arg Asn Ala Leu Phe Pro Pro Cys Ala Pro Leu Gly Val Ser His Ala 
    210                 215                 220                 
Ala Phe Tyr Asp Glu Cys Ala Ala Pro Ala Cys His Leu Val Ala Arg 
225                 230                 235                 240 
Asp Tyr Gly His Thr Asp Met Met Asp Asp Val Thr Thr Gly Ala Lys 
                245                 250                 255     
Gly Leu Ala Thr Arg Ala Leu Cys Lys Ser Gly Gly Ala Arg Ala Pro 
            260                 265                 270         
Met Arg Arg Phe Val Ala Gly Ala Met Val Ala Phe Leu Asn Lys Trp 
        275                 280                 285             
Val Glu Gly Lys Pro Glu Trp Leu Asp Ala Val Arg Glu Gln Thr Val 
    290                 295                 300                 
Ala Ala Pro Val Val Leu Ser Ala Val Glu Phe Arg Asp Glu 
305                 310                 315             

<210> 2
<211> 957
<212> DNA
<213> Artificial Sequence


<220> 
<223> Codon optimized nucleic acid sequence encoding a putative
      chlorophyllase from Hordeum vulgare CHL26

<400> 2
atggcatcag caggggacgt atttgatcat ggacggcacg gaaccagttt ggcgagggta      60

gaacaagcga agaacacccg ctgtagcgca gcgtcccgcg tggatgcgga cgcccaggct     120

cagcagtcgc cgcccaaacc cctcctggtt gcagccccat gcgacgcagg cgaatacccg     180

gtggtcgtat tccttcacgg ctacttgtgc aacaattact tctactcgca gctgatccag     240

catgtcgcga gccacggctt cattgtagtg tgcccgcagc tgtataccgt gtctggtccg     300

gatacaacca gtgaaattaa tagcgccgct gccgtcatcg actggctcgc ggcaggactg     360

tcgtccaagc tggccccagg catccgtccg aacctggccg ccgtgagcat cagcggccac     420

tcacgcggtg gcaaggtggc ctttgccctg ggtctggggc atgccaagac cagcttgccg     480

ctggctgccc tgattgccgt ggatccagtc gacggcaccg ggatgggcaa ccaaactccc     540

cctccgatcc tggcctataa gccgaacgcc attcgggtcc ctgctcccgt gatggtgatc     600

ggcacaggac tgggcgaact gcctcgcaac gcgctgtttc caccttgcgc ccccttgggt     660

gtgtcgcacg ccgcgttcta cgatgagtgt gccgcacccg catgccacct ggttgcccgc     720

gactatggcc acaccgacat gatggatgac gtaacaaccg gcgccaaagg cctggcaacc     780

cgtgcgctgt gcaagagcgg tggggcaaga gccccgatgc gccgttttgt cgccggcgct     840

atggtggcgt tcctgaataa gtgggtggag ggcaagccgg aatggttgga cgccgtgcgc     900

gaacagaccg tggctgcacc ggtggtcctg tccgccgtag agtttcgcga tgagtaa        957


<210> 3
<211> 309
<212> PRT
<213> Gossypium raimondii


<220> 
<223> CHL25 putative chlorophyllase

<400> 3
Met Ser Ala Pro Thr Ser Leu Ala Thr Asn Val Phe Gly Phe Gly Ser 
1               5                   10                  15      
Tyr Thr Thr Met Leu Gln Lys Val Glu Ser Val Thr Thr Ser Ser Met 
            20                  25                  30          
Pro Val Pro Pro Pro Lys Ser Leu Leu Ile Ala Thr Pro Ser Glu Ala 
        35                  40                  45              
Gly Glu Phe Pro Leu Leu Ile Phe Leu His Gly Tyr Leu Leu Tyr Asn 
    50                  55                  60                  
Ser Phe Tyr Ser Gln Leu Leu Gln His Val Ala Ser His Gly Phe Ile 
65                  70                  75                  80  
Val Ile Ala Pro Gln Leu Tyr Ile Val Ala Gly Pro Asp Thr Thr Asp 
                85                  90                  95      
Glu Ile Lys Ser Thr Ala Ala Ile Thr Asn Trp Leu Ser Lys Gly Val 
            100                 105                 110         
Leu Gln Gly Leu Leu Pro Pro Tyr Val Arg Pro Asn Leu Ser Lys Leu 
        115                 120                 125             
Ala Leu Ala Gly His Ser Arg Gly Gly Lys Val Ala Phe Ala Leu Ala 
    130                 135                 140                 
Leu Gln Lys Thr Met Thr Lys Leu Lys Phe Ser Thr Leu Ile Gly Ile 
145                 150                 155                 160 
Asp Pro Val Asp Gly Met Asp Lys Gly Lys Gln Thr Pro Pro Pro Val 
                165                 170                 175     
Leu Thr Tyr Ile Pro His Ser Phe Asp Leu Asp Met Ala Val Met Val 
            180                 185                 190         
Ile Gly Ser Gly Leu Gly Glu Val Lys Arg Asn Pro Leu Phe Pro Pro 
        195                 200                 205             
Cys Ala Pro Lys Gly Val Asn His Glu Asp Phe Phe Lys Glu Cys Arg 
    210                 215                 220                 
Lys Pro Ala Cys His Ile Val Ala Lys Asp Tyr Gly His Leu Asp Met 
225                 230                 235                 240 
Leu Asp Asp Glu Thr Asn Gly Phe Arg Gly Arg Ser Ser Tyr Cys Leu 
                245                 250                 255     
Cys Lys Asn Gly Glu Ala Arg Glu Pro Met Arg Arg Phe Val Gly Gly 
            260                 265                 270         
Val Val Val Ala Ser Met Lys Ala Tyr Leu Asn Gly Asp Asn Thr Asp 
        275                 280                 285             
Leu Ile Ala Ile Lys Gly His Glu Ala Ala Pro Val Glu Leu Lys Thr 
    290                 295                 300                 
Ile Glu Phe Leu Val 
305                 

<210> 4
<211> 306
<212> PRT
<213> Phoenix dactylifera


<220> 
<223> CHL27 putative chlorophyllase

<400> 4
Met Ala Ser Val Ile Ile Asn Lys Pro Ser Ala Thr Ala Ala Ser Val 
1               5                   10                  15      
Phe Asp Tyr Gly Lys Leu His Val Asp Thr Ile Pro Ala Lys Gln Ser 
            20                  25                  30          
Asp Glu Ser Ser Pro Pro Lys Asp Ile Leu Val Val Cys Pro Lys Val 
        35                  40                  45              
Ala Gly Ser Tyr Thr Val Val Leu Phe Ile Gln Gly Tyr Leu Leu Ser 
    50                  55                  60                  
Asn Ala Tyr Tyr Thr Gln Leu Leu Gln His Val Ala Ser His Gly Phe 
65                  70                  75                  80  
Ile Leu Ile Ala Pro Gln Cys Ile Val Val Ser Pro Tyr Ser Glu Glu 
                85                  90                  95      
Asp Ile Thr Ser Ala Ala Ala Val Thr Asn Trp Leu Ser Asp Gly Leu 
            100                 105                 110         
Gln Ser Val Leu Pro Thr Gly Val Glu Ala Asn Leu Asp Lys Leu Ala 
        115                 120                 125             
Leu Ala Gly His Ser Arg Gly Gly His Ala Ala Phe Ala Leu Ala Leu 
    130                 135                 140                 
Gly His Ala Glu Thr Thr Leu Lys Phe Ser Leu Leu Met Gly Ile Asp 
145                 150                 155                 160 
Pro Val Ala Gly Pro Ser Lys Cys Cys Gln Ile Pro Pro Lys Ile Leu 
                165                 170                 175     
Thr Tyr Glu Pro Ser Ser Phe Glu Leu Glu Ile Pro Val Leu Val Leu 
            180                 185                 190         
Gly Thr Gly Leu Gly Ser Glu Gln Lys Asn Ile Leu Phe Pro Ala Cys 
        195                 200                 205             
Ala Pro Asp Gly Val Asn His Lys Glu Phe Tyr Ser Glu Cys Lys Pro 
    210                 215                 220                 
Pro Cys Tyr His Phe Val Val Thr Asp Tyr Gly His Leu Asp Met Leu 
225                 230                 235                 240 
Asp Asp Thr Ala Pro Lys Ile Thr Lys Cys Val Cys Lys Asn Gly Thr 
                245                 250                 255     
Asn Cys Arg Glu Ile Met Arg Arg Thr Thr Gly Gly Ile Met Thr Ala 
            260                 265                 270         
Phe Leu Lys Ala Tyr Leu Leu Asp Leu Glu Glu Asp Leu Lys Ala Ile 
        275                 280                 285             
Ala Asp Pro Gln Ile Ala Pro Thr Lys Leu Asp Pro Val Ser Tyr Arg 
    290                 295                 300                 
Leu Glu 
305     

<210> 5
<211> 326
<212> PRT
<213> Phoenix dactylifera


<220> 
<223> CHL28 putative chlorophyllase

<400> 5
Met Gly Leu Glu Asp Ile Phe Lys Glu Gly Pro Leu Pro Ile Gln Thr 
1               5                   10                  15      
Leu Thr Ile Pro Ala Gln Gln Arg Ala Thr Ala Thr Gly Pro Cys Cys 
            20                  25                  30          
His Gly Arg Ala Ser Pro Met Glu Pro Thr Ala Leu Pro Pro Lys Pro 
        35                  40                  45              
Leu Met Val Ile Leu Pro Ser Gln Glu Gly Asp Tyr Pro Val Leu Leu 
    50                  55                  60                  
Tyr Leu His Gly Tyr Leu Leu Leu Asn Ser Tyr Tyr Ser Gln Leu Leu 
65                  70                  75                  80  
Cys His Ile Ala Ser His Gly Tyr Ile Ala Ile Ala Pro Gln Met Tyr 
                85                  90                  95      
Thr Ala Ala Gly Pro Asp Ala Thr Pro Glu Ile Arg Asp Ala Val Ala 
            100                 105                 110         
Ile Thr Glu Trp Leu Pro Thr Gly Phe Ala Gly Arg Leu Pro Thr His 
        115                 120                 125             
Val Arg Pro Asp Leu Gln Lys Val Ala Val Ala Gly His Ser Arg Gly 
    130                 135                 140                 
Gly Lys Val Ala Phe Gly Ala Ala Leu Gly Arg Ala Thr Pro Pro Pro 
145                 150                 155                 160 
Ser Leu Pro Tyr Ala Ala Ile Val Gly Val Asp Pro Val Asp Gly Met 
                165                 170                 175     
Ala Ala Gly Arg Gln Thr Pro Pro Leu Ile Leu Gly Tyr Gly Asp His 
            180                 185                 190         
Asp Phe Glu Asn Ser Ile Pro Ala Leu Val Ile Gly Ser Gly Leu Gly 
        195                 200                 205             
Pro Val Arg Arg Asn Pro Leu Phe Pro Pro Cys Ala Pro Ala Gly Val 
    210                 215                 220                 
Asn His Val Asp Phe Phe Arg Glu Cys Arg Ala Pro Ala Tyr His Phe 
225                 230                 235                 240 
Val Ala Thr Glu Tyr Gly His Gln Asp Phe Leu Asp Asp Glu Thr Gly 
                245                 250                 255     
Gly Val Arg Gly Arg Ala Thr Tyr Cys Leu Cys Lys Asn Gly Thr Ala 
            260                 265                 270         
Arg Glu Pro Met Arg Arg Phe Ala Ala Gly Ile Ile Val Ala Phe Leu 
        275                 280                 285             
Asn Ala Trp Leu Arg Asn Asp Ser Ala Asp Leu Glu Asp Val Leu Asp 
    290                 295                 300                 
Pro Ser Arg Ala Pro Val Lys Met Glu Pro Pro Glu Trp Asn Leu Phe 
305                 310                 315                 320 
Gln Lys Val Pro Thr Leu 
                325     

<210> 6
<211> 328
<212> PRT
<213> Cucumis sativus


<220> 
<223> CHL29 putative chlorophyllase

<400> 6
Met Ala Ala Val Val Val Asp Val Lys Gly Lys Ser Lys Ser Ala Ala 
1               5                   10                  15      
Val Ser Ser Val Val Asp Leu Asp Pro His Pro Tyr Asp Gly Val Phe 
            20                  25                  30          
Glu Lys Gly Lys Phe Glu Val Gly Val Ile Thr Lys Thr Thr Asp Ile 
        35                  40                  45              
Phe Ser Thr Ser Lys Pro Leu Leu Val Phe Thr Pro Lys Thr Pro Gly 
    50                  55                  60                  
Leu Tyr Pro Ala Ile Leu Phe Phe His Gly Phe Ser Cys Tyr Gly Ser 
65                  70                  75                  80  
Phe Tyr Thr Asp Phe Leu Thr Leu Ile Ala Ser His Gly Tyr Val Ile 
                85                  90                  95      
Ala Ala Pro Gln Leu Tyr Val Met Pro Thr Thr Ser Glu Met Asp Glu 
            100                 105                 110         
Ile Lys Ser Ala Val Asp Val Ile Lys Trp Leu Ser Ser Gly Leu Asp 
        115                 120                 125             
Pro Leu Leu Pro Thr Asn Val Lys Gly Asp Leu Ser Lys Leu Ser Leu 
    130                 135                 140                 
Leu Gly His Ser Arg Gly Gly Lys Thr Ala Phe Ser Leu Ala Leu Gly 
145                 150                 155                 160 
Trp Gly Ser Pro Ser Leu Pro Phe Ser Ala Ile Ile Gly Ile Asp Pro 
                165                 170                 175     
Val Ala Gly Ser Lys Phe Phe Arg Pro Glu Pro Gln Ile Leu Asp Pro 
            180                 185                 190         
Pro Ser Gln Pro Phe Lys Ile Ser Leu Pro Ile Thr Val Val Gly Thr 
        195                 200                 205             
Gly Leu Gly Pro Gln Lys Ala Thr Pro Val Thr Cys Ala Cys Ala Pro 
    210                 215                 220                 
Asp Gly Leu Asn His Ile Ala Phe Phe Lys Lys Cys Lys Pro Thr Cys 
225                 230                 235                 240 
Ala His Phe Val Ala Val Asn Tyr Gly His Met Asp Ile Leu Asp Asp 
                245                 250                 255     
Asn Pro Pro Gly Met Thr Gly Tyr Phe Thr Asn Ile Ala Cys Lys Asn 
            260                 265                 270         
Gly Lys Gly Pro Arg Asp Leu Met Arg Lys Cys Cys Ser Gly Leu Val 
        275                 280                 285             
Val Ala Ser Leu Lys Ala Tyr Leu Asp Asn Asp Val Ser Ile Leu Asn 
    290                 295                 300                 
Ala Ile Tyr Asp Pro Ser Ile Ala Pro Thr Glu Leu Asn Pro Val Glu 
305                 310                 315                 320 
Val Ile Tyr Lys Thr Pro Ser Ala 
                325             

<210> 7
<211> 317
<212> PRT
<213> Tarenaya hassleriana


<220> 
<223> CHL30 putative chlorophyllase

<400> 7
Met Gly Glu Glu Ser Glu Arg Leu Gly Ser Ser Ala Phe Glu Pro Gly 
1               5                   10                  15      
Ser Leu Pro Thr Thr Val Ile Lys Ala Asp Pro Ser Arg Glu Asp Pro 
            20                  25                  30          
Val Ser Pro Pro Lys Pro Val Met Ile Val Ala Pro Thr Val Ala Gly 
        35                  40                  45              
Thr Tyr Pro Val Val Phe Phe Phe His Gly Phe Tyr Leu Arg Asn Tyr 
    50                  55                  60                  
Phe Tyr Ser Asp Val Leu Ser His Val Ala Ser His Gly Phe Ile Leu 
65                  70                  75                  80  
Val Ala Pro Gln Leu Cys Lys Leu Leu Pro Pro Gly Gly Gln Val Glu 
                85                  90                  95      
Val Asp Asp Ala Gly Lys Val Ile Asn Trp Ala Pro Lys Tyr Leu Lys 
            100                 105                 110         
Ser Leu Leu Pro Gly Ser Val Lys Pro Ser Gly Glu Asp Thr Ser Leu 
        115                 120                 125             
Val Gly His Ser Arg Gly Gly Lys Thr Ala Phe Ala Val Ala Leu Gly 
    130                 135                 140                 
His Ala Ser Thr Leu Asp Pro Ser Thr Lys Phe Ser Ala Leu Val Gly 
145                 150                 155                 160 
Ile Asp Pro Val Ala Gly Ser Asn Val Cys Met Arg Thr Gln Pro His 
                165                 170                 175     
Ile Leu Thr Tyr Glu Pro Glu Ser Phe Glu Leu Asp Met Pro Val Ala 
            180                 185                 190         
Val Val Gly Thr Gly Leu Gly Pro Lys Trp Asn Asn Val Met Pro Pro 
        195                 200                 205             
Cys Ala Pro Asp Gly Val Asn His Lys Glu Phe Phe Asn Glu Cys Arg 
    210                 215                 220                 
Pro Thr Arg Ala His Phe Val Ala Ala Asp Tyr Gly His Met Asp Met 
225                 230                 235                 240 
Leu Asp Asp Asp Leu Glu Gly Ala Val Gly Tyr Leu Ala Gly Cys Leu 
                245                 250                 255     
Cys Lys Lys Gly Lys Leu Asp Lys Ser Gly Met Arg Arg Phe Val Gly 
            260                 265                 270         
Gly Ile Val Val Ala Phe Leu Lys Tyr Ser Val Phe Gly Asp Lys Ser 
        275                 280                 285             
Glu Ile Asn Ser Ile Val Lys Asp Pro Ser Leu Ser Pro Ala Arg Ile 
    290                 295                 300                 
Asp Pro Pro Pro Gln Phe His Glu Ala Ser Ser Phe Val 
305                 310                 315         

<210> 8
<211> 306
<212> PRT
<213> Solanum tuberosum


<220> 
<223> CHL31 putative chlorophyllase

<400> 8
Met Val His Gly Lys Ala Ser Ile Glu Arg Asn Ile Lys Met Gly Ala 
1               5                   10                  15      
Ser Ser Ile Phe Glu Ile Gly Asn Glu Thr Ile Asn Thr Ile Asn Val 
            20                  25                  30          
Lys Ser Ser Ser Ser Leu Pro Cys Ser Leu Leu Val Phe Ser Pro Thr 
        35                  40                  45              
Thr Lys Gly Ser Tyr Pro Val Leu Leu Phe Phe His Gly Phe Met Leu 
    50                  55                  60                  
Gln Pro Ser Trp Tyr Lys Ser Leu Leu Gln His Ile Ser Ser His Arg 
65                  70                  75                  80  
Tyr Ile Ile Val Ala Pro Gln Phe Pro Leu Ile Asn Leu Gln Glu Met 
                85                  90                  95      
Lys Asn Val Arg Lys Ile Ala Glu Trp Leu Ile Asn Asn Leu Lys Ser 
            100                 105                 110         
Val Val Pro Glu Lys Val Gln Pro Asp Leu Glu Lys Val Ala Ile Ser 
        115                 120                 125             
Gly His Ser Lys Gly Gly Asn Thr Ala Phe Ala Val Ala Phe Asp Ser 
    130                 135                 140                 
Ser Met Pro Leu Lys Phe Ser Ala Leu Leu Gly Ile Glu Pro Val Ala 
145                 150                 155                 160 
Gly Thr Ser Thr Ser Cys Leu Cys Pro Pro Tyr Val Leu Glu Tyr Ile 
                165                 170                 175     
Pro Arg Ile Phe Asn Gln Ser Ile Pro Val Ala Val Leu Gly Ala Gly 
            180                 185                 190         
Leu Ser Asn Gln Ser Thr Cys Cys Leu Leu Gln Ser Val Ala Pro Asn 
        195                 200                 205             
Gly Val Asn His Ala Glu Phe Phe Asn Glu Ser Lys Pro Pro Cys Tyr 
    210                 215                 220                 
Tyr Phe Met Ala Lys Asp Tyr Gly His Ala Asp Met Leu Glu Ala Glu 
225                 230                 235                 240 
Gly Ile Met Ala Ile Leu Ile Arg Ile Leu Met Lys Ser Gly Lys Gly 
                245                 250                 255     
Ser Lys Asp Ser Met Ile Arg Ala Val Gly Gly Ile Val Val Ala Phe 
            260                 265                 270         
Leu Lys Ala Tyr Leu Glu Gly Gln Ile Asp Asp Leu Asn Asp Ile Val 
        275                 280                 285             
Lys Ser Pro Asn Leu Ala Pro Ile Thr Leu Asp Pro Val Ile Ser Ile 
    290                 295                 300                 
Lys Asp 
305     

<210> 9
<211> 343
<212> PRT
<213> Populus trichocarpa


<220> 
<223> CHL32 putative chlorophyllase

<400> 9
Met Ala Ala Ile Glu Asp Ser Pro Thr Phe Ser Ser Val Val Thr Pro 
1               5                   10                  15      
Ala Ala Phe Glu Ile Gly Ser Leu Pro Thr Thr Glu Ile Pro Val Asp 
            20                  25                  30          
Pro Val Glu Asn Asp Ser Thr Ala Pro Pro Gly Ser Leu Leu Ile Phe 
        35                  40                  45              
Arg Pro Glu Glu Lys Gly Thr Tyr Pro Val Ile Leu Phe His His Gly 
    50                  55                  60                  
Thr Gly Cys Gln Asn Ser Trp Tyr Thr Asp Val Phe Lys Phe Ile Ser 
65                  70                  75                  80  
Ser His Gly Tyr Ile Val Val Ala Pro Gln Leu Tyr Gly Leu Met Pro 
                85                  90                  95      
Pro Ser Gly Gln Asp Glu Leu Asp Leu Ala Ala Glu Val Ala Asn Trp 
            100                 105                 110         
Leu Pro Ser Gly Leu Arg Cys Val Leu Pro Glu Asp Ile Glu Gly Asp 
        115                 120                 125             
Ile His Asn Leu Ala Leu Ala Gly His Ser Arg Gly Gly Tyr Ile Ala 
    130                 135                 140                 
Phe Ala Leu Ala Leu Gly Leu Ala Asp Val Ser Leu Asp Val Asp Phe 
145                 150                 155                 160 
Ser Ala Leu Ile Gly Val Asp Pro Val Ala Gly Thr Ser Lys Thr Asn 
                165                 170                 175     
Gln Met Glu Pro Lys Ile Leu Asn Tyr Glu Ser Cys Ser Phe Asn Phe 
            180                 185                 190         
Ser Ile Pro Val Ala Ile Ile Gly Thr Gly Leu Gly Asn Lys Pro Ala 
        195                 200                 205             
Cys Pro Ile Ile Arg Gln Thr Cys Ala Pro Asp Gly Val Ser His Thr 
    210                 215                 220                 
Glu Ile Phe Asn Glu Cys Lys Pro Pro Cys Ser His Phe Val Thr Thr 
225                 230                 235                 240 
Asp Tyr Gly His Met Asp Val Leu Asp Asp Asp Ile Gly Leu Ile Gly 
                245                 250                 255     
Glu Gly Ala Arg Ala Met Cys Lys Gly Ser Arg Arg Gly Val Ser Arg 
            260                 265                 270         
Asp Pro Met Arg Arg Thr Val Gly Gly Val Ser Val Ala Phe Leu Glu 
        275                 280                 285             
Ala Phe Phe Lys Gly Asn Tyr Thr Asp Tyr Asn Lys Ile Leu Lys Ser 
    290                 295                 300                 
Asn Tyr Phe Ala Pro Thr Thr Leu Asp Pro Val Gln Asn Lys Ser Glu 
305                 310                 315                 320 
Gly Thr Ser Ser Ser Ser Leu Ser Ala Met Ser Met Pro Ala Thr Leu 
                325                 330                 335     
Asp Trp His Ile Asp Glu Leu 
            340             

<210> 10
<211> 318
<212> PRT
<213> Vigna radiata


<220> 
<223> CHL33 putative chlorophyllase

<400> 10
Met Ala Ala Ile Glu Asp Ser Pro Thr Phe Ser Ser Val Val Thr Pro 
1               5                   10                  15      
Ala Ala Phe Glu Ile Gly Ser Leu Pro Thr Thr Glu Ile Pro Val Asp 
            20                  25                  30          
Pro Val Glu Asn Asp Ser Thr Ala Pro Pro Lys Pro Leu Leu Ile Phe 
        35                  40                  45              
Thr Pro Thr Val Pro Gly Ala Tyr Pro Val Ile Leu Phe Cys His Gly 
    50                  55                  60                  
Phe Phe Val Pro Asn Thr Phe Tyr Ser His Leu Leu Thr His Ile Val 
65                  70                  75                  80  
Ser His Gly Phe Ile Leu Val Ala Pro Gln Leu Phe Cys Lys Gly Leu 
                85                  90                  95      
Pro Met Leu Glu Pro Ser Glu Val Lys Phe Ala Gly Lys Val Ala Asp 
            100                 105                 110         
Trp Leu Ala Glu Gly Leu Gln Pro Leu Leu Pro Glu Asn Val Glu Ala 
        115                 120                 125             
Asn Leu Glu Lys Leu Val Val Ser Gly His Ser Lys Gly Gly Lys Thr 
    130                 135                 140                 
Ala Phe Cys Val Ala Leu Gly Tyr Ala Lys Thr Lys Leu Lys Phe Ser 
145                 150                 155                 160 
Ala Leu Val Gly Ile Asp Pro Val Ala Gly Thr Ser Lys Tyr Cys Glu 
                165                 170                 175     
Thr Asn Pro His Ile Leu Lys Gly Val Pro Gly Ser Phe Asn Leu Asn 
            180                 185                 190         
Met Pro Val Ala Val Ile Gly Ser Glu Leu Gly Pro Lys Lys Gly Asn 
        195                 200                 205             
Cys Cys Ser Pro Pro Cys Ala Pro Asp Gly Met Asn His Lys Glu Phe 
    210                 215                 220                 
Phe Lys Glu Cys Lys Pro Pro Ser Ala His Phe Val Val Ala Arg Tyr 
225                 230                 235                 240 
Gly His Met Asp Met Leu Asp Asp Glu Thr Ala Gly Val Ile Gly Thr 
                245                 250                 255     
Leu Leu Ser Lys Cys Ala Cys Lys Asn Gly Ser Gly Pro Arg Asp Leu 
            260                 265                 270         
Met Arg Arg Thr Ile Gly Gly Leu Val Val Ala Phe Leu Arg Ala Gln 
        275                 280                 285             
Leu Asn Asp His Trp Lys Asp Phe Asp Ala Ile Leu Asn Pro Asn Ile 
    290                 295                 300                 
Ala Pro Thr Gln Leu Asp Asn Met Val Tyr Ile Pro Ala Ser 
305                 310                 315             

<210> 11
<211> 236
<212> PRT
<213> Artificial Sequence


<220> 
<223> Green fluorescent protein

<400> 11
Met Thr Ala Leu Thr Glu Gly Ala Lys Leu Phe Glu Lys Glu Ile Pro 
1               5                   10                  15      
Tyr Ile Thr Glu Leu Glu Gly Asp Val Glu Gly Met Lys Phe Ile Ile 
            20                  25                  30          
Lys Gly Glu Gly Thr Gly Asp Ala Thr Thr Gly Thr Ile Lys Ala Lys 
        35                  40                  45              
Tyr Ile Cys Thr Thr Gly Asp Leu Pro Val Pro Trp Ala Thr Leu Val 
    50                  55                  60                  
Ser Thr Leu Ser Tyr Gly Val Gln Cys Phe Ala Lys Tyr Pro Ser His 
65                  70                  75                  80  
Ile Lys Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Thr Gln Glu 
                85                  90                  95      
Arg Thr Ile Ser Phe Glu Gly Asp Gly Val Tyr Lys Thr Arg Ala Met 
            100                 105                 110         
Val Thr Tyr Glu Arg Gly Ser Ile Tyr Asn Arg Val Thr Leu Thr Gly 
        115                 120                 125             
Glu Asn Phe Lys Lys Asp Gly His Ile Leu Arg Lys Asn Val Ala Phe 
    130                 135                 140                 
Gln Cys Pro Pro Ser Ile Leu Tyr Ile Leu Pro Asp Thr Val Asn Asn 
145                 150                 155                 160 
Gly Ile Arg Val Glu Phe Asn Gln Ala Tyr Asp Ile Glu Gly Val Thr 
                165                 170                 175     
Glu Lys Leu Val Thr Lys Cys Ser Gln Met Asn Arg Pro Leu Ala Gly 
            180                 185                 190         
Ser Ala Ala Val His Ile Pro Arg Tyr His His Ile Thr Tyr His Thr 
        195                 200                 205             
Lys Leu Ser Lys Asp Arg Asp Glu Arg Arg Asp His Met Cys Leu Val 
    210                 215                 220                 
Glu Val Val Lys Ala Val Asp Leu Asp Thr Tyr Gln 
225                 230                 235     

<210> 12
<211> 301
<212> PRT
<213> Chlamydomonas reinhardtii


<220> 
<223> P2 pyropheophytinase

<400> 12
Met Ser Asp Asp Tyr Ile Lys Arg Gly Asp Leu Pro Thr Ser Lys Trp 
1               5                   10                  15      
Ser Gly Arg Val Thr Leu Arg Val Asp Ser Ala Met Ala Val Pro Leu 
            20                  25                  30          
Asp Val Val Ile Thr Tyr Pro Ser Ser Gly Ala Ala Ala Tyr Pro Val 
        35                  40                  45              
Leu Val Met Tyr Asn Gly Phe Gln Ala Lys Ala Pro Trp Tyr Arg Gly 
    50                  55                  60                  
Ile Val Asp His Val Ser Ser Trp Gly Tyr Thr Val Val Gln Tyr Thr 
65                  70                  75                  80  
Asn Gly Gly Leu Phe Pro Ile Val Val Asp Arg Val Glu Leu Thr Tyr 
                85                  90                  95      
Leu Glu Pro Leu Leu Thr Trp Leu Glu Thr Gln Ser Ala Asp Ala Lys 
            100                 105                 110         
Ser Pro Leu Tyr Gly Arg Ala Asp Val Ser Arg Leu Gly Thr Met Gly 
        115                 120                 125             
His Ser Arg Gly Gly Lys Leu Ala Ala Leu Gln Phe Ala Gly Arg Thr 
    130                 135                 140                 
Asp Val Ser Gly Cys Val Leu Phe Asp Pro Val Asp Gly Ser Pro Met 
145                 150                 155                 160 
Thr Pro Glu Ser Ala Asp Tyr Pro Ser Ala Thr Lys Ala Leu Ala Ala 
                165                 170                 175     
Ala Gly Arg Ser Ala Gly Leu Val Gly Ala Ala Ile Thr Gly Ser Cys 
            180                 185                 190         
Asn Pro Val Gly Gln Asn Tyr Pro Lys Phe Trp Gly Ala Leu Ala Pro 
        195                 200                 205             
Gly Ser Trp Gln Met Val Leu Ser Gln Ala Gly His Met Gln Phe Ala 
    210                 215                 220                 
Arg Thr Gly Asn Pro Phe Leu Asp Trp Ser Leu Asp Arg Leu Cys Gly 
225                 230                 235                 240 
Arg Gly Thr Met Met Ser Ser Asp Val Ile Thr Tyr Ser Ala Ala Phe 
                245                 250                 255     
Thr Val Ala Trp Phe Glu Gly Ile Phe Arg Pro Ala Gln Ser Gln Met 
            260                 265                 270         
Gly Ile Ser Asn Phe Lys Thr Trp Ala Asn Thr Gln Val Ala Ala Arg 
        275                 280                 285             
Ser Ile Thr Phe Asp Ile Lys Pro Met Gln Ser Pro Gln 
    290                 295                 300     

<210> 13
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> SpeI site and ribosome binding site

<400> 13
actagtagga ggtaactaat g                                              21


<210> 14
<211> 12
<212> DNA
<213> Artificial Sequence


<220> 
<223> stop codon and XhoI site

<400> 14
tgatgactcg ag                                                        12


