               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> IMPROVED GLYCEROL FREE ETHANOL PRODUCTION

<130> 32596-WO-PCT

<160> 53

<170> BiSSAP 1.3.6

<210> 1
<211> 365
<212> PRT
<213> Klebsiella pneumoniae


<220> 
<223> K. pneumoniae glycerol dehydrogenase (Kpne_gldA) amino acid
      sequence

<400> 1
Met Leu Lys Val Ile Gln Ser Pro Ala Lys Tyr Leu Gln Gly Pro Asp 
1               5                   10                  15      
Ala Ala Val Leu Phe Gly Gln Tyr Ala Lys Asn Leu Ala Glu Ser Phe 
            20                  25                  30          
Phe Val Ile Ala Asp Asp Phe Val Met Lys Leu Ala Gly Glu Lys Val 
        35                  40                  45              
Val Asn Gly Leu Gln Ser His Asp Ile Arg Cys His Ala Glu Arg Phe 
    50                  55                  60                  
Asn Gly Glu Cys Ser His Ala Glu Ile Asn Arg Leu Met Ala Ile Leu 
65                  70                  75                  80  
Gln Lys Gln Gly Cys Arg Gly Val Val Gly Ile Gly Gly Gly Lys Thr 
                85                  90                  95      
Leu Asp Thr Ala Lys Ala Ile Gly Tyr Tyr Gln Lys Leu Pro Val Val 
            100                 105                 110         
Val Ile Pro Thr Ile Ala Ser Thr Asp Ala Pro Thr Ser Ala Leu Ser 
        115                 120                 125             
Val Ile Tyr Thr Glu Ala Gly Glu Phe Glu Glu Tyr Leu Ile Tyr Pro 
    130                 135                 140                 
Lys Asn Pro Asp Met Val Val Met Asp Thr Ala Ile Ile Ala Lys Ala 
145                 150                 155                 160 
Pro Val Arg Leu Leu Val Ser Gly Met Gly Asp Ala Leu Ser Thr Trp 
                165                 170                 175     
Phe Glu Ala Lys Ala Cys Tyr Asp Ala Arg Ala Thr Ser Met Ala Gly 
            180                 185                 190         
Gly Gln Ser Thr Glu Ala Ala Leu Ser Leu Ala Arg Leu Cys Tyr Asp 
        195                 200                 205             
Thr Leu Leu Ala Glu Gly Glu Lys Ala Arg Leu Ala Ala Gln Ala Gly 
    210                 215                 220                 
Val Val Thr Glu Ala Leu Glu Arg Ile Ile Glu Ala Asn Thr Tyr Leu 
225                 230                 235                 240 
Ser Gly Ile Gly Phe Glu Ser Ser Gly Leu Ala Ala Ala His Ala Ile 
                245                 250                 255     
His Asn Gly Phe Thr Ile Leu Glu Glu Cys His His Leu Tyr His Gly 
            260                 265                 270         
Glu Lys Val Ala Phe Gly Thr Leu Ala Gln Leu Val Leu Gln Asn Ser 
        275                 280                 285             
Pro Met Asp Glu Ile Glu Thr Val Leu Gly Phe Cys Gln Arg Val Gly 
    290                 295                 300                 
Leu Pro Val Thr Leu Ala Gln Met Gly Val Lys Glu Gly Ile Asp Ala 
305                 310                 315                 320 
Lys Ile Ala Ala Val Ala Lys Ala Thr Cys Ala Glu Gly Glu Thr Ile 
                325                 330                 335     
His Asn Met Pro Phe Ala Val Thr Pro Glu Ser Val His Ala Ala Ile 
            340                 345                 350         
Leu Thr Ala Asp Leu Leu Gly Gln Gln Trp Leu Ala Arg 
        355                 360                 365 

<210> 2
<211> 367
<212> PRT
<213> Enterococcus aerogenes


<220> 
<223> E. aerogenes glycerol dehydrogenase (Eaer_gldA) amino acid
      sequence

<400> 2
Met Asp Arg Ile Ile Gln Ser Pro Gly Lys Tyr Ile Gln Gly Ala Gly 
1               5                   10                  15      
Ala Ile Lys Arg Leu Gly Glu Tyr Leu Lys Pro Leu Ala Glu Arg Trp 
            20                  25                  30          
Leu Ile Ile Gly Asp Lys Phe Val Leu Gly Phe Ala Glu Glu Gln Leu 
        35                  40                  45              
Arg Thr Ser Leu Gly Gly Ala Gly Leu Val Ala Glu Ile Ala Pro Phe 
    50                  55                  60                  
Gly Gly Glu Cys Ser Gln Asn Glu Ile Asn Arg Leu Arg Asp Ile Ala 
65                  70                  75                  80  
Ser Ser Ala Gln Cys His Ala Val Leu Gly Ile Gly Gly Gly Lys Thr 
                85                  90                  95      
Leu Asp Thr Ala Lys Ala Leu Ala His Tyr Met His Leu Pro Val Val 
            100                 105                 110         
Val Ala Pro Thr Ile Ala Ser Thr Asp Ala Pro Cys Ser Ala Leu Ser 
        115                 120                 125             
Val Ile Tyr Thr Asp Asp Gly Glu Phe Glu Ser Tyr Leu Met Leu Pro 
    130                 135                 140                 
His Asn Pro Asn Met Val Val Val Asp Thr Gln Ile Val Ala Ala Ala 
145                 150                 155                 160 
Pro Ala Arg Leu Leu Ala Ala Gly Ile Gly Asp Ala Leu Ala Thr Trp 
                165                 170                 175     
Phe Glu Ala Arg Ala Cys Ser Arg Ser Gly Ala Thr Thr Met Ala Gly 
            180                 185                 190         
Gly Lys Cys Thr Gln Ala Ala Leu Ala Leu Ala Glu Leu Cys Tyr Asn 
        195                 200                 205             
Thr Leu Val Glu Glu Gly Glu Lys Ala Met Leu Ala Ala Glu Gln His 
    210                 215                 220                 
Val Val Thr Pro Ala Leu Glu Arg Val Ile Glu Ala Asn Thr Tyr Leu 
225                 230                 235                 240 
Ser Gly Val Gly Phe Glu Ser Gly Gly Leu Ala Ala Ala His Ala Ile 
                245                 250                 255     
His Asn Gly Leu Thr Ala Ile Pro Asp Ala His His Phe Tyr His Gly 
            260                 265                 270         
Glu Lys Val Ala Phe Gly Thr Leu Thr Gln Leu Val Leu Glu Asn Ala 
        275                 280                 285             
Pro Val Glu Glu Ile Glu Thr Ala Ala Ala Leu Cys His Ser Val Gly 
    290                 295                 300                 
Leu Pro Ile Thr Leu Ala Gln Leu Asp Ile Lys Gly Asp Ile Pro Ala 
305                 310                 315                 320 
Lys Met Arg Thr Val Ala Glu Ala Ala Cys Ala Glu Gly Glu Thr Ile 
                325                 330                 335     
His Asn Met Pro Gly Gly Ala Cys Ala Asp Gln Val Tyr Ala Ala Leu 
            340                 345                 350         
Leu Val Ala Asp Gln Tyr Gly Gln Arg Phe Leu Gln Glu Trp Glu 
        355                 360                 365         

<210> 3
<211> 364
<212> PRT
<213> Yersinia aldovae


<220> 
<223> Y. aldovae glycerol dehydrogenase (Eaer_gldA) amino acid sequence

<400> 3
Met Leu Lys Val Ile Gln Ser Pro Ser Lys Tyr Ile Gln Gly Ala Asn 
1               5                   10                  15      
Ala Leu Gln Ser Ile Gly Glu Phe Ala Lys Leu Leu Ala Asn Asn Tyr 
            20                  25                  30          
Phe Ile Ile Ala Asp Asp Phe Val Met Lys Leu Thr Ala Asp Thr Val 
        35                  40                  45              
Gly Thr Ser Leu Gln Thr Cys Glu Leu Lys Ser His Phe Ser Arg Phe 
    50                  55                  60                  
Asn Gly Glu Cys Ser Arg Gln Glu Ile Glu Arg Leu Thr Val Glu Leu 
65                  70                  75                  80  
Lys Lys Tyr Gly Cys Asn Gly Val Ile Gly Ile Gly Gly Gly Lys Thr 
                85                  90                  95      
Leu Asp Thr Ala Lys Ala Ile Ala His Tyr Gln His Ile Pro Val Val 
            100                 105                 110         
Val Val Pro Thr Ile Ala Ser Thr Asp Ala Pro Thr Ser Ala Leu Ser 
        115                 120                 125             
Val Ile Tyr Thr Glu Gln Gly Glu Phe Ala Glu Tyr Leu Ile Tyr Pro 
    130                 135                 140                 
Lys Asn Pro Asp Ile Val Leu Met Asp Thr Thr Ile Ile Ala Lys Ala 
145                 150                 155                 160 
Pro Val Arg Leu Leu Val Ala Gly Met Gly Asp Ala Leu Ser Thr Tyr 
                165                 170                 175     
Phe Glu Ala Gln Ala Cys Phe Asp Ala Lys Ala Ile Ser Met Ala Gly 
            180                 185                 190         
Gly Ala Ser Thr Leu Ala Ala Ile Thr Leu Ala Arg Leu Cys Tyr Glu 
        195                 200                 205             
Thr Leu Leu Ala Glu Gly Tyr Lys Ala Lys Leu Ala Val Glu Ala Gly 
    210                 215                 220                 
Val Val Thr Glu Ala Val Glu Arg Ile Ile Glu Ala Asn Thr Tyr Leu 
225                 230                 235                 240 
Ser Gly Ile Gly Phe Glu Ser Ser Gly Leu Ala Ala Ala His Ala Ile 
                245                 250                 255     
His Asn Gly Phe Thr Val Leu Glu Glu Cys His His Leu Tyr His Gly 
            260                 265                 270         
Glu Lys Val Ala Phe Gly Thr Leu Thr Gln Leu Val Leu Gln Asn Ser 
        275                 280                 285             
Ser Met Glu Glu Ile Glu Thr Val Leu Ser Phe Cys Gln Gln Leu Gly 
    290                 295                 300                 
Leu Pro Ile Thr Leu Ala Glu Met Gly Val Thr Gln Asp Leu Glu Cys 
305                 310                 315                 320 
Lys Ile Arg Ala Val Ala Gln Ala Ser Cys Ala Glu Gly Glu Thr Ile 
                325                 330                 335     
His Asn Met Pro Phe Lys Val Thr Ala Asp Ser Val Tyr Ala Ala Ile 
            340                 345                 350         
Ile Val Ala Asp Arg Leu Gly Gln Ala Phe Leu Asn 
        355                 360                 

<210> 4
<211> 572
<212> PRT
<213> Klebsiella pneumoniae


<220> 
<223> K. pneumoniae dihydroxyacetone kinase (Kpne_dhaK) amino acid
      sequence

<400> 4
Met Thr Thr Lys Gln Phe Gln Phe Asp Ser Asp Pro Leu Asn Ser Ala 
1               5                   10                  15      
Leu Ala Ala Thr Ala Glu Ala Ser Gly Leu Ala Tyr Leu Pro Lys Ser 
            20                  25                  30          
Lys Val Ile Tyr Tyr Pro Leu Thr Asn Asp Lys Val Thr Leu Ile Ser 
        35                  40                  45              
Gly Gly Gly Ala Gly His Glu Pro Ala Gln Thr Gly Phe Val Gly Pro 
    50                  55                  60                  
Gly Leu Leu Asp Ala Ala Val Ser Gly Gln Ile Phe Ala Ser Pro Ser 
65                  70                  75                  80  
Thr Lys Gln Ile Ile Ala Gly Val Asn Ala Val Lys Ser Gln Arg Gly 
                85                  90                  95      
Ser Ile Ile Ile Val Met Asn Tyr Thr Gly Asp Val Ile His Phe Gly 
            100                 105                 110         
Met Ala Ala Glu Gln Leu Arg Ser Arg Tyr Asp Tyr His Ala Glu Leu 
        115                 120                 125             
Val Ser Ile Gly Asp Asp Ile Ser Val Asn Lys Lys Ala Gly Arg Arg 
    130                 135                 140                 
Gly Leu Ala Gly Thr Val Leu Val His Lys Ile Ala Gly His Leu Ala 
145                 150                 155                 160 
Arg Asp Gly Trp Asp Val Gly Val Leu Ala Glu Ala Leu Arg Thr Thr 
                165                 170                 175     
Ala Ala Asn Leu Ala Thr Val Ala Ala Ser Leu Glu His Cys Thr Val 
            180                 185                 190         
Pro Gly Arg Lys Phe Glu Thr Glu Leu Ala Ala Asp Glu Met Glu Ile 
        195                 200                 205             
Gly Met Gly Ile His Asn Glu Pro Gly Val Lys Thr Ile Lys Ile Gly 
    210                 215                 220                 
Lys Val Glu Ser Leu Leu Asp Glu Leu Val Asp Lys Phe Glu Pro Ser 
225                 230                 235                 240 
Lys Gln Asp Phe Val Pro Phe Asn Lys Gly Asp Glu Val Val Leu Leu 
                245                 250                 255     
Val Asn Ser Leu Gly Gly Val Ser Ser Leu Glu Leu His Ala Ile Ala 
            260                 265                 270         
Asn Ile Ala Gln Thr Lys Phe Glu Lys Val Leu Gly Val Lys Thr Val 
        275                 280                 285             
Arg Leu Ile Val Gly Asn Phe Met Ala Ala Phe Asn Gly Pro Gly Phe 
    290                 295                 300                 
Ser Leu Thr Leu Leu Asn Val Thr Thr Thr Ala Lys Lys Gly Asn Phe 
305                 310                 315                 320 
Asp Val Leu Gly Ala Leu Asp Ala Pro Val Ser Thr Ala Ala Trp Pro 
                325                 330                 335     
Ser Leu Gln Gln Lys Asp Lys Pro Ala Asn Gly Gly Val Gln Glu Glu 
            340                 345                 350         
Lys Glu Thr Asp Ser Asp Lys Pro Ala Glu Pro Thr Gly Ile Lys Ala 
        355                 360                 365             
Asp Gly Lys Leu Phe Lys Ala Met Ile Glu Ser Ala Val Asp Asp Leu 
    370                 375                 380                 
Lys Lys Glu Glu Pro Gln Ile Thr Lys Tyr Asp Thr Ile Ala Gly Asp 
385                 390                 395                 400 
Gly Asp Cys Gly Glu Thr Leu Leu Ala Gly Gly Asp Gly Ile Leu Asp 
                405                 410                 415     
Ala Ile Lys Asn Lys Lys Ile Asp Leu Asp Asp Ala Ala Gly Val Ala 
            420                 425                 430         
Asp Ile Ser His Ile Val Glu Asn Ser Met Gly Gly Thr Ser Gly Gly 
        435                 440                 445             
Leu Tyr Ser Ile Phe Phe Ser Gly Leu Val Val Gly Ile Lys Glu Thr 
    450                 455                 460                 
Lys Ala Lys Glu Leu Ser Val Asp Val Phe Ala Lys Ala Cys Glu Thr 
465                 470                 475                 480 
Ala Leu Glu Thr Leu Ser Lys Tyr Thr Gln Ala Arg Val Gly Asp Arg 
                485                 490                 495     
Thr Leu Met Asp Ala Leu Val Pro Phe Val Glu Thr Leu Ser Lys Thr 
            500                 505                 510         
Lys Asp Phe Ala Lys Ala Val Glu Ala Ala Arg Lys Gly Ala Asp Glu 
        515                 520                 525             
Thr Ser Lys Leu Pro Ala Asn Phe Gly Arg Ala Ser Tyr Val Asn Glu 
    530                 535                 540                 
Glu Gly Leu Glu Asn Ile Pro Asp Pro Gly Ala Leu Gly Leu Ala Val 
545                 550                 555                 560 
Ile Phe Glu Gly Leu Leu Lys Ala Trp Glu Lys Lys 
                565                 570         

<210> 5
<211> 580
<212> PRT
<213> Yarrowia lipolytica


<220> 
<223> Y. lipolytica dihydroxyacetone kinase (Ylip_DAK1) amino acid
      sequence

<400> 5
Met Asp Lys His Phe Ile Asn Asp Pro Glu Val Leu Val Leu Asp Gly 
1               5                   10                  15      
Leu Lys Ser Leu Ala Asp Met Asn Lys Thr Leu Thr Val His Glu Glu 
            20                  25                  30          
Gly Lys Phe Ile Tyr Phe His Asp Tyr Asn Lys Lys Asn Val Ser Val 
        35                  40                  45              
Ile Ser Gly Gly Gly Ala Gly His Glu Pro Thr His Ser Ser Phe Val 
    50                  55                  60                  
Gly Lys Gly Met Leu Thr Ala Ala Val Ser Gly Ser Ile Phe Ala Ser 
65                  70                  75                  80  
Pro Ser Ser Lys Gln Ile Tyr Thr Gly Ile Lys Gln Val Glu Ser Glu 
                85                  90                  95      
Ala Gly Thr Leu Val Ile Cys Lys Asn Tyr Thr Gly Asp Ile Leu His 
            100                 105                 110         
Phe Gly Met Ala Leu Glu Lys Gln Arg Thr Ala Gly Lys Lys Ala Glu 
        115                 120                 125             
Leu Ile Ala Val Ala Asp Asp Val Ser Val Gly Arg Lys Lys Ser Gly 
    130                 135                 140                 
Lys Val Gly Arg Arg Gly Leu Ser Gly Thr Val Leu Val His Lys Ile 
145                 150                 155                 160 
Ala Gly Ala Ala Ala Ala Arg Gly Leu Pro Leu Glu Ala Val Thr Thr 
                165                 170                 175     
Ile Ala Lys Ala Ala Ile Asp Asn Leu Val Ser Ile Gly Ala Ser Leu 
            180                 185                 190         
Ala His Val His Val Pro Gly His Glu Pro Ile Ala Lys Glu Asp Glu 
        195                 200                 205             
Met Lys His Asp Glu Met Glu Leu Gly Met Gly Ile His Asn Glu Pro 
    210                 215                 220                 
Gly Cys Lys Arg Ile Ser Pro Ile Pro Ser Ile Asp Asp Leu Ile Ala 
225                 230                 235                 240 
Gln Met Leu Lys Gln Met Leu Asp Gln Ser Asp Lys Asp Arg Ala Tyr 
                245                 250                 255     
Val Lys Ile Glu Gly Asp Asp Glu Val Val Leu Leu Met Asn Asn Leu 
            260                 265                 270         
Gly Gly Leu Ser Met Leu Glu Phe Ser Ala Ile Ser His Lys Val Lys 
        275                 280                 285             
Glu Ala Leu Ala Lys Glu Tyr Lys Ile Asn Pro Val Arg Ile Phe Ala 
    290                 295                 300                 
Gly Pro Phe Thr Thr Ser Leu Asn Gly Leu Gly Phe Gly Ile Thr Leu 
305                 310                 315                 320 
Leu Arg Thr Thr Asp Arg Val Lys Val Glu Gly Glu Glu Tyr Ser Leu 
                325                 330                 335     
Val Asp Leu Ile Asp Gln Pro Val Glu Ala Ile Gly Trp Pro Leu Cys 
            340                 345                 350         
Gln Pro Ser Asp Leu Lys Ser Lys Asn Lys Ile Gly Asn Val Ser Ile 
        355                 360                 365             
Glu Glu Gly Gln Lys Asp Val Lys Ser Pro Val Thr Val Asp Lys Glu 
    370                 375                 380                 
Lys Val Arg Gln Ala Ile Val Asn Ser Met Glu Asn Leu Ile Lys Ala 
385                 390                 395                 400 
Glu Pro Lys Ile Thr Lys Phe Asp Thr Met Ala Gly Asp Gly Asp Cys 
                405                 410                 415     
Gly Thr Thr Leu Lys Arg Gly Ala Glu Gly Val Leu Lys Phe Val Lys 
            420                 425                 430         
Ser Asp Lys Phe Ser Asp Asp Pro Ile Arg Ile Val Arg Asp Ile Ala 
        435                 440                 445             
Asp Val Ile Glu Asp Asn Met Asp Gly Thr Ser Gly Ala Leu Tyr Ala 
    450                 455                 460                 
Ile Phe Phe His Gly Phe Ala Lys Gly Met Lys Asp Thr Leu Glu Lys 
465                 470                 475                 480 
Ser Lys Asp Ile Ser Ser Lys Thr Trp Ala Ala Gly Leu Lys Val Ala 
                485                 490                 495     
Leu Asp Thr Leu Phe Lys Tyr Thr Pro Ala Arg Pro Gly Asp Ser Thr 
            500                 505                 510         
Met Cys Asp Ala Leu Val Pro Phe Val Glu Thr Phe Val Lys Thr Asn 
        515                 520                 525             
Asp Leu Asn Ala Ala Val Glu Glu Ala Arg Lys Gly Ala Asp Ala Thr 
    530                 535                 540                 
Ala Asp Met Gln Ala Lys Leu Gly Arg Ala Val Tyr Val Gly Asp Asp 
545                 550                 555                 560 
Val Lys Val Pro Asp Ala Gly Ala Leu Gly Val Val Ala Ile Val Glu 
                565                 570                 575     
Gly Phe Thr Lys 
            580 

<210> 6
<211> 580
<212> PRT
<213> Schizosaccharomyces pombe


<220> 
<223> S. pombe dihydroxyacetone kinase (Spom_DAK1) amino acid sequence

<400> 6
Met Asp Lys His Phe Ile Asn Asp Pro Glu Val Leu Val Leu Asp Gly 
1               5                   10                  15      
Leu Lys Ser Leu Ala Asp Met Asn Lys Thr Leu Thr Val His Glu Glu 
            20                  25                  30          
Gly Lys Phe Ile Tyr Phe His Asp Tyr Asn Lys Lys Asn Val Ser Val 
        35                  40                  45              
Ile Ser Gly Gly Gly Ala Gly His Glu Pro Thr His Ser Ser Phe Val 
    50                  55                  60                  
Gly Lys Gly Met Leu Thr Ala Ala Val Ser Gly Ser Ile Phe Ala Ser 
65                  70                  75                  80  
Pro Ser Ser Lys Gln Ile Tyr Thr Gly Ile Lys Gln Val Glu Ser Glu 
                85                  90                  95      
Ala Gly Thr Leu Val Ile Cys Lys Asn Tyr Thr Gly Asp Ile Leu His 
            100                 105                 110         
Phe Gly Met Ala Leu Glu Lys Gln Arg Thr Ala Gly Lys Lys Ala Glu 
        115                 120                 125             
Leu Ile Ala Val Ala Asp Asp Val Ser Val Gly Arg Lys Lys Ser Gly 
    130                 135                 140                 
Lys Val Gly Arg Arg Gly Leu Ser Gly Thr Val Leu Val His Lys Ile 
145                 150                 155                 160 
Ala Gly Ala Ala Ala Ala Arg Gly Leu Pro Leu Glu Ala Val Thr Thr 
                165                 170                 175     
Ile Ala Lys Ala Ala Ile Asp Asn Leu Val Ser Ile Gly Ala Ser Leu 
            180                 185                 190         
Ala His Val His Val Pro Gly His Glu Pro Ile Ala Lys Glu Asp Glu 
        195                 200                 205             
Met Lys His Asp Glu Met Glu Leu Gly Met Gly Ile His Asn Glu Pro 
    210                 215                 220                 
Gly Cys Lys Arg Ile Ser Pro Ile Pro Ser Ile Asp Asp Leu Ile Ala 
225                 230                 235                 240 
Gln Met Leu Lys Gln Met Leu Asp Gln Ser Asp Lys Asp Arg Ala Tyr 
                245                 250                 255     
Val Lys Ile Glu Gly Asp Asp Glu Val Val Leu Leu Met Asn Asn Leu 
            260                 265                 270         
Gly Gly Leu Ser Met Leu Glu Phe Ser Ala Ile Ser His Lys Val Lys 
        275                 280                 285             
Glu Ala Leu Ala Lys Glu Tyr Lys Ile Asn Pro Val Arg Ile Phe Ala 
    290                 295                 300                 
Gly Pro Phe Thr Thr Ser Leu Asn Gly Leu Gly Phe Gly Ile Thr Leu 
305                 310                 315                 320 
Leu Arg Thr Thr Asp Arg Val Lys Val Glu Gly Glu Glu Tyr Ser Leu 
                325                 330                 335     
Val Asp Leu Ile Asp Gln Pro Val Glu Ala Ile Gly Trp Pro Leu Cys 
            340                 345                 350         
Gln Pro Ser Asp Leu Lys Ser Lys Asn Lys Ile Gly Asn Val Ser Ile 
        355                 360                 365             
Glu Glu Gly Gln Lys Asp Val Lys Ser Pro Val Thr Val Asp Lys Glu 
    370                 375                 380                 
Lys Val Arg Gln Ala Ile Val Asn Ser Met Glu Asn Leu Ile Lys Ala 
385                 390                 395                 400 
Glu Pro Lys Ile Thr Lys Phe Asp Thr Met Ala Gly Asp Gly Asp Cys 
                405                 410                 415     
Gly Thr Thr Leu Lys Arg Gly Ala Glu Gly Val Leu Lys Phe Val Lys 
            420                 425                 430         
Ser Asp Lys Phe Ser Asp Asp Pro Ile Arg Ile Val Arg Asp Ile Ala 
        435                 440                 445             
Asp Val Ile Glu Asp Asn Met Asp Gly Thr Ser Gly Ala Leu Tyr Ala 
    450                 455                 460                 
Ile Phe Phe His Gly Phe Ala Lys Gly Met Lys Asp Thr Leu Glu Lys 
465                 470                 475                 480 
Ser Lys Asp Ile Ser Ser Lys Thr Trp Ala Ala Gly Leu Lys Val Ala 
                485                 490                 495     
Leu Asp Thr Leu Phe Lys Tyr Thr Pro Ala Arg Pro Gly Asp Ser Thr 
            500                 505                 510         
Met Cys Asp Ala Leu Val Pro Phe Val Glu Thr Phe Val Lys Thr Asn 
        515                 520                 525             
Asp Leu Asn Ala Ala Val Glu Glu Ala Arg Lys Gly Ala Asp Ala Thr 
    530                 535                 540                 
Ala Asp Met Gln Ala Lys Leu Gly Arg Ala Val Tyr Val Gly Asp Asp 
545                 550                 555                 560 
Val Lys Val Pro Asp Ala Gly Ala Leu Gly Val Val Ala Ile Val Glu 
                565                 570                 575     
Gly Phe Thr Lys 
            580 

<210> 7
<211> 291
<212> PRT
<213> Danio rerio


<220> 
<223> D. rerio aquaporin 9 (Drer_T3) amino acid sequence

<400> 7
Met Glu Tyr Leu Glu Asn Ile Arg Asn Leu Arg Gly Arg Cys Val Leu 
1               5                   10                  15      
Arg Arg Asp Ile Ile Arg Glu Phe Leu Ala Glu Leu Leu Gly Thr Phe 
            20                  25                  30          
Val Leu Ile Leu Phe Gly Cys Gly Ser Val Ala Gln Thr Val Leu Ser 
        35                  40                  45              
Arg Glu Ala Lys Gly Gln Leu Leu Thr Ile His Phe Gly Phe Thr Leu 
    50                  55                  60                  
Gly Val Met Leu Ala Val Tyr Met Ala Gly Gly Val Ser Gly Gly His 
65                  70                  75                  80  
Val Asn Pro Ala Val Ser Leu Ala Met Val Val Leu Arg Lys Leu Pro 
                85                  90                  95      
Leu Lys Lys Phe Pro Val Tyr Val Leu Ala Gln Phe Leu Gly Ala Phe 
            100                 105                 110         
Phe Gly Ser Cys Ala Val Tyr Cys Leu Tyr Tyr Asp Ala Phe Thr Glu 
        115                 120                 125             
Phe Ala Asn Gly Glu Leu Ala Val Thr Gly Pro Asn Val Thr Ala Gly 
    130                 135                 140                 
Ile Phe Ala Ser Tyr Pro Arg Glu Gly Leu Ser Leu Leu Asn Gly Phe 
145                 150                 155                 160 
Ile Asp Gln Val Ile Gly Ala Gly Ala Leu Val Leu Cys Ile Leu Ala 
                165                 170                 175     
Val Val Asp Lys Lys Asn Ile Gly Ala Pro Lys Gly Met Glu Pro Leu 
            180                 185                 190         
Leu Val Gly Leu Ser Ile Leu Ala Ile Gly Val Ser Met Ala Leu Asn 
        195                 200                 205             
Cys Gly Tyr Pro Ile Asn Pro Ala Arg Asp Leu Gly Pro Arg Leu Phe 
    210                 215                 220                 
Thr Ala Ile Ala Gly Trp Gly Leu Thr Val Phe Ser Ala Gly Asn Gly 
225                 230                 235                 240 
Trp Trp Trp Val Pro Val Val Gly Pro Met Val Gly Gly Val Val Gly 
                245                 250                 255     
Ala Ala Ile Tyr Phe Leu Met Ile Glu Met His His Pro Glu Asn Asp 
            260                 265                 270         
Lys Asn Leu Glu Asp Asp Asn Ser Leu Lys Asp Lys Tyr Glu Leu Asn 
        275                 280                 285             
Thr Val Asn 
    290     

<210> 8
<211> 592
<212> PRT
<213> Zygosaccharomyces rouxii


<220> 
<223> Z. rouxii ZYRO0E01210p (Zrou_T5) amino acid sequence

<400> 8
Met Gly Lys Arg Thr Gln Gly Phe Met Asp Tyr Val Phe Ser Arg Thr 
1               5                   10                  15      
Ser Thr Ala Gly Leu Lys Gly Ala Arg Leu Arg Tyr Thr Ala Ala Ala 
            20                  25                  30          
Val Ala Val Ile Gly Phe Ala Leu Phe Gly Tyr Asp Gln Gly Leu Met 
        35                  40                  45              
Ser Gly Leu Ile Thr Gly Asp Gln Phe Asn Lys Glu Phe Pro Pro Thr 
    50                  55                  60                  
Lys Ser Asn Gly Asp Asn Asp Arg Tyr Ala Ser Val Ile Gln Gly Ala 
65                  70                  75                  80  
Val Thr Ala Cys Tyr Glu Ile Gly Cys Phe Phe Gly Ser Leu Phe Val 
                85                  90                  95      
Leu Phe Phe Gly Asp Ala Ile Gly Arg Lys Pro Leu Ile Ile Phe Gly 
            100                 105                 110         
Ala Ile Ile Val Ile Ile Gly Thr Val Ile Ser Thr Ala Pro Phe His 
        115                 120                 125             
His Ala Trp Gly Leu Gly Gln Phe Val Val Gly Arg Val Ile Thr Gly 
    130                 135                 140                 
Val Gly Thr Gly Phe Asn Thr Ser Thr Ile Pro Val Trp Gln Ser Glu 
145                 150                 155                 160 
Met Thr Lys Pro Asn Ile Arg Gly Ala Met Ile Asn Leu Asp Gly Ser 
                165                 170                 175     
Val Ile Ala Phe Gly Thr Met Ile Ala Tyr Trp Leu Asp Phe Gly Phe 
            180                 185                 190         
Ser Phe Ile Asn Ser Ser Val Gln Trp Arg Phe Pro Val Ser Val Gln 
        195                 200                 205             
Ile Ile Phe Ala Leu Val Leu Leu Phe Gly Ile Val Arg Met Pro Glu 
    210                 215                 220                 
Ser Pro Arg Trp Leu Met Ala Lys Lys Arg Pro Ala Glu Ala Arg Tyr 
225                 230                 235                 240 
Val Leu Ala Cys Leu Asn Asp Leu Pro Glu Asn Asp Asp Ala Ile Leu 
                245                 250                 255     
Ala Glu Met Thr Ser Leu His Glu Ala Val Asn Arg Ser Ser Asn Gln 
            260                 265                 270         
Lys Ser Gln Met Lys Ser Leu Phe Ser Met Gly Lys Gln Gln Asn Phe 
        275                 280                 285             
Ser Arg Ala Leu Ile Ala Ser Ser Thr Gln Phe Phe Gln Gln Phe Thr 
    290                 295                 300                 
Gly Cys Asn Ala Ala Ile Tyr Tyr Ser Thr Val Leu Phe Gln Thr Thr 
305                 310                 315                 320 
Val Gln Leu Asp Arg Leu Leu Ala Met Ile Leu Gly Gly Val Phe Ala 
                325                 330                 335     
Thr Val Tyr Thr Leu Ser Thr Leu Pro Ser Phe Tyr Leu Val Glu Lys 
            340                 345                 350         
Val Gly Arg Arg Lys Met Phe Phe Phe Gly Ala Leu Gly Gln Gly Ile 
        355                 360                 365             
Ser Phe Ile Ile Thr Phe Ala Cys Leu Val Asn Pro Thr Lys Gln Asn 
    370                 375                 380                 
Ala Lys Gly Ala Ala Val Gly Leu Tyr Leu Phe Ile Ile Cys Phe Gly 
385                 390                 395                 400 
Leu Ala Ile Leu Glu Leu Pro Trp Ile Tyr Pro Pro Glu Ile Ala Ser 
                405                 410                 415     
Met Arg Val Arg Ala Ala Thr Asn Ala Met Ser Thr Cys Thr Asn Trp 
            420                 425                 430         
Val Thr Asn Phe Ala Val Val Met Phe Thr Pro Val Phe Ile Gln Thr 
        435                 440                 445             
Ser Gln Trp Gly Cys Tyr Leu Phe Phe Ala Val Met Asn Phe Ile Tyr 
    450                 455                 460                 
Leu Pro Val Ile Phe Phe Phe Tyr Pro Glu Thr Ala Gly Arg Ser Leu 
465                 470                 475                 480 
Glu Glu Ile Asp Ile Ile Phe Ala Lys Ala His Val Asp Gly Thr Leu 
                485                 490                 495     
Pro Trp Met Val Ala His Arg Leu Pro Lys Leu Ser Met Thr Glu Val 
            500                 505                 510         
Glu Asp Tyr Ser Gln Ser Leu Gly Leu His Asp Asp Glu Asn Glu Lys 
        515                 520                 525             
Glu Glu Tyr Asp Glu Lys Glu Ala Glu Ala Asn Ala Ala Leu Phe Gln 
    530                 535                 540                 
Val Glu Thr Ser Ser Lys Ser Pro Ser Ser Asn Arg Lys Asp Asp Asp 
545                 550                 555                 560 
Ala Pro Ile Glu His Asn Glu Val Gln Glu Ser Asn Asp Asn Ser Ser 
                565                 570                 575     
Asn Ser Ser Asn Val Glu Ala Pro Ile Pro Val His His Asn Asp Pro 
            580                 585                 590         



<210> 9
<211> 97
<212> PRT
<213> Escherichia coli


<220> 
<223> groES from E. coli

<400> 9
Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 
1               5                   10                  15      
Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 
            20                  25                  30          
Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 
        35                  40                  45              
Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 
    50                  55                  60                  
Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 
65                  70                  75                  80  
Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 
                85                  90                  95      
Ala 
    

<210> 10
<211> 548
<212> PRT
<213> Escherichia coli


<220> 
<223> groEL from E. coli

<400> 10
Met Ala Ala Lys Asp Val Lys Phe Gly Asn Asp Ala Arg Val Lys Met 
1               5                   10                  15      
Leu Arg Gly Val Asn Val Leu Ala Asp Ala Val Lys Val Thr Leu Gly 
            20                  25                  30          
Pro Lys Gly Arg Asn Val Val Leu Asp Lys Ser Phe Gly Ala Pro Thr 
        35                  40                  45              
Ile Thr Lys Asp Gly Val Ser Val Ala Arg Glu Ile Glu Leu Glu Asp 
    50                  55                  60                  
Lys Phe Glu Asn Met Gly Ala Gln Met Val Lys Glu Val Ala Ser Lys 
65                  70                  75                  80  
Ala Asn Asp Ala Ala Gly Asp Gly Thr Thr Thr Ala Thr Val Leu Ala 
                85                  90                  95      
Gln Ala Ile Ile Thr Glu Gly Leu Lys Ala Val Ala Ala Gly Met Asn 
            100                 105                 110         
Pro Met Asp Leu Lys Arg Gly Ile Asp Lys Ala Val Thr Ala Ala Val 
        115                 120                 125             
Glu Glu Leu Lys Ala Leu Ser Val Pro Cys Ser Asp Ser Lys Ala Ile 
    130                 135                 140                 
Ala Gln Val Gly Thr Ile Ser Ala Asn Ser Asp Glu Thr Val Gly Lys 
145                 150                 155                 160 
Leu Ile Ala Glu Ala Met Asp Lys Val Gly Lys Glu Gly Val Ile Thr 
                165                 170                 175     
Val Glu Asp Gly Thr Gly Leu Gln Asp Glu Leu Asp Val Val Glu Gly 
            180                 185                 190         
Met Gln Phe Asp Arg Gly Tyr Leu Ser Pro Tyr Phe Ile Asn Lys Pro 
        195                 200                 205             
Glu Thr Gly Ala Val Glu Leu Glu Ser Pro Phe Ile Leu Leu Ala Asp 
    210                 215                 220                 
Lys Lys Ile Ser Asn Ile Arg Glu Met Leu Pro Val Leu Glu Ala Val 
225                 230                 235                 240 
Ala Lys Ala Gly Lys Pro Leu Leu Ile Ile Ala Glu Asp Val Glu Gly 
                245                 250                 255     
Glu Ala Leu Ala Thr Leu Val Val Asn Thr Met Arg Gly Ile Val Lys 
            260                 265                 270         
Val Ala Ala Val Lys Ala Pro Gly Phe Gly Asp Arg Arg Lys Ala Met 
        275                 280                 285             
Leu Gln Asp Ile Ala Thr Leu Thr Gly Gly Thr Val Ile Ser Glu Glu 
    290                 295                 300                 
Ile Gly Met Glu Leu Glu Lys Ala Thr Leu Glu Asp Leu Gly Gln Ala 
305                 310                 315                 320 
Lys Arg Val Val Ile Asn Lys Asp Thr Thr Thr Ile Ile Asp Gly Val 
                325                 330                 335     
Gly Glu Glu Ala Ala Ile Gln Gly Arg Val Ala Gln Ile Arg Gln Gln 
            340                 345                 350         
Ile Glu Glu Ala Thr Ser Asp Tyr Asp Arg Glu Lys Leu Gln Glu Arg 
        355                 360                 365             
Val Ala Lys Leu Ala Gly Gly Val Ala Val Ile Lys Val Gly Ala Ala 
    370                 375                 380                 
Thr Glu Val Glu Met Lys Glu Lys Lys Ala Arg Val Glu Asp Ala Leu 
385                 390                 395                 400 
His Ala Thr Arg Ala Ala Val Glu Glu Gly Val Val Ala Gly Gly Gly 
                405                 410                 415     
Val Ala Leu Ile Arg Val Ala Ser Lys Leu Ala Asp Leu Arg Gly Gln 
            420                 425                 430         
Asn Glu Asp Gln Asn Val Gly Ile Lys Val Ala Leu Arg Ala Met Glu 
        435                 440                 445             
Ala Pro Leu Arg Gln Ile Val Leu Asn Cys Gly Glu Glu Pro Ser Val 
    450                 455                 460                 
Val Ala Asn Thr Val Lys Gly Gly Asp Gly Asn Tyr Gly Tyr Asn Ala 
465                 470                 475                 480 
Ala Thr Glu Glu Tyr Gly Asn Met Ile Asp Met Gly Ile Leu Asp Pro 
                485                 490                 495     
Thr Lys Val Thr Arg Ser Ala Leu Gln Tyr Ala Ala Ser Val Ala Gly 
            500                 505                 510         
Leu Met Ile Thr Thr Glu Cys Met Val Thr Asp Leu Pro Lys Asn Asp 
        515                 520                 525             
Ala Ala Asp Leu Gly Ala Ala Gly Gly Met Gly Gly Met Gly Gly Met 
    530                 535                 540                 
Gly Gly Met Met 
545             

<210> 11
<211> 459
<212> PRT
<213> Thiobacillus denitrificans


<220> 
<223> RubisCO cbbM gene from Thiobacillus denitrificans

<400> 11
Met Asp Gln Ser Ala Arg Tyr Ala Asp Leu Ser Leu Lys Glu Glu Asp 
1               5                   10                  15      
Leu Ile Lys Gly Gly Arg His Ile Leu Val Ala Tyr Lys Met Lys Pro 
            20                  25                  30          
Lys Ser Gly Tyr Gly Tyr Leu Glu Ala Ala Ala His Phe Ala Ala Glu 
        35                  40                  45              
Ser Ser Thr Gly Thr Asn Val Glu Val Ser Thr Thr Asp Asp Phe Thr 
    50                  55                  60                  
Lys Gly Val Asp Ala Leu Val Tyr Tyr Ile Asp Glu Ala Ser Glu Asp 
65                  70                  75                  80  
Met Arg Ile Ala Tyr Pro Leu Glu Leu Phe Asp Arg Asn Val Thr Asp 
                85                  90                  95      
Gly Arg Phe Met Leu Val Ser Phe Leu Thr Leu Ala Ile Gly Asn Asn 
            100                 105                 110         
Gln Gly Met Gly Asp Ile Glu His Ala Lys Met Ile Asp Phe Tyr Val 
        115                 120                 125             
Pro Glu Arg Cys Ile Gln Met Phe Asp Gly Pro Ala Thr Asp Ile Ser 
    130                 135                 140                 
Asn Leu Trp Arg Ile Leu Gly Arg Pro Val Val Asn Gly Gly Tyr Ile 
145                 150                 155                 160 
Ala Gly Thr Ile Ile Lys Pro Lys Leu Gly Leu Arg Pro Glu Pro Phe 
                165                 170                 175     
Ala Lys Ala Ala Tyr Gln Phe Trp Leu Gly Gly Asp Phe Ile Lys Asn 
            180                 185                 190         
Asp Glu Pro Gln Gly Asn Gln Val Phe Cys Pro Leu Lys Lys Val Leu 
        195                 200                 205             
Pro Leu Val Tyr Asp Ala Met Lys Arg Ala Gln Asp Asp Thr Gly Gln 
    210                 215                 220                 
Ala Lys Leu Phe Ser Met Asn Ile Thr Ala Asp Asp His Tyr Glu Met 
225                 230                 235                 240 
Cys Ala Arg Ala Asp Tyr Ala Leu Glu Val Phe Gly Pro Asp Ala Asp 
                245                 250                 255     
Lys Leu Ala Phe Leu Val Asp Gly Tyr Val Gly Gly Pro Gly Met Val 
            260                 265                 270         
Thr Thr Ala Arg Arg Gln Tyr Pro Gly Gln Tyr Leu His Tyr His Arg 
        275                 280                 285             
Ala Gly His Gly Ala Val Thr Ser Pro Ser Ala Lys Arg Gly Tyr Thr 
    290                 295                 300                 
Ala Phe Val Leu Ala Lys Met Ser Arg Leu Gln Gly Ala Ser Gly Ile 
305                 310                 315                 320 
His Val Gly Thr Met Gly Tyr Gly Lys Met Glu Gly Glu Gly Asp Asp 
                325                 330                 335     
Lys Ile Ile Ala Tyr Met Ile Glu Arg Asp Glu Cys Gln Gly Pro Val 
            340                 345                 350         
Tyr Phe Gln Lys Trp Tyr Gly Met Lys Pro Thr Thr Pro Ile Ile Ser 
        355                 360                 365             
Gly Gly Met Asn Ala Leu Arg Leu Pro Gly Phe Phe Glu Asn Leu Gly 
    370                 375                 380                 
His Gly Asn Val Ile Asn Thr Ala Gly Gly Gly Ser Tyr Gly His Ile 
385                 390                 395                 400 
Asp Ser Pro Ala Ala Gly Ala Ile Ser Leu Arg Gln Ser Tyr Glu Cys 
                405                 410                 415     
Trp Lys Gln Gly Ala Asp Pro Ile Glu Phe Ala Lys Glu His Lys Glu 
            420                 425                 430         
Phe Ala Arg Ala Phe Glu Ser Phe Pro Lys Asp Ala Asp Lys Leu Phe 
        435                 440                 445             
Pro Gly Trp Arg Glu Lys Leu Gly Val His Ser 
    450                 455                 

<210> 12
<211> 352
<212> PRT
<213> Spinacia oleracea


<220> 
<223> PRK spinacia

<400> 12
Met Ser Gln Gln Gln Thr Ile Val Ile Gly Leu Ala Ala Asp Ser Gly 
1               5                   10                  15      
Cys Gly Lys Ser Thr Phe Met Arg Arg Leu Thr Ser Val Phe Gly Gly 
            20                  25                  30          
Ala Ala Glu Pro Pro Lys Gly Gly Asn Pro Asp Ser Asn Thr Leu Ile 
        35                  40                  45              
Ser Asp Thr Thr Thr Val Ile Cys Leu Asp Asp Phe His Ser Leu Asp 
    50                  55                  60                  
Arg Asn Gly Arg Lys Val Glu Lys Val Thr Ala Leu Asp Pro Lys Ala 
65                  70                  75                  80  
Asn Asp Phe Asp Leu Met Tyr Glu Gln Val Lys Ala Leu Lys Glu Gly 
                85                  90                  95      
Lys Ala Val Asp Lys Pro Ile Tyr Asn His Val Ser Gly Leu Leu Asp 
            100                 105                 110         
Pro Pro Glu Leu Ile Gln Pro Pro Lys Ile Leu Val Ile Glu Gly Leu 
        115                 120                 125             
His Pro Met Tyr Asp Ala Arg Val Arg Glu Leu Leu Asp Phe Ser Ile 
    130                 135                 140                 
Tyr Leu Asp Ile Ser Asn Glu Val Lys Phe Ala Trp Lys Ile Gln Arg 
145                 150                 155                 160 
Asp Met Lys Glu Arg Gly His Ser Leu Glu Ser Ile Lys Ala Ser Ile 
                165                 170                 175     
Glu Ser Arg Lys Pro Asp Phe Asp Ala Tyr Ile Asp Pro Gln Lys Gln 
            180                 185                 190         
His Ala Asp Val Val Ile Glu Val Leu Pro Thr Glu Leu Ile Pro Asp 
        195                 200                 205             
Asp Asp Glu Gly Lys Val Leu Arg Val Arg Met Ile Gln Lys Glu Gly 
    210                 215                 220                 
Val Lys Phe Phe Asn Pro Val Tyr Leu Phe Asp Glu Gly Ser Thr Ile 
225                 230                 235                 240 
Ser Trp Ile Pro Cys Gly Arg Lys Leu Thr Cys Ser Tyr Pro Gly Ile 
                245                 250                 255     
Lys Phe Ser Tyr Gly Pro Asp Thr Phe Tyr Gly Asn Glu Val Thr Val 
            260                 265                 270         
Val Glu Met Asp Gly Met Phe Asp Arg Leu Asp Glu Leu Ile Tyr Val 
        275                 280                 285             
Glu Ser His Leu Ser Asn Leu Ser Thr Lys Phe Tyr Gly Glu Val Thr 
    290                 295                 300                 
Gln Gln Met Leu Lys His Gln Asn Phe Pro Gly Ser Asn Asn Gly Thr 
305                 310                 315                 320 
Gly Phe Phe Gln Thr Ile Ile Gly Leu Lys Ile Arg Asp Leu Phe Glu 
                325                 330                 335     
Gln Leu Val Ala Ser Arg Ser Thr Ala Thr Ala Thr Ala Ala Lys Ala 
            340                 345                 350         



<210> 13
<211> 367
<212> PRT
<213> Escherichia coli


<220> 
<223> E. coli glycerol dehydrogenase (Ec_gldA) amino acid sequence

<400> 13
Met Asp Arg Ile Ile Gln Ser Pro Gly Lys Tyr Ile Gln Gly Ala Asp 
1               5                   10                  15      
Val Ile Asn Arg Leu Gly Glu Tyr Leu Lys Pro Leu Ala Glu Arg Trp 
            20                  25                  30          
Leu Val Val Gly Asp Lys Phe Val Leu Gly Phe Ala Gln Ser Thr Val 
        35                  40                  45              
Glu Lys Ser Phe Lys Asp Ala Gly Leu Val Val Glu Ile Ala Pro Phe 
    50                  55                  60                  
Gly Gly Glu Cys Ser Gln Asn Glu Ile Asp Arg Leu Arg Gly Ile Ala 
65                  70                  75                  80  
Glu Thr Ala Gln Cys Gly Ala Ile Leu Gly Ile Gly Gly Gly Lys Thr 
                85                  90                  95      
Leu Asp Thr Ala Lys Ala Leu Ala His Phe Met Gly Val Pro Val Ala 
            100                 105                 110         
Ile Ala Pro Thr Ile Ala Ser Thr Asp Ala Pro Cys Ser Ala Leu Ser 
        115                 120                 125             
Val Ile Tyr Thr Asp Glu Gly Glu Phe Asp Arg Tyr Leu Leu Leu Pro 
    130                 135                 140                 
Asn Asn Pro Asn Met Val Ile Val Asp Thr Lys Ile Val Ala Gly Ala 
145                 150                 155                 160 
Pro Ala Arg Leu Leu Ala Ala Gly Ile Gly Asp Ala Leu Ala Thr Trp 
                165                 170                 175     
Phe Glu Ala Arg Ala Cys Ser Arg Ser Gly Ala Thr Thr Met Ala Gly 
            180                 185                 190         
Gly Lys Cys Thr Gln Ala Ala Leu Ala Leu Ala Glu Leu Cys Tyr Asn 
        195                 200                 205             
Thr Leu Leu Glu Glu Gly Glu Lys Ala Met Leu Ala Ala Glu Gln His 
    210                 215                 220                 
Val Val Thr Pro Ala Leu Glu Arg Val Ile Glu Ala Asn Thr Tyr Leu 
225                 230                 235                 240 
Ser Gly Val Gly Phe Glu Ser Gly Gly Leu Ala Ala Ala His Ala Val 
                245                 250                 255     
His Asn Gly Leu Thr Ala Ile Pro Asp Ala His His Tyr Tyr His Gly 
            260                 265                 270         
Glu Lys Val Ala Phe Gly Thr Leu Thr Gln Leu Val Leu Glu Asn Ala 
        275                 280                 285             
Pro Val Glu Glu Ile Glu Thr Val Ala Ala Leu Ser His Ala Val Gly 
    290                 295                 300                 
Leu Pro Ile Thr Leu Ala Gln Leu Asp Ile Lys Glu Asp Val Pro Ala 
305                 310                 315                 320 
Lys Met Arg Ile Val Ala Glu Ala Ala Cys Ala Glu Gly Glu Thr Ile 
                325                 330                 335     
His Asn Met Pro Gly Gly Ala Thr Pro Asp Gln Val Tyr Ala Ala Leu 
            340                 345                 350         
Leu Val Ala Asp Gln Tyr Gly Gln Arg Phe Leu Gln Glu Trp Glu 
        355                 360                 365         

<210> 14
<211> 584
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> S. cerevisiae dihydroxyacetone kinase (S. cerevisiae DAK1) amino
      acid sequence

<400> 14
Met Ser Ala Lys Ser Phe Glu Val Thr Asp Pro Val Asn Ser Ser Leu 
1               5                   10                  15      
Lys Gly Phe Ala Leu Ala Asn Pro Ser Ile Thr Leu Val Pro Glu Glu 
            20                  25                  30          
Lys Ile Leu Phe Arg Lys Thr Asp Ser Asp Lys Ile Ala Leu Ile Ser 
        35                  40                  45              
Gly Gly Gly Ser Gly His Glu Pro Thr His Ala Gly Phe Ile Gly Lys 
    50                  55                  60                  
Gly Met Leu Ser Gly Ala Val Val Gly Glu Ile Phe Ala Ser Pro Ser 
65                  70                  75                  80  
Thr Lys Gln Ile Leu Asn Ala Ile Arg Leu Val Asn Glu Asn Ala Ser 
                85                  90                  95      
Gly Val Leu Leu Ile Val Lys Asn Tyr Thr Gly Asp Val Leu His Phe 
            100                 105                 110         
Gly Leu Ser Ala Glu Arg Ala Arg Ala Leu Gly Ile Asn Cys Arg Val 
        115                 120                 125             
Ala Val Ile Gly Asp Asp Val Ala Val Gly Arg Glu Lys Gly Gly Met 
    130                 135                 140                 
Val Gly Arg Arg Ala Leu Ala Gly Thr Val Leu Val His Lys Ile Val 
145                 150                 155                 160 
Gly Ala Phe Ala Glu Glu Tyr Ser Ser Lys Tyr Gly Leu Asp Gly Thr 
                165                 170                 175     
Ala Lys Val Ala Lys Ile Ile Asn Asp Asn Leu Val Thr Ile Gly Ser 
            180                 185                 190         
Ser Leu Asp His Cys Lys Val Pro Gly Arg Lys Phe Glu Ser Glu Leu 
        195                 200                 205             
Asn Glu Lys Gln Met Glu Leu Gly Met Gly Ile His Asn Glu Pro Gly 
    210                 215                 220                 
Val Lys Val Leu Asp Pro Ile Pro Ser Thr Glu Asp Leu Ile Ser Lys 
225                 230                 235                 240 
Tyr Met Leu Pro Lys Leu Leu Asp Pro Asn Asp Lys Asp Arg Ala Phe 
                245                 250                 255     
Val Lys Phe Asp Glu Asp Asp Glu Val Val Leu Leu Val Asn Asn Leu 
            260                 265                 270         
Gly Gly Val Ser Asn Phe Val Ile Ser Ser Ile Thr Ser Lys Thr Thr 
        275                 280                 285             
Asp Phe Leu Lys Glu Asn Tyr Asn Ile Thr Pro Val Gln Thr Ile Ala 
    290                 295                 300                 
Gly Thr Leu Met Thr Ser Phe Asn Gly Asn Gly Phe Ser Ile Thr Leu 
305                 310                 315                 320 
Leu Asn Ala Thr Lys Ala Thr Lys Ala Leu Gln Ser Asp Phe Glu Glu 
                325                 330                 335     
Ile Lys Ser Val Leu Asp Leu Leu Asn Ala Phe Thr Asn Ala Pro Gly 
            340                 345                 350         
Trp Pro Ile Ala Asp Phe Glu Lys Thr Ser Ala Pro Ser Val Asn Asp 
        355                 360                 365             
Asp Leu Leu His Asn Glu Val Thr Ala Lys Ala Val Gly Thr Tyr Asp 
    370                 375                 380                 
Phe Asp Lys Phe Ala Glu Trp Met Lys Ser Gly Ala Glu Gln Val Ile 
385                 390                 395                 400 
Lys Ser Glu Pro His Ile Thr Glu Leu Asp Asn Gln Val Gly Asp Gly 
                405                 410                 415     
Asp Cys Gly Tyr Thr Leu Val Ala Gly Val Lys Gly Ile Thr Glu Asn 
            420                 425                 430         
Leu Asp Lys Leu Ser Lys Asp Ser Leu Ser Gln Ala Val Ala Gln Ile 
        435                 440                 445             
Ser Asp Phe Ile Glu Gly Ser Met Gly Gly Thr Ser Gly Gly Leu Tyr 
    450                 455                 460                 
Ser Ile Leu Leu Ser Gly Phe Ser His Gly Leu Ile Gln Val Cys Lys 
465                 470                 475                 480 
Ser Lys Asp Glu Pro Val Thr Lys Glu Ile Val Ala Lys Ser Leu Gly 
                485                 490                 495     
Ile Ala Leu Asp Thr Leu Tyr Lys Tyr Thr Lys Ala Arg Lys Gly Ser 
            500                 505                 510         
Ser Thr Met Ile Asp Ala Leu Glu Pro Phe Val Lys Glu Phe Thr Ala 
        515                 520                 525             
Ser Lys Asp Phe Asn Lys Ala Val Lys Ala Ala Glu Glu Gly Ala Lys 
    530                 535                 540                 
Ser Thr Ala Thr Phe Glu Ala Lys Phe Gly Arg Ala Ser Tyr Val Gly 
545                 550                 555                 560 
Asp Ser Ser Gln Val Glu Asp Pro Gly Ala Val Gly Leu Cys Glu Phe 
                565                 570                 575     
Leu Lys Gly Val Gln Ser Ala Leu 
            580                 

<210> 15
<211> 12
<212> PRT
<213> Spinacia oleracea


<220> 
<223> PRK motif

<400> 15
Asn Asn Asn Ala Thr Thr Gly Thr Thr Asn Asn Asn 
1               5                   10          

<210> 16
<211> 8
<212> PRT
<213> Spinacia oleracea


<220> 
<223> PRK motif

<400> 16
Thr Cys Gly Thr Thr Tyr Ala Gly 
1               5               

<210> 17
<211> 561
<212> PRT
<213> Punctularia strigosozonata


<220> 
<223> Punctularia strigosozonata glucoamylase

<400> 17
Ser Ser Lys Ser Val Ser Ala Tyr Ile Ser Ser Glu Ser Pro Ile Ala 
1               5                   10                  15      
His Ser Lys Leu Leu Asp Asn Ile Gly Pro Asp Gly Ala Lys Ala Pro 
            20                  25                  30          
Gly Ala Phe Pro Gly Val Val Val Ala Ser Pro Ser Thr Asp Asn Pro 
        35                  40                  45              
Asn Tyr Tyr Tyr Ser Trp Ile Arg Asp Ser Ser Leu Val Phe Lys Thr 
    50                  55                  60                  
Leu Ile Asp Asp Tyr Val Asn Gly Lys Asn Thr Ser Lys Ser Leu Arg 
65                  70                  75                  80  
Ser Leu Ile Asp Asp Phe Val Thr Ala Ser Ser Val Phe Gln Gln Thr 
                85                  90                  95      
Pro Asn Pro Ser Gly Asn Val Ser Thr Gly Gly Leu Gly Glu Pro Lys 
            100                 105                 110         
Phe Tyr Val Asn Glu Thr Ala Phe Leu Asp Ser Trp Gly Arg Pro Gln 
        115                 120                 125             
Arg Asp Gly Pro Ala Leu Arg Ser Thr Ala Leu Ile Thr Tyr Ala Asn 
    130                 135                 140                 
Tyr Leu Leu Asp Asn Asp Asn Thr Thr Trp Val Lys Asp Thr Leu Trp 
145                 150                 155                 160 
Pro Ile Ile Glu Leu Asp Val Asn Tyr Val Ser Asp Phe Trp Asn Tyr 
                165                 170                 175     
Thr Thr Phe Asp Leu Trp Glu Glu Val Ala Ser Ser Ser Phe Phe Thr 
            180                 185                 190         
Thr Ala Val Gln His Arg Ala Leu Arg Gln Ala Ser Lys Leu Ala Lys 
        195                 200                 205             
Thr Leu Asp Lys Thr Asp Asn Ile Asp Ser Trp Asn Thr Gln Ala Asp 
    210                 215                 220                 
Asn Val Leu Cys Phe Leu Gln Ser Tyr Trp Asn Gly Ser Ala Ile Ile 
225                 230                 235                 240 
Ala Asn Thr Gly Gly Gly Arg Ser Gly Ile Asp Ala Asn Thr Val Leu 
                245                 250                 255     
Ala Ser Ile His Thr Phe Asp Ser Ser Ala Gly Cys Asp Ala Thr Thr 
            260                 265                 270         
Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr Val 
        275                 280                 285             
Asp Ala Phe Arg Ser Ile Tyr Glu Ile Asn Ser Gly Ile Asp Pro Asn 
    290                 295                 300                 
Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Val Phe Tyr Asp Gly 
305                 310                 315                 320 
Asn Pro Trp Tyr Leu Ala Thr Ala Ala Val Ala Glu Gln Leu Tyr Asp 
                325                 330                 335     
Ala Leu Tyr Val Trp Asn Thr Thr Gly Ser Leu Glu Ile Thr Asp Ile 
            340                 345                 350         
Ser Leu Pro Phe Phe Gln Gln Phe Asp Ser Asp Val Lys Thr Gly Thr 
        355                 360                 365             
Tyr Ser Asp Asp Asp Thr Phe Asp Ser Leu Ile Ser Ser Ile Gln Ser 
    370                 375                 380                 
Phe Ala Asp Gly Phe Leu Glu Ile His Ala Lys Tyr Thr Pro Asp Asp 
385                 390                 395                 400 
Gly Ala Leu Ser Glu Glu Phe Ser Lys Thr Asp Gly Ser Gln Thr Ser 
                405                 410                 415     
Ala Ala Asp Leu Thr Trp Ser Tyr Ala Ala Ala Leu Thr Ala Phe Asp 
            420                 425                 430         
Ala Arg Ser Arg Asp Ala Ala Val Lys Trp Gly Ala Lys Gly Leu Gln 
        435                 440                 445             
Val Pro Asp Gly Thr Cys Lys Thr Asn Glu Gly Gly Asp Asp Gly Leu 
    450                 455                 460                 
Gly Val Pro Val Thr Phe Leu Val Lys Asp Ala Glu Thr Val Glu Gly 
465                 470                 475                 480 
Gln Ser Val Tyr Ile Thr Gly Ser Ile Ala Thr Leu Lys Ser Trp Ser 
                485                 490                 495     
Pro Asp Asp Ala Leu Leu Met Ser Pro Ser Asp Tyr Pro Thr Trp Thr 
            500                 505                 510         
Leu Thr Val Asn Leu Ser Ala Ser Glu Ser Val Gln Tyr Lys Tyr Ile 
        515                 520                 525             
Lys Lys Asp Thr Ala Gly Thr Val Ile Trp Glu Ser Asp Pro Asn Asn 
    530                 535                 540                 
Ser Leu Leu Val Pro Ser Gly Gly Ser Val Thr Thr Asp Asp Thr Trp 
545                 550                 555                 560 
Arg 
    

<210> 18
<211> 578
<212> PRT
<213> Punctularia strigosozonata


<220> 
<223> Punctularia strigosozonata glucoamylase with native signal
      sequence FGA09

<400> 18
Met Leu Ser Ser Leu Ile Val Ser Gly Leu Leu Ala Ser Gly Val Cys 
1               5                   10                  15      
Ala Ser Ser Lys Ser Val Ser Ala Tyr Ile Ser Ser Glu Ser Pro Ile 
            20                  25                  30          
Ala His Ser Lys Leu Leu Asp Asn Ile Gly Pro Asp Gly Ala Lys Ala 
        35                  40                  45              
Pro Gly Ala Phe Pro Gly Val Val Val Ala Ser Pro Ser Thr Asp Asn 
    50                  55                  60                  
Pro Asn Tyr Tyr Tyr Ser Trp Ile Arg Asp Ser Ser Leu Val Phe Lys 
65                  70                  75                  80  
Thr Leu Ile Asp Asp Tyr Val Asn Gly Lys Asn Thr Ser Lys Ser Leu 
                85                  90                  95      
Arg Ser Leu Ile Asp Asp Phe Val Thr Ala Ser Ser Val Phe Gln Gln 
            100                 105                 110         
Thr Pro Asn Pro Ser Gly Asn Val Ser Thr Gly Gly Leu Gly Glu Pro 
        115                 120                 125             
Lys Phe Tyr Val Asn Glu Thr Ala Phe Leu Asp Ser Trp Gly Arg Pro 
    130                 135                 140                 
Gln Arg Asp Gly Pro Ala Leu Arg Ser Thr Ala Leu Ile Thr Tyr Ala 
145                 150                 155                 160 
Asn Tyr Leu Leu Asp Asn Asp Asn Thr Thr Trp Val Lys Asp Thr Leu 
                165                 170                 175     
Trp Pro Ile Ile Glu Leu Asp Val Asn Tyr Val Ser Asp Phe Trp Asn 
            180                 185                 190         
Tyr Thr Thr Phe Asp Leu Trp Glu Glu Val Ala Ser Ser Ser Phe Phe 
        195                 200                 205             
Thr Thr Ala Val Gln His Arg Ala Leu Arg Gln Ala Ser Lys Leu Ala 
    210                 215                 220                 
Lys Thr Leu Asp Lys Thr Asp Asn Ile Asp Ser Trp Asn Thr Gln Ala 
225                 230                 235                 240 
Asp Asn Val Leu Cys Phe Leu Gln Ser Tyr Trp Asn Gly Ser Ala Ile 
                245                 250                 255     
Ile Ala Asn Thr Gly Gly Gly Arg Ser Gly Ile Asp Ala Asn Thr Val 
            260                 265                 270         
Leu Ala Ser Ile His Thr Phe Asp Ser Ser Ala Gly Cys Asp Ala Thr 
        275                 280                 285             
Thr Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys Val Tyr 
    290                 295                 300                 
Val Asp Ala Phe Arg Ser Ile Tyr Glu Ile Asn Ser Gly Ile Asp Pro 
305                 310                 315                 320 
Asn Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Val Phe Tyr Asp 
                325                 330                 335     
Gly Asn Pro Trp Tyr Leu Ala Thr Ala Ala Val Ala Glu Gln Leu Tyr 
            340                 345                 350         
Asp Ala Leu Tyr Val Trp Asn Thr Thr Gly Ser Leu Glu Ile Thr Asp 
        355                 360                 365             
Ile Ser Leu Pro Phe Phe Gln Gln Phe Asp Ser Asp Val Lys Thr Gly 
    370                 375                 380                 
Thr Tyr Ser Asp Asp Asp Thr Phe Asp Ser Leu Ile Ser Ser Ile Gln 
385                 390                 395                 400 
Ser Phe Ala Asp Gly Phe Leu Glu Ile His Ala Lys Tyr Thr Pro Asp 
                405                 410                 415     
Asp Gly Ala Leu Ser Glu Glu Phe Ser Lys Thr Asp Gly Ser Gln Thr 
            420                 425                 430         
Ser Ala Ala Asp Leu Thr Trp Ser Tyr Ala Ala Ala Leu Thr Ala Phe 
        435                 440                 445             
Asp Ala Arg Ser Arg Asp Ala Ala Val Lys Trp Gly Ala Lys Gly Leu 
    450                 455                 460                 
Gln Val Pro Asp Gly Thr Cys Lys Thr Asn Glu Gly Gly Asp Asp Gly 
465                 470                 475                 480 
Leu Gly Val Pro Val Thr Phe Leu Val Lys Asp Ala Glu Thr Val Glu 
                485                 490                 495     
Gly Gln Ser Val Tyr Ile Thr Gly Ser Ile Ala Thr Leu Lys Ser Trp 
            500                 505                 510         
Ser Pro Asp Asp Ala Leu Leu Met Ser Pro Ser Asp Tyr Pro Thr Trp 
        515                 520                 525             
Thr Leu Thr Val Asn Leu Ser Ala Ser Glu Ser Val Gln Tyr Lys Tyr 
    530                 535                 540                 
Ile Lys Lys Asp Thr Ala Gly Thr Val Ile Trp Glu Ser Asp Pro Asn 
545                 550                 555                 560 
Asn Ser Leu Leu Val Pro Ser Gly Gly Ser Val Thr Thr Asp Asp Thr 
                565                 570                 575     
Trp Arg 
        

<210> 19
<211> 580
<212> PRT
<213> Artificial


<220> 
<223> Punctularia strigosozonata glucoamylase with Sc alpha mating
      factor signal sequence

<400> 19
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      
Ala Leu Ala Ser Ser Lys Ser Val Ser Ala Tyr Ile Ser Ser Glu Ser 
            20                  25                  30          
Pro Ile Ala His Ser Lys Leu Leu Asp Asn Ile Gly Pro Asp Gly Ala 
        35                  40                  45              
Lys Ala Pro Gly Ala Phe Pro Gly Val Val Val Ala Ser Pro Ser Thr 
    50                  55                  60                  
Asp Asn Pro Asn Tyr Tyr Tyr Ser Trp Ile Arg Asp Ser Ser Leu Val 
65                  70                  75                  80  
Phe Lys Thr Leu Ile Asp Asp Tyr Val Asn Gly Lys Asn Thr Ser Lys 
                85                  90                  95      
Ser Leu Arg Ser Leu Ile Asp Asp Phe Val Thr Ala Ser Ser Val Phe 
            100                 105                 110         
Gln Gln Thr Pro Asn Pro Ser Gly Asn Val Ser Thr Gly Gly Leu Gly 
        115                 120                 125             
Glu Pro Lys Phe Tyr Val Asn Glu Thr Ala Phe Leu Asp Ser Trp Gly 
    130                 135                 140                 
Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ser Thr Ala Leu Ile Thr 
145                 150                 155                 160 
Tyr Ala Asn Tyr Leu Leu Asp Asn Asp Asn Thr Thr Trp Val Lys Asp 
                165                 170                 175     
Thr Leu Trp Pro Ile Ile Glu Leu Asp Val Asn Tyr Val Ser Asp Phe 
            180                 185                 190         
Trp Asn Tyr Thr Thr Phe Asp Leu Trp Glu Glu Val Ala Ser Ser Ser 
        195                 200                 205             
Phe Phe Thr Thr Ala Val Gln His Arg Ala Leu Arg Gln Ala Ser Lys 
    210                 215                 220                 
Leu Ala Lys Thr Leu Asp Lys Thr Asp Asn Ile Asp Ser Trp Asn Thr 
225                 230                 235                 240 
Gln Ala Asp Asn Val Leu Cys Phe Leu Gln Ser Tyr Trp Asn Gly Ser 
                245                 250                 255     
Ala Ile Ile Ala Asn Thr Gly Gly Gly Arg Ser Gly Ile Asp Ala Asn 
            260                 265                 270         
Thr Val Leu Ala Ser Ile His Thr Phe Asp Ser Ser Ala Gly Cys Asp 
        275                 280                 285             
Ala Thr Thr Phe Gln Pro Cys Ser Asp Lys Ala Leu Ser Asn Leu Lys 
    290                 295                 300                 
Val Tyr Val Asp Ala Phe Arg Ser Ile Tyr Glu Ile Asn Ser Gly Ile 
305                 310                 315                 320 
Asp Pro Asn Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Val Phe 
                325                 330                 335     
Tyr Asp Gly Asn Pro Trp Tyr Leu Ala Thr Ala Ala Val Ala Glu Gln 
            340                 345                 350         
Leu Tyr Asp Ala Leu Tyr Val Trp Asn Thr Thr Gly Ser Leu Glu Ile 
        355                 360                 365             
Thr Asp Ile Ser Leu Pro Phe Phe Gln Gln Phe Asp Ser Asp Val Lys 
    370                 375                 380                 
Thr Gly Thr Tyr Ser Asp Asp Asp Thr Phe Asp Ser Leu Ile Ser Ser 
385                 390                 395                 400 
Ile Gln Ser Phe Ala Asp Gly Phe Leu Glu Ile His Ala Lys Tyr Thr 
                405                 410                 415     
Pro Asp Asp Gly Ala Leu Ser Glu Glu Phe Ser Lys Thr Asp Gly Ser 
            420                 425                 430         
Gln Thr Ser Ala Ala Asp Leu Thr Trp Ser Tyr Ala Ala Ala Leu Thr 
        435                 440                 445             
Ala Phe Asp Ala Arg Ser Arg Asp Ala Ala Val Lys Trp Gly Ala Lys 
    450                 455                 460                 
Gly Leu Gln Val Pro Asp Gly Thr Cys Lys Thr Asn Glu Gly Gly Asp 
465                 470                 475                 480 
Asp Gly Leu Gly Val Pro Val Thr Phe Leu Val Lys Asp Ala Glu Thr 
                485                 490                 495     
Val Glu Gly Gln Ser Val Tyr Ile Thr Gly Ser Ile Ala Thr Leu Lys 
            500                 505                 510         
Ser Trp Ser Pro Asp Asp Ala Leu Leu Met Ser Pro Ser Asp Tyr Pro 
        515                 520                 525             
Thr Trp Thr Leu Thr Val Asn Leu Ser Ala Ser Glu Ser Val Gln Tyr 
    530                 535                 540                 
Lys Tyr Ile Lys Lys Asp Thr Ala Gly Thr Val Ile Trp Glu Ser Asp 
545                 550                 555                 560 
Pro Asn Asn Ser Leu Leu Val Pro Ser Gly Gly Ser Val Thr Thr Asp 
                565                 570                 575     
Asp Thr Trp Arg 
            580 

<210> 20
<211> 619
<212> PRT
<213> Corynascus sepedonium


<220> 
<223> Corynascus sepedonium glucoamylase (mature)

<400> 20
Arg Pro Gly Ala Ile Pro Arg Ser Gln Arg Gly Gly Ala Ile Ser Lys 
1               5                   10                  15      
Arg Ala Val Asp Ser Tyr Ile Glu Thr Glu Thr Pro Ile Ala Trp Glu 
            20                  25                  30          
Lys Leu Leu Cys Asn Ile Gly Pro Asp Gly Cys Ala Val Ser Gly Ala 
        35                  40                  45              
Ala Ala Gly Ala Val Ile Ala Ser Pro Ser Lys Ser Asp Pro Asp Tyr 
    50                  55                  60                  
Trp Tyr Thr Trp Thr Arg Asp Ala Gly Leu Val Leu Thr Gly Ile Val 
65                  70                  75                  80  
Asp Ser Leu Ala His Asn Tyr Ser Ala Ser Leu Gln Thr Asn Ile Gln 
                85                  90                  95      
Asn Tyr Ile Ile Ala Gln Ala Lys Leu Gln Gly Val Gly Asn Pro Ser 
            100                 105                 110         
Gly Gly Leu Ser Asp Gly Ala Gly Leu Gly Glu Pro Lys Phe Met Val 
        115                 120                 125             
Asp Leu Thr Glu Phe Thr Gly Asp Trp Gly Arg Pro Gln Arg Asp Gly 
    130                 135                 140                 
Pro Pro Leu Arg Ala Ile Ala Leu Ile Arg Tyr Ala Lys Trp Leu Val 
145                 150                 155                 160 
Ala Asn Gly Tyr Lys Asp Thr Ala Asn Glu Leu Val Trp Pro Val Ile 
                165                 170                 175     
Gln Asn Asp Leu Ala Tyr Ala Ala Gln Tyr Trp Asn Glu Thr Gly Phe 
            180                 185                 190         
Asp Leu Trp Glu Glu Val Pro Gly Ser Ser Phe Phe Thr Ile Ala Ser 
        195                 200                 205             
Thr His Arg Ala Leu Val Glu Gly Ala Ala Leu Ala Ala Gln Leu Asp 
    210                 215                 220                 
Thr Glu Cys Arg Ala Cys Ile Thr Val Ala Pro Gln Val Leu Cys Phe 
225                 230                 235                 240 
Leu Gln Thr Phe Trp Asn Pro Ser Gly Gly Tyr Val Val Ser Asn Ile 
                245                 250                 255     
Asn Gly Gly Glu Gly Arg Ser Gly Lys Asp Leu Asn Ser Ile Leu Ala 
            260                 265                 270         
Ser Ala His Thr Phe Asp Pro Ala Ile Gly Cys Asp Ser Val Thr Phe 
        275                 280                 285             
Gln Pro Cys Ser Asp Lys Ala Leu Ala Asn His Lys Ala Tyr Val Asp 
    290                 295                 300                 
Ser Phe Arg Glu Ile Tyr Gly Ile Asn Ser Gly Ile Ala Lys Gly Lys 
305                 310                 315                 320 
Ala Val Ala Val Gly Arg Tyr Ser Glu Asp Val Tyr Tyr Asn Gly Asn 
                325                 330                 335     
Pro Trp Tyr Leu Ala Asn Phe Gly Ala Ala Glu Gln Leu Tyr Asp Ala 
            340                 345                 350         
Ile Tyr Val Trp Lys Glu Gln Gly Ser Ile Glu Val Thr Asp Leu Ser 
        355                 360                 365             
Leu Pro Phe Phe Gln Asp Leu Leu Ser Asp Ile Ser Thr Gly Thr Tyr 
    370                 375                 380                 
Asp Ser Ser Ser Ser Thr Tyr Gln Glu Ile Leu Asp Ala Val Ser Ala 
385                 390                 395                 400 
Tyr Ala Asp Gly Phe Ile Asp Val Ala Ala Gln Tyr Thr Pro Ser Asp 
                405                 410                 415     
Gly Ser Leu Ala Glu Gln Phe Glu Arg Asp Ser Gly Asn Pro Ile Ser 
            420                 425                 430         
Ala Ala Asp Leu Thr Trp Ser Tyr Ser Ala Phe Leu Ser Ala Thr Asp 
        435                 440                 445             
Arg Arg Ala Gly Ile Val Pro Ala Gly Trp Ser Ala Glu Asn Gly Lys 
    450                 455                 460                 
Thr Leu Pro Asp Ser Cys Glu Ala Ile Gln Val Ala Gly Thr Tyr Thr 
465                 470                 475                 480 
Gln Ala Ser Pro Thr Ala Phe Pro Pro Asn Gln Thr Pro Asn Pro Ser 
                485                 490                 495     
Ala Glu Thr Pro Glu Thr Pro Phe Pro Ser Ser Cys Ala Asp Ala Asn 
            500                 505                 510         
Glu Val Tyr Val Thr Phe Lys Gly Lys Val Thr Thr Gln Trp Gly Glu 
        515                 520                 525             
Ser Val Lys Val Val Gly Ser Thr Pro Glu Leu Gly Ser Trp Asp Val 
    530                 535                 540                 
Lys Lys Ala Val Pro Leu Ser Ala Ser Ala Tyr Thr Glu Ser Asn Pro 
545                 550                 555                 560 
Leu Trp Lys Ile Thr Val Pro Met Lys Ala Gly Gln Ala Val Gln Tyr 
                565                 570                 575     
Lys Phe Ile Arg Val Asn Gly Asp Gly Lys Ala Gln Trp Glu Ser Asp 
            580                 585                 590         
Pro Asn Arg Thr Phe Glu Val Gly Ala Ala Gly Lys Ala Asp Gly Gly 
        595                 600                 605             
Cys Ser Ser Gln Thr Val Glu Gly Thr Trp Arg 
    610                 615                 

<210> 21
<211> 622
<212> PRT
<213> Aspergillus niger


<220> 
<223> Aspergillus niger glucoamylase (mature)

<400> 21
Asn Val Ile Ser Lys Arg Ala Thr Leu Asp Ser Trp Leu Ser Asn Glu 
1               5                   10                  15      
Ala Thr Val Ala Arg Thr Ala Ile Leu Asn Asn Ile Gly Ala Asp Gly 
            20                  25                  30          
Ala Trp Val Ser Gly Ala Asp Ser Gly Ile Val Val Ala Ser Pro Ser 
        35                  40                  45              
Thr Asp Asn Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ser Gly Leu 
    50                  55                  60                  
Val Leu Lys Thr Leu Val Asp Leu Phe Arg Asn Gly Asp Thr Ser Leu 
65                  70                  75                  80  
Leu Ser Thr Ile Glu Asn Tyr Ile Ser Ala Gln Ala Ile Val Gln Gly 
                85                  90                  95      
Ile Ser Asn Pro Ser Gly Asp Leu Ser Ser Gly Ala Gly Leu Gly Glu 
            100                 105                 110         
Pro Lys Phe Asn Val Asp Glu Thr Ala Tyr Thr Gly Ser Trp Gly Arg 
        115                 120                 125             
Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile Gly Phe 
    130                 135                 140                 
Gly Gln Trp Leu Leu Asp Asn Gly Tyr Thr Ser Thr Ala Thr Asp Ile 
145                 150                 155                 160 
Val Trp Pro Leu Val Arg Asn Asp Leu Ser Tyr Val Ala Gln Tyr Trp 
                165                 170                 175     
Asn Gln Thr Gly Tyr Asp Leu Trp Glu Glu Val Asn Gly Ser Ser Phe 
            180                 185                 190         
Phe Thr Ile Ala Val Gln His Arg Ala Leu Val Glu Gly Ser Ala Phe 
        195                 200                 205             
Ala Thr Ala Val Gly Ser Ser Cys Ser Trp Cys Asp Ser Gln Ala Pro 
    210                 215                 220                 
Glu Ile Leu Cys Tyr Leu Gln Ser Phe Trp Thr Gly Ser Phe Ile Leu 
225                 230                 235                 240 
Ala Asn Phe Asp Ser Ser Arg Ser Gly Lys Asp Ala Asn Thr Leu Leu 
                245                 250                 255     
Gly Ser Ile His Thr Phe Asp Pro Glu Ala Ala Cys Asp Asp Ser Thr 
            260                 265                 270         
Phe Gln Pro Cys Ser Pro Arg Ala Leu Ala Asn His Lys Glu Val Val 
        275                 280                 285             
Asp Ser Phe Arg Ser Ile Tyr Thr Leu Asn Asp Gly Leu Ser Asp Ser 
    290                 295                 300                 
Glu Ala Val Ala Val Gly Arg Tyr Pro Glu Asp Thr Tyr Tyr Asn Gly 
305                 310                 315                 320 
Asn Pro Trp Phe Leu Cys Thr Leu Ala Ala Ala Glu Gln Leu Tyr Asp 
                325                 330                 335     
Ala Leu Tyr Gln Trp Asp Lys Gln Gly Ser Leu Glu Val Thr Asp Val 
            340                 345                 350         
Ser Leu Asp Phe Phe Lys Ala Leu Tyr Ser Asp Ala Ala Thr Gly Thr 
        355                 360                 365             
Tyr Ser Ser Ser Ser Ser Thr Tyr Ser Ser Ile Val Asp Ala Val Lys 
    370                 375                 380                 
Thr Phe Ala Asp Gly Phe Val Ser Ile Val Glu Thr His Ala Ala Ser 
385                 390                 395                 400 
Asn Gly Ser Met Ser Glu Gln Tyr Asp Lys Ser Asp Gly Glu Gln Leu 
                405                 410                 415     
Ser Ala Arg Asp Leu Thr Trp Ser Tyr Ala Ala Leu Leu Thr Ala Asn 
            420                 425                 430         
Asn Arg Arg Asn Ser Val Val Pro Ala Ser Trp Gly Glu Thr Ser Ala 
        435                 440                 445             
Ser Ser Val Pro Gly Thr Cys Ala Ala Thr Ser Ala Ile Gly Thr Tyr 
    450                 455                 460                 
Ser Ser Val Thr Val Thr Ser Trp Pro Ser Ile Val Ala Thr Gly Gly 
465                 470                 475                 480 
Thr Thr Thr Thr Ala Thr Pro Thr Gly Ser Gly Ser Val Thr Ser Thr 
                485                 490                 495     
Ser Lys Thr Thr Ala Thr Ala Ser Lys Thr Ser Thr Ser Thr Ser Ser 
            500                 505                 510         
Thr Ser Cys Thr Thr Pro Thr Ala Val Ala Val Thr Phe Asp Leu Thr 
        515                 520                 525             
Ala Thr Thr Thr Tyr Gly Glu Asn Ile Tyr Leu Val Gly Ser Ile Ser 
    530                 535                 540                 
Gln Leu Gly Asp Trp Glu Thr Ser Asp Gly Ile Ala Leu Ser Ala Asp 
545                 550                 555                 560 
Lys Tyr Thr Ser Ser Asp Pro Leu Trp Tyr Val Thr Val Thr Leu Pro 
                565                 570                 575     
Ala Gly Glu Ser Phe Glu Tyr Lys Phe Ile Arg Ile Glu Ser Asp Asp 
            580                 585                 590         
Ser Val Glu Trp Glu Ser Asp Pro Asn Arg Glu Tyr Thr Val Pro Gln 
        595                 600                 605             
Ala Cys Gly Thr Ser Thr Ala Thr Val Thr Asp Thr Trp Arg 
    610                 615                 620         

<210> 22
<211> 612
<212> PRT
<213> Trichoderma reesei


<220> 
<223> T. reesei glucoamylase (mature)

<400> 22
Arg Pro Gly Ser Ser Gly Leu Ser Asp Val Thr Lys Arg Ser Val Asp 
1               5                   10                  15      
Asp Phe Ile Ser Thr Glu Thr Pro Ile Ala Leu Asn Asn Leu Leu Cys 
            20                  25                  30          
Asn Val Gly Pro Asp Gly Cys Arg Ala Phe Gly Thr Ser Ala Gly Ala 
        35                  40                  45              
Val Ile Ala Ser Pro Ser Thr Ile Asp Pro Asp Tyr Tyr Tyr Met Trp 
    50                  55                  60                  
Thr Arg Asp Ser Ala Leu Val Phe Lys Asn Leu Ile Asp Arg Phe Thr 
65                  70                  75                  80  
Glu Thr Tyr Asp Ala Gly Leu Gln Arg Arg Ile Glu Gln Tyr Ile Thr 
                85                  90                  95      
Ala Gln Val Thr Leu Gln Gly Leu Ser Asn Pro Ser Gly Ser Leu Ala 
            100                 105                 110         
Asp Gly Ser Gly Leu Gly Glu Pro Lys Phe Glu Leu Thr Leu Lys Pro 
        115                 120                 125             
Phe Thr Gly Asn Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg 
    130                 135                 140                 
Ala Ile Ala Leu Ile Gly Tyr Ser Lys Trp Leu Ile Asn Asn Asn Tyr 
145                 150                 155                 160 
Gln Ser Thr Val Ser Asn Val Ile Trp Pro Ile Val Arg Asn Asp Leu 
                165                 170                 175     
Asn Tyr Val Ala Gln Tyr Trp Asn Gln Thr Gly Phe Asp Leu Trp Glu 
            180                 185                 190         
Glu Val Asn Gly Ser Ser Phe Phe Thr Val Ala Asn Gln His Arg Ala 
        195                 200                 205             
Leu Val Glu Gly Ala Thr Leu Ala Ala Thr Leu Gly Gln Ser Gly Ser 
    210                 215                 220                 
Ala Tyr Ser Ser Val Ala Pro Gln Val Leu Cys Phe Leu Gln Arg Phe 
225                 230                 235                 240 
Trp Val Ser Ser Gly Gly Tyr Val Asp Ser Asn Ile Asn Thr Asn Glu 
                245                 250                 255     
Gly Arg Thr Gly Lys Asp Val Asn Ser Val Leu Thr Ser Ile His Thr 
            260                 265                 270         
Phe Asp Pro Asn Leu Gly Cys Asp Ala Gly Thr Phe Gln Pro Cys Ser 
        275                 280                 285             
Asp Lys Ala Leu Ser Asn Leu Lys Val Val Val Asp Ser Phe Arg Ser 
    290                 295                 300                 
Ile Tyr Gly Val Asn Lys Gly Ile Pro Ala Gly Ala Ala Val Ala Ile 
305                 310                 315                 320 
Gly Arg Tyr Ala Glu Asp Val Tyr Tyr Asn Gly Asn Pro Trp Tyr Leu 
                325                 330                 335     
Ala Thr Phe Ala Ala Ala Glu Gln Leu Tyr Asp Ala Ile Tyr Val Trp 
            340                 345                 350         
Lys Lys Thr Gly Ser Ile Thr Val Thr Ala Thr Ser Leu Ala Phe Phe 
        355                 360                 365             
Gln Glu Leu Val Pro Gly Val Thr Ala Gly Thr Tyr Ser Ser Ser Ser 
    370                 375                 380                 
Ser Thr Phe Thr Asn Ile Ile Asn Ala Val Ser Thr Tyr Ala Asp Gly 
385                 390                 395                 400 
Phe Leu Ser Glu Ala Ala Lys Tyr Val Pro Ala Asp Gly Ser Leu Ala 
                405                 410                 415     
Glu Gln Phe Asp Arg Asn Ser Gly Thr Pro Leu Ser Ala Leu His Leu 
            420                 425                 430         
Thr Trp Ser Tyr Ala Ser Phe Leu Thr Ala Thr Ala Arg Arg Ala Gly 
        435                 440                 445             
Ile Val Pro Pro Ser Trp Ala Asn Ser Ser Ala Ser Thr Ile Pro Ser 
    450                 455                 460                 
Thr Cys Ser Gly Ala Ser Val Val Gly Ser Tyr Ser Arg Pro Thr Ala 
465                 470                 475                 480 
Thr Ser Phe Pro Pro Ser Gln Thr Pro Lys Pro Gly Val Pro Ser Gly 
                485                 490                 495     
Thr Pro Tyr Thr Pro Leu Pro Cys Ala Thr Pro Thr Ser Val Ala Val 
            500                 505                 510         
Thr Phe His Glu Leu Val Ser Thr Gln Phe Gly Gln Thr Val Lys Val 
        515                 520                 525             
Ala Gly Asn Ala Ala Ala Leu Gly Asn Trp Ser Thr Ser Ala Ala Val 
    530                 535                 540                 
Ala Leu Asp Ala Val Asn Tyr Ala Asp Asn His Pro Leu Trp Ile Gly 
545                 550                 555                 560 
Thr Val Asn Leu Glu Ala Gly Asp Val Val Glu Tyr Lys Tyr Ile Asn 
                565                 570                 575     
Val Gly Gln Asp Gly Ser Val Thr Trp Glu Ser Asp Pro Asn His Thr 
            580                 585                 590         
Tyr Thr Val Pro Ala Val Ala Cys Val Thr Gln Val Val Lys Glu Asp 
        595                 600                 605             
Thr Trp Gln Ser 
    610         

<210> 23
<211> 535
<212> PRT
<213> Botryotinia fuckeliana


<220> 
<223> Botryotinia fuckeliana glucoamylase (mature)

<400> 23
Lys Asn Leu Glu His Pro Lys Leu Ser Thr Arg Thr Thr Gly Thr Ile 
1               5                   10                  15      
Asp Asp Tyr Leu Thr Ala Gln Ser Pro Ile Ser Leu Gln Gly Ile Leu 
            20                  25                  30          
Asn Asn Ile Gly Pro Asp Gly Ser Lys Ala Gln Gly Ala Ser Ala Gly 
        35                  40                  45              
Ile Val Val Ala Ser Pro Ser Thr Val Asp Pro Asn Tyr Phe Tyr Thr 
    50                  55                  60                  
Trp Thr Arg Asp Ser Ala Leu Thr Phe Lys Tyr Leu Ile Asp Thr Gly 
65                  70                  75                  80  
Asn Ser Ser Leu Gln Ser Leu Ile Glu Asp Tyr Val Thr Ala Gln Ala 
                85                  90                  95      
Lys Leu Gln Thr Val Glu Asn Pro Ser Gly Asp Leu Ala Thr Gly Ala 
            100                 105                 110         
Gly Leu Ala Glu Pro Lys Tyr Tyr Thr Asn Glu Thr Ala Phe Leu Gly 
        115                 120                 125             
Glu Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Ile Ala 
    130                 135                 140                 
Leu Ile Ser Phe Gly Asn Asp Arg Leu Thr Leu Gly Gln Asn Asp Thr 
145                 150                 155                 160 
Val Lys Glu Ile Ile Trp Pro Ile Val Arg Asn Asp Leu Ala Tyr Val 
                165                 170                 175     
Ser Gln Tyr Trp Asn Phe Ser Gly Phe Asp Leu Trp Glu Glu Ile Asn 
            180                 185                 190         
Gly Ser Ser Phe Phe Thr Thr Ala Val Gln His Arg Ala Leu Val Glu 
        195                 200                 205             
Gly Ala Lys Phe Ala Thr Ala Leu Gly Glu Ser Cys Asn Asp Cys Glu 
    210                 215                 220                 
Glu Gln Ala Pro Glu Val Leu Cys Met Leu Gln Asp Tyr Trp Asn Gly 
225                 230                 235                 240 
Ala Ser Ile Asn Ser Asn Ile Gln Val Gln Ser Ser Thr Tyr Asp Arg 
                245                 250                 255     
Ser Gly Leu Asp Cys Asn Ser Ile Leu Thr Ser Ile His Thr Phe Asp 
            260                 265                 270         
Pro Asp Gln Ala Val Gly Cys Asp Ser Thr Thr Phe Gln Pro Cys Ser 
        275                 280                 285             
Asp Arg Ala Leu Ala Asn His Lys Val Leu Val Asp Ser Phe Arg Ser 
    290                 295                 300                 
Ile Tyr Thr Ile Asn Ser Asn Ala Ser Thr Gly Gln Ala Ala Ala Ile 
305                 310                 315                 320 
Gly Arg Tyr Ala Glu Asp Val Tyr Gln Gly Gly Asn Pro Trp Tyr Ile 
                325                 330                 335     
Cys Thr Tyr Ala Ala Ala Glu Gln Leu Tyr Asp Ala Leu Trp Thr Tyr 
            340                 345                 350         
Asn Thr Thr Glu Ser Ile Thr Ile Thr Asp Ile Ser Gln Asp Phe Phe 
        355                 360                 365             
Thr Asp Leu Val Pro Gly Ile Glu Thr Gly Thr Phe Ala Ser Asp Ser 
    370                 375                 380                 
Ser Gln Phe Lys Asp Ile Val Lys Ala Met Gln Asp Tyr Ala Asp Gly 
385                 390                 395                 400 
Phe Val Ser Ile Ala Glu Thr Tyr Thr Pro Glu Asp Gly Ala Leu Ala 
                405                 410                 415     
Glu Gln Phe Ser Arg Glu Asn Gly Thr Ala Leu Ser Ala Val Asp Leu 
            420                 425                 430         
Thr Trp Ser Tyr Ala Ser Phe Leu Thr Met Gln Arg Ala Arg Glu Gly 
        435                 440                 445             
Lys Ser Gly Pro Ser Trp Gly Ala Ala Ser Ala Ile Ala Lys Gly Val 
    450                 455                 460                 
Pro Asp Lys Cys Ser Gly Gly Gly Val Lys Gly Ala Tyr Ser Thr Pro 
465                 470                 475                 480 
Ser Ala Thr Ala Phe Pro Gln Ser Glu Glu Gly Thr Phe Gly Thr Lys 
                485                 490                 495     
Thr Ser Ser Thr Ser Asp Thr Gly Thr Gly Thr Gly Thr Val Ser Gly 
            500                 505                 510         
Thr Ala Val Ser Ala Thr Thr Ser Lys Ser Ala Gly Asn Arg Val Met 
        515                 520                 525             
Pro Ile Gly Phe Phe Leu His 
    530                 535 

<210> 24
<211> 575
<212> PRT
<213> Auricularia delicata


<220> 
<223> Auricularia delicata Glucoamylase (mature)

<400> 24
Phe Val Ala Gln Asp Ala Arg Phe Ser Ser Gly Phe Gly Leu Thr Leu 
1               5                   10                  15      
Gly Lys Arg Ala Ala Val Asp Asp Tyr Val Ala Ser Glu Gly Pro Ile 
            20                  25                  30          
Ala Leu Lys Gly Val Leu Asp Asn Ile Gly Pro Asp Gly Pro Lys Ser 
        35                  40                  45              
His Gly Ala Lys Pro Gly Ile Val Val Ala Ser Pro Ser Lys Val Asp 
    50                  55                  60                  
Pro Asp Tyr Val Phe Ala Trp Ile Arg Asp Ser Ala Leu Val Phe Lys 
65                  70                  75                  80  
Ala Leu Ile Asp Arg Phe Val Asp Gly Arg Asp Ala Ser Arg Leu Pro 
                85                  90                  95      
Leu Leu Leu Gln Phe Val Ser Ser Gln Ser Ala Ile Gln Asn Leu Asp 
            100                 105                 110         
Asn Arg Ser Gly Ala Ala Arg Gly Ala Gly Leu Gly Glu Pro Lys Phe 
        115                 120                 125             
Glu Ile Asn Gln Thr Ala Phe Asn Gly Asp Trp Gly Arg Pro Gln Arg 
    130                 135                 140                 
Asp Gly Pro Ala Leu Arg Ala Thr Ala Leu Ile Thr Leu Ser Asn Tyr 
145                 150                 155                 160 
Phe Leu Ser Lys Asn Asn Ala Ser Phe Val Thr Ser Thr Leu Trp Pro 
                165                 170                 175     
Met Ile Gln Leu Asp Leu Asn Tyr Val Ala Thr Tyr Trp Asn Gln Pro 
            180                 185                 190         
Thr Phe Asp Leu Trp Glu Glu Val Asn Gly Gln Ser Phe Phe Thr Thr 
        195                 200                 205             
Ala Val Gln His Arg Ala Leu Arg Glu Gly Ile Ala Leu Ala Gln Thr 
    210                 215                 220                 
Leu Gly Thr Thr Gly Ser Val Ala Thr Trp Ser Thr Gln Ala Ser Asn 
225                 230                 235                 240 
Val Leu Cys Tyr Leu Gln Ser Tyr Trp Thr Pro Ser Gly Ala Phe Ile 
                245                 250                 255     
Asn Ser Asn Thr Asn Ser Gly Arg Ser Gly Ile Asp Val Asn Ser Ile 
            260                 265                 270         
Leu Thr Ser Ile His Thr Phe Ser Pro Asn Thr Gly Cys Asp Ala Val 
        275                 280                 285             
Thr Phe Gln Pro Cys Ser Asp Lys Ala Leu Ala Gly His Leu Ala Val 
    290                 295                 300                 
Val Asn Ser Phe Arg Gly Ser Leu Tyr Pro Ile Asn Ser Gly Ile Pro 
305                 310                 315                 320 
Ala Gly Gln Ala Val Ala Ile Gly Arg Tyr Lys Glu Asp Val Tyr Tyr 
                325                 330                 335     
Asn Gly Asn Pro Trp Tyr Leu Ala Thr Phe Ala Ala Ala Glu Gln Leu 
            340                 345                 350         
Tyr Leu Ala Leu Phe Thr Trp Asp Gln Gln Lys Ser Ile Ala Val Thr 
        355                 360                 365             
Ala Ile Ser Gln Pro Phe Phe Ala Gln Phe Ile Pro Asn Ile Ala Thr 
    370                 375                 380                 
Gly Thr Tyr Ala Ser Ser Ser Ser Gln Tyr Ala Thr Leu Thr Ser Lys 
385                 390                 395                 400 
Ile Arg Glu Phe Ala Asp Gly Phe Ile Ala Val Asn Gln Lys Tyr Thr 
                405                 410                 415     
Pro Ala Asn Gly Ala Leu Ala Glu Gln Phe Thr Arg Ala Asn Gly Thr 
            420                 425                 430         
Pro Ile Ser Ala Ser Asp Leu Thr Trp Ser Tyr Ala Ser Ala Leu Thr 
        435                 440                 445             
Ala Phe Asp Arg Arg Ala Gly Ile Val Pro Gly Ala Trp Pro Ala Thr 
    450                 455                 460                 
Gly Leu Thr Val Ala Pro Ser Cys Ser Thr Ala Gly Gly Gly Asn Ser 
465                 470                 475                 480 
Thr Ile Thr Phe Lys Val Thr Ala Gln Thr Val Phe Gly Glu Asn Ile 
                485                 490                 495     
Tyr Leu Thr Gly Ser Val Ala Ala Leu Lys Asn Trp Ser Pro Glu Asn 
            500                 505                 510         
Ala Leu Gly Pro Leu Ala Asn Pro Asn Tyr Pro Gln Trp Gln Ile Thr 
        515                 520                 525             
Val Thr Val Pro Ala Ser Thr Ala Ile Glu Tyr Lys Tyr Ile Arg Lys 
    530                 535                 540                 
Asn Gly Gly Ser Val Val Trp Glu Ser Asp Pro Asn Arg Ser Ile Val 
545                 550                 555                 560 
Ser Pro Ala Ala Gly Glu Ser Ala Thr Val Thr Asp Thr Trp Arg 
                565                 570                 575 

<210> 25
<211> 617
<212> PRT
<213> Talaromyces stipitatus


<220> 
<223> Taloromyces stipitatus glucoamylase (mature)

<400> 25
Ala Pro Gly Leu Ser Pro Arg Ala Ser Thr Ser Leu Asp Ala Trp Leu 
1               5                   10                  15      
Ala Thr Glu Thr Thr Val Ser Leu Ser Gly Ile Leu Ala Asn Ile Gly 
            20                  25                  30          
Ala Asp Gly Ala Tyr Ser Lys Ser Ala Lys Pro Gly Val Val Ile Ala 
        35                  40                  45              
Ser Pro Ser Thr Asp Asn Pro Asn Tyr Tyr Tyr Thr Trp Thr Arg Asp 
    50                  55                  60                  
Ser Ala Leu Thr Leu Lys Val Leu Ile Asp Leu Phe Arg Asn Gly Asn 
65                  70                  75                  80  
Leu Gly Leu Gln Thr Val Ile Glu Glu Tyr Val Asn Ala Gln Ala Tyr 
                85                  90                  95      
Leu Gln Thr Val Ser Asn Pro Ser Gly Asp Leu Ser Ser Gly Ala Gly 
            100                 105                 110         
Leu Ala Glu Pro Lys Phe Asn Val Asp Met Ser Ala Phe Thr Gly Ser 
        115                 120                 125             
Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Ile Ala Leu 
    130                 135                 140                 
Ile Asp Phe Gly Asn Trp Leu Ile Glu Asn Gly Tyr Thr Ser Leu Ala 
145                 150                 155                 160 
Ala Asn Asn Ile Trp Pro Ile Val Arg Asn Asp Leu Ser Tyr Val Ala 
                165                 170                 175     
Gln Tyr Trp Ser Gln Ser Gly Phe Asp Leu Trp Glu Glu Val Asn Ser 
            180                 185                 190         
Met Ser Phe Phe Thr Val Ala Asn Gln His Arg Ser Leu Val Glu Gly 
        195                 200                 205             
Ser Thr Phe Ala Ala Lys Val Gly Ala Ser Cys Ser Trp Cys Asp Ser 
    210                 215                 220                 
Gln Ala Pro Gln Ile Leu Cys Tyr Met Gln Thr Phe Trp Thr Gly Ser 
225                 230                 235                 240 
Tyr Met Asn Ala Asn Thr Gly Gly Gly Arg Ser Gly Lys Asp Ala Asn 
                245                 250                 255     
Thr Val Leu Thr Ser Ile Ala Thr Phe Asp Pro Glu Ala Thr Cys Asp 
            260                 265                 270         
Asp Val Thr Phe Gln Pro Cys Ser Pro Arg Ala Leu Ala Asn His Lys 
        275                 280                 285             
Val Tyr Thr Asp Ser Phe Arg Ser Val Tyr Gly Leu Asn Ser Gly Ile 
    290                 295                 300                 
Ala Glu Gly Val Ala Val Ala Val Gly Arg Tyr Pro Glu Asp Ser Tyr 
305                 310                 315                 320 
Tyr Asn Gly Asn Pro Trp Phe Leu Ser Asn Leu Ala Ala Ala Glu Gln 
                325                 330                 335     
Leu Tyr Asp Ala Ile Tyr Gln Trp Asn Lys Ile Gly Ser Ile Thr Ile 
            340                 345                 350         
Thr Ser Thr Ser Leu Ala Phe Phe Lys Asp Val Tyr Ser Ser Ala Ala 
        355                 360                 365             
Val Gly Thr Tyr Ala Ser Gly Ser Ser Ala Phe Thr Ser Ile Ile Asn 
    370                 375                 380                 
Ala Val Lys Thr Tyr Ala Asp Gly Tyr Ile Ser Val Val Gln Ser His 
385                 390                 395                 400 
Ala Met Asn Asn Gly Ser Leu Ser Glu Gln Phe Asp Lys Asn Thr Gly 
                405                 410                 415     
Ala Glu Leu Ser Ala Arg Asp Leu Thr Trp Ser Tyr Ala Ala Leu Leu 
            420                 425                 430         
Thr Ala Asn Met Arg Arg Asn Gly Val Val Pro Pro Ser Trp Gly Ala 
        435                 440                 445             
Ala Ser Ala Thr Ser Ile Pro Ser Ser Cys Thr Thr Gly Ser Ala Ile 
    450                 455                 460                 
Gly Thr Tyr Ser Thr Pro Thr Ala Thr Ser Trp Pro Ser Thr Leu Thr 
465                 470                 475                 480 
Ser Gly Thr Gly Ser Pro Gly Ser Thr Thr Ser Ala Thr Gly Ser Val 
                485                 490                 495     
Ser Thr Ser Val Ser Ala Thr Thr Thr Ser Ala Gly Ser Cys Thr Thr 
            500                 505                 510         
Pro Thr Ser Val Ala Val Thr Phe Asp Glu Ile Ala Thr Thr Ser Tyr 
        515                 520                 525             
Gly Glu Asn Val Tyr Ile Val Gly Ser Ile Ser Gln Leu Gly Ser Trp 
    530                 535                 540                 
Asn Thr Ala Asn Ala Ile Ala Leu Ser Ala Ser Lys Tyr Thr Thr Ser 
545                 550                 555                 560 
Asn Asn Leu Trp Tyr Val Thr Ile Asn Leu Pro Ala Gly Thr Thr Phe 
                565                 570                 575     
Gln Tyr Lys Tyr Ile Arg Lys Glu Ser Asp Gly Thr Val Lys Trp Glu 
            580                 585                 590         
Ser Asp Pro Asn Arg Ser Tyr Thr Val Pro Ser Ala Cys Gly Val Ser 
        595                 600                 605             
Thr Ala Thr Glu Asn Asp Thr Trp Arg 
    610                 615         

<210> 26
<211> 576
<212> PRT
<213> Piriformospora indica


<220> 
<223> Piriformospora indica glucoamylase (mature)

<400> 26
Arg Ala Ser Pro Arg Glu Asn Pro Phe Asn Lys Arg Val Ser Tyr Thr 
1               5                   10                  15      
Asn Val Ala Asp Phe Asp Thr Phe Glu Thr Pro Ile Ala Leu Ala Gly 
            20                  25                  30          
Leu Tyr Ala Asn Ile Gly Pro Asp Gly Ala Lys Ser Gln Gly Ala Lys 
        35                  40                  45              
Ala Gly Val Val Ile Ala Ser Pro Ser Thr Ser Asp Pro Asp Tyr Leu 
    50                  55                  60                  
Tyr Thr Trp Thr Arg Asp Ala Ser Leu Val Phe Lys Tyr Ile Val Asp 
65                  70                  75                  80  
Arg Phe Thr Ser Gly Arg Asp Ser Ser Leu Arg Thr Lys Ile Asp Asn 
                85                  90                  95      
Phe Val Gly His Val Gly Arg Ile Gln Gln Val Thr Asn Pro Ser Gly 
            100                 105                 110         
Thr Val Ser Thr Gly Gly Leu Gly Glu Pro Lys Phe Met Ile Ser Glu 
        115                 120                 125             
Ala Ala Phe Thr Gly Glu Trp Gly Arg Pro Gln Arg Asp Gly Pro Ala 
    130                 135                 140                 
Leu Arg Ala Thr Thr Leu Ile Ala Tyr Ala Asn Trp Leu Arg Ala Asn 
145                 150                 155                 160 
Ser Asn Thr Thr His Val Gln Asn Val Leu Trp Pro Ile Ile Ser Leu 
                165                 170                 175     
Asp Leu Asn Tyr Val Ser Asn Asn Trp Asn Ser Thr Thr Phe Asp Leu 
            180                 185                 190         
Trp Glu Glu Val Ser Gly Ala Ser Phe Phe Thr Thr Ala Ala Gln His 
        195                 200                 205             
Arg Ala Leu Arg Glu Gly Ile Ala Leu Ala Thr Ala Leu Gly Ala Pro 
    210                 215                 220                 
Ser Ser Thr Ile Thr Ala Trp Thr Thr Gln Ala Gln Asn Leu Leu Cys 
225                 230                 235                 240 
Phe Leu Gln Thr Tyr Trp Ser Pro Glu Ser Gly Phe Ile Val Ala Asn 
                245                 250                 255     
Val Asp Pro Ile Asn Gly Ala Ile Arg Ser Gly Lys Asp Ala Asn Thr 
            260                 265                 270         
Val Leu Thr Thr Ile His Thr Phe Asp Pro Ala Ala Gly Cys Asp Ser 
        275                 280                 285             
Ser Thr Phe Gln Pro Cys Ser Asp Lys Ala Leu Ala Asn Leu Lys Val 
    290                 295                 300                 
Tyr Val Asp Ser Phe Arg Ser Ile Tyr Pro Ile Asn Tyr Gly Ile Ala 
305                 310                 315                 320 
Ser Asn Ala Ala Val Ala Thr Gly Arg Tyr Pro Glu Asp Val Tyr Tyr 
                325                 330                 335     
Gly Gly Asn Pro Trp Tyr Leu Thr Thr Phe Ala Val Ala Glu Gln Leu 
            340                 345                 350         
Tyr Arg Ala Leu Gln Val Trp Asp Ala Glu Gly Lys Gly Ile Glu Val 
        355                 360                 365             
Thr Ser Ile Ser Leu Pro Phe Phe Gln Gln Phe Asn Ser Ser Ala Thr 
    370                 375                 380                 
Val Thr Thr Ile Pro Ala Gly Ser Ala Ser Tyr Thr Thr Leu Thr Asn 
385                 390                 395                 400 
Ala Ile Lys Thr Phe Ala Asp Gly Phe Ala Leu Lys Gly Ala Gln Phe 
                405                 410                 415     
Val Pro Ser Ser Gly Ala Leu Ala Glu Gln Tyr Ala Arg Asn Asp Gly 
            420                 425                 430         
Thr Pro Val Ser Ala Val Asp Leu Thr Trp Ser Tyr Ala Ser Val Leu 
        435                 440                 445             
Thr Met Phe Asp Ala His Asp Arg Leu Ala Val Asp Ser Trp Gly Ala 
    450                 455                 460                 
Ala Gly Leu Thr Val Pro Thr Thr Cys Ser Ser Gly Asp Gly Lys Ser 
465                 470                 475                 480 
Ala Thr Val Ile Phe Lys Glu Thr Ala Thr Thr Ile Trp Gly Glu Asn 
                485                 490                 495     
Ile Tyr Leu Val Gly Asn Ile Glu Ala Leu Lys Asn Trp Asn Thr Asn 
            500                 505                 510         
Asn Pro Ile Gly Pro Leu Ser Thr Thr Thr Tyr Pro Val Trp Thr Thr 
        515                 520                 525             
Leu Val Ser Ile Pro Ala Asn Thr His Phe Glu Tyr Lys Phe Ile Arg 
    530                 535                 540                 
Lys Phe Asn Gly Thr Val Thr Trp Glu Ser Gly Ala Asn Arg Trp Asn 
545                 550                 555                 560 
Ala Thr Gly Ala Ala Gly Thr Arg Leu Thr Leu Asn Asn Val Trp Lys 
                565                 570                 575     



<210> 27
<211> 497
<212> PRT
<213> Saccharomycopsis fibuligera


<220> 
<223> Saccharomycopsis fibuligera glucoamylase (mature)

<400> 27
Val Pro Val Glu Leu Asp Lys Arg Asn Thr Gly His Phe Gln Ala Tyr 
1               5                   10                  15      
Ser Gly Tyr Thr Val Ala Arg Ser Asn Phe Thr Gln Trp Ile His Glu 
            20                  25                  30          
Gln Pro Ala Val Ser Trp Tyr Tyr Leu Leu Gln Asn Ile Asp Tyr Pro 
        35                  40                  45              
Glu Gly Gln Phe Lys Ser Ala Lys Pro Gly Val Val Val Ala Ser Pro 
    50                  55                  60                  
Ser Thr Ser Glu Pro Asp Tyr Phe Tyr Gln Trp Thr Arg Asp Thr Ala 
65                  70                  75                  80  
Ile Thr Phe Leu Ser Leu Ile Ala Glu Val Glu Asp His Ser Phe Ser 
                85                  90                  95      
Asn Thr Thr Leu Ala Lys Val Val Glu Tyr Tyr Ile Ser Asn Thr Tyr 
            100                 105                 110         
Thr Leu Gln Arg Val Ser Asn Pro Ser Gly Asn Phe Asp Ser Pro Asn 
        115                 120                 125             
His Asp Gly Leu Gly Glu Pro Lys Phe Asn Val Asp Asp Thr Ala Tyr 
    130                 135                 140                 
Thr Ala Ser Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Leu Arg Ala 
145                 150                 155                 160 
Tyr Ala Ile Ser Arg Tyr Leu Asn Ala Val Ala Lys His Asn Asn Gly 
                165                 170                 175     
Lys Leu Leu Leu Ala Gly Gln Asn Gly Ile Pro Tyr Ser Ser Ala Ser 
            180                 185                 190         
Asp Ile Tyr Trp Lys Ile Ile Lys Pro Asp Leu Gln His Val Ser Thr 
        195                 200                 205             
His Trp Ser Thr Ser Gly Phe Asp Leu Trp Glu Glu Asn Gln Gly Thr 
    210                 215                 220                 
His Phe Phe Thr Ala Leu Val Gln Leu Lys Ala Leu Ser Tyr Gly Ile 
225                 230                 235                 240 
Pro Leu Ser Lys Thr Tyr Asn Asp Pro Gly Phe Thr Ser Trp Leu Glu 
                245                 250                 255     
Lys Gln Lys Asp Ala Leu Asn Ser Tyr Ile Asn Ser Ser Gly Phe Val 
            260                 265                 270         
Asn Ser Gly Lys Lys His Ile Val Glu Ser Pro Gln Leu Ser Ser Arg 
        275                 280                 285             
Gly Gly Leu Asp Ser Ala Thr Tyr Ile Ala Ala Leu Ile Thr His Asp 
    290                 295                 300                 
Ile Gly Asp Asp Asp Thr Tyr Thr Pro Phe Asn Val Asp Asn Ser Tyr 
305                 310                 315                 320 
Val Leu Asn Ser Leu Tyr Tyr Leu Leu Val Asp Asn Lys Asn Arg Tyr 
                325                 330                 335     
Lys Ile Asn Gly Asn Tyr Lys Ala Gly Ala Ala Val Gly Arg Tyr Pro 
            340                 345                 350         
Glu Asp Val Tyr Asn Gly Val Gly Thr Ser Glu Gly Asn Pro Trp Gln 
        355                 360                 365             
Leu Ala Thr Ala Tyr Ala Gly Gln Thr Phe Tyr Thr Leu Ala Tyr Asn 
    370                 375                 380                 
Ser Leu Lys Asn Lys Lys Asn Leu Val Ile Glu Lys Leu Asn Tyr Asp 
385                 390                 395                 400 
Leu Tyr Asn Ser Phe Ile Ala Asp Leu Ser Lys Ile Asp Ser Ser Tyr 
                405                 410                 415     
Ala Ser Lys Asp Ser Leu Thr Leu Thr Tyr Gly Ser Asp Asn Tyr Lys 
            420                 425                 430         
Asn Val Ile Lys Ser Leu Leu Gln Phe Gly Asp Ser Phe Leu Lys Val 
        435                 440                 445             
Leu Leu Asp His Ile Asp Asp Asn Gly Gln Leu Thr Glu Glu Ile Asn 
    450                 455                 460                 
Arg Tyr Thr Gly Phe Gln Ala Gly Ala Val Ser Leu Thr Trp Ser Ser 
465                 470                 475                 480 
Gly Ser Leu Leu Ser Ala Asn Arg Ala Arg Asn Lys Leu Ile Glu Leu 
                485                 490                 495     
Leu 
    

<210> 28
<211> 785
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> S. cerevisiae diastaticus glucoamylase (mature)

<400> 28
Phe Pro Thr Ala Leu Val Pro Arg Gly Ser Ser Ser Ser Asn Ile Thr 
1               5                   10                  15      
Ser Ser Gly Pro Ser Ser Thr Pro Phe Ser Ser Ala Thr Glu Ser Phe 
            20                  25                  30          
Ser Thr Gly Thr Thr Val Thr Pro Ser Ser Ser Lys Tyr Pro Gly Ser 
        35                  40                  45              
Lys Thr Glu Thr Ser Val Ser Ser Thr Thr Glu Thr Thr Ile Val Pro 
    50                  55                  60                  
Thr Thr Thr Thr Thr Ser Val Ile Thr Pro Ser Thr Thr Thr Ile Thr 
65                  70                  75                  80  
Thr Thr Val Cys Ser Thr Gly Thr Asn Ser Ala Gly Glu Thr Thr Ser 
                85                  90                  95      
Gly Cys Ser Pro Lys Thr Ile Thr Thr Thr Val Pro Cys Ser Thr Ser 
            100                 105                 110         
Pro Ser Glu Thr Ala Ser Glu Ser Thr Thr Thr Ser Pro Thr Thr Pro 
        115                 120                 125             
Val Thr Thr Val Val Ser Thr Thr Val Val Thr Thr Glu Tyr Ser Thr 
    130                 135                 140                 
Ser Thr Lys Gln Gly Gly Glu Ile Thr Thr Thr Phe Val Thr Lys Asn 
145                 150                 155                 160 
Ile Pro Thr Thr Tyr Leu Thr Thr Ile Ala Pro Thr Ser Ser Val Thr 
                165                 170                 175     
Thr Val Thr Asn Phe Thr Pro Thr Thr Ile Thr Thr Thr Val Cys Ser 
            180                 185                 190         
Thr Gly Thr Asn Ser Ala Gly Glu Thr Thr Ser Gly Cys Ser Pro Lys 
        195                 200                 205             
Thr Val Thr Thr Thr Val Pro Cys Ser Thr Gly Thr Gly Glu Tyr Thr 
    210                 215                 220                 
Thr Glu Ala Thr Ala Pro Val Thr Thr Ala Val Thr Thr Thr Val Val 
225                 230                 235                 240 
Thr Thr Glu Ser Ser Thr Gly Thr Asn Ser Ala Gly Lys Thr Thr Thr 
                245                 250                 255     
Ser Tyr Thr Thr Lys Ser Val Pro Thr Thr Tyr Val Phe Asp Phe Gly 
            260                 265                 270         
Lys Gly Ile Leu Asp Gln Ser Cys Gly Gly Val Phe Ser Asn Asn Gly 
        275                 280                 285             
Ser Ser Gln Val Gln Leu Arg Asp Val Val Leu Met Asn Gly Thr Val 
    290                 295                 300                 
Val Tyr Asp Ser Asn Gly Ala Trp Asp Ser Ser Ala Leu Glu Glu Trp 
305                 310                 315                 320 
Leu Gln Arg Gln Lys Lys Val Ser Ile Glu Arg Ile Phe Glu Asn Ile 
                325                 330                 335     
Gly Pro Ser Ala Val Tyr Pro Ser Ile Leu Pro Gly Val Val Ile Ala 
            340                 345                 350         
Ser Pro Ser Gln Thr His Pro Asp Tyr Phe Tyr Gln Trp Ile Arg Asp 
        355                 360                 365             
Ser Ala Leu Thr Ile Asn Ser Ile Val Ser His Ser Ala Asp Pro Ala 
    370                 375                 380                 
Ile Glu Thr Leu Leu Gln Tyr Leu Asn Val Ser Phe His Leu Gln Arg 
385                 390                 395                 400 
Thr Asn Asn Thr Leu Gly Ala Gly Ile Gly Tyr Thr Asn Asp Thr Val 
                405                 410                 415     
Ala Leu Gly Asp Pro Lys Trp Asn Val Asp Asn Thr Ala Phe Thr Glu 
            420                 425                 430         
Pro Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Leu Arg Ser Ile Ala 
        435                 440                 445             
Ile Leu Lys Ile Ile Asp Tyr Ile Lys Gln Ser Gly Thr Asp Leu Gly 
    450                 455                 460                 
Ala Lys Tyr Pro Phe Gln Ser Thr Ala Asp Ile Phe Asp Asp Ile Val 
465                 470                 475                 480 
Arg Trp Asp Leu Arg Phe Ile Ile Asp His Trp Asn Ser Ser Gly Phe 
                485                 490                 495     
Asp Leu Trp Glu Glu Val Asn Gly Met His Phe Phe Thr Leu Leu Val 
            500                 505                 510         
Gln Leu Ser Ala Val Asp Arg Ser Leu Ser Tyr Phe Asn Ala Ser Glu 
        515                 520                 525             
Arg Ser Ser Pro Phe Val Glu Glu Leu Arg Gln Thr Arg Arg Asp Ile 
    530                 535                 540                 
Ser Lys Phe Leu Val Asp Pro Ala Asn Gly Phe Ile Asn Gly Lys Tyr 
545                 550                 555                 560 
Asn Tyr Ile Val Glu Thr Pro Met Ile Ala Asp Thr Leu Arg Ser Gly 
                565                 570                 575     
Leu Asp Ile Ser Thr Leu Leu Ala Ala Asn Thr Val His Asp Ala Pro 
            580                 585                 590         
Ser Ala Ser His Leu Pro Phe Asp Ile Asn Asp Pro Ala Val Leu Asn 
        595                 600                 605             
Thr Leu His His Leu Met Leu His Met Arg Ser Ile Tyr Pro Ile Asn 
    610                 615                 620                 
Asp Ser Ser Lys Asn Ala Thr Gly Ile Ala Leu Gly Arg Tyr Pro Glu 
625                 630                 635                 640 
Asp Val Tyr Asp Gly Tyr Gly Val Gly Glu Gly Asn Pro Trp Val Leu 
                645                 650                 655     
Ala Thr Cys Ala Ala Ser Thr Thr Leu Tyr Gln Leu Ile Tyr Arg His 
            660                 665                 670         
Ile Ser Glu Gln His Asp Leu Val Val Pro Met Asn Asn Asp Cys Ser 
        675                 680                 685             
Asn Ala Phe Trp Ser Glu Leu Val Phe Ser Asn Leu Thr Thr Leu Gly 
    690                 695                 700                 
Asn Asp Glu Gly Tyr Leu Ile Leu Glu Phe Asn Thr Pro Ala Phe Asn 
705                 710                 715                 720 
Gln Thr Ile Gln Lys Ile Phe Gln Leu Ala Asp Ser Phe Leu Val Lys 
                725                 730                 735     
Leu Lys Ala His Val Gly Thr Asp Gly Glu Leu Ser Glu Gln Phe Asn 
            740                 745                 750         
Lys Tyr Thr Gly Phe Met Gln Gly Ala Gln His Leu Thr Trp Ser Tyr 
        755                 760                 765             
Thr Ser Phe Trp Asp Ala Tyr Gln Ile Arg Gln Glu Val Leu Gln Ser 
    770                 775                 780                 
Leu 
785 

<210> 29
<211> 20
<212> PRT
<213> Amorphotheca resinae 


<220> 
<223> Amorphotheca resinae glucoamylase signal sequence

<400> 29
Met His Ser Phe Thr Ser Leu Leu Leu Val Ser Gly Leu Ala Leu Gln 
1               5                   10                  15      
Thr Ala Val Gly 
            20  

<210> 30
<211> 20
<212> PRT
<213> Corynascus sepedonium


<220> 
<223> Corynascus sepedonium glucoamylase signal sequence

<400> 30
Met His Ala Leu Ser Ser Leu Val Val Leu Gly Thr Cys Ala Val Gln 
1               5                   10                  15      
Thr Ala Leu Gly 
            20  

<210> 31
<211> 18
<212> PRT
<213> Aspergillus niger


<220> 
<223> Aspergillus niger glucoamylase signal sequence

<400> 31
Met Ser Phe Arg Ser Leu Leu Ala Leu Ser Gly Leu Val Cys Thr Gly 
1               5                   10                  15      
Leu Ala 
        

<210> 32
<211> 20
<212> PRT
<213> Aspergillus niger


<220> 
<223> T. reesei glucoamylase signal sequence

<400> 32
Met His Val Leu Ser Thr Ala Val Leu Leu Gly Ser Val Ala Val Gln 
1               5                   10                  15      
Lys Val Leu Gly 
            20  

<210> 33
<211> 17
<212> PRT
<213> Botryotinia fuckeliana


<220> 
<223> Botryotinia fuckeliana glucoamylase signal sequence

<400> 33
Met Leu Trp Glu Arg Ala Thr Ala Phe Val Ala Gly Ala Leu Ser Ser 
1               5                   10                  15      
Ala 
    

<210> 34
<211> 15
<212> PRT
<213> Auricularia delicata


<220> 
<223> Auricularia delicata glucoamylase signal sequence

<400> 34
Met Arg Leu Pro Ser Val Ala Phe Phe Ala Ala Ala Ala Ser Ala 
1               5                   10                  15  

<210> 35
<211> 20
<212> PRT
<213> Talaromyces stipitatus


<220> 
<223> Taloromyces stipitatus glucoamylase signal sequence

<400> 35
Met Thr Arg Leu Ser Ser Val Leu Cys Ala Leu Ala Ala Leu Gly Gln 
1               5                   10                  15      
Thr Ala Leu Ala 
            20  

<210> 36
<211> 20
<212> PRT
<213> Piriformospora indica


<220> 
<223> Piriformospora indica glucoamylase signal sequence

<400> 36
Met Leu Phe Thr Ser Val Phe Ser Cys Leu Leu Leu Leu Ser Pro Gly 
1               5                   10                  15      
Gly Ala Leu Ala 
            20  

<210> 37
<211> 17
<212> PRT
<213> Punctularia strigosozonata


<220> 
<223> Punctularia strigosozonata glucoamylase signal sequence

<400> 37
Met Leu Ser Ser Leu Ile Val Ser Gly Leu Leu Ala Ser Gly Val Cys 
1               5                   10                  15      
Ala 
    

<210> 38
<211> 18
<212> PRT
<213> Saccharomycopsis fibuligera - Sfib -  1.381


<220> 
<223> Saccharomycopsis fibuligera glucoamylase signal sequence

<400> 38
Met Ile Arg Leu Thr Val Phe Leu Thr Ala Val Phe Ala Ala Val Ala 
1               5                   10                  15      
Ser Cys 
        

<210> 39
<211> 21
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> S. cerevisiae/diastaticus glucoamylase signal sequence

<400> 39
Met Gln Arg Pro Phe Leu Leu Ala Tyr Leu Val Leu Ser Leu Leu Phe 
1               5                   10                  15      
Asn Ser Ala Leu Gly 
            20      

<210> 40
<211> 18
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> S. cerevisiae AGA2 glucoamylase signal sequence

<400> 40
Met Gln Leu Leu Arg Cys Phe Ser Ile Phe Ser Val Ile Ala Ser Val 
1               5                   10                  15      
Leu Ala 
        

<210> 41
<211> 19
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> S. cerevisiae EXG1 glucoamylase signal sequence

<400> 41
Met Leu Ser Leu Lys Thr Leu Leu Cys Thr Leu Leu Thr Val Ser Ser 
1               5                   10                  15      
Val Leu Ala 
            

<210> 42
<211> 19
<212> PRT
<213> Saccharomyces cerevisiae


<220> 
<223> S. cerevisiae Mfalfa signal sequence

<400> 42
Met Arg Phe Pro Ser Ile Phe Thr Ala Val Leu Phe Ala Ala Ser Ser 
1               5                   10                  15      
Ala Leu Ala 
            

<210> 43
<211> 44
<212> PRT
<213> Trichoderma reesei


<220> 
<223> T. reesei Xyn2 signal sequence

<400> 43
Met Val Ser Phe Thr Ser Leu Leu Ala Ala Ser Pro Pro Ser Arg Ala 
1               5                   10                  15      
Ser Cys Arg Pro Ala Ala Glu Val Glu Ser Val Ala Val Glu Lys Arg 
            20                  25                  30          
Gln Thr Ile Gln Pro Gly Thr Gly Tyr Asn Asn Gly 
        35                  40                  

<210> 44
<211> 17
<212> PRT
<213> Aspergillus niger


<220> 
<223> S. cerevisiae/diastaticus glucoamylase signal sequence

<400> 44
Met Val Lys Ser Ile Leu Ala Ser Val Phe Phe Ala Ala Thr Ala Leu 
1               5                   10                  15      
Ala 
    

<210> 45
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer DBC-16841

<400> 45
cttatcgata ccgtcgacct cgaggggggg cccggtaccc agcttttgtt gggccagaaa     60

aaggaagtgt                                                            70


<210> 46
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer DBC-16903

<400> 46
taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct ggaggaaatg     60

agaaatgaga                                                            70


<210> 47
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer DBC-16904

<400> 47
taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct ggaggaaatg     60

agaaatgaga                                                            70


<210> 48
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer DBC-16844

<400> 48
gaacaaaagc tgggtaccgg                                                20


<210> 49
<211> 4967
<212> DNA
<213> Artificial Sequence


<220> 
<223> plasmid pRS313

<400> 49
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca       60

cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg      120

ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc      180

accataattc cgttttaaga gcttggtgag cgctaggagt cactgccagg tatcgtttga      240

acacggcatt agtcagggaa gtcataacac agtcctttcc cgcaattttc tttttctatt      300

actcttggcc tcctctagta cactctatat ttttttatgc ctcggtaatg attttcattt      360

ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat tatcacataa      420

tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa aaaatgagca      480

ggcaagataa acgaaggcaa agatgacaga gcagaaagcc ctagtaaagc gtattacaaa      540

tgaaaccaag attcagattg cgatctcttt aaagggtggt cccctagcga tagagcactc      600

gatcttccca gaaaaagagg cagaagcagt agcagaacag gccacacaat cgcaagtgat      660

taacgtccac acaggtatag ggtttctgga ccatatgata catgctctgg ccaagcattc      720

cggctggtcg ctaatcgttg agtgcattgg tgacttacac atagacgacc atcacaccac      780

tgaagactgc gggattgctc tcggtcaagc ttttaaagag gccctactgg cgcgtggagt      840

aaaaaggttt ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga      900

tctttcgaac aggccgtacg cagttgtcga acttggtttg caaagggaga aagtaggaga      960

tctctcttgc gagatgatcc cgcattttct tgaaagcttt gcagaggcta gcagaattac     1020

cctccacgtt gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa     1080

ggctcttgcg gttgccataa gagaagccac ctcgcccaat ggtaccaacg atgttccctc     1140

caccaaaggt gttcttatgt agtgacaccg attatttaaa gctgcagcat acgatatata     1200

tacatgtgta tatatgtata cctatgaatg tcagtaagta tgtatacgaa cagtatgata     1260

ctgaagatga caaggtaatg catcattcta tacgtgtcat tctgaacgag gcgcgctttc     1320

cttttttctt tttgcttttt cttttttttt ctcttgaact cgacggatca tatgcggtgt     1380

gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata     1440

ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg     1500

aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc     1560

cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa     1620

ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt     1680

cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac     1740

ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta     1800

gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg     1860

cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc     1920

gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa ggggggatgt gctgcaaggc     1980

gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg     2040

aattgtaata cgactcacta tagggcgaat tggagctcca ccgcggtggc ggccgctcta     2100

gaactagtgg atcccccggg ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc     2160

tcgagggggg gcccggtacc cagcttttgt tccctttagt gagggttaat tccgagcttg     2220

gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac     2280

aacataggag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc     2340

acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg     2400

cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct     2460

tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac     2520

tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga     2580

gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat     2640

aggctcggcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac     2700

ccgacaggac tataaagata ccaggcgttc ccccctggaa gctccctcgt gcgctctcct     2760

gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg     2820

ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg     2880

ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt     2940

cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg     3000

attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac     3060

ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga     3120

aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt     3180

gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt     3240

tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga     3300

ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc     3360

taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct     3420

atctcagcga tctgtctatt tcgttcatcc atagttgcct gactgcccgt cgtgtagata     3480

actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca     3540

cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga     3600

agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga     3660

gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg     3720

gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga     3780

gttacatgat cccccatgtt gtgaaaaaaa gcggttagct ccttcggtcc tccgatcgtt     3840

gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct     3900

cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca     3960

ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat     4020

accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga     4080

aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc     4140

aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg     4200

caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc     4260

ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt     4320

gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca     4380

cctgggtcct tttcatcacg tgctataaaa ataattataa tttaaatttt ttaatataaa     4440

tatataaatt aaaaatagaa agtaaaaaaa gaaattaaag aaaaaatagt ttttgttttc     4500

cgaagatgta aaagactcta gggggatcgc caacaaatac taccttttat cttgctcttc     4560

ctgctctcag gtattaatgc cgaattgttt catcttgtct gtgtagaaga ccacacacga     4620

aaatcctgtg attttacatt ttacttatcg ttaatcgaat gtatatctat ttaatctgct     4680

tttcttgtct aataaatata tatgtaaagt acgctttttg ttgaaatttt ttaaaccttt     4740

gtttattttt ttttcttcat tccgtaactc ttctaccttc tttatttact ttctaaaatc     4800

caaatacaaa acataaaaat aaataaacac agagtaaatt cccaaattat tccatcatta     4860

aaagatacga ggcgcgtgta agttacaggc aagcgatccg tcctaagaaa ccattattat     4920

catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc                   4967


<210> 50
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> connector sequence

<400> 50
tcctgtacgc actttgtccc acaaattccc gattccgcaa tttgttcgcc                50


<210> 51
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> connector sequence

<400> 51
gaacaggata tttcgatctt ggatacgtac tcgcttgtgt ctggtttcgt                50


<210> 52
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> integration target sequence

<400> 52
agaaaactct tagcttttcc                                                20


<210> 53
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> integration target sequence

<400> 53
caatatggta tgccgagtct                                                20


