                         SEQUENCE LISTING

<110>  DSM IP Assets B.V.
 
<120>  Production of trans-retinal

<130>  32582-WO-PCT

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  787
<212>  PRT
<213>  Ustilago maydis

<400>  1

Met Val Lys Gly Ser Ser Asn Arg Arg Gln His Ser Ala Ser Leu Gln 
1               5                   10                  15      


Gly Leu Pro Ser Ser Gln His Cys Ala Pro Val Ile Ser Ile Pro Ser 
            20                  25                  30          


Pro Pro Pro Pro Ala Glu Asp His Ala Tyr Pro Pro Ser Ser Phe Thr 
        35                  40                  45              


Ile Pro Leu Ser Lys Asp Glu Glu Leu Ala Glu Ala Gly Pro Ser Arg 
    50                  55                  60                  


Pro Gly Ser Ser Ala Ile Ser Arg Arg Pro Val Leu Ser Arg Arg Arg 
65                  70                  75                  80  


Thr Ser Lys Lys Glu Tyr Val His Pro Tyr Leu Ser Gly Asn Phe Ala 
                85                  90                  95      


Pro Val Thr Thr Glu Cys Pro Leu Thr Asp Cys Leu Phe Glu Gly Thr 
            100                 105                 110         


Ile Pro Glu Glu Phe Ala Gly Ser Gln Tyr Val Arg Asn Gly Gly Asn 
        115                 120                 125             


Pro Leu Ala Asn Ser Glu Arg Asp Arg Asp Ala His Trp Phe Asp Ala 
    130                 135                 140                 


Asp Gly Met Leu Ala Gly Val Leu Phe Arg Arg Thr Pro Lys Gly Thr 
145                 150                 155                 160 


Ile Gln Pro Cys Phe Leu Asn Arg Phe Ile Leu Thr Asp Leu Leu Leu 
                165                 170                 175     


Ser Thr Pro Glu His Ser Arg Leu Pro Tyr Val Pro Ser Ile Ala Thr 
            180                 185                 190         


Leu Val Asn Pro His Thr Ser Val Phe Trp Leu Leu Cys Glu Ile Ile 
        195                 200                 205             


Arg Thr Phe Val Leu Ala Met Leu Thr Trp Leu Pro Gly Leu Gly Leu 
    210                 215                 220                 


Gly Gly Asn Gln Lys Leu Lys Arg Ile Ser Val Ala Asn Thr Ser Val 
225                 230                 235                 240 


Phe Trp His Asp Gly Lys Ala Met Ala Gly Cys Glu Ser Gly Pro Pro 
                245                 250                 255     


Met Arg Ile Met Leu Pro Gly Leu Glu Thr Ala Gly Trp Tyr Thr Gly 
            260                 265                 270         


Glu Glu Asp Lys Glu Lys Glu Thr Cys Asp Lys Asn Ser Gly Asn Ser 
        275                 280                 285             


Leu Thr Ser Ser Ser Ser Lys Gly Phe Gly Gly Gly Pro Pro Ile Val 
    290                 295                 300                 


Ser Met Leu Arg Glu Phe Thr Thr Ala His Pro Lys Ile Asp Pro Arg 
305                 310                 315                 320 


Thr Gln Glu Leu Leu Leu Tyr His Met Cys Phe Glu Pro Pro Tyr Leu 
                325                 330                 335     


Arg Ile Ser Val Ile Pro Ala Ser Gln Ser Lys Lys Thr Asp Leu Pro 
            340                 345                 350         


Ala His Ala Lys Thr Ile Lys Gly Lys Ala Val Arg Gly Leu Lys Gln 
        355                 360                 365             


Pro Lys Met Met His Asp Phe Gly Ala Thr Ala Thr Gln Thr Val Ile 
    370                 375                 380                 


Ile Asp Val Pro Leu Ser Leu Asp Met Met Asn Leu Val Arg Gly Lys 
385                 390                 395                 400 


Pro Ile Leu His Tyr Asp Pro Ser Gln Pro Thr Arg Phe Gly Ile Leu 
                405                 410                 415     


Pro Arg Tyr Glu Pro Glu Arg Val Arg Trp Tyr Glu Ser Ala Glu Ala 
            420                 425                 430         


Cys Cys Ile Tyr His Thr Ala Asn Ser Trp Asp Asp Asp Gly Lys Phe 
        435                 440                 445             


Asp Ala Ser His Glu His Ala Thr Arg Ser Ala Ile Arg Gly Val Asn 
    450                 455                 460                 


Met Leu Gly Cys Arg Leu Asn Ser Ala Thr Leu Val Tyr Ser Ala Gly 
465                 470                 475                 480 


Asn Leu Leu Pro Pro Ser His Val Leu Pro Pro Pro Asn Cys Pro Glu 
                485                 490                 495     


Lys Cys Gln Leu Tyr Tyr Trp Arg Phe Asp Leu Glu His Ala Glu Thr 
            500                 505                 510         


Asn Thr Ile Ser His Glu Phe Ala Leu Ser Asp Ile Pro Phe Glu Phe 
        515                 520                 525             


Pro Thr Ile Asn Glu Asp Tyr Ser Met Gln Gln Ala Cys Tyr Val Tyr 
    530                 535                 540                 


Gly Thr Ser Met Arg Asp Gly Thr Phe Asp Ala Gly Leu Gly Lys Ala 
545                 550                 555                 560 


Ala Lys Ile Asp Ala Leu Val Lys Leu Asp Ala Gln Ala Leu Ile Arg 
                565                 570                 575     


Lys Gly Lys Ala Met Trp Ser Gln Gly Arg Leu Lys Ala Gly Asp Ser 
            580                 585                 590         


Val Asp Thr Arg Thr Val Glu Glu Val Leu Thr Ala Gln Arg Asp Gly 
        595                 600                 605             


Ser Ala Ser Pro Glu Asp Pro Ile Lys Ile Phe Glu Met Pro Arg Gly 
    610                 615                 620                 


Trp Tyr Ala Gln Glu Thr Thr Phe Val Pro Arg Arg Ser Ser Thr Asn 
625                 630                 635                 640 


Glu Thr Ser Gln Glu Asp Asp Gly Trp Leu Val Cys Tyr Val Phe Asp 
                645                 650                 655     


Glu Ala Thr Gly Leu His Pro Ser Thr Gly Glu Val Leu Pro Gly Ala 
            660                 665                 670         


Ser Ser Glu Leu Trp Ile Ile Asp Ala Lys Leu Met Ser Arg Val Val 
        675                 680                 685             


Cys Arg Ile Lys Leu Pro Gln Arg Val Pro Tyr Gly Leu His Gly Thr 
    690                 695                 700                 


Leu Phe Thr Glu Glu Gln Ile Ala Ser Gln Lys Pro Ile Asp Pro Ser 
705                 710                 715                 720 


Gln Val Arg Ser Trp Ala Leu Ser Ile Asn Leu Ala Asp Pro Phe Ser 
                725                 730                 735     


Ser Ser Ala Leu Gly Ser Thr Val Tyr Ser Ala Ala Gly Lys Ala Ala 
            740                 745                 750         


Thr Ser Lys Phe Lys Asn Arg Glu Glu Thr Tyr Ala Ala Phe Ile Lys 
        755                 760                 765             


Asp Pro Ile Arg Ile Gly Ala Trp Trp Val Lys Arg Asn Ile Glu Leu 
    770                 775                 780                 


Leu Ile Ala 
785         


<210>  2
<211>  2364
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yarrowia codon-optimized UmCCO1

<400>  2
atggttaagg gctcctctaa ccgacgacag cactccgctt cccttcaggg actcccttct       60

tctcagcact gtgcccccgt tatctctatt ccttctcccc ctccccctgc tgaggatcac      120

gcttaccccc cttcctcttt cactattcct ctctccaagg atgaggagct tgctgaggcc      180

ggaccctctc gacccggttc ctctgctatt tctcgacgac ctgttctgtc tcgacgacga      240

acttctaaga aggagtacgt tcacccctac ctctccggca actttgcccc tgttaccact      300

gagtgccctc tcaccgattg tctctttgag ggtactatcc ctgaggagtt tgctggctcc      360

cagtacgtcc gaaacggcgg aaaccccctt gccaactccg agcgagatcg agatgcccac      420

tggttcgatg ctgacggtat gctggctgga gttctctttc gacgaacccc caagggcacc      480

attcagcctt gtttcctcaa ccgattcatt ctcaccgacc tcctgctctc tacccctgag      540

cactctcgac tcccttacgt cccttccatc gctactctcg tcaaccccca cacttccgtc      600

ttttggctcc tttgtgagat catccgaact ttcgttctgg ctatgcttac ctggctccct      660

ggcctcggac tcggtggcaa ccagaagctc aagcgaatct ctgttgctaa cacctccgtt      720

ttctggcacg acggaaaggc tatggctgga tgtgagtctg gaccccctat gcgaatcatg      780

ctccctggtc ttgagactgc cggctggtac actggtgagg aggataagga gaaggagact      840

tgtgataaga actctggcaa ctctctcact tcttcctctt ctaagggttt tggcggaggc      900

cctcccattg tctccatgct tcgagagttt accactgctc accccaagat tgaccctcga      960

acccaggagc tccttctcta ccacatgtgc ttcgagcccc cttaccttcg aatctctgtc     1020

atccctgctt ctcagtctaa gaagactgac ctccctgctc acgctaagac cattaagggt     1080

aaggctgtgc gaggtcttaa gcagcccaag atgatgcacg atttcggcgc taccgccact     1140

cagaccgtca tcatcgacgt ccctctctcc ctcgacatga tgaacctcgt ccgaggcaag     1200

cccattctgc actacgatcc ctctcagcct acccgattcg gtattcttcc ccgatacgag     1260

cctgagcgag tgcgatggta cgagtctgcc gaggcttgct gtatctacca caccgccaac     1320

tcttgggatg acgatggcaa gtttgacgct tctcacgagc acgctacccg atccgccatc     1380

cgaggcgtca acatgctcgg ctgccgactc aactctgcca ccctcgtgta ctctgctgga     1440

aaccttctcc ctccctctca cgtccttccc cctcccaact gccctgagaa gtgtcagctc     1500

tactactggc gattcgacct tgagcacgct gagactaaca ccatttccca cgagtttgct     1560

ctgtccgaca ttcctttcga gttccccacc atcaacgagg actactctat gcagcaggct     1620

tgttacgttt acggtacttc catgcgagat ggcacctttg acgctggact cggaaaggct     1680

gctaagattg acgcccttgt taagctggac gctcaggccc ttattcgaaa gggcaaggcc     1740

atgtggtccc agggacgact taaggctgga gactctgtgg acacccgaac cgttgaggag     1800

gttctcactg ctcagcgaga tggttctgcc tcccctgagg accctatcaa gattttcgag     1860

atgccccgag gatggtacgc tcaggagact accttcgtcc ctcgacgatc ctctactaac     1920

gagacttctc aggaggatga cggttggctc gtctgctacg tgttcgatga ggccactggc     1980

cttcaccctt ccaccggaga ggttctccct ggcgcttcct ccgagctgtg gatcattgat     2040

gccaagctca tgtcccgagt cgtttgccga atcaagctcc cccagcgagt cccttacgga     2100

ctccacggca ctctctttac cgaggagcag attgcctctc agaagcctat cgacccttct     2160

caggtccgat cctgggctct gtctatcaac cttgccgatc ccttctcctc ttccgccctt     2220

ggctctaccg tgtactccgc cgctggtaag gctgccacct ccaagtttaa gaaccgagag     2280

gagacttacg ctgccttcat caaggaccct atccgaatcg gcgcttggtg ggtcaagcga     2340

aacatcgagc tcctgattgc ttaa                                            2364


<210>  3
<211>  696
<212>  PRT
<213>  Fusarium fujikuroi

<400>  3

Met Lys Phe Leu Gln Gln Asn Ser Phe Thr Gln Thr Ser Met Ser Gln 
1               5                   10                  15      


Pro His Glu Asp Val Ser Pro Ala Ile Arg His Pro Tyr Leu Thr Gly 
            20                  25                  30          


Asn Phe Ala Pro Ile His Lys Thr Thr Asn Leu Thr Pro Cys Thr Tyr 
        35                  40                  45              


Ser Gly Cys Ile Pro Pro Glu Leu Thr Gly Gly Gln Tyr Val Arg Asn 
    50                  55                  60                  


Gly Gly Asn Pro Val Ser His Gln Asp Leu Gly Lys Asp Ala His Trp 
65                  70                  75                  80  


Phe Asp Gly Asp Gly Met Leu Ser Gly Val Ala Phe Arg Lys Ala Ser 
                85                  90                  95      


Ile Asp Gly Lys Thr Ile Pro Glu Phe Val Asn Gln Tyr Ile Leu Thr 
            100                 105                 110         


Asp Leu Tyr Leu Ser Arg Lys Thr Thr Ser Ile Ala Ser Pro Ile Met 
        115                 120                 125             


Pro Ser Ile Thr Thr Leu Val Asn Pro Leu Ser Thr Met Phe Gln Ile 
    130                 135                 140                 


Met Phe Ala Thr Phe Arg Thr Ile Phe Leu Val Ile Leu Ser Asn Leu 
145                 150                 155                 160 


Pro Gly Ser Gln Gln Ala Ile Lys Arg Ile Ser Val Ala Asn Thr Ala 
                165                 170                 175     


Val Leu Tyr His Asp Gly Arg Ala Leu Ala Thr Cys Glu Ser Gly Pro 
            180                 185                 190         


Pro Met Arg Ile Gln Leu Pro Ser Leu Asp Thr Val Gly Trp Phe Asp 
        195                 200                 205             


Gly Val Glu Ala Glu Gly Glu Pro Glu Ile Ser Gln Ala Gly Ser Asp 
    210                 215                 220                 


Asp Ser Pro Phe Gly Gly Ser Gly Ile Phe Ser Phe Met Lys Glu Trp 
225                 230                 235                 240 


Thr Thr Gly His Pro Lys Val Asp Pro Val Thr Gly Glu Met Leu Leu 
                245                 250                 255     


Tyr His Asn Thr Phe Met Pro Pro Tyr Val His Cys Ser Val Leu Pro 
            260                 265                 270         


Lys Ser Asn Glu Lys Ala Pro Gly His Arg Leu Val Asn Gln Pro Val 
        275                 280                 285             


Leu Gly Val Ser Gly Ala Arg Met Met His Asp Phe Gly Ala Ser Arg 
    290                 295                 300                 


Ser His Thr Ile Ile Met Asp Leu Pro Leu Ser Leu Asp Pro Leu Asn 
305                 310                 315                 320 


Thr Met Lys Gly Lys Glu Val Val Ala Tyr Asp Pro Thr Lys Pro Ser 
                325                 330                 335     


Arg Phe Gly Val Phe Pro Arg His Leu Pro Ser Ser Val Arg Trp Phe 
            340                 345                 350         


His Thr Ala Pro Cys Cys Ile Phe His Thr Ala Asn Thr Trp Asp Ser 
        355                 360                 365             


Gln Ser Ser Glu Gly Glu Leu Ser Val Asn Leu Leu Ala Cys Arg Met 
    370                 375                 380                 


Thr Ser Ser Thr Leu Val Tyr Thr Ala Gly Asn Ile Arg Pro Pro Val 
385                 390                 395                 400 


Arg Ser Arg Cys Thr Gln Ala Arg Val Trp Ser Asp Glu Arg Glu Glu 
                405                 410                 415     


Thr Ala Cys Arg Tyr Lys Glu Ala Pro Ala Leu Glu Ser Pro Gly Glu 
            420                 425                 430         


Ser Thr Gly Leu Ala Asp Tyr Phe Pro Ile Thr Ala Glu Ser Asp Asp 
        435                 440                 445             


Tyr Asp Gln Cys Arg Leu Tyr Tyr Tyr Glu Phe Asp Leu Ala Met Glu 
    450                 455                 460                 


Ser Arg Asn His Val Lys Ser Gln Trp Ala Leu Ser Ala Ile Pro Phe 
465                 470                 475                 480 


Glu Phe Pro Ser Val Arg Pro Asp Arg Glu Met Gln Glu Ala Arg Tyr 
                485                 490                 495     


Ile Tyr Gly Cys Ser Thr Ser Thr Ser Cys Phe Gly Val Ala Leu Gly 
            500                 505                 510         


Arg Ala Asp Lys Val Asp Leu Leu Val Lys Met Asp Ala Lys Thr Leu 
        515                 520                 525             


Ile Gln Arg Gly Lys Lys Met Asn Ala Thr Ser Ile Thr Gly Cys Val 
    530                 535                 540                 


Asp Arg Arg Ser Val Cys Glu Ile Leu Gln Glu Gln Arg Lys Asp Asp 
545                 550                 555                 560 


Pro Ile Tyr Ile Phe Arg Leu Pro Pro Asn His Tyr Ala Gln Glu Pro 
                565                 570                 575     


Arg Phe Val Pro Arg Ala Cys Ser Thr Glu Glu Asp Asp Gly Tyr Leu 
            580                 585                 590         


Leu Phe Tyr Val Phe Asp Glu Ser Gln Leu Leu Pro Ser Gly Asp Cys 
        595                 600                 605             


Pro Pro Ser Ala Thr Ser Glu Leu Trp Ile Leu Asp Ala Lys Asn Met 
    610                 615                 620                 


Arg Asp Val Val Ala Lys Val Arg Leu Pro Gln Arg Val Pro Tyr Gly 
625                 630                 635                 640 


Leu His Gly Thr Trp Phe Ser Ser Gln Asp Ile Glu Ser Gln Arg Ser 
                645                 650                 655     


Val Glu Ser Leu Arg Ser Leu Glu Val Val Gln Arg Lys Lys Glu Glu 
            660                 665                 670         


Trp Val Asn Ser Gly Gly Gln Ile Arg Lys Ser Trp Met Val Leu Arg 
        675                 680                 685             


Glu Lys Leu Glu Lys Ala Val Gly 
    690                 695     


<210>  4
<211>  2091
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yarrowia codon-optimized FfCarX

<400>  4
atgaagtttc tccagcagaa ctcctttacc cagacctcta tgtctcagcc tcacgaggat       60

gtctctcccg ccattcgaca cccttacctt accggcaact ttgctcctat tcacaagacc      120

actaacctca ctccctgtac ttactctggc tgcattcccc ccgagcttac cggaggtcag      180

tacgttcgaa acggcggaaa ccctgtctcc caccaggatc tcggaaagga tgctcactgg      240

ttcgatggcg acggtatgct ctctggcgtc gcctttcgaa aggcttccat tgatggcaag      300

actatccctg agttcgttaa ccagtacatt cttaccgacc tttacctttc tcgaaagacc      360

acctctattg cttcccctat tatgccctct atcaccaccc tggttaaccc tctctctact      420

atgtttcaga tcatgttcgc caccttccga actatcttcc tcgtcattct ctccaacctc      480

cctggttctc agcaggctat caagcgaatc tccgttgcca acactgctgt tctttaccac      540

gatggtcgag ctcttgccac ttgcgagtct ggccccccca tgcgaatcca gcttccctcc      600

ctcgataccg ttggctggtt cgacggtgtt gaggctgagg gtgagcctga gatttctcag      660

gccggctctg atgactctcc cttcggcggt tccggcatct tctcctttat gaaggagtgg      720

accaccggcc accctaaggt ggaccccgtt accggagaga tgcttctcta ccacaacacc      780

ttcatgcctc cctacgtgca ctgctctgtt cttcccaagt ctaacgagaa ggctcccgga      840

caccgacttg ttaaccagcc cgttcttggt gtttctggtg cccgaatgat gcacgacttc      900

ggagcctctc gatctcacac tatcatcatg gaccttcccc tgtctctgga ccctctcaac      960

actatgaagg gaaaggaggt tgttgcttac gaccctacca agccttctcg attcggtgtg     1020

ttcccccgac accttccctc ttccgtgcga tggtttcaca ctgctccttg ctgtatcttt     1080

cacactgcta acacttggga ttctcagtcc tctgagggag agctttctgt taacctcctt     1140

gcctgccgaa tgacctcttc tacccttgtt tacactgccg gcaacatccg acctcccgtt     1200

cgatctcgat gtactcaggc ccgagtctgg tccgatgagc gagaggagac tgcttgtcga     1260

tacaaggagg ctcctgctct tgagtctcct ggtgagtcca ctggccttgc cgactacttt     1320

cccattaccg ctgagtccga cgactacgat cagtgccgac tctactacta cgagtttgac     1380

cttgctatgg agtcccgaaa ccacgtcaag tcccagtggg ctctctctgc cattcctttc     1440

gagtttccct ctgtgcgacc tgaccgagag atgcaggagg ctcgatacat ctacggctgt     1500

tccacttcca cttcttgctt cggtgtggct ctcggacgag ctgataaggt tgaccttctc     1560

gttaagatgg atgccaagac cctcattcag cgaggaaaga agatgaacgc tacttccatc     1620

accggatgcg ttgatcgacg atctgtctgc gagatccttc aggagcagcg aaaggatgac     1680

cctatttaca ttttccgact tccccctaac cactacgctc aggagccccg attcgttccc     1740

cgagcttgtt ctactgagga ggacgacgga tacctccttt tctacgtgtt cgacgagtct     1800

cagctccttc cctctggcga ttgtcctccc tctgctactt ctgagctttg gattcttgac     1860

gctaagaaca tgcgagatgt tgtggccaag gtccgacttc cccagcgagt tccttacggt     1920

ctgcacggta cttggttctc ttctcaggat attgagtctc agcgatctgt ggagtctctt     1980

cgatctcttg aggttgtgca gcgaaagaag gaggagtggg ttaactctgg aggccagatt     2040

cgaaagtcct ggatggttct tcgagagaag ctggagaagg ctgttggata g              2091


<210>  5
<211>  369
<212>  PRT
<213>  Crocus sativus

<400>  5

Met Gln Val Asp Pro Thr Lys Gly Ile Gly Leu Ala Asn Thr Ser Leu 
1               5                   10                  15      


Gln Phe Ser Asn Gly Arg Leu His Ala Leu Cys Glu Tyr Asp Leu Pro 
            20                  25                  30          


Tyr Val Val Arg Leu Ser Pro Glu Asp Gly Asp Ile Ser Thr Val Gly 
        35                  40                  45              


Arg Ile Glu Asn Asn Val Ser Thr Lys Ser Thr Thr Ala His Pro Lys 
    50                  55                  60                  


Thr Asp Pro Val Thr Gly Glu Thr Phe Ser Phe Ser Tyr Gly Pro Ile 
65                  70                  75                  80  


Gln Pro Tyr Val Thr Tyr Ser Arg Tyr Asp Cys Asp Gly Lys Lys Ser 
                85                  90                  95      


Gly Pro Asp Val Pro Ile Phe Ser Phe Lys Glu Pro Ser Phe Val His 
            100                 105                 110         


Asp Phe Ala Ile Thr Glu His Tyr Ala Val Phe Pro Asp Ile Gln Ile 
        115                 120                 125             


Val Met Lys Pro Ala Glu Ile Val Arg Gly Arg Arg Met Ile Gly Pro 
    130                 135                 140                 


Asp Leu Glu Lys Val Pro Arg Leu Gly Leu Leu Pro Arg Tyr Ala Thr 
145                 150                 155                 160 


Ser Asp Ser Glu Met Arg Trp Phe Asp Val Pro Gly Phe Asn Met Val 
                165                 170                 175     


His Val Val Asn Ala Trp Glu Glu Glu Gly Gly Glu Val Val Val Ile 
            180                 185                 190         


Val Ala Pro Asn Val Ser Pro Ile Glu Asn Ala Ile Asp Arg Phe Asp 
        195                 200                 205             


Leu Leu His Val Ser Val Glu Met Ala Arg Ile Glu Leu Lys Ser Gly 
    210                 215                 220                 


Ser Val Ser Arg Thr Leu Leu Ser Ala Glu Asn Leu Asp Phe Gly Val 
225                 230                 235                 240 


Ile His Arg Gly Tyr Ser Gly Arg Lys Ser Arg Tyr Ala Tyr Leu Gly 
                245                 250                 255     


Val Gly Asp Pro Met Pro Lys Ile Arg Gly Val Val Lys Val Asp Phe 
            260                 265                 270         


Glu Leu Ala Gly Arg Gly Glu Cys Val Val Ala Arg Arg Glu Phe Gly 
        275                 280                 285             


Val Gly Cys Phe Gly Gly Glu Pro Phe Phe Val Pro Ala Ser Ser Lys 
    290                 295                 300                 


Lys Ser Gly Gly Glu Glu Asp Asp Gly Tyr Val Val Ser Tyr Leu His 
305                 310                 315                 320 


Asp Glu Gly Lys Gly Glu Ser Ser Phe Val Val Met Asp Ala Arg Ser 
                325                 330                 335     


Pro Glu Leu Glu Ile Leu Ala Glu Val Val Leu Pro Arg Arg Val Pro 
            340                 345                 350         


Tyr Gly Phe His Gly Leu Phe Val Thr Glu Ala Glu Leu Leu Ser Gln 
        355                 360                 365             


Gln 
    


<210>  6
<211>  1110
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yarrowia codon-optimized CsZCO

<400>  6
atgcaggtgg accccaccaa gggtatcggc ctggccaaca cttctctcca gttctccaac       60

ggacgactcc acgctctttg cgagtacgac ctcccctacg tcgttcgact ctcccccgag      120

gacggtgaca tctctaccgt cggacgaatc gagaacaacg tttctactaa gtctaccacc      180

gcccacccca agaccgaccc cgtcaccgga gagaccttct ctttctccta cggtcccatt      240

cagccctacg tcacctactc ccgatacgac tgcgacggca agaagtccgg ccccgacgtg      300

cccatcttct ctttcaagga gccctctttc gtccacgact tcgccatcac cgagcactac      360

gccgtctttc ccgacattca gatcgtgatg aagcccgccg agatcgttcg aggacgacga      420

atgatcggcc ccgaccttga gaaggtcccc cgactgggcc ttctcccccg atacgccacc      480

tccgactccg agatgcgatg gttcgacgtg cccggtttca acatggttca cgtggttaac      540

gcttgggagg aggagggcgg agaggtcgtg gtcatcgtgg cccccaacgt gtcccccatt      600

gagaacgcca tcgaccgatt cgacctcctc cacgtgtctg tggagatggc ccgaatcgag      660

ctgaagtccg gttccgtgtc ccgaaccctt ctctctgccg agaacctcga tttcggtgtg      720

attcaccgag gctactccgg tcgaaagtcc cgatacgctt acctcggagt cggcgacccc      780

atgcccaaga ttcgaggtgt ggtcaaggtg gacttcgagc tggccggacg aggagagtgc      840

gtggttgccc gacgagagtt cggcgtgggt tgtttcggtg gagagccctt ctttgtcccc      900

gcttcttcca agaagtctgg aggcgaggag gacgatggct acgttgtgtc ttaccttcac      960

gacgagggaa agggagagtc ctctttcgtc gtgatggacg ctcgatctcc cgagctggag     1020

attcttgccg aggtggttct gccccgacga gttccctacg gttttcacgg cctctttgtt     1080

accgaggccg agcttctctc ccagcagtag                                      1110


<210>  7
<211>  620
<212>  PRT
<213>  Drosophila melanogaster

<400>  7

Met Ala Ala Gly Val Phe Lys Ser Phe Met Arg Asp Phe Phe Ala Val 
1               5                   10                  15      


Lys Tyr Asp Glu Gln Arg Asn Asp Pro Gln Ala Glu Arg Leu Asp Gly 
            20                  25                  30          


Asn Gly Arg Leu Tyr Pro Asn Cys Ser Ser Asp Val Trp Leu Arg Ser 
        35                  40                  45              


Cys Glu Arg Glu Ile Val Asp Pro Ile Glu Gly His His Ser Gly His 
    50                  55                  60                  


Ile Pro Lys Trp Ile Cys Gly Ser Leu Leu Arg Asn Gly Pro Gly Ser 
65                  70                  75                  80  


Trp Lys Val Gly Asp Met Thr Phe Gly His Leu Phe Asp Cys Ser Ala 
                85                  90                  95      


Leu Leu His Arg Phe Ala Ile Arg Asn Gly Arg Val Thr Tyr Gln Asn 
            100                 105                 110         


Arg Phe Val Asp Thr Glu Thr Leu Arg Lys Asn Arg Ser Ala Gln Arg 
        115                 120                 125             


Ile Val Val Thr Glu Phe Gly Thr Ala Ala Val Pro Asp Pro Cys His 
    130                 135                 140                 


Ser Ile Phe Asp Arg Phe Ala Ala Ile Phe Arg Pro Asp Ser Gly Thr 
145                 150                 155                 160 


Asp Asn Ser Met Ile Ser Ile Tyr Pro Phe Gly Asp Gln Tyr Tyr Thr 
                165                 170                 175     


Phe Thr Glu Thr Pro Phe Met His Arg Ile Asn Pro Cys Thr Leu Ala 
            180                 185                 190         


Thr Glu Ala Arg Ile Cys Thr Thr Asp Phe Val Gly Val Val Asn His 
        195                 200                 205             


Thr Ser His Pro His Val Leu Pro Ser Gly Thr Val Tyr Asn Leu Gly 
    210                 215                 220                 


Thr Thr Met Thr Arg Ser Gly Pro Ala Tyr Thr Ile Leu Ser Phe Pro 
225                 230                 235                 240 


His Gly Glu Gln Met Phe Glu Asp Ala His Val Val Ala Thr Leu Pro 
                245                 250                 255     


Cys Arg Trp Lys Leu His Pro Gly Tyr Met His Thr Phe Gly Leu Thr 
            260                 265                 270         


Asp His Tyr Phe Val Ile Val Glu Gln Pro Leu Ser Val Ser Leu Thr 
        275                 280                 285             


Glu Tyr Ile Lys Ala Gln Leu Gly Gly Gln Asn Leu Ser Ala Cys Leu 
    290                 295                 300                 


Lys Trp Phe Glu Asp Arg Pro Thr Leu Phe His Leu Ile Asp Arg Val 
305                 310                 315                 320 


Ser Gly Lys Leu Val Gln Thr Tyr Glu Ser Glu Ala Phe Phe Tyr Leu 
                325                 330                 335     


His Ile Ile Asn Cys Phe Glu Arg Asp Gly His Val Val Val Asp Ile 
            340                 345                 350         


Cys Ser Tyr Arg Asn Pro Glu Met Ile Asn Cys Met Tyr Leu Glu Ala 
        355                 360                 365             


Ile Ala Asn Met Gln Thr Asn Pro Asn Tyr Ala Thr Leu Phe Arg Gly 
    370                 375                 380                 


Arg Pro Leu Arg Phe Val Leu Pro Leu Gly Thr Ile Pro Pro Ala Ser 
385                 390                 395                 400 


Ile Ala Lys Arg Gly Leu Val Lys Ser Phe Ser Leu Ala Gly Leu Ser 
                405                 410                 415     


Ala Pro Gln Val Ser Arg Thr Met Lys His Ser Val Ser Gln Tyr Ala 
            420                 425                 430         


Asp Ile Thr Tyr Met Pro Thr Asn Gly Lys Gln Ala Thr Ala Gly Glu 
        435                 440                 445             


Glu Ser Pro Lys Arg Asp Ala Lys Arg Gly Arg Tyr Glu Glu Glu Asn 
    450                 455                 460                 


Leu Val Asn Leu Val Thr Met Glu Gly Ser Gln Ala Glu Ala Phe Gln 
465                 470                 475                 480 


Gly Thr Asn Gly Ile Gln Leu Arg Pro Glu Met Leu Cys Asp Trp Gly 
                485                 490                 495     


Cys Glu Thr Pro Arg Ile Tyr Tyr Glu Arg Tyr Met Gly Lys Asn Tyr 
            500                 505                 510         


Arg Tyr Phe Tyr Ala Ile Ser Ser Asp Val Asp Ala Val Asn Pro Gly 
        515                 520                 525             


Thr Leu Ile Lys Val Asp Val Trp Asn Lys Ser Cys Leu Thr Trp Cys 
    530                 535                 540                 


Glu Glu Asn Val Tyr Pro Ser Glu Pro Ile Phe Val Pro Ser Pro Asp 
545                 550                 555                 560 


Pro Lys Ser Glu Asp Asp Gly Val Ile Leu Ala Ser Met Val Leu Gly 
                565                 570                 575     


Gly Leu Asn Asp Arg Tyr Val Gly Leu Ile Val Leu Cys Ala Lys Thr 
            580                 585                 590         


Met Thr Glu Leu Gly Arg Cys Asp Phe His Thr Asn Gly Pro Val Pro 
        595                 600                 605             


Lys Cys Leu His Gly Trp Phe Ala Pro Asn Ala Ile 
    610                 615                 620 


<210>  8
<211>  1863
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yarrowia codon-optimized DmNinaB

<400>  8
atggccgctg gtgttttcaa gtcttttatg cgagatttct ttgctgttaa gtacgatgag       60

cagcgaaacg acccccaggc cgagcgactg gacggcaacg gacgactgta ccccaactgc      120

tcctctgatg tttggcttcg atcttgcgag cgagagatcg ttgaccccat tgagggccac      180

cactccggtc acattcccaa gtggatttgc ggttccctgc tccgaaacgg ccccggctct      240

tggaaggttg gcgacatgac cttcggccac ctgttcgact gctccgccct gctccaccga      300

tttgccattc gaaacggacg agtcacctac cagaaccgat ttgttgacac tgagactctg      360

cgaaagaacc gatctgccca gcgaattgtt gtcaccgagt ttggcactgc cgctgttccc      420

gatccctgtc actccatctt cgaccgattt gccgccattt ttcgacccga ttctggaacc      480

gataactcca tgatttccat ctaccccttc ggcgaccagt actacacttt caccgagact      540

ccctttatgc accgaattaa cccctgcact ctcgctactg aggctcgaat ctgcaccacc      600

gacttcgttg gcgttgtcaa ccacacttct cacccccacg ttcttccctc tggcactgtt      660

tacaacctgg gcaccactat gacccgatct ggacccgctt acactatcct ctctttcccc      720

cacggcgagc agatgttcga ggacgctcac gttgtcgcca ctctgccctg ccgatggaag      780

ctgcaccccg gttatatgca caccttcggc ctcactgacc actactttgt cattgttgag      840

cagccccttt ccgtttccct cactgagtac atcaaggccc agcttggcgg acagaacctt      900

tccgcttgcc tcaagtggtt cgaggaccga cccactctct ttcaccttat tgatcgagtt      960

tccggcaagc tggtccagac ctacgagtcc gaggctttct tctacctgca catcatcaac     1020

tgctttgagc gagatggcca cgttgtcgtt gacatttgct cttaccgaaa ccccgagatg     1080

attaactgca tgtacctgga ggccattgcc aacatgcaga ctaaccccaa ctacgctacc     1140

ctctttcgag gacgacccct tcgattcgtc ctgcccctcg gcactattcc ccccgcctct     1200

atcgccaagc gaggactcgt caagtccttc tccctcgctg gactctccgc tccccaggtt     1260

tctcgaacca tgaagcactc cgtttctcag tacgccgata ttacctacat gcccaccaac     1320

ggaaagcagg ccactgctgg agaggagtcc cccaagcgag atgccaagcg aggccgatac     1380

gaggaggaga accttgtcaa cctggttact atggagggct ctcaggccga ggcttttcag     1440

ggcaccaacg gcattcagct tcgacccgag atgctgtgtg attggggctg tgagactccc     1500

cgaatctact acgagcgata catgggcaag aactaccgat acttctacgc catttcttcc     1560

gatgttgatg ctgtcaaccc cggcaccctc atcaaggttg atgtctggaa caagtcttgt     1620

cttacctggt gcgaggagaa cgtctacccc tctgagccca tttttgtccc ctctcccgat     1680

cccaagtccg aggacgatgg cgttatcctg gcctctatgg ttcttggcgg tcttaacgac     1740

cgatacgtcg gccttattgt tctttgtgcc aagaccatga ccgagctggg ccgatgtgat     1800

ttccacacca acggacccgt tcccaagtgc ctccacggtt ggtttgctcc caacgccatt     1860

tag                                                                   1863


<210>  9
<211>  525
<212>  PRT
<213>  Danio rerio

<400>  9

Met Leu Ser Phe Phe Trp Arg Asn Gly Ile Glu Thr Pro Glu Pro Leu 
1               5                   10                  15      


Lys Ala Asp Val Ser Gly Ser Ile Pro Pro Trp Leu Gln Gly Thr Leu 
            20                  25                  30          


Leu Arg Asn Gly Pro Gly Leu Phe Ser Val Gly Asn Thr Ser Tyr Lys 
        35                  40                  45              


His Trp Phe Asp Gly Met Ala Leu Ile His Ser Phe Thr Phe Lys Asp 
    50                  55                  60                  


Gly Glu Val Phe Tyr Arg Ser Lys Tyr Leu Lys Ser Glu Thr Tyr Lys 
65                  70                  75                  80  


Lys Asn Ile Ala Ala Asp Arg Ile Val Val Ser Glu Phe Gly Thr Met 
                85                  90                  95      


Val Tyr Pro Asp Pro Cys Lys Asn Ile Phe Ser Arg Ala Phe Ser Tyr 
            100                 105                 110         


Met Met Asn Ala Ile Pro Asp Phe Thr Asp Asn Asn Leu Ile Asn Ile 
        115                 120                 125             


Ile Lys Tyr Gly Glu Asp Tyr Tyr Ala Ser Ser Glu Val Asn Tyr Ile 
    130                 135                 140                 


Asn Gln Ile Asp Pro Leu Thr Leu Glu Thr Leu Gly Arg Thr Asn Tyr 
145                 150                 155                 160 


Arg Asn His Ile Ala Ile Asn Leu Ala Thr Ala His Pro His Tyr Asp 
                165                 170                 175     


Glu Glu Gly Asn Thr Tyr Asn Met Gly Thr Ala Ile Met Asn Leu Gly 
            180                 185                 190         


Arg Pro Lys Tyr Val Ile Phe Lys Val Pro Ala Asn Thr Ser Asp Lys 
        195                 200                 205             


Glu Asn Lys Lys Pro Ala Leu Ser Glu Val Glu Gln Val Cys Ser Ile 
    210                 215                 220                 


Pro Ile Arg Pro Ser Leu Tyr Pro Ser Tyr Phe His Ser Phe Gly Met 
225                 230                 235                 240 


Thr Glu Asn Tyr Ile Ile Phe Val Glu Gln Ala Phe Lys Leu Asp Ile 
                245                 250                 255     


Val Lys Leu Ala Thr Ala Tyr Phe Arg Asp Ile Asn Trp Gly Ser Cys 
            260                 265                 270         


Leu Lys Phe Asp Gln Asp Asp Ile Asn Val Phe His Leu Val Asn Lys 
        275                 280                 285             


Lys Thr Gly Lys Ala Val Ser Val Lys Tyr Tyr Thr Asp Pro Phe Val 
    290                 295                 300                 


Thr Phe His His Ile Asn Ala Tyr Glu Asp Asp Gly His Val Val Phe 
305                 310                 315                 320 


Asp Leu Ile Thr Tyr Lys Asp Ser Lys Leu Tyr Asp Met Phe Tyr Ile 
                325                 330                 335     


Gln Asn Met Lys Gln Asp Val Lys Arg Phe Ile Glu Thr Asn Lys Asp 
            340                 345                 350         


Phe Ala Gln Pro Val Cys Gln Arg Phe Val Leu Pro Val Asn Val Asp 
        355                 360                 365             


Lys Glu Thr Pro Gln Asp Ile Asn Leu Val Lys Leu Gln Asp Thr Thr 
    370                 375                 380                 


Ala Thr Ala Val Leu Lys Glu Asp Gly Ser Val Tyr Cys Thr Pro Asp 
385                 390                 395                 400 


Ile Ile Phe Lys Gly Leu Glu Leu Pro Ala Ile Asn Tyr Lys Phe Asn 
                405                 410                 415     


Ser Lys Lys Asn Arg Tyr Phe Tyr Gly Thr Arg Val Glu Trp Ser Pro 
            420                 425                 430         


Tyr Pro Asn Lys Val Ala Lys Val Asp Val Val Thr Arg Thr His Lys 
        435                 440                 445             


Ile Trp Thr Glu Glu Glu Cys Tyr Pro Ser Glu Pro Val Phe Ile Ala 
    450                 455                 460                 


Ser Pro Asp Ala Val Asp Glu Asp Asp Gly Val Ile Leu Ser Ser Val 
465                 470                 475                 480 


Val Ser Phe Asn Pro Gln Arg Pro Pro Phe Leu Val Val Leu Asp Ala 
                485                 490                 495     


Lys Ser Phe Lys Glu Ile Ala Arg Ala Thr Ile Asp Ala Ser Ile His 
            500                 505                 510         


Met Asp Leu His Gly Leu Phe Ile His Asp Lys Ser Thr 
        515                 520                 525 


<210>  10
<211>  1578
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yarrowia codon-optimized DrBCO

<400>  10
atgctctctt tcttctggcg aaacggtatc gagacccccg agcccctcaa ggctgacgtt       60

tccggctcta tccctccctg gcttcaggga acccttctcc gaaacggtcc tggtctgttc      120

tccgttggca acacttccta caagcactgg ttcgatggta tggctctcat tcactccttc      180

acctttaagg atggtgaggt tttttaccga tctaagtacc tgaagtctga gacttacaag      240

aagaacatcg ctgccgaccg aatcgttgtg tctgagttcg gaaccatggt gtaccccgat      300

ccctgcaaga acattttctc ccgagccttc tcttacatga tgaacgccat tcctgacttt      360

accgataaca acctcattaa catcattaag tacggtgagg attactacgc ctcctctgag      420

gtcaactaca tcaaccagat tgaccccctg acccttgaga ctctcggacg aactaactac      480

cgaaaccaca ttgccatcaa ccttgccact gctcaccctc actacgacga ggagggtaac      540

acttacaaca tgggcactgc tattatgaac ctcggtcgac ccaagtacgt gattttcaag      600

gtgcccgcca acacctctga taaggagaac aagaagcctg ccctctctga ggtggagcag      660

gtttgctcca ttcccatccg accctccctt tacccttctt acttccactc ttttggcatg      720

actgagaact acatcatctt cgttgagcag gccttcaagc tggacatcgt caagctggct      780

actgcttact tccgagatat taactgggga tcttgcctta agttcgacca ggatgacatt      840

aacgtgttcc acctggtcaa caagaagact ggtaaggctg tgtccgtgaa gtactacact      900

gacccctttg ttaccttcca ccacatcaac gcttacgagg acgatggcca cgtcgtcttc      960

gatctcatta cttacaagga ctctaagctg tacgatatgt tctacattca gaacatgaag     1020

caggacgtca agcgatttat tgagactaac aaggacttcg ctcagcccgt gtgccagcga     1080

tttgtccttc ccgtcaacgt tgataaggag acccctcagg acatcaacct tgtcaagctg     1140

caggacacca ctgccactgc tgtcctgaag gaggacggct ctgtctactg cacccctgac     1200

atcattttta agggtcttga gctccctgct atcaactaca agtttaactc taagaagaac     1260

cgatacttct acggcacccg agtggagtgg tccccttacc ctaacaaggt cgctaaggtg     1320

gacgttgtta ctcgaaccca caagatttgg actgaggagg agtgttaccc ttctgagcct     1380

gtctttattg cctcccctga cgccgttgat gaggatgacg gtgtgattct ttcttctgtg     1440

gtttctttca acccccagcg accccctttc ctggttgtcc tcgatgctaa gtccttcaag     1500

gagattgctc gagctaccat cgatgcctct attcacatgg accttcacgg ccttttcatc     1560

cacgacaagt ctacctaa                                                   1578


<210>  11
<211>  281
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Danio rerio BCO TPI aa

<400>  11

Lys Gln Lys Ser Asn His Ile Leu Gln Tyr Ser Pro Val Ile Thr Ala 
1               5                   10                  15      


Ser Ile Thr Pro Val Gln Val Ser Leu Gly Phe Leu Phe Thr Asp Thr 
            20                  25                  30          


Val Ile Tyr Leu Thr Ile Ser Leu Gln Val Thr Gln Lys Val His Val 
        35                  40                  45              


Gly Asn Glu Pro Gln Thr Lys Thr Arg Tyr Asp Lys Ile Ala Leu Phe 
    50                  55                  60                  


Asp Ala Glu Phe Asp Gly Val Ser Ile Gly Val Met Thr Phe Ile Cys 
65                  70                  75                  80  


Ile His Thr Lys Lys Ser Trp Trp Tyr Phe Cys Val Ile Thr Ser Asp 
                85                  90                  95      


Ile Tyr Ala Pro Pro Asn Pro Pro Ala Thr Val Lys Ser Val Ser Leu 
            100                 105                 110         


Leu Tyr Met Leu Thr Lys Pro Pro Thr Val Gln Arg Asn Pro Ser Ala 
        115                 120                 125             


Lys Ser His Asn Gln Leu Ile Thr Thr His Pro Met Thr Ser Pro Gln 
    130                 135                 140                 


Ile Leu Tyr Ala Phe Arg His Tyr Tyr Ser Ser Leu Gln Arg Arg Cys 
145                 150                 155                 160 


Leu Arg Phe His Phe Cys Ser Ile Thr Ser Leu Asn Pro Tyr Arg Gln 
                165                 170                 175     


Ile Arg Pro Trp His Val Ser Arg Leu Ile Ser Pro Arg Val Leu His 
            180                 185                 190         


Gln Gly Gly Gly Val Arg Asn Thr Val Arg Ala His Ser Lys Gly Val 
        195                 200                 205             


Arg Val Arg Ala Ser Asp Asn Ile Ala Trp Thr Arg Arg His Ile Leu 
    210                 215                 220                 


Asp Phe Trp Ala Arg Cys Ile His Leu Leu Arg Phe Pro Thr Leu Pro 
225                 230                 235                 240 


Pro Val Ser Pro Ser Gln Pro Ile Glu Gly Asn Leu Ile Arg Asp Thr 
                245                 250                 255     


Phe Val Ile His Ser Gln Ile Tyr Lys Gln Cys His Ser Pro Ser Tyr 
            260                 265                 270         


Ser Tyr Ile Gln His Asn Tyr Ile Gln 
        275                 280     


<210>  12
<211>  880
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yarrowia codon-optimized DrBCO-TPI

<400>  12
aaacaaaaga gctgaaatca tatccttcag tagtagtata gtcctgttat cacagcatca       60

attacccccg tccaagtaag ttgattggga tttttgttta cagatacagt aatatacttg      120

actatttctt tacaggtgac tcagaaagtg catgttggaa atgagccaca gaccaagaca      180

agatatgaca aaattgcact attcgatgca gaattcgacg gtgtttccat tggtgttatg      240

acattcatct gcattcatac aaaaaagtct tggtagtggt acttttgcgt tattacctcc      300

gatatctacg caccccccaa cccccctgct acagtaaaga gtgtgagtct actgtacatg      360

cttactaaac cacctactgt acagcgaaac ccctcagcaa aatcacacaa tcagctcatt      420

acaacacacc caatgacctc accacaaatt ctatacgcct tttgacgcca ttattacagt      480

agcttgcaac gccgttgtct taggttccat ttttagtgct ctattacctc acttaacccg      540

tataggcaga tcaggccatg gcactaagtg tagagctaga ggttgatatc gccacgagtg      600

ctccatcagg gctagggtgg ggttagaaat acagtccgtg cgcactcaaa aggcgtccgg      660

gttagggcat ccgataatat cgcctggact cggcgccata ttctcgactt ctgggcgcgt      720

tgtattcatc tcctccgctt cccaacactt ccacccgttt ctccatccca accaatagaa      780

tagggtaacc ttattcggga cactttcgtc atacatagtc agatatacaa gcaatgtcac      840

tctccttcgt actcgtacat acaacacaac tacattcaaa                            880


<210>  13
<211>  531
<212>  PRT
<213>  Ictalurus punctatus

<400>  13

Met Glu Ala Ile Phe Cys Arg Asn Gly Thr Glu Thr Pro Glu Pro Val 
1               5                   10                  15      


Lys Ala Val Val Ser Gly Ala Ile Pro Pro Trp Leu Gln Gly Thr Leu 
            20                  25                  30          


Leu Arg Asn Gly Pro Gly Leu Phe Ser Ile Gly Lys Thr Ser Tyr Asn 
        35                  40                  45              


His Trp Phe Asp Gly Leu Ser Leu Ile His Ser Phe Thr Phe Lys His 
    50                  55                  60                  


Gly Asp Val Tyr Tyr Arg Ser Lys Phe Leu Arg Ser Asp Thr Tyr Lys 
65                  70                  75                  80  


Lys Asn Ile Ala Ala Asn Arg Ile Val Val Ser Glu Phe Gly Thr Met 
                85                  90                  95      


Val Tyr Pro Asp Pro Cys Lys Asn Ile Phe Ser Lys Ala Phe Thr Tyr 
            100                 105                 110         


Leu Leu Asn Ser Ile Pro Asp Phe Thr Asp Asn Asn Leu Val Ser Ile 
        115                 120                 125             


Ile Lys Tyr Gly Asp Asp Tyr Tyr Thr Ser Ser Glu Ile Asn Tyr Ile 
    130                 135                 140                 


Asn Gln Ile Asn Pro Val Thr Leu Asp Thr Ile Gly Arg Ala Asn Tyr 
145                 150                 155                 160 


Arg Asn Tyr Ile Ser Leu Asn Leu Ala Thr Ala His Pro His Tyr Asp 
                165                 170                 175     


Asp Glu Gly Asn Thr Tyr Asn Met Gly Thr Ala Ile Leu Ala Met Ser 
            180                 185                 190         


Gly Pro Lys Tyr Val Ile Phe Lys Val Pro Ala Thr Thr Ser Asp Ile 
        195                 200                 205             


Lys Asp Asn Gly Lys Thr Asn Leu Ala Leu Lys Asn Leu Gln Gln Ile 
    210                 215                 220                 


Cys Ala Ile Pro Phe Arg Ser Lys Leu Tyr Pro Ser Tyr Tyr His Ser 
225                 230                 235                 240 


Phe Gly Met Thr Gln Asn Tyr Ile Ile Phe Val Glu Gln Pro Phe Lys 
                245                 250                 255     


Leu Asp Ile Ile Arg Leu Ala Thr Ala Tyr Phe Arg Arg Thr Thr Trp 
            260                 265                 270         


Gly Lys Cys Leu Phe Tyr Asp Gln Asp Asp Val Thr Leu Phe His Ile 
        275                 280                 285             


Ile Asn Arg Lys Thr Gly Asp Ala Val Asn Thr Lys Phe Tyr Gly Asp 
    290                 295                 300                 


Ala Leu Val Val Phe His His Ile Asn Ala Tyr Glu Glu Asp Gly His 
305                 310                 315                 320 


Ile Val Phe Asp Leu Ile Ser Tyr Lys Asp Ser Ser Leu Tyr Asp Leu 
                325                 330                 335     


Phe Tyr Ile Asp Tyr Met Lys Gln Glu Ala Pro Lys Phe Thr Glu Thr 
            340                 345                 350         


Ser Lys Ala Phe Ser Arg Pro Val Cys Gln Arg Phe Val Ile Pro Leu 
        355                 360                 365             


Asn Ala Asp Leu Lys Gly Asn Pro Leu Gly Lys Asn Leu Val Arg Leu 
    370                 375                 380                 


Glu Asp Thr Ser Ala Thr Ala Val Phe Gln Met Asp Gly Ser Leu Tyr 
385                 390                 395                 400 


Cys Thr Pro Glu Thr Leu Phe Gln Gly Leu Glu Leu Pro Ser Ile Asn 
                405                 410                 415     


Tyr Gln Tyr Asn Gly Lys Lys Tyr Arg Tyr Phe Tyr Gly Ser Met Met 
            420                 425                 430         


Asp Trp Ser Pro Gln Ala Asn Lys Ile Ala Lys Val Asp Val Asp Thr 
        435                 440                 445             


Lys Thr His Leu Glu Trp Thr Glu Glu Asp Cys Tyr Pro Ser Glu Pro 
    450                 455                 460                 


Lys Phe Val Ala Ser Pro Gly Ala Val Asp Glu Asp Asn Gly Val Ile 
465                 470                 475                 480 


Leu Ser Ser Val Val Ser Val Asn Pro Lys Lys Ser Pro Phe Met Leu 
                485                 490                 495     


Val Leu Asp Ala Lys Thr Leu Lys Glu Ile Ala Arg Ala Ser Ile Asp 
            500                 505                 510         


Ala Thr Val His Leu Asp Leu His Gly Ile Phe Ile Pro Gln Glu Thr 
        515                 520                 525             


Glu Leu Lys 
    530     


<210>  14
<211>  1596
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yarrowia codon-optimized IpBCO

<400>  14
atggaggcca ttttctgtcg aaacggcacc gagactcccg agcccgtcaa ggctgttgtg       60

tccggtgcta tccccccttg gcttcaggga acccttctcc gaaacggacc cggccttttc      120

tccattggta agacttccta caaccactgg tttgacggac tctctcttat tcactctttc      180

acctttaagc acggtgatgt ttactaccga tctaagttcc tccgatccga tacctacaag      240

aagaacattg ctgccaaccg aatcgttgtg tctgagtttg gcactatggt ctaccccgat      300

ccctgcaaga acattttctc taaggccttc acttacctgc tcaactctat tcccgatttc      360

accgacaaca accttgtctc tattattaag tacggcgatg actactacac ttcttccgag      420

attaactaca tcaaccagat caaccccgtt actctcgaca ctattggacg agccaactac      480

cgaaactaca tttcccttaa ccttgctact gcccaccctc actacgatga cgagggaaac      540

acctacaaca tgggcactgc tatcctggct atgtctggac ccaagtacgt catcttcaag      600

gtgcccgcta ctacctctga tattaaggac aacggaaaga ctaaccttgc tctgaagaac      660

ctgcagcaga tctgcgccat tcctttccga tctaagctct acccttctta ctaccactcc      720

tttggtatga ctcagaacta catcattttc gttgagcagc ccttcaagct ggacattatt      780

cgactggcca ctgcttactt ccgacgaacc acctggggca agtgcctctt ttacgaccag      840

gacgatgtta ctctcttcca cattatcaac cgaaagactg gtgacgccgt gaacactaag      900

ttctacggtg atgctctcgt ggttttccac cacatcaacg cctacgagga ggacggccac      960

atcgtttttg acctgatctc ttacaaggac tcttctctct acgacctttt ctacattgac     1020

tacatgaagc aggaggctcc taagttcact gagacttcca aggctttttc tcgacccgtc     1080

tgtcagcgat tcgtcatccc tctcaacgct gacctcaagg gaaaccccct gggcaagaac     1140

cttgtccgac ttgaggacac ttctgctacc gctgtgttcc agatggacgg ttccctgtac     1200

tgtactcccg agactctctt tcagggtctt gagctccctt ccattaacta ccagtacaac     1260

ggaaagaagt accgatactt ctacggctct atgatggatt ggtcccctca ggctaacaag     1320

atcgctaagg tggacgttga taccaagact caccttgagt ggaccgagga ggattgctac     1380

ccttctgagc ctaagtttgt cgcttcccct ggcgctgtcg atgaggataa cggtgtgatc     1440

ctgtcttctg ttgtctccgt caaccccaag aagtccccct ttatgctcgt gctcgatgct     1500

aagaccctca aggagatcgc tcgagcctct attgacgcca ctgttcacct cgacctccac     1560

ggaattttca tccctcagga gactgagctt aagtaa                               1596


<210>  15
<211>  527
<212>  PRT
<213>  Esox lucius

<400>  15

Met Ala Gln Ile Ile Phe Gly Lys Asn Gly Thr Glu Ser Pro Glu Pro 
1               5                   10                  15      


Val Lys Ala Glu Ile Thr Gly Cys Ile Pro Glu Trp Leu Gln Gly Thr 
            20                  25                  30          


Leu Leu Arg Asn Gly Pro Gly Leu Phe Lys Val Gly Asp Thr Glu Tyr 
        35                  40                  45              


Asn His Trp Phe Asp Gly Met Ala Leu Ile His Ser Phe Thr Phe Lys 
    50                  55                  60                  


Asp Gly Asp Val Tyr Tyr Arg Ser Lys Phe Leu Arg Ser Asp Thr Phe 
65                  70                  75                  80  


Gln Lys Asn Thr Lys Ala Asn Lys Ile Val Val Ser Glu Phe Gly Thr 
                85                  90                  95      


Met Ile Tyr Pro Asp Pro Cys Lys Asn Met Phe Ser Lys Ala Phe Ser 
            100                 105                 110         


Tyr Leu Leu Ala Ala Ile Pro Asp Phe Thr Asp Asn Asn Leu Ile Asn 
        115                 120                 125             


Ile Ile Arg Tyr Gly Glu Asp Tyr Tyr Ala Ser Ser Glu Ile Asn Tyr 
    130                 135                 140                 


Ile Asn Gln Ile Asp Pro Val Thr Leu Glu Val Ile Gly Lys Met Asn 
145                 150                 155                 160 


Tyr Arg Lys His Ile Ser Leu Asn Leu Ala Thr Ala His Pro His Tyr 
                165                 170                 175     


Asp Glu Glu Gly Asn Thr Tyr Asn Met Gly Ile Ala Leu Met Arg Phe 
            180                 185                 190         


Gly Met Pro Lys Tyr Val Ile Phe Lys Val Pro Val Asp Ala Ser Asp 
        195                 200                 205             


Lys Glu Gly Lys Lys Pro Ala Leu Glu Glu Val Glu Gln Val Cys Asn 
    210                 215                 220                 


Ile Pro Phe Arg Ser Thr Leu Phe Pro Ser Tyr Phe His Ser Phe Gly 
225                 230                 235                 240 


Met Ser Glu Asn Tyr Ile Ile Phe Val Glu Gln Pro Phe Lys Leu Asp 
                245                 250                 255     


Ile Leu Arg Leu Ala Thr Ala Asn Phe Arg Gly Ser Thr Trp Gly Ser 
            260                 265                 270         


Cys Leu Lys Tyr Asp Lys Glu Asp Ile Thr Leu Ile His Leu Val Asp 
        275                 280                 285             


Lys Lys Thr Gly Lys Ala Val Ser Thr Lys Phe Tyr Ala Asp Ala Leu 
    290                 295                 300                 


Val Val Phe His His Ile Asn Ala Tyr Glu Asp Asp Asn His Val Val 
305                 310                 315                 320 


Phe Asp Met Ile Thr Tyr Lys Asp Ser Asn Leu Tyr Glu Met Phe Tyr 
                325                 330                 335     


Leu Ala Asn Met Arg Glu Glu Ser Asn Lys Phe Ile Glu Asp Lys Val 
            340                 345                 350         


Asn Phe Ser Gln Pro Ile Cys Gln Arg Phe Val Leu Pro Leu Asn Val 
        355                 360                 365             


Asp Lys Asp Thr Thr Lys Gly Thr Asn Met Val Met Leu Lys Asn Thr 
    370                 375                 380                 


Thr Ala Lys Ala Val Met Gln Asp Asp Gly Ser Val Tyr Cys Lys Pro 
385                 390                 395                 400 


Asp Thr Ile Phe Ala Gly Leu Glu Leu Pro Gly Ile Asn Tyr Lys Phe 
                405                 410                 415     


Asn Gly Lys Lys Tyr Arg Tyr Phe Tyr Gly Ser Arg Val Glu Trp Thr 
            420                 425                 430         


Pro Phe Pro Asn Lys Ile Gly Lys Val Asp Ile Leu Thr Lys Lys His 
        435                 440                 445             


Ile Glu Trp Thr Glu Glu Glu Cys Tyr Pro Ser Glu Pro Val Phe Val 
    450                 455                 460                 


Ala Ser Pro Gly Ala Met Glu Glu Asp Asp Gly Val Ile Leu Ser Ser 
465                 470                 475                 480 


Ile Val Ser Leu Asn Pro Asn Lys Ser Pro Phe Met Leu Val Leu Asn 
                485                 490                 495     


Ala Lys Asn Phe Glu Glu Ile Ala Arg Ala Ser Ile Asp Ala Ser Val 
            500                 505                 510         


His Leu Asp Leu His Gly Leu Phe Ile Pro Ser Gln Lys Thr Asn 
        515                 520                 525         


<210>  16
<211>  1584
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yarrowia codon-optimized ElBCO

<400>  16
atggctcaga ttatttttgg caagaacggc actgagtctc ctgagcctgt caaggccgag       60

attaccggat gtatccctga gtggctccag ggtactctcc ttcgaaacgg tcccggtctt      120

ttcaaggtgg gtgataccga gtacaaccac tggttcgatg gcatggccct gattcactct      180

tttaccttca aggatggtga cgtgtactac cgatctaagt tccttcgatc cgacaccttc      240

cagaagaaca ctaaggctaa caagattgtt gtgtctgagt ttggcaccat gatttaccct      300

gacccctgca agaacatgtt ttccaaggct ttctcctacc tccttgctgc catccctgac      360

ttcaccgata acaacctgat taacattatc cgatacggtg aggactacta cgcctcttcc      420

gagatcaact acatcaacca gattgaccct gttaccctgg aggtgattgg aaagatgaac      480

taccgaaagc acatttctct gaaccttgct actgcccacc ctcactacga cgaggaggga      540

aacacttaca acatgggaat cgccctcatg cgatttggca tgcccaagta cgtcatcttc      600

aaggttcctg tcgatgcttc tgataaggag ggcaagaagc ctgcccttga ggaggtggag      660

caggtctgca acattccctt tcgatctacc ctcttcccct cttacttcca ctcttttggc      720

atgtctgaga actacatcat ctttgtcgag cagcctttca agctggacat cctccgactg      780

gccactgcta acttccgagg atctacctgg ggttcctgcc tgaagtacga caaggaggac      840

attactctca tccacctggt cgacaagaag actggtaagg ctgtttccac caagttctac      900

gctgatgctc tggttgtttt ccaccacatt aacgcctacg aggacgacaa ccacgtggtt      960

ttcgatatga tcacctacaa ggactccaac ctgtacgaga tgttctacct tgctaacatg     1020

cgagaggagt ctaacaagtt cattgaggac aaggtcaact tctcccagcc tatctgccag     1080

cgatttgtcc tccccctcaa cgttgacaag gataccacta agggaaccaa catggtgatg     1140

ctcaagaaca ctaccgccaa ggccgtgatg caggatgacg gctctgtgta ctgcaagcct     1200

gacaccattt ttgctggtct tgagctccct ggcattaact acaagttcaa cggcaagaag     1260

taccgatact tttacggctc tcgagtggag tggactccct tccctaacaa gattggaaag     1320

gtggacattc tgaccaagaa gcacattgag tggaccgagg aggagtgtta cccctctgag     1380

cccgtttttg ttgcctcccc cggagctatg gaggaggatg acggagtcat tctttcttct     1440

attgtctctc tcaaccctaa caagtccccc ttcatgcttg tcctcaacgc taagaacttt     1500

gaggagattg ctcgagcctc catcgatgcc tctgttcacc tcgatctcca cggactcttc     1560

attccctctc agaagactaa ctag                                            1584


<210>  17
<211>  531
<212>  PRT
<213>  Latimeria chalumnae

<400>  17

Met Gln Ser Leu Phe Gly Lys Asn Lys Arg Glu Cys Pro Glu Pro Ile 
1               5                   10                  15      


Lys Ala Glu Val Lys Gly Gln Ile Pro Ala Trp Leu Gln Gly Thr Leu 
            20                  25                  30          


Leu Arg Asn Gly Pro Gly Met His Thr Val Gly Glu Thr Ser Tyr Asn 
        35                  40                  45              


His Trp Phe Asp Gly Leu Ala Leu Met His Ser Phe Thr Phe Lys Asp 
    50                  55                  60                  


Gly Glu Val Phe Tyr Gln Ser Lys Tyr Leu Arg Ser Asp Thr Tyr Lys 
65                  70                  75                  80  


Lys Asn Met Glu Ala Asn Arg Ile Val Val Ser Glu Phe Gly Thr Met 
                85                  90                  95      


Ala Tyr Pro Asp Pro Cys Lys Asn Ile Phe Ser Lys Ala Phe Ser Tyr 
            100                 105                 110         


Leu Ser His Thr Ile Pro Glu Phe Thr Asp Asn Cys Leu Ile Asn Ile 
        115                 120                 125             


Met Lys Cys Gly Glu Asp Tyr Tyr Ala Val Thr Glu Thr Asn Phe Ile 
    130                 135                 140                 


Arg Lys Ile Asp Pro Lys Ser Leu Asp Thr Leu Glu Lys Val Asp Tyr 
145                 150                 155                 160 


Thr Lys Tyr Ile Ala Leu Asn Leu Ala Ser Ser His Pro His Tyr Asp 
                165                 170                 175     


Ala Ala Gly Asp Thr Ile Asn Met Gly Thr Ser Ile Ala Asp Lys Gly 
            180                 185                 190         


Lys Thr Lys Tyr Leu Ile Val Lys Ile Pro Asn Met Lys Pro Val Glu 
        195                 200                 205             


Ser Glu Lys Lys Lys Lys Val Tyr Phe Lys Asn Leu Glu Val Leu Cys 
    210                 215                 220                 


Ser Ile Pro Ser His Gly Arg Leu Asn Pro Ser Tyr Tyr His Ser Phe 
225                 230                 235                 240 


Gly Ile Thr Glu Asn Tyr Ile Val Phe Val Glu Gln Pro Phe Lys Leu 
                245                 250                 255     


Asp Leu Leu Lys Leu Ala Thr Ala Tyr Phe Arg Gly Ile Asn Trp Ala 
            260                 265                 270         


Ser Cys Leu Asn Phe His Ser Glu Asp Lys Thr Phe Ile His Ile Ile 
        275                 280                 285             


Asp Arg Arg Thr Lys Thr Ser Val Ser Thr Lys Phe His Thr Asp Ala 
    290                 295                 300                 


Leu Val Leu Tyr His His Val Asn Ala Tyr Glu Glu Asp Gly His Val 
305                 310                 315                 320 


Val Phe Asp Val Ile Ala Tyr Asn Asp Ser Ser Leu Tyr Asp Met Phe 
                325                 330                 335     


Tyr Leu Ala Asn Val Arg Gln Glu Ser Ala Glu Phe Glu Ala Lys Asn 
            340                 345                 350         


Thr Ser Ser Ser Lys Pro Ala Cys Arg Arg Phe Val Ile Pro Leu Gln 
        355                 360                 365             


Pro Asp Lys Asp Ala Glu Leu Gly Thr Asn Leu Val Lys Leu Ala Ser 
    370                 375                 380                 


Thr Thr Ala Asp Ala Ile Lys Glu Lys Asp Ser Ile Tyr Cys His Pro 
385                 390                 395                 400 


Glu Ile Leu Val Glu Asp Ile Glu Leu Pro Arg Ile Asn Tyr Asn Tyr 
                405                 410                 415     


Asn Gly Lys Lys Tyr Arg Tyr Ile Tyr Val Thr Gly Ile Ala Trp Lys 
            420                 425                 430         


Pro Ile Pro Thr Lys Ile Val Lys Phe Asp Thr Leu Thr Arg Lys Ser 
        435                 440                 445             


Val Glu Trp Gln Glu Glu Asp Cys Trp Pro Ala Glu Pro Val Phe Val 
    450                 455                 460                 


Pro Ser Pro Asp Ala Lys Glu Glu Asp Asp Gly Ile Val Leu Ser Ser 
465                 470                 475                 480 


Ile Val Cys Thr Ser Pro Asn Lys Phe Pro Phe Leu Leu Ile Leu Asp 
                485                 490                 495     


Ala Lys Thr Phe Thr Glu Leu Ala Arg Ala Ser Ile Asn Ala Asp Val 
            500                 505                 510         


His Leu Asp Leu His Gly Tyr Phe Ile Pro Glu Lys Lys Lys Ala Gln 
        515                 520                 525             


Ile Thr His 
    530     


<210>  18
<211>  1596
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Yarrowia codon-optimized LcBCO

<400>  18
atgcagtctc tgttcggtaa gaacaagcga gagtgtcctg agcccattaa ggctgaggtg       60

aagggtcaga ttcctgcttg gctccagggt actctccttc gaaacggccc tggcatgcac      120

accgttggcg agacttctta caaccactgg ttcgacggac tcgctcttat gcactccttc      180

acctttaagg atggtgaggt tttttaccag tctaagtacc tgcgatccga cacctacaag      240

aagaacatgg aggccaaccg aattgtcgtg tctgagttcg gaaccatggc ctaccccgat      300

ccctgcaaga acattttttc caaggctttt tcttaccttt ctcacaccat ccctgagttt      360

accgacaact gtctgatcaa cattatgaag tgtggtgagg attactacgc tgttactgag      420

actaacttca tccgaaagat tgatcccaag tccctcgaca ccctggagaa ggttgactac      480

accaagtaca ttgctcttaa cctggcttcc tcccaccccc actacgatgc tgctggtgat      540

accattaaca tgggcacctc tatcgctgat aagggaaaga ctaagtacct gattgttaag      600

attcccaaca tgaagcccgt tgagtctgag aagaagaaga aggtctactt taagaacctg      660

gaggtgctct gctccatccc ttctcacgga cgacttaacc cttcttacta ccactccttt      720

ggcatcactg agaactacat cgttttcgtg gagcagccct ttaagctgga ccttctcaag      780

ctggccaccg cctacttccg aggtattaac tgggcctctt gtcttaactt ccactccgag      840

gacaagactt tcattcacat catcgatcga cgaaccaaga cctccgtttc cactaagttt      900

cacaccgatg ctctcgttct ttaccaccac gtcaacgctt acgaggagga tggccacgtt      960

gttttcgatg tcattgccta caacgactct tctctctacg atatgtttta cctcgccaac     1020

gttcgacagg agtctgccga gtttgaggct aagaacacct cttcctccaa gcctgcttgt     1080

cgacgatttg tcattcccct gcagcctgac aaggatgctg agctgggcac taacctggtc     1140

aagctcgctt ccactaccgc cgacgccatt aaggagaagg actccattta ctgccaccct     1200

gagatcctgg ttgaggatat tgagctccct cgaattaact acaactacaa cggcaagaag     1260

taccgataca tttacgttac tggtatcgcc tggaagccca ttcccactaa gattgtcaag     1320

tttgacactc tcactcgaaa gtccgtggag tggcaggagg aggactgttg gcccgccgag     1380

cctgtctttg ttccttcccc cgatgccaag gaggaggacg atggtattgt tctttcttcc     1440

atcgtgtgta cttcccctaa caagtttccc ttcctcctta ttctggacgc caagaccttt     1500

accgagctcg ctcgagcttc tattaacgcc gatgtccacc tcgaccttca cggatacttt     1560

atccctgaga agaagaaggc ccagatcacc cactag                               1596


