                               SEQUENCE LISTING

<110> ZBIOTICS COMPANY
 
<120> GENE EXPRESSION SYSTEM FOR PROBIOTIC MICROORGANISMS

<130> 1642-001-US

<140>
<141>

<150> 62/558,346
<151> 2017-09-13

<160> 22    

<170> PatentIn version 3.5

<210> 1
<211> 23
<212> DNA
<213> Bacillus subtilis 

<400> 1
taagggcaca aggacgtgcc tta                                               23


<210> 2
<211> 12
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 2
gcacaagaac gt                                                           12


<210> 3
<211> 13
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
atttagggag gaa                                                          13


<210> 4
<211> 506
<212> PRT
<213> Cupriavidus necator

<400> 4
Met Asn Met Ala Glu Ile Ala Gln Leu Gly Val Ser Asn Pro Tyr Lys 
1               5                   10                  15      


Gln Gln Tyr Glu Asn Tyr Ile Gly Gly Ala Trp Val Pro Pro Ala Gly 
            20                  25                  30          


Gly Glu Tyr Phe Glu Ser Thr Thr Pro Ile Thr Gly Lys Pro Phe Thr 
        35                  40                  45              


Arg Val Pro Arg Ser Gly Gln Gln Asp Val Asp Ala Ala Leu Asp Ala 
    50                  55                  60                  


Ala His Ala Ala Lys Ala Ala Trp Ala Arg Thr Ser Thr Thr Glu Arg 
65                  70                  75                  80  


Ala Asn Ile Leu Asn Arg Ile Ala Asp Arg Ile Glu Ala Asn Leu Lys 
                85                  90                  95      


Leu Leu Ala Val Ala Glu Ser Ile Asp Asn Gly Lys Pro Val Arg Glu 
            100                 105                 110         


Thr Thr Ala Ala Asp Leu Pro Leu Ala Val Asp His Phe Arg Tyr Phe 
        115                 120                 125             


Ala Gly Cys Ile Arg Ala Gln Glu Gly Gly Ile Ser Glu Ile Asp Ala 
    130                 135                 140                 


Asp Thr Ile Ala Tyr His Phe His Glu Pro Leu Gly Val Val Gly Gln 
145                 150                 155                 160 


Ile Ile Pro Trp Asn Phe Pro Leu Leu Met Ala Thr Trp Lys Leu Ala 
                165                 170                 175     


Pro Ala Leu Ala Ala Gly Asn Cys Val Val Leu Lys Pro Ala Glu Gln 
            180                 185                 190         


Thr Pro Ala Ser Ile Leu Val Leu Met Glu Val Ile Gly Asp Leu Leu 
        195                 200                 205             


Pro Pro Gly Val Val Asn Val Ile Asn Gly Phe Gly Leu Glu Ala Gly 
    210                 215                 220                 


Lys Pro Leu Ala Ser Ser Pro Arg Ile Ser Lys Val Ala Phe Thr Gly 
225                 230                 235                 240 


Glu Thr Thr Thr Gly Arg Leu Ile Met Gln Tyr Ala Ser Gln Asn Leu 
                245                 250                 255     


Ile Pro Val Thr Leu Glu Leu Gly Gly Lys Ser Pro Asn Ile Phe Phe 
            260                 265                 270         


Glu Asp Val Leu Ala Ala Asp Asp Ala Phe Phe Asp Lys Ala Leu Glu 
        275                 280                 285             


Gly Phe Ala Met Phe Ala Leu Asn Gln Gly Glu Val Cys Thr Cys Pro 
    290                 295                 300                 


Ser Arg Ala Leu Ile Gln Glu Ser Ile Tyr Asp Arg Phe Met Glu Arg 
305                 310                 315                 320 


Ala Leu Lys Arg Val Ala Ala Ile Arg Gln Gly His Pro Leu Asp Thr 
                325                 330                 335     


Gly Thr Met Ile Gly Ala Gln Ala Ser Ala Glu Gln Leu Glu Lys Ile 
            340                 345                 350         


Leu Ser Tyr Ile Asp Leu Gly Arg Lys Glu Gly Ala Gln Cys Leu Thr 
        355                 360                 365             


Gly Gly Glu Arg Asn Val Leu Asp Gly Asp Leu Ala Gly Gly Tyr Tyr 
    370                 375                 380                 


Val Lys Pro Thr Val Phe Ala Gly His Asn Lys Met Arg Ile Phe Gln 
385                 390                 395                 400 


Glu Glu Ile Phe Gly Pro Val Val Ser Val Thr Thr Phe Lys Asp Glu 
                405                 410                 415     


Glu Glu Ala Leu Ala Ile Ala Asn Asp Thr Leu Tyr Gly Leu Gly Ala 
            420                 425                 430         


Gly Val Trp Thr Arg Asp Gly Ala Arg Ala Phe Arg Met Gly Arg Gly 
        435                 440                 445             


Ile Gln Ala Gly Arg Val Trp Thr Asn Cys Tyr His Ala Tyr Pro Ala 
    450                 455                 460                 


His Ala Ala Phe Gly Gly Tyr Lys Gln Ser Gly Ile Gly Arg Glu Asn 
465                 470                 475                 480 


His Arg Met Met Leu Asp His Tyr Gln Gln Thr Lys Asn Leu Leu Val 
                485                 490                 495     


Ser Tyr Ser Pro Asn Ala Leu Gly Phe Phe 
            500                 505     


<210> 5
<211> 517
<212> PRT
<213> Homo sapiens

<400> 5
Met Leu Arg Ala Ala Ala Arg Phe Gly Pro Arg Leu Gly Arg Arg Leu 
1               5                   10                  15      


Leu Ser Ala Ala Ala Thr Gln Ala Val Pro Ala Pro Asn Gln Gln Pro 
            20                  25                  30          


Glu Val Phe Cys Asn Gln Ile Phe Ile Asn Asn Glu Trp His Asp Ala 
        35                  40                  45              


Val Ser Arg Lys Thr Phe Pro Thr Val Asn Pro Ser Thr Gly Glu Val 
    50                  55                  60                  


Ile Cys Gln Val Ala Glu Gly Asp Lys Glu Asp Val Asp Lys Ala Val 
65                  70                  75                  80  


Lys Ala Ala Arg Ala Ala Phe Gln Leu Gly Ser Pro Trp Arg Arg Met 
                85                  90                  95      


Asp Ala Ser His Arg Gly Arg Leu Leu Asn Arg Leu Ala Asp Leu Ile 
            100                 105                 110         


Glu Arg Asp Arg Thr Tyr Leu Ala Ala Leu Glu Thr Leu Asp Asn Gly 
        115                 120                 125             


Lys Pro Tyr Val Ile Ser Tyr Leu Val Asp Leu Asp Met Val Leu Lys 
    130                 135                 140                 


Cys Leu Arg Tyr Tyr Ala Gly Trp Ala Asp Lys Tyr His Gly Lys Thr 
145                 150                 155                 160 


Ile Pro Ile Asp Gly Asp Phe Phe Ser Tyr Thr Arg His Glu Pro Val 
                165                 170                 175     


Gly Val Cys Gly Gln Ile Ile Pro Trp Asn Phe Pro Leu Leu Met Gln 
            180                 185                 190         


Ala Trp Lys Leu Gly Pro Ala Leu Ala Thr Gly Asn Val Val Val Met 
        195                 200                 205             


Lys Val Ala Glu Gln Thr Pro Leu Thr Ala Leu Tyr Val Ala Asn Leu 
    210                 215                 220                 


Ile Lys Glu Ala Gly Phe Pro Pro Gly Val Val Asn Ile Val Pro Gly 
225                 230                 235                 240 


Phe Gly Pro Thr Ala Gly Ala Ala Ile Ala Ser His Glu Asp Val Asp 
                245                 250                 255     


Lys Val Ala Phe Thr Gly Ser Thr Glu Ile Gly Arg Val Ile Gln Val 
            260                 265                 270         


Ala Ala Gly Ser Ser Asn Leu Lys Arg Val Thr Leu Glu Leu Gly Gly 
        275                 280                 285             


Lys Ser Pro Asn Ile Ile Met Ser Asp Ala Asp Met Asp Trp Ala Val 
    290                 295                 300                 


Glu Gln Ala His Phe Ala Leu Phe Phe Asn Gln Gly Gln Cys Cys Cys 
305                 310                 315                 320 


Ala Gly Ser Arg Thr Phe Val Gln Glu Asp Ile Tyr Asp Glu Phe Val 
                325                 330                 335     


Glu Arg Ser Val Ala Arg Ala Lys Ser Arg Val Val Gly Asn Pro Phe 
            340                 345                 350         


Asp Ser Lys Thr Glu Gln Gly Pro Gln Val Asp Glu Thr Gln Phe Lys 
        355                 360                 365             


Lys Ile Leu Gly Tyr Ile Asn Thr Gly Lys Gln Glu Gly Ala Lys Leu 
    370                 375                 380                 


Leu Cys Gly Gly Gly Ile Ala Ala Asp Arg Gly Tyr Phe Ile Gln Pro 
385                 390                 395                 400 


Thr Val Phe Gly Asp Val Gln Asp Gly Met Thr Ile Ala Lys Glu Glu 
                405                 410                 415     


Ile Phe Gly Pro Val Met Gln Ile Leu Lys Phe Lys Thr Ile Glu Glu 
            420                 425                 430         


Val Val Gly Arg Ala Asn Asn Ser Thr Tyr Gly Leu Ala Ala Ala Val 
        435                 440                 445             


Phe Thr Lys Asp Leu Asp Lys Ala Asn Tyr Leu Ser Gln Ala Leu Gln 
    450                 455                 460                 


Ala Gly Thr Val Trp Val Asn Cys Tyr Asp Val Phe Gly Ala Gln Ser 
465                 470                 475                 480 


Pro Phe Gly Gly Tyr Lys Met Ser Gly Ser Gly Arg Glu Leu Gly Glu 
                485                 490                 495     


Tyr Gly Leu Gln Ala Tyr Thr Glu Val Lys Thr Val Thr Val Lys Val 
            500                 505                 510         


Pro Gln Lys Asn Ser 
        515         


<210> 6
<211> 915
<212> DNA
<213> Bacillus subtilis

<400> 6
atgagaatta accacaatat tgcagcgctt aacacactga accgtttgtc ttcaaacaac       60

agtgcgagcc aaaagaacat ggagaaactt tcttcaggtc ttcgcatcaa ccgtgcggga      120

gatgacgcag caggtcttgc gatctctgaa aaaatgagag gacaaatcag aggtcttgaa      180

atggcttcta aaaactctca agacggaatc tctcttatcc aaacagctga gggtgcatta      240

actgaaactc atgcgatcct tcaacgtgtt cgtgagctag ttgttcaagc tggaaacact      300

ggaactcagg acaaagcaac tgatttgcaa tctattcaag atgaaatttc agctttaaca      360

gatgaaatcg atggtatttc aaatcgtaca gaattcaatg gtaagaaatt gctcgatggc      420

acttacaaag ttgacacagc tactcctgca aatcaaaaga acttggtatt ccaaatcgga      480

gcaaatgcta cacagcaaat ctctgtaaat attgaggata tgggtgctga cgctcttgga      540

attaaagaag ctgatggttc aattgcagct cttcattcag ttaatgatct tgacgtaaca      600

aaattcgcag ataatgcagc agatactgct gatatcggtt tcgatgctca attgaaagtt      660

gttgatgaag cgatcaacca agtttcttct caacgtgcta agcttggtgc ggtacaaaat      720

cgtctagagc acacaattaa caacttaagc gcttctggtg aaaacttgac agctgctgag      780

tctcgtatcc gtgacgttga catggctaaa gagatgagcg aattcacaaa gaacaacatt      840

ctttctcagg cttctcaagc tatgcttgct caagcaaacc aacagccgca aaacgtactt      900

caattattac gttaa                                                       915


<210> 7
<211> 276
<212> DNA
<213> Bacillus subtilis

<400> 7
ggaattgacg ccccaaagca tattgatatt cacaggaaag aaatttactt gaccattcag       60

gaagaaaata accgtgcagc agcgttatcc agcgatgtga tctccgcatt atcctcacaa      120

aaaaagtgag gattttttta tttttgtatt aacaaaatca gagacaatcc gatattaatg      180

atgtagccgg gaggaggcgc aaaagactca gccagttaca aaataagggc acaaggacgt      240

gccttaacaa catattcagg gaggaacaaa acaatg                                276


<210> 8
<211> 12
<212> DNA
<213> Bacillus subtilis

<400> 8
gcacaaggac gt                                                           12


<210> 9
<211> 13
<212> DNA
<213> Bacillus subtilis

<400> 9
attcagggag gaa                                                          13


<210> 10
<211> 225
<212> DNA
<213> Bacillus subtilis

<400> 10
atgctagttt tatcgcggaa aataaacgaa gcgattcaaa taggtgctga tattgaagta       60

aaagtgattg cggttgaagg ggatcaagtg aagcttggaa ttgacgcccc aaagcatatt      120

gatattcaca ggaaagaaat ttacttgacc attcaggaag aaaataaccg tgcagcagcg      180

ttatccagcg atgtgatctc cgcattatcc tcacaaaaaa agtga                      225


<210> 11
<211> 267
<212> DNA
<213> Bacillus subtilis

<400> 11
atgaaaatca atcaatttgg aacacaatcc gttaatccat atcaaaaaaa ttatgataag       60

caagcggtgc aaaaaactgt tgcacaacct caagataaaa ttgaaatttc atcacaggct      120

aaagaaatgc aacatgcatc cgacgcagtc actggttcac gacaggaaaa aattgcgcag      180

cttaaagcgc aaattgaaaa cgggtcatac aaagtagacg caaatcatat tgcgaaaaat      240

atgattaatt tttataaaaa gcaataa                                          267


<210> 12
<211> 765
<212> DNA
<213> Bacillus subtilis

<400> 12
atgcaatcct tgaattatga agatcaggtg ctttggacgc gctggaaaga gtggaaagat       60

cctaaagccg gtgacgactt aatgcgccgt tacatgccgc ttgtcacata tcatgtaggc      120

agaatttctg tcggactgcc gaaatcagtg cataaagacg atcttatgag ccttggtatg      180

cttggtttat atgatgccct tgaaaaattt gaccccagcc gggacttaaa atttgatacc      240

tacgcctcgt ttagaattcg cggcgcaatc atagacgggc ttcgtaaaga agattggctg      300

cccagaacct cgcgcgaaaa aacaaaaaag gttgaagcag caattgaaaa gcttgaacag      360

cggtatcttc ggaatgtatc gcccgcggaa attgcagagg aactcggaat gacggtacag      420

gatgtcgtgt caacaatgaa tgaaggtttt tttgcaaatc tgctgtcaat tgatgaaaag      480

ctccatgatc aagatgacgg ggaaaacatt caagtcatga tcagagatga caaaaatgtt      540

ccgcctgaag aaaagattat gaaggatgaa ctgattgcac agcttgcgga aaaaattcac      600

gaactctctg aaaaagaaca gctggttgtc agtttgttct acaaagagga gttgacactg      660

acagaaatcg gacaagtatt aaatctttct acgtcccgca tatctcagat ccattcaaag      720

gcattattta aattaaagaa tctgctggaa aaagtgatac aataa                      765


<210> 13
<211> 21
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 13
ctggcgttac ccaacttaat c                                                 21


<210> 14
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 14
cttggcgtaa tcatggtcat ag                                                22


<210> 15
<211> 22
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 15
gaggaaacag gtgtggaaga ag                                                22


<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      primer

<400> 16
ggtcatcttc tgtctgcgtg                                                   20


<210> 17
<211> 1650
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 17
ctatgaccat gattacgcca agtgaataat gagaaacagt caaagaaaaa gaaaacagaa       60

cgcctgctgt cagagtgcat ttttgataca aaaaataatt cagcagaagg tatgaatatc      120

attttaatag acgatcttta tacaacaggc gccaccttgc acttcgcagc ccgctgctta      180

ttagaaaaag gaaaagccgc ttcagtgtca tcttttacct tgatcagaag ctaaatgatt      240

ctgtttttat gccgatataa tcactagaaa ttgacacagg catattatct aataaggaga      300

aaaaaagatg ggagaactgg ctaattgtcc gaaatgcaat gctttatttt taaaaacaaa      360

gctgcaaacc gtatgtcagg cgtgtattaa ggaagaagaa aaatcatttg agactgtcta      420

taaattttta agaaaacagg aaaaccggca atcaactttg agccggataa ctgaggaaac      480

aggtgtggaa gaagagctga tattgaaatt catcaggcag aagcgaattc agatcactca      540

tcttcctaat ttggcatacc cttgtgaaag gtgcgggaca tcgattagag aaggcaagtt      600

ctgcaaggct tgccagtctg atattaagga tcaaatggat catttgaacc acgaggatgc      660

tctgaaaatc gagaaagaaa atagtaaaaa agacacatac tatgcctata ataccaaaaa      720

cagctgattc cctaaactaa ctgaaaacgc agtcgataaa agggttaaga ttgtttaaag      780

actgcaacgg aaagcgagag gaatcctatg aaaatcaatc aactgcagtt ttataaaaag      840

caataaaaaa ggagaaagcc catgtcagcg aaggcaatta ttgaacaatt gaagcgactt      900

tgcgttctgc atgagcacct gctcacgctg tctgaagaaa agacggaagc gctcaaagcc      960

ggcaaaacaa aagagctttc taacattttg acaaaagagc aaaaatatat tcaagcaatc     1020

acgcagacag aagatgaccg gatcaaaaca acttcggcct ttctcggata tagcgaaaat     1080

aatactattt ccgcatgtat cgccaaaacc tcaggcagtg aaaaggaaga gctggaacaa     1140

ctatacgaat ctctttctca agttctcgga cgtctgaaaa aagtaaatga gatgaatagg     1200

cagctgacaa gagacgcgct gcaattcatc tctatttcgt acgatatgct ggttcctaag     1260

gaaaataact tcaattacag caaatcaatt aaagctgagc tgccgaaaag tagcaaaatg     1320

aaactgtttg attcaaaagc ttagcagaaa ggaattcaga aaatgacatc tacctttatg     1380

gggcttgaaa ctgcaaggcg ggcgttaagc gctcagcagg cagcgttaag cactactgca     1440

aataacgtgg caaatgccaa tactgatggt tatacaagac agcgggtctc attggaggca     1500

actgactatt tccctgctgt atctaaaaat gcagaaaaaa cagcgggaca aatgggtacg     1560

ggcgttcaag gaaaatcagt tgagagaata agagatatct ttcttgacta ccaataccgt     1620

cttcaaaacc tggcgttacc caacttaatc                                      1650


<210> 18
<211> 2360
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 18
ctatgaccat gattacgcca agtgaacaat gatcattcat acgaagtacc atggccaaat       60

gaacataaaa gaagaacaaa tcattctttt tgaaagcggg attccaggct ttttagaaga      120

aaaacagttc gtcatacttc cgctttcaga agactctcca ttcgtggcac tgcagtccgt      180

cacttcagaa aatcttgcgt ttatcgtcgt aagtccgttt atctttttta agaattatga      240

atttgatctt gatgaatcaa ctgctgaact tttggatatc gataatattc aagacgtaga      300

agtcatgaca atattgacta tggcagagcc atttgaaaag tctactgcga atttattggc      360

tcccattatt gtgaatcgca agaacatgat ggctaagcaa gtcgttttac acgactcctc      420

atatacgaca aagcatccga ttggaggaga atcatgctag ttttatcgcg gaaaataaac      480

gaagcgattc aaataggtgc tgatattgaa gtaaaagtga ttgcggttga aggggatcaa      540

gtgaagcttg gaattgacgc cccaaagcat attgatattc acaggaaaga aatttacttg      600

accattcagg aagaaaataa ccgtgcagca gcgttatcca gcgatgtgat ctccgcatta      660

tcctcacaaa aaaagtgagg atttttttat ttttgtatta acaaaatcag agacaatccg      720

atattaatga tgtagccggg aggaggcgca aaagactcag ccagttacaa aataagggca      780

caagaacgtg ccttaacaac atattcaggg aggaacaaaa caatgcgtaa aggagaagaa      840

cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa      900

ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt      960

atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac tttcggttat     1020

ggtgttcaat gctttgcgag atacccagat catatgaaac ggcatgactt tttcaagagt     1080

gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga cgggaactac     1140

aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat cgagttaaaa     1200

ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata caactataac     1260

tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa     1320

attagacaca acattgaaga tggaagcgtt caactagcag accattatca acaaaatact     1380

ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac acaatctgcc     1440

ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt tgtaacagct     1500

gctgggatta cacatggcat ggatgaacta tacaaataat tttaaaaaag accttggcgt     1560

tgccagggtc ttttaattta aatttctatc tcctaatcat tcctcatcct gtcactaact     1620

catgatataa taaccggatt ctccactaac tttttataaa tgtatttcca tacaagaaat     1680

ctaaaacaga agattttttt ccaaaaatat gtgtaatctt atctcgactt agtcgatata     1740

aacgatagat tggggcatag gggatgatca attgaacatt gaaaggctca ctacgttaca     1800

acctgtttgg gatcgttatg atactcaaat acataatcag aaagataatg ataacgaggt     1860

tcctgttcat caagtttcat ataccaatct tgctgaaatg gtgggggaaa tgaacaagct     1920

tttggaacct tcgcaagttc atctgaagtt cgagcttcat gacaagttaa atgaatacta     1980

tgtaaaggta atagaggact ctacaaatga agtgatccgc gaaattccac caaaacggtg     2040

gcttgatttt tatgcggcta tgactgaatt tcttgggtta tttgtagatg aaaaaaagta     2100

gaataggagt ggtttgagat ggtcacaaga ataacaggtc tggcgtcagg aatggatata     2160

gatgatatcg tatcaaagct gatgcagaca gaaagagcgc cgcttgataa gctgacacaa     2220

aaaaagcaga ctcttgaatg gcagcgtgac agctatcgtg aagtaaactc aaaaataaaa     2280

gaattgcaag attatatgtc taaaaatacg ttgacatatc cgagcacgta tcagagcaac     2340

tggcgttacc caacttaatc                                                 2360


<210> 19
<211> 3164
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 19
ctatgaccat gattacgcca agtgaacaat gatcattcat acgaagtacc atggccaaat       60

gaacataaaa gaagaacaaa tcattctttt tgaaagcggg attccaggct ttttagaaga      120

aaaacagttc gtcatacttc cgctttcaga agactctcca ttcgtggcac tgcagtccgt      180

cacttcagaa aatcttgcgt ttatcgtcgt aagtccgttt atctttttta agaattatga      240

atttgatctt gatgaatcaa ctgctgaact tttggatatc gataatattc aagacgtaga      300

agtcatgaca atattgacta tggcagagcc atttgaaaag tctactgcga atttattggc      360

tcccattatt gtgaatcgca agaacatgat ggctaagcaa gtcgttttac acgactcctc      420

atatacgaca aagcatccga ttggaggaga atcatgctag ttttatcgcg gaaaataaac      480

gaagcgattc aaataggtgc tgatattgaa gtaaaagtga ttgcggttga aggggatcaa      540

gtgaagcttg gaattgacgc cccaaagcat attgatattc acaggaaaga aatttacttg      600

accattcagg aagaaaataa ccgtgcagca gcgttatcca gcgatgtgat ctccgcatta      660

tcctcacaaa aaaagtgagg atttttttat ttttgtatta acaaaatcag agacaatccg      720

atattaatga tgtagccggg aggaggcgca aaagactcag ccagttacaa aataagggca      780

caagaacgtg ccttaacaac atattcaggg aggaacaaaa caatgaatat ggctgaaatc      840

gcccagcttg gagtctcaaa cccgtacaaa caacagtacg aaaactatat tggcggagct      900

tgggttccgc cggccggtgg cgaatacttt gaatctacaa cgccgattac gggaaaacct      960

tttacaagag ttccgcgctc cggccaacag gatgtggacg cagcgttaga tgctgcccat     1020

gcagcgaaag ctgcctgggc tagaacatca acaacggaac gcgccaatat tttaaaccgc     1080

atcgcagatc gtattgaagc gaatttgaaa ctgcttgctg tcgccgaaag cattgacaac     1140

ggaaaacctg taagagaaac aacggcagcg gatctgccgc ttgcagtgga ccattttcgt     1200

tattttgcag gttgcatcag agcacaagaa ggcggcatta gcgaaatcga tgcagacaca     1260

attgcgtacc attttcatga acctctgggt gttgtgggcc agattatccc gtggaatttt     1320

cctttattga tggcgacgtg gaaactggca ccggcgcttg ctgccggaaa ctgtgtcgta     1380

cttaaacctg cagaacaaac accggcgtct atcttagttt tgatggaagt gattggcgat     1440

ctgctgccgc cgggcgttgt gaatgtcatc aacggttttg gcttagaagc tggcaaacct     1500

ttggcctcaa gcccgcgtat ttccaaagta gcttttacgg gtgaaacaac gacaggccgg     1560

ttaatcatgc aatatgcatc acagaatttg attcctgtta cactggaact tggcggaaaa     1620

agcccgaaca ttttctttga agatgtgtta gcagcggatg acgcattttt cgacaaagcg     1680

ctggaaggat ttgcaatgtt tgcgcttaat caaggcgaag tttgcacatg tccttcacgt     1740

gctctgatcc aggaaagcat ttatgatcgg tttatggaaa gagccctgaa acgcgtggct     1800

gccatcagac aaggacatcc gcttgacacg ggaacaatga ttggtgctca agcctctgca     1860

gaacagttag aaaaaatctt gtcctacatt gatctgggca gaaaagaagg agcacagtgc     1920

cttacgggtg gcgaacgcaa tgtcctggat ggcgaccttg caggcggcta ttacgtcaaa     1980

cctacagtat ttgcgggaca taacaaaatg cgcatctttc aagaagaaat ttttggcccg     2040

gtcgtaagcg ttacgacatt taaagatgaa gaagaagcac tggctatcgc caacgacacg     2100

ttatatggat tgggtgcggg cgtttggaca agagatggag cacgtgcgtt tcggatggga     2160

agaggtattc aagctggccg cgtgtggacg aattgttatc atgcttaccc ggcccatgca     2220

gcgtttggcg gatataaaca gtctggcatc ggacgtgaaa accatcggat gatgttggat     2280

cattaccaac agacaaaaaa tttattggtt tcttactccc cgaacgcgtt gggctttttc     2340

taattttaaa aaagaccttg gcgttgccag ggtcttttaa tttaaatttc tatctcctaa     2400

tcattcctca tcctgtcact aactcatgat ataataaccg gattctccac taacttttta     2460

taaatgtatt tccatacaag aaatctaaaa cagaagattt ttttccaaaa atatgtgtaa     2520

tcttatctcg acttagtcga tataaacgat agattggggc ataggggatg atcaattgaa     2580

cattgaaagg ctcactacgt tacaacctgt ttgggatcgt tatgatactc aaatacataa     2640

tcagaaagat aatgataacg aggttcctgt tcatcaagtt tcatatacca atcttgctga     2700

aatggtgggg gaaatgaaca agcttttgga accttcgcaa gttcatctga agttcgagct     2760

tcatgacaag ttaaatgaat actatgtaaa ggtaatagag gactctacaa atgaagtgat     2820

ccgcgaaatt ccaccaaaac ggtggcttga tttttatgcg gctatgactg aatttcttgg     2880

gttatttgta gatgaaaaaa agtagaatag gagtggtttg agatggtcac aagaataaca     2940

ggtctggcgt caggaatgga tatagatgat atcgtatcaa agctgatgca gacagaaaga     3000

gcgccgcttg ataagctgac acaaaaaaag cagactcttg aatggcagcg tgacagctat     3060

cgtgaagtaa actcaaaaat aaaagaattg caagattata tgtctaaaaa tacgttgaca     3120

tatccgagca cgtatcagag caactggcgt tacccaactt aatc                      3164


<210> 20
<211> 3182
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 20
ctatgaccat gattacgcca agtgaacaat gatcattcat acgaagtacc atggccaaat       60

gaacataaaa gaagaacaaa tcattctttt tgaaagcggg attccaggct ttttagaaga      120

aaaacagttc gtcatacttc cgctttcaga agactctcca ttcgtggcac tgcagtccgt      180

cacttcagaa aatcttgcgt ttatcgtcgt aagtccgttt atctttttta agaattatga      240

atttgatctt gatgaatcaa ctgctgaact tttggatatc gataatattc aagacgtaga      300

agtcatgaca atattgacta tggcagagcc atttgaaaag tctactgcga atttattggc      360

tcccattatt gtgaatcgca agaacatgat ggctaagcaa gtcgttttac acgactcctc      420

atatacgaca aagcatccga ttggaggaga atcatgctag ttttatcgcg gaaaataaac      480

gaagcgattc aaataggtgc tgatattgaa gtaaaagtga ttgcggttga aggggatcaa      540

gtgaagcttg gaattgacgc cccaaagcat attgatattc acaggaaaga aatttacttg      600

accattcagg aagaaaataa ccgtgcagca gcgttatcca gcgatgtgat ctccgcatta      660

tcctcacaaa aaaagtgagg atttttttat ttttgtatta acaaaatcag agacaatccg      720

atattaatga tgtagccggg aggaggcgca aaagactcag ccagttacaa aataagggca      780

caagaacgtg ccttaacaac atattcaggg aggaacaaaa caatgaccaa taatccccct      840

tcagcacaga ttaagcccgg cgagtatggt ttccccctca agttaaaagc ccgctatgac      900

aactttattg gcggcgaatg ggtagcccct gccgacggcg agtattacca gaatctgacg      960

ccggtgaccg ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg     1020

gcgctggatg ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt gcaggatcgt     1080

gcggcgattc tgtttaagat tgccgatcga atggaacaaa acctcgagct gttagcgaca     1140

gctgaaacct gggataacgg caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg     1200

gcgattgacc atttccgcta tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt     1260

gaagttgata gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt ggtggggcag     1320

attatcccgt ggaacttccc gctgctgatg gcgagctgga aaatggctcc cgcgctggcg     1380

gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt actgctgcta     1440

atggaaattg tcggtgattt actgccgccg ggcgtggtga acgtggtcaa tggcgcaggt     1500

ggggtaattg gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc     1560

tcaacggaag tgggccaaca aattatgcaa tacgcaacgc aaaacattat tccggtgacg     1620

ctggagttgg gcggtaagtc gccaaatatc ttctttgctg atgtgatgga tgaagaagat     1680

gcctttttcg ataaagcgct ggaaggcttt gcactgtttg cctttaacca gggcgaagtt     1740

tgcacctgtc cgagtcgtgc tttagtgcag gaatctatct acgaacgctt tatggaacgc     1800

gccatccgcc gtgtcgaaag cattcgtagc ggtaacccgc tcgacagcgt gacgcaaatg     1860

ggcgcgcagg tttctcacgg gcaactggaa accatcctca actacattga tatcggtaaa     1920

aaagagggcg ctgacgtgct cacaggcggg cggcgcaagc tgctggaagg tgaactgaaa     1980

gacggctact acctcgaacc gacgattctg tttggtcaga acaatatgcg ggtgttccag     2040

gaggagattt ttggcccggt gctggcggtg accaccttca aaacgatgga agaagcgctg     2100

gagctggcga acgatacgca atatggcctg ggcgcgggcg tctggagccg caacggtaat     2160

ctggcctata agatggggcg cggcatacag gctgggcgcg tgtggaccaa ctgttatcac     2220

gcttacccgg cacatgcggc gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc     2280

cacaagatga tgctggagca ttaccagcaa accaagtgcc tgctggtgag ctactcggat     2340

aaaccgttgg ggctgttcta attttaaaaa agaccttggc gttgccaggg tcttttaatt     2400

taaatttcta tctcctaatc attcctcatc ctgtcactaa ctcatgatat aataaccgga     2460

ttctccacta actttttata aatgtatttc catacaagaa atctaaaaca gaagattttt     2520

ttccaaaaat atgtgtaatc ttatctcgac ttagtcgata taaacgatag attggggcat     2580

aggggatgat caattgaaca ttgaaaggct cactacgtta caacctgttt gggatcgtta     2640

tgatactcaa atacataatc agaaagataa tgataacgag gttcctgttc atcaagtttc     2700

atataccaat cttgctgaaa tggtggggga aatgaacaag cttttggaac cttcgcaagt     2760

tcatctgaag ttcgagcttc atgacaagtt aaatgaatac tatgtaaagg taatagagga     2820

ctctacaaat gaagtgatcc gcgaaattcc accaaaacgg tggcttgatt tttatgcggc     2880

tatgactgaa tttcttgggt tatttgtaga tgaaaaaaag tagaatagga gtggtttgag     2940

atggtcacaa gaataacagg tctggcgtca ggaatggata tagatgatat cgtatcaaag     3000

ctgatgcaga cagaaagagc gccgcttgat aagctgacac aaaaaaagca gactcttgaa     3060

tggcagcgtg acagctatcg tgaagtaaac tcaaaaataa aagaattgca agattatatg     3120

tctaaaaata cgttgacata tccgagcacg tatcagagca actggcgtta cccaacttaa     3180

tc                                                                    3182


<210> 21
<211> 76
<212> RNA
<213> Bacillus subtilis

<400> 21
uauaacacca auuaagagua acaaaacaag gagggacuua uacaacaauu ccgugcagga       60

acacgggaau aaaaca                                                       76


<210> 22
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 22
His His His His His His 
1               5       



