                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  CELL

<130>  P113216PCT

<150>  GB 1714718.2
<151>  2017-09-13

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  first dimerization domain sequence

<400>  1

Gln Leu Glu Lys Glu Leu Gln Ala Leu Glu Lys Glu Asn Ala Gln Leu 
1               5                   10                  15      


Glu Trp Glu Leu Gln Ala Leu Glu Lys Glu Leu Ala Gln 
            20                  25                  


<210>  2
<211>  29
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  second dimerization domain sequence

<400>  2

Gln Leu Glu Lys Lys Leu Gln Ala Leu Lys Lys Lys Asn Ala Gln Leu 
1               5                   10                  15      


Lys Trp Lys Leu Gln Ala Leu Lys Lys Lys Leu Ala Gln 
            20                  25                  


<210>  3
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  3

Gly Gly Ser Gly 
1               


<210>  4
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  4

Gly Ser Gly Ser Gly Ser 
1               5       


<210>  5
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  5

Gly Gly Ser Gly Gly Gly Ser Gly 
1               5               


<210>  6
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker sequence

<400>  6

Ser Gly Gly Ser Gly Gly Ser Gly Gly 
1               5                   


<210>  7
<211>  1590
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  constitutively active Signal Transducer and Activator of 
       Transcription (STAT) construct (STAT3 - Linker - STAT5)

<400>  7

Met Ala Gln Trp Asn Gln Leu Gln Gln Leu Asp Thr Arg Tyr Leu Glu 
1               5                   10                  15      


Gln Leu His Gln Leu Tyr Ser Asp Ser Phe Pro Met Glu Leu Arg Gln 
            20                  25                  30          


Phe Leu Ala Pro Trp Ile Glu Ser Gln Asp Trp Ala Tyr Ala Ala Ser 
        35                  40                  45              


Lys Glu Ser His Ala Thr Leu Val Phe His Asn Leu Leu Gly Glu Ile 
    50                  55                  60                  


Asp Gln Gln Tyr Ser Arg Phe Leu Gln Glu Ser Asn Val Leu Tyr Gln 
65                  70                  75                  80  


His Asn Leu Arg Arg Ile Lys Gln Phe Leu Gln Ser Arg Tyr Leu Glu 
                85                  90                  95      


Lys Pro Met Glu Ile Ala Arg Ile Val Ala Arg Cys Leu Trp Glu Glu 
            100                 105                 110         


Ser Arg Leu Leu Gln Thr Ala Ala Thr Ala Ala Gln Gln Gly Gly Gln 
        115                 120                 125             


Ala Asn His Pro Thr Ala Ala Val Val Thr Glu Lys Gln Gln Met Leu 
    130                 135                 140                 


Glu Gln His Leu Gln Asp Val Arg Lys Arg Val Gln Asp Leu Glu Gln 
145                 150                 155                 160 


Lys Met Lys Val Val Glu Asn Leu Gln Asp Asp Phe Asp Phe Asn Tyr 
                165                 170                 175     


Lys Thr Leu Lys Ser Gln Gly Asp Met Gln Asp Leu Asn Gly Asn Asn 
            180                 185                 190         


Gln Ser Val Thr Arg Gln Lys Met Gln Gln Leu Glu Gln Met Leu Thr 
        195                 200                 205             


Ala Leu Asp Gln Met Arg Arg Ser Ile Val Ser Glu Leu Ala Gly Leu 
    210                 215                 220                 


Leu Ser Ala Met Glu Tyr Val Gln Lys Thr Leu Thr Asp Glu Glu Leu 
225                 230                 235                 240 


Ala Asp Trp Lys Arg Arg Gln Gln Ile Ala Cys Ile Gly Gly Pro Pro 
                245                 250                 255     


Asn Ile Cys Leu Asp Arg Leu Glu Asn Trp Ile Thr Ser Leu Ala Glu 
            260                 265                 270         


Ser Gln Leu Gln Thr Arg Gln Gln Ile Lys Lys Leu Glu Glu Leu Gln 
        275                 280                 285             


Gln Lys Val Ser Tyr Lys Gly Asp Pro Ile Val Gln His Arg Pro Met 
    290                 295                 300                 


Leu Glu Glu Arg Ile Val Glu Leu Phe Arg Asn Leu Met Lys Ser Ala 
305                 310                 315                 320 


Phe Val Val Glu Arg Gln Pro Cys Met Pro Met His Pro Asp Arg Pro 
                325                 330                 335     


Leu Val Ile Lys Thr Gly Val Gln Phe Thr Thr Lys Val Arg Leu Leu 
            340                 345                 350         


Val Lys Phe Pro Glu Leu Asn Tyr Gln Leu Lys Ile Lys Val Cys Ile 
        355                 360                 365             


Asp Lys Asp Ser Gly Asp Val Ala Ala Leu Arg Gly Ser Arg Lys Phe 
    370                 375                 380                 


Asn Ile Leu Gly Thr Asn Thr Lys Val Met Asn Met Glu Glu Ser Asn 
385                 390                 395                 400 


Asn Gly Ser Leu Ser Ala Glu Phe Lys His Leu Thr Leu Arg Glu Gln 
                405                 410                 415     


Arg Cys Gly Asn Gly Gly Arg Ala Asn Cys Asp Ala Ser Leu Ile Val 
            420                 425                 430         


Thr Glu Glu Leu His Leu Ile Thr Phe Glu Thr Glu Val Tyr His Gln 
        435                 440                 445             


Gly Leu Lys Ile Asp Leu Glu Thr His Ser Leu Pro Val Val Val Ile 
    450                 455                 460                 


Ser Asn Ile Cys Gln Met Pro Asn Ala Trp Ala Ser Ile Leu Trp Tyr 
465                 470                 475                 480 


Asn Met Leu Thr Asn Asn Pro Lys Asn Val Asn Phe Phe Thr Lys Pro 
                485                 490                 495     


Pro Ile Gly Thr Trp Asp Gln Val Ala Glu Val Leu Ser Trp Gln Phe 
            500                 505                 510         


Ser Ser Thr Thr Lys Arg Gly Leu Ser Ile Glu Gln Leu Thr Thr Leu 
        515                 520                 525             


Ala Glu Lys Leu Leu Gly Pro Gly Val Asn Tyr Ser Gly Cys Gln Ile 
    530                 535                 540                 


Thr Trp Ala Lys Phe Cys Lys Glu Asn Met Ala Gly Lys Gly Phe Ser 
545                 550                 555                 560 


Phe Trp Val Trp Leu Asp Asn Ile Ile Asp Leu Val Lys Lys Tyr Ile 
                565                 570                 575     


Leu Ala Leu Trp Asn Glu Gly Tyr Ile Met Gly Phe Ile Ser Lys Glu 
            580                 585                 590         


Arg Glu Arg Ala Ile Leu Ser Thr Lys Pro Pro Gly Thr Phe Leu Leu 
        595                 600                 605             


Arg Phe Ser Glu Ser Ser Lys Glu Gly Gly Val Thr Phe Thr Trp Val 
    610                 615                 620                 


Glu Lys Asp Ile Ser Gly Lys Thr Gln Ile Gln Ser Val Glu Pro Tyr 
625                 630                 635                 640 


Thr Lys Gln Gln Leu Asn Asn Met Ser Phe Ala Glu Ile Ile Met Gly 
                645                 650                 655     


Tyr Lys Ile Met Asp Ala Thr Asn Ile Leu Val Ser Pro Leu Val Tyr 
            660                 665                 670         


Leu Tyr Pro Asp Ile Pro Lys Glu Glu Ala Phe Gly Lys Tyr Cys Arg 
        675                 680                 685             


Pro Glu Ser Gln Glu His Pro Glu Ala Asp Pro Gly Ser Ala Ala Pro 
    690                 695                 700                 


Tyr Leu Lys Thr Lys Phe Ile Cys Val Thr Pro Thr Thr Cys Ser Asn 
705                 710                 715                 720 


Thr Ile Asp Leu Pro Met Ser Pro Arg Thr Leu Asp Ser Leu Met Gln 
                725                 730                 735     


Phe Gly Asn Asn Gly Glu Gly Ala Glu Pro Ser Ala Gly Gly Gln Phe 
            740                 745                 750         


Glu Ser Leu Thr Phe Asp Met Glu Leu Thr Ser Glu Cys Ala Thr Ser 
        755                 760                 765             


Pro Met Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
    770                 775                 780                 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Ala Gly Trp 
785                 790                 795                 800 


Ile Gln Ala Gln Gln Leu Gln Gly Asp Ala Leu Arg Gln Met Gln Val 
                805                 810                 815     


Leu Tyr Gly Gln His Phe Pro Ile Glu Val Arg His Tyr Leu Ala Gln 
            820                 825                 830         


Trp Ile Glu Ser Gln Pro Trp Asp Ala Ile Asp Leu Asp Asn Pro Gln 
        835                 840                 845             


Asp Arg Ala Gln Ala Thr Gln Leu Leu Glu Gly Leu Val Gln Glu Leu 
    850                 855                 860                 


Gln Lys Lys Ala Glu His Gln Val Gly Glu Asp Gly Phe Leu Leu Lys 
865                 870                 875                 880 


Ile Lys Leu Gly His Tyr Ala Thr Gln Leu Gln Lys Thr Tyr Asp Arg 
                885                 890                 895     


Cys Pro Leu Glu Leu Val Arg Cys Ile Arg His Ile Leu Tyr Asn Glu 
            900                 905                 910         


Gln Arg Leu Val Arg Glu Ala Asn Asn Cys Ser Ser Pro Ala Gly Ile 
        915                 920                 925             


Leu Val Asp Ala Met Ser Gln Lys His Leu Gln Ile Asn Gln Thr Phe 
    930                 935                 940                 


Glu Glu Leu Arg Leu Val Thr Gln Asp Thr Glu Asn Glu Leu Lys Lys 
945                 950                 955                 960 


Leu Gln Gln Thr Gln Glu Tyr Phe Ile Ile Gln Tyr Gln Glu Ser Leu 
                965                 970                 975     


Arg Ile Gln Ala Gln Phe Ala Gln Leu Ala Gln Leu Ser Pro Gln Glu 
            980                 985                 990         


Arg Leu Ser Arg Glu Thr Ala Leu  Gln Gln Lys Gln Val  Ser Leu Glu 
        995                 1000                 1005             


Ala Trp  Leu Gln Arg Glu Ala  Gln Thr Leu Gln Gln  Tyr Arg Val 
    1010                 1015                 1020             


Glu Leu  Ala Glu Lys His Gln  Lys Thr Leu Gln Leu  Leu Arg Lys 
    1025                 1030                 1035             


Gln Gln  Thr Ile Ile Leu Asp  Asp Glu Leu Ile Gln  Trp Lys Arg 
    1040                 1045                 1050             


Arg Gln  Gln Leu Ala Gly Asn  Gly Gly Pro Pro Glu  Gly Ser Leu 
    1055                 1060                 1065             


Asp Val  Leu Gln Ser Trp Cys  Glu Lys Leu Ala Glu  Ile Ile Trp 
    1070                 1075                 1080             


Gln Asn  Arg Gln Gln Ile Arg  Arg Ala Glu His Leu  Cys Gln Gln 
    1085                 1090                 1095             


Leu Pro  Ile Pro Gly Pro Val  Glu Glu Met Leu Ala  Glu Val Asn 
    1100                 1105                 1110             


Ala Thr  Ile Thr Asp Ile Ile  Ser Ala Leu Val Thr  Ser Thr Phe 
    1115                 1120                 1125             


Ile Ile  Glu Lys Gln Pro Pro  Gln Val Leu Lys Thr  Gln Thr Lys 
    1130                 1135                 1140             


Phe Ala  Ala Thr Val Arg Leu  Leu Val Gly Gly Lys  Leu Asn Val 
    1145                 1150                 1155             


His Met  Asn Pro Pro Gln Val  Lys Ala Thr Ile Ile  Ser Glu Gln 
    1160                 1165                 1170             


Gln Ala  Lys Ser Leu Leu Lys  Asn Glu Asn Thr Arg  Asn Glu Cys 
    1175                 1180                 1185             


Ser Gly  Glu Ile Leu Asn Asn  Cys Cys Val Met Glu  Tyr His Gln 
    1190                 1195                 1200             


Ala Thr  Gly Thr Leu Ser Ala  His Phe Arg Asn Met  Ser Leu Lys 
    1205                 1210                 1215             


Arg Ile  Lys Arg Ala Asp Arg  Arg Gly Ala Glu Ser  Val Thr Glu 
    1220                 1225                 1230             


Glu Lys  Phe Thr Val Leu Phe  Glu Ser Gln Phe Ser  Val Gly Ser 
    1235                 1240                 1245             


Asn Glu  Leu Val Phe Gln Val  Lys Thr Leu Ser Leu  Pro Val Val 
    1250                 1255                 1260             


Val Ile  Val His Gly Ser Gln  Asp His Asn Ala Thr  Ala Thr Val 
    1265                 1270                 1275             


Leu Trp  Asp Asn Ala Phe Ala  Glu Pro Gly Arg Val  Pro Phe Ala 
    1280                 1285                 1290             


Val Pro  Asp Lys Val Leu Trp  Pro Gln Leu Cys Glu  Ala Leu Asn 
    1295                 1300                 1305             


Met Lys  Phe Lys Ala Glu Val  Gln Ser Asn Arg Gly  Leu Thr Lys 
    1310                 1315                 1320             


Glu Asn  Leu Val Phe Leu Ala  Gln Lys Leu Phe Asn  Asn Ser Ser 
    1325                 1330                 1335             


Ser His  Leu Glu Asp Tyr Ser  Gly Leu Ser Val Ser  Trp Ser Gln 
    1340                 1345                 1350             


Phe Asn  Arg Glu Asn Leu Pro  Gly Trp Asn Tyr Thr  Phe Trp Gln 
    1355                 1360                 1365             


Trp Phe  Asp Gly Val Met Glu  Val Leu Lys Lys His  His Lys Pro 
    1370                 1375                 1380             


His Trp  Asn Asp Gly Ala Ile  Leu Gly Phe Val Asn  Lys Gln Gln 
    1385                 1390                 1395             


Ala His  Asp Leu Leu Ile Asn  Lys Pro Asp Gly Thr  Phe Leu Leu 
    1400                 1405                 1410             


Arg Phe  Ser Asp Ser Glu Ile  Gly Gly Ile Thr Ile  Ala Trp Lys 
    1415                 1420                 1425             


Phe Asp  Ser Pro Glu Arg Asn  Leu Trp Asn Leu Lys  Pro Phe Thr 
    1430                 1435                 1440             


Thr Arg  Asp Phe Ser Ile Arg  Ser Leu Ala Asp Arg  Leu Gly Asp 
    1445                 1450                 1455             


Leu Ser  Tyr Leu Ile Tyr Val  Phe Pro Asp Arg Pro  Lys Asp Glu 
    1460                 1465                 1470             


Val Phe  Ser Lys Tyr Tyr Thr  Pro Val Leu Ala Lys  Ala Val Asp 
    1475                 1480                 1485             


Gly Tyr  Val Lys Pro Gln Ile  Lys Gln Val Val Pro  Glu Phe Val 
    1490                 1495                 1500             


Asn Ala  Ser Ala Asp Ala Gly  Gly Ser Ser Ala Thr  Tyr Met Asp 
    1505                 1510                 1515             


Gln Ala  Pro Ser Pro Ala Val  Cys Pro Gln Ala Pro  Tyr Asn Met 
    1520                 1525                 1530             


Tyr Pro  Gln Asn Pro Asp His  Val Leu Asp Gln Asp  Gly Glu Phe 
    1535                 1540                 1545             


Asp Leu  Asp Glu Thr Met Asp  Val Ala Arg His Val  Glu Glu Leu 
    1550                 1555                 1560             


Leu Arg  Arg Pro Met Asp Ser  Leu Asp Ser Arg Leu  Ser Pro Pro 
    1565                 1570                 1575             


Ala Gly  Leu Phe Thr Ser Ala  Arg Gly Ser Leu Ser  
    1580                 1585                 1590 


<210>  8
<211>  1896
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  constitutively active STAT construct (TIP - STAT3 - Linker - 
       STAT5 - 2a - Myristoylation and palmitoylation sequence - Ridge 
       Linker - TetRB)

<400>  8

Met Trp Thr Trp Asn Ala Tyr Ala Phe Ala Ala Pro Ser Gly Gly Gly 
1               5                   10                  15      


Ser Ala Gln Trp Asn Gln Leu Gln Gln Leu Asp Thr Arg Tyr Leu Glu 
            20                  25                  30          


Gln Leu His Gln Leu Tyr Ser Asp Ser Phe Pro Met Glu Leu Arg Gln 
        35                  40                  45              


Phe Leu Ala Pro Trp Ile Glu Ser Gln Asp Trp Ala Tyr Ala Ala Ser 
    50                  55                  60                  


Lys Glu Ser His Ala Thr Leu Val Phe His Asn Leu Leu Gly Glu Ile 
65                  70                  75                  80  


Asp Gln Gln Tyr Ser Arg Phe Leu Gln Glu Ser Asn Val Leu Tyr Gln 
                85                  90                  95      


His Asn Leu Arg Arg Ile Lys Gln Phe Leu Gln Ser Arg Tyr Leu Glu 
            100                 105                 110         


Lys Pro Met Glu Ile Ala Arg Ile Val Ala Arg Cys Leu Trp Glu Glu 
        115                 120                 125             


Ser Arg Leu Leu Gln Thr Ala Ala Thr Ala Ala Gln Gln Gly Gly Gln 
    130                 135                 140                 


Ala Asn His Pro Thr Ala Ala Val Val Thr Glu Lys Gln Gln Met Leu 
145                 150                 155                 160 


Glu Gln His Leu Gln Asp Val Arg Lys Arg Val Gln Asp Leu Glu Gln 
                165                 170                 175     


Lys Met Lys Val Val Glu Asn Leu Gln Asp Asp Phe Asp Phe Asn Tyr 
            180                 185                 190         


Lys Thr Leu Lys Ser Gln Gly Asp Met Gln Asp Leu Asn Gly Asn Asn 
        195                 200                 205             


Gln Ser Val Thr Arg Gln Lys Met Gln Gln Leu Glu Gln Met Leu Thr 
    210                 215                 220                 


Ala Leu Asp Gln Met Arg Arg Ser Ile Val Ser Glu Leu Ala Gly Leu 
225                 230                 235                 240 


Leu Ser Ala Met Glu Tyr Val Gln Lys Thr Leu Thr Asp Glu Glu Leu 
                245                 250                 255     


Ala Asp Trp Lys Arg Arg Gln Gln Ile Ala Cys Ile Gly Gly Pro Pro 
            260                 265                 270         


Asn Ile Cys Leu Asp Arg Leu Glu Asn Trp Ile Thr Ser Leu Ala Glu 
        275                 280                 285             


Ser Gln Leu Gln Thr Arg Gln Gln Ile Lys Lys Leu Glu Glu Leu Gln 
    290                 295                 300                 


Gln Lys Val Ser Tyr Lys Gly Asp Pro Ile Val Gln His Arg Pro Met 
305                 310                 315                 320 


Leu Glu Glu Arg Ile Val Glu Leu Phe Arg Asn Leu Met Lys Ser Ala 
                325                 330                 335     


Phe Val Val Glu Arg Gln Pro Cys Met Pro Met His Pro Asp Arg Pro 
            340                 345                 350         


Leu Val Ile Lys Thr Gly Val Gln Phe Thr Thr Lys Val Arg Leu Leu 
        355                 360                 365             


Val Lys Phe Pro Glu Leu Asn Tyr Gln Leu Lys Ile Lys Val Cys Ile 
    370                 375                 380                 


Asp Lys Asp Ser Gly Asp Val Ala Ala Leu Arg Gly Ser Arg Lys Phe 
385                 390                 395                 400 


Asn Ile Leu Gly Thr Asn Thr Lys Val Met Asn Met Glu Glu Ser Asn 
                405                 410                 415     


Asn Gly Ser Leu Ser Ala Glu Phe Lys His Leu Thr Leu Arg Glu Gln 
            420                 425                 430         


Arg Cys Gly Asn Gly Gly Arg Ala Asn Cys Asp Ala Ser Leu Ile Val 
        435                 440                 445             


Thr Glu Glu Leu His Leu Ile Thr Phe Glu Thr Glu Val Tyr His Gln 
    450                 455                 460                 


Gly Leu Lys Ile Asp Leu Glu Thr His Ser Leu Pro Val Val Val Ile 
465                 470                 475                 480 


Ser Asn Ile Cys Gln Met Pro Asn Ala Trp Ala Ser Ile Leu Trp Tyr 
                485                 490                 495     


Asn Met Leu Thr Asn Asn Pro Lys Asn Val Asn Phe Phe Thr Lys Pro 
            500                 505                 510         


Pro Ile Gly Thr Trp Asp Gln Val Ala Glu Val Leu Ser Trp Gln Phe 
        515                 520                 525             


Ser Ser Thr Thr Lys Arg Gly Leu Ser Ile Glu Gln Leu Thr Thr Leu 
    530                 535                 540                 


Ala Glu Lys Leu Leu Gly Pro Gly Val Asn Tyr Ser Gly Cys Gln Ile 
545                 550                 555                 560 


Thr Trp Ala Lys Phe Cys Lys Glu Asn Met Ala Gly Lys Gly Phe Ser 
                565                 570                 575     


Phe Trp Val Trp Leu Asp Asn Ile Ile Asp Leu Val Lys Lys Tyr Ile 
            580                 585                 590         


Leu Ala Leu Trp Asn Glu Gly Tyr Ile Met Gly Phe Ile Ser Lys Glu 
        595                 600                 605             


Arg Glu Arg Ala Ile Leu Ser Thr Lys Pro Pro Gly Thr Phe Leu Leu 
    610                 615                 620                 


Arg Phe Ser Glu Ser Ser Lys Glu Gly Gly Val Thr Phe Thr Trp Val 
625                 630                 635                 640 


Glu Lys Asp Ile Ser Gly Lys Thr Gln Ile Gln Ser Val Glu Pro Tyr 
                645                 650                 655     


Thr Lys Gln Gln Leu Asn Asn Met Ser Phe Ala Glu Ile Ile Met Gly 
            660                 665                 670         


Tyr Lys Ile Met Asp Ala Thr Asn Ile Leu Val Ser Pro Leu Val Tyr 
        675                 680                 685             


Leu Tyr Pro Asp Ile Pro Lys Glu Glu Ala Phe Gly Lys Tyr Cys Arg 
    690                 695                 700                 


Pro Glu Ser Gln Glu His Pro Glu Ala Asp Pro Gly Ser Ala Ala Pro 
705                 710                 715                 720 


Tyr Leu Lys Thr Lys Phe Ile Cys Val Thr Pro Thr Thr Cys Ser Asn 
                725                 730                 735     


Thr Ile Asp Leu Pro Met Ser Pro Arg Thr Leu Asp Ser Leu Met Gln 
            740                 745                 750         


Phe Gly Asn Asn Gly Glu Gly Ala Glu Pro Ser Ala Gly Gly Gln Phe 
        755                 760                 765             


Glu Ser Leu Thr Phe Asp Met Glu Leu Thr Ser Glu Cys Ala Thr Ser 
    770                 775                 780                 


Pro Met Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
785                 790                 795                 800 


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Ala Gly Trp 
                805                 810                 815     


Ile Gln Ala Gln Gln Leu Gln Gly Asp Ala Leu Arg Gln Met Gln Val 
            820                 825                 830         


Leu Tyr Gly Gln His Phe Pro Ile Glu Val Arg His Tyr Leu Ala Gln 
        835                 840                 845             


Trp Ile Glu Ser Gln Pro Trp Asp Ala Ile Asp Leu Asp Asn Pro Gln 
    850                 855                 860                 


Asp Arg Ala Gln Ala Thr Gln Leu Leu Glu Gly Leu Val Gln Glu Leu 
865                 870                 875                 880 


Gln Lys Lys Ala Glu His Gln Val Gly Glu Asp Gly Phe Leu Leu Lys 
                885                 890                 895     


Ile Lys Leu Gly His Tyr Ala Thr Gln Leu Gln Lys Thr Tyr Asp Arg 
            900                 905                 910         


Cys Pro Leu Glu Leu Val Arg Cys Ile Arg His Ile Leu Tyr Asn Glu 
        915                 920                 925             


Gln Arg Leu Val Arg Glu Ala Asn Asn Cys Ser Ser Pro Ala Gly Ile 
    930                 935                 940                 


Leu Val Asp Ala Met Ser Gln Lys His Leu Gln Ile Asn Gln Thr Phe 
945                 950                 955                 960 


Glu Glu Leu Arg Leu Val Thr Gln Asp Thr Glu Asn Glu Leu Lys Lys 
                965                 970                 975     


Leu Gln Gln Thr Gln Glu Tyr Phe Ile Ile Gln Tyr Gln Glu Ser Leu 
            980                 985                 990         


Arg Ile Gln Ala Gln Phe Ala Gln  Leu Ala Gln Leu Ser  Pro Gln Glu 
        995                 1000                 1005             


Arg Leu  Ser Arg Glu Thr Ala  Leu Gln Gln Lys Gln  Val Ser Leu 
    1010                 1015                 1020             


Glu Ala  Trp Leu Gln Arg Glu  Ala Gln Thr Leu Gln  Gln Tyr Arg 
    1025                 1030                 1035             


Val Glu  Leu Ala Glu Lys His  Gln Lys Thr Leu Gln  Leu Leu Arg 
    1040                 1045                 1050             


Lys Gln  Gln Thr Ile Ile Leu  Asp Asp Glu Leu Ile  Gln Trp Lys 
    1055                 1060                 1065             


Arg Arg  Gln Gln Leu Ala Gly  Asn Gly Gly Pro Pro  Glu Gly Ser 
    1070                 1075                 1080             


Leu Asp  Val Leu Gln Ser Trp  Cys Glu Lys Leu Ala  Glu Ile Ile 
    1085                 1090                 1095             


Trp Gln  Asn Arg Gln Gln Ile  Arg Arg Ala Glu His  Leu Cys Gln 
    1100                 1105                 1110             


Gln Leu  Pro Ile Pro Gly Pro  Val Glu Glu Met Leu  Ala Glu Val 
    1115                 1120                 1125             


Asn Ala  Thr Ile Thr Asp Ile  Ile Ser Ala Leu Val  Thr Ser Thr 
    1130                 1135                 1140             


Phe Ile  Ile Glu Lys Gln Pro  Pro Gln Val Leu Lys  Thr Gln Thr 
    1145                 1150                 1155             


Lys Phe  Ala Ala Thr Val Arg  Leu Leu Val Gly Gly  Lys Leu Asn 
    1160                 1165                 1170             


Val His  Met Asn Pro Pro Gln  Val Lys Ala Thr Ile  Ile Ser Glu 
    1175                 1180                 1185             


Gln Gln  Ala Lys Ser Leu Leu  Lys Asn Glu Asn Thr  Arg Asn Glu 
    1190                 1195                 1200             


Cys Ser  Gly Glu Ile Leu Asn  Asn Cys Cys Val Met  Glu Tyr His 
    1205                 1210                 1215             


Gln Ala  Thr Gly Thr Leu Ser  Ala His Phe Arg Asn  Met Ser Leu 
    1220                 1225                 1230             


Lys Arg  Ile Lys Arg Ala Asp  Arg Arg Gly Ala Glu  Ser Val Thr 
    1235                 1240                 1245             


Glu Glu  Lys Phe Thr Val Leu  Phe Glu Ser Gln Phe  Ser Val Gly 
    1250                 1255                 1260             


Ser Asn  Glu Leu Val Phe Gln  Val Lys Thr Leu Ser  Leu Pro Val 
    1265                 1270                 1275             


Val Val  Ile Val His Gly Ser  Gln Asp His Asn Ala  Thr Ala Thr 
    1280                 1285                 1290             


Val Leu  Trp Asp Asn Ala Phe  Ala Glu Pro Gly Arg  Val Pro Phe 
    1295                 1300                 1305             


Ala Val  Pro Asp Lys Val Leu  Trp Pro Gln Leu Cys  Glu Ala Leu 
    1310                 1315                 1320             


Asn Met  Lys Phe Lys Ala Glu  Val Gln Ser Asn Arg  Gly Leu Thr 
    1325                 1330                 1335             


Lys Glu  Asn Leu Val Phe Leu  Ala Gln Lys Leu Phe  Asn Asn Ser 
    1340                 1345                 1350             


Ser Ser  His Leu Glu Asp Tyr  Ser Gly Leu Ser Val  Ser Trp Ser 
    1355                 1360                 1365             


Gln Phe  Asn Arg Glu Asn Leu  Pro Gly Trp Asn Tyr  Thr Phe Trp 
    1370                 1375                 1380             


Gln Trp  Phe Asp Gly Val Met  Glu Val Leu Lys Lys  His His Lys 
    1385                 1390                 1395             


Pro His  Trp Asn Asp Gly Ala  Ile Leu Gly Phe Val  Asn Lys Gln 
    1400                 1405                 1410             


Gln Ala  His Asp Leu Leu Ile  Asn Lys Pro Asp Gly  Thr Phe Leu 
    1415                 1420                 1425             


Leu Arg  Phe Ser Asp Ser Glu  Ile Gly Gly Ile Thr  Ile Ala Trp 
    1430                 1435                 1440             


Lys Phe  Asp Ser Pro Glu Arg  Asn Leu Trp Asn Leu  Lys Pro Phe 
    1445                 1450                 1455             


Thr Thr  Arg Asp Phe Ser Ile  Arg Ser Leu Ala Asp  Arg Leu Gly 
    1460                 1465                 1470             


Asp Leu  Ser Tyr Leu Ile Tyr  Val Phe Pro Asp Arg  Pro Lys Asp 
    1475                 1480                 1485             


Glu Val  Phe Ser Lys Tyr Tyr  Thr Pro Val Leu Ala  Lys Ala Val 
    1490                 1495                 1500             


Asp Gly  Tyr Val Lys Pro Gln  Ile Lys Gln Val Val  Pro Glu Phe 
    1505                 1510                 1515             


Val Asn  Ala Ser Ala Asp Ala  Gly Gly Ser Ser Ala  Thr Tyr Met 
    1520                 1525                 1530             


Asp Gln  Ala Pro Ser Pro Ala  Val Cys Pro Gln Ala  Pro Tyr Asn 
    1535                 1540                 1545             


Met Tyr  Pro Gln Asn Pro Asp  His Val Leu Asp Gln  Asp Gly Glu 
    1550                 1555                 1560             


Phe Asp  Leu Asp Glu Thr Met  Asp Val Ala Arg His  Val Glu Glu 
    1565                 1570                 1575             


Leu Leu  Arg Arg Pro Met Asp  Ser Leu Asp Ser Arg  Leu Ser Pro 
    1580                 1585                 1590             


Pro Ala  Gly Leu Phe Thr Ser  Ala Arg Gly Ser Leu  Ser Glu Gly 
    1595                 1600                 1605             


Arg Gly  Ser Leu Leu Thr Cys  Gly Asp Val Glu Glu  Asn Pro Gly 
    1610                 1615                 1620             


Pro Met  Gly Cys Gly Cys Ser  Ser His Pro Glu Leu  Glu Ala Glu 
    1625                 1630                 1635             


Ala Ala  Ala Lys Glu Ala Ala  Ala Lys Glu Ala Ala  Ala Lys Glu 
    1640                 1645                 1650             


Ala Ala  Ala Lys Ala Leu Glu  Ala Glu Ala Ala Ala  Lys Glu Ala 
    1655                 1660                 1665             


Ala Ala  Lys Glu Ala Ala Ala  Lys Glu Ala Ala Ala  Lys Ala Leu 
    1670                 1675                 1680             


Glu Ser  Gly Gly Gly Ser Met  Ser Arg Leu Asp Lys  Ser Lys Val 
    1685                 1690                 1695             


Ile Asn  Ser Ala Leu Glu Leu  Leu Asn Glu Val Gly  Ile Glu Gly 
    1700                 1705                 1710             


Leu Thr  Thr Arg Lys Leu Ala  Gln Lys Leu Gly Val  Glu Gln Pro 
    1715                 1720                 1725             


Thr Leu  Tyr Trp His Val Lys  Asn Lys Arg Ala Leu  Leu Asp Ala 
    1730                 1735                 1740             


Leu Ala  Ile Glu Met Leu Asp  Arg His His Thr His  Phe Cys Pro 
    1745                 1750                 1755             


Leu Glu  Gly Glu Ser Trp Gln  Asp Phe Leu Arg Asn  Asn Ala Lys 
    1760                 1765                 1770             


Ser Phe  Arg Cys Ala Leu Leu  Ser His Arg Asp Gly  Ala Lys Val 
    1775                 1780                 1785             


His Leu  Gly Thr Arg Pro Thr  Glu Lys Gln Tyr Glu  Thr Leu Glu 
    1790                 1795                 1800             


Asn Gln  Leu Ala Phe Leu Cys  Gln Gln Gly Phe Ser  Leu Glu Asn 
    1805                 1810                 1815             


Ala Leu  Tyr Ala Leu Ser Ala  Val Gly His Phe Thr  Leu Gly Cys 
    1820                 1825                 1830             


Val Leu  Glu Asp Gln Glu His  Gln Val Ala Lys Glu  Glu Arg Glu 
    1835                 1840                 1845             


Thr Pro  Thr Thr Asp Ser Met  Pro Pro Leu Leu Arg  Gln Ala Ile 
    1850                 1855                 1860             


Glu Leu  Phe Asp His Gln Gly  Ala Glu Pro Ala Phe  Leu Phe Gly 
    1865                 1870                 1875             


Leu Glu  Leu Ile Ile Cys Gly  Leu Glu Lys Gln Leu  Lys Cys Glu 
    1880                 1885                 1890             


Ser Gly  Ser 
    1895     


<210>  9
<211>  2729
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  constitutively active STAT construct (SH2 ZAP70 - Linker - TEV - 
       2a - myristoylation and palmitoylation sequence - linker - TEV 
       cleavage site x3 - STAT3 - Linker -STAT5 - 2a - aCD19)

<400>  9

Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
1               5                   10                  15      


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
            20                  25                  30          


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
        35                  40                  45              


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
    50                  55                  60                  


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
65                  70                  75                  80  


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
                85                  90                  95      


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
            100                 105                 110         


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
        115                 120                 125             


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
    130                 135                 140                 


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
145                 150                 155                 160 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
                165                 170                 175     


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg 
            180                 185                 190         


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
        195                 200                 205             


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
    210                 215                 220                 


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
225                 230                 235                 240 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
                245                 250                 255     


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
            260                 265                 270         


His Pro Ser Thr Leu Thr His Pro Ser Gly Gly Gly Gly Ser Ser Leu 
        275                 280                 285             


Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser Thr Ile Cys His 
    290                 295                 300                 


Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile Gly 
305                 310                 315                 320 


Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe Arg Arg Asn Asn 
                325                 330                 335     


Gly Thr Leu Leu Val Gln Ser Leu His Gly Val Phe Lys Val Lys Asn 
            340                 345                 350         


Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg Asp Met Ile Ile 
        355                 360                 365             


Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys Phe 
    370                 375                 380                 


Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn Phe 
385                 390                 395                 400 


Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr Phe 
                405                 410                 415     


Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys Asp 
            420                 425                 430         


Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile Val 
        435                 440                 445             


Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe Thr 
    450                 455                 460                 


Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala Gln 
465                 470                 475                 480 


Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp Gly 
                485                 490                 495     


Gly His Lys Val Phe Met Ser Lys Pro Glu Glu Pro Phe Gln Pro Val 
            500                 505                 510         


Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr Ser Gln Glu Gly 
        515                 520                 525             


Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly Pro 
    530                 535                 540                 


Met Gly Cys Gly Cys Ser Ser His Pro Glu Ser Gly Gly Gly Gly Ser 
545                 550                 555                 560 


Gly Gly Gly Gly Ser Glu Asn Leu Tyr Phe Gln Gly Glu Asn Leu Tyr 
                565                 570                 575     


Phe Gln Gly Glu Asn Leu Tyr Phe Gln Gly Glu Asn Leu Tyr Phe Gln 
            580                 585                 590         


Gly Glu Asn Leu Tyr Phe Gln Gly Glu Asn Leu Tyr Phe Gln Gly Glu 
        595                 600                 605             


Asn Leu Tyr Phe Gln Gly Glu Asn Leu Tyr Phe Gln Gly Glu Asn Leu 
    610                 615                 620                 


Tyr Phe Gln Gly Ala Gln Trp Asn Gln Leu Gln Gln Leu Asp Thr Arg 
625                 630                 635                 640 


Tyr Leu Glu Gln Leu His Gln Leu Tyr Ser Asp Ser Phe Pro Met Glu 
                645                 650                 655     


Leu Arg Gln Phe Leu Ala Pro Trp Ile Glu Ser Gln Asp Trp Ala Tyr 
            660                 665                 670         


Ala Ala Ser Lys Glu Ser His Ala Thr Leu Val Phe His Asn Leu Leu 
        675                 680                 685             


Gly Glu Ile Asp Gln Gln Tyr Ser Arg Phe Leu Gln Glu Ser Asn Val 
    690                 695                 700                 


Leu Tyr Gln His Asn Leu Arg Arg Ile Lys Gln Phe Leu Gln Ser Arg 
705                 710                 715                 720 


Tyr Leu Glu Lys Pro Met Glu Ile Ala Arg Ile Val Ala Arg Cys Leu 
                725                 730                 735     


Trp Glu Glu Ser Arg Leu Leu Gln Thr Ala Ala Thr Ala Ala Gln Gln 
            740                 745                 750         


Gly Gly Gln Ala Asn His Pro Thr Ala Ala Val Val Thr Glu Lys Gln 
        755                 760                 765             


Gln Met Leu Glu Gln His Leu Gln Asp Val Arg Lys Arg Val Gln Asp 
    770                 775                 780                 


Leu Glu Gln Lys Met Lys Val Val Glu Asn Leu Gln Asp Asp Phe Asp 
785                 790                 795                 800 


Phe Asn Tyr Lys Thr Leu Lys Ser Gln Gly Asp Met Gln Asp Leu Asn 
                805                 810                 815     


Gly Asn Asn Gln Ser Val Thr Arg Gln Lys Met Gln Gln Leu Glu Gln 
            820                 825                 830         


Met Leu Thr Ala Leu Asp Gln Met Arg Arg Ser Ile Val Ser Glu Leu 
        835                 840                 845             


Ala Gly Leu Leu Ser Ala Met Glu Tyr Val Gln Lys Thr Leu Thr Asp 
    850                 855                 860                 


Glu Glu Leu Ala Asp Trp Lys Arg Arg Gln Gln Ile Ala Cys Ile Gly 
865                 870                 875                 880 


Gly Pro Pro Asn Ile Cys Leu Asp Arg Leu Glu Asn Trp Ile Thr Ser 
                885                 890                 895     


Leu Ala Glu Ser Gln Leu Gln Thr Arg Gln Gln Ile Lys Lys Leu Glu 
            900                 905                 910         


Glu Leu Gln Gln Lys Val Ser Tyr Lys Gly Asp Pro Ile Val Gln His 
        915                 920                 925             


Arg Pro Met Leu Glu Glu Arg Ile Val Glu Leu Phe Arg Asn Leu Met 
    930                 935                 940                 


Lys Ser Ala Phe Val Val Glu Arg Gln Pro Cys Met Pro Met His Pro 
945                 950                 955                 960 


Asp Arg Pro Leu Val Ile Lys Thr Gly Val Gln Phe Thr Thr Lys Val 
                965                 970                 975     


Arg Leu Leu Val Lys Phe Pro Glu Leu Asn Tyr Gln Leu Lys Ile Lys 
            980                 985                 990         


Val Cys Ile Asp Lys Asp Ser Gly  Asp Val Ala Ala Leu  Arg Gly Ser 
        995                 1000                 1005             


Arg Lys  Phe Asn Ile Leu Gly  Thr Asn Thr Lys Val  Met Asn Met 
    1010                 1015                 1020             


Glu Glu  Ser Asn Asn Gly Ser  Leu Ser Ala Glu Phe  Lys His Leu 
    1025                 1030                 1035             


Thr Leu  Arg Glu Gln Arg Cys  Gly Asn Gly Gly Arg  Ala Asn Cys 
    1040                 1045                 1050             


Asp Ala  Ser Leu Ile Val Thr  Glu Glu Leu His Leu  Ile Thr Phe 
    1055                 1060                 1065             


Glu Thr  Glu Val Tyr His Gln  Gly Leu Lys Ile Asp  Leu Glu Thr 
    1070                 1075                 1080             


His Ser  Leu Pro Val Val Val  Ile Ser Asn Ile Cys  Gln Met Pro 
    1085                 1090                 1095             


Asn Ala  Trp Ala Ser Ile Leu  Trp Tyr Asn Met Leu  Thr Asn Asn 
    1100                 1105                 1110             


Pro Lys  Asn Val Asn Phe Phe  Thr Lys Pro Pro Ile  Gly Thr Trp 
    1115                 1120                 1125             


Asp Gln  Val Ala Glu Val Leu  Ser Trp Gln Phe Ser  Ser Thr Thr 
    1130                 1135                 1140             


Lys Arg  Gly Leu Ser Ile Glu  Gln Leu Thr Thr Leu  Ala Glu Lys 
    1145                 1150                 1155             


Leu Leu  Gly Pro Gly Val Asn  Tyr Ser Gly Cys Gln  Ile Thr Trp 
    1160                 1165                 1170             


Ala Lys  Phe Cys Lys Glu Asn  Met Ala Gly Lys Gly  Phe Ser Phe 
    1175                 1180                 1185             


Trp Val  Trp Leu Asp Asn Ile  Ile Asp Leu Val Lys  Lys Tyr Ile 
    1190                 1195                 1200             


Leu Ala  Leu Trp Asn Glu Gly  Tyr Ile Met Gly Phe  Ile Ser Lys 
    1205                 1210                 1215             


Glu Arg  Glu Arg Ala Ile Leu  Ser Thr Lys Pro Pro  Gly Thr Phe 
    1220                 1225                 1230             


Leu Leu  Arg Phe Ser Glu Ser  Ser Lys Glu Gly Gly  Val Thr Phe 
    1235                 1240                 1245             


Thr Trp  Val Glu Lys Asp Ile  Ser Gly Lys Thr Gln  Ile Gln Ser 
    1250                 1255                 1260             


Val Glu  Pro Tyr Thr Lys Gln  Gln Leu Asn Asn Met  Ser Phe Ala 
    1265                 1270                 1275             


Glu Ile  Ile Met Gly Tyr Lys  Ile Met Asp Ala Thr  Asn Ile Leu 
    1280                 1285                 1290             


Val Ser  Pro Leu Val Tyr Leu  Tyr Pro Asp Ile Pro  Lys Glu Glu 
    1295                 1300                 1305             


Ala Phe  Gly Lys Tyr Cys Arg  Pro Glu Ser Gln Glu  His Pro Glu 
    1310                 1315                 1320             


Ala Asp  Pro Gly Ser Ala Ala  Pro Tyr Leu Lys Thr  Lys Phe Ile 
    1325                 1330                 1335             


Cys Val  Thr Pro Thr Thr Cys  Ser Asn Thr Ile Asp  Leu Pro Met 
    1340                 1345                 1350             


Ser Pro  Arg Thr Leu Asp Ser  Leu Met Gln Phe Gly  Asn Asn Gly 
    1355                 1360                 1365             


Glu Gly  Ala Glu Pro Ser Ala  Gly Gly Gln Phe Glu  Ser Leu Thr 
    1370                 1375                 1380             


Phe Asp  Met Glu Leu Thr Ser  Glu Cys Ala Thr Ser  Pro Met Ser 
    1385                 1390                 1395             


Gly Gly  Gly Gly Ser Gly Gly  Gly Gly Ser Gly Gly  Gly Gly Ser 
    1400                 1405                 1410             


Gly Gly  Gly Gly Ser Gly Gly  Gly Gly Ser Met Ala  Gly Trp Ile 
    1415                 1420                 1425             


Gln Ala  Gln Gln Leu Gln Gly  Asp Ala Leu Arg Gln  Met Gln Val 
    1430                 1435                 1440             


Leu Tyr  Gly Gln His Phe Pro  Ile Glu Val Arg His  Tyr Leu Ala 
    1445                 1450                 1455             


Gln Trp  Ile Glu Ser Gln Pro  Trp Asp Ala Ile Asp  Leu Asp Asn 
    1460                 1465                 1470             


Pro Gln  Asp Arg Ala Gln Ala  Thr Gln Leu Leu Glu  Gly Leu Val 
    1475                 1480                 1485             


Gln Glu  Leu Gln Lys Lys Ala  Glu His Gln Val Gly  Glu Asp Gly 
    1490                 1495                 1500             


Phe Leu  Leu Lys Ile Lys Leu  Gly His Tyr Ala Thr  Gln Leu Gln 
    1505                 1510                 1515             


Lys Thr  Tyr Asp Arg Cys Pro  Leu Glu Leu Val Arg  Cys Ile Arg 
    1520                 1525                 1530             


His Ile  Leu Tyr Asn Glu Gln  Arg Leu Val Arg Glu  Ala Asn Asn 
    1535                 1540                 1545             


Cys Ser  Ser Pro Ala Gly Ile  Leu Val Asp Ala Met  Ser Gln Lys 
    1550                 1555                 1560             


His Leu  Gln Ile Asn Gln Thr  Phe Glu Glu Leu Arg  Leu Val Thr 
    1565                 1570                 1575             


Gln Asp  Thr Glu Asn Glu Leu  Lys Lys Leu Gln Gln  Thr Gln Glu 
    1580                 1585                 1590             


Tyr Phe  Ile Ile Gln Tyr Gln  Glu Ser Leu Arg Ile  Gln Ala Gln 
    1595                 1600                 1605             


Phe Ala  Gln Leu Ala Gln Leu  Ser Pro Gln Glu Arg  Leu Ser Arg 
    1610                 1615                 1620             


Glu Thr  Ala Leu Gln Gln Lys  Gln Val Ser Leu Glu  Ala Trp Leu 
    1625                 1630                 1635             


Gln Arg  Glu Ala Gln Thr Leu  Gln Gln Tyr Arg Val  Glu Leu Ala 
    1640                 1645                 1650             


Glu Lys  His Gln Lys Thr Leu  Gln Leu Leu Arg Lys  Gln Gln Thr 
    1655                 1660                 1665             


Ile Ile  Leu Asp Asp Glu Leu  Ile Gln Trp Lys Arg  Arg Gln Gln 
    1670                 1675                 1680             


Leu Ala  Gly Asn Gly Gly Pro  Pro Glu Gly Ser Leu  Asp Val Leu 
    1685                 1690                 1695             


Gln Ser  Trp Cys Glu Lys Leu  Ala Glu Ile Ile Trp  Gln Asn Arg 
    1700                 1705                 1710             


Gln Gln  Ile Arg Arg Ala Glu  His Leu Cys Gln Gln  Leu Pro Ile 
    1715                 1720                 1725             


Pro Gly  Pro Val Glu Glu Met  Leu Ala Glu Val Asn  Ala Thr Ile 
    1730                 1735                 1740             


Thr Asp  Ile Ile Ser Ala Leu  Val Thr Ser Thr Phe  Ile Ile Glu 
    1745                 1750                 1755             


Lys Gln  Pro Pro Gln Val Leu  Lys Thr Gln Thr Lys  Phe Ala Ala 
    1760                 1765                 1770             


Thr Val  Arg Leu Leu Val Gly  Gly Lys Leu Asn Val  His Met Asn 
    1775                 1780                 1785             


Pro Pro  Gln Val Lys Ala Thr  Ile Ile Ser Glu Gln  Gln Ala Lys 
    1790                 1795                 1800             


Ser Leu  Leu Lys Asn Glu Asn  Thr Arg Asn Glu Cys  Ser Gly Glu 
    1805                 1810                 1815             


Ile Leu  Asn Asn Cys Cys Val  Met Glu Tyr His Gln  Ala Thr Gly 
    1820                 1825                 1830             


Thr Leu  Ser Ala His Phe Arg  Asn Met Ser Leu Lys  Arg Ile Lys 
    1835                 1840                 1845             


Arg Ala  Asp Arg Arg Gly Ala  Glu Ser Val Thr Glu  Glu Lys Phe 
    1850                 1855                 1860             


Thr Val  Leu Phe Glu Ser Gln  Phe Ser Val Gly Ser  Asn Glu Leu 
    1865                 1870                 1875             


Val Phe  Gln Val Lys Thr Leu  Ser Leu Pro Val Val  Val Ile Val 
    1880                 1885                 1890             


His Gly  Ser Gln Asp His Asn  Ala Thr Ala Thr Val  Leu Trp Asp 
    1895                 1900                 1905             


Asn Ala  Phe Ala Glu Pro Gly  Arg Val Pro Phe Ala  Val Pro Asp 
    1910                 1915                 1920             


Lys Val  Leu Trp Pro Gln Leu  Cys Glu Ala Leu Asn  Met Lys Phe 
    1925                 1930                 1935             


Lys Ala  Glu Val Gln Ser Asn  Arg Gly Leu Thr Lys  Glu Asn Leu 
    1940                 1945                 1950             


Val Phe  Leu Ala Gln Lys Leu  Phe Asn Asn Ser Ser  Ser His Leu 
    1955                 1960                 1965             


Glu Asp  Tyr Ser Gly Leu Ser  Val Ser Trp Ser Gln  Phe Asn Arg 
    1970                 1975                 1980             


Glu Asn  Leu Pro Gly Trp Asn  Tyr Thr Phe Trp Gln  Trp Phe Asp 
    1985                 1990                 1995             


Gly Val  Met Glu Val Leu Lys  Lys His His Lys Pro  His Trp Asn 
    2000                 2005                 2010             


Asp Gly  Ala Ile Leu Gly Phe  Val Asn Lys Gln Gln  Ala His Asp 
    2015                 2020                 2025             


Leu Leu  Ile Asn Lys Pro Asp  Gly Thr Phe Leu Leu  Arg Phe Ser 
    2030                 2035                 2040             


Asp Ser  Glu Ile Gly Gly Ile  Thr Ile Ala Trp Lys  Phe Asp Ser 
    2045                 2050                 2055             


Pro Glu  Arg Asn Leu Trp Asn  Leu Lys Pro Phe Thr  Thr Arg Asp 
    2060                 2065                 2070             


Phe Ser  Ile Arg Ser Leu Ala  Asp Arg Leu Gly Asp  Leu Ser Tyr 
    2075                 2080                 2085             


Leu Ile  Tyr Val Phe Pro Asp  Arg Pro Lys Asp Glu  Val Phe Ser 
    2090                 2095                 2100             


Lys Tyr  Tyr Thr Pro Val Leu  Ala Lys Ala Val Asp  Gly Tyr Val 
    2105                 2110                 2115             


Lys Pro  Gln Ile Lys Gln Val  Val Pro Glu Phe Val  Asn Ala Ser 
    2120                 2125                 2130             


Ala Asp  Ala Gly Gly Ser Ser  Ala Thr Tyr Met Asp  Gln Ala Pro 
    2135                 2140                 2145             


Ser Pro  Ala Val Cys Pro Gln  Ala Pro Tyr Asn Met  Tyr Pro Gln 
    2150                 2155                 2160             


Asn Pro  Asp His Val Leu Asp  Gln Asp Gly Glu Phe  Asp Leu Asp 
    2165                 2170                 2175             


Glu Thr  Met Asp Val Ala Arg  His Val Glu Glu Leu  Leu Arg Arg 
    2180                 2185                 2190             


Pro Met  Asp Ser Leu Asp Ser  Arg Leu Ser Pro Pro  Ala Gly Leu 
    2195                 2200                 2205             


Phe Thr  Ser Ala Arg Gly Ser  Leu Ser Glu Gly Arg  Gly Ser Leu 
    2210                 2215                 2220             


Leu Thr  Cys Gly Asp Val Glu  Glu Asn Pro Gly Pro  Met Glu Thr 
    2225                 2230                 2235             


Asp Thr  Leu Leu Leu Trp Val  Leu Leu Leu Trp Val  Pro Gly Ser 
    2240                 2245                 2250             


Thr Gly  Asp Ile Gln Met Thr  Gln Thr Thr Ser Ser  Leu Ser Ala 
    2255                 2260                 2265             


Ser Leu  Gly Asp Arg Val Thr  Ile Ser Cys Arg Ala  Ser Gln Asp 
    2270                 2275                 2280             


Ile Ser  Lys Tyr Leu Asn Trp  Tyr Gln Gln Lys Pro  Asp Gly Thr 
    2285                 2290                 2295             


Val Lys  Leu Leu Ile Tyr His  Thr Ser Arg Leu His  Ser Gly Val 
    2300                 2305                 2310             


Pro Ser  Arg Phe Ser Gly Ser  Gly Ser Gly Thr Asp  Tyr Ser Leu 
    2315                 2320                 2325             


Thr Ile  Ser Asn Leu Glu Gln  Glu Asp Ile Ala Thr  Tyr Phe Cys 
    2330                 2335                 2340             


Gln Gln  Gly Asn Thr Leu Pro  Tyr Thr Phe Gly Gly  Gly Thr Lys 
    2345                 2350                 2355             


Leu Glu  Ile Thr Lys Ala Gly  Gly Gly Gly Ser Gly  Gly Gly Gly 
    2360                 2365                 2370             


Ser Gly  Gly Gly Gly Ser Gly  Gly Gly Gly Ser Glu  Val Lys Leu 
    2375                 2380                 2385             


Gln Glu  Ser Gly Pro Gly Leu  Val Ala Pro Ser Gln  Ser Leu Ser 
    2390                 2395                 2400             


Val Thr  Cys Thr Val Ser Gly  Val Ser Leu Pro Asp  Tyr Gly Val 
    2405                 2410                 2415             


Ser Trp  Ile Arg Gln Pro Pro  Arg Lys Gly Leu Glu  Trp Leu Gly 
    2420                 2425                 2430             


Val Ile  Trp Gly Ser Glu Thr  Thr Tyr Tyr Asn Ser  Ala Leu Lys 
    2435                 2440                 2445             


Ser Arg  Leu Thr Ile Ile Lys  Asp Asn Ser Lys Ser  Gln Val Phe 
    2450                 2455                 2460             


Leu Lys  Met Asn Ser Leu Gln  Thr Asp Asp Thr Ala  Ile Tyr Tyr 
    2465                 2470                 2475             


Cys Ala  Lys His Tyr Tyr Tyr  Gly Gly Ser Tyr Ala  Met Asp Tyr 
    2480                 2485                 2490             


Trp Gly  Gln Gly Thr Ser Val  Thr Val Ser Ser Asp  Pro Thr Thr 
    2495                 2500                 2505             


Thr Pro  Ala Pro Arg Pro Pro  Thr Pro Ala Pro Thr  Ile Ala Ser 
    2510                 2515                 2520             


Gln Pro  Leu Ser Leu Arg Pro  Glu Ala Cys Arg Pro  Ala Ala Gly 
    2525                 2530                 2535             


Gly Ala  Val His Thr Arg Gly  Leu Asp Phe Ala Cys  Asp Ile Tyr 
    2540                 2545                 2550             


Ile Trp  Ala Pro Leu Ala Gly  Thr Cys Gly Val Leu  Leu Leu Ser 
    2555                 2560                 2565             


Leu Val  Ile Thr Leu Tyr Cys  Lys Arg Gly Arg Lys  Lys Leu Leu 
    2570                 2575                 2580             


Tyr Ile  Phe Lys Gln Pro Phe  Met Arg Pro Val Gln  Thr Thr Gln 
    2585                 2590                 2595             


Glu Glu  Asp Gly Cys Ser Cys  Arg Phe Pro Glu Glu  Glu Glu Gly 
    2600                 2605                 2610             


Gly Cys  Glu Leu Arg Val Lys  Phe Ser Arg Ser Ala  Asp Ala Pro 
    2615                 2620                 2625             


Ala Tyr  Gln Gln Gly Gln Asn  Gln Leu Tyr Asn Glu  Leu Asn Leu 
    2630                 2635                 2640             


Gly Arg  Arg Glu Glu Tyr Asp  Val Leu Asp Lys Arg  Arg Gly Arg 
    2645                 2650                 2655             


Asp Pro  Glu Met Gly Gly Lys  Pro Arg Arg Lys Asn  Pro Gln Glu 
    2660                 2665                 2670             


Gly Leu  Tyr Asn Glu Leu Gln  Lys Asp Lys Met Ala  Glu Ala Tyr 
    2675                 2680                 2685             


Ser Glu  Ile Gly Met Lys Gly  Glu Arg Arg Arg Gly  Lys Gly His 
    2690                 2695                 2700             


Asp Gly  Leu Tyr Gln Gly Leu  Ser Thr Ala Thr Lys  Asp Thr Tyr 
    2705                 2710                 2715             


Asp Ala  Leu His Met Gln Ala  Leu Pro Pro Arg 
    2720                 2725                 


<210>  10
<211>  2430
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  constitutively active STAT construct (TIP - Linker - dZap70SH2 - 
       linker - TEV - 2a - Truncated CD22 - CD19TM - Tyro-1 endodomain -
       Linker - TetRB - 2A - aCD19 - linker - TEV cleavage site x 3 - 
       GOFSTAT5(S710F)

<400>  10

Met Trp Thr Trp Asn Ala Tyr Ala Phe Ala Ala Pro Ser Gly Gly Gly 
1               5                   10                  15      


Ser Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile 
            20                  25                  30          


Ser Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp 
        35                  40                  45              


Gly Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val 
    50                  55                  60                  


Leu Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg 
65                  70                  75                  80  


Gln Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly 
                85                  90                  95      


Pro Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro 
            100                 105                 110         


Cys Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln 
        115                 120                 125             


Pro Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val 
    130                 135                 140                 


Arg Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile 
145                 150                 155                 160 


Ser Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu 
                165                 170                 175     


Arg Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg 
            180                 185                 190         


Lys Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro 
        195                 200                 205             


Arg Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr 
    210                 215                 220                 


Val Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile 
225                 230                 235                 240 


Pro Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu 
                245                 250                 255     


Lys Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro 
            260                 265                 270         


Asn Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro 
        275                 280                 285             


Ala His Pro Ser Thr Leu Thr His Pro Ser Gly Gly Gly Gly Ser Ser 
    290                 295                 300                 


Leu Phe Lys Gly Pro Arg Asp Tyr Asn Pro Ile Ser Ser Thr Ile Cys 
305                 310                 315                 320 


His Leu Thr Asn Glu Ser Asp Gly His Thr Thr Ser Leu Tyr Gly Ile 
                325                 330                 335     


Gly Phe Gly Pro Phe Ile Ile Thr Asn Lys His Leu Phe Arg Arg Asn 
            340                 345                 350         


Asn Gly Thr Leu Leu Val Gln Ser Leu His Gly Val Phe Lys Val Lys 
        355                 360                 365             


Asn Thr Thr Thr Leu Gln Gln His Leu Ile Asp Gly Arg Asp Met Ile 
    370                 375                 380                 


Ile Ile Arg Met Pro Lys Asp Phe Pro Pro Phe Pro Gln Lys Leu Lys 
385                 390                 395                 400 


Phe Arg Glu Pro Gln Arg Glu Glu Arg Ile Cys Leu Val Thr Thr Asn 
                405                 410                 415     


Phe Gln Thr Lys Ser Met Ser Ser Met Val Ser Asp Thr Ser Cys Thr 
            420                 425                 430         


Phe Pro Ser Ser Asp Gly Ile Phe Trp Lys His Trp Ile Gln Thr Lys 
        435                 440                 445             


Asp Gly Gln Cys Gly Ser Pro Leu Val Ser Thr Arg Asp Gly Phe Ile 
    450                 455                 460                 


Val Gly Ile His Ser Ala Ser Asn Phe Thr Asn Thr Asn Asn Tyr Phe 
465                 470                 475                 480 


Thr Ser Val Pro Lys Asn Phe Met Glu Leu Leu Thr Asn Gln Glu Ala 
                485                 490                 495     


Gln Gln Trp Val Ser Gly Trp Arg Leu Asn Ala Asp Ser Val Leu Trp 
            500                 505                 510         


Gly Gly His Lys Val Phe Met Ser Lys Pro Glu Glu Pro Phe Gln Pro 
        515                 520                 525             


Val Lys Glu Ala Thr Gln Leu Met Asn Glu Leu Val Tyr Ser Gln Glu 
    530                 535                 540                 


Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly 
545                 550                 555                 560 


Pro Met Glu Thr Asp Thr Leu Leu Leu Trp Val Leu Leu Leu Trp Val 
                565                 570                 575     


Pro Gly Ser Thr Gly Asp Ser Ser Gly Lys Pro Ile Pro Asn Pro Leu 
            580                 585                 590         


Leu Gly Leu Asp Ser Ser Gly Gly Gly Ser Ala Pro Arg Asp Val Arg 
        595                 600                 605             


Val Arg Lys Ile Lys Pro Leu Ser Glu Ile His Ser Gly Asn Ser Val 
    610                 615                 620                 


Ser Leu Gln Cys Asp Phe Ser Ser Ser His Pro Lys Glu Val Gln Phe 
625                 630                 635                 640 


Phe Trp Glu Lys Asn Gly Arg Leu Leu Gly Lys Glu Ser Gln Leu Asn 
                645                 650                 655     


Phe Asp Ser Ile Ser Pro Glu Asp Ala Gly Ser Tyr Ser Cys Trp Val 
            660                 665                 670         


Asn Asn Ser Ile Gly Gln Thr Ala Ser Lys Ala Trp Thr Leu Glu Val 
        675                 680                 685             


Leu Tyr Ala Pro Arg Arg Leu Arg Val Ser Met Ser Pro Gly Asp Gln 
    690                 695                 700                 


Val Met Glu Gly Lys Ser Ala Thr Leu Thr Cys Glu Ser Asp Ala Asn 
705                 710                 715                 720 


Pro Pro Val Ser His Tyr Thr Trp Phe Asp Trp Asn Asn Gln Ser Leu 
                725                 730                 735     


Pro Tyr His Ser Gln Lys Leu Arg Leu Glu Pro Val Lys Val Gln His 
            740                 745                 750         


Ser Gly Ala Tyr Trp Cys Gln Gly Thr Asn Ser Val Gly Lys Gly Arg 
        755                 760                 765             


Ser Pro Leu Ser Thr Leu Thr Val Tyr Tyr Ser Pro Glu Thr Ile Gly 
    770                 775                 780                 


Arg Arg Ala Val Thr Leu Ala Tyr Leu Ile Phe Cys Leu Cys Ser Leu 
785                 790                 795                 800 


Val Gly Ile Leu His Leu Arg Ala Arg Arg Ser Met Asp Glu Ala Asn 
                805                 810                 815     


Gln Pro Leu Leu Thr Asp Gln Tyr Gln Cys Tyr Ala Glu Glu Tyr Glu 
            820                 825                 830         


Lys Leu Gln Asn Pro Asn Gln Ser Val Val Gly Gly Ser Gly Gly Ser 
        835                 840                 845             


Met Ser Arg Leu Asp Lys Ser Lys Val Ile Asn Ser Ala Leu Glu Leu 
    850                 855                 860                 


Leu Asn Glu Val Gly Ile Glu Gly Leu Thr Thr Arg Lys Leu Ala Gln 
865                 870                 875                 880 


Lys Leu Gly Val Glu Gln Pro Thr Leu Tyr Trp His Val Lys Asn Lys 
                885                 890                 895     


Arg Ala Leu Leu Asp Ala Leu Ala Ile Glu Met Leu Asp Arg His His 
            900                 905                 910         


Thr His Phe Cys Pro Leu Glu Gly Glu Ser Trp Gln Asp Phe Leu Arg 
        915                 920                 925             


Asn Asn Ala Lys Ser Phe Arg Cys Ala Leu Leu Ser His Arg Asp Gly 
    930                 935                 940                 


Ala Lys Val His Leu Gly Thr Arg Pro Thr Glu Lys Gln Tyr Glu Thr 
945                 950                 955                 960 


Leu Glu Asn Gln Leu Ala Phe Leu Cys Gln Gln Gly Phe Ser Leu Glu 
                965                 970                 975     


Asn Ala Leu Tyr Ala Leu Ser Ala Val Gly His Phe Thr Leu Gly Cys 
            980                 985                 990         


Val Leu Glu Asp Gln Glu His Gln  Val Ala Lys Glu Glu  Arg Glu Thr 
        995                 1000                 1005             


Pro Thr  Thr Asp Ser Met Pro  Pro Leu Leu Arg Gln  Ala Ile Glu 
    1010                 1015                 1020             


Leu Phe  Asp His Gln Gly Ala  Glu Pro Ala Phe Leu  Phe Gly Leu 
    1025                 1030                 1035             


Glu Leu  Ile Ile Cys Gly Leu  Glu Lys Gln Leu Lys  Cys Glu Ser 
    1040                 1045                 1050             


Gly Ser  Glu Gly Arg Gly Ser  Leu Leu Thr Cys Gly  Asp Val Glu 
    1055                 1060                 1065             


Glu Asn  Pro Gly Pro Met Glu  Thr Asp Thr Leu Leu  Leu Trp Val 
    1070                 1075                 1080             


Leu Leu  Leu Trp Val Pro Gly  Ser Thr Gly Asp Ile  Gln Met Thr 
    1085                 1090                 1095             


Gln Thr  Thr Ser Ser Leu Ser  Ala Ser Leu Gly Asp  Arg Val Thr 
    1100                 1105                 1110             


Ile Ser  Cys Arg Ala Ser Gln  Asp Ile Ser Lys Tyr  Leu Asn Trp 
    1115                 1120                 1125             


Tyr Gln  Gln Lys Pro Asp Gly  Thr Val Lys Leu Leu  Ile Tyr His 
    1130                 1135                 1140             


Thr Ser  Arg Leu His Ser Gly  Val Pro Ser Arg Phe  Ser Gly Ser 
    1145                 1150                 1155             


Gly Ser  Gly Thr Asp Tyr Ser  Leu Thr Ile Ser Asn  Leu Glu Gln 
    1160                 1165                 1170             


Glu Asp  Ile Ala Thr Tyr Phe  Cys Gln Gln Gly Asn  Thr Leu Pro 
    1175                 1180                 1185             


Tyr Thr  Phe Gly Gly Gly Thr  Lys Leu Glu Ile Thr  Lys Ala Gly 
    1190                 1195                 1200             


Gly Gly  Gly Ser Gly Gly Gly  Gly Ser Gly Gly Gly  Gly Ser Gly 
    1205                 1210                 1215             


Gly Gly  Gly Ser Glu Val Lys  Leu Gln Glu Ser Gly  Pro Gly Leu 
    1220                 1225                 1230             


Val Ala  Pro Ser Gln Ser Leu  Ser Val Thr Cys Thr  Val Ser Gly 
    1235                 1240                 1245             


Val Ser  Leu Pro Asp Tyr Gly  Val Ser Trp Ile Arg  Gln Pro Pro 
    1250                 1255                 1260             


Arg Lys  Gly Leu Glu Trp Leu  Gly Val Ile Trp Gly  Ser Glu Thr 
    1265                 1270                 1275             


Thr Tyr  Tyr Asn Ser Ala Leu  Lys Ser Arg Leu Thr  Ile Ile Lys 
    1280                 1285                 1290             


Asp Asn  Ser Lys Ser Gln Val  Phe Leu Lys Met Asn  Ser Leu Gln 
    1295                 1300                 1305             


Thr Asp  Asp Thr Ala Ile Tyr  Tyr Cys Ala Lys His  Tyr Tyr Tyr 
    1310                 1315                 1320             


Gly Gly  Ser Tyr Ala Met Asp  Tyr Trp Gly Gln Gly  Thr Ser Val 
    1325                 1330                 1335             


Thr Val  Ser Ser Asp Pro Thr  Thr Thr Pro Ala Pro  Arg Pro Pro 
    1340                 1345                 1350             


Thr Pro  Ala Pro Thr Ile Ala  Ser Gln Pro Leu Ser  Leu Arg Pro 
    1355                 1360                 1365             


Glu Ala  Cys Arg Pro Ala Ala  Gly Gly Ala Val His  Thr Arg Gly 
    1370                 1375                 1380             


Leu Asp  Phe Ala Cys Asp Ile  Tyr Ile Trp Ala Pro  Leu Ala Gly 
    1385                 1390                 1395             


Thr Cys  Gly Val Leu Leu Leu  Ser Leu Val Ile Thr  Leu Tyr Cys 
    1400                 1405                 1410             


Lys Arg  Gly Arg Lys Lys Leu  Leu Tyr Ile Phe Lys  Gln Pro Phe 
    1415                 1420                 1425             


Met Arg  Pro Val Gln Thr Thr  Gln Glu Glu Asp Gly  Cys Ser Cys 
    1430                 1435                 1440             


Arg Phe  Pro Glu Glu Glu Glu  Gly Gly Cys Glu Leu  Arg Val Lys 
    1445                 1450                 1455             


Phe Ser  Arg Ser Ala Asp Ala  Pro Ala Tyr Gln Gln  Gly Gln Asn 
    1460                 1465                 1470             


Gln Leu  Tyr Asn Glu Leu Asn  Leu Gly Arg Arg Glu  Glu Tyr Asp 
    1475                 1480                 1485             


Val Leu  Asp Lys Arg Arg Gly  Arg Asp Pro Glu Met  Gly Gly Lys 
    1490                 1495                 1500             


Pro Arg  Arg Lys Asn Pro Gln  Glu Gly Leu Tyr Asn  Glu Leu Gln 
    1505                 1510                 1515             


Lys Asp  Lys Met Ala Glu Ala  Tyr Ser Glu Ile Gly  Met Lys Gly 
    1520                 1525                 1530             


Glu Arg  Arg Arg Gly Lys Gly  His Asp Gly Leu Tyr  Gln Gly Leu 
    1535                 1540                 1545             


Ser Thr  Ala Thr Lys Asp Thr  Tyr Asp Ala Leu His  Met Gln Ala 
    1550                 1555                 1560             


Leu Pro  Pro Arg Gly Gly Ser  Gly Gly Ser Glu Asn  Leu Tyr Phe 
    1565                 1570                 1575             


Gln Gly  Glu Asn Leu Tyr Phe  Gln Gly Glu Asn Leu  Tyr Phe Gln 
    1580                 1585                 1590             


Gly Glu  Asn Leu Tyr Phe Gln  Gly Glu Asn Leu Tyr  Phe Gln Gly 
    1595                 1600                 1605             


Glu Asn  Leu Tyr Phe Gln Gly  Glu Asn Leu Tyr Phe  Gln Gly Glu 
    1610                 1615                 1620             


Asn Leu  Tyr Phe Gln Gly Glu  Asn Leu Tyr Phe Gln  Gly Met Ala 
    1625                 1630                 1635             


Gly Trp  Ile Gln Ala Gln Gln  Leu Gln Gly Asp Ala  Leu Arg Gln 
    1640                 1645                 1650             


Met Gln  Val Leu Tyr Gly Gln  His Phe Pro Ile Glu  Val Arg His 
    1655                 1660                 1665             


Tyr Leu  Ala Gln Trp Ile Glu  Ser Gln Pro Trp Asp  Ala Ile Asp 
    1670                 1675                 1680             


Leu Asp  Asn Pro Gln Asp Arg  Ala Gln Ala Thr Gln  Leu Leu Glu 
    1685                 1690                 1695             


Gly Leu  Val Gln Glu Leu Gln  Lys Lys Ala Glu His  Gln Val Gly 
    1700                 1705                 1710             


Glu Asp  Gly Phe Leu Leu Lys  Ile Lys Leu Gly His  Tyr Ala Thr 
    1715                 1720                 1725             


Gln Leu  Gln Lys Thr Tyr Asp  Arg Cys Pro Leu Glu  Leu Val Arg 
    1730                 1735                 1740             


Cys Ile  Arg His Ile Leu Tyr  Asn Glu Gln Arg Leu  Val Arg Glu 
    1745                 1750                 1755             


Ala Asn  Asn Cys Ser Ser Pro  Ala Gly Ile Leu Val  Asp Ala Met 
    1760                 1765                 1770             


Ser Gln  Lys His Leu Gln Ile  Asn Gln Thr Phe Glu  Glu Leu Arg 
    1775                 1780                 1785             


Leu Val  Thr Gln Asp Thr Glu  Asn Glu Leu Lys Lys  Leu Gln Gln 
    1790                 1795                 1800             


Thr Gln  Glu Tyr Phe Ile Ile  Gln Tyr Gln Glu Ser  Leu Arg Ile 
    1805                 1810                 1815             


Gln Ala  Gln Phe Ala Gln Leu  Ala Gln Leu Ser Pro  Gln Glu Arg 
    1820                 1825                 1830             


Leu Ser  Arg Glu Thr Ala Leu  Gln Gln Lys Gln Val  Ser Leu Glu 
    1835                 1840                 1845             


Ala Trp  Leu Gln Arg Glu Ala  Gln Thr Leu Gln Gln  Tyr Arg Val 
    1850                 1855                 1860             


Glu Leu  Ala Glu Lys His Gln  Lys Thr Leu Gln Leu  Leu Arg Lys 
    1865                 1870                 1875             


Gln Gln  Thr Ile Ile Leu Asp  Asp Glu Leu Ile Gln  Trp Lys Arg 
    1880                 1885                 1890             


Arg Gln  Gln Leu Ala Gly Asn  Gly Gly Pro Pro Glu  Gly Ser Leu 
    1895                 1900                 1905             


Asp Val  Leu Gln Ser Trp Cys  Glu Lys Leu Ala Glu  Ile Ile Trp 
    1910                 1915                 1920             


Gln Asn  Arg Gln Gln Ile Arg  Arg Ala Glu His Leu  Cys Gln Gln 
    1925                 1930                 1935             


Leu Pro  Ile Pro Gly Pro Val  Glu Glu Met Leu Ala  Glu Val Asn 
    1940                 1945                 1950             


Ala Thr  Ile Thr Asp Ile Ile  Ser Ala Leu Val Thr  Ser Thr Phe 
    1955                 1960                 1965             


Ile Ile  Glu Lys Gln Pro Pro  Gln Val Leu Lys Thr  Gln Thr Lys 
    1970                 1975                 1980             


Phe Ala  Ala Thr Val Arg Leu  Leu Val Gly Gly Lys  Leu Asn Val 
    1985                 1990                 1995             


His Met  Asn Pro Pro Gln Val  Lys Ala Thr Ile Ile  Ser Glu Gln 
    2000                 2005                 2010             


Gln Ala  Lys Ser Leu Leu Lys  Asn Glu Asn Thr Arg  Asn Glu Cys 
    2015                 2020                 2025             


Ser Gly  Glu Ile Leu Asn Asn  Cys Cys Val Met Glu  Tyr His Gln 
    2030                 2035                 2040             


Ala Thr  Gly Thr Leu Ser Ala  His Phe Arg Asn Met  Ser Leu Lys 
    2045                 2050                 2055             


Arg Ile  Lys Arg Ala Asp Arg  Arg Gly Ala Glu Ser  Val Thr Glu 
    2060                 2065                 2070             


Glu Lys  Phe Thr Val Leu Phe  Glu Ser Gln Phe Ser  Val Gly Ser 
    2075                 2080                 2085             


Asn Glu  Leu Val Phe Gln Val  Lys Thr Leu Ser Leu  Pro Val Val 
    2090                 2095                 2100             


Val Ile  Val His Gly Ser Gln  Asp His Asn Ala Thr  Ala Thr Val 
    2105                 2110                 2115             


Leu Trp  Asp Asn Ala Phe Ala  Glu Pro Gly Arg Val  Pro Phe Ala 
    2120                 2125                 2130             


Val Pro  Asp Lys Val Leu Trp  Pro Gln Leu Cys Glu  Ala Leu Asn 
    2135                 2140                 2145             


Met Lys  Phe Lys Ala Glu Val  Gln Ser Asn Arg Gly  Leu Thr Lys 
    2150                 2155                 2160             


Glu Asn  Leu Val Phe Leu Ala  Gln Lys Leu Phe Asn  Asn Ser Ser 
    2165                 2170                 2175             


Ser His  Leu Glu Asp Tyr Ser  Gly Leu Ser Val Ser  Trp Ser Gln 
    2180                 2185                 2190             


Phe Asn  Arg Glu Asn Leu Pro  Gly Trp Asn Tyr Thr  Phe Trp Gln 
    2195                 2200                 2205             


Trp Phe  Asp Gly Val Met Glu  Val Leu Lys Lys His  His Lys Pro 
    2210                 2215                 2220             


His Trp  Asn Asp Gly Ala Ile  Leu Gly Phe Val Asn  Lys Gln Gln 
    2225                 2230                 2235             


Ala His  Asp Leu Leu Ile Asn  Lys Pro Asp Gly Thr  Phe Leu Leu 
    2240                 2245                 2250             


Arg Phe  Ser Asp Ser Glu Ile  Gly Gly Ile Thr Ile  Ala Trp Lys 
    2255                 2260                 2265             


Phe Asp  Ser Pro Glu Arg Asn  Leu Trp Asn Leu Lys  Pro Phe Thr 
    2270                 2275                 2280             


Thr Arg  Asp Phe Ser Ile Arg  Ser Leu Ala Asp Arg  Leu Gly Asp 
    2285                 2290                 2295             


Leu Ser  Tyr Leu Ile Tyr Val  Phe Pro Asp Arg Pro  Lys Asp Glu 
    2300                 2305                 2310             


Val Phe  Ser Lys Tyr Tyr Thr  Pro Val Leu Ala Lys  Ala Val Asp 
    2315                 2320                 2325             


Gly Tyr  Val Lys Pro Gln Ile  Lys Gln Val Val Pro  Glu Phe Val 
    2330                 2335                 2340             


Asn Ala  Phe Ala Asp Ala Gly  Gly Ser Ser Ala Thr  Tyr Met Asp 
    2345                 2350                 2355             


Gln Ala  Pro Ser Pro Ala Val  Cys Pro Gln Ala Pro  Tyr Asn Met 
    2360                 2365                 2370             


Tyr Pro  Gln Asn Pro Asp His  Val Leu Asp Gln Asp  Gly Glu Phe 
    2375                 2380                 2385             


Asp Leu  Asp Glu Thr Met Asp  Val Ala Arg His Val  Glu Glu Leu 
    2390                 2395                 2400             


Leu Arg  Arg Pro Met Asp Ser  Leu Asp Ser Arg Leu  Ser Pro Pro 
    2405                 2410                 2415             


Ala Gly  Leu Phe Thr Ser Ala  Arg Gly Ser Leu Ser  
    2420                 2425                 2430 


<210>  11
<211>  1813
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  constitutively active STAT construct (FRB - linker - STAT5 - 2a -
       FKBP12 - linker - STAT3)

<400>  11

Ile Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu Ala Ser Arg 
1               5                   10                  15      


Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu Val Leu Glu 
            20                  25                  30          


Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu Lys Glu Thr 
        35                  40                  45              


Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala Gln Glu Trp 
    50                  55                  60                  


Cys Arg Lys Tyr Met Lys Ser Gly Asn Val Lys Asp Leu Leu Gln Ala 
65                  70                  75                  80  


Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Lys Ser Gly Gly 
                85                  90                  95      


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Ala Gly 
            100                 105                 110         


Trp Ile Gln Ala Gln Gln Leu Gln Gly Asp Ala Leu Arg Gln Met Gln 
        115                 120                 125             


Val Leu Tyr Gly Gln His Phe Pro Ile Glu Val Arg His Tyr Leu Ala 
    130                 135                 140                 


Gln Trp Ile Glu Ser Gln Pro Trp Asp Ala Ile Asp Leu Asp Asn Pro 
145                 150                 155                 160 


Gln Asp Arg Ala Gln Ala Thr Gln Leu Leu Glu Gly Leu Val Gln Glu 
                165                 170                 175     


Leu Gln Lys Lys Ala Glu His Gln Val Gly Glu Asp Gly Phe Leu Leu 
            180                 185                 190         


Lys Ile Lys Leu Gly His Tyr Ala Thr Gln Leu Gln Lys Thr Tyr Asp 
        195                 200                 205             


Arg Cys Pro Leu Glu Leu Val Arg Cys Ile Arg His Ile Leu Tyr Asn 
    210                 215                 220                 


Glu Gln Arg Leu Val Arg Glu Ala Asn Asn Cys Ser Ser Pro Ala Gly 
225                 230                 235                 240 


Ile Leu Val Asp Ala Met Ser Gln Lys His Leu Gln Ile Asn Gln Thr 
                245                 250                 255     


Phe Glu Glu Leu Arg Leu Val Thr Gln Asp Thr Glu Asn Glu Leu Lys 
            260                 265                 270         


Lys Leu Gln Gln Thr Gln Glu Tyr Phe Ile Ile Gln Tyr Gln Glu Ser 
        275                 280                 285             


Leu Arg Ile Gln Ala Gln Phe Ala Gln Leu Ala Gln Leu Ser Pro Gln 
    290                 295                 300                 


Glu Arg Leu Ser Arg Glu Thr Ala Leu Gln Gln Lys Gln Val Ser Leu 
305                 310                 315                 320 


Glu Ala Trp Leu Gln Arg Glu Ala Gln Thr Leu Gln Gln Tyr Arg Val 
                325                 330                 335     


Glu Leu Ala Glu Lys His Gln Lys Thr Leu Gln Leu Leu Arg Lys Gln 
            340                 345                 350         


Gln Thr Ile Ile Leu Asp Asp Glu Leu Ile Gln Trp Lys Arg Arg Gln 
        355                 360                 365             


Gln Leu Ala Gly Asn Gly Gly Pro Pro Glu Gly Ser Leu Asp Val Leu 
    370                 375                 380                 


Gln Ser Trp Cys Glu Lys Leu Ala Glu Ile Ile Trp Gln Asn Arg Gln 
385                 390                 395                 400 


Gln Ile Arg Arg Ala Glu His Leu Cys Gln Gln Leu Pro Ile Pro Gly 
                405                 410                 415     


Pro Val Glu Glu Met Leu Ala Glu Val Asn Ala Thr Ile Thr Asp Ile 
            420                 425                 430         


Ile Ser Ala Leu Val Thr Ser Thr Phe Ile Ile Glu Lys Gln Pro Pro 
        435                 440                 445             


Gln Val Leu Lys Thr Gln Thr Lys Phe Ala Ala Thr Val Arg Leu Leu 
    450                 455                 460                 


Val Gly Gly Lys Leu Asn Val His Met Asn Pro Pro Gln Val Lys Ala 
465                 470                 475                 480 


Thr Ile Ile Ser Glu Gln Gln Ala Lys Ser Leu Leu Lys Asn Glu Asn 
                485                 490                 495     


Thr Arg Asn Glu Cys Ser Gly Glu Ile Leu Asn Asn Cys Cys Val Met 
            500                 505                 510         


Glu Tyr His Gln Ala Thr Gly Thr Leu Ser Ala His Phe Arg Asn Met 
        515                 520                 525             


Ser Leu Lys Arg Ile Lys Arg Ala Asp Arg Arg Gly Ala Glu Ser Val 
    530                 535                 540                 


Thr Glu Glu Lys Phe Thr Val Leu Phe Glu Ser Gln Phe Ser Val Gly 
545                 550                 555                 560 


Ser Asn Glu Leu Val Phe Gln Val Lys Thr Leu Ser Leu Pro Val Val 
                565                 570                 575     


Val Ile Val His Gly Ser Gln Asp His Asn Ala Thr Ala Thr Val Leu 
            580                 585                 590         


Trp Asp Asn Ala Phe Ala Glu Pro Gly Arg Val Pro Phe Ala Val Pro 
        595                 600                 605             


Asp Lys Val Leu Trp Pro Gln Leu Cys Glu Ala Leu Asn Met Lys Phe 
    610                 615                 620                 


Lys Ala Glu Val Gln Ser Asn Arg Gly Leu Thr Lys Glu Asn Leu Val 
625                 630                 635                 640 


Phe Leu Ala Gln Lys Leu Phe Asn Asn Ser Ser Ser His Leu Glu Asp 
                645                 650                 655     


Tyr Ser Gly Leu Ser Val Ser Trp Ser Gln Phe Asn Arg Glu Asn Leu 
            660                 665                 670         


Pro Gly Trp Asn Tyr Thr Phe Trp Gln Trp Phe Asp Gly Val Met Glu 
        675                 680                 685             


Val Leu Lys Lys His His Lys Pro His Trp Asn Asp Gly Ala Ile Leu 
    690                 695                 700                 


Gly Phe Val Asn Lys Gln Gln Ala His Asp Leu Leu Ile Asn Lys Pro 
705                 710                 715                 720 


Asp Gly Thr Phe Leu Leu Arg Phe Ser Asp Ser Glu Ile Gly Gly Ile 
                725                 730                 735     


Thr Ile Ala Trp Lys Phe Asp Ser Pro Glu Arg Asn Leu Trp Asn Leu 
            740                 745                 750         


Lys Pro Phe Thr Thr Arg Asp Phe Ser Ile Arg Ser Leu Ala Asp Arg 
        755                 760                 765             


Leu Gly Asp Leu Ser Tyr Leu Ile Tyr Val Phe Pro Asp Arg Pro Lys 
    770                 775                 780                 


Asp Glu Val Phe Ser Lys Tyr Tyr Thr Pro Val Leu Ala Lys Ala Val 
785                 790                 795                 800 


Asp Gly Tyr Val Lys Pro Gln Ile Lys Gln Val Val Pro Glu Phe Val 
                805                 810                 815     


Asn Ala Ser Ala Asp Ala Gly Gly Ser Ser Ala Thr Tyr Met Asp Gln 
            820                 825                 830         


Ala Pro Ser Pro Ala Val Cys Pro Gln Ala Pro Tyr Asn Met Tyr Pro 
        835                 840                 845             


Gln Asn Pro Asp His Val Leu Asp Gln Asp Gly Glu Phe Asp Leu Asp 
    850                 855                 860                 


Glu Thr Met Asp Val Ala Arg His Val Glu Glu Leu Leu Arg Arg Pro 
865                 870                 875                 880 


Met Asp Ser Leu Asp Ser Arg Leu Ser Pro Pro Ala Gly Leu Phe Thr 
                885                 890                 895     


Ser Ala Arg Gly Ser Leu Ser Glu Gly Arg Gly Ser Leu Leu Thr Cys 
            900                 905                 910         


Gly Asp Val Glu Glu Asn Pro Gly Pro Gly Val Gln Val Glu Thr Ile 
        915                 920                 925             


Ser Pro Gly Asp Gly Arg Thr Phe Pro Lys Arg Gly Gln Thr Cys Val 
    930                 935                 940                 


Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Val Asp Ser Ser 
945                 950                 955                 960 


Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln Glu Val 
                965                 970                 975     


Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly Gln Arg 
            980                 985                 990         


Ala Lys Leu Thr Ile Ser Pro Asp  Tyr Ala Tyr Gly Ala  Thr Gly His 
        995                 1000                 1005             


Pro Gly  Ile Ile Pro Pro His  Ala Thr Leu Val Phe  Asp Val Glu 
    1010                 1015                 1020             


Leu Leu  Lys Leu Glu Ser Gly  Gly Gly Gly Ser Gly  Gly Gly Gly 
    1025                 1030                 1035             


Ser Gly  Gly Gly Gly Ser Ala  Gln Trp Asn Gln Leu  Gln Gln Leu 
    1040                 1045                 1050             


Asp Thr  Arg Tyr Leu Glu Gln  Leu His Gln Leu Tyr  Ser Asp Ser 
    1055                 1060                 1065             


Phe Pro  Met Glu Leu Arg Gln  Phe Leu Ala Pro Trp  Ile Glu Ser 
    1070                 1075                 1080             


Gln Asp  Trp Ala Tyr Ala Ala  Ser Lys Glu Ser His  Ala Thr Leu 
    1085                 1090                 1095             


Val Phe  His Asn Leu Leu Gly  Glu Ile Asp Gln Gln  Tyr Ser Arg 
    1100                 1105                 1110             


Phe Leu  Gln Glu Ser Asn Val  Leu Tyr Gln His Asn  Leu Arg Arg 
    1115                 1120                 1125             


Ile Lys  Gln Phe Leu Gln Ser  Arg Tyr Leu Glu Lys  Pro Met Glu 
    1130                 1135                 1140             


Ile Ala  Arg Ile Val Ala Arg  Cys Leu Trp Glu Glu  Ser Arg Leu 
    1145                 1150                 1155             


Leu Gln  Thr Ala Ala Thr Ala  Ala Gln Gln Gly Gly  Gln Ala Asn 
    1160                 1165                 1170             


His Pro  Thr Ala Ala Val Val  Thr Glu Lys Gln Gln  Met Leu Glu 
    1175                 1180                 1185             


Gln His  Leu Gln Asp Val Arg  Lys Arg Val Gln Asp  Leu Glu Gln 
    1190                 1195                 1200             


Lys Met  Lys Val Val Glu Asn  Leu Gln Asp Asp Phe  Asp Phe Asn 
    1205                 1210                 1215             


Tyr Lys  Thr Leu Lys Ser Gln  Gly Asp Met Gln Asp  Leu Asn Gly 
    1220                 1225                 1230             


Asn Asn  Gln Ser Val Thr Arg  Gln Lys Met Gln Gln  Leu Glu Gln 
    1235                 1240                 1245             


Met Leu  Thr Ala Leu Asp Gln  Met Arg Arg Ser Ile  Val Ser Glu 
    1250                 1255                 1260             


Leu Ala  Gly Leu Leu Ser Ala  Met Glu Tyr Val Gln  Lys Thr Leu 
    1265                 1270                 1275             


Thr Asp  Glu Glu Leu Ala Asp  Trp Lys Arg Arg Gln  Gln Ile Ala 
    1280                 1285                 1290             


Cys Ile  Gly Gly Pro Pro Asn  Ile Cys Leu Asp Arg  Leu Glu Asn 
    1295                 1300                 1305             


Trp Ile  Thr Ser Leu Ala Glu  Ser Gln Leu Gln Thr  Arg Gln Gln 
    1310                 1315                 1320             


Ile Lys  Lys Leu Glu Glu Leu  Gln Gln Lys Val Ser  Tyr Lys Gly 
    1325                 1330                 1335             


Asp Pro  Ile Val Gln His Arg  Pro Met Leu Glu Glu  Arg Ile Val 
    1340                 1345                 1350             


Glu Leu  Phe Arg Asn Leu Met  Lys Ser Ala Phe Val  Val Glu Arg 
    1355                 1360                 1365             


Gln Pro  Cys Met Pro Met His  Pro Asp Arg Pro Leu  Val Ile Lys 
    1370                 1375                 1380             


Thr Gly  Val Gln Phe Thr Thr  Lys Val Arg Leu Leu  Val Lys Phe 
    1385                 1390                 1395             


Pro Glu  Leu Asn Tyr Gln Leu  Lys Ile Lys Val Cys  Ile Asp Lys 
    1400                 1405                 1410             


Asp Ser  Gly Asp Val Ala Ala  Leu Arg Gly Ser Arg  Lys Phe Asn 
    1415                 1420                 1425             


Ile Leu  Gly Thr Asn Thr Lys  Val Met Asn Met Glu  Glu Ser Asn 
    1430                 1435                 1440             


Asn Gly  Ser Leu Ser Ala Glu  Phe Lys His Leu Thr  Leu Arg Glu 
    1445                 1450                 1455             


Gln Arg  Cys Gly Asn Gly Gly  Arg Ala Asn Cys Asp  Ala Ser Leu 
    1460                 1465                 1470             


Ile Val  Thr Glu Glu Leu His  Leu Ile Thr Phe Glu  Thr Glu Val 
    1475                 1480                 1485             


Tyr His  Gln Gly Leu Lys Ile  Asp Leu Glu Thr His  Ser Leu Pro 
    1490                 1495                 1500             


Val Val  Val Ile Ser Asn Ile  Cys Gln Met Pro Asn  Ala Trp Ala 
    1505                 1510                 1515             


Ser Ile  Leu Trp Tyr Asn Met  Leu Thr Asn Asn Pro  Lys Asn Val 
    1520                 1525                 1530             


Asn Phe  Phe Thr Lys Pro Pro  Ile Gly Thr Trp Asp  Gln Val Ala 
    1535                 1540                 1545             


Glu Val  Leu Ser Trp Gln Phe  Ser Ser Thr Thr Lys  Arg Gly Leu 
    1550                 1555                 1560             


Ser Ile  Glu Gln Leu Thr Thr  Leu Ala Glu Lys Leu  Leu Gly Pro 
    1565                 1570                 1575             


Gly Val  Asn Tyr Ser Gly Cys  Gln Ile Thr Trp Ala  Lys Phe Cys 
    1580                 1585                 1590             


Lys Glu  Asn Met Ala Gly Lys  Gly Phe Ser Phe Trp  Val Trp Leu 
    1595                 1600                 1605             


Asp Asn  Ile Ile Asp Leu Val  Lys Lys Tyr Ile Leu  Ala Leu Trp 
    1610                 1615                 1620             


Asn Glu  Gly Tyr Ile Met Gly  Phe Ile Ser Lys Glu  Arg Glu Arg 
    1625                 1630                 1635             


Ala Ile  Leu Ser Thr Lys Pro  Pro Gly Thr Phe Leu  Leu Arg Phe 
    1640                 1645                 1650             


Ser Glu  Ser Ser Lys Glu Gly  Gly Val Thr Phe Thr  Trp Val Glu 
    1655                 1660                 1665             


Lys Asp  Ile Ser Gly Lys Thr  Gln Ile Gln Ser Val  Glu Pro Tyr 
    1670                 1675                 1680             


Thr Lys  Gln Gln Leu Asn Asn  Met Ser Phe Ala Glu  Ile Ile Met 
    1685                 1690                 1695             


Gly Tyr  Lys Ile Met Asp Ala  Thr Asn Ile Leu Val  Ser Pro Leu 
    1700                 1705                 1710             


Val Tyr  Leu Tyr Pro Asp Ile  Pro Lys Glu Glu Ala  Phe Gly Lys 
    1715                 1720                 1725             


Tyr Cys  Arg Pro Glu Ser Gln  Glu His Pro Glu Ala  Asp Pro Gly 
    1730                 1735                 1740             


Ser Ala  Ala Pro Tyr Leu Lys  Thr Lys Phe Ile Cys  Val Thr Pro 
    1745                 1750                 1755             


Thr Thr  Cys Ser Asn Thr Ile  Asp Leu Pro Met Ser  Pro Arg Thr 
    1760                 1765                 1770             


Leu Asp  Ser Leu Met Gln Phe  Gly Asn Asn Gly Glu  Gly Ala Glu 
    1775                 1780                 1785             


Pro Ser  Ala Gly Gly Gln Phe  Glu Ser Leu Thr Phe  Asp Met Glu 
    1790                 1795                 1800             


Leu Thr  Ser Glu Cys Ala Thr  Ser Pro Met 
    1805                 1810             


<210>  12
<211>  1832
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  constitutively active STAT construct (TIP - linker - STAT5 - 2a -
       TetRB - linker - STAT3)

<400>  12

Met Trp Thr Trp Asn Ala Tyr Ala Phe Ala Ala Pro Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Met Ala Gly Trp 
            20                  25                  30          


Ile Gln Ala Gln Gln Leu Gln Gly Asp Ala Leu Arg Gln Met Gln Val 
        35                  40                  45              


Leu Tyr Gly Gln His Phe Pro Ile Glu Val Arg His Tyr Leu Ala Gln 
    50                  55                  60                  


Trp Ile Glu Ser Gln Pro Trp Asp Ala Ile Asp Leu Asp Asn Pro Gln 
65                  70                  75                  80  


Asp Arg Ala Gln Ala Thr Gln Leu Leu Glu Gly Leu Val Gln Glu Leu 
                85                  90                  95      


Gln Lys Lys Ala Glu His Gln Val Gly Glu Asp Gly Phe Leu Leu Lys 
            100                 105                 110         


Ile Lys Leu Gly His Tyr Ala Thr Gln Leu Gln Lys Thr Tyr Asp Arg 
        115                 120                 125             


Cys Pro Leu Glu Leu Val Arg Cys Ile Arg His Ile Leu Tyr Asn Glu 
    130                 135                 140                 


Gln Arg Leu Val Arg Glu Ala Asn Asn Cys Ser Ser Pro Ala Gly Ile 
145                 150                 155                 160 


Leu Val Asp Ala Met Ser Gln Lys His Leu Gln Ile Asn Gln Thr Phe 
                165                 170                 175     


Glu Glu Leu Arg Leu Val Thr Gln Asp Thr Glu Asn Glu Leu Lys Lys 
            180                 185                 190         


Leu Gln Gln Thr Gln Glu Tyr Phe Ile Ile Gln Tyr Gln Glu Ser Leu 
        195                 200                 205             


Arg Ile Gln Ala Gln Phe Ala Gln Leu Ala Gln Leu Ser Pro Gln Glu 
    210                 215                 220                 


Arg Leu Ser Arg Glu Thr Ala Leu Gln Gln Lys Gln Val Ser Leu Glu 
225                 230                 235                 240 


Ala Trp Leu Gln Arg Glu Ala Gln Thr Leu Gln Gln Tyr Arg Val Glu 
                245                 250                 255     


Leu Ala Glu Lys His Gln Lys Thr Leu Gln Leu Leu Arg Lys Gln Gln 
            260                 265                 270         


Thr Ile Ile Leu Asp Asp Glu Leu Ile Gln Trp Lys Arg Arg Gln Gln 
        275                 280                 285             


Leu Ala Gly Asn Gly Gly Pro Pro Glu Gly Ser Leu Asp Val Leu Gln 
    290                 295                 300                 


Ser Trp Cys Glu Lys Leu Ala Glu Ile Ile Trp Gln Asn Arg Gln Gln 
305                 310                 315                 320 


Ile Arg Arg Ala Glu His Leu Cys Gln Gln Leu Pro Ile Pro Gly Pro 
                325                 330                 335     


Val Glu Glu Met Leu Ala Glu Val Asn Ala Thr Ile Thr Asp Ile Ile 
            340                 345                 350         


Ser Ala Leu Val Thr Ser Thr Phe Ile Ile Glu Lys Gln Pro Pro Gln 
        355                 360                 365             


Val Leu Lys Thr Gln Thr Lys Phe Ala Ala Thr Val Arg Leu Leu Val 
    370                 375                 380                 


Gly Gly Lys Leu Asn Val His Met Asn Pro Pro Gln Val Lys Ala Thr 
385                 390                 395                 400 


Ile Ile Ser Glu Gln Gln Ala Lys Ser Leu Leu Lys Asn Glu Asn Thr 
                405                 410                 415     


Arg Asn Glu Cys Ser Gly Glu Ile Leu Asn Asn Cys Cys Val Met Glu 
            420                 425                 430         


Tyr His Gln Ala Thr Gly Thr Leu Ser Ala His Phe Arg Asn Met Ser 
        435                 440                 445             


Leu Lys Arg Ile Lys Arg Ala Asp Arg Arg Gly Ala Glu Ser Val Thr 
    450                 455                 460                 


Glu Glu Lys Phe Thr Val Leu Phe Glu Ser Gln Phe Ser Val Gly Ser 
465                 470                 475                 480 


Asn Glu Leu Val Phe Gln Val Lys Thr Leu Ser Leu Pro Val Val Val 
                485                 490                 495     


Ile Val His Gly Ser Gln Asp His Asn Ala Thr Ala Thr Val Leu Trp 
            500                 505                 510         


Asp Asn Ala Phe Ala Glu Pro Gly Arg Val Pro Phe Ala Val Pro Asp 
        515                 520                 525             


Lys Val Leu Trp Pro Gln Leu Cys Glu Ala Leu Asn Met Lys Phe Lys 
    530                 535                 540                 


Ala Glu Val Gln Ser Asn Arg Gly Leu Thr Lys Glu Asn Leu Val Phe 
545                 550                 555                 560 


Leu Ala Gln Lys Leu Phe Asn Asn Ser Ser Ser His Leu Glu Asp Tyr 
                565                 570                 575     


Ser Gly Leu Ser Val Ser Trp Ser Gln Phe Asn Arg Glu Asn Leu Pro 
            580                 585                 590         


Gly Trp Asn Tyr Thr Phe Trp Gln Trp Phe Asp Gly Val Met Glu Val 
        595                 600                 605             


Leu Lys Lys His His Lys Pro His Trp Asn Asp Gly Ala Ile Leu Gly 
    610                 615                 620                 


Phe Val Asn Lys Gln Gln Ala His Asp Leu Leu Ile Asn Lys Pro Asp 
625                 630                 635                 640 


Gly Thr Phe Leu Leu Arg Phe Ser Asp Ser Glu Ile Gly Gly Ile Thr 
                645                 650                 655     


Ile Ala Trp Lys Phe Asp Ser Pro Glu Arg Asn Leu Trp Asn Leu Lys 
            660                 665                 670         


Pro Phe Thr Thr Arg Asp Phe Ser Ile Arg Ser Leu Ala Asp Arg Leu 
        675                 680                 685             


Gly Asp Leu Ser Tyr Leu Ile Tyr Val Phe Pro Asp Arg Pro Lys Asp 
    690                 695                 700                 


Glu Val Phe Ser Lys Tyr Tyr Thr Pro Val Leu Ala Lys Ala Val Asp 
705                 710                 715                 720 


Gly Tyr Val Lys Pro Gln Ile Lys Gln Val Val Pro Glu Phe Val Asn 
                725                 730                 735     


Ala Ser Ala Asp Ala Gly Gly Ser Ser Ala Thr Tyr Met Asp Gln Ala 
            740                 745                 750         


Pro Ser Pro Ala Val Cys Pro Gln Ala Pro Tyr Asn Met Tyr Pro Gln 
        755                 760                 765             


Asn Pro Asp His Val Leu Asp Gln Asp Gly Glu Phe Asp Leu Asp Glu 
    770                 775                 780                 


Thr Met Asp Val Ala Arg His Val Glu Glu Leu Leu Arg Arg Pro Met 
785                 790                 795                 800 


Asp Ser Leu Asp Ser Arg Leu Ser Pro Pro Ala Gly Leu Phe Thr Ser 
                805                 810                 815     


Ala Arg Gly Ser Leu Ser Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly 
            820                 825                 830         


Asp Val Glu Glu Asn Pro Gly Pro Met Ser Arg Leu Asp Lys Ser Lys 
        835                 840                 845             


Val Ile Asn Ser Ala Leu Glu Leu Leu Asn Glu Val Gly Ile Glu Gly 
    850                 855                 860                 


Leu Thr Thr Arg Lys Leu Ala Gln Lys Leu Gly Val Glu Gln Pro Thr 
865                 870                 875                 880 


Leu Tyr Trp His Val Lys Asn Lys Arg Ala Leu Leu Asp Ala Leu Ala 
                885                 890                 895     


Ile Glu Met Leu Asp Arg His His Thr His Phe Cys Pro Leu Glu Gly 
            900                 905                 910         


Glu Ser Trp Gln Asp Phe Leu Arg Asn Asn Ala Lys Ser Phe Arg Cys 
        915                 920                 925             


Ala Leu Leu Ser His Arg Asp Gly Ala Lys Val His Leu Gly Thr Arg 
    930                 935                 940                 


Pro Thr Glu Lys Gln Tyr Glu Thr Leu Glu Asn Gln Leu Ala Phe Leu 
945                 950                 955                 960 


Cys Gln Gln Gly Phe Ser Leu Glu Asn Ala Leu Tyr Ala Leu Ser Ala 
                965                 970                 975     


Val Gly His Phe Thr Leu Gly Cys Val Leu Glu Asp Gln Glu His Gln 
            980                 985                 990         


Val Ala Lys Glu Glu Arg Glu Thr  Pro Thr Thr Asp Ser  Met Pro Pro 
        995                 1000                 1005             


Leu Leu  Arg Gln Ala Ile Glu  Leu Phe Asp His Gln  Gly Ala Glu 
    1010                 1015                 1020             


Pro Ala  Phe Leu Phe Gly Leu  Glu Leu Ile Ile Cys  Gly Leu Glu 
    1025                 1030                 1035             


Lys Gln  Leu Lys Cys Glu Ser  Gly Ser Ser Gly Gly  Gly Gly Ser 
    1040                 1045                 1050             


Gly Gly  Gly Gly Ser Gly Gly  Gly Gly Ser Ala Gln  Trp Asn Gln 
    1055                 1060                 1065             


Leu Gln  Gln Leu Asp Thr Arg  Tyr Leu Glu Gln Leu  His Gln Leu 
    1070                 1075                 1080             


Tyr Ser  Asp Ser Phe Pro Met  Glu Leu Arg Gln Phe  Leu Ala Pro 
    1085                 1090                 1095             


Trp Ile  Glu Ser Gln Asp Trp  Ala Tyr Ala Ala Ser  Lys Glu Ser 
    1100                 1105                 1110             


His Ala  Thr Leu Val Phe His  Asn Leu Leu Gly Glu  Ile Asp Gln 
    1115                 1120                 1125             


Gln Tyr  Ser Arg Phe Leu Gln  Glu Ser Asn Val Leu  Tyr Gln His 
    1130                 1135                 1140             


Asn Leu  Arg Arg Ile Lys Gln  Phe Leu Gln Ser Arg  Tyr Leu Glu 
    1145                 1150                 1155             


Lys Pro  Met Glu Ile Ala Arg  Ile Val Ala Arg Cys  Leu Trp Glu 
    1160                 1165                 1170             


Glu Ser  Arg Leu Leu Gln Thr  Ala Ala Thr Ala Ala  Gln Gln Gly 
    1175                 1180                 1185             


Gly Gln  Ala Asn His Pro Thr  Ala Ala Val Val Thr  Glu Lys Gln 
    1190                 1195                 1200             


Gln Met  Leu Glu Gln His Leu  Gln Asp Val Arg Lys  Arg Val Gln 
    1205                 1210                 1215             


Asp Leu  Glu Gln Lys Met Lys  Val Val Glu Asn Leu  Gln Asp Asp 
    1220                 1225                 1230             


Phe Asp  Phe Asn Tyr Lys Thr  Leu Lys Ser Gln Gly  Asp Met Gln 
    1235                 1240                 1245             


Asp Leu  Asn Gly Asn Asn Gln  Ser Val Thr Arg Gln  Lys Met Gln 
    1250                 1255                 1260             


Gln Leu  Glu Gln Met Leu Thr  Ala Leu Asp Gln Met  Arg Arg Ser 
    1265                 1270                 1275             


Ile Val  Ser Glu Leu Ala Gly  Leu Leu Ser Ala Met  Glu Tyr Val 
    1280                 1285                 1290             


Gln Lys  Thr Leu Thr Asp Glu  Glu Leu Ala Asp Trp  Lys Arg Arg 
    1295                 1300                 1305             


Gln Gln  Ile Ala Cys Ile Gly  Gly Pro Pro Asn Ile  Cys Leu Asp 
    1310                 1315                 1320             


Arg Leu  Glu Asn Trp Ile Thr  Ser Leu Ala Glu Ser  Gln Leu Gln 
    1325                 1330                 1335             


Thr Arg  Gln Gln Ile Lys Lys  Leu Glu Glu Leu Gln  Gln Lys Val 
    1340                 1345                 1350             


Ser Tyr  Lys Gly Asp Pro Ile  Val Gln His Arg Pro  Met Leu Glu 
    1355                 1360                 1365             


Glu Arg  Ile Val Glu Leu Phe  Arg Asn Leu Met Lys  Ser Ala Phe 
    1370                 1375                 1380             


Val Val  Glu Arg Gln Pro Cys  Met Pro Met His Pro  Asp Arg Pro 
    1385                 1390                 1395             


Leu Val  Ile Lys Thr Gly Val  Gln Phe Thr Thr Lys  Val Arg Leu 
    1400                 1405                 1410             


Leu Val  Lys Phe Pro Glu Leu  Asn Tyr Gln Leu Lys  Ile Lys Val 
    1415                 1420                 1425             


Cys Ile  Asp Lys Asp Ser Gly  Asp Val Ala Ala Leu  Arg Gly Ser 
    1430                 1435                 1440             


Arg Lys  Phe Asn Ile Leu Gly  Thr Asn Thr Lys Val  Met Asn Met 
    1445                 1450                 1455             


Glu Glu  Ser Asn Asn Gly Ser  Leu Ser Ala Glu Phe  Lys His Leu 
    1460                 1465                 1470             


Thr Leu  Arg Glu Gln Arg Cys  Gly Asn Gly Gly Arg  Ala Asn Cys 
    1475                 1480                 1485             


Asp Ala  Ser Leu Ile Val Thr  Glu Glu Leu His Leu  Ile Thr Phe 
    1490                 1495                 1500             


Glu Thr  Glu Val Tyr His Gln  Gly Leu Lys Ile Asp  Leu Glu Thr 
    1505                 1510                 1515             


His Ser  Leu Pro Val Val Val  Ile Ser Asn Ile Cys  Gln Met Pro 
    1520                 1525                 1530             


Asn Ala  Trp Ala Ser Ile Leu  Trp Tyr Asn Met Leu  Thr Asn Asn 
    1535                 1540                 1545             


Pro Lys  Asn Val Asn Phe Phe  Thr Lys Pro Pro Ile  Gly Thr Trp 
    1550                 1555                 1560             


Asp Gln  Val Ala Glu Val Leu  Ser Trp Gln Phe Ser  Ser Thr Thr 
    1565                 1570                 1575             


Lys Arg  Gly Leu Ser Ile Glu  Gln Leu Thr Thr Leu  Ala Glu Lys 
    1580                 1585                 1590             


Leu Leu  Gly Pro Gly Val Asn  Tyr Ser Gly Cys Gln  Ile Thr Trp 
    1595                 1600                 1605             


Ala Lys  Phe Cys Lys Glu Asn  Met Ala Gly Lys Gly  Phe Ser Phe 
    1610                 1615                 1620             


Trp Val  Trp Leu Asp Asn Ile  Ile Asp Leu Val Lys  Lys Tyr Ile 
    1625                 1630                 1635             


Leu Ala  Leu Trp Asn Glu Gly  Tyr Ile Met Gly Phe  Ile Ser Lys 
    1640                 1645                 1650             


Glu Arg  Glu Arg Ala Ile Leu  Ser Thr Lys Pro Pro  Gly Thr Phe 
    1655                 1660                 1665             


Leu Leu  Arg Phe Ser Glu Ser  Ser Lys Glu Gly Gly  Val Thr Phe 
    1670                 1675                 1680             


Thr Trp  Val Glu Lys Asp Ile  Ser Gly Lys Thr Gln  Ile Gln Ser 
    1685                 1690                 1695             


Val Glu  Pro Tyr Thr Lys Gln  Gln Leu Asn Asn Met  Ser Phe Ala 
    1700                 1705                 1710             


Glu Ile  Ile Met Gly Tyr Lys  Ile Met Asp Ala Thr  Asn Ile Leu 
    1715                 1720                 1725             


Val Ser  Pro Leu Val Tyr Leu  Tyr Pro Asp Ile Pro  Lys Glu Glu 
    1730                 1735                 1740             


Ala Phe  Gly Lys Tyr Cys Arg  Pro Glu Ser Gln Glu  His Pro Glu 
    1745                 1750                 1755             


Ala Asp  Pro Gly Ser Ala Ala  Pro Tyr Leu Lys Thr  Lys Phe Ile 
    1760                 1765                 1770             


Cys Val  Thr Pro Thr Thr Cys  Ser Asn Thr Ile Asp  Leu Pro Met 
    1775                 1780                 1785             


Ser Pro  Arg Thr Leu Asp Ser  Leu Met Gln Phe Gly  Asn Asn Gly 
    1790                 1795                 1800             


Glu Gly  Ala Glu Pro Ser Ala  Gly Gly Gln Phe Glu  Ser Leu Thr 
    1805                 1810                 1815             


Phe Asp  Met Glu Leu Thr Ser  Glu Cys Ala Thr Ser  Pro Met 
    1820                 1825                 1830         


<210>  13
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  basic amino acid furin target sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa may be Arg or Lys

<400>  13

Arg Xaa Xaa Arg 
1               


<210>  14
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  consensus Tobacco Etch Virus (TEV) cleavage site

<400>  14

Glu Asn Leu Tyr Phe Gln Ser 
1               5           


