                         SEQUENCE LISTING

<110>  VIB VZW
       KATHOLIEKE UNIVERSITEIT LEUVEN, K.U.LEUVEN R&D
 
<120>  MEANS AND METHODS TO MODULATE PROBIOTIC POTENCY OF THE YEAST 
       SACCHAROMYCES BOULARDII

<130>  JoTh/WHI2/604

<150>  EP 17191252.0
<151>  2017-09-15

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  1461
<212>  DNA
<213>  Saccharomyces boulardii

<400>  1
atgaacgata taatcacgca agtttctcca gataatgcag agtccgctcc gattctacaa       60

gaacagcaac agcaacagaa ctcacagtac gaaggtaacg aggaggatta tggtgattca      120

ttgattcatt tgaatattca agaaaaccat tattttgtta cgagggacca gttgatgtct      180

ctacctgaat ccctattact gtgtttattt ccctcaggtg tttttttgga ccgttgtggt      240

caggtcattg ctaatttgac cagagacgat gaggtctaca ttgttaattt ccctcctgat      300

tgttttgagt acatcatgga gatatataca aaagcgcatg atgatttgta taatcatcct      360

gtggagaaat tttttgacag gccatcaagt agctttgttt cgaatgcaaa ggggtttttt      420

ggactgagta gcaataattc aatttcgagc aacaatgagc aggatatttt acatcaaaag      480

cccgctatta ttgttttgag agaagacttg gattattatt gtgtacctca ggaggaattt      540

cagtttgatt ctactaatga agaaaataat gaggatttat tgcgacattt tatggctcaa      600

gtgaaaatgg ctgctggcag ttatttaact tcaaaaacat cgattttcca aggtttgtat      660

tcttcgaata gactaaagca acaacagcaa caacagaaaa ttgaaaagga gtccaattct      720

tcttcaaata ctaaatctac ttcgaaaaaa ttgggacctg ctgaacaaca tttaatggat      780

atgttgtgct cctccggatt cactaaggaa acttgttggg gtaacagaac tcaagaaact      840

ggcaaaacgg ttataagttg actgtctctt tgccgattgg ctaacgagac aactgaagga      900

tttaggcaaa aatttaacga ggctaaggct aagtgggagg cagagcacaa accttctcaa      960

gacaacttca tcaccccaat gcaatctaac atatcgatta actctttatc tgcaagtaaa     1020

tctaacagta acatttctac agcaaggaat ttaacaagcg gaagtacagc acctgctaca     1080

gcacgtgata agagaaaatc aaggctgtcg aaactagcag ataacgttcg ttcgcactct     1140

tcctcgagac atagttcgca gaccagaagt aaacctccgg agttgcccaa attgtatgat     1200

ctagtgccaa aacctaatat caacgctaag ctactattat tttggagaaa acctgctcgt     1260

aaatgttggt ggggtgaaga agacatagag ctagaagtgg aagttttcgg ctcttggaaa     1320

gatgaatcaa agaaaatcat tgaattgatc ttgccaacaa acgttgatcc tgaagcagaa     1380

ctacataaaa tcattgtacc cgtccgatta catattcgta gagtttggac tttagagttg     1440

agcgttattg gggtgcagtg a                                               1461


<210>  2
<211>  1923
<212>  DNA
<213>  Saccharomyces boulardii

<400>  2
atgctatcgc taaaaaaatc agcgctctcc aagttgactt tgctcagaaa cacaagaaca       60

tttacatcgt cagctttggt gcgccaaacg cagggctctg taaacggttc cgcgtccaga      120

tctgcagacg ggaagtacca cataatagat cacgagtatg actgtgtggt aatcggtgcc      180

ggtggtgccg gccttagagc ggcctttggt cttgccgagg cgggctacaa gactgcttgt      240

atatccaagc ttttccccac cagatcccac actgttgctg ctcagggtgg tatcaatgcc      300

gctctgggaa atatgcacaa ggataactgg aaatggcata tgtacgatac tgtgaaagga      360

tctgattggc taggtgacca ggactccatc cattacatga ccagggaagc gcccaaatcg      420

atcattgaac tggaacacta tggtgttcct ttttcaagaa ctgaaaacgg taagatctac      480

caaagagcct ttggtggtca gaccaaggag tacggtaagg gtgctcaggc ctatagaaca      540

tgcgctgtcg cagacaggac aggacatgct cttttacaca cgctttatgg ccaagcttta      600

agatatgaca cacatttctt tattgagtac tttgccctcg atctgttgac ccataatggc      660

gaggtcgttg gtgtcatcgc ttataatcag gaagacggta ccatccacag attcagagca      720

cacaagacca ttattgccac tggtggctat ggtagagcat acttctcttg tacctctgct      780

cacacatgta cgggtgacgg taatgccatg gtttcgcgtg ctggtttccc cttgcaagat      840

ttagagtttg ttcaattcca tccttcaggt atatatgggt ctggttgctt aatcactgaa      900

ggtgctcgtg gtgaaggtgg ttttttggtt aattctgaag gtgaaagata catggaacgt      960

tacgctccta cggccaagga tctagcttgt agagatgtcg tttccagagc aatcaccatg     1020

gagatcagag aaggcagagg tgttggtaag aaaaaggacc acatgtactt acaattgagc     1080

catctacctc cggaagttct aaaggaaaga ttgccaggta tctctgaaac agcagccatt     1140

tttgctggtg tagacgtcac caaggaacct attcccatta ttcctaccgt ccactataac     1200

atgggtggta ttcccacgaa gtggaatggt gaggcattaa ccattgatga agaaactggc     1260

gaagacaagg ttattcccgg tttaatggct tgtggtgaag ccgcttgtgt ttctgtccat     1320

ggtgccaata gattaggtgc caattccttg ttggatcttg ttgtctttgg tcgtgctgtt     1380

gcccatacgg ttgctgacac tttacagcct gggttgccac acaaaccact accttctgat     1440

ttgggtaaag aatccatcgc aaacttggat aaactaagaa atgctaatgg ttcaagatct     1500

acggcagaaa ttagaatgaa tatgaaacaa actatgcaaa aggatgtttc cgtctttaga     1560

acacaatcat ctttagatga aggtgttcgg aacattactg cagtagagaa gacctttgat     1620

gatgtgaaga cgaccgatag atcaatgatc tggaattctg acttggttga aactctggag     1680

ctacagaact tattaacctg tgcctcccaa acagctgttt ccgctgctaa tagaaaggaa     1740

tcccgtggtg ctcatgcaag agaggattat ccaaatagag atgacgaaca ttggatgaag     1800

catacattat cctggcaaaa ggacgtcgct gccccagtga ctttgaaata cagaagggtt     1860

atcgatcaca ctttggacga aaaggaatgt ccttccgtac ctccaactgt aagagcctac     1920

taa                                                                   1923


<210>  3
<211>  1461
<212>  DNA
<213>  Saccharomyces boulardii

<400>  3
atggacgata taatcacgca agtttctcca gataatgcag agtccgctcc gattctacaa       60

gaacagcaac agcaacagaa ctcacagtac gaaggtaacg aggaggatta tggtgattca      120

ttgattcatt tgaatattca agaaaaccat tatttcatta cgagggacca gttgatgtct      180

ctacctgaat ccctattact gtgtttattt ccctcaggtg tttttttgga ccgttgtggt      240

caggtcatta ctaatttgac cagagacgat gaggtctaca ttgttaattt ccctcctgat      300

tgttttgagt acatcatgga gatatataca aaagcgcatg atgatttgta taatcatcct      360

gtggagaaat tttttgacag accatcaagt agctttgttt cgaatgcaaa gggatttttt      420

ggactgagta gcaataattc aatttcgagc aacaatgagc aggatatttt acatcaaaag      480

cccgctatta ttgttttgag agaagacttg gattattatt gtgtacctca ggaggaattt      540

cagtttgatt ccactaatga agaaaataat gaggatttat tgcgacattt tatggctcaa      600

gtgaaaatgg ctgctggcag ttatttaact tcaaaaacat cgattttcca aggtttgtat      660

tcttcgaata gactaaagca acaacagcaa caacagaaaa ttgaaaaggg gtccaattct      720

tcttcaaata ctaaatctac ttcgaaaaaa ttgggacctg ctgaacaaca tttaatggat      780

atgttgtgct cctccggatt caccaaggaa acttgttggg gtaacagaac tcaagaaact      840

ggcaaaacgg ttataagttc actgtctctt tgccgattgg ctaacgagac aactgaagga      900

tttaggcaaa aatttaacga ggctaaggct aagtgggagg cagagcacaa accttctcaa      960

gacaacttca tcaccccaat gcaatctaac atatcgatta actctttatc tgcaagtaaa     1020

tctaacagta ccatttctac agcaaggaat ttaacaagcg gaagtacagc acctgctaca     1080

gcacgtgata agagaaaatc aaggctgtcg aaactagcag ataacgttcg ttcgcactct     1140

tcctcgagac atagttcgca gaccagaagt aaacctccgg agttgcccaa attgtatgat     1200

ctagtgccaa aacctaatat caacgctaag ctactattat tttggagaaa acctgctcgt     1260

aaatgttggt ggggtgaaga agacatagag ctagaagtgg aagtcttcgg ctcttggaaa     1320

gatgaatcaa agaaaatcat tgaattgatc ttgccaacaa acgttgatcc tgaagcagaa     1380

ctacataaaa tcattgtacc cgtccgatta catattcgta gagtttggac tttagagttg     1440

agcgttattg gggtgcagtg a                                               1461


<210>  4
<211>  486
<212>  PRT
<213>  Saccharomyces boulardii

<400>  4

Met Asp Asp Ile Ile Thr Gln Val Ser Pro Asp Asn Ala Glu Ser Ala 
1               5                   10                  15      


Pro Ile Leu Gln Glu Gln Gln Gln Gln Gln Asn Ser Gln Tyr Glu Gly 
            20                  25                  30          


Asn Glu Glu Asp Tyr Gly Asp Ser Leu Ile His Leu Asn Ile Gln Glu 
        35                  40                  45              


Asn His Tyr Phe Ile Thr Arg Asp Gln Leu Met Ser Leu Pro Glu Ser 
    50                  55                  60                  


Leu Leu Leu Cys Leu Phe Pro Ser Gly Val Phe Leu Asp Arg Cys Gly 
65                  70                  75                  80  


Gln Val Ile Thr Asn Leu Thr Arg Asp Asp Glu Val Tyr Ile Val Asn 
                85                  90                  95      


Phe Pro Pro Asp Cys Phe Glu Tyr Ile Met Glu Ile Tyr Thr Lys Ala 
            100                 105                 110         


His Asp Asp Leu Tyr Asn His Pro Val Glu Lys Phe Phe Asp Arg Pro 
        115                 120                 125             


Ser Ser Ser Phe Val Ser Asn Ala Lys Gly Phe Phe Gly Leu Ser Ser 
    130                 135                 140                 


Asn Asn Ser Ile Ser Ser Asn Asn Glu Gln Asp Ile Leu His Gln Lys 
145                 150                 155                 160 


Pro Ala Ile Ile Val Leu Arg Glu Asp Leu Asp Tyr Tyr Cys Val Pro 
                165                 170                 175     


Gln Glu Glu Phe Gln Phe Asp Ser Thr Asn Glu Glu Asn Asn Glu Asp 
            180                 185                 190         


Leu Leu Arg His Phe Met Ala Gln Val Lys Met Ala Ala Gly Ser Tyr 
        195                 200                 205             


Leu Thr Ser Lys Thr Ser Ile Phe Gln Gly Leu Tyr Ser Ser Asn Arg 
    210                 215                 220                 


Leu Lys Gln Gln Gln Gln Gln Gln Lys Ile Glu Lys Gly Ser Asn Ser 
225                 230                 235                 240 


Ser Ser Asn Thr Lys Ser Thr Ser Lys Lys Leu Gly Pro Ala Glu Gln 
                245                 250                 255     


His Leu Met Asp Met Leu Cys Ser Ser Gly Phe Thr Lys Glu Thr Cys 
            260                 265                 270         


Trp Gly Asn Arg Thr Gln Glu Thr Gly Lys Thr Val Ile Ser Ser Leu 
        275                 280                 285             


Ser Leu Cys Arg Leu Ala Asn Glu Thr Thr Glu Gly Phe Arg Gln Lys 
    290                 295                 300                 


Phe Asn Glu Ala Lys Ala Lys Trp Glu Ala Glu His Lys Pro Ser Gln 
305                 310                 315                 320 


Asp Asn Phe Ile Thr Pro Met Gln Ser Asn Ile Ser Ile Asn Ser Leu 
                325                 330                 335     


Ser Ala Ser Lys Ser Asn Ser Thr Ile Ser Thr Ala Arg Asn Leu Thr 
            340                 345                 350         


Ser Gly Ser Thr Ala Pro Ala Thr Ala Arg Asp Lys Arg Lys Ser Arg 
        355                 360                 365             


Leu Ser Lys Leu Ala Asp Asn Val Arg Ser His Ser Ser Ser Arg His 
    370                 375                 380                 


Ser Ser Gln Thr Arg Ser Lys Pro Pro Glu Leu Pro Lys Leu Tyr Asp 
385                 390                 395                 400 


Leu Val Pro Lys Pro Asn Ile Asn Ala Lys Leu Leu Leu Phe Trp Arg 
                405                 410                 415     


Lys Pro Ala Arg Lys Cys Trp Trp Gly Glu Glu Asp Ile Glu Leu Glu 
            420                 425                 430         


Val Glu Val Phe Gly Ser Trp Lys Asp Glu Ser Lys Lys Ile Ile Glu 
        435                 440                 445             


Leu Ile Leu Pro Thr Asn Val Asp Pro Glu Ala Glu Leu His Lys Ile 
    450                 455                 460                 


Ile Val Pro Val Arg Leu His Ile Arg Arg Val Trp Thr Leu Glu Leu 
465                 470                 475                 480 


Ser Val Ile Gly Val Gln 
                485     


<210>  5
<211>  286
<212>  PRT
<213>  Saccharomyces boulardii

<400>  5

Met Asn Asp Ile Ile Thr Gln Val Ser Pro Asp Asn Ala Glu Ser Ala 
1               5                   10                  15      


Pro Ile Leu Gln Glu Gln Gln Gln Gln Gln Asn Ser Gln Tyr Glu Gly 
            20                  25                  30          


Asn Glu Glu Asp Tyr Gly Asp Ser Leu Ile His Leu Asn Ile Gln Glu 
        35                  40                  45              


Asn His Tyr Phe Val Thr Arg Asp Gln Leu Met Ser Leu Pro Glu Ser 
    50                  55                  60                  


Leu Leu Leu Cys Leu Phe Pro Ser Gly Val Phe Leu Asp Arg Cys Gly 
65                  70                  75                  80  


Gln Val Ile Ala Asn Leu Thr Arg Asp Asp Glu Val Tyr Ile Val Asn 
                85                  90                  95      


Phe Pro Pro Asp Cys Phe Glu Tyr Ile Met Glu Ile Tyr Thr Lys Ala 
            100                 105                 110         


His Asp Asp Leu Tyr Asn His Pro Val Glu Lys Phe Phe Asp Arg Pro 
        115                 120                 125             


Ser Ser Ser Phe Val Ser Asn Ala Lys Gly Phe Phe Gly Leu Ser Ser 
    130                 135                 140                 


Asn Asn Ser Ile Ser Ser Asn Asn Glu Gln Asp Ile Leu His Gln Lys 
145                 150                 155                 160 


Pro Ala Ile Ile Val Leu Arg Glu Asp Leu Asp Tyr Tyr Cys Val Pro 
                165                 170                 175     


Gln Glu Glu Phe Gln Phe Asp Ser Thr Asn Glu Glu Asn Asn Glu Asp 
            180                 185                 190         


Leu Leu Arg His Phe Met Ala Gln Val Lys Met Ala Ala Gly Ser Tyr 
        195                 200                 205             


Leu Thr Ser Lys Thr Ser Ile Phe Gln Gly Leu Tyr Ser Ser Asn Arg 
    210                 215                 220                 


Leu Lys Gln Gln Gln Gln Gln Gln Lys Ile Glu Lys Glu Ser Asn Ser 
225                 230                 235                 240 


Ser Ser Asn Thr Lys Ser Thr Ser Lys Lys Leu Gly Pro Ala Glu Gln 
                245                 250                 255     


His Leu Met Asp Met Leu Cys Ser Ser Gly Phe Thr Lys Glu Thr Cys 
            260                 265                 270         


Trp Gly Asn Arg Thr Gln Glu Thr Gly Lys Thr Val Ile Ser 
        275                 280                 285     


<210>  6
<211>  1923
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  6
atgctatcgc taaaaaaatc agcgctctcc aagttgactt tgctcagaaa cacaagaaca       60

tttacatcgt cagctttggt gcgccaaacg cagggctctg taaacggttc cgcgtccaga      120

tctgcagacg ggaagtacca cataatagat cacgagtatg actgtgtggt aatcggtgcc      180

ggtggtgccg gccttagagc ggcctttggt cttgccgagg cgggctacaa gactgcttgt      240

atatccaagc ttttccccac cagatcccac actgttgctg ctcagggtgg tatcaatgcc      300

gctctgggaa atatgcacaa ggataactgg aaatggcata tgtacgatac tgtgaaagga      360

tctgattggc taggtgacca ggactccatc cattacatga ccagggaagc gcccaaatcg      420

atcattgaac tggaacacta tggtgttcct ttttcaagaa ctgaaaacgg taagatctac      480

caaagagcct ttggtggtca gaccaaggag tacggtaagg gtgctcaggc ctatagaaca      540

tgcgctgtcg cagacaggac aggacatgct cttttacaca cgctttatgg ccaagcttta      600

agacatgaca cacatttctt tattgagtac tttgccctcg atctgttgac ccataatggc      660

gaggtcgttg gtgtcatcgc ttataatcag gaagacggta ccatccacag attcagagca      720

cacaagacca ttattgccac tggtggctat ggtagagcat acttctcttg tacctctgct      780

cacacatgta cgggtgacgg taatgccatg gtttcgcgtg ctggtttccc cttgcaagat      840

ttagagtttg ttcaattcca tccttcaggt atatatgggt ctggttgctt aatcactgaa      900

ggtgctcgtg gtgaaggtgg ttttttggtt aattctgaag gtgaaagatt catggaacgt      960

tacgctccta cggccaagga tctagcttgt agagatgtcg tttccagagc aatcaccatg     1020

gagatcagag aaggcagagg tgttggtaag aaaaaggacc acatgtactt acaattgagc     1080

catctacctc cggaagttct aaaggaaaga ttgccaggta tctctgaaac agcagccatt     1140

tttgctggtg tagacgtcac caaggaacct attcccatta ttcctaccgt ccactataac     1200

atgggtggta ttcccacgaa gtggaatggt gaggcattaa ccattgatga agaaactggc     1260

gaagacaagg ttattcccgg tttaatggct tgtggtgaag ccgcttgtgt ttctgtccat     1320

ggtgccaata gattaggtgc caattccttg ttggatcttg ttgtctttgg tcgtgctgtt     1380

gcccatacgg ttgctgacac tttacagcct gggttgccac acaaaccact accttctgat     1440

ttgggtaaag aatccatcgc aaacttggat aaactaagaa atgctaatgg ttcaagatct     1500

acggcagaaa ttagaatgaa tatgaaacaa actatgcaaa aggatgtttc cgtctttaga     1560

acacaatcat ctttagatga aggtgttcgg aacattactg cagtagagaa gacctttgat     1620

gatgtgaaga cgaccgatag atcaatgatc tggaattctg acttggttga aactctggag     1680

ctacagaact tattaacctg tgcctcccaa acagctgttt ccgctgctaa tagaaaggaa     1740

tcccgtggtg ctcatgcaag agaggattat ccaaatagag atgacgaaca ttggatgaag     1800

catacattat cctggcaaaa ggacgtcgct gccccagtga ctttgaaata cagaagggtt     1860

atcgatcaca ctttggacga aaaggaatgt ccttccgtac ctccaactgt aagagcctac     1920

taa                                                                   1923


<210>  7
<211>  640
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  7

Met Leu Ser Leu Lys Lys Ser Ala Leu Ser Lys Leu Thr Leu Leu Arg 
1               5                   10                  15      


Asn Thr Arg Thr Phe Thr Ser Ser Ala Leu Val Arg Gln Thr Gln Gly 
            20                  25                  30          


Ser Val Asn Gly Ser Ala Ser Arg Ser Ala Asp Gly Lys Tyr His Ile 
        35                  40                  45              


Ile Asp His Glu Tyr Asp Cys Val Val Ile Gly Ala Gly Gly Ala Gly 
    50                  55                  60                  


Leu Arg Ala Ala Phe Gly Leu Ala Glu Ala Gly Tyr Lys Thr Ala Cys 
65                  70                  75                  80  


Ile Ser Lys Leu Phe Pro Thr Arg Ser His Thr Val Ala Ala Gln Gly 
                85                  90                  95      


Gly Ile Asn Ala Ala Leu Gly Asn Met His Lys Asp Asn Trp Lys Trp 
            100                 105                 110         


His Met Tyr Asp Thr Val Lys Gly Ser Asp Trp Leu Gly Asp Gln Asp 
        115                 120                 125             


Ser Ile His Tyr Met Thr Arg Glu Ala Pro Lys Ser Ile Ile Glu Leu 
    130                 135                 140                 


Glu His Tyr Gly Val Pro Phe Ser Arg Thr Glu Asn Gly Lys Ile Tyr 
145                 150                 155                 160 


Gln Arg Ala Phe Gly Gly Gln Thr Lys Glu Tyr Gly Lys Gly Ala Gln 
                165                 170                 175     


Ala Tyr Arg Thr Cys Ala Val Ala Asp Arg Thr Gly His Ala Leu Leu 
            180                 185                 190         


His Thr Leu Tyr Gly Gln Ala Leu Arg His Asp Thr His Phe Phe Ile 
        195                 200                 205             


Glu Tyr Phe Ala Leu Asp Leu Leu Thr His Asn Gly Glu Val Val Gly 
    210                 215                 220                 


Val Ile Ala Tyr Asn Gln Glu Asp Gly Thr Ile His Arg Phe Arg Ala 
225                 230                 235                 240 


His Lys Thr Ile Ile Ala Thr Gly Gly Tyr Gly Arg Ala Tyr Phe Ser 
                245                 250                 255     


Cys Thr Ser Ala His Thr Cys Thr Gly Asp Gly Asn Ala Met Val Ser 
            260                 265                 270         


Arg Ala Gly Phe Pro Leu Gln Asp Leu Glu Phe Val Gln Phe His Pro 
        275                 280                 285             


Ser Gly Ile Tyr Gly Ser Gly Cys Leu Ile Thr Glu Gly Ala Arg Gly 
    290                 295                 300                 


Glu Gly Gly Phe Leu Val Asn Ser Glu Gly Glu Arg Phe Met Glu Arg 
305                 310                 315                 320 


Tyr Ala Pro Thr Ala Lys Asp Leu Ala Cys Arg Asp Val Val Ser Arg 
                325                 330                 335     


Ala Ile Thr Met Glu Ile Arg Glu Gly Arg Gly Val Gly Lys Lys Lys 
            340                 345                 350         


Asp His Met Tyr Leu Gln Leu Ser His Leu Pro Pro Glu Val Leu Lys 
        355                 360                 365             


Glu Arg Leu Pro Gly Ile Ser Glu Thr Ala Ala Ile Phe Ala Gly Val 
    370                 375                 380                 


Asp Val Thr Lys Glu Pro Ile Pro Ile Ile Pro Thr Val His Tyr Asn 
385                 390                 395                 400 


Met Gly Gly Ile Pro Thr Lys Trp Asn Gly Glu Ala Leu Thr Ile Asp 
                405                 410                 415     


Glu Glu Thr Gly Glu Asp Lys Val Ile Pro Gly Leu Met Ala Cys Gly 
            420                 425                 430         


Glu Ala Ala Cys Val Ser Val His Gly Ala Asn Arg Leu Gly Ala Asn 
        435                 440                 445             


Ser Leu Leu Asp Leu Val Val Phe Gly Arg Ala Val Ala His Thr Val 
    450                 455                 460                 


Ala Asp Thr Leu Gln Pro Gly Leu Pro His Lys Pro Leu Pro Ser Asp 
465                 470                 475                 480 


Leu Gly Lys Glu Ser Ile Ala Asn Leu Asp Lys Leu Arg Asn Ala Asn 
                485                 490                 495     


Gly Ser Arg Ser Thr Ala Glu Ile Arg Met Asn Met Lys Gln Thr Met 
            500                 505                 510         


Gln Lys Asp Val Ser Val Phe Arg Thr Gln Ser Ser Leu Asp Glu Gly 
        515                 520                 525             


Val Arg Asn Ile Thr Ala Val Glu Lys Thr Phe Asp Asp Val Lys Thr 
    530                 535                 540                 


Thr Asp Arg Ser Met Ile Trp Asn Ser Asp Leu Val Glu Thr Leu Glu 
545                 550                 555                 560 


Leu Gln Asn Leu Leu Thr Cys Ala Ser Gln Thr Ala Val Ser Ala Ala 
                565                 570                 575     


Asn Arg Lys Glu Ser Arg Gly Ala His Ala Arg Glu Asp Tyr Pro Asn 
            580                 585                 590         


Arg Asp Asp Glu His Trp Met Lys His Thr Leu Ser Trp Gln Lys Asp 
        595                 600                 605             


Val Ala Ala Pro Val Thr Leu Lys Tyr Arg Arg Val Ile Asp His Thr 
    610                 615                 620                 


Leu Asp Glu Lys Glu Cys Pro Ser Val Pro Pro Thr Val Arg Ala Tyr 
625                 630                 635                 640 


<210>  8
<211>  640
<212>  PRT
<213>  Saccharomyces boulardii

<400>  8

Met Leu Ser Leu Lys Lys Ser Ala Leu Ser Lys Leu Thr Leu Leu Arg 
1               5                   10                  15      


Asn Thr Arg Thr Phe Thr Ser Ser Ala Leu Val Arg Gln Thr Gln Gly 
            20                  25                  30          


Ser Val Asn Gly Ser Ala Ser Arg Ser Ala Asp Gly Lys Tyr His Ile 
        35                  40                  45              


Ile Asp His Glu Tyr Asp Cys Val Val Ile Gly Ala Gly Gly Ala Gly 
    50                  55                  60                  


Leu Arg Ala Ala Phe Gly Leu Ala Glu Ala Gly Tyr Lys Thr Ala Cys 
65                  70                  75                  80  


Ile Ser Lys Leu Phe Pro Thr Arg Ser His Thr Val Ala Ala Gln Gly 
                85                  90                  95      


Gly Ile Asn Ala Ala Leu Gly Asn Met His Lys Asp Asn Trp Lys Trp 
            100                 105                 110         


His Met Tyr Asp Thr Val Lys Gly Ser Asp Trp Leu Gly Asp Gln Asp 
        115                 120                 125             


Ser Ile His Tyr Met Thr Arg Glu Ala Pro Lys Ser Ile Ile Glu Leu 
    130                 135                 140                 


Glu His Tyr Gly Val Pro Phe Ser Arg Thr Glu Asn Gly Lys Ile Tyr 
145                 150                 155                 160 


Gln Arg Ala Phe Gly Gly Gln Thr Lys Glu Tyr Gly Lys Gly Ala Gln 
                165                 170                 175     


Ala Tyr Arg Thr Cys Ala Val Ala Asp Arg Thr Gly His Ala Leu Leu 
            180                 185                 190         


His Thr Leu Tyr Gly Gln Ala Leu Arg Tyr Asp Thr His Phe Phe Ile 
        195                 200                 205             


Glu Tyr Phe Ala Leu Asp Leu Leu Thr His Asn Gly Glu Val Val Gly 
    210                 215                 220                 


Val Ile Ala Tyr Asn Gln Glu Asp Gly Thr Ile His Arg Phe Arg Ala 
225                 230                 235                 240 


His Lys Thr Ile Ile Ala Thr Gly Gly Tyr Gly Arg Ala Tyr Phe Ser 
                245                 250                 255     


Cys Thr Ser Ala His Thr Cys Thr Gly Asp Gly Asn Ala Met Val Ser 
            260                 265                 270         


Arg Ala Gly Phe Pro Leu Gln Asp Leu Glu Phe Val Gln Phe His Pro 
        275                 280                 285             


Ser Gly Ile Tyr Gly Ser Gly Cys Leu Ile Thr Glu Gly Ala Arg Gly 
    290                 295                 300                 


Glu Gly Gly Phe Leu Val Asn Ser Glu Gly Glu Arg Tyr Met Glu Arg 
305                 310                 315                 320 


Tyr Ala Pro Thr Ala Lys Asp Leu Ala Cys Arg Asp Val Val Ser Arg 
                325                 330                 335     


Ala Ile Thr Met Glu Ile Arg Glu Gly Arg Gly Val Gly Lys Lys Lys 
            340                 345                 350         


Asp His Met Tyr Leu Gln Leu Ser His Leu Pro Pro Glu Val Leu Lys 
        355                 360                 365             


Glu Arg Leu Pro Gly Ile Ser Glu Thr Ala Ala Ile Phe Ala Gly Val 
    370                 375                 380                 


Asp Val Thr Lys Glu Pro Ile Pro Ile Ile Pro Thr Val His Tyr Asn 
385                 390                 395                 400 


Met Gly Gly Ile Pro Thr Lys Trp Asn Gly Glu Ala Leu Thr Ile Asp 
                405                 410                 415     


Glu Glu Thr Gly Glu Asp Lys Val Ile Pro Gly Leu Met Ala Cys Gly 
            420                 425                 430         


Glu Ala Ala Cys Val Ser Val His Gly Ala Asn Arg Leu Gly Ala Asn 
        435                 440                 445             


Ser Leu Leu Asp Leu Val Val Phe Gly Arg Ala Val Ala His Thr Val 
    450                 455                 460                 


Ala Asp Thr Leu Gln Pro Gly Leu Pro His Lys Pro Leu Pro Ser Asp 
465                 470                 475                 480 


Leu Gly Lys Glu Ser Ile Ala Asn Leu Asp Lys Leu Arg Asn Ala Asn 
                485                 490                 495     


Gly Ser Arg Ser Thr Ala Glu Ile Arg Met Asn Met Lys Gln Thr Met 
            500                 505                 510         


Gln Lys Asp Val Ser Val Phe Arg Thr Gln Ser Ser Leu Asp Glu Gly 
        515                 520                 525             


Val Arg Asn Ile Thr Ala Val Glu Lys Thr Phe Asp Asp Val Lys Thr 
    530                 535                 540                 


Thr Asp Arg Ser Met Ile Trp Asn Ser Asp Leu Val Glu Thr Leu Glu 
545                 550                 555                 560 


Leu Gln Asn Leu Leu Thr Cys Ala Ser Gln Thr Ala Val Ser Ala Ala 
                565                 570                 575     


Asn Arg Lys Glu Ser Arg Gly Ala His Ala Arg Glu Asp Tyr Pro Asn 
            580                 585                 590         


Arg Asp Asp Glu His Trp Met Lys His Thr Leu Ser Trp Gln Lys Asp 
        595                 600                 605             


Val Ala Ala Pro Val Thr Leu Lys Tyr Arg Arg Val Ile Asp His Thr 
    610                 615                 620                 


Leu Asp Glu Lys Glu Cys Pro Ser Val Pro Pro Thr Val Arg Ala Tyr 
625                 630                 635                 640 


<210>  9
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gRNA recognition sites

<400>  9
ggctgatttt cgcagttcgg ggg                                               23


<210>  10
<211>  35
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  10

Cys Ala Val Ala Asp Arg Thr Gly His Ala Leu Leu His Thr Leu Tyr 
1               5                   10                  15      


Gly Gln Ala Leu Arg His Asp Thr His Phe Glu Gly Glu Arg Phe Met 
            20                  25                  30          


Glu Arg Tyr 
        35  


<210>  11
<211>  35
<212>  PRT
<213>  Saccharomyces boulardii

<400>  11

Cys Ala Val Ala Asp Arg Thr Gly His Ala Leu Leu His Thr Leu Tyr 
1               5                   10                  15      


Gly Gln Ala Leu Arg Tyr Asp Thr His Phe Glu Gly Glu Arg Tyr Met 
            20                  25                  30          


Glu Arg Tyr 
        35  


<210>  12
<211>  35
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  12

Cys Ala Val Ala Asp Arg Thr Gly His Ala Leu Leu His Thr Leu Tyr 
1               5                   10                  15      


Gly Gln Ala Leu Arg Tyr Asp Thr His Phe Glu Gly Glu Arg Phe Met 
            20                  25                  30          


Glu Arg Tyr 
        35  


