
                                SEQUENCE LISTING

<110> Fred Hutchinson Cancer Research Center
      Technische Universitat Munchen
      Liu, Lingfeng
      Riddell, Stanley R.
      Busch, Dirk
      Frassle, Simon

<120> STREP-TAG SPECIFIC CHIMERIC RECEPTORS AND USES THEROF

<130> 360056.450WO

<140> PCT
<141> 2018-09-06

<150> US 62/555,012
<151> 2017-09-06

<160> 49

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 360
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 3E8 VH

<400> 1
gaggtgcagc tggtggagac tgggggaggc tttgtgaagc ctggaggctc cctgaaactc      60
tcctgtgcag cctctggatt cactttcagt agttatggca tgtcttgggt tcgccagact     120
ccggagaaga ggctggagtg ggtcgcagcc atcaccagtg atggcggtgg cacccactat     180
ccagatactg tgaagggccg attcaccatc tccagagact ttgccaaaaa caccctgtac     240
ctgcagatga gcagtctgag gtctgaggac acagcctggt atttctgtgc aagacatgag     300
ccccgactga tagcctggtt tgctcactgg ggccaaggaa ctctggtcac tgtctctgca     360

<210> 2
<211> 120
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 3E8 VH

<400> 2
Glu Val Gln Leu Val Glu Thr Gly Gly Gly Phe Val Lys Pro Gly Gly
 1               5                  10                  15
Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
            20                  25                  30
Gly Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val
        35                  40                  45
Ala Ala Ile Thr Ser Asp Gly Gly Gly Thr His Tyr Pro Asp Thr Val
    50                  55                  60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Phe Ala Lys Asn Thr Leu Tyr
65                  70                  75                  80
Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Trp Tyr Phe Cys
                85                  90                  95
Ala Arg His Glu Pro Arg Leu Ile Ala Trp Phe Ala His Trp Gly Gln
            100                 105                 110
Gly Thr Leu Val Thr Val Ser Ala
        115                 120

<210> 3
<211> 112
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 3E8 VL

<400> 3
Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly
 1               5                  10                  15
Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser
            20                  25                  30
Asn Gly Tyr Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser
        35                  40                  45
Pro Lys Leu Leu Ile Tyr Glu Val Ser Asn Arg Phe Ser Gly Val Pro
    50                  55                  60
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
65                  70                  75                  80
Ile Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly
                85                  90                  95
Ser His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys
            100                 105                 110

<210> 4
<211> 337
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 3E8 VL

<400> 4
gatgttttga tgacccaaac tccactctcc ctgcctgtca gtcttggaga tcaagcctct      60
atctcttgca gatctagtca gagcattgtt catagtaatg gatacaccta tttagaatgg     120
tacctgcaga aaccaggcca gtctccaaag ctcctgatct acgaagtttc caaccgattt     180
tctggggtcc cagacaggtt cagtggcagt ggatcaggga cagatttcac actcaagatc     240
atcagagtgg aggctgagga tctgggagtt tattattgct ttcaaggttc acatgttccg     300
tggacgttcg gtggaggcac caagctggaa atcaaac                              337

<210> 5
<211> 247
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII 3E8 scFv

<400> 5
Glu Val Gln Leu Val Glu Thr Gly Gly Gly Phe Val Lys Pro Gly Gly
 1               5                  10                  15
Ser Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr
            20                  25                  30
Gly Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val
        35                  40                  45
Ala Ala Ile Thr Ser Asp Gly Gly Gly Thr His Tyr Pro Asp Thr Val
    50                  55                  60
Lys Gly Arg Phe Thr Ile Ser Arg Asp Phe Ala Lys Asn Thr Leu Tyr
65                  70                  75                  80
Leu Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Trp Tyr Phe Cys
                85                  90                  95
Ala Arg His Glu Pro Arg Leu Ile Ala Trp Phe Ala His Trp Gly Gln
            100                 105                 110
Gly Thr Leu Val Thr Val Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly
        115                 120                 125
Gly Ser Gly Gly Gly Gly Ser Asp Val Leu Met Thr Gln Thr Pro Leu
    130                 135                 140
Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser
145                 150                 155                 160
Ser Gln Ser Ile Val His Ser Asn Gly Tyr Thr Tyr Leu Glu Trp Tyr
                165                 170                 175
Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Glu Val Ser
            180                 185                 190
Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly
        195                 200                 205
Thr Asp Phe Thr Leu Lys Ile Ile Arg Val Glu Ala Glu Asp Leu Gly
    210                 215                 220
Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Trp Thr Phe Gly Gly
225                 230                 235                 240
Gly Thr Lys Leu Glu Ile Lys
                245

<210> 6
<211> 247
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII 3E8 scFv

<400> 6
Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly
 1               5                  10                  15
Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser
            20                  25                  30
Asn Gly Tyr Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser
        35                  40                  45
Pro Lys Leu Leu Ile Tyr Glu Val Ser Asn Arg Phe Ser Gly Val Pro
    50                  55                  60
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
65                  70                  75                  80
Ile Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly
                85                  90                  95
Ser His Val Pro Trp Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys
            100                 105                 110
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu
        115                 120                 125
Val Gln Leu Val Glu Thr Gly Gly Gly Phe Val Lys Pro Gly Gly Ser
    130                 135                 140
Leu Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly
145                 150                 155                 160
Met Ser Trp Val Arg Gln Thr Pro Glu Lys Arg Leu Glu Trp Val Ala
                165                 170                 175
Ala Ile Thr Ser Asp Gly Gly Gly Thr His Tyr Pro Asp Thr Val Lys
            180                 185                 190
Gly Arg Phe Thr Ile Ser Arg Asp Phe Ala Lys Asn Thr Leu Tyr Leu
        195                 200                 205
Gln Met Ser Ser Leu Arg Ser Glu Asp Thr Ala Trp Tyr Phe Cys Ala
    210                 215                 220
Arg His Glu Pro Arg Leu Ile Ala Trp Phe Ala His Trp Gly Gln Gly
225                 230                 235                 240
Thr Leu Val Thr Val Ser Ala
                245

<210> 7
<211> 360
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 5G2 VH

<400> 7
caggttcaac tgcagcagtc tggagctgag ctggcgaggc caggggcttc agtgaagctg      60
tcctgcacgg cttctggata caccttcaca agctatggta taacctgggt gaggcagaga     120
actggacagg gccttgagtg gattggagag atttttcctg gaagtggtga tacttcctac     180
ggtgagaaat tcaagggcca ggccacactg actacagaca aatcctccag cacagcctac     240
atgcagctca gcagcctgac atctgaggac tctgcagtct atttctgtgc aagacgctat     300
aggtacattt accatgctat ggactactgg ggtcaaggaa cctcagtcac cgtctcctca     360

<210> 8
<211> 120
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 5G2 VH

<400> 8
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala
 1               5                  10                  15
Ser Val Lys Leu Ser Cys Thr Ala Ser Gly Tyr Thr Phe Thr Ser Tyr
            20                  25                  30
Gly Ile Thr Trp Val Arg Gln Arg Thr Gly Gln Gly Leu Glu Trp Ile
        35                  40                  45
Gly Glu Ile Phe Pro Gly Ser Gly Asp Thr Ser Tyr Gly Glu Lys Phe
    50                  55                  60
Lys Gly Gln Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr
65                  70                  75                  80
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys
                85                  90                  95
Ala Arg Arg Tyr Arg Tyr Ile Tyr His Ala Met Asp Tyr Trp Gly Gln
            100                 105                 110
Gly Thr Ser Val Thr Val Ser Ser
        115                 120

<210> 9
<211> 336
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 5G2 VL

<400> 9
gatattttga tgacccaaac tccactctcc ctgcctgtca gtcttggaga tcaagcctcc      60
atctcttgca gatctagtca gagcattgta catagtaatg gcaacaccta tttagaatgg     120
tacctgcaga aaccaggcca gtctccaaag ctcctgatct acaaagtttc caaccgattt     180
tctggggtcc cagacaggtt cagtggcagt ggatcaggga cagatttcac actcaagatc     240
cgcagagtgg aggctgagga tctgggagtt tattactgct ttcaaggttc acatgttccg     300
ctcacgttcg gtgctgggac caagctggag ctgaaa                               336

<210> 10
<211> 112
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 5G2 VL

<400> 10
Asp Ile Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly
 1               5                  10                  15
Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser
            20                  25                  30
Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser
        35                  40                  45
Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro
    50                  55                  60
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
65                  70                  75                  80
Arg Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly
                85                  90                  95
Ser His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
            100                 105                 110

<210> 11
<211> 247
<212> PRT
<213> qArtificial Sequence

<220> 
<223> Synthetic sequence Anti-STII 5G2 scFv

<400> 11
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala
 1               5                  10                  15
Ser Val Lys Leu Ser Cys Thr Ala Ser Gly Tyr Thr Phe Thr Ser Tyr
            20                  25                  30
Gly Ile Thr Trp Val Arg Gln Arg Thr Gly Gln Gly Leu Glu Trp Ile
        35                  40                  45
Gly Glu Ile Phe Pro Gly Ser Gly Asp Thr Ser Tyr Gly Glu Lys Phe
    50                  55                  60
Lys Gly Gln Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr
65                  70                  75                  80
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys
                85                  90                  95
Ala Arg Arg Tyr Arg Tyr Ile Tyr His Ala Met Asp Tyr Trp Gly Gln
            100                 105                 110
Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly
        115                 120                 125
Gly Ser Gly Gly Gly Gly Ser Asp Ile Leu Met Thr Gln Thr Pro Leu
    130                 135                 140
Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser
145                 150                 155                 160
Ser Gln Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Tyr
                165                 170                 175
Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser
            180                 185                 190
Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly
        195                 200                 205
Thr Asp Phe Thr Leu Lys Ile Arg Arg Val Glu Ala Glu Asp Leu Gly
    210                 215                 220
Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Leu Thr Phe Gly Ala
225                 230                 235                 240
Gly Thr Lys Leu Glu Leu Lys
                245

<210> 12
<211> 247
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII 5G2 scFv

<400> 12
Asp Ile Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly
 1               5                  10                  15
Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser
            20                  25                  30
Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser
        35                  40                  45
Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro
    50                  55                  60
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
65                  70                  75                  80
Arg Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly
                85                  90                  95
Ser His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
            100                 105                 110
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln
        115                 120                 125
Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser
    130                 135                 140
Val Lys Leu Ser Cys Thr Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Gly
145                 150                 155                 160
Ile Thr Trp Val Arg Gln Arg Thr Gly Gln Gly Leu Glu Trp Ile Gly
                165                 170                 175
Glu Ile Phe Pro Gly Ser Gly Asp Thr Ser Tyr Gly Glu Lys Phe Lys
            180                 185                 190
Gly Gln Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr Met
        195                 200                 205
Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
    210                 215                 220
Arg Arg Tyr Arg Tyr Ile Tyr His Ala Met Asp Tyr Trp Gly Gln Gly
225                 230                 235                 240
Thr Ser Val Thr Val Ser Ser
                245

<210> 13
<211> 360
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 4E2 VH

<400> 13
caggttcaac tgcagcagtc tggagctgag ctggcgaggc caggggcttc agtgaagctg      60
tcctgcacgg cttctggata caccttcaca agctatggta taacctgggt gaggcagaga     120
actggacagg gccttgagtg gattggagag atttttcctg gaagtggtga tacttcctac     180
ggtgagaaat taaagggcca ggccacactg actacagaca aatcctccag cacagcctac     240
atgcagctca gcagcctgac atctgaggac tctgcagtct atttctgtgc aagacgctat     300
aggtacattt accatgctat ggactactgg ggtcaaggaa cctcagtcac cgtctcctca     360

<210> 14
<211> 120
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 4E2 VH

<400> 14
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala
 1               5                  10                  15
Ser Val Lys Leu Ser Cys Thr Ala Ser Gly Tyr Thr Phe Thr Ser Tyr
            20                  25                  30
Gly Ile Thr Trp Val Arg Gln Arg Thr Gly Gln Gly Leu Glu Trp Ile
        35                  40                  45
Gly Glu Ile Phe Pro Gly Ser Gly Asp Thr Ser Tyr Gly Glu Lys Leu
    50                  55                  60
Lys Gly Gln Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr
65                  70                  75                  80
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys
                85                  90                  95
Ala Arg Arg Tyr Arg Tyr Ile Tyr His Ala Met Asp Tyr Trp Gly Gln
            100                 105                 110
Gly Thr Ser Val Thr Val Ser Ser
        115                 120

<210> 15
<211> 336
<212> DNA
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 4E2 VL

<400> 15
gatattttga tgacccaaac tccactctcc ctgcctgtca gtcttggaga tcaagcctcc      60
atctcttgca gatctagtca gagcattgta catagtaatg gcaacaccta tttagagtgg     120
tacctgcaga aaccaggcca gtctccaaag ctcctgatct acaaagtttc caaccgattt     180
tctggggtcc cagacaggtt cagtggcagt ggatcaggga cagatttcac actcaagatc     240
agcagagtgg aggctgagga tctgggagtt tattactgct ttcaaggttc acatgttccg     300
ctcacgttcg gtgctgggac caagctggag ctgaaa                               336

<210> 16
<211> 112
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII mAb 4E2 VL

<400> 16
Asp Ile Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly
 1               5                  10                  15
Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser
            20                  25                  30
Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser
        35                  40                  45
Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro
    50                  55                  60
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
65                  70                  75                  80
Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly
                85                  90                  95
Ser His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
            100                 105                 110

<210> 17
<211> 247
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII 4E2 scFv

<400> 17
Gln Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala
 1               5                  10                  15
Ser Val Lys Leu Ser Cys Thr Ala Ser Gly Tyr Thr Phe Thr Ser Tyr
            20                  25                  30
Gly Ile Thr Trp Val Arg Gln Arg Thr Gly Gln Gly Leu Glu Trp Ile
        35                  40                  45
Gly Glu Ile Phe Pro Gly Ser Gly Asp Thr Ser Tyr Gly Glu Lys Leu
    50                  55                  60
Lys Gly Gln Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr
65                  70                  75                  80
Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys
                85                  90                  95
Ala Arg Arg Tyr Arg Tyr Ile Tyr His Ala Met Asp Tyr Trp Gly Gln
            100                 105                 110
Gly Thr Ser Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly
        115                 120                 125
Gly Ser Gly Gly Gly Gly Ser Asp Ile Leu Met Thr Gln Thr Pro Leu
    130                 135                 140
Ser Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser
145                 150                 155                 160
Ser Gln Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Tyr
                165                 170                 175
Leu Gln Lys Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser
            180                 185                 190
Asn Arg Phe Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly
        195                 200                 205
Thr Asp Phe Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly
    210                 215                 220
Val Tyr Tyr Cys Phe Gln Gly Ser His Val Pro Leu Thr Phe Gly Ala
225                 230                 235                 240
Gly Thr Lys Leu Glu Leu Lys
                245

<210> 18
<211> 247
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Anti-STII 4E2 scFv

<400> 18
Asp Ile Leu Met Thr Gln Thr Pro Leu Ser Leu Pro Val Ser Leu Gly
 1               5                  10                  15
Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser Gln Ser Ile Val His Ser
            20                  25                  30
Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys Pro Gly Gln Ser
        35                  40                  45
Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe Ser Gly Val Pro
    50                  55                  60
Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile
65                  70                  75                  80
Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Phe Gln Gly
                85                  90                  95
Ser His Val Pro Leu Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys
            100                 105                 110
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gln
        115                 120                 125
Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Ala Arg Pro Gly Ala Ser
    130                 135                 140
Val Lys Leu Ser Cys Thr Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Gly
145                 150                 155                 160
Ile Thr Trp Val Arg Gln Arg Thr Gly Gln Gly Leu Glu Trp Ile Gly
                165                 170                 175
Glu Ile Phe Pro Gly Ser Gly Asp Thr Ser Tyr Gly Glu Lys Leu Lys
            180                 185                 190
Gly Gln Ala Thr Leu Thr Thr Asp Lys Ser Ser Ser Thr Ala Tyr Met
        195                 200                 205
Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Phe Cys Ala
    210                 215                 220
Arg Arg Tyr Arg Tyr Ile Tyr His Ala Met Asp Tyr Trp Gly Gln Gly
225                 230                 235                 240
Thr Ser Val Thr Val Ser Ser
                245

<210> 19
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Strep-Tag II

<400> 19
Trp Ser His Pro Gln Phe Glu Lys
 1               5

<210> 20
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence (Gly4Ser)2 linker

<400> 20
Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser
 1               5                  10

<210> 21
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence (Gly3Ser)2 linker

<400> 21
Gly Gly Gly Ser Gly Gly Gly Ser
 1               5

<210> 22
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 3E8 HCDR1 amino acid

<400> 22
Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr Gly Met Ser
 1               5                  10

<210> 23
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 3E3 HCDR2 amino acid

<400> 23
Ala Ile Thr Ser Asp Gly Gly Gly Thr His
 1               5                  10

<210> 24
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 3E8 HCDR3

<400> 24
Ala Arg His Glu Pro Arg Leu Ile Ala Trp Phe Ala His
 1               5                  10

<210> 25
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 3E8 LCDR1

<400> 25
Arg Ser Ser Gln Ser Ile Val His Ser Asn Gly Tyr Thr Tyr Leu Glu
 1               5                  10                  15

<210> 26
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 3E8 LCDR2

<400> 26
Tyr Glu Val Ser Asn Arg Phe Ser
 1               5

<210> 27
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 3E8 LCDR3

<400> 27
Phe Gln Gly Ser His Val Pro Trp Thr
 1               5

<210> 28
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 5G2 HCDR1

<400> 28
Thr Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Gly Ile Thr
 1               5                  10

<210> 29
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 5G2 HCDR2

<400> 29
Glu Ile Phe Pro Gly Ser Gly Asp Thr Ser
 1               5                  10

<210> 30
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 5G2 HCDR3

<400> 30
Ala Arg Arg Tyr Arg Tyr Ile Tyr His Ala Met Asp Tyr
 1               5                  10

<210> 31
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 5G2 LCDR1

<400> 31
Arg Ser Ser Gln Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu
 1               5                  10                  15

<210> 32
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 5G2 LCDR2

<400> 32
Tyr Lys Val Ser Asn Arg Phe Ser
 1               5

<210> 33
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 5G2 LCDR3

<400> 33
Phe Gln Gly Ser His Val Pro Leu Thr
 1               5

<210> 34
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 4E2 HCDR1

<400> 34
Thr Ala Ser Gly Tyr Thr Phe Thr Ser Tyr Gly Ile Thr
 1               5                  10

<210> 35
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 4E2 HCDR2

<400> 35
Glu Ile Phe Pro Gly Ser Gly Asp Thr Ser
 1               5                  10

<210> 36
<211> 13
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 4E2  HCDR3

<400> 36
Ala Arg Arg Tyr Arg Tyr Ile Tyr His Ala Met Asp Tyr
 1               5                  10

<210> 37
<211> 16
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 4E2  LCDR1

<400> 37
Arg Ser Ser Gln Ser Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu
 1               5                  10                  15

<210> 38
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 4E2 LCDR2

<400> 38
Tyr Lys Val Ser Asn Arg Phe Ser
 1               5

<210> 39
<211> 9
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence 4E2  LCDR3

<400> 39
Phe Gln Gly Ser His Val Pro Leu Thr
 1               5

<210> 40
<211> 19
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence P2A

<400> 40
Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val Glu Glu Asn
 1               5                  10                  15
Pro Gly Pro


<210> 41
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence P2A mod

<400> 41
Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val
 1               5                  10                  15
Glu Glu Asn Pro Gly Pro
            20

<210> 42
<211> 18
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence T2A

<400> 42
Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro
 1               5                  10                  15
Gly Pro


<210> 43
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence T2A mod

<400> 43
Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu
 1               5                  10                  15
Glu Asn Pro Gly Pro
            20

<210> 44
<211> 21
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence E2A

<400> 44
Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Ser Asp Val Glu
 1               5                  10                  15
Ser Asn Pro Gly Pro
            20

<210> 45
<211> 24
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence E2A mod

<400> 45
Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Ser
 1               5                  10                  15
Asp Val Glu Ser Asn Pro Gly Pro
            20

<210> 46
<211> 22
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence F2A

<400> 46
Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala Gly Asp Val
 1               5                  10                  15
Glu Ser Asn Pro Gly Pro
            20

<210> 47
<211> 25
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence F2A mod

<400> 47
Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala
 1               5                  10                  15
Gly Asp Val Glu Ser Asn Pro Gly Pro
            20                  25

<210> 48
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence Strep-tag

<400> 48
Trp Arg His Pro Gln Phe Gly Gly
 1               5

<210> 49
<211> 11
<212> PRT
<213> Artificial Sequence

<220> 
<223> Synthetic sequence (Gly3Ser)2Gly2Ser linker

<400> 49
Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Ser
 1               5                  10
