                         SEQUENCE LISTING

<110>  BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA
       TOKAI UNIVERSITY EDUCATIONAL SYSTEM
       GURUMURTHY, Channabasavaiah
       OHTSUKA, Masato
       SATO, Masahiro
 
<120>  METHODS FOR IN SITU GERMLINE GENOME ENGINEERING

<130>  6278-00007

<150>  US 62/549,644
<151>  2017-08-24

<160>  69    

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Foxe3 Target Sequence

<400>  1
cctgagacag ccgggcttcg cgccggggc                                         29


<210>  2
<211>  79
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: WT

<400>  2
ctcggagccg gggaagccta cctgagacag ccgggcttcg cgccggggct ggagcgctac       60

ctgtgagtcg ctttccgct                                                    79


<210>  3
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: #1

<400>  3
ctcggagccg gggaagccta cctgagacag ccgagcgcta cctgtgagtc gctttccgct       60


<210>  4
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: #3

<400>  4
ctcggaagct ggagcgctac ctgtgagtcg ctttccgct                              39


<210>  5
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: #5

<400>  5
ctcggagccg gggaagccta cctgagacag ccgggcttcg gcgccggggc tggagcgcta       60

cctgtgagtc gctttccgct                                                   80


<210>  6
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: #8

<400>  6
ctcggagccg gggaagccta cctgagactg agtcgctttc cgct                        44


<210>  7
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: #9

<400>  7
ctcggagccg gggaagccta cctgagacag ccgggcttcg cggggctgga gcgctacctg       60

tgagtcgctt tccgct                                                       76


<210>  8
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: #16

<400>  8
ctcggagccg gggaagccta cctgagacag ccgggcttcg cggggctgga gcgctacctg       60

tgagtcgctt tccgct                                                       76


<210>  9
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: #24

<400>  9
ctcggagccg gggaagccta cctgctttcc gct                                    33


<210>  10
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: #26

<400>  10
ctcggagccg gggaagccta cctgagacag ccgggcttcg agcgctacct gtgagtcgct       60

ttccgct                                                                 67


<210>  11
<211>  54
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: #32

<400>  11
ctcggagccg gggaagccta cctgagacag cctacctgtg agtcgctttc cgct             54


<210>  12
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Fig. 3 Target

<400>  12
tgcggaaact ctaagtttgg atttggggg                                         29


<210>  13
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Fig. 4 Target 1

<400>  13
atacccagtt cgactaaatg tccattaga                                         29


<210>  14
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Fig. 5 Target

<400>  14
ggccggtgtg agccgcaggt ctgtggatc                                         29


<210>  15
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Fig. 8 Target

<400>  15
tggccgtctc cagctagata ggagccacc                                         29


<210>  16
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Fig. 9 Target

<400>  16
ggcaacttca tgggtttcaa ctgcggaaa                                         29


<210>  17
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Fig. 10 Target

<400>  17
actctgttca cgccgctgct cattggctt                                         29


<210>  18
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Fig. 11 Target 1

<400>  18
ccgtgattgc gatgcgctca tggcgggct                                         29


<210>  19
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Fig. 11 Target 2

<400>  19
ctacggtgca gattcgaact gcgaggacc                                         29


<210>  20
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Fig. 12 Target

<400>  20
gggtttcaac tgcggaaact ctaagtttgg att                                    33


<210>  21
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Foxe3 Target Seq

<400>  21
gagacagccg ggcttcgcgc                                                   20


<210>  22
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Tyr (ICR) Target Seq

<400>  22
ggaaactcta agtttggatt                                                   20


<210>  23
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Agouti (1) Target Seq

<400>  23
aatggacatt tagtcgaact                                                   20


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Agouti (2) Target Seq

<400>  24
agggtttaac cacctatcga                                                   20


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Pitx3 Target Seq

<400>  25
cggtgtgagc cgcaggtctg                                                   20


<210>  26
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Tis21 Target Seq

<400>  26
ggctcctatc tagctggaga                                                   20


<210>  27
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Tyr (wild) Target Seq

<400>  27
aacttcatgg gtttcaactg                                                   20


<210>  28
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Kit Target Seq

<400>  28
ctgttcacgc cgctgctcat                                                   20


<210>  29
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: p21 (Cdkn1a) Target Seq

<400>  29
tgattgcgat gcgctcatgg                                                   20


<210>  30
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: p16/p19 (Cdkn2a) Target Seq

<400>  30
cggtgcagat tcgaactgcg                                                   20


<210>  31
<211>  131
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Tyr-rescue


<220>
<221>  misc_feature
<222>  (45)..(45)
<223>  n is inosine

<400>  31
tgttttataa taggacctgc cagtgctcag gcaacttcat gggtntcaac tgcggaaact       60

gtaagtttgg atttgggggc ccaaattgta cagagaagcg agtcttgatt agaagaaaca      120

tttttgattt g                                                           131


<210>  32
<211>  124
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Agouti-rescue


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is inosine

<220>
<221>  misc_feature
<222>  (24)..(24)
<223>  n is inosine

<220>
<221>  misc_feature
<222>  (43)..(43)
<223>  n is inosine

<220>
<221>  misc_feature
<222>  (124)..(124)
<223>  n is a, c, g, or t

<400>  32
tttattgcaa cctgcctntg cctntatatg tgttgaatat ttntagactt gatacccagt       60

gaattcgaag ggttttccca aacccctcct cagaactcag gagtatcatt aaggtactgc      120

ggtn                                                                   124


<210>  33
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M1035

<400>  33
tcctccccct atgtataccg                                                   20


<210>  34
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M1036

<400>  34
tccctgttcc tggccttag                                                    19


<210>  35
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M1037

<400>  35
cctgtgggtt gatccctatg                                                   20


<210>  36
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M1038

<400>  36
caaacactgg ctcacagatg                                                   20


<210>  37
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M1051

<400>  37
gtaatacgac tcactatagg gcccgggccg ccgccggccg ctaaccttag cccctgccag       60

tacgccgtgg aacgccggtg ggcagtggag agggcagag                              99


<210>  38
<211>  113
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M1052

<400>  38
catgaattca agccagtcta ggcgacccct gtccggagag gctgtgaatt actgccccgc       60

cctcggggat ggatccacag acctgcggct cacttgtaca gctcgtccat gcc             113


<210>  39
<211>  104
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M1053

<400>  39
gtaatacgac tcactatagg gcaagaacca gatgatgctg ggcaggagca gcccctcgaa       60

gaactatgtg atggccgtct ccagcggcag tggagagggc agag                       104


<210>  40
<211>  98
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M1054

<400>  40
catgaattct atacggtggc ctgttgtcag ggcagcatga gaacagtaga gtgccagggt       60

cgggtggctc ctatctactt gtacagctcg tccatgcc                               98


<210>  41
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M1055

<400>  41
taatacgact cactataggg agacagccgg gcttcgcgcg ttttagagct agaaatagca       60

ag                                                                      62


<210>  42
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M272

<400>  42
caggaaacag ctatgacc                                                     18


<210>  43
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M389

<400>  43
tcgccaccat ggtgagcaag ggcgag                                            26


<210>  44
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M390

<400>  44
ctctagactt tacttgtaca gctcgtccat                                        30


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M463

<400>  45
tccttctgtc cagtgcacca t                                                 21


<210>  46
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M939

<400>  46
aaaaaaagca ccgactcgg                                                    19


<210>  47
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M943

<400>  47
aggatctgtg ttcaacccat t                                                 21


<210>  48
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M944

<400>  48
acaaagaaaa ccaagcgtga c                                                 21


<210>  49
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M947

<400>  49
cttgagaaag gccacagttt c                                                 21


<210>  50
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M948

<400>  50
acgaacctct tcatctgctg t                                                 21


<210>  51
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M992

<400>  51
cctggacagc ctgttggg                                                     18


<210>  52
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M993

<400>  52
ttcagtctgg tggtgagaca g                                                 21


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: M999

<400>  53
atgggtgttg acccattgtt                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PP226

<400>  54
caagccagtc taggcgaccc                                                   20


<210>  55
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: PP227

<400>  55
catgaattct atacggtggc c                                                 21


<210>  56
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Mm HPRT FlS

<400>  56
aggtttcgag ccctgatatt cg                                                22


<210>  57
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Mm HPRTRlS

<400>  57
atgtggcaag gtcaaaaaca gt                                                22


<210>  58
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Tyr-F

<400>  58
tctctgatgg ccattttcct c                                                 21


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Tyr-R

<400>  59
aacatgggtg ttgacccatt                                                   20


<210>  60
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Kit-F


<220>
<221>  misc_feature
<222>  (18)..(18)
<223>  n is inosine

<400>  60
gagggaaatg gtttagtntg gg                                                22


<210>  61
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Kit-R

<400>  61
gggtttctgg aggagaaagg                                                   20


<210>  62
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cdknla-lw

<400>  62
cctgaagact gtgatggggt a                                                 21


<210>  63
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cdknla-rv

<400>  63
tctccgtgac gaagtcaaag t                                                 21


<210>  64
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cdkn2a-lw


<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  n is inosine

<400>  64
gccgtgatcc ctctactttn t                                                 21


<210>  65
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Cdkn2a-rv

<400>  65
tatcgcacga tgtcttgatg t                                                 21


<210>  66
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sry-F2

<400>  66
aagcgaccca tgaatgcatt catggtgtgg t                                      31


<210>  67
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Sry-R2

<400>  67
gaggtcgata cttatagttc gggtatttct ctctgtg                                37


<210>  68
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Fig. 4 Target 2

<400>  68
tccagggttt aaccacctat cgaagggtt                                         29


<210>  69
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: MmHPRT-273-S Primer

<400>  69
gtgccctctt ctggcctgcc a                                                 21


