                               SEQUENCE LISTING

<110> RUBIUS THERAPEUTICS, INC.
 
<120> LIPID NANOPARTICLE METHODS AND COMPOSITIONS FOR PRODUCING 
      ENGINEERED ERYTHROID CELLS

<130> R2081-7030WO

<140>
<141>

<150> 62/548,797
<151> 2017-08-22

<160> 11    

<170> PatentIn version 3.5

<210> 1
<211> 193
<212> PRT
<213> Homo sapiens

<400> 1
Met Gly Val His Glu Cys Pro Ala Trp Leu Trp Leu Leu Leu Ser Leu 
1               5                   10                  15      


Leu Ser Leu Pro Leu Gly Leu Pro Val Leu Gly Ala Pro Pro Arg Leu 
            20                  25                  30          


Ile Cys Asp Ser Arg Val Leu Gln Arg Tyr Leu Leu Glu Ala Lys Glu 
        35                  40                  45              


Ala Glu Asn Ile Thr Thr Gly Cys Ala Glu His Cys Ser Leu Asn Glu 
    50                  55                  60                  


Asn Ile Thr Val Pro Asp Thr Lys Val Asn Phe Tyr Ala Trp Lys Arg 
65                  70                  75                  80  


Met Glu Val Gly Gln Gln Ala Val Glu Val Trp Gln Gly Leu Ala Leu 
                85                  90                  95      


Leu Ser Glu Ala Val Leu Arg Gly Gln Ala Leu Leu Val Asn Ser Ser 
            100                 105                 110         


Gln Pro Trp Glu Pro Leu Gln Leu His Val Asp Lys Ala Val Ser Gly 
        115                 120                 125             


Leu Arg Ser Leu Thr Thr Leu Leu Arg Ala Leu Gly Ala Gln Lys Glu 
    130                 135                 140                 


Ala Ile Ser Pro Pro Asp Ala Ala Ser Ala Ala Pro Leu Arg Thr Ile 
145                 150                 155                 160 


Thr Ala Asp Thr Phe Arg Lys Leu Phe Arg Val Tyr Ser Asn Phe Leu 
                165                 170                 175     


Arg Gly Lys Leu Lys Leu Tyr Thr Gly Glu Ala Cys Arg Thr Gly Asp 
            180                 185                 190         


Arg 
    


<210> 2
<211> 166
<212> PRT
<213> Homo sapiens

<400> 2
Ala Pro Pro Arg Leu Ile Cys Asp Ser Arg Val Leu Gln Arg Tyr Leu 
1               5                   10                  15      


Leu Glu Ala Lys Glu Ala Glu Asn Ile Thr Thr Gly Cys Ala Glu His 
            20                  25                  30          


Cys Ser Leu Asn Glu Asn Ile Thr Val Pro Asp Thr Lys Val Asn Phe 
        35                  40                  45              


Tyr Ala Trp Lys Arg Met Glu Val Gly Gln Gln Ala Val Glu Val Trp 
    50                  55                  60                  


Gln Gly Leu Ala Leu Leu Ser Glu Ala Val Leu Arg Gly Gln Ala Leu 
65                  70                  75                  80  


Leu Val Asn Ser Ser Gln Pro Trp Glu Pro Leu Gln Leu His Val Asp 
                85                  90                  95      


Lys Ala Val Ser Gly Leu Arg Ser Leu Thr Thr Leu Leu Arg Ala Leu 
            100                 105                 110         


Gly Ala Gln Lys Glu Ala Ile Ser Pro Pro Asp Ala Ala Ser Ala Ala 
        115                 120                 125             


Pro Leu Arg Thr Ile Thr Ala Asp Thr Phe Arg Lys Leu Phe Arg Val 
    130                 135                 140                 


Tyr Ser Asn Phe Leu Arg Gly Lys Leu Lys Leu Tyr Thr Gly Glu Ala 
145                 150                 155                 160 


Cys Arg Thr Gly Asp Arg 
                165     


<210> 3
<211> 192
<212> PRT
<213> Rattus sp.

<400> 3
Met Gly Val Pro Glu Arg Pro Thr Leu Leu Leu Leu Leu Ser Leu Leu 
1               5                   10                  15      


Leu Ile Pro Leu Gly Leu Pro Val Leu Cys Ala Pro Pro Arg Leu Ile 
            20                  25                  30          


Cys Asp Ser Arg Val Leu Glu Arg Tyr Ile Leu Glu Ala Lys Glu Ala 
        35                  40                  45              


Glu Asn Val Thr Met Gly Cys Ala Glu Gly Pro Arg Leu Ser Glu Asn 
    50                  55                  60                  


Ile Thr Val Pro Asp Thr Lys Val Asn Phe Tyr Ala Trp Lys Arg Met 
65                  70                  75                  80  


Lys Val Glu Glu Gln Ala Val Glu Val Trp Gln Gly Leu Ser Leu Leu 
                85                  90                  95      


Ser Glu Ala Ile Leu Gln Ala Gln Ala Leu Gln Ala Asn Ser Ser Gln 
            100                 105                 110         


Pro Pro Glu Ser Leu Gln Leu His Ile Asp Lys Ala Ile Ser Gly Leu 
        115                 120                 125             


Arg Ser Leu Thr Ser Leu Leu Arg Val Leu Gly Ala Gln Lys Glu Leu 
    130                 135                 140                 


Met Ser Pro Pro Asp Ala Thr Gln Ala Ala Pro Leu Arg Thr Leu Thr 
145                 150                 155                 160 


Ala Asp Thr Phe Cys Lys Leu Phe Arg Val Tyr Ser Asn Phe Leu Arg 
                165                 170                 175     


Gly Lys Leu Lys Leu Tyr Thr Gly Glu Ala Cys Arg Arg Gly Asp Arg 
            180                 185                 190         


<210> 4
<211> 192
<212> PRT
<213> Mus sp.

<400> 4
Met Gly Val Pro Glu Arg Pro Thr Leu Leu Leu Leu Leu Ser Leu Leu 
1               5                   10                  15      


Leu Ile Pro Leu Gly Leu Pro Val Leu Cys Ala Pro Pro Arg Leu Ile 
            20                  25                  30          


Cys Asp Ser Arg Val Leu Glu Arg Tyr Ile Leu Glu Ala Lys Glu Ala 
        35                  40                  45              


Glu Asn Val Thr Met Gly Cys Ala Glu Gly Pro Arg Leu Ser Glu Asn 
    50                  55                  60                  


Ile Thr Val Pro Asp Thr Lys Val Asn Phe Tyr Ala Trp Lys Arg Met 
65                  70                  75                  80  


Glu Val Glu Glu Gln Ala Ile Glu Val Trp Gln Gly Leu Ser Leu Leu 
                85                  90                  95      


Ser Glu Ala Ile Leu Gln Ala Gln Ala Leu Leu Ala Asn Ser Ser Gln 
            100                 105                 110         


Pro Pro Glu Thr Leu Gln Leu His Ile Asp Lys Ala Ile Ser Gly Leu 
        115                 120                 125             


Arg Ser Leu Thr Ser Leu Leu Arg Val Leu Gly Ala Gln Lys Glu Leu 
    130                 135                 140                 


Met Ser Pro Pro Asp Thr Thr Pro Pro Ala Pro Leu Arg Thr Leu Thr 
145                 150                 155                 160 


Val Asp Thr Phe Cys Lys Leu Phe Arg Val Tyr Ala Asn Phe Leu Arg 
                165                 170                 175     


Gly Lys Leu Lys Leu Tyr Thr Gly Glu Val Cys Arg Arg Gly Asp Arg 
            180                 185                 190         


<210> 5
<211> 6
<212> PRT
<213> Unknown

<220>
<221> source
<223> /note="Description of Unknown: 
      Internalization signal sequence"


<220>
<221> MOD_RES
<222> (2)..(2)
<223> Any amino acid

<220>
<221> MOD_RES
<222> (5)..(5)
<223> Any amino acid

<400> 5
Phe Xaa Asn Pro Xaa Tyr 
1               5       


<210> 6
<211> 6
<212> PRT
<213> Unknown

<220>
<221> source
<223> /note="Description of Unknown: 
      Dileucine-dependent signal-sequence motif"


<220>
<221> MOD_RES
<222> (3)..(3)
<223> Any amino acid residue with a hydrophobic side chain

<400> 6
Asp Lys Xaa Thr Leu Leu 
1               5       


<210> 7
<211> 4
<212> PRT
<213> Unknown

<220>
<221> source
<223> /note="Description of Unknown: 
      Dileucine-dependent signal-sequence motif"

<400> 7
Leu Leu His Val 
1               


<210> 8
<211> 5
<212> PRT
<213> Unknown

<220>
<221> source
<223> /note="Description of Unknown: 
      Dileucine-dependent signal-sequence motif"

<400> 8
His Leu Leu Pro Met 
1               5   


<210> 9
<211> 7
<212> PRT
<213> Unknown

<220>
<221> source
<223> /note="Description of Unknown: 
      Targeting agent sequence"

<400> 9
His Ala Ile Tyr Pro Arg His 
1               5           


<210> 10
<211> 12
<212> PRT
<213> Unknown

<220>
<221> source
<223> /note="Description of Unknown: 
      Targeting agent sequence"

<400> 10
Thr His Arg Pro Pro Met Trp Ser Pro Val Trp Pro 
1               5                   10          


<210> 11
<211> 698
<212> PRT
<213> Homo sapiens

<400> 11
Met Arg Leu Ala Val Gly Ala Leu Leu Val Cys Ala Val Leu Gly Leu 
1               5                   10                  15      


Cys Leu Ala Val Pro Asp Lys Thr Val Arg Trp Cys Ala Val Ser Glu 
            20                  25                  30          


His Glu Ala Thr Lys Cys Gln Ser Phe Arg Asp His Met Lys Ser Val 
        35                  40                  45              


Ile Pro Ser Asp Gly Pro Ser Val Ala Cys Val Lys Lys Ala Ser Tyr 
    50                  55                  60                  


Leu Asp Cys Ile Arg Ala Ile Ala Ala Asn Glu Ala Asp Ala Val Thr 
65                  70                  75                  80  


Leu Asp Ala Gly Leu Val Tyr Asp Ala Tyr Leu Ala Pro Asn Asn Leu 
                85                  90                  95      


Lys Pro Val Val Ala Glu Phe Tyr Gly Ser Lys Glu Asp Pro Gln Thr 
            100                 105                 110         


Phe Tyr Tyr Ala Val Ala Val Val Lys Lys Asp Ser Gly Phe Gln Met 
        115                 120                 125             


Asn Gln Leu Arg Gly Lys Lys Ser Cys His Thr Gly Leu Gly Arg Ser 
    130                 135                 140                 


Ala Gly Trp Asn Ile Pro Ile Gly Leu Leu Tyr Cys Asp Leu Pro Glu 
145                 150                 155                 160 


Pro Arg Lys Pro Leu Glu Lys Ala Val Ala Asn Phe Phe Ser Gly Ser 
                165                 170                 175     


Cys Ala Pro Cys Ala Asp Gly Thr Asp Phe Pro Gln Leu Cys Gln Leu 
            180                 185                 190         


Cys Pro Gly Cys Gly Cys Ser Thr Leu Asn Gln Tyr Phe Gly Tyr Ser 
        195                 200                 205             


Gly Ala Phe Lys Cys Leu Lys Asp Gly Ala Gly Asp Val Ala Phe Val 
    210                 215                 220                 


Lys His Ser Thr Ile Phe Glu Asn Leu Ala Asn Lys Ala Asp Arg Asp 
225                 230                 235                 240 


Gln Tyr Glu Leu Leu Cys Leu Asp Asn Thr Arg Lys Pro Val Asp Glu 
                245                 250                 255     


Tyr Lys Asp Cys His Leu Ala Gln Val Pro Ser His Thr Val Val Ala 
            260                 265                 270         


Arg Ser Met Gly Gly Lys Glu Asp Leu Ile Trp Glu Leu Leu Asn Gln 
        275                 280                 285             


Ala Gln Glu His Phe Gly Lys Asp Lys Ser Lys Glu Phe Gln Leu Phe 
    290                 295                 300                 


Ser Ser Pro His Gly Lys Asp Leu Leu Phe Lys Asp Ser Ala His Gly 
305                 310                 315                 320 


Phe Leu Lys Val Pro Pro Arg Met Asp Ala Lys Met Tyr Leu Gly Tyr 
                325                 330                 335     


Glu Tyr Val Thr Ala Ile Arg Asn Leu Arg Glu Gly Thr Cys Pro Glu 
            340                 345                 350         


Ala Pro Thr Asp Glu Cys Lys Pro Val Lys Trp Cys Ala Leu Ser His 
        355                 360                 365             


His Glu Arg Leu Lys Cys Asp Glu Trp Ser Val Asn Ser Val Gly Lys 
    370                 375                 380                 


Ile Glu Cys Val Ser Ala Glu Thr Thr Glu Asp Cys Ile Ala Lys Ile 
385                 390                 395                 400 


Met Asn Gly Glu Ala Asp Ala Met Ser Leu Asp Gly Gly Phe Val Tyr 
                405                 410                 415     


Ile Ala Gly Lys Cys Gly Leu Val Pro Val Leu Ala Glu Asn Tyr Asn 
            420                 425                 430         


Lys Ser Asp Asn Cys Glu Asp Thr Pro Glu Ala Gly Tyr Phe Ala Ile 
        435                 440                 445             


Ala Val Val Lys Lys Ser Ala Ser Asp Leu Thr Trp Asp Asn Leu Lys 
    450                 455                 460                 


Gly Lys Lys Ser Cys His Thr Ala Val Gly Arg Thr Ala Gly Trp Asn 
465                 470                 475                 480 


Ile Pro Met Gly Leu Leu Tyr Asn Lys Ile Asn His Cys Arg Phe Asp 
                485                 490                 495     


Glu Phe Phe Ser Glu Gly Cys Ala Pro Gly Ser Lys Lys Asp Ser Ser 
            500                 505                 510         


Leu Cys Lys Leu Cys Met Gly Ser Gly Leu Asn Leu Cys Glu Pro Asn 
        515                 520                 525             


Asn Lys Glu Gly Tyr Tyr Gly Tyr Thr Gly Ala Phe Arg Cys Leu Val 
    530                 535                 540                 


Glu Lys Gly Asp Val Ala Phe Val Lys His Gln Thr Val Pro Gln Asn 
545                 550                 555                 560 


Thr Gly Gly Lys Asn Pro Asp Pro Trp Ala Lys Asn Leu Asn Glu Lys 
                565                 570                 575     


Asp Tyr Glu Leu Leu Cys Leu Asp Gly Thr Arg Lys Pro Val Glu Glu 
            580                 585                 590         


Tyr Ala Asn Cys His Leu Ala Arg Ala Pro Asn His Ala Val Val Thr 
        595                 600                 605             


Arg Lys Asp Lys Glu Ala Cys Val His Lys Ile Leu Arg Gln Gln Gln 
    610                 615                 620                 


His Leu Phe Gly Ser Asn Val Thr Asp Cys Ser Gly Asn Phe Cys Leu 
625                 630                 635                 640 


Phe Arg Ser Glu Thr Lys Asp Leu Leu Phe Arg Asp Asp Thr Val Cys 
                645                 650                 655     


Leu Ala Lys Leu His Asp Arg Asn Thr Tyr Glu Lys Tyr Leu Gly Glu 
            660                 665                 670         


Glu Tyr Val Lys Ala Val Gly Asn Leu Arg Lys Cys Ser Thr Ser Ser 
        675                 680                 685             


Leu Leu Glu Ala Cys Thr Phe Arg Arg Pro 
    690                 695             



