                         SEQUENCE LISTING

<110>  University of Tennessee Research Foundation
 
<120>  Staphylococcus pseudintermedius Virulence Factor Compositions

<130>  1101-016 PCT

<150>  US 62/543676
<151>  2017-08-10

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  1272
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  S. pseudintermedius protein A synthetic gene optimized for 
       expression in E.coli

<400>  1
attggcggaa ggccgtcaag gccgcatgag ctcgcatgag gatccaagct ttgccgaaga       60

aggtgataat aacgccgaag ccaaaaaaaa cgcatttagc gaagttgtga aactgccgaa      120

tctgagcgaa gaacagcgta atggttttat tcagagcctg aaagcagcac cgagcaccag      180

ccaggatgtt ctgaatgaag caaaaaaact gaacgatagc caagaaggta gccagcctgc      240

accggattat agtgatgaaa aaaagaacgc cttctatgaa attctgcatc tgccgaattt      300

gacagaggaa caacgcaatg gttatatcca gtcattaaaa gccgcaccgt cagttagcgc      360

aaatattctg gttgaagcga aaaacatgaa cgttaatcag accccgacac aaccggcacc      420

gagctttgat gaagcgaaga aaaatgcctt ttacgagatt gttaacctgc cgaacttaac      480

cgaagaacaa agaaacggct ttatccaaag tcttaaagca gctccgtctg tgagcaaaga      540

tatcctggtg gaagccaaga aactgaatga ttctcaggca aaaccggatt acaacgaggc      600

aaaaaagaat gcattttatg agatccttca ccttccgaac ctgactgagg aacagagaaa      660

tggattcatt cagtctctga aggctgcacc gagtgttagc agcgatattc tggcagaggc      720

caaaaaactt aatgacagcc aggcaccgaa agaggataac aacgttaaag ataataacag      780

cggtgagaac aaagccgaag ataaaggcaa taaagaaaac aaggcagagg acaaaggtag      840

caaagaagat aaggccgagg ataagggttc aaaagaggac aaggcggaag ataagggctc      900

taaagaagat aaagccaaag acaaggataa caaagaaggt aaagcagccg ataaaggtat      960

ggacaaagca aaagatgcaa tgcatgttgt tcagcctggt gaaaccgttg aaaaaattgc     1020

caaagcaaat aacaccaccg ttgagcagat tgcgaaagat aatcatcttg aggacaagaa     1080

catgattctg cctggtcaga aactggttgt ggataatcag aaagcaatga aggattccaa     1140

agaagccaaa gccaatcacg aaatgaaagc actgccggaa acaggtgaag aaaatgatat     1200

ggcactgttc ggtgcggccg ctcatgcggt accctgggcc tcatgggcct tccgctcacg     1260

ccttccgctc ac                                                         1272


<210>  2
<211>  393
<212>  PRT
<213>  artificial sequence

<220>
<223>  S. pseudintermedius attenuated Protein A

<400>  2

Pro Ser Phe Ala Glu Glu Gly Asp Asn Asn Ala Glu Ala Lys Lys Asn 
1               5                   10                  15      


Ala Phe Ser Glu Val Val Lys Leu Pro Asn Leu Ser Glu Glu Gln Arg 
            20                  25                  30          


Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala Pro Ser Thr Ser Gln Asp 
        35                  40                  45              


Val Leu Asn Glu Ala Lys Lys Leu Asn Asp Ser Gln Glu Gly Ser Gln 
    50                  55                  60                  


Pro Ala Pro Asp Tyr Ser Asp Glu Lys Lys Asn Ala Phe Tyr Glu Ile 
65                  70                  75                  80  


Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Gly Tyr Ile Gln 
                85                  90                  95      


Ser Leu Lys Ala Ala Pro Ser Val Ser Ala Asn Ile Leu Val Glu Ala 
            100                 105                 110         


Lys Asn Met Asn Val Asn Gln Thr Pro Thr Gln Pro Ala Pro Ser Phe 
        115                 120                 125             


Asp Glu Ala Lys Lys Asn Ala Phe Tyr Glu Ile Val Asn Leu Pro Asn 
    130                 135                 140                 


Leu Thr Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Ala Ala 
145                 150                 155                 160 


Pro Ser Val Ser Lys Asp Ile Leu Val Glu Ala Lys Lys Leu Asn Asp 
                165                 170                 175     


Ser Gln Ala Lys Pro Asp Tyr Asn Glu Ala Lys Lys Asn Ala Phe Tyr 
            180                 185                 190         


Glu Ile Leu His Leu Pro Asn Leu Thr Glu Glu Gln Arg Asn Gly Phe 
        195                 200                 205             


Ile Gln Ser Leu Lys Ala Ala Pro Ser Val Ser Ser Asp Ile Leu Ala 
    210                 215                 220                 


Glu Ala Lys Lys Leu Asn Asp Ser Gln Ala Pro Lys Glu Asp Asn Asn 
225                 230                 235                 240 


Val Lys Asp Asn Asn Ser Gly Glu Asn Lys Ala Glu Asp Lys Gly Asn 
                245                 250                 255     


Lys Glu Asn Lys Ala Glu Asp Lys Gly Ser Lys Glu Asp Lys Ala Glu 
            260                 265                 270         


Asp Lys Gly Ser Lys Glu Asp Lys Ala Glu Asp Lys Gly Ser Lys Glu 
        275                 280                 285             


Asp Lys Ala Lys Asp Lys Asp Asn Lys Glu Gly Lys Ala Ala Asp Lys 
    290                 295                 300                 


Gly Met Asp Lys Ala Lys Asp Ala Met His Val Val Gln Pro Gly Glu 
305                 310                 315                 320 


Thr Val Glu Lys Ile Ala Lys Ala Asn Asn Thr Thr Val Glu Gln Ile 
                325                 330                 335     


Ala Lys Asp Asn His Leu Glu Asp Lys Asn Met Ile Leu Pro Gly Gln 
            340                 345                 350         


Lys Leu Val Val Asp Asn Gln Lys Ala Met Lys Asp Ser Lys Glu Ala 
        355                 360                 365             


Lys Ala Asn His Glu Met Lys Ala Leu Pro Glu Thr Gly Glu Glu Asn 
    370                 375                 380                 


Asp Met Ala Leu Phe Gly Ala Ala Ala 
385                 390             


<210>  3
<211>  1037
<212>  DNA
<213>  artificial sequence

<220>
<223>  S. pseduintermedius Leukotoxin S synthetic gene optimized for 
       expression in E.coli

<400>  3
cgaattggcg gaaggccgtc aaggccacgt gtcttgtcca gagctcggat ccaatggtga       60

aaaacaaact gctggcagca accctgagca ttagcctggt tctgccgctg attaccccgt      120

atagcgaaga agcaaaagca gcaaatacca ttgaagaaat tggtgaaggt gcccagatta      180

tcaaacgtac cgaagatgtt agcagccgta aatggggtgt ttttcagaac atccagttcg      240

attttgtgaa agacccgaaa tataataagg acgccctgat cattaaaatg cagggcttta      300

tcaaaagccg taccagcttt accgatgtga aaggtaaagg ttatgaaagc accaaacgta      360

tgctgtggcc gtttcagtat aacattgcac tgaaaaccaa tgatccgaat gtgagcctga      420

tcaattatct gcctgcaaac aaaattgaga gcatcgatgt tagtcagacc ctgggttata      480

atgttggtgg taattttcag agcgcaccgc tgttaggtgg taaaggtgca tttaactaca      540

gcaagaaaat cagctacacc cagaaaaact atattagcga agttgcacag cagaacagca      600

aaaacattcg ttgggaagtt aaagccaaca gctttaatac cgaaaatggt caggttagcg      660

catatgatcg tcacctgttt gttcgtagcc cgattggtcc gaatgcacgt gatttttttg      720

ttccgaatga tgaactgcct ccgctgattc agagcggttt taatccggca tttattgcaa      780

ccgttagcca cgaaaaagat aaaggtgata ccagcgaatt tgaaattgcc tatggtcgca      840

atctggatat tacctatgca accttttttc cgcgtaccgg tatttttgca gaacgtcgtc      900

ataatgcact gatgaatcgt aatctggtga ccaaatatga ggtgaactgg aaaacccatg      960

agatcaaagt gaaaggccac aatgcggccg cggtacctgg agcacaagac tggcctcatg     1020

ggccttccgc tcactgc                                                    1037


<210>  4
<211>  424
<212>  PRT
<213>  artificial sequence

<220>
<223>  S. pseuduintermedius attenuated  Leukotoxin S

<400>  4

Ala Thr Thr Thr Thr Thr Gly Cys Ala Gly Ala Ala Cys Gly Thr Cys 
1               5                   10                  15      


Gly Thr Cys Ala Thr Ala Ala Thr Gly Cys Ala Cys Thr Gly Ala Thr 
            20                  25                  30          


Gly Ala Ala Thr Cys Gly Thr Ala Ala Thr Cys Thr Gly Gly Thr Gly 
        35                  40                  45              


Ala Cys Cys Ala Ala Ala Thr Ala Thr Gly Ala Gly Gly Thr Gly Ala 
    50                  55                  60                  


Ala Cys Thr Gly Gly Ala Ala Ala Ala Cys Cys Cys Ala Thr Gly Ala 
65                  70                  75                  80  


Gly Ala Thr Cys Ala Ala Ala Gly Thr Gly Ala Ala Ala Gly Gly Cys 
                85                  90                  95      


Cys Ala Cys Ala Ala Thr Gly Cys Gly Gly Cys Cys Gly Cys Gly Ser 
            100                 105                 110         


Met Val Lys Asn Lys Leu Leu Ala Ala Thr Leu Ser Ile Ser Leu Val 
        115                 120                 125             


Leu Pro Leu Ile Thr Pro Tyr Ser Glu Glu Ala Lys Ala Ala Asn Thr 
    130                 135                 140                 


Ile Glu Glu Ile Gly Glu Gly Ala Gln Ile Ile Lys Arg Thr Glu Asp 
145                 150                 155                 160 


Val Ser Ser Arg Lys Trp Gly Val Phe Gln Asn Ile Gln Phe Asp Phe 
                165                 170                 175     


Val Lys Asp Pro Lys Tyr Asn Lys Asp Ala Leu Ile Ile Lys Met Gln 
            180                 185                 190         


Gly Phe Ile Lys Ser Arg Thr Ser Phe Thr Asp Val Lys Gly Lys Gly 
        195                 200                 205             


Tyr Glu Ser Thr Lys Arg Met Leu Trp Pro Phe Gln Tyr Asn Ile Ala 
    210                 215                 220                 


Leu Lys Thr Asn Asp Pro Asn Val Ser Leu Ile Asn Tyr Leu Pro Ala 
225                 230                 235                 240 


Asn Lys Ile Glu Ser Ile Asp Val Ser Gln Thr Leu Gly Tyr Asn Val 
                245                 250                 255     


Gly Gly Asn Phe Gln Ser Ala Pro Leu Leu Gly Gly Lys Gly Ala Phe 
            260                 265                 270         


Asn Tyr Ser Lys Lys Ile Ser Tyr Thr Gln Lys Asn Tyr Ile Ser Glu 
        275                 280                 285             


Val Ala Gln Gln Asn Ser Lys Asn Ile Arg Trp Glu Val Lys Ala Asn 
    290                 295                 300                 


Ser Phe Asn Thr Glu Asn Gly Gln Val Ser Ala Tyr Asp Arg His Leu 
305                 310                 315                 320 


Phe Val Arg Ser Pro Ile Gly Pro Asn Ala Arg Asp Phe Phe Val Pro 
                325                 330                 335     


Asn Asp Glu Leu Pro Pro Leu Ile Gln Ser Gly Phe Asn Pro Ala Phe 
            340                 345                 350         


Ile Ala Thr Val Ser His Glu Lys Asp Lys Gly Asp Thr Ser Glu Phe 
        355                 360                 365             


Glu Ile Ala Tyr Gly Arg Asn Leu Asp Ile Thr Tyr Ala Thr Phe Phe 
    370                 375                 380                 


Pro Arg Thr Gly Ile Phe Ala Glu Arg Arg His Asn Ala Leu Met Asn 
385                 390                 395                 400 


Arg Asn Leu Val Thr Lys Tyr Glu Val Asn Trp Lys Thr His Glu Ile 
                405                 410                 415     


Lys Val Lys Gly His Asn Ala Ala 
            420                 


<210>  5
<211>  1470
<212>  DNA
<213>  artificial sequence

<220>
<223>  S. pseuduintermedius Nucleotidase ADsA synthetic gene optimized 
       for expression in E.coli

<400>  5
atgctgcgtc tgagcgcaaa aaaagcacag ccggaagcaa ccagcattga tcgtgcaagc       60

catgaaaccg cagcaaccac acataccatt ctgcatacca atgcaattgc cggtcgtatg      120

gttgaagaaa aagatcgtgt tttaggtatg gccaaactga aaaccctgaa agaacagcag      180

aacccggatc tgctggttga tgccggtgat gcatttcagg gtctgccgct gagcaatcag      240

agcaaaggtg aagaaatggc aaaagcaatg aatgccgttg gttatgatgc aatgaccgca      300

ggtaatgcag aatttgcctt tggctatgat cagctgaaaa aactggaagg catgctgaat      360

tttccgattg ttagcagcaa cgtgtataaa gatggtaaac tggcatttaa accgagcgtg      420

gtgattcaga aaaatggtgt tcgttatggt gtgattggtg ttaccacacc ggaaaccaaa      480

acaaaaacca gtccgaaagg tattgttggt gtgacctttg cagatccgct gaccagcgtt      540

acccgtgaaa tggatcgtct gaatggtcag gttgatgttt ttattgttct gagccatctg      600

ggtattgatc cgaccaccaa agaagcatgg cgaggcgatt atctgacccg tcagctgagc      660

cagaataaac agtatcatca tccgatcttt gtgatcgatg gtcatagcca taccgttatt      720

gaacatggcc agaaatttga tcaggatgtt ctggcacaga ccggcaccgc actggcaaat      780

gtgggtaaac tgaccttcaa acagagcggt cagcagttta gcaatgccga agcaagcctg      840

ctgaatgtta aaaatctggc aaaactgcag ccggatgcag cagttaaagc acaggttgat      900

aaagcaaatg aagcctttct gaaagcgacc agcgaagtta ttattccgaa taacaccgtt      960

gattttcagg gcgaacgtga tgatgttcgt acccatgaaa caaatctggg taatgcaatt     1020

accgatgcaa tggaagcata tggtcagaaa ggttttagcc gtccgagcga ttttgcagtt     1080

accaatagcg gtggtattcg tgccagcatt gcaaaaggta aagttaccct gaatgatgtg     1140

attaccgttc tgccgtttgg taataccatt gcacagatta gcgttaaagg ttccgatgtt     1200

tggaaagcct ttgaacatag cctgagcgca ccgaccatga ccacaggtgg tgaaacccag     1260

ctgagcgcca atggtggtct gctgcaggtt agcaaaagca ttcaggttta tttcgacatg     1320

aataaagcac cgggtgaacg tattaatgcc attcgtgttc tgaacaaaca gacaggccag     1380

tttgaagatc tggatatgag ccgtacctat gcagttgcca tgaatgattt taccgcaagc     1440

ggtggtgcag cagcacatgc aaatgcatat                                      1470


<210>  6
<211>  490
<212>  PRT
<213>  artificial sequence

<220>
<223>  S. pseduintermedius attenuated  Nucleotidase ADsA

<400>  6

Met Leu Arg Leu Ser Ala Lys Lys Ala Gln Pro Glu Ala Thr Ser Ile 
1               5                   10                  15      


Asp Arg Ala Ser His Glu Thr Ala Ala Thr Thr His Thr Ile Leu His 
            20                  25                  30          


Thr Asn Ala Ile Ala Gly Arg Met Val Glu Glu Lys Asp Arg Val Leu 
        35                  40                  45              


Gly Met Ala Lys Leu Lys Thr Leu Lys Glu Gln Gln Asn Pro Asp Leu 
    50                  55                  60                  


Leu Val Asp Ala Gly Asp Ala Phe Gln Gly Leu Pro Leu Ser Asn Gln 
65                  70                  75                  80  


Ser Lys Gly Glu Glu Met Ala Lys Ala Met Asn Ala Val Gly Tyr Asp 
                85                  90                  95      


Ala Met Thr Ala Gly Asn Ala Glu Phe Ala Phe Gly Tyr Asp Gln Leu 
            100                 105                 110         


Lys Lys Leu Glu Gly Met Leu Asn Phe Pro Ile Val Ser Ser Asn Val 
        115                 120                 125             


Tyr Lys Asp Gly Lys Leu Ala Phe Lys Pro Ser Val Val Ile Gln Lys 
    130                 135                 140                 


Asn Gly Val Arg Tyr Gly Val Ile Gly Val Thr Thr Pro Glu Thr Lys 
145                 150                 155                 160 


Thr Lys Thr Ser Pro Lys Gly Ile Val Gly Val Thr Phe Ala Asp Pro 
                165                 170                 175     


Leu Thr Ser Val Thr Arg Glu Met Asp Arg Leu Asn Gly Gln Val Asp 
            180                 185                 190         


Val Phe Ile Val Leu Ser His Leu Gly Ile Asp Pro Thr Thr Lys Glu 
        195                 200                 205             


Ala Trp Arg Gly Asp Tyr Leu Thr Arg Gln Leu Ser Gln Asn Lys Gln 
    210                 215                 220                 


Tyr His His Pro Ile Phe Val Ile Asp Gly His Ser His Thr Val Ile 
225                 230                 235                 240 


Glu His Gly Gln Lys Phe Asp Gln Asp Val Leu Ala Gln Thr Gly Thr 
                245                 250                 255     


Ala Leu Ala Asn Val Gly Lys Leu Thr Phe Lys Gln Ser Gly Gln Gln 
            260                 265                 270         


Phe Ser Asn Ala Glu Ala Ser Leu Leu Asn Val Lys Asn Leu Ala Lys 
        275                 280                 285             


Leu Gln Pro Asp Ala Ala Val Lys Ala Gln Val Asp Lys Ala Asn Glu 
    290                 295                 300                 


Ala Phe Leu Lys Ala Thr Ser Glu Val Ile Ile Pro Asn Asn Thr Val 
305                 310                 315                 320 


Asp Phe Gln Gly Glu Arg Asp Asp Val Arg Thr His Glu Thr Asn Leu 
                325                 330                 335     


Gly Asn Ala Ile Thr Asp Ala Met Glu Ala Tyr Gly Gln Lys Gly Phe 
            340                 345                 350         


Ser Arg Pro Ser Asp Phe Ala Val Thr Asn Ser Gly Gly Ile Arg Ala 
        355                 360                 365             


Ser Ile Ala Lys Gly Lys Val Thr Leu Asn Asp Val Ile Thr Val Leu 
    370                 375                 380                 


Pro Phe Gly Asn Thr Ile Ala Gln Ile Ser Val Lys Gly Ser Asp Val 
385                 390                 395                 400 


Trp Lys Ala Phe Glu His Ser Leu Ser Ala Pro Thr Met Thr Thr Gly 
                405                 410                 415     


Gly Glu Thr Gln Leu Ser Ala Asn Gly Gly Leu Leu Gln Val Ser Lys 
            420                 425                 430         


Ser Ile Gln Val Tyr Phe Asp Met Asn Lys Ala Pro Gly Glu Arg Ile 
        435                 440                 445             


Asn Ala Ile Arg Val Leu Asn Lys Gln Thr Gly Gln Phe Glu Asp Leu 
    450                 455                 460                 


Asp Met Ser Arg Thr Tyr Ala Val Ala Met Asn Asp Phe Thr Ala Ser 
465                 470                 475                 480 


Gly Gly Ala Ala Ala His Ala Asn Ala Tyr 
                485                 490 


<210>  7
<211>  1497
<212>  DNA
<213>  artificial sequence

<220>
<223>  S. pseudintermedius coagulase synthetic gene optimized for 
       expression in E.coli

<400>  7
ggatccaatg atgaaaaaaa agctgctggt tctgagcgca agcgcagttc tggcaagcca       60

ttttctggtt ggtggtgaag ccagcgcagc agcaagcggt gaaaaaaatc cgtataccag      120

caaagcactg agcatcatgg aaaaaagcag caccagcagc gttaccaccg aacagtacaa      180

aaaaagcgca gaaaaagcaa tggcacgtct gaccgcaggc ggttatgaag catatgacga      240

accggcagca gcagccgttg ttaaagcata taaacagcgt tttgcagccg aaatgaatgc      300

catgaatcag tttgcccaag aagagaaagc caaagaaaaa aagaatggtg ccattagcac      360

cgatgcaatt ggtctgaccc atcagcgtta tgataccgca tatgcaagtg ccgaagaaaa      420

cgcaaaagat tttgaacgcg agatcaaaaa actgaacgaa cagcatagcg atctgaaaac      480

ctttaatcgc caagaacaga atgatgcaga tcagaaagca aacgatctgg aaaatcaggc      540

actgatgctg ggccagacct ttatctatca gggtgatgcc attttcaacc tgtataacaa      600

actggatatg agcattggca acctggatta tgaacgggaa gagaaaaaag ccattaacaa      660

acgcatgctg gacaagaaag ttgaggacct ggaaaccatt atcgatgaat tttttgcagc      720

gattgcactg aaacgtccga aagaagcacc ggttctgacc cgtgaaaatg aaaaagatag      780

cgcagttaaa gcaaaactgc gtcaggatgc acaggatgcc aaaaatgatg caagcctgcg      840

tcatccgcgt gttctgaaaa agaaaagtac cgatgcaccg agccagccga gcgatcaaga      900

agttgcacag ccgtcagtta atagcattga aattaaagca ccgcaggcag caaccccgaa      960

aaaaccggtt cataccgaaa ccattgttca taccccgatg accgcagttc cgcagaccaa     1020

acatgaagca acctttgaag tgccgcaggg tcgtaatacc cagaccagcg caaccgcaaa     1080

tagcgaacag gcaaccgaaa ccgttgatgt tccgaaagca aatggtcagg ttcagagtga     1140

acaggttacc acaccgggtg ttcagcagac ccatagcatg gcacagcaga gcgaaacacc     1200

gcagccggtt gcagaagaaa ccgaaattat caccgaagca catgttgccg atattgatga     1260

aagcaccacc tttcagaaaa gcggttatct ggcaggcgca agcgaaagcg ataccagcgg     1320

tgcaaccgaa gcagaaaaac gtgcaattcg tcgtgcacat gttcgtgaag cagaagcact     1380

ggcagcacag gcagcagaaa cccatcgtgc ccaggatgca atggcagccc agcagaaagt     1440

tgcaaccctg agcaaagccc atcagaaacg ttttaacaag atgattaacg cggccgc        1497


<210>  8
<211>  502
<212>  PRT
<213>  artificial sequence

<220>
<223>  S. pseudintermedius  attenuated coagulase

<400>  8

Met Lys Lys Lys Leu Leu Val Leu Ser Ala Ser Ala Val Leu Ala Ser 
1               5                   10                  15      


His Phe Leu Val Gly Gly Glu Ala Ser Ala Ile Val Ser Gly Glu Glu 
            20                  25                  30          


Asn Pro Tyr Lys Ser Lys Ala Leu Ser Ile Thr Glu Lys Ser Ser Thr 
        35                  40                  45              


Ser Ser Tyr Thr Ser Glu Gln Tyr Lys Lys Ser Leu Glu Asp Leu Ile 
    50                  55                  60                  


Tyr Lys Leu Thr Ile Ser Gly Tyr Glu Lys Tyr Asp Glu Pro Glu Tyr 
65                  70                  75                  80  


Ala Ala Ala Val Lys Lys Tyr Gln Gln Arg Phe Met Ala Glu Met Glu 
                85                  90                  95      


Ala Met Asn Gln Phe Leu Gln Gly Glu Lys Val Lys Glu Arg Lys Arg 
            100                 105                 110         


Lys Asn Ser Gln Pro Ile Pro Asp Asp Ile Ile Gly Leu Thr Tyr Gln 
        115                 120                 125             


Arg Tyr Lys Ala Val Tyr Asp Ala Leu Glu Glu Asn Lys Lys Asp Phe 
    130                 135                 140                 


Asp Arg Glu Ile Ala Glu Leu Asn Asp Lys His Pro Glu Leu Lys Thr 
145                 150                 155                 160 


Phe Asp Ser Lys His Gln Ser Glu Ala Asp Gln Lys Leu Asn Asp Leu 
                165                 170                 175     


Glu Asn Gln Val Leu Met Leu Gly Lys Thr Phe Ile Arg Lys Asp Glu 
            180                 185                 190         


Ala Arg Ala Ser Leu Tyr Gln Lys Leu Asp Leu Ser Ile Gly Tyr Thr 
        195                 200                 205             


Asp Asn Glu Arg Lys Glu Lys Lys Ala Ile Asn Gln Arg Met Leu Asp 
    210                 215                 220                 


Arg Lys Ile Glu Asp Leu Glu Thr Ile Ile Asp Glu Phe Phe Glu Glu 
225                 230                 235                 240 


Ala Asp Leu Ala Arg Pro Lys Glu Ile Pro Val Leu Thr Ala Glu Asn 
                245                 250                 255     


Glu Tyr Asp Asn Ala Val Lys Asn Lys Leu Arg Glu Asp Ala Asn Lys 
            260                 265                 270         


Ala Lys Ala Asp Glu Lys Leu Arg His Pro Arg Ala Leu Lys Gln Gln 
        275                 280                 285             


Ser Ile Asp Ala Pro Ser Gln Pro Ser Asp Gln Glu Val Ala Gln Thr 
    290                 295                 300                 


Ser Val Asn Ser Ile Glu Ile Lys Ala Pro Gln Ala Ala Thr Pro Gln 
305                 310                 315                 320 


Gln Pro Val His Thr Glu Thr Ile Val His Thr Pro Met Thr Ala Val 
                325                 330                 335     


Pro Gln Thr Lys His Glu Ala Thr Phe Glu Val Pro Gln Gly Arg Asn 
            340                 345                 350         


Thr Gln Thr Ser Ala Thr Ala Asn Lys Glu Gln Ala Thr Glu Thr Val 
        355                 360                 365             


Asp Ala Pro Lys Ala Asn Gly Gln Val Gln Ser Glu Gln Val Ala Thr 
    370                 375                 380                 


Pro Gly Val Gln Gln Thr His Ser Met Ala Gln Gln Ser Glu Thr Pro 
385                 390                 395                 400 


Gln Pro Val Ala Glu Glu Thr Glu Thr Ile Thr Glu Asn His Val Val 
                405                 410                 415     


Asp Ile Asp Glu Ser Thr Thr Phe Gln Lys Ser Gly Tyr Leu Tyr Gly 
            420                 425                 430         


Val Ser Glu Ser Asp Thr Ser Gly Tyr Thr Glu Arg Glu Lys Arg Ala 
        435                 440                 445             


Ile Arg Arg Asn His Val Arg Glu Ala Glu Ala Leu Val Asn Gln Tyr 
    450                 455                 460                 


Val Glu Thr His Arg Tyr Gln Asp Arg Met Ala Ala Gln Gln Lys Val 
465                 470                 475                 480 


Asn Thr Leu Ser Lys Ala His Gln Lys Arg Phe Asn Lys Met Ile Asn 
                485                 490                 495     


Lys Ala Tyr Asn Gly Gln 
            500         


<210>  9
<211>  978
<212>  DNA
<213>  artificial sequence

<220>
<223>  S. pseudintermedius Gamma Hemolysin synthetic gene optimized for 
       expression in E.coli

<400>  9
atgaagatta gcaaagttat taaggcggcg accgcgacca gcgtggcgct gatgctgttt       60

agcaacccgg tgtatgcggc gaaccagatt accccggtga gcgagaagaa agttgacgat      120

aagatcaccc tgtacaaaac caccgcgacc gcggacagcg ataagctgaa catcgaccaa      180

ctgctgacct tcgcgtttat taaggataaa agctacgaca aggataccct ggtgctgaaa      240

gcggcgggta acattaacag cggctataaa agcccgaacc cgaacgacta catctatagc      300

agcttctact ggggtgcgaa gtataacgtg agcattagcg cggaaagcaa aggcgcggtt      360

aacgtggttg actacgcgcc gaagaaccaa aacgaggaat tccaggtgca aaacaccctg      420

ggttatagct ttggtggtga catcagcatt agcaagggtc tgagcggtgg cctgaacggc      480

agcgagagct ttagcgaaac catcaactac aaacaggaga gctatcgtac caccatcgac      540

aagcacaccg ataacaaaac cattggttgg ggcgttgaag cgcacaagat catgaacgcg      600

ggttggggtc cgtatggtcg tgacagcttc cacgatctgt atggcaacga gctgtttctg      660

ggtggccgtc agagcaaact gaacgcgggt caaaacttcc tgccgaccag ccagatgccg      720

ctgctggcgc gtggcaactt caacccggaa tttctgagcg tgctgagcca caagccgaac      780

ggtgcgaaaa ccagcaagat taaagttacc taccaacgtg agatggatga atacaccaac      840

tattggaacg gttttcactg gatgggcacc aactacaaaa accagaacaa cgcgaccttc      900

accagctttt atgagatcga ctgggaccaa cataccgtga aactgattaa gacccatagc      960

gatgagaaaa acccgagc                                                    978


<210>  10
<211>  326
<212>  PRT
<213>  artificial sequence

<220>
<223>  S. pseudintermedius attenuated Gamma Hemolysin protein

<400>  10

Met Lys Ile Ser Lys Val Ile Lys Ala Ala Thr Ala Thr Ser Val Ala 
1               5                   10                  15      


Leu Met Leu Phe Ser Asn Pro Val Tyr Ala Ala Asn Gln Ile Thr Pro 
            20                  25                  30          


Val Ser Glu Lys Lys Val Asp Asp Lys Ile Thr Leu Tyr Lys Thr Thr 
        35                  40                  45              


Ala Thr Ala Asp Ser Asp Lys Leu Asn Ile Asp Gln Leu Leu Thr Phe 
    50                  55                  60                  


Ala Phe Ile Lys Asp Lys Ser Tyr Asp Lys Asp Thr Leu Val Leu Lys 
65                  70                  75                  80  


Ala Ala Gly Asn Ile Asn Ser Gly Tyr Lys Ser Pro Asn Pro Asn Asp 
                85                  90                  95      


Tyr Ile Tyr Ser Ser Phe Tyr Trp Gly Ala Lys Tyr Asn Val Ser Ile 
            100                 105                 110         


Ser Ala Glu Ser Lys Gly Ala Val Asn Val Val Asp Tyr Ala Pro Lys 
        115                 120                 125             


Asn Gln Asn Glu Glu Phe Gln Val Gln Asn Thr Leu Gly Tyr Ser Phe 
    130                 135                 140                 


Gly Gly Asp Ile Ser Ile Ser Lys Gly Leu Ser Gly Gly Leu Asn Gly 
145                 150                 155                 160 


Ser Glu Ser Phe Ser Glu Thr Ile Asn Tyr Lys Gln Glu Ser Tyr Arg 
                165                 170                 175     


Thr Thr Ile Asp Lys His Thr Asp Asn Lys Thr Ile Gly Trp Gly Val 
            180                 185                 190         


Glu Ala His Lys Ile Met Asn Ala Gly Trp Gly Pro Tyr Gly Arg Asp 
        195                 200                 205             


Ser Phe His Asp Leu Tyr Gly Asn Glu Leu Phe Leu Gly Gly Arg Gln 
    210                 215                 220                 


Ser Lys Leu Asn Ala Gly Gln Asn Phe Leu Pro Thr Ser Gln Met Pro 
225                 230                 235                 240 


Leu Leu Ala Arg Gly Asn Phe Asn Pro Glu Phe Leu Ser Val Leu Ser 
                245                 250                 255     


His Lys Pro Asn Gly Ala Lys Thr Ser Lys Ile Lys Val Thr Tyr Gln 
            260                 265                 270         


Arg Glu Met Asp Glu Tyr Thr Asn Tyr Trp Asn Gly Phe His Trp Met 
        275                 280                 285             


Gly Thr Asn Tyr Lys Asn Gln Asn Asn Ala Thr Phe Thr Ser Phe Tyr 
    290                 295                 300                 


Glu Ile Asp Trp Asp Gln His Thr Val Lys Leu Ile Lys Thr His Ser 
305                 310                 315                 320 


Asp Glu Lys Asn Pro Ser 
                325     


<210>  11
<211>  717
<212>  DNA
<213>  artificial sequence

<220>
<223>  S. pseudintermedius Exotoxin 15 synthetic gene optimized for 
       expression in E.coli

<400>  11
ggatccaatg aaacagagca ccattctgaa agttagcctg gcagttggta ttctgaccac       60

cggtgtgggt attcatagcc aggcagcagc atttgcaagc gaagcacatg cacagaatgt      120

tcatagcgaa gcccagcagc tgaaagatta ttatagcaaa acctacttcg aatataacga      180

cgtgaccggt tatgtggaag ataacaataa actgagcgtt gtgacaccgc aagaaaccct      240

ggttagcatt agcctgctgg gtaaagatgc aagcacctat accgataaag aaaaagcata      300

tgatggtctg gccctgtttg ttgttccgga aggcaccgat cgtagcgcag aaaccaaaag      360

cattggtggt attaccaaac cgaatcagcg tagctattac gactatgtga aaaagccgaa      420

catcatcatc aacaaaaaat acggtgaact gaccagcagc atgagcacca atgatttttc      480

cattaacaaa gaagaagtga gcctgaaaga gctggatttt aaactgcgtc agaaactgat      540

taacgagtat ggcctgtatc agaatggcag cagtaatggt aaaatcgtga ttaaaatcgg      600

cgacaacgac aaagatatta tgccgctgga actgaacaaa aaactgcaag aacatgcaat      660

gagcgatacc gttgatgtga acaaaattca gcagattacc atcgacctgg cggccgc         717


<210>  12
<211>  236
<212>  PRT
<213>  artificial sequence

<220>
<223>  S. pseudintermedius attenuated Exotoxin 15 Protein

<400>  12

Met Lys Gln Ser Thr Ile Leu Lys Val Ser Leu Ala Val Gly Ile Leu 
1               5                   10                  15      


Thr Thr Gly Val Gly Ile His Ser Gln Ala Ala Ala Phe Ala Ser Glu 
            20                  25                  30          


Ala His Ala Gln Asn Val His Ser Glu Ala Gln Gln Leu Lys Asp Tyr 
        35                  40                  45              


Tyr Ser Lys Thr Tyr Phe Glu Tyr Asn Asp Val Thr Gly Tyr Val Glu 
    50                  55                  60                  


Asp Asn Asn Lys Leu Ser Val Val Thr Pro Gln Glu Thr Leu Val Ser 
65                  70                  75                  80  


Ile Ser Leu Leu Gly Lys Asp Ala Ser Thr Tyr Thr Asp Lys Glu Lys 
                85                  90                  95      


Ala Tyr Asp Gly Leu Ala Leu Phe Val Val Pro Glu Gly Thr Asp Arg 
            100                 105                 110         


Ser Ala Glu Thr Lys Ser Ile Gly Gly Ile Thr Lys Pro Asn Gln Arg 
        115                 120                 125             


Ser Tyr Tyr Asp Tyr Val Lys Lys Pro Asn Ile Ile Ile Asn Lys Lys 
    130                 135                 140                 


Tyr Gly Glu Leu Thr Ser Ser Met Ser Thr Asn Asp Phe Ser Ile Asn 
145                 150                 155                 160 


Lys Glu Glu Val Ser Leu Lys Glu Leu Asp Phe Lys Leu Arg Gln Lys 
                165                 170                 175     


Leu Ile Asn Glu Tyr Gly Leu Tyr Gln Asn Gly Ser Ser Asn Gly Lys 
            180                 185                 190         


Ile Val Ile Lys Ile Gly Asp Asn Asp Lys Asp Ile Met Pro Leu Glu 
        195                 200                 205             


Leu Asn Lys Lys Leu Gln Glu His Ala Met Ser Asp Thr Val Asp Val 
    210                 215                 220                 


Asn Lys Ile Gln Gln Ile Thr Ile Asp Leu Ala Ala 
225                 230                 235     


<210>  13
<211>  2023
<212>  DNA
<213>  artificial sequence

<220>
<223>  S. pseudintermedius Chimeric gene optimized for expression in 
       E.coli

<400>  13
cactataggg cgaattggcg gaaggccgtc aaggccgcat gcatgaggat ccaccgcagc       60

acagattacc gcaggtattg cactgcatca gagcaatctg aataccgcag ttccgcagac      120

caaacatgaa gcaacctttg aagtgccgca gggtcgtaat acccagacca gcgcaaccgc      180

aaataaagaa caggcaaccg aaaccgttga tgcaccgaaa gcaaatggtc aggttcagag      240

cgaacaggtt gcaacaccgg gtgttcagca gacccatagc atggcacagc agagcgaaac      300

accgcagccg gttgcagaag aaaccgaaac aattaccggt agcatggtga aaaacaaact      360

gctggcagca accctgagca ttagcctggt tctgccgctg attaccccgt atagcgaaga      420

agcaaaagca gcaaatacca ttgaagaaat tggtgaaggt gcccagatta tcaaacgtac      480

cgaagatgtt agcagccgta aatggggtgt ttttcagaac atccagttcg attttgtgaa      540

agacccgaaa tataacaaag acgccctgat catcaaaatg cagggtttta tcaaaagccg      600

taccagcttt accgatgtga aaggtaaagg ttatgaaagc accaaacgta tgctgtggcc      660

gtttcagtat aacattgcac tgaaaaccaa tgatccgaat gtgagcctga tcaattatct      720

gcctgcaaac aaaattgaga gcatcgatgt tagtcagacc ctgggttata atgttggtgg      780

taattttcag agcgcaccgc tgttaggtgg taaaggtgca tttaactaca gcaagaaaat      840

cagctacacc cagaaaaact atattagcga agttgcccag cagaacagca aaaacattcg      900

ttgggaagtt aaagccaaca gctttaatac cgaaaatggc caggttagcg catatgatcg      960

tcacctgttt gttcgtagcc cgattggtcc gaatgcacgt gatttttttg ttccgaatga     1020

tgaactgcct ccgctgattc agagcggttt taatccggca tttattgcaa ccgttagcca     1080

cgaaaaagat aaaggtgata ccagcgaatt tgaaattgcc tatggtcgca atctggatat     1140

tacctatgca accttttttc cgcgtaccgg tatttttgca gaacgtcgtc ataatgcact     1200

gatgaatcgt aatctggtga ccaaatatga ggtgaactgg aaaacccatg agatcaaagt     1260

gaaaggccat aacgcagcaa aaaaagcaca gccggaagca accagcattg atcgtgcaag     1320

ccatgaaacc gcagcaacca cacataccat tctgcatacc aatgcaattg ccggtcgtat     1380

ggttgaagaa aaagatcgtg ttctgggtta tgatgcaatg accgcaggca atgcagaatt     1440

tgcctttggt tatgatcagc tgaaaaaact ggaaggcatg ctgaattttc cgattgtgag     1500

cagcaatgtg tacaaacacg aagatccgag ctttgccgaa gaaggtgata ataacgccga     1560

agccaaaaaa aacgccttta gtgaagttgt gaaactgccg aatctgagcg aagaacagcg     1620

taatggtttt attcagagcc tgaaagcagc accgagcacc agccaggatg tgctgaatga     1680

ggcaaaaaaa ctgaatgata gccaagaagg tagccagcct gcaccggatt atagtgatga     1740

aaaaaagaac gcgttttatg aaatccagtc attaaaagcc gcaccgtcag ttagcgcaaa     1800

tattctggtt gaagcgaaaa acatgaacgt taatcagacc ccgacacaac cggcaccgag     1860

ttttgatgaa gcaaaaaaga atgccttcta cgagattgtg aatcaggcca aacctgatta     1920

taacgaggca aagaaaaatg cattctatga aatactgcat ctgccgaatg cggccgctca     1980

tgcctgggcc tcatgggcct tccgctcact gcccgctttc cag                       2023


<210>  14
<211>  639
<212>  PRT
<213>  artificial sequence

<220>
<223>  S. pseudintermedius attenuated chimeric protein

<400>  14

Thr Ala Ala Gln Ile Thr Ala Gly Ile Ala Leu His Gln Ser Asn Leu 
1               5                   10                  15      


Asn Thr Ala Val Pro Gln Thr Lys His Glu Ala Thr Phe Glu Val Pro 
            20                  25                  30          


Gln Gly Arg Asn Thr Gln Thr Ser Ala Thr Ala Asn Lys Glu Gln Ala 
        35                  40                  45              


Thr Glu Thr Val Asp Ala Pro Lys Ala Asn Gly Gln Val Gln Ser Glu 
    50                  55                  60                  


Gln Val Ala Thr Pro Gly Val Gln Gln Thr His Ser Met Ala Gln Gln 
65                  70                  75                  80  


Ser Glu Thr Pro Gln Pro Val Ala Glu Glu Thr Glu Thr Ile Thr Gly 
                85                  90                  95      


Ser Met Val Lys Asn Lys Leu Leu Ala Ala Thr Leu Ser Ile Ser Leu 
            100                 105                 110         


Val Leu Pro Leu Ile Thr Pro Tyr Ser Glu Glu Ala Lys Ala Ala Asn 
        115                 120                 125             


Thr Ile Glu Glu Ile Gly Glu Gly Ala Gln Ile Ile Lys Arg Thr Glu 
    130                 135                 140                 


Asp Val Ser Ser Arg Lys Trp Gly Val Phe Gln Asn Ile Gln Phe Asp 
145                 150                 155                 160 


Phe Val Lys Asp Pro Lys Tyr Asn Lys Asp Ala Leu Ile Ile Lys Met 
                165                 170                 175     


Gln Gly Phe Ile Lys Ser Arg Thr Ser Phe Thr Asp Val Lys Gly Lys 
            180                 185                 190         


Gly Tyr Glu Ser Thr Lys Arg Met Leu Trp Pro Phe Gln Tyr Asn Ile 
        195                 200                 205             


Ala Leu Lys Thr Asn Asp Pro Asn Val Ser Leu Ile Asn Tyr Leu Pro 
    210                 215                 220                 


Ala Asn Lys Ile Glu Ser Ile Asp Val Ser Gln Thr Leu Gly Tyr Asn 
225                 230                 235                 240 


Val Gly Gly Asn Phe Gln Ser Ala Pro Leu Leu Gly Gly Lys Gly Ala 
                245                 250                 255     


Phe Asn Tyr Ser Lys Lys Ile Ser Tyr Thr Gln Lys Asn Tyr Ile Ser 
            260                 265                 270         


Glu Val Ala Gln Gln Asn Ser Lys Asn Ile Arg Trp Glu Val Lys Ala 
        275                 280                 285             


Asn Ser Phe Asn Thr Glu Asn Gly Gln Val Ser Ala Tyr Asp Arg His 
    290                 295                 300                 


Leu Phe Val Arg Ser Pro Ile Gly Pro Asn Ala Arg Asp Phe Phe Val 
305                 310                 315                 320 


Pro Asn Asp Glu Leu Pro Pro Leu Ile Gln Ser Gly Phe Asn Pro Ala 
                325                 330                 335     


Phe Ile Ala Thr Val Ser His Glu Lys Asp Lys Gly Asp Thr Ser Glu 
            340                 345                 350         


Phe Glu Ile Ala Tyr Gly Arg Asn Leu Asp Ile Thr Tyr Ala Thr Phe 
        355                 360                 365             


Phe Pro Arg Thr Gly Ile Phe Ala Glu Arg Arg His Asn Ala Leu Met 
    370                 375                 380                 


Asn Arg Asn Leu Val Thr Lys Tyr Glu Val Asn Trp Lys Thr His Glu 
385                 390                 395                 400 


Ile Lys Val Lys Gly His Asn Ala Ala Lys Lys Ala Gln Pro Glu Ala 
                405                 410                 415     


Thr Ser Ile Asp Arg Ala Ser His Glu Thr Ala Ala Thr Thr His Thr 
            420                 425                 430         


Ile Leu His Thr Asn Ala Ile Ala Gly Arg Met Val Glu Glu Lys Asp 
        435                 440                 445             


Arg Val Leu Gly Tyr Asp Ala Met Thr Ala Gly Asn Ala Glu Phe Ala 
    450                 455                 460                 


Phe Gly Tyr Asp Gln Leu Lys Lys Leu Glu Gly Met Leu Asn Phe Pro 
465                 470                 475                 480 


Ile Val Ser Ser Asn Val Tyr Lys His Glu Asp Pro Ser Phe Ala Glu 
                485                 490                 495     


Glu Gly Asp Asn Asn Ala Glu Ala Lys Lys Asn Ala Phe Ser Glu Val 
            500                 505                 510         


Val Lys Leu Pro Asn Leu Ser Glu Glu Gln Arg Asn Gly Phe Ile Gln 
        515                 520                 525             


Ser Leu Lys Ala Ala Pro Ser Thr Ser Gln Asp Val Leu Asn Glu Ala 
    530                 535                 540                 


Lys Lys Leu Asn Asp Ser Gln Glu Gly Ser Gln Pro Ala Pro Asp Tyr 
545                 550                 555                 560 


Ser Asp Glu Lys Lys Asn Ala Phe Tyr Glu Ile Gln Ser Leu Lys Ala 
                565                 570                 575     


Ala Pro Ser Val Ser Ala Asn Ile Leu Val Glu Ala Lys Asn Met Asn 
            580                 585                 590         


Val Asn Gln Thr Pro Thr Gln Pro Ala Pro Ser Phe Asp Glu Ala Lys 
        595                 600                 605             


Lys Asn Ala Phe Tyr Glu Ile Val Asn Gln Ala Lys Pro Asp Tyr Asn 
    610                 615                 620                 


Glu Ala Lys Lys Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn 
625                 630                 635                 


