                         SEQUENCE LISTING

<110>  OncoMed Pharmaceuticals, Inc.
       Gurney, Austin L
       Srivastava, Minu K
 
<120>  CD40-BINDING AGENTS AND USES THEREOF

<130>  2293.170PC01/PAC/LMB

<140>  62/543,734
<141>  2017-08-10

<160>  82    

<170>  PatentIn version 3.5

<210>  1
<211>  260
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CD40L amino acid sequence

<400>  1

Met Ile Glu Thr Tyr Ser Gln Pro Ser Pro Arg Ser Val Ala Thr Gly 
1               5                   10                  15      


Leu Pro Ala Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Val Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Val Glu Glu Glu Val Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Ile Lys Lys Leu Lys Arg Cys Asn Lys Gly Glu Gly Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Met Arg Arg Gln Phe Glu Asp Leu Val Lys 
                85                  90                  95      


Asp Ile Thr Leu Asn Lys Glu Glu Lys Lys Glu Asn Ser Phe Glu Met 
            100                 105                 110         


Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser Glu 
        115                 120                 125             


Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr 
    130                 135                 140                 


Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu 
145                 150                 155                 160 


Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe 
                165                 170                 175     


Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu 
            180                 185                 190         


Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala 
        195                 200                 205             


Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu 
    210                 215                 220                 


Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val 
225                 230                 235                 240 


Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe Gly 
                245                 250                 255     


Leu Leu Lys Leu 
            260 


<210>  2
<211>  214
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CD40L extracellular domain amino acid sequence (aa 47-260)

<400>  2

His Arg Arg Leu Asp Lys Val Glu Glu Glu Val Asn Leu His Glu Asp 
1               5                   10                  15      


Phe Val Phe Ile Lys Lys Leu Lys Arg Cys Asn Lys Gly Glu Gly Ser 
            20                  25                  30          


Leu Ser Leu Leu Asn Cys Glu Glu Met Arg Arg Gln Phe Glu Asp Leu 
        35                  40                  45              


Val Lys Asp Ile Thr Leu Asn Lys Glu Glu Lys Lys Glu Asn Ser Phe 
    50                  55                  60                  


Glu Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val 
65                  70                  75                  80  


Ser Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys 
                85                  90                  95      


Gly Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys 
            100                 105                 110         


Gln Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val 
        115                 120                 125             


Thr Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val 
    130                 135                 140                 


Gly Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys 
145                 150                 155                 160 


Ala Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val 
                165                 170                 175     


His Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val 
            180                 185                 190         


Asn Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser 
        195                 200                 205             


Phe Gly Leu Leu Lys Leu 
    210                 


<210>  3
<211>  71
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CD40L Stalk Region

<400>  3

His Arg Arg Leu Asp Lys Val Glu Glu Glu Val Asn Leu His Glu Asp 
1               5                   10                  15      


Phe Val Phe Ile Lys Lys Leu Lys Arg Cys Asn Lys Gly Glu Gly Ser 
            20                  25                  30          


Leu Ser Leu Leu Asn Cys Glu Glu Met Arg Arg Gln Phe Glu Asp Leu 
        35                  40                  45              


Val Lys Asp Ile Thr Leu Asn Lys Glu Glu Lys Lys Glu Asn Ser Phe 
    50                  55                  60                  


Glu Met Gln Arg Gly Asp Glu 
65                  70      


<210>  4
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CD40L extracellular domain without stalk region amino acid 
       sequence

<400>  4

Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn Ala 
1               5                   10                  15      


Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser 
            20                  25                  30          


Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu 
        35                  40                  45              


Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu 
    50                  55                  60                  


Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser 
65                  70                  75                  80  


Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser 
                85                  90                  95      


Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe Glu 
            100                 105                 110         


Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln 
        115                 120                 125             


Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu 
    130                 135                 140             


<210>  5
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CD40L Stalk - Fragment 1

<400>  5

Met Gln Arg Gly Asp Glu 
1               5       


<210>  6
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse CD40L extracellular domain with stalk fragment 1 (aa 
       112-260)

<400>  6

Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser 
1               5                   10                  15      


Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly 
65                  70                  75                  80  


Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu 
145                 


<210>  7
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse single chain CD40L trimer amino acid sequence without 
       signal sequence

<400>  7

Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser 
1               5                   10                  15      


Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly 
65                  70                  75                  80  


Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val 
        195                 200                 205             


Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg 
    210                 215                 220                 


Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu 
                245                 250                 255     


Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile His Arg Val 
        275                 280                 285             


Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu Met Gln Arg Gly Asp Glu 
    290                 295                 300                 


Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn Ala 
305                 310                 315                 320 


Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser 
                325                 330                 335     


Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu 
            340                 345                 350         


Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser 
    370                 375                 380                 


Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln 
            420                 425                 430         


Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu 
        435                 440                 445         


<210>  8
<211>  701
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  340F1 Mouse single chain CD40L trimer-Fc (mIgG2a) with predicted 
       signal sequence (aa 1-27)

<400>  8

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Ser Pro Pro Cys Lys 
            20                  25                  30          


Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro 
        35                  40                  45              


Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser Pro Ile Val Thr 
    50                  55                  60                  


Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln Ile Ser 
65                  70                  75                  80  


Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln Thr Gln Thr His 
                85                  90                  95      


Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val Ser Ala Leu Pro Ile 
            100                 105                 110         


Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn 
        115                 120                 125             


Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile Ser Lys Pro Lys 
    130                 135                 140                 


Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro Pro Pro Glu Glu 
145                 150                 155                 160 


Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met Val Thr Asp Phe 
                165                 170                 175     


Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn Gly Lys Thr Glu 
            180                 185                 190         


Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser Asp Gly Ser Tyr 
        195                 200                 205             


Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn Trp Val Glu Arg 
    210                 215                 220                 


Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu His Asn His His 
225                 230                 235                 240 


Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys Gly Arg Ala Met Gln 
                245                 250                 255     


Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala 
            260                 265                 270         


Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr 
        275                 280                 285             


Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr 
    290                 295                 300                 


Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys 
305                 310                 315                 320 


Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp 
                325                 330                 335     


Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn 
            340                 345                 350         


Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly 
        355                 360                 365             


Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr 
    370                 375                 380                 


Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu 
385                 390                 395                 400 


Leu Lys Leu Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His 
                405                 410                 415     


Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala 
            420                 425                 430         


Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn 
        435                 440                 445             


Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr 
    450                 455                 460                 


Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe 
465                 470                 475                 480 


Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu 
                485                 490                 495     


Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln 
            500                 505                 510         


Ser Val His Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val 
        515                 520                 525             


Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe 
    530                 535                 540                 


Ser Ser Phe Gly Leu Leu Lys Leu Met Gln Arg Gly Asp Glu Asp Pro 
545                 550                 555                 560 


Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser 
                565                 570                 575     


Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu 
            580                 585                 590         


Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu 
        595                 600                 605             


Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser 
    610                 615                 620                 


Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly 
625                 630                 635                 640 


Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln 
                645                 650                 655     


Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln 
            660                 665                 670         


Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile 
        675                 680                 685             


His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu 
    690                 695                 700     


<210>  9
<211>  674
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  340F1 Mouse single chain CD40L trimer-Fc (mIgG2a) without signal 
       sequence

<400>  9

Ser Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser 
1               5                   10                  15      


Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu 
            20                  25                  30          


Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro 
        35                  40                  45              


Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala 
    50                  55                  60                  


Gln Thr Gln Thr His Arg Glu Asp Tyr Asn Ser Thr Leu Arg Val Val 
65                  70                  75                  80  


Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe 
                85                  90                  95      


Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr 
            100                 105                 110         


Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu 
        115                 120                 125             


Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys 
    130                 135                 140                 


Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn 
145                 150                 155                 160 


Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp 
                165                 170                 175     


Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys 
            180                 185                 190         


Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly 
        195                 200                 205             


Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys 
    210                 215                 220                 


Gly Arg Ala Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His 
225                 230                 235                 240 


Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala 
                245                 250                 255     


Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn 
            260                 265                 270         


Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr 
        275                 280                 285             


Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe 
    290                 295                 300                 


Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu 
305                 310                 315                 320 


Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln 
                325                 330                 335     


Ser Val His Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val 
            340                 345                 350         


Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe 
        355                 360                 365             


Ser Ser Phe Gly Leu Leu Lys Leu Met Gln Arg Gly Asp Glu Asp Pro 
    370                 375                 380                 


Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser 
385                 390                 395                 400 


Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu 
                405                 410                 415     


Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu 
            420                 425                 430         


Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser 
        435                 440                 445             


Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly 
    450                 455                 460                 


Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln 
465                 470                 475                 480 


Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln 
                485                 490                 495     


Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile 
            500                 505                 510         


His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu Met Gln Arg 
        515                 520                 525             


Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala Asn 
    530                 535                 540                 


Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr 
545                 550                 555                 560 


Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val 
                565                 570                 575     


Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser 
            580                 585                 590         


Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu 
        595                 600                 605             


Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr 
    610                 615                 620                 


His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly 
625                 630                 635                 640 


Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu 
                645                 650                 655     


Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu 
            660                 665                 670         


Lys Leu 
        


<210>  10
<211>  701
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  340F2 Mouse single chain CD40L trimer (C term)-Fc (N term) 
       (deglycosylated mIgG2a) with predicted signal sequence (aa 1-27)

<400>  10

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Ser Pro Pro Cys Lys 
            20                  25                  30          


Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser Val Phe Ile Phe Pro 
        35                  40                  45              


Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser Pro Ile Val Thr 
    50                  55                  60                  


Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln Ile Ser 
65                  70                  75                  80  


Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln Thr Gln Thr His 
                85                  90                  95      


Arg Glu Asp Tyr Ala Ser Thr Leu Arg Val Val Ser Ala Leu Pro Ile 
            100                 105                 110         


Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys Val Asn 
        115                 120                 125             


Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile Ser Lys Pro Lys 
    130                 135                 140                 


Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro Pro Pro Glu Glu 
145                 150                 155                 160 


Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met Val Thr Asp Phe 
                165                 170                 175     


Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn Gly Lys Thr Glu 
            180                 185                 190         


Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser Asp Gly Ser Tyr 
        195                 200                 205             


Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn Trp Val Glu Arg 
    210                 215                 220                 


Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu His Asn His His 
225                 230                 235                 240 


Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys Gly Arg Ala Met Gln 
                245                 250                 255     


Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala 
            260                 265                 270         


Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr 
        275                 280                 285             


Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr 
    290                 295                 300                 


Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys 
305                 310                 315                 320 


Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp 
                325                 330                 335     


Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn 
            340                 345                 350         


Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly 
        355                 360                 365             


Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr 
    370                 375                 380                 


Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu 
385                 390                 395                 400 


Leu Lys Leu Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His 
                405                 410                 415     


Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala 
            420                 425                 430         


Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn 
        435                 440                 445             


Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr 
    450                 455                 460                 


Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe 
465                 470                 475                 480 


Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu 
                485                 490                 495     


Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln 
            500                 505                 510         


Ser Val His Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val 
        515                 520                 525             


Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe 
    530                 535                 540                 


Ser Ser Phe Gly Leu Leu Lys Leu Met Gln Arg Gly Asp Glu Asp Pro 
545                 550                 555                 560 


Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser 
                565                 570                 575     


Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu 
            580                 585                 590         


Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu 
        595                 600                 605             


Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser 
    610                 615                 620                 


Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly 
625                 630                 635                 640 


Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln 
                645                 650                 655     


Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln 
            660                 665                 670         


Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile 
        675                 680                 685             


His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu 
    690                 695                 700     


<210>  11
<211>  674
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  340F2 Mouse single chain CD40L trimer (C term)-Fc (N term) 
       (deglycosylated mIgG2a) without signal sequence

<400>  11

Ser Pro Pro Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser 
1               5                   10                  15      


Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu 
            20                  25                  30          


Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro 
        35                  40                  45              


Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala 
    50                  55                  60                  


Gln Thr Gln Thr His Arg Glu Asp Tyr Ala Ser Thr Leu Arg Val Val 
65                  70                  75                  80  


Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe 
                85                  90                  95      


Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr 
            100                 105                 110         


Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu 
        115                 120                 125             


Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys 
    130                 135                 140                 


Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn 
145                 150                 155                 160 


Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp 
                165                 170                 175     


Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys 
            180                 185                 190         


Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly 
        195                 200                 205             


Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys 
    210                 215                 220                 


Gly Arg Ala Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His 
225                 230                 235                 240 


Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala 
                245                 250                 255     


Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn 
            260                 265                 270         


Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr 
        275                 280                 285             


Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe 
    290                 295                 300                 


Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu 
305                 310                 315                 320 


Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln 
                325                 330                 335     


Ser Val His Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val 
            340                 345                 350         


Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe 
        355                 360                 365             


Ser Ser Phe Gly Leu Leu Lys Leu Met Gln Arg Gly Asp Glu Asp Pro 
    370                 375                 380                 


Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser 
385                 390                 395                 400 


Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu 
                405                 410                 415     


Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu 
            420                 425                 430         


Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser 
        435                 440                 445             


Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly 
    450                 455                 460                 


Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln 
465                 470                 475                 480 


Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln 
                485                 490                 495     


Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile 
            500                 505                 510         


His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu Met Gln Arg 
        515                 520                 525             


Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala Asn 
    530                 535                 540                 


Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr 
545                 550                 555                 560 


Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val 
                565                 570                 575     


Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser 
            580                 585                 590         


Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu 
        595                 600                 605             


Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr 
    610                 615                 620                 


His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly 
625                 630                 635                 640 


Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu 
                645                 650                 655     


Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu 
            660                 665                 670         


Lys Leu 
        


<210>  12
<211>  261
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L (TNFSF5) amino acid sequence

<400>  12

Met Ile Glu Thr Tyr Asn Gln Thr Ser Pro Arg Ser Ala Ala Thr Gly 
1               5                   10                  15      


Leu Pro Ile Ser Met Lys Ile Phe Met Tyr Leu Leu Thr Val Phe Leu 
            20                  25                  30          


Ile Thr Gln Met Ile Gly Ser Ala Leu Phe Ala Val Tyr Leu His Arg 
        35                  40                  45              


Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp Phe Val 
    50                  55                  60                  


Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser Leu Ser 
65                  70                  75                  80  


Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe Val Lys 
                85                  90                  95      


Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Ser Phe Glu 
            100                 105                 110         


Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
        115                 120                 125             


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
    130                 135                 140                 


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
145                 150                 155                 160 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
                165                 170                 175     


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
            180                 185                 190         


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
        195                 200                 205             


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
    210                 215                 220                 


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
225                 230                 235                 240 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
                245                 250                 255     


Gly Leu Leu Lys Leu 
            260     


<210>  13
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L extracellular domain amino acid sequence (aa 47-261)

<400>  13

His Arg Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp 
1               5                   10                  15      


Phe Val Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser 
            20                  25                  30          


Leu Ser Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe 
        35                  40                  45              


Val Lys Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Ser 
    50                  55                  60                  


Phe Glu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val 
65                  70                  75                  80  


Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu 
                85                  90                  95      


Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly 
            100                 105                 110         


Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln 
        115                 120                 125             


Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile 
    130                 135                 140                 


Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu 
145                 150                 155                 160 


Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser 
                165                 170                 175     


Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe 
            180                 185                 190         


Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr 
        195                 200                 205             


Ser Phe Gly Leu Leu Lys Leu 
    210                 215 


<210>  14
<211>  72
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L Stalk Region

<400>  14

His Arg Arg Leu Asp Lys Ile Glu Asp Glu Arg Asn Leu His Glu Asp 
1               5                   10                  15      


Phe Val Phe Met Lys Thr Ile Gln Arg Cys Asn Thr Gly Glu Arg Ser 
            20                  25                  30          


Leu Ser Leu Leu Asn Cys Glu Glu Ile Lys Ser Gln Phe Glu Gly Phe 
        35                  40                  45              


Val Lys Asp Ile Met Leu Asn Lys Glu Glu Thr Lys Lys Glu Asn Ser 
    50                  55                  60                  


Phe Glu Met Gln Lys Gly Asp Gln 
65                  70          


<210>  15
<211>  143
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L extracellular domain without stalk region amino acid 
       sequence

<400>  15

Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
1               5                   10                  15      


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
            20                  25                  30          


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
        35                  40                  45              


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
    50                  55                  60                  


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
65                  70                  75                  80  


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
                85                  90                  95      


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
            100                 105                 110         


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
        115                 120                 125             


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
    130                 135                 140             


<210>  16
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L Stalk Region - Fragment 1

<400>  16

Met Gln Lys Gly Asp Gln 
1               5       


<210>  17
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L Stalk Region - Fragment 2

<400>  17

Phe Glu Met Gln Lys Gly Asp Gln 
1               5               


<210>  18
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L Stalk Region - Fragment 3

<400>  18

Glu Met Gln Lys Gly Asp Gln 
1               5           


<210>  19
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L Stalk Region - Fragment 4

<400>  19

Gln Lys Gly Asp Gln 
1               5   


<210>  20
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L Stalk Region - Fragment 5

<400>  20

Lys Gly Asp Gln 
1               


<210>  21
<211>  140
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L TNF homology domain

<400>  21

Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val 
1               5                   10                  15      


Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val 
            20                  25                  30          


Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr 
        35                  40                  45              


Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser 
    50                  55                  60                  


Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe 
65                  70                  75                  80  


Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro 
                85                  90                  95      


Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro 
            100                 105                 110         


Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His 
        115                 120                 125             


Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
    130                 135                 140 


<210>  22
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L extracellular with stalk fragment 1 (aa 113-261)

<400>  22

Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu 
145                 


<210>  23
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L extracellular domain with stalk fragment 2 (aa 
       111-261)

<400>  23

Phe Glu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val 
1               5                   10                  15      


Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu 
            20                  25                  30          


Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly 
        35                  40                  45              


Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln 
    50                  55                  60                  


Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile 
65                  70                  75                  80  


Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu 
                85                  90                  95      


Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser 
            100                 105                 110         


Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe 
        115                 120                 125             


Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr 
    130                 135                 140                 


Ser Phe Gly Leu Leu Lys Leu 
145                 150     


<210>  24
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L extracellular domain with stalk fragment 3 (aa 
       112-261)

<400>  24

Glu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile 
1               5                   10                  15      


Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys 
            20                  25                  30          


Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys 
        35                  40                  45              


Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val 
    50                  55                  60                  


Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala 
65                  70                  75                  80  


Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg 
                85                  90                  95      


Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile 
            100                 105                 110         


His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val 
        115                 120                 125             


Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser 
    130                 135                 140                 


Phe Gly Leu Leu Lys Leu 
145                 150 


<210>  25
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L extracellular domain with stalk fragment 4 (aa 
       114-261)

<400>  25

Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu 
1               5                   10                  15      


Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr 
            20                  25                  30          


Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu 
        35                  40                  45              


Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe 
    50                  55                  60                  


Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu 
65                  70                  75                  80  


Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala 
                85                  90                  95      


Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu 
            100                 105                 110         


Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val 
        115                 120                 125             


Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly 
    130                 135                 140                 


Leu Leu Lys Leu 
145             


<210>  26
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L extracellular domain with stalk fragment 5 (aa 
       115-261)

<400>  26

Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala 
1               5                   10                  15      


Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr 
            20                  25                  30          


Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr 
        35                  40                  45              


Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys 
    50                  55                  60                  


Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys 
65                  70                  75                  80  


Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn 
                85                  90                  95      


Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly 
            100                 105                 110         


Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr 
        115                 120                 125             


Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu 
    130                 135                 140                 


Leu Lys Leu 
145         


<210>  27
<211>  474
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer amino acid sequence with signal 
       sequence (aa 1-27)

<400>  27

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Met Gln Lys Gly Asp 
            20                  25                  30          


Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys 
        35                  40                  45              


Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser 
    50                  55                  60                  


Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg 
65                  70                  75                  80  


Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg 
                85                  90                  95      


Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser 
            100                 105                 110         


Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser 
        115                 120                 125             


Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe 
    130                 135                 140                 


Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser 
145                 150                 155                 160 


Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
                165                 170                 175     


Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
            180                 185                 190         


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
        195                 200                 205             


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
    210                 215                 220                 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
225                 230                 235                 240 


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
                245                 250                 255     


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
            260                 265                 270         


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
        275                 280                 285             


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
    290                 295                 300                 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
305                 310                 315                 320 


Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala 
                325                 330                 335     


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
            340                 345                 350         


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
        355                 360                 365             


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
    370                 375                 380                 


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
385                 390                 395                 400 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
                405                 410                 415     


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
            420                 425                 430         


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
        435                 440                 445             


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
    450                 455                 460                 


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
465                 470                 


<210>  28
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer amino acid sequence without 
       signal sequence

<400>  28

Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
        195                 200                 205             


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
    210                 215                 220                 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
                245                 250                 255     


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
        275                 280                 285             


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln 
    290                 295                 300                 


Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
305                 310                 315                 320 


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
                325                 330                 335     


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
            340                 345                 350         


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
    370                 375                 380                 


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
            420                 425                 430         


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
        435                 440                 445         


<210>  29
<211>  701
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer-Fc (hIgG1) amino acid sequence 
       with signal sequence (aa 1-27)

<400>  29

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Met Gln Lys Gly Asp 
            20                  25                  30          


Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys 
        35                  40                  45              


Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser 
    50                  55                  60                  


Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg 
65                  70                  75                  80  


Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg 
                85                  90                  95      


Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser 
            100                 105                 110         


Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser 
        115                 120                 125             


Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe 
    130                 135                 140                 


Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser 
145                 150                 155                 160 


Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
                165                 170                 175     


Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
            180                 185                 190         


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
        195                 200                 205             


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
    210                 215                 220                 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
225                 230                 235                 240 


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
                245                 250                 255     


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
            260                 265                 270         


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
        275                 280                 285             


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
    290                 295                 300                 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
305                 310                 315                 320 


Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala 
                325                 330                 335     


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
            340                 345                 350         


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
        355                 360                 365             


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
    370                 375                 380                 


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
385                 390                 395                 400 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
                405                 410                 415     


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
            420                 425                 430         


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
        435                 440                 445             


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
    450                 455                 460                 


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Asp Lys Thr His Thr Cys 
465                 470                 475                 480 


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
                485                 490                 495     


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
            500                 505                 510         


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
        515                 520                 525             


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
    530                 535                 540                 


Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu 
545                 550                 555                 560 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
                565                 570                 575     


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
            580                 585                 590         


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
        595                 600                 605             


Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
    610                 615                 620                 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
625                 630                 635                 640 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
                645                 650                 655     


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
            660                 665                 670         


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
        675                 680                 685             


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    690                 695                 700     


<210>  30
<211>  674
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer-Fc (hIgG1) amino acid sequence 
       without signal sequence

<400>  30

Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
        195                 200                 205             


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
    210                 215                 220                 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
                245                 250                 255     


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
        275                 280                 285             


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln 
    290                 295                 300                 


Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
305                 310                 315                 320 


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
                325                 330                 335     


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
            340                 345                 350         


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
    370                 375                 380                 


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
            420                 425                 430         


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Asp 
        435                 440                 445             


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
    450                 455                 460                 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
465                 470                 475                 480 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
                485                 490                 495     


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
            500                 505                 510         


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
        515                 520                 525             


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
    530                 535                 540                 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
545                 550                 555                 560 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
                565                 570                 575     


Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
            580                 585                 590         


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
        595                 600                 605             


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
    610                 615                 620                 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
625                 630                 635                 640 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
                645                 650                 655     


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
            660                 665                 670         


Gly Lys 
        


<210>  31
<211>  703
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer-Fc (hIgG2) amino acid sequence 
       with signal sequence (aa 1-27)

<400>  31

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Met Gln Lys Gly Asp 
            20                  25                  30          


Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys 
        35                  40                  45              


Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser 
    50                  55                  60                  


Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg 
65                  70                  75                  80  


Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg 
                85                  90                  95      


Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser 
            100                 105                 110         


Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser 
        115                 120                 125             


Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe 
    130                 135                 140                 


Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser 
145                 150                 155                 160 


Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
                165                 170                 175     


Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
            180                 185                 190         


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
        195                 200                 205             


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
    210                 215                 220                 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
225                 230                 235                 240 


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
                245                 250                 255     


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
            260                 265                 270         


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
        275                 280                 285             


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
    290                 295                 300                 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
305                 310                 315                 320 


Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala 
                325                 330                 335     


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
            340                 345                 350         


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
        355                 360                 365             


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
    370                 375                 380                 


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
385                 390                 395                 400 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
                405                 410                 415     


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
            420                 425                 430         


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
        435                 440                 445             


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
    450                 455                 460                 


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Val Glu Arg Lys Ser Cys 
465                 470                 475                 480 


Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val 
                485                 490                 495     


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
            500                 505                 510         


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
        515                 520                 525             


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
    530                 535                 540                 


Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val Ser 
545                 550                 555                 560 


Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
                565                 570                 575     


Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr Ile 
            580                 585                 590         


Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
        595                 600                 605             


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
    610                 615                 620                 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
625                 630                 635                 640 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp Ser 
                645                 650                 655     


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
            660                 665                 670         


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
        675                 680                 685             


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    690                 695                 700             


<210>  32
<211>  676
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer-Fc (hIgG2) amino acid sequence 
       without signal sequence

<400>  32

Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
        195                 200                 205             


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
    210                 215                 220                 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
                245                 250                 255     


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
        275                 280                 285             


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln 
    290                 295                 300                 


Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
305                 310                 315                 320 


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
                325                 330                 335     


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
            340                 345                 350         


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
    370                 375                 380                 


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
            420                 425                 430         


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Val 
        435                 440                 445             


Glu Arg Lys Ser Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val 
    450                 455                 460                 


Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
465                 470                 475                 480 


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
                485                 490                 495     


His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu 
            500                 505                 510         


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr 
        515                 520                 525             


Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn 
    530                 535                 540                 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro 
545                 550                 555                 560 


Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln 
                565                 570                 575     


Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val 
            580                 585                 590         


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
        595                 600                 605             


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
    610                 615                 620                 


Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
625                 630                 635                 640 


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
                645                 650                 655     


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
            660                 665                 670         


Ser Pro Gly Lys 
        675     


<210>  33
<211>  703
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer-Fc (deglycosylated hIgG2) amino 
       acid sequence with signal sequence (aa 1-27)

<400>  33

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Met Gln Lys Gly Asp 
            20                  25                  30          


Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys 
        35                  40                  45              


Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser 
    50                  55                  60                  


Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg 
65                  70                  75                  80  


Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg 
                85                  90                  95      


Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser 
            100                 105                 110         


Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser 
        115                 120                 125             


Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe 
    130                 135                 140                 


Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser 
145                 150                 155                 160 


Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
                165                 170                 175     


Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
            180                 185                 190         


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
        195                 200                 205             


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
    210                 215                 220                 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
225                 230                 235                 240 


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
                245                 250                 255     


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
            260                 265                 270         


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
        275                 280                 285             


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
    290                 295                 300                 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
305                 310                 315                 320 


Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala 
                325                 330                 335     


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
            340                 345                 350         


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
        355                 360                 365             


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
    370                 375                 380                 


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
385                 390                 395                 400 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
                405                 410                 415     


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
            420                 425                 430         


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
        435                 440                 445             


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
    450                 455                 460                 


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Val Glu Arg Lys Ser Cys 
465                 470                 475                 480 


Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser Val 
                485                 490                 495     


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
            500                 505                 510         


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
        515                 520                 525             


Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
    530                 535                 540                 


Thr Lys Pro Arg Glu Glu Gln Phe Ala Ser Thr Phe Arg Val Val Ser 
545                 550                 555                 560 


Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
                565                 570                 575     


Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr Ile 
            580                 585                 590         


Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
        595                 600                 605             


Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
    610                 615                 620                 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
625                 630                 635                 640 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp Ser 
                645                 650                 655     


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
            660                 665                 670         


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
        675                 680                 685             


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    690                 695                 700             


<210>  34
<211>  676
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer-Fc (deglycosylated hIgG2) amino 
       acid sequence without signal sequence

<400>  34

Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
        195                 200                 205             


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
    210                 215                 220                 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
                245                 250                 255     


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
        275                 280                 285             


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln 
    290                 295                 300                 


Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
305                 310                 315                 320 


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
                325                 330                 335     


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
            340                 345                 350         


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
    370                 375                 380                 


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
            420                 425                 430         


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Val 
        435                 440                 445             


Glu Arg Lys Ser Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val 
    450                 455                 460                 


Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
465                 470                 475                 480 


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
                485                 490                 495     


His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu 
            500                 505                 510         


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Ala Ser Thr 
        515                 520                 525             


Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn 
    530                 535                 540                 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro 
545                 550                 555                 560 


Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln 
                565                 570                 575     


Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val 
            580                 585                 590         


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
        595                 600                 605             


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
    610                 615                 620                 


Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
625                 630                 635                 640 


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
                645                 650                 655     


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
            660                 665                 670         


Ser Pro Gly Lys 
        675     


<210>  35
<211>  468
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer (2 stalk regions) amino acid 
       sequence with signal sequence (aa 1-27)

<400>  35

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Asn Pro Gln Ile Ala 
            20                  25                  30          


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
        35                  40                  45              


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
    50                  55                  60                  


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
65                  70                  75                  80  


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
                85                  90                  95      


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
            100                 105                 110         


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
        115                 120                 125             


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
    130                 135                 140                 


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
145                 150                 155                 160 


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln 
                165                 170                 175     


Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
            180                 185                 190         


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
        195                 200                 205             


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
    210                 215                 220                 


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
225                 230                 235                 240 


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
                245                 250                 255     


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
            260                 265                 270         


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
        275                 280                 285             


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
    290                 295                 300                 


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met 
305                 310                 315                 320 


Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu 
                325                 330                 335     


Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr 
            340                 345                 350         


Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu 
        355                 360                 365             


Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe 
    370                 375                 380                 


Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu 
385                 390                 395                 400 


Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala 
                405                 410                 415     


Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu 
            420                 425                 430         


Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val 
        435                 440                 445             


Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly 
    450                 455                 460                 


Leu Leu Lys Leu 
465             


<210>  36
<211>  441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer (2 stalk regions) amino acid 
       sequence without signal sequence

<400>  36

Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
1               5                   10                  15      


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
            20                  25                  30          


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
        35                  40                  45              


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
    50                  55                  60                  


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
65                  70                  75                  80  


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
                85                  90                  95      


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
            100                 105                 110         


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
        115                 120                 125             


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met 
    130                 135                 140                 


Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu 
145                 150                 155                 160 


Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr 
                165                 170                 175     


Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu 
            180                 185                 190         


Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe 
        195                 200                 205             


Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu 
    210                 215                 220                 


Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala 
225                 230                 235                 240 


Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu 
                245                 250                 255     


Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val 
            260                 265                 270         


Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly 
        275                 280                 285             


Leu Leu Lys Leu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala 
    290                 295                 300                 


His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp 
305                 310                 315                 320 


Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu 
                325                 330                 335     


Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr 
            340                 345                 350         


Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro 
        355                 360                 365             


Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile 
    370                 375                 380                 


Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln 
385                 390                 395                 400 


Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser 
                405                 410                 415     


Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly 
            420                 425                 430         


Phe Thr Ser Phe Gly Leu Leu Lys Leu 
        435                 440     


<210>  37
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD40L anchor region and extracellular domain amino acid 
       sequence

<400>  37

Ile Phe Met Tyr Leu Leu Thr Val Phe Leu Ile Thr Gln Met Ile Gly 
1               5                   10                  15      


Ser Ala Leu Phe Ala Val Tyr Leu His Arg Arg Leu Asp Lys Ile Glu 
            20                  25                  30          


Asp Glu Arg Asn Leu His Glu Asp Phe Val Phe Met Lys Thr Ile Gln 
        35                  40                  45              


Arg Cys Asn Thr Gly Glu Arg Ser Leu Ser Leu Leu Asn Cys Glu Glu 
    50                  55                  60                  


Ile Lys Ser Gln Phe Glu Gly Phe Val Lys Asp Ile Met Leu Asn Lys 
65                  70                  75                  80  


Glu Glu Thr Lys Lys Glu Asn Ser Phe Glu Met Gln Lys Gly Asp Gln 
                85                  90                  95      


Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
            100                 105                 110         


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
        115                 120                 125             


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
    130                 135                 140                 


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
145                 150                 155                 160 


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
                165                 170                 175     


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
            180                 185                 190         


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
        195                 200                 205             


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
    210                 215                 220                 


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
225                 230                 235                 


<210>  38
<211>  1347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer nucleotide sequence without 
       signal sequence

<400>  38
atgcaaaaag gggatcagaa tcctcaaatt gcagcacatg tcatatctga ggccagctca       60

aaaacaacat ctgtgctcca gtgggctgaa aaaggatact acaccatgag caacaacctc      120

gttaccctgg aaaatgggaa acagctgacc gttaaaagac aaggactcta ttatatctat      180

gcccaagtca ccttctgttc caatcgggaa gcatcttcac aagctccatt tatcgccagc      240

ctctgcctca agtcccccgg tcggttcgag agaatcctcc tcagagctgc aaatacccac      300

tcttccgcca aaccttgcgg gcaacaatcc attcacctcg gaggagtttt tgaactgcaa      360

ccaggggctt ctgtgtttgt caatgtgact gatccaagcc aagtgtctca tggaactggc      420

ttcacttcct ttggcctcct caaactcatg cagaaagggg accaaaaccc ccagatagcc      480

gctcacgtta tttccgaagc aagctcaaaa acaacatctg tgctccagtg ggctgaaaaa      540

ggatactaca ccatgagcaa caacctcgtt accctggaga acggaaagca actcactgtg      600

aagcggcagg ggctgtacta catatacgca caagtgactt tttgcagcaa cagggaggca      660

tcctctcagg cccctttcat tgccagcctc tgcctgaagt cccccggtag attcgagaga      720

atcctcctca gagctgcaaa tacccactcc tccgcaaaac cctgtggcca gcagagcatc      780

catctgggcg gcgtgttcga gctccagcct ggggcctccg tcttcgtgaa cgtcaccgac      840

ccttcccaag tcagccacgg cactggcttc acatcctttg gcctcctcaa actcatgcaa      900

aaaggcgatc agaatcctca aattgctgca catgtcattt ccgaagcctc atccaaaact      960

acctccgtcc tgcaatgggc cgagaagggg tattatacaa tgtcaaataa cctggttact     1020

ctggaaaacg gcaaacagct cactgttaag cgccaaggtc tctactatat atatgcacaa     1080

gttactttct gttcaaatcg cgaagcatca tcacaagcac catttatagc atcactctgt     1140

ctcaagtcac caggtcgctt tgaacgcata ctgctccgcg cagcaaatac tcactcatca     1200

gcaaaaccat gcggtcaaca atcaatacac ctcggtggtg tttttgagct ccaaccaggc     1260

gcttcagttt ttgttaatgt tactgatcca tcacaagttt cacatggtac aggtttcact     1320

tcatttggtc tgctcaaact ctaatag                                         1347


<210>  39
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 Fc region

<400>  39

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Lys 
225         


<210>  40
<211>  230
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 Fc region

<400>  40

Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu 
1               5                   10                  15      


Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
            20                  25                  30          


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
        35                  40                  45              


Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly 
    50                  55                  60                  


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn 
65                  70                  75                  80  


Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp 
                85                  90                  95      


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro 
            100                 105                 110         


Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu 
        115                 120                 125             


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn 
    130                 135                 140                 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
145                 150                 155                 160 


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
                165                 170                 175     


Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
            180                 185                 190         


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
        195                 200                 205             


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
    210                 215                 220                 


Ser Leu Ser Pro Gly Lys 
225                 230 


<210>  41
<211>  232
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 Fc region

<400>  41

Glu Pro Lys Ser Ser Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala 
1               5                   10                  15      


Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro 
            20                  25                  30          


Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val 
        35                  40                  45              


Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val 
    50                  55                  60                  


Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln 
65                  70                  75                  80  


Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln 
                85                  90                  95      


Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala 
            100                 105                 110         


Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro 
        115                 120                 125             


Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr 
    130                 135                 140                 


Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser 
145                 150                 155                 160 


Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr 
                165                 170                 175     


Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr 
            180                 185                 190         


Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe 
        195                 200                 205             


Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys 
    210                 215                 220                 


Ser Leu Ser Leu Ser Pro Gly Lys 
225                 230         


<210>  42
<211>  224
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 Fc region

<400>  42

Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser 
1               5                   10                  15      


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
            20                  25                  30          


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
        35                  40                  45              


Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
    50                  55                  60                  


Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser Thr Phe Arg Val Val 
65                  70                  75                  80  


Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
                85                  90                  95      


Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr 
            100                 105                 110         


Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
        115                 120                 125             


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
    130                 135                 140                 


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
145                 150                 155                 160 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp 
                165                 170                 175     


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
            180                 185                 190         


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
        195                 200                 205             


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 220                 


<210>  43
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 Fc region

<400>  43

Val Glu Arg Lys Ser Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro 
1               5                   10                  15      


Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn Ser 
65                  70                  75                  80  


Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala 
            100                 105                 110         


Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Pro Gly Lys 
225                 


<210>  44
<211>  224
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 Fc region variant (mutation for deglycosylation)

<400>  44

Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val Ala Gly Pro Ser 
1               5                   10                  15      


Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg 
            20                  25                  30          


Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro 
        35                  40                  45              


Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala 
    50                  55                  60                  


Lys Thr Lys Pro Arg Glu Glu Gln Phe Ala Ser Thr Phe Arg Val Val 
65                  70                  75                  80  


Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn Gly Lys Glu Tyr 
                85                  90                  95      


Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro Ile Glu Lys Thr 
            100                 105                 110         


Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu 
        115                 120                 125             


Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys 
    130                 135                 140                 


Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser 
145                 150                 155                 160 


Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Met Leu Asp 
                165                 170                 175     


Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser 
            180                 185                 190         


Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala 
        195                 200                 205             


Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 220                 


<210>  45
<211>  229
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 Fc region (mutation for deglycosylation)

<400>  45

Val Glu Arg Lys Ser Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro 
1               5                   10                  15      


Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr 
            20                  25                  30          


Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val 
        35                  40                  45              


Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val 
    50                  55                  60                  


Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Ala Ser 
65                  70                  75                  80  


Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu 
                85                  90                  95      


Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala 
            100                 105                 110         


Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro 
        115                 120                 125             


Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln 
    130                 135                 140                 


Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala 
145                 150                 155                 160 


Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr 
                165                 170                 175     


Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu 
            180                 185                 190         


Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser 
        195                 200                 205             


Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser 
    210                 215                 220                 


Leu Ser Pro Gly Lys 
225                 


<210>  46
<211>  330
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 Heavy chain constant region

<400>  46

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         


Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             


Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 


Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 


Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     


Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         


His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             


Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 


Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 


Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     


Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         


Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             


Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 


Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 


Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


<210>  47
<211>  326
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG2 Heavy chain constant region

<400>  47

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Asn Phe Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Thr Val Glu Arg Lys Cys Cys Val Glu Cys Pro Pro Cys Pro Ala Pro 
            100                 105                 110         


Pro Val Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp 
        115                 120                 125             


Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp 
    130                 135                 140                 


Val Ser His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly 
145                 150                 155                 160 


Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Asn 
                165                 170                 175     


Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp 
            180                 185                 190         


Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro 
        195                 200                 205             


Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu 
    210                 215                 220                 


Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn 
225                 230                 235                 240 


Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile 
                245                 250                 255     


Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr 
            260                 265                 270         


Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys 
        275                 280                 285             


Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys 
    290                 295                 300                 


Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu 
305                 310                 315                 320 


Ser Leu Ser Pro Gly Lys 
                325     


<210>  48
<211>  377
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG3 Heavy chain constant region

<400>  48

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Leu Lys Thr Pro Leu Gly Asp Thr Thr His Thr Cys Pro 
            100                 105                 110         


Arg Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg 
        115                 120                 125             


Cys Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys 
    130                 135                 140                 


Pro Glu Pro Lys Ser Cys Asp Thr Pro Pro Pro Cys Pro Arg Cys Pro 
145                 150                 155                 160 


Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys 
                165                 170                 175     


Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val 
            180                 185                 190         


Val Val Asp Val Ser His Glu Asp Pro Glu Val Gln Phe Lys Trp Tyr 
        195                 200                 205             


Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu 
    210                 215                 220                 


Gln Tyr Asn Ser Thr Phe Arg Val Val Ser Val Leu Thr Val Leu His 
225                 230                 235                 240 


Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys 
                245                 250                 255     


Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln 
            260                 265                 270         


Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met 
        275                 280                 285             


Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro 
    290                 295                 300                 


Ser Asp Ile Ala Val Glu Trp Glu Ser Ser Gly Gln Pro Glu Asn Asn 
305                 310                 315                 320 


Tyr Asn Thr Thr Pro Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu 
                325                 330                 335     


Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Ile 
            340                 345                 350         


Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn Arg Phe Thr Gln 
        355                 360                 365             


Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    370                 375         


<210>  49
<211>  327
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG4 Heavy chain constant region

<400>  49

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Cys Ser Arg 
1               5                   10                  15      


Ser Thr Ser Glu Ser Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Lys Thr 
65                  70                  75                  80  


Tyr Thr Cys Asn Val Asp His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Arg Val Glu Ser Lys Tyr Gly Pro Pro Cys Pro Ser Cys Pro Ala Pro 
            100                 105                 110         


Glu Phe Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys 
        115                 120                 125             


Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val 
    130                 135                 140                 


Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp 
145                 150                 155                 160 


Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe 
                165                 170                 175     


Asn Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp 
            180                 185                 190         


Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu 
        195                 200                 205             


Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg 
    210                 215                 220                 


Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys 
225                 230                 235                 240 


Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp 
                245                 250                 255     


Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys 
            260                 265                 270         


Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser 
        275                 280                 285             


Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser 
    290                 295                 300                 


Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser 
305                 310                 315                 320 


Leu Ser Leu Ser Leu Gly Lys 
                325         


<210>  50
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  50

Gly Gly Gly Ser Gly Gly Gly 
1               5           


<210>  51
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  51

Glu Ser Gly Gly Gly Gly Val Thr 
1               5               


<210>  52
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  52

Leu Glu Ser Gly Gly Gly Gly Val Thr 
1               5                   


<210>  53
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  53

Gly Arg Ala Gln Val Thr 
1               5       


<210>  54
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  54

Trp Arg Ala Gln Val Thr 
1               5       


<210>  55
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  55

Ala Arg Gly Arg Ala Gln Val Thr 
1               5               


<210>  56
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  FLAG Tag

<400>  56

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  57
<211>  701
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer-Fc (deglycosylated hIgG1) amino 
       acid sequence with signal sequence (aa 1-27)

<400>  57

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Met Gln Lys Gly Asp 
            20                  25                  30          


Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys 
        35                  40                  45              


Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser 
    50                  55                  60                  


Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg 
65                  70                  75                  80  


Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg 
                85                  90                  95      


Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser 
            100                 105                 110         


Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser 
        115                 120                 125             


Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe 
    130                 135                 140                 


Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser 
145                 150                 155                 160 


Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
                165                 170                 175     


Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
            180                 185                 190         


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
        195                 200                 205             


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
    210                 215                 220                 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
225                 230                 235                 240 


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
                245                 250                 255     


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
            260                 265                 270         


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
        275                 280                 285             


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
    290                 295                 300                 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
305                 310                 315                 320 


Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala 
                325                 330                 335     


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
            340                 345                 350         


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
        355                 360                 365             


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
    370                 375                 380                 


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
385                 390                 395                 400 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
                405                 410                 415     


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
            420                 425                 430         


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
        435                 440                 445             


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
    450                 455                 460                 


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Asp Lys Thr His Thr Cys 
465                 470                 475                 480 


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
                485                 490                 495     


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
            500                 505                 510         


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
        515                 520                 525             


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
    530                 535                 540                 


Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr Arg Val Val Ser Val Leu 
545                 550                 555                 560 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
                565                 570                 575     


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
            580                 585                 590         


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
        595                 600                 605             


Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
    610                 615                 620                 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
625                 630                 635                 640 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
                645                 650                 655     


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
            660                 665                 670         


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
        675                 680                 685             


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    690                 695                 700     


<210>  58
<211>  674
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain CD40L trimer-Fc (deglycosylated hIgG1) amino 
       acid sequence without signal sequence

<400>  58

Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln Asn Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
        195                 200                 205             


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
    210                 215                 220                 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
                245                 250                 255     


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
        275                 280                 285             


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met Gln Lys Gly Asp Gln 
    290                 295                 300                 


Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
305                 310                 315                 320 


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
                325                 330                 335     


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
            340                 345                 350         


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
    370                 375                 380                 


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
            420                 425                 430         


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Asp 
        435                 440                 445             


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
    450                 455                 460                 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
465                 470                 475                 480 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
                485                 490                 495     


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
            500                 505                 510         


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr Arg 
        515                 520                 525             


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
    530                 535                 540                 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
545                 550                 555                 560 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
                565                 570                 575     


Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
            580                 585                 590         


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
        595                 600                 605             


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
    610                 615                 620                 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
625                 630                 635                 640 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
                645                 650                 655     


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
            660                 665                 670         


Gly Lys 
        


<210>  59
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human integrin-binding region mutant CD40L Stalk Region - 
       Fragment 1

<400>  59

Met Gln Lys Ala Asp Gln 
1               5       


<210>  60
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human integrin-binding region mutant CD40L Stalk Region - 
       Fragment 2

<400>  60

Phe Glu Met Gln Lys Ala Asp Gln 
1               5               


<210>  61
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human integrin-binding region mutant CD40L Stalk Region - 
       Fragment 3

<400>  61

Glu Met Gln Lys Ala Asp Gln 
1               5           


<210>  62
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human integrin-binding region mutant CD40L Stalk Region - 
       Fragment 4

<400>  62

Gln Lys Ala Asp Gln 
1               5   


<210>  63
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human integrin-binding region mutant CD40L Stalk Region - 
       Fragment 5

<400>  63

Lys Ala Asp Gln 
1               


<210>  64
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human integrin-binding region mutant CD40L extracellular with 
       stalk fragment 1 (aa 113-261)

<400>  64

Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu 
145                 


<210>  65
<211>  151
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human integrin-binding region mutant CD40L extracellular domain 
       with stalk fragment 2 (aa 111-261)

<400>  65

Phe Glu Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala Ala His Val 
1               5                   10                  15      


Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu 
            20                  25                  30          


Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly 
        35                  40                  45              


Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln 
    50                  55                  60                  


Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile 
65                  70                  75                  80  


Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu 
                85                  90                  95      


Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser 
            100                 105                 110         


Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe 
        115                 120                 125             


Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr 
    130                 135                 140                 


Ser Phe Gly Leu Leu Lys Leu 
145                 150     


<210>  66
<211>  150
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human integrin-binding region mutant CD40L extracellular domain 
       with stalk fragment 3 (aa 112-261)

<400>  66

Glu Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile 
1               5                   10                  15      


Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys 
            20                  25                  30          


Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys 
        35                  40                  45              


Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val 
    50                  55                  60                  


Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala 
65                  70                  75                  80  


Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg 
                85                  90                  95      


Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile 
            100                 105                 110         


His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val 
        115                 120                 125             


Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser 
    130                 135                 140                 


Phe Gly Leu Leu Lys Leu 
145                 150 


<210>  67
<211>  148
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human integrin-binding region mutant CD40L extracellular domain 
       with stalk fragment 4 (aa 114-261)

<400>  67

Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu 
1               5                   10                  15      


Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr 
            20                  25                  30          


Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu 
        35                  40                  45              


Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe 
    50                  55                  60                  


Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu 
65                  70                  75                  80  


Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala 
                85                  90                  95      


Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu 
            100                 105                 110         


Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val 
        115                 120                 125             


Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly 
    130                 135                 140                 


Leu Leu Lys Leu 
145             


<210>  68
<211>  147
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human integrin-binding region mutant CD40L extracellular domain 
       with stalk fragment 5 (aa 115-261)

<400>  68

Lys Ala Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala 
1               5                   10                  15      


Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr 
            20                  25                  30          


Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr 
        35                  40                  45              


Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys 
    50                  55                  60                  


Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys 
65                  70                  75                  80  


Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn 
                85                  90                  95      


Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly 
            100                 105                 110         


Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr 
        115                 120                 125             


Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu 
    130                 135                 140                 


Leu Lys Leu 
145         


<210>  69
<211>  474
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain integrin-binding region mutant CD40L trimer 
       amino acid sequence with signal sequence (aa 1-27)

<400>  69

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Met Gln Lys Ala Asp 
            20                  25                  30          


Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys 
        35                  40                  45              


Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser 
    50                  55                  60                  


Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg 
65                  70                  75                  80  


Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg 
                85                  90                  95      


Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser 
            100                 105                 110         


Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser 
        115                 120                 125             


Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe 
    130                 135                 140                 


Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser 
145                 150                 155                 160 


Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
                165                 170                 175     


Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
            180                 185                 190         


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
        195                 200                 205             


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
    210                 215                 220                 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
225                 230                 235                 240 


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
                245                 250                 255     


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
            260                 265                 270         


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
        275                 280                 285             


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
    290                 295                 300                 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
305                 310                 315                 320 


Gly Leu Leu Lys Leu Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala 
                325                 330                 335     


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
            340                 345                 350         


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
        355                 360                 365             


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
    370                 375                 380                 


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
385                 390                 395                 400 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
                405                 410                 415     


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
            420                 425                 430         


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
        435                 440                 445             


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
    450                 455                 460                 


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
465                 470                 


<210>  70
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain integrin-binding region mutant CD40L trimer 
       amino acid sequence without signal sequence 
       
<400>  70

Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
        195                 200                 205             


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
    210                 215                 220                 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
                245                 250                 255     


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
        275                 280                 285             


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met Gln Lys Ala Asp Gln 
    290                 295                 300                 


Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
305                 310                 315                 320 


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
                325                 330                 335     


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
            340                 345                 350         


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
    370                 375                 380                 


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
            420                 425                 430         


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
        435                 440                 445         


<210>  71
<211>  701
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain integrin-binding region mutant CD40L trimer Fc
       (deglycosylated hIgG1) amino acid sequence with signal sequence 
       (aa 1-27)

<400>  71

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Met Gln Lys Ala Asp 
            20                  25                  30          


Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys 
        35                  40                  45              


Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser 
    50                  55                  60                  


Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg 
65                  70                  75                  80  


Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg 
                85                  90                  95      


Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser 
            100                 105                 110         


Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser 
        115                 120                 125             


Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe 
    130                 135                 140                 


Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser 
145                 150                 155                 160 


Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
                165                 170                 175     


Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
            180                 185                 190         


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
        195                 200                 205             


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
    210                 215                 220                 


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
225                 230                 235                 240 


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
                245                 250                 255     


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
            260                 265                 270         


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
        275                 280                 285             


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
    290                 295                 300                 


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
305                 310                 315                 320 


Gly Leu Leu Lys Leu Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala 
                325                 330                 335     


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
            340                 345                 350         


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
        355                 360                 365             


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
    370                 375                 380                 


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
385                 390                 395                 400 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
                405                 410                 415     


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
            420                 425                 430         


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
        435                 440                 445             


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
    450                 455                 460                 


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Asp Lys Thr His Thr Cys 
465                 470                 475                 480 


Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu 
                485                 490                 495     


Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu 
            500                 505                 510         


Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys 
        515                 520                 525             


Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys 
    530                 535                 540                 


Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr Arg Val Val Ser Val Leu 
545                 550                 555                 560 


Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys 
                565                 570                 575     


Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys 
            580                 585                 590         


Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser 
        595                 600                 605             


Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys 
    610                 615                 620                 


Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln 
625                 630                 635                 640 


Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly 
                645                 650                 655     


Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln 
            660                 665                 670         


Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn 
        675                 680                 685             


His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    690                 695                 700     


<210>  72
<211>  674
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain integrin-binding region mutant CD40L trimer Fc
       (deglycosylated hIgG1) amino acid sequence without signal 
       sequence

<400>  72

Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
        195                 200                 205             


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
    210                 215                 220                 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
                245                 250                 255     


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
        275                 280                 285             


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met Gln Lys Ala Asp Gln 
    290                 295                 300                 


Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
305                 310                 315                 320 


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
                325                 330                 335     


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
            340                 345                 350         


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
    370                 375                 380                 


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
            420                 425                 430         


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Asp 
        435                 440                 445             


Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
    450                 455                 460                 


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
465                 470                 475                 480 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
                485                 490                 495     


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
            500                 505                 510         


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Ala Ser Thr Tyr Arg 
        515                 520                 525             


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
    530                 535                 540                 


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
545                 550                 555                 560 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
                565                 570                 575     


Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu 
            580                 585                 590         


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
        595                 600                 605             


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
    610                 615                 620                 


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
625                 630                 635                 640 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
                645                 650                 655     


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
            660                 665                 670         


Gly Lys 
        


<210>  73
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse integrin-binding region mutant CD40L Stalk - Fragment 1

<400>  73

Met Gln Arg Ala Asp Glu 
1               5       


<210>  74
<211>  149
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse integrin-binding region mutant CD40L extracellular domain 
       with stalk fragment 1 (aa 112-260)

<400>  74

Met Gln Arg Ala Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser 
1               5                   10                  15      


Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly 
65                  70                  75                  80  


Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu 
145                 


<210>  75
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mouse single chain integrin-binding region mutant CD40L trimer 
       amino acid sequence without signal sequence

<400>  75

Met Gln Arg Ala Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser 
1               5                   10                  15      


Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly 
65                  70                  75                  80  


Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Arg Ala Asp Glu Asp Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val 
        195                 200                 205             


Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg 
    210                 215                 220                 


Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu 
                245                 250                 255     


Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile His Arg Val 
        275                 280                 285             


Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu Met Gln Arg Ala Asp Glu 
    290                 295                 300                 


Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn Ala 
305                 310                 315                 320 


Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser 
                325                 330                 335     


Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu 
            340                 345                 350         


Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser 
    370                 375                 380                 


Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln 
            420                 425                 430         


Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu 
        435                 440                 445         


<210>  76
<211>  700
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  340F7 Mouse single chain integrin-binding region mutant CD40L 
       trimer-Fc (deglycosylated mIgG2a) with predicted signal sequence 
       (aa 1-27)

<400>  76

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Met Gln Arg Ala Asp 
            20                  25                  30          


Glu Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn 
        35                  40                  45              


Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys 
    50                  55                  60                  


Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg 
65                  70                  75                  80  


Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg 
                85                  90                  95      


Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro 
            100                 105                 110         


Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser 
        115                 120                 125             


Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe 
    130                 135                 140                 


Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser 
145                 150                 155                 160 


Gln Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu 
                165                 170                 175     


Met Gln Arg Ala Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser 
            180                 185                 190         


Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly 
        195                 200                 205             


Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln 
    210                 215                 220                 


Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr 
225                 230                 235                 240 


Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly 
                245                 250                 255     


Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala 
            260                 265                 270         


Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His 
        275                 280                 285             


Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn 
    290                 295                 300                 


Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe 
305                 310                 315                 320 


Gly Leu Leu Lys Leu Met Gln Arg Ala Asp Glu Asp Pro Gln Ile Ala 
                325                 330                 335     


Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln 
            340                 345                 350         


Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu 
        355                 360                 365             


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val 
    370                 375                 380                 


Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg 
385                 390                 395                 400 


Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg 
                405                 410                 415     


Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu 
            420                 425                 430         


Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala 
        435                 440                 445             


Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile His Arg Val 
    450                 455                 460                 


Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu Asp Lys Thr His Thr Cys 
465                 470                 475                 480 


Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser Val Phe Ile Phe 
                485                 490                 495     


Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser Pro Ile Val 
            500                 505                 510         


Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln Ile 
        515                 520                 525             


Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln Thr Gln Thr 
    530                 535                 540                 


His Arg Glu Asp Tyr Ala Ser Thr Leu Arg Val Val Ser Ala Leu Pro 
545                 550                 555                 560 


Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys Val 
                565                 570                 575     


Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile Ser Lys Pro 
            580                 585                 590         


Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro Pro Pro Glu 
        595                 600                 605             


Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met Val Thr Asp 
    610                 615                 620                 


Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn Gly Lys Thr 
625                 630                 635                 640 


Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser Asp Gly Ser 
                645                 650                 655     


Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn Trp Val Glu 
            660                 665                 670         


Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu His Asn His 
        675                 680                 685             


His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys 
    690                 695                 700 


<210>  77
<211>  673
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  340F7 Mouse single chain integrin-binding region mutant CD40L 
       trimer-Fc (deglycosylated mIgG2a) without signal sequence

<400>  77

Met Gln Arg Ala Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser 
1               5                   10                  15      


Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly 
65                  70                  75                  80  


Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Arg Ala Asp Glu Asp Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val 
        195                 200                 205             


Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg 
    210                 215                 220                 


Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu 
                245                 250                 255     


Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile His Arg Val 
        275                 280                 285             


Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu Met Gln Arg Ala Asp Glu 
    290                 295                 300                 


Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn Ala 
305                 310                 315                 320 


Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser 
                325                 330                 335     


Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu 
            340                 345                 350         


Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser 
    370                 375                 380                 


Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln 
            420                 425                 430         


Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu Asp 
        435                 440                 445             


Lys Thr His Thr Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro 
    450                 455                 460                 


Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser 
465                 470                 475                 480 


Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp 
                485                 490                 495     


Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr 
            500                 505                 510         


Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Ala Ser Thr Leu Arg Val 
        515                 520                 525             


Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu 
    530                 535                 540                 


Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg 
545                 550                 555                 560 


Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val 
                565                 570                 575     


Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr 
            580                 585                 590         


Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr 
        595                 600                 605             


Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu 
    610                 615                 620                 


Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys 
625                 630                 635                 640 


Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu 
                645                 650                 655     


Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly 
            660                 665                 670         


Lys 
    


<210>  78
<211>  700
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  340F4 Mouse single chain CD40L trimer (N term)-Fc (C term) 
       (deglycosylated mIgG2a) with predicted signal sequence (aa 1-27)

<400>  78

Met Glu Trp Gly Tyr Leu Leu Glu Val Thr Ser Leu Leu Ala Ala Leu 
1               5                   10                  15      


Leu Leu Leu Gln Arg Ser Pro Ile Val His Ala Met Gln Arg Gly Asp 
            20                  25                  30          


Glu Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn 
        35                  40                  45              


Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys 
    50                  55                  60                  


Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg 
65                  70                  75                  80  


Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg 
                85                  90                  95      


Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro 
            100                 105                 110         


Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser 
        115                 120                 125             


Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe 
    130                 135                 140                 


Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser 
145                 150                 155                 160 


Gln Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu 
                165                 170                 175     


Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser 
            180                 185                 190         


Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly 
        195                 200                 205             


Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln 
    210                 215                 220                 


Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr 
225                 230                 235                 240 


Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly 
                245                 250                 255     


Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala 
            260                 265                 270         


Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His 
        275                 280                 285             


Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn 
    290                 295                 300                 


Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe 
305                 310                 315                 320 


Gly Leu Leu Lys Leu Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala 
                325                 330                 335     


Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln 
            340                 345                 350         


Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu 
        355                 360                 365             


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val 
    370                 375                 380                 


Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg 
385                 390                 395                 400 


Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg 
                405                 410                 415     


Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu 
            420                 425                 430         


Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala 
        435                 440                 445             


Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile His Arg Val 
    450                 455                 460                 


Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu Asp Lys Thr His Thr Cys 
465                 470                 475                 480 


Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro Ser Val Phe Ile Phe 
                485                 490                 495     


Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser Leu Ser Pro Ile Val 
            500                 505                 510         


Thr Cys Val Val Val Asp Val Ser Glu Asp Asp Pro Asp Val Gln Ile 
        515                 520                 525             


Ser Trp Phe Val Asn Asn Val Glu Val His Thr Ala Gln Thr Gln Thr 
    530                 535                 540                 


His Arg Glu Asp Tyr Ala Ser Thr Leu Arg Val Val Ser Ala Leu Pro 
545                 550                 555                 560 


Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu Phe Lys Cys Lys Val 
                565                 570                 575     


Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg Thr Ile Ser Lys Pro 
            580                 585                 590         


Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val Leu Pro Pro Pro Glu 
        595                 600                 605             


Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr Cys Met Val Thr Asp 
    610                 615                 620                 


Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr Asn Asn Gly Lys Thr 
625                 630                 635                 640 


Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu Asp Ser Asp Gly Ser 
                645                 650                 655     


Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys Lys Asn Trp Val Glu 
            660                 665                 670         


Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu Gly Leu His Asn His 
        675                 680                 685             


His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly Lys 
    690                 695                 700 


<210>  79
<211>  673
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  340F4 Mouse single chain CD40L trimer (N term)-Fc (C term) 
       (deglycosylated mIgG2a) without signal sequence

<400>  79

Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala Ala His Val Val Ser 
1               5                   10                  15      


Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln Trp Ala Lys Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg Pro Phe Ile Val Gly 
65                  70                  75                  80  


Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu Gln Gln Ser Val His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Glu Ala Ser Gln Val Ile His Arg Val Gly Phe Ser Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Arg Gly Asp Glu Asp Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Val Ser Glu Ala Asn Ser Asn Ala Ala Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser Asn Leu Val Met Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu Gly Leu Tyr Tyr Val 
        195                 200                 205             


Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu Pro Ser Ser Gln Arg 
    210                 215                 220                 


Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser Ser Gly Ser Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser Ser Gln Leu Cys Glu 
                245                 250                 255     


Gln Gln Ser Val His Leu Gly Gly Val Phe Glu Leu Gln Ala Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln Val Ile His Arg Val 
        275                 280                 285             


Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu Met Gln Arg Gly Asp Glu 
    290                 295                 300                 


Asp Pro Gln Ile Ala Ala His Val Val Ser Glu Ala Asn Ser Asn Ala 
305                 310                 315                 320 


Ala Ser Val Leu Gln Trp Ala Lys Lys Gly Tyr Tyr Thr Met Lys Ser 
                325                 330                 335     


Asn Leu Val Met Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Glu 
            340                 345                 350         


Gly Leu Tyr Tyr Val Tyr Thr Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Pro Ser Ser Gln Arg Pro Phe Ile Val Gly Leu Trp Leu Lys Pro Ser 
    370                 375                 380                 


Ser Gly Ser Glu Arg Ile Leu Leu Lys Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ser Gln Leu Cys Glu Gln Gln Ser Val His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Ala Gly Ala Ser Val Phe Val Asn Val Thr Glu Ala Ser Gln 
            420                 425                 430         


Val Ile His Arg Val Gly Phe Ser Ser Phe Gly Leu Leu Lys Leu Asp 
        435                 440                 445             


Lys Thr His Thr Cys Lys Cys Pro Ala Pro Asn Leu Leu Gly Gly Pro 
    450                 455                 460                 


Ser Val Phe Ile Phe Pro Pro Lys Ile Lys Asp Val Leu Met Ile Ser 
465                 470                 475                 480 


Leu Ser Pro Ile Val Thr Cys Val Val Val Asp Val Ser Glu Asp Asp 
                485                 490                 495     


Pro Asp Val Gln Ile Ser Trp Phe Val Asn Asn Val Glu Val His Thr 
            500                 505                 510         


Ala Gln Thr Gln Thr His Arg Glu Asp Tyr Ala Ser Thr Leu Arg Val 
        515                 520                 525             


Val Ser Ala Leu Pro Ile Gln His Gln Asp Trp Met Ser Gly Lys Glu 
    530                 535                 540                 


Phe Lys Cys Lys Val Asn Asn Lys Asp Leu Pro Ala Pro Ile Glu Arg 
545                 550                 555                 560 


Thr Ile Ser Lys Pro Lys Gly Ser Val Arg Ala Pro Gln Val Tyr Val 
                565                 570                 575     


Leu Pro Pro Pro Glu Glu Glu Met Thr Lys Lys Gln Val Thr Leu Thr 
            580                 585                 590         


Cys Met Val Thr Asp Phe Met Pro Glu Asp Ile Tyr Val Glu Trp Thr 
        595                 600                 605             


Asn Asn Gly Lys Thr Glu Leu Asn Tyr Lys Asn Thr Glu Pro Val Leu 
    610                 615                 620                 


Asp Ser Asp Gly Ser Tyr Phe Met Tyr Ser Lys Leu Arg Val Glu Lys 
625                 630                 635                 640 


Lys Asn Trp Val Glu Arg Asn Ser Tyr Ser Cys Ser Val Val His Glu 
                645                 650                 655     


Gly Leu His Asn His His Thr Thr Lys Ser Phe Ser Arg Thr Pro Gly 
            660                 665                 670         


Lys 
    


<210>  80
<211>  447
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain integrin-binding region mutant CD40L trimer (2
       stalk regions) amino acid sequence without signal sequence

<400>  80

Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
        195                 200                 205             


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
    210                 215                 220                 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
                245                 250                 255     


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
        275                 280                 285             


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met Gln Lys Ala Asp Gln 
    290                 295                 300                 


Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
305                 310                 315                 320 


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
                325                 330                 335     


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
            340                 345                 350         


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
    370                 375                 380                 


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
            420                 425                 430         


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu 
        435                 440                 445         


<210>  81
<211>  676
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human single chain integrin-binding region mutant CD40L trimer Fc
       (deglycosylated hIgG2) amino acid sequence without signal 
       sequence

<400>  81

Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala Ala His Val Ile Ser 
1               5                   10                  15      


Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln Trp Ala Glu Lys Gly 
            20                  25                  30          


Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu Glu Asn Gly Lys Gln 
        35                  40                  45              


Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr 
    50                  55                  60                  


Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser 
65                  70                  75                  80  


Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala 
                85                  90                  95      


Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly Gln Gln Ser Ile His 
            100                 105                 110         


Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala Ser Val Phe Val Asn 
        115                 120                 125             


Val Thr Asp Pro Ser Gln Val Ser His Gly Thr Gly Phe Thr Ser Phe 
    130                 135                 140                 


Gly Leu Leu Lys Leu Met Gln Lys Ala Asp Gln Asn Pro Gln Ile Ala 
145                 150                 155                 160 


Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr Thr Ser Val Leu Gln 
                165                 170                 175     


Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn Asn Leu Val Thr Leu 
            180                 185                 190         


Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln Gly Leu Tyr Tyr Ile 
        195                 200                 205             


Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu Ala Ser Ser Gln Ala 
    210                 215                 220                 


Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro Gly Arg Phe Glu Arg 
225                 230                 235                 240 


Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser Ala Lys Pro Cys Gly 
                245                 250                 255     


Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu Leu Gln Pro Gly Ala 
            260                 265                 270         


Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln Val Ser His Gly Thr 
        275                 280                 285             


Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Met Gln Lys Ala Asp Gln 
    290                 295                 300                 


Asn Pro Gln Ile Ala Ala His Val Ile Ser Glu Ala Ser Ser Lys Thr 
305                 310                 315                 320 


Thr Ser Val Leu Gln Trp Ala Glu Lys Gly Tyr Tyr Thr Met Ser Asn 
                325                 330                 335     


Asn Leu Val Thr Leu Glu Asn Gly Lys Gln Leu Thr Val Lys Arg Gln 
            340                 345                 350         


Gly Leu Tyr Tyr Ile Tyr Ala Gln Val Thr Phe Cys Ser Asn Arg Glu 
        355                 360                 365             


Ala Ser Ser Gln Ala Pro Phe Ile Ala Ser Leu Cys Leu Lys Ser Pro 
    370                 375                 380                 


Gly Arg Phe Glu Arg Ile Leu Leu Arg Ala Ala Asn Thr His Ser Ser 
385                 390                 395                 400 


Ala Lys Pro Cys Gly Gln Gln Ser Ile His Leu Gly Gly Val Phe Glu 
                405                 410                 415     


Leu Gln Pro Gly Ala Ser Val Phe Val Asn Val Thr Asp Pro Ser Gln 
            420                 425                 430         


Val Ser His Gly Thr Gly Phe Thr Ser Phe Gly Leu Leu Lys Leu Val 
        435                 440                 445             


Glu Arg Lys Ser Cys Val Glu Cys Pro Pro Cys Pro Ala Pro Pro Val 
    450                 455                 460                 


Ala Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu 
465                 470                 475                 480 


Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser 
                485                 490                 495     


His Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly Val Glu 
            500                 505                 510         


Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Ala Ser Thr 
        515                 520                 525             


Phe Arg Val Val Ser Val Leu Thr Val Val His Gln Asp Trp Leu Asn 
    530                 535                 540                 


Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro Ala Pro 
545                 550                 555                 560 


Ile Glu Lys Thr Ile Ser Lys Thr Lys Gly Gln Pro Arg Glu Pro Gln 
                565                 570                 575     


Val Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val 
            580                 585                 590         


Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val 
        595                 600                 605             


Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro 
    610                 615                 620                 


Pro Met Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr 
625                 630                 635                 640 


Val Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val 
                645                 650                 655     


Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu 
            660                 665                 670         


Ser Pro Gly Lys 
        675     


<210>  82
<211>  227
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human IgG1 Fc region variant (mutation for deglycosylation)

<400>  82

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Lys 
225         


