                         SEQUENCE LISTING

<110>  Conagen Inc.
 
<120>  BIOSYNTHESIS OF ERIODICTYOL FROM ENGINEERED MICROBES

<130>  CCN-1203WO

<140>  PCT/US2018/041745
<141>  2018-07-12

<150>  62/542843
<151>  2017-08-09

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1539
<212>  DNA
<213>  Saccharothrix espanaensis

<400>  1
atgacgatta cctctccggc cccggctggt cgcctgaaca atgtgcgtcc gatgacgggt       60

gaagaatacc tggaatccct gcgtgacggt cgtgaagtgt atatttacgg cgaacgcgtc      120

gatgacgtga ccacgcatct ggcgttccgc aacagcgttc gttctatcgc ccgcctgtat      180

gatgtcctgc acgatccggc ctccgaaggt gttctgcgcg tcccgaccga taccggtaat      240

ggtggtttta cccatccgtt tttcaaaacg gcgcgtagct ctgaagacct ggtggcggcc      300

cgtgaagcca ttgtcggttg gcaacgcctg gtgtatggct ggatgggtcg taccccggat      360

tacaaggcag cgtttttcgg tacgctggac gctaacgcgg aattttatgg cccgttcgaa      420

gccaatgcac gtcgctggta tcgtgatgca caggaacgcg ttctgtactt caaccatgct      480

atcgtgcatc cgccggtcga tcgtgaccgt ccggctgatc gtaccgccga catttgcgtc      540

catgtggaag aagaaacgga ttcaggcctg atcgtgtcgg gtgccaaagt ggttgcaacc      600

ggttctgcta tgacgaacgc gaatctgatt gcccactatg gtctgccggt tcgcgataaa      660

aagtttggcc tggtgttcac cgttccgatg aacagtccgg gtctgaaact gatctgtcgt      720

acctcctatg aactgatggt ggccacgcag ggctcaccgt ttgattaccc gctgagttcc      780

cgcctggatg aaaatgacag cattatgatc tttgatcgtg ttctggtccc gtgggaaaac      840

gttttcatgt acgacgcagg cgcggccaat agctttgcta ccggctctgg tttcctggaa      900

cgctttacct ttcatggctg cacgcgtctg gcagtgaaac tggattttat tgcaggctgt      960

gttatgaagg ctgtggaagt taccggcacc acgcacttcc gcggtgttca ggcgcaagtc     1020

ggcgaagtgc tgaactggcg tgatgtcttt tggggtctgt cggacgctat ggcgaaaagt     1080

ccgaacagct gggtgggcgg tagcgttcag ccgaacctga attatggcct ggcctaccgc     1140

acctttatgg gcgtgggtta tccgcgtatt aaagaaatta tccagcaaac gctgggctct     1200

ggtctgatct acctgaactc atcggcagct gattggaaga atccggacgt tcgcccgtat     1260

ctggatcgtt acctgcgcgg cagtcgtggt attcaggcaa tcgatcgtgt caaactgctg     1320

aagctgctgt gggacgcggt gggcaccgaa tttgccggtc gtcatgaact gtatgaacgc     1380

aactacggcg gtgatcacga aggcattcgt gtgcagaccc tgcaagccta tcaggcaaat     1440

ggtcaagcgg cggcactgaa aggctttgcg gaacagtgca tgagcgaata cgacctggat     1500

ggctggaccc gcccggacct gattaacccg ggcacctga                            1539


<210>  2
<211>  512
<212>  PRT
<213>  Saccharothrix espanaensis

<400>  2

Met Thr Ile Thr Ser Pro Ala Pro Ala Gly Arg Leu Asn Asn Val Arg 
1               5                   10                  15      


Pro Met Thr Gly Glu Glu Tyr Leu Glu Ser Leu Arg Asp Gly Arg Glu 
            20                  25                  30          


Val Tyr Ile Tyr Gly Glu Arg Val Asp Asp Val Thr Thr His Leu Ala 
        35                  40                  45              


Phe Arg Asn Ser Val Arg Ser Ile Ala Arg Leu Tyr Asp Val Leu His 
    50                  55                  60                  


Asp Pro Ala Ser Glu Gly Val Leu Arg Val Pro Thr Asp Thr Gly Asn 
65                  70                  75                  80  


Gly Gly Phe Thr His Pro Phe Phe Lys Thr Ala Arg Ser Ser Glu Asp 
                85                  90                  95      


Leu Val Ala Ala Arg Glu Ala Ile Val Gly Trp Gln Arg Leu Val Tyr 
            100                 105                 110         


Gly Trp Met Gly Arg Thr Pro Asp Tyr Lys Ala Ala Phe Phe Gly Thr 
        115                 120                 125             


Leu Asp Ala Asn Ala Glu Phe Tyr Gly Pro Phe Glu Ala Asn Ala Arg 
    130                 135                 140                 


Arg Trp Tyr Arg Asp Ala Gln Glu Arg Val Leu Tyr Phe Asn His Ala 
145                 150                 155                 160 


Ile Val His Pro Pro Val Asp Arg Asp Arg Pro Ala Asp Arg Thr Ala 
                165                 170                 175     


Asp Ile Cys Val His Val Glu Glu Glu Thr Asp Ser Gly Leu Ile Val 
            180                 185                 190         


Ser Gly Ala Lys Val Val Ala Thr Gly Ser Ala Met Thr Asn Ala Asn 
        195                 200                 205             


Leu Ile Ala His Tyr Gly Leu Pro Val Arg Asp Lys Lys Phe Gly Leu 
    210                 215                 220                 


Val Phe Thr Val Pro Met Asn Ser Pro Gly Leu Lys Leu Ile Cys Arg 
225                 230                 235                 240 


Thr Ser Tyr Glu Leu Met Val Ala Thr Gln Gly Ser Pro Phe Asp Tyr 
                245                 250                 255     


Pro Leu Ser Ser Arg Leu Asp Glu Asn Asp Ser Ile Met Ile Phe Asp 
            260                 265                 270         


Arg Val Leu Val Pro Trp Glu Asn Val Phe Met Tyr Asp Ala Gly Ala 
        275                 280                 285             


Ala Asn Ser Phe Ala Thr Gly Ser Gly Phe Leu Glu Arg Phe Thr Phe 
    290                 295                 300                 


His Gly Cys Thr Arg Leu Ala Val Lys Leu Asp Phe Ile Ala Gly Cys 
305                 310                 315                 320 


Val Met Lys Ala Val Glu Val Thr Gly Thr Thr His Phe Arg Gly Val 
                325                 330                 335     


Gln Ala Gln Val Gly Glu Val Leu Asn Trp Arg Asp Val Phe Trp Gly 
            340                 345                 350         


Leu Ser Asp Ala Met Ala Lys Ser Pro Asn Ser Trp Val Gly Gly Ser 
        355                 360                 365             


Val Gln Pro Asn Leu Asn Tyr Gly Leu Ala Tyr Arg Thr Phe Met Gly 
    370                 375                 380                 


Val Gly Tyr Pro Arg Ile Lys Glu Ile Ile Gln Gln Thr Leu Gly Ser 
385                 390                 395                 400 


Gly Leu Ile Tyr Leu Asn Ser Ser Ala Ala Asp Trp Lys Asn Pro Asp 
                405                 410                 415     


Val Arg Pro Tyr Leu Asp Arg Tyr Leu Arg Gly Ser Arg Gly Ile Gln 
            420                 425                 430         


Ala Ile Asp Arg Val Lys Leu Leu Lys Leu Leu Trp Asp Ala Val Gly 
        435                 440                 445             


Thr Glu Phe Ala Gly Arg His Glu Leu Tyr Glu Arg Asn Tyr Gly Gly 
    450                 455                 460                 


Asp His Glu Gly Ile Arg Val Gln Thr Leu Gln Ala Tyr Gln Ala Asn 
465                 470                 475                 480 


Gly Gln Ala Ala Ala Leu Lys Gly Phe Ala Glu Gln Cys Met Ser Glu 
                485                 490                 495     


Tyr Asp Leu Asp Gly Trp Thr Arg Pro Asp Leu Ile Asn Pro Gly Thr 
            500                 505                 510         


<210>  3
<211>  510
<212>  DNA
<213>  Saccharothrix espanaensis

<400>  3
atgatgaccg tttatgatag cgcactgaca atggaagaaa ccaccctgcg tgatgcaatg       60

agccgttttg caaccggtgt tagcgttgtt accgttggtg gtgaacatac acatggtatg      120

accgcaaatg cctttacctg tgttagcctg gatccgcctc tggttctgtg ttgtgttgca      180

cgtaaagcaa ccatgcatgc agcaattgaa ggtgcacgtc gttttgcagt tagcgttatg      240

ggtggtgatc aagaacgtac cgcacgttat tttgcagata aacgtcgtcc gcgtggtcgt      300

gcacagtttg atgttgttga ttggcagcct ggtccgcata caggtgcacc gctgctgagc      360

ggtgcgctgg catggctgga atgtgaagtt gcacagtggc atgaaggtgg cgatcatacc      420

atttttctgg gtcgtgttct gggttgtcgt cgtggtccgg atagtccggc actgctgttt      480

tatggtagcg attttcatca gatccgctaa                                       510


<210>  4
<211>  169
<212>  PRT
<213>  Saccharothrix espanaensis

<400>  4

Met Met Thr Val Tyr Asp Ser Ala Leu Thr Met Glu Glu Thr Thr Leu 
1               5                   10                  15      


Arg Asp Ala Met Ser Arg Phe Ala Thr Gly Val Ser Val Val Thr Val 
            20                  25                  30          


Gly Gly Glu His Thr His Gly Met Thr Ala Asn Ala Phe Thr Cys Val 
        35                  40                  45              


Ser Leu Asp Pro Pro Leu Val Leu Cys Cys Val Ala Arg Lys Ala Thr 
    50                  55                  60                  


Met His Ala Ala Ile Glu Gly Ala Arg Arg Phe Ala Val Ser Val Met 
65                  70                  75                  80  


Gly Gly Asp Gln Glu Arg Thr Ala Arg Tyr Phe Ala Asp Lys Arg Arg 
                85                  90                  95      


Pro Arg Gly Arg Ala Gln Phe Asp Val Val Asp Trp Gln Pro Gly Pro 
            100                 105                 110         


His Thr Gly Ala Pro Leu Leu Ser Gly Ala Leu Ala Trp Leu Glu Cys 
        115                 120                 125             


Glu Val Ala Gln Trp His Glu Gly Gly Asp His Thr Ile Phe Leu Gly 
    130                 135                 140                 


Arg Val Leu Gly Cys Arg Arg Gly Pro Asp Ser Pro Ala Leu Leu Phe 
145                 150                 155                 160 


Tyr Gly Ser Asp Phe His Gln Ile Arg 
                165                 


<210>  5
<211>  561
<212>  DNA
<213>  Pseudomonas fluorescens

<400>  5
atgaatgcag caaccgaaac caaagttcat gatctgctgg atgccgaagg tcgtgatgtt       60

cgtgatgcac gtgaactgcg taatgttctg ggtcagtttg caaccggtgt taccgttatt      120

accacccgta ccgcagatgg tcgtaatgtt ggtgtgaccg caaatagctt tagcagcctg      180

agcctgagtc cggcactggt tctgtggtca ctggcacgta ccgcaccgag cctgaaagtt      240

ttttgtagcg caagccattt tgccattaat gtgctgggtg cacatcagct gcatctgagc      300

gaacagtttg cacgtgccgc agcagataaa tttgccggtg ttgcacatag ttatggtaaa      360

gcgggtgcac cggttctgga tgatgttgtt gcagttctgg tttgccgtaa tgttacccag      420

tatgaaggtg gtgatcatct gatttttatc ggcgaaattg agcagtatcg ttatagcggt      480

gcagaaccgc tggtttttca tgcaggtcag tatcgtggtc tgggtagcaa tcgtgcagaa      540

agcgttctga aacatgaata a                                                561


<210>  6
<211>  186
<212>  PRT
<213>  Pseudomonas fluorescens

<400>  6

Met Asn Ala Ala Thr Glu Thr Lys Val His Asp Leu Leu Asp Ala Glu 
1               5                   10                  15      


Gly Arg Asp Val Arg Asp Ala Arg Glu Leu Arg Asn Val Leu Gly Gln 
            20                  25                  30          


Phe Ala Thr Gly Val Thr Val Ile Thr Thr Arg Thr Ala Asp Gly Arg 
        35                  40                  45              


Asn Val Gly Val Thr Ala Asn Ser Phe Ser Ser Leu Ser Leu Ser Pro 
    50                  55                  60                  


Ala Leu Val Leu Trp Ser Leu Ala Arg Thr Ala Pro Ser Leu Lys Val 
65                  70                  75                  80  


Phe Cys Ser Ala Ser His Phe Ala Ile Asn Val Leu Gly Ala His Gln 
                85                  90                  95      


Leu His Leu Ser Glu Gln Phe Ala Arg Ala Ala Ala Asp Lys Phe Ala 
            100                 105                 110         


Gly Val Ala His Ser Tyr Gly Lys Ala Gly Ala Pro Val Leu Asp Asp 
        115                 120                 125             


Val Val Ala Val Leu Val Cys Arg Asn Val Thr Gln Tyr Glu Gly Gly 
    130                 135                 140                 


Asp His Leu Ile Phe Ile Gly Glu Ile Glu Gln Tyr Arg Tyr Ser Gly 
145                 150                 155                 160 


Ala Glu Pro Leu Val Phe His Ala Gly Gln Tyr Arg Gly Leu Gly Ser 
                165                 170                 175     


Asn Arg Ala Glu Ser Val Leu Lys His Glu 
            180                 185     


<210>  7
<211>  513
<212>  DNA
<213>  Escherichia coli

<400>  7
atgcaattag atgaacaacg cctgcgcttt cgtgacgcaa tggccagcct gtcggcagcg       60

gtaaatatta tcaccaccga gggcgacgcc ggacaatgcg ggattacggc aacggccgtc      120

tgctcggtca cggatacacc accatcgctg atggtgtgca ttaacgccaa cagtgcgatg      180

aacccggttt ttcagggcaa cggtaagttg tgcgtcaacg tcctcaacca tgagcaggaa      240

ctgatggcac gccacttcgc gggcatgaca ggcatggcga tggaagagcg ttttagcctc      300

tcatgctggc aaaaaggtcc gctggcgcag ccggtgctaa aaggttcgct ggccagtctt      360

gaaggtgaga tccgcgatgt gcaggcaatt ggcacacatc tggtgtatct ggtggagatt      420

aaaaacatca tcctcagtgc agaaggtcac ggacttatct actttaaacg ccgtttccat      480

ccggtgatgc tggaaatgga agctgcgatt taa                                   513


<210>  8
<211>  170
<212>  PRT
<213>  Escherichia coli

<400>  8

Met Gln Leu Asp Glu Gln Arg Leu Arg Phe Arg Asp Ala Met Ala Ser 
1               5                   10                  15      


Leu Ser Ala Ala Val Asn Ile Ile Thr Thr Glu Gly Asp Ala Gly Gln 
            20                  25                  30          


Cys Gly Ile Thr Ala Thr Ala Val Cys Ser Val Thr Asp Thr Pro Pro 
        35                  40                  45              


Ser Leu Met Val Cys Ile Asn Ala Asn Ser Ala Met Asn Pro Val Phe 
    50                  55                  60                  


Gln Gly Asn Gly Lys Leu Cys Val Asn Val Leu Asn His Glu Gln Glu 
65                  70                  75                  80  


Leu Met Ala Arg His Phe Ala Gly Met Thr Gly Met Ala Met Glu Glu 
                85                  90                  95      


Arg Phe Ser Leu Ser Cys Trp Gln Lys Gly Pro Leu Ala Gln Pro Val 
            100                 105                 110         


Leu Lys Gly Ser Leu Ala Ser Leu Glu Gly Glu Ile Arg Asp Val Gln 
        115                 120                 125             


Ala Ile Gly Thr His Leu Val Tyr Leu Val Glu Ile Lys Asn Ile Ile 
    130                 135                 140                 


Leu Ser Ala Glu Gly His Gly Leu Ile Tyr Phe Lys Arg Arg Phe His 
145                 150                 155                 160 


Pro Val Met Leu Glu Met Glu Ala Ala Ile 
                165                 170 


