               SEQUENCE LISTING

<110> ISTITUTO ZOOPROFILATTICO SPERIMENTALE UMBRIA E MARCHE

<120> VP-60 RECOMBINANT PROTEIN AND RELATIVE METHOD OF PRODUCTION AND PURIFICATION

<130> EBHS

<150> IT102017000091210
<151> 2017-08-07

<160> 6

<170> BiSSAP 1.3.6

<210> 1
<211> 584
<212> PRT
<213> Baculoviridae


<220> 
<223> E-VP60-His6

<400> 1
Met Glu Gly Lys Pro Arg Ala Asp Ala Pro Gly Thr Ala Thr Thr Ala 
1               5                   10                  15      
Ser Val Pro Gly Thr Thr Thr Asp Gly Met Asp Pro Gly Val Val Ala 
            20                  25                  30          
Ser Thr Asp Val Val Thr Ala Asp Asn Val Ala Ala Ser Val Ala Thr 
        35                  40                  45              
Ala Gly Ile Gly Gly Pro Pro Gln Gln Val Ser Pro Gln Glu Ser Trp 
    50                  55                  60                  
Arg Ala Asn Phe Phe Tyr Asn Asp Val Phe Thr Trp Ser Val Thr Asp 
65                  70                  75                  80  
Ala Pro Gly Ser Ile Leu Tyr Thr Val Gln His Ser Pro Gln Asn Asn 
                85                  90                  95      
Pro Phe Thr Gln Val Leu Ser Gln Met Tyr Ala Gly Trp Ala Gly Gly 
            100                 105                 110         
Met Gln Phe Arg Phe Ile Val Ala Gly Ser Gly Ile Phe Gly Gly Arg 
        115                 120                 125             
Leu Val Cys Ala Ile Ile Pro Pro Gly Ile Gln Ile Gln Pro Gly Leu 
    130                 135                 140                 
Glu Val Arg Gln Phe Pro His Val Val Ile Asp Ala Arg Ser Leu Glu 
145                 150                 155                 160 
Pro Val Thr Ile Thr Met Pro Asp Leu Arg Pro Glu Met Tyr His Pro 
                165                 170                 175     
Thr Gly Asn Pro Gly Leu Val Pro Thr Leu Val Val Ser Val Tyr Asn 
            180                 185                 190         
Asn Leu Ile Asn Pro Phe Gly Gly Thr Thr Ser Ala Ile Gln Val Thr 
        195                 200                 205             
Val Glu Thr Arg Pro Ser Glu Asp Phe Glu Phe Val Leu Ile Arg Ala 
    210                 215                 220                 
Pro Ser Ser Lys Thr Val Asp Ser Val Asn Pro Ser Trp Leu Leu Thr 
225                 230                 235                 240 
Thr Pro Val Leu Thr Gly Ala Gly Ser Asp Asn Arg Trp Gly Ala Pro 
                245                 250                 255     
Ile Val Gly Leu Gln Pro Val Pro Gly Gly Phe Ser Thr Ser Asn Arg 
            260                 265                 270         
His Trp Asn Met Asn Gly Glu Thr Tyr Gly Trp Ser Ser Pro Arg Phe 
        275                 280                 285             
Asp Asp Ile Asp His Pro Ser Gly Asn Val Ser Tyr Pro Thr Gly Ser 
    290                 295                 300                 
Ala Thr Asn Thr Ile Glu Thr Trp Tyr Ala Asn Ala Gly Thr Ala Thr 
305                 310                 315                 320 
Thr Asn Pro Ile Ser Asn Val Ala Pro Asp Gly Phe Pro Asp Met Gly 
                325                 330                 335     
Ala Ile Pro Phe Ser Gly Ser Thr Ile Pro Thr Gly Ala Trp Val Gly 
            340                 345                 350         
Phe Gly Gln Val Trp Asn Ala Ser Asn Gly Thr Pro Tyr Val Gly Thr 
        355                 360                 365             
Val Gln Ala Tyr Glu Leu Gly Phe Ala Ser Gly Ala Pro Ser Asn Val 
    370                 375                 380                 
Arg Pro Val Thr Thr Thr Thr Gly Ala Gln Leu Val Ala Lys Ser Ile 
385                 390                 395                 400 
Tyr Gly Val Ala Ile Ala Gln Asn Gln Ser Ser Ala Gly Ile Ile Phe 
                405                 410                 415     
Met Ser Lys Gly Met Val Ser Thr Pro Gly Val Ala Ala Thr Thr Tyr 
            420                 425                 430         
Thr Pro Gln Pro Ser Ala Ile Val Thr Thr Pro Gly Thr Pro Val Ala 
        435                 440                 445             
Ala Pro Ile Gly Lys Asn Thr Pro Ile Met Phe Ser Ala Val Val Arg 
    450                 455                 460                 
Arg Thr Gly Asp Val Asn Ala Gly Pro Gly Ser Ala Asn Gly Thr Gln 
465                 470                 475                 480 
Tyr Gly Val Gly Ser Gln Pro Leu Ser Val Thr Leu Gly Leu Ser Leu 
                485                 490                 495     
Thr Asn Tyr Ser Ser Ala Leu Gln Pro Gly Gln Phe Phe Val Trp Gln 
            500                 505                 510         
Leu Asn Phe Ala Ser Gly Phe Met Glu Ile Gly Leu Asn Thr Asp Gly 
        515                 520                 525             
Tyr Phe Tyr Ala Gly Thr Gly Ala Tyr Ser Gly Met Ile Glu Leu Thr 
    530                 535                 540                 
Asp Leu Ile Asp Val Arg Pro Val Gly Val Arg Pro Asn Thr Ser Thr 
545                 550                 555                 560 
Leu Val Phe Asn Leu Thr Gly Val Ala Thr Thr Gly Tyr Ser Tyr Val 
                565                 570                 575     
Ala Gly His His His His His His 
            580                 

<210> 2
<211> 1755
<212> DNA
<213> Baculoviridae


<220> 
<223> E-VP60-His/pOET2C

<400> 2
atggagggta agcctcgggc tgacgcccct ggtaccgcta ccacagcatc tgttcctggg      60

accactaccg acggcatgga cccaggggtg gtggccagca ctgacgttgt gacggctgac     120

aacgttgcag catctgttgc cactgcgggg attggaggac caccacaaca ggtctcacct     180

caagagtctt ggcgtgcgaa cttcttttac aatgatgtat ttacctggtc agtcaccgat     240

gcacccggta gcattcttta cacagttcaa cactccccgc aaaacaaccc cttcacgcag     300

gtgctgagcc aaatgtatgc tggttgggct ggtggcatgc agtttagatt catagttgct     360

gggtctggta tttttggcgg aaggctcgtg tgtgcaatca taccacctgg gatccagata     420

caacctgggc ttgaggttag acagtttcct catgttgtca tcgatgcacg ctcacttgag     480

ccagtgacta taacaatgcc tgatcttcgt cctgaaatgt accacccaac aggcaatcct     540

ggacttgtcc caacactcgt cgtgagtgtg tacaacaact tgatcaaccc ctttgggggt     600

accaccagtg caattcaggt tactgttgag actaggccaa gtgaggactt tgagtttgtg     660

ttgatccgcg caccttcaag taaaacagtg gactcagtga acccctcgtg gctcttgaca     720

actccggtct tgaccggtgc tggttcagac aacagatggg gtgcaccaat tgtcgggctc     780

cagcccgtac caggtggctt ctccacttcc aacaggcact ggaacatgaa tggtgaaacc     840

tacggttggt ccagccccag gtttgacgac attgatcatc caagtggcaa tgtatcctac     900

cccactggta gtgcaaccaa caccattgag acctggtatg ccaatgctgg aacagctacc     960

accaacccaa tctccaacgt agcaccagac gggtttcctg acatgggagc catcccgttc    1020

agtggctcaa ccatcccgac tggtgcctgg gttgggtttg ggcaggtgtg gaacgcctcc    1080

aatggcaccc catacgtggg gaccgttcag gcgtacgagc tggggttcgc aagcggggcc    1140

ccgtccaacg ttcgtcctgt cacaacaacc acgggtgcgc agctggttgc aaagtcgatc    1200

tatggggttg ccattgccca aaaccaatcc agtgctggca ttatcttcat gtcgaaaggg    1260

atggtctcaa cacctggggt ggctgccaca acatacaccc cgcaaccaag cgcaattgtc    1320

acaacacctg gaacacctgt ggctgcaccc attggcaaga atacacctat tatgttcagt    1380

gcagtcgtgc ggaggactgg tgatgtgaac gccggtcctg ggtccgctaa cggcacacag    1440

tatggcgttg gctcgcagcc attgtcagta acacttggac tgtccctgac aaactactcc    1500

tccgcacttc aacctggcca gttctttgtg tggcaactca acttcgcttc tggcttcatg    1560

gagattggtt tgaatacaga tggttacttt tatgcaggga ctggtgctta ctcaggcatg    1620

attgaattga cagacctcat tgatgtgcgg cccgtgggag tccggccaaa cacgagcaca    1680

ctcgtgttca accttaccgg agtggccacc actggatatt cctatgtcgc cggtcatcat    1740

caccatcacc attga                                                     1755


<210> 3
<211> 40
<212> DNA
<213> Baculoviridae


<220> 
<223> EBHS-VP60-F

<400> 3
gatagtctcg aggccaccat ggagggtaag cctcgggctg                           40


<210> 4
<211> 32
<212> DNA
<213> Baculoviridae


<220> 
<223> EBHS-VP60/NgoMiv-R

<400> 4
cctaggccgg cgacatagga atatccagtg gt                                   32


<210> 5
<211> 21
<212> DNA
<213> Baculoviridae


<220> 
<223> EBHS-VP60/ApaLI-R

<400> 5
gagcccgaca attggtgcac c                                              21


<210> 6
<211> 21
<212> DNA
<213> Baculoviridae


<220> 
<223> EBHS-VP60/ApaLI-F

<400> 6
cagacaacag gtggggtgca c                                              21


