                               SEQUENCE LISTING

<110> KVAM, ERIK LEEMING
      NELSON, JOHN RICHARD
      GAO, WEI 
 
<120> CELL-FREE PROTEIN EXPRESSION USING DOUBLE-STRANDED CONCATAMERIC 
      DNA

<130> 316478-1

<140>
<141>

<160> 9     

<170> PatentIn version 3.5

<210> 1
<211> 21
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(3)
<223> May or may not be present

<400> 1
Gly Ser Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu 
1               5                   10                  15      


Glu Asn Pro Gly Pro 
            20      


<210> 2
<211> 22
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(3)
<223> May or may not be present

<400> 2
Gly Ser Gly Ala Thr Asn Phe Ser Leu Leu Lys Gln Ala Gly Asp Val 
1               5                   10                  15      


Glu Glu Asn Pro Gly Pro 
            20          


<210> 3
<211> 23
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(3)
<223> May or may not be present

<400> 3
Gly Ser Gly Gln Cys Thr Asn Tyr Ala Leu Leu Lys Leu Ala Gly Asp 
1               5                   10                  15      


Val Glu Ser Asn Pro Gly Pro 
            20              


<210> 4
<211> 25
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide


<220>
<221> MOD_RES
<222> (1)..(3)
<223> May or may not be present

<400> 4
Gly Ser Gly Val Lys Gln Thr Leu Asn Phe Asp Leu Leu Lys Leu Ala 
1               5                   10                  15      


Gly Asp Val Glu Ser Asn Pro Gly Pro 
            20                  25  


<210> 5
<211> 1395
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 5
ccgggatccc agtgaattgt aatacgactc actatagggc gaattaattc cggttatttt       60

ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga      120

cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg      180

tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt      240

gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat      300

aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg      360

aaagagtcaa atggctcacc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg      420

taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt      480

cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac      540

acgatgataa tatggccaca accgtgagca agggcgagga gctgttcacc ggggtggtgc      600

ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg      660

gtgaaggcga cgctacttac ggaaagctga cactgaagtt catttgcacc acaggcaaac      720

tgcctgtgcc ctggccaacc ctcgtgacta ccctcacata cggcgtgcag tgctttagca      780

gatatcctga tcatatgaaa cagcacgact tctttaagtc tgctatgcct gaaggatacg      840

tgcaggagag aaccatcttc ttcaaggacg acggaaacta taagactaga gccgaggtga      900

agtttgaggg agacacactg gtgaatagga tcgagctgaa gggcattgac ttcaaggagg      960

acggaaacat cctgggccac aagctggagt acaactacaa tagccacaac gtctatatta     1020

tggctgataa gcagaagaac ggaatcaagg tgaacttcaa gatcagacac aacatcgagg     1080

acggcagcgt gcagctggcc gaccactacc agcagaatac ccctatcgga gacggccccg     1140

tgctcctgcc agacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg     1200

agaagcgcga tcatatggtt ctgctggagt tcgtgaccgc cgccggcatc actcttggta     1260

tggacgagct gtacaagtaa taagatctga ctgaaaaaaa aaaagtttaa acactagtcc     1320

gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg     1380

ctgcagagat ctccg                                                      1395


<210> 6
<211> 6
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: 
      Synthetic oligonucleotide


<220>
<221> modified_base
<222> (1)..(2)
<223> A, C, G, or T/U 

<220>
<221> modified_base
<222> (1)..(2)
<223> Locked nucleic acid

<220>
<221> modified_base
<222> (3)..(5)
<223> 2-amino dA, 2-thio dT, normal G or normal C 

<220>
<221> misc_feature
<222> (5)..(6)
<223> Phosphorothioate linkage between nucleotides 

<220>
<221> modified_base
<222> (6)..(6)
<223> A, C, G, or T/U 

<400> 6
nnnnnn                                                                   6


<210> 7
<211> 6
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<220>
<223> Description of Combined DNA/RNA Molecule: 
      Synthetic oligonucleotide


<220>
<223> 5'-biotin

<220>
<221> modified_base
<222> (1)..(6)
<223> A, C, G, or T/U 

<220>
<221> misc_feature
<222> (4)..(6)
<223> Phosphorothioate linkage between nucleotides 

<400> 7
nnnnnn                                                                   6


<210> 8
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 8
His His His His His His 
1               5       


<210> 9
<211> 8
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      peptide

<400> 9
Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


