                         SEQUENCE LISTING

<110>  Autolus Limited
 
<120>  CELL

<130>  P112493PCT

<150>  GB 1712407.4
<151>  2017-08-02

<150>  GB 1806372.7
<151>  2018-04-19

<160>  32    

<170>  PatentIn version 3.5

<210>  1
<211>  1096
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD4 promoter

<400>  1
aagacaggtt ctcactctgt cactcaggct agagtgcagt ggtgcaatca cggttcactg       60

cagcctcaac ttcctgggct caagcgatcc ccccacctcg gcctcctaaa atgctgggat      120

tataggcatg agccaccact cccagcccca cttttttcag actggaaaac gcacactcac      180

atgtgcatct ttaaatgatc acttgggctg tggtatggag aatggcgacc agtgaggagg      240

caggagctgt tgtccgagca agggatgata ttggcatctt ggattggcat ggtggcagta      300

gtggtagtgc agagtgactt gggtagattt tggagccatt tagaaggtaa catccacagg      360

aactggtaaa taaatacgtg ggagaagttg ggtgaagggg gtgtcaaaga ttacacccaa      420

tttattttgc ttgggcaagt tggtggatgg tgagcccctc actgagtgag aagcctggag      480

aagcaggttt ggagggtggt agtatgcagg tggtatgcat agttggggat gtgtgttgag      540

tttgctatgt ccggtgagct tcccagtgga gatgtccaat gggcagacgg atactcacat      600

agagagttca tggtagattc gggctagagg aaagcacctg aggcctggcc agagacgcct      660

agaggaacag agcctggtta acagtcactc ctggtgtctc agatattctc tgctcagccc      720

acgccctctc ttccacactg ggccacctat aaagcctcca cagatacccc tggggcaccc      780

actggacaca tgccctcagg gccccagagc aaggagctgt ttgtgggctt accactgctg      840

ttcccatatg cccccaactg cctcccactt ctttccccac agcctggtca gacatggcgc      900

taccactaat ggaatctttc ttgccatctt tttcttgccg cttaacagtg gcagtgacag      960

tttgactcct gatttaagcc tgattctgct taactttttc ccttgacttt ggcattttca     1020

ctttgacatg ttccctgaga gcctgggggg tggggaaccc agctccagct ggtgacgttt     1080

ggggccggcc caggcc                                                     1096


<210>  2
<211>  540
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD8 promoter

<400>  2
cacaggaggc tcagcactaa tcggtagata ctgcgagatg ctgggaggtt aaggggccta       60

cccgcaatat ctctggccaa tgccttgggc tagaaatgcc ataattagcc gctcttttga      120

tcccttgcaa aatgcgaatc ccaccgcacc tccaccccac ccgagtggta atctcctagt      180

ggtaatctaa gtgagcctgt gataagataa gtagctcctg gtggtgaggg tgagaaattg      240

gggagctgga gccccagcca gggacgaggc tgtaggggct agggcgaaga tggaggctgc      300

tgggccccca gatggaagac ggtaacgtgc gcccgcttcg tttttgctcg aggtcagtca      360

ggtgcagact gaattcgaag tcgctccctc ctccgctcaa ccccgaccag gccaaaacta      420

aagcagcacc gccccctgct gggccgacag ggcatcagat tttgctggac gcgggtgaca      480

ggcgagatag ggagtgtccc tgctgctagt gcccctgctg ctagtgccta gttacctgca      540


<210>  3
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  FOXP3 promoter

<400>  3
tcccatccac acatagagct tcagattctc tttctttccc cagagaccct caaatatcct       60

ctcactcaca gaatggtgtc tctgcctgcc tcgggttggc cctgtgattt attttagttc      120

ttttcccttg tttttttttt ttcaaactct atacactttt gttttaaaaa ctgtggtttc      180

tcatgagccc tattatctca ttgatacctc tcacctctgt ggtgagggga agaaatcata      240

ttttcagatg actcgtaaag ggcaaagaaa aaaacccaaa atttcaaaat ttccgtttaa      300

gtctcataat caagaaaagg agaaacacag agagagagaa aaaaaaaact atgagaaccc      360

ccccccaccc cgtgattatc agcgcacaca ctcatcgaaa aaaatttgga ttattagaag      420

agagaggtct gcggcttcca caccgtacag cgtggttttt cttctcggta taaaagcaaa      480

gttgtttttg atacgtgaca gtttcccaca agccaggctg atccttttct gtcagtccac      540

ttcaccaagg tgagtgtccc tgctctcccc taccagatgt gggccccatt ggaggagatg      600


<210>  4
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD44 promoter

<400>  4
gaagttgtat gggaagatga atagaagaat aggtggttga ataaattaaa aggtgtgtgg       60

ttggatgaat gaatgagtgg gatgatagat ggacctaagt ggttagtgga tggacaggag      120

gatggatgga tgtgagagcc ccagaaggac ataaggaaag atgggtggat agatggatgg      180

gcggatggaa ggatatttag gaggatgaat gagcatgtgt gtggagagag gtgcccattc      240

acactggctt gaacacatgg gttagctgag ccaaatgcca gccctatgac aggccatcag      300

tagctttccc tgagctgttc tgccaagaag ctaaaattca ttcaagccat gtggacttgt      360

tattgagggg aaaaagaatg agctctccct ctttccactt ggaagattca ccaactcccc      420

acccctcact ccccactgtg ggcacggagg cactgcgcca cccagggcaa gacctcgccc      480

tctctccagc tcctctccca ggatatccaa catcctgtga aacccagaga tcttgctcca      540

gccggattca gagaaattta gcgggaaagg agaggccaaa ggctgaaccc aatggtgcaa      600


<210>  5
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD27 promoter region

<400>  5
ttttgtggtg ctggtttctg tataaacctg aaaaattctg aattccaaaa cttatctgac       60

ccccaaagtt tcagataaga gcttgtggac ctgtgctcaa ttctggttct ccttccttct      120

ttcaactgtt gtctgtgaaa ggagggatgc aggtatggga gacaggagtc ctgcgaattc      180

gtctgtaaac tgtggacggg ggggtgggtg ggggggggta acgtgggcac ctttgtgcac      240

aagtgcatga ataggagggg tgagcaactg tgtgtccatc acctttttgt caaagaagca      300

ggagtcagtg ggctacgtgc ttcatgagca ggagaggcgg aaactaagga aggctcatgt      360

gttggaggaa gcatgtttga agagcagcag gtctcacaga gtttgctctt taatactctc      420

cccagcacac ggaaggggaa gggggtggag gttgctgcta tgagagagaa aaaaaaaaca      480

gccacaatag agattctgcc ttcaaaggtt ggcttgccac ctgaagcagc cactgcccag      540

ggggtgcaaa gaagagacag cagcgcccag cttggaggtg ctaactccag aggccagcat      600


<210>  6
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD28 promoter region

<400>  6
caggtaccca ccatgatgcc tggctaattt tttgtatttt caatggagac ggggtttcac       60

catgttggcc aggctcgtct tgacctcctg gcctcaaatg atccacccac tttggcctcc      120

caaattgctg gcattacagg cgtgagccac tgcacccggc ctgttccttc ttaagaacac      180

tttgtctccc ctttaatctc tgctggattt caagcacccc ttttacacaa ctcttgatat      240

ccatcaataa agaataattc ccataagccc atcatgtagt gaccgactat ttttcagtga      300

caaaaaaaaa gtctttaaaa atagaagtaa aagtctaaag tcatcaaaac aacgttatat      360

cctgtgtgaa atgctgcagt caggatgcct tgtggtttga gtgccttgat catgtgccct      420

aaggggatgg tggcggtggt ggtggccgtg gatgacggag actctcaggc cttggcaggt      480

gcgtctttca gttcccctca cacttcgggt tcctcgggga ggaggggctg gaaccctagc      540

ccatcgtcag gacaaagatg ctcaggctgc tcttggctct caacttattc ccttcaattc      600


<210>  7
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD127 promoter region

<400>  7
cgagacaagc ctggccaaca tggcgaaacc ccgtctccac tgaaaacaca aaaattaggc       60

tggcatagtg gcatttgcct gtagtcctag ctactcagga ggctgaggca ggagaattgc      120

ttgaacctgg gaggtggaaa ttgcagtgag ccgagatcat gctattgtac tccagcctgg      180

gcaacaaagc aagactctgt ctcaaaaaaa taaaaattaa aaaaataaag tagcctctag      240

cctaagatag cttgagccta ggtgtgaatc tactgcctta ctctgatgta agcacagtaa      300

gtgtgggggc tgcagggaat atccaggagg aacaataatt tcagaggctc tgtctcttca      360

tgtccttgac ctctgcttac agcagcaata cttttactca gacttcctgt ttctggaact      420

tgccttcttt tttgctgtgt ttatacttcc cttgtctgtg gttagataag tataaagccc      480

tagatctaag cttctctgtc ttcctccctc cctcccttcc tcttactctc attcatttca      540

tacacactgg ctcacacatc tactctctct ctctatctct ctcagaatga caattctagg      600


<210>  8
<211>  600
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD122 promoter

<400>  8
tgctaaacgg agtaaggggc ttcctggaag gctgggtgaa atgggagtct cggaaagatg       60

gtgtgttgca ggctgggagg agggtgagac gctggggtca cctagaggga cctgcttgtg      120

tgaagcctac gtattagtgg gtatgtgtgt gaccggatgg aggcgtcaga ggtgttgggt      180

agcctgtgtg agttggcgtg ggggtgatgt aggaggggag agagggaggg cctgcgttcc      240

cttggctcct gtgtgcagct aggcccctat ttgacaatgt gtgtctgtgt gtgtgtgtgt      300

gtgtgtgtgt gtgtgtgtgt gtgtgtgtgc cgcccccagc gtaggaggca gatctttatc      360

tggccctggg tgcttgagga gtttcaggct ttctcataag cctcgtctcc ccgcctctcc      420

accccaggcc ttgcccctct atcctctgca caggaagtgg gctggctctg ggcttttagt      480

ctttgcggcc ccagcagcca gagctcagca gggccctgga gagatggcca cggtcccagc      540

accggggagg actggagagc gcgcgctgcc accgccccat gtctcagcca ggtgatgtcc      600


<210>  9
<211>  280
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70 complete SH2 domain

<400>  9

Met Pro Asp Pro Ala Ala His Leu Pro Phe Phe Tyr Gly Ser Ile Ser 
1               5                   10                  15      


Arg Ala Glu Ala Glu Glu His Leu Lys Leu Ala Gly Met Ala Asp Gly 
            20                  25                  30          


Leu Phe Leu Leu Arg Gln Cys Leu Arg Ser Leu Gly Gly Tyr Val Leu 
        35                  40                  45              


Ser Leu Val His Asp Val Arg Phe His His Phe Pro Ile Glu Arg Gln 
    50                  55                  60                  


Leu Asn Gly Thr Tyr Ala Ile Ala Gly Gly Lys Ala His Cys Gly Pro 
65                  70                  75                  80  


Ala Glu Leu Cys Glu Phe Tyr Ser Arg Asp Pro Asp Gly Leu Pro Cys 
                85                  90                  95      


Asn Leu Arg Lys Pro Cys Asn Arg Pro Ser Gly Leu Glu Pro Gln Pro 
            100                 105                 110         


Gly Val Phe Asp Cys Leu Arg Asp Ala Met Val Arg Asp Tyr Val Arg 
        115                 120                 125             


Gln Thr Trp Lys Leu Glu Gly Glu Ala Leu Glu Gln Ala Ile Ile Ser 
    130                 135                 140                 


Gln Ala Pro Gln Val Glu Lys Leu Ile Ala Thr Thr Ala His Glu Arg 
145                 150                 155                 160 


Met Pro Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys 
                165                 170                 175     


Leu Tyr Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg 
            180                 185                 190         


Lys Glu Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val 
        195                 200                 205             


Tyr His Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro 
    210                 215                 220                 


Glu Gly Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys 
225                 230                 235                 240 


Leu Lys Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys Pro Asn 
                245                 250                 255     


Ser Ser Ala Ser Asn Ala Ser Gly Ala Ala Ala Pro Thr Leu Pro Ala 
            260                 265                 270         


His Pro Ser Thr Leu Thr His Pro 
        275                 280 


<210>  10
<211>  93
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70 SH2 1

<400>  10

Phe Phe Tyr Gly Ser Ile Ser Arg Ala Glu Ala Glu Glu His Leu Lys 
1               5                   10                  15      


Leu Ala Gly Met Ala Asp Gly Leu Phe Leu Leu Arg Gln Cys Leu Arg 
            20                  25                  30          


Ser Leu Gly Gly Tyr Val Leu Ser Leu Val His Asp Val Arg Phe His 
        35                  40                  45              


His Phe Pro Ile Glu Arg Gln Leu Asn Gly Thr Tyr Ala Ile Ala Gly 
    50                  55                  60                  


Gly Lys Ala His Cys Gly Pro Ala Glu Leu Cys Glu Phe Tyr Ser Arg 
65                  70                  75                  80  


Asp Pro Asp Gly Leu Pro Cys Asn Leu Arg Lys Pro Cys 
                85                  90              


<210>  11
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ZAP70 SH2 2

<400>  11

Trp Tyr His Ser Ser Leu Thr Arg Glu Glu Ala Glu Arg Lys Leu Tyr 
1               5                   10                  15      


Ser Gly Ala Gln Thr Asp Gly Lys Phe Leu Leu Arg Pro Arg Lys Glu 
            20                  25                  30          


Gln Gly Thr Tyr Ala Leu Ser Leu Ile Tyr Gly Lys Thr Val Tyr His 
        35                  40                  45              


Tyr Leu Ile Ser Gln Asp Lys Ala Gly Lys Tyr Cys Ile Pro Glu Gly 
    50                  55                  60                  


Thr Lys Phe Asp Thr Leu Trp Gln Leu Val Glu Tyr Leu Lys Leu Lys 
65                  70                  75                  80  


Ala Asp Gly Leu Ile Tyr Cys Leu Lys Glu Ala Cys 
                85                  90          


<210>  12
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  xenobiotic recognition elements (XRE(s)) core sequence

<400>  12
gcgtg                                                                    5


<210>  13
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XRE consensus sequence


<220>
<221>  misc_feature
<222>  (2)..(2)
<223>  n is a, c, g, or t

<400>  13
kngcgtgmsa                                                              10


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  microRNA target sequence, miR-17

<400>  14
cctattccag cactttcaag tagctgtgat                                        30


<210>  15
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  microRNA target sequence, miR-146

<400>  15
agttcaacaa aagttctcac atggagtccc                                        30


<210>  16
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  microRNA target sequence, miR-214

<400>  16
aacttaccaa ggacaggcag gaccccgtcc                                        30


<210>  17
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  microRNA target sequence, miR-21

<400>  17
tttattactt tattggtgtt aaggataaca                                        30


<210>  18
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  microRNA target sequence, miR-181

<400>  18
tgctatgtag atttctgaat gtgttgtatt                                        30


<210>  19
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  microRNA target sequence, miR-9

<400>  19
ccctaccccc caacccctag cccaaccaat                                        30


<210>  20
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  microRNA target sequence, miR-29

<400>  20
cctttcacat tggtgctttt ccatttatgc                                        30


<210>  21
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  microRNA target sequence, miR-126

<400>  21
aaagaggttt ttaataatga ggtccttctg                                        30


<210>  22
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  microRNA target sequence, miR-326

<400>  22
gtctgctatt cccagagagg tctcagaggg                                        30


<210>  23
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  microRNA target sequence, miR-155

<400>  23
ctgcacttat tgtaggaaat tttaatatat                                        30


<210>  24
<211>  5
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XRE core sequence

<400>  24
cacgc                                                                    5


<210>  25
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XRE promoter

<400>  25
ctggtaagca cgccaatgaa                                                   20


<210>  26
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XRE promoter

<400>  26
tgagttctca cgctagcaga ttgagttctc acgctagcag attgagttct cacgctagca       60

gat                                                                     63


<210>  27
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  AP-1 responsive promoter

<400>  27
tgagtcagtg actcagtgag tcagtgactc agtgagtcag tgactcag                    48


<210>  28
<211>  87
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CREB responsive promoter

<400>  28
gcaccagaca gtgacgtcag ctgccagatc ccatggccgt catactgtga cgtctttcag       60

acaccccatt gacgtcaatg ggagaac                                           87


<210>  29
<211>  115
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SRE responsive promoter

<400>  29
aggatgtcca tattaggaca tctaggatgt ccatattagg acatctagga tgtccatatt       60

aggacatcta ggatgtccat attaggacat ctaggatgtc catattagga catct           115


<210>  30
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TCF-LEF responsive promoter

<400>  30
agatcaaagg gtttaagatc aaagggctta agatcaaagg gtataagatc aaagggccta       60

agatcaaagg gactaagatc aaagggttta agatcaaagg gcttaagatc aaagggccta      120


<210>  31
<211>  125
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  STAT3 responsive promoter

<400>  31
agcttcattt cccgtaaatc gtcgaagctt catttcccgt aaatcgtcga agcttcattt       60

cccgtaaatc gtcgaagctt catttcccgt aaatcgtcga agcttcattt cccgtaaatc      120

gtcga                                                                  125


<210>  32
<211>  75
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  STAT5 responsive promoter

<400>  32
agttctgaga aaagtagttc tgagaaaagt agttctgaga aaagtagttc tgagaaaagt       60

agttctgaga aaagt                                                        75


