               SEQUENCE LISTING

<110> OligoScience Biotechnology GmbH

<120> Microorganism for producing human milk oligosaccharide

<130> 198 377

<160> 42

<170> BiSSAP 1.3.6

<210> 1
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> FWD primer Pmgt

<400> 1
gtatactccg gagcaaacgc ctgaactccc                                     30


<210> 2
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> REV primer Pmgt

<400> 2
tctagaggaa gaataagtac gtgctatatt tag                                  33


<210> 3
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> FWD primer PmgtA

<400> 3
cgagctcctt tcgttattca gcacccg                                        27


<210> 4
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> REV primer PmgtA

<400> 4
cgagctcgcg atataatacc tgctggc                                        27


<210> 5
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> FWD primer mgtA 5'UTR

<400> 5
cgagctcctt tcgttattca gcacccg                                        27


<210> 6
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> REV primer mgtA 5'UTR

<400> 6
cgagctcaag gagtccctcc gcactgt                                        27


<210> 7
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> FWD primer SRRZ gene

<400> 7
ccgcatatgc cagaaaaaca tgacctg                                        27


<210> 8
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> REV primer SRRZ gene

<400> 8
ttagtcgacg caactctatc tgcactgctc                                     30


<210> 9
<211> 38
<212> DNA
<213> Artificial Sequence


<220> 
<223> SRRz-SacI-fw

<400> 9
cgagctcaag gagatataat gccagaaaaa catgacct                             38


<210> 10
<211> 50
<212> DNA
<213> Artificial Sequence


<220> 
<223> SRRz-luxTerm-PstI

<400> 10
cggctgcaga atctggcttt ttatattctc taagcgcgtg tgtattgctc                50


<210> 11
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> SacI-PmgtA-UTR-fw

<400> 11
ctcgagctcc tttcgttatt cagcacccg                                      29


<210> 12
<211> 27
<212> DNA
<213> Artificial Sequence


<220> 
<223> SacI-PmgtA-UTR-rv

<400> 12
cgagctcaag gagtccctcc gcactgt                                        27


<210> 13
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> Bsu36i-PmgtA-UTR-fw

<400> 13
cgcctcaggc tttcgttatt cagcacccg                                      29


<210> 14
<211> 53
<212> DNA
<213> Artificial Sequence


<220> 
<223> SRRz-luxTerm-Bsu36i

<400> 14
cagcctgagg aaataataaa aaagccggat taataatctg gctttttata ttc            53


<210> 15
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> zwf-BglII-fw

<400> 15
gagagatcta tggcggtaac gcaaacagc                                      29


<210> 16
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> zwf-KpnI-rv

<400> 16
gcgggtacct tactcaaact cattccagga ac                                   32


<210> 17
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> gsk-KpnI-fw

<400> 17
gtaaaaggta ccatgaaatt tcccggtaaa cgt                                  33


<210> 18
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> gsk_XhoI-rv

<400> 18
cgacctcgag ttaacgatcc cagtaagact c                                   31


<210> 19
<211> 26
<212> DNA
<213> Artificial Sequence


<220> 
<223> rcsA-NdeI-fw

<400> 19
gtatcatatg tcaacgatta ttatgg                                         26


<210> 20
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> rcsA-BglII-rv

<400> 20
ccaagatcta tgtgttagcg catgttgac                                      29


<210> 21
<211> 34
<212> DNA
<213> Artificial Sequence


<220> 
<223> lacY-BamHI-fw

<400> 21
gaaaggatcc atgtactatt taaaaaacac aaac                                 34


<210> 22
<211> 28
<212> DNA
<213> Artificial Sequence


<220> 
<223> lacY-HindIII-rv

<400> 22
cattaagctt ttaagcgact tcattcac                                       28


<210> 23
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> lacY-S209I-fw

<400> 23
attcggcatt tattcttaag ctggcactgg aactg                                35


<210> 24
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> lacY-S209I-rv

<400> 24
cttaagaata aatgccgaat ggttggcacc                                     30


<210> 25
<211> 35
<212> DNA
<213> Artificial Sequence


<220> 
<223> trxA-BglII-fw

<400> 25
gagagatcta tgagcgataa aattattcac ctgac                                35


<210> 26
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> trxA-KpnI-rv

<400> 26
cgaaggtacc attcccttac gccaggttag                                     30


<210> 27
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> manCB-NcoI-fw

<400> 27
cataccatgg cgcagtcgaa actctatcca g                                   31


<210> 28
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> manCB-HindIII-rv

<400> 28
cccaagcttt ggggtaaggg aagatccgac                                     30


<210> 29
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> gmd-NdeI-fw

<400> 29
cgccatatgt caaaagtcgc tctcatcacc                                     30


<210> 30
<211> 31
<212> DNA
<213> Artificial Sequence


<220> 
<223> gmd-KpnI-rv

<400> 30
ggcggtacct tcctgacgta aaaacatcat t                                   31


<210> 31
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> pntAB-NcoI-fw

<400> 31
gggccatgga tgcgaattgg cataccaag                                      29


<210> 32
<211> 29
<212> DNA
<213> Artificial Sequence


<220> 
<223> pntAB-BamHI-rv

<400> 32
ccgggatcct tacagagctt tcaggattg                                      29


<210> 33
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> lacIZYA-koF13

<400> 33
gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta gtgtaggctg     60

gagctgcttc                                                            70


<210> 34
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> lacIZYA-koR13

<400> 34
ttaaactgac gattcaactt tataatcttt gaaataatag tgcttatccc gatccgtcga     60

cctgcagttc                                                            70


<210> 35
<211> 71
<212> DNA
<213> Artificial Sequence


<220> 
<223> wcaJ-koF13

<400> 35
atgacaaatc taaaaaagcg cgagcgagcg aaaaccaatg catcgttaat cgtgtaggct     60

ggagctgctt c                                                          71


<210> 36
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> wcaJ-koR13

<400> 36
tcaatatgcc gctttgttaa cgaagccctt gaataccgtc aggaaaacga gatccgtcga     60

cctgcagttc                                                            70


<210> 37
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> lon-koF13

<400> 37
cagtcgtgtc atctgattac ctggcggaaa ttaaactaag agagagctct gtgtaggctg     60

gagctgcttc                                                            70


<210> 38
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> lon-koR13

<400> 38
cgaattagcc tgccagccct gtttttatta gtgcattttg cgcgaggtca gatccgtcga     60

cctgcagttc                                                            70


<210> 39
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> clpYQ-koF13

<400> 39
tacttttgta cggggtttgt actctgtatt cgtaaccaag gggtcagctc gtgtaggctg     60

gagctgcttc                                                            70


<210> 40
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> clpYQ-koR13

<400> 40
gcctttcagc cccatcaaac aatgatgaaa atgattgaac gcgattatag gatccgtcga     60

cctgcagttc                                                            70


<210> 41
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> trxB-koF13

<400> 41
atctcatggg cacgaccaaa cacagtaaac tgcttatcct gggttcaggc gtgtaggctg     60

gagctgcttc                                                            70


<210> 42
<211> 70
<212> DNA
<213> Artificial Sequence


<220> 
<223> trxB-koR13

<400> 42
gcatggtgtc gccttcttta cttttgttac tgatttgtaa aattattttg gatccgtcga     60

cctgcagttc                                                            70


