                         SEQUENCE LISTING

<110>  The Hospital for Sick Children
 
<120>  NANOPARTICLE PLATFORM FOR ANTIBODY AND VACCINE DELIVERY

<130>  3206-5012 (145935) ELL

<140>  PCT/CA2018/050954
<141>  2018-08-03

<150>  US 62/541209
<151>  2017-08-04

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antigen

<400>  1

Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
1               5                   10                  15      


Asn Pro Asn Ala Asn Pro 
            20          


<210>  2
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  2

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Asp Gly Ser Lys Lys Tyr Tyr Ala Asp Ser Val Lys 
    50                  55                  60                  


Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys Asn Thr Leu Tyr Leu 
65                  70                  75                  80  


Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala 
                85                  90                  95      


Arg Val Arg Asp Ser Ser Asp Tyr Tyr Gly Asp Ala Phe Asp Ile Trp 
            100                 105                 110         


Gly Gln Gly Thr Met Val Thr Val Ser Ser 
        115                 120         


<210>  3
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Epitope

<400>  3

Lys Gln Pro Ala Asp Gly Asn Pro Asp Pro Asn Ala Asn Pro Asn Val 
1               5                   10                  15      


Asp Pro Asn 
            


<210>  4
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Flag Tag

<400>  4

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  5
<211>  710
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  5

Tyr Glu Leu Thr Gln Glu Thr Gly Val Ser Val Ala Leu Gly Arg Thr 
1               5                   10                  15      


Val Thr Ile Thr Cys Arg Gly Asp Ser Leu Arg Ser His Tyr Ala Ser 
            20                  25                  30          


Trp Tyr Gln Lys Lys Pro Gly Gln Ala Pro Ile Leu Leu Phe Tyr Gly 
        35                  40                  45              


Lys Asn Asn Arg Pro Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Ala 
    50                  55                  60                  


Ser Gly Asn Arg Ala Ser Leu Thr Ile Ser Gly Ala Gln Ala Glu Asp 
65                  70                  75                  80  


Asp Ala Glu Tyr Tyr Cys Ser Ser Arg Asp Lys Ser Gly Ser Arg Leu 
                85                  90                  95      


Ser Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Ser Gln Pro Lys 
            100                 105                 110         


Ala Ala Pro Ser Val Thr Leu Phe Pro Pro Ser Ser Glu Glu Leu Gln 
        115                 120                 125             


Ala Asn Lys Ala Thr Leu Val Cys Leu Ile Ser Asp Phe Tyr Pro Gly 
    130                 135                 140                 


Ala Val Thr Val Ala Trp Lys Ala Asp Ser Ser Pro Val Lys Ala Gly 
145                 150                 155                 160 


Val Glu Thr Thr Thr Pro Ser Lys Gln Ser Asn Asn Lys Tyr Ala Ala 
                165                 170                 175     


Ser Ser Tyr Leu Ser Leu Thr Pro Glu Gln Trp Lys Ser His Arg Ser 
            180                 185                 190         


Tyr Ser Cys Gln Val Thr His Glu Gly Ser Thr Val Glu Lys Thr Val 
        195                 200                 205             


Ala Pro Thr Glu Cys Gly Gly Ser Ser Gly Ser Gly Ser Gly Ser Thr 
    210                 215                 220                 


Gly Glu Asn Leu Tyr Phe Gln Gly Ser Ala Gly Thr Thr Gly Thr Ser 
225                 230                 235                 240 


Ala Ser Thr Ser Gly Tyr Pro Tyr Asp Val Pro Asp Tyr Ala Gly Gly 
                245                 250                 255     


Gly Gly Ser Ala Gly Gly Thr Ala Thr Leu Glu Val Leu Phe Gln Gly 
            260                 265                 270         


Pro Ser Ser Gly Ser Ser Ser Ser Gly Gly Thr Gly Glu Val Gln Leu 
        275                 280                 285             


Val Glu Ser Gly Gly Gly Leu Val Lys Pro Gly Gly Ser Leu Arg Leu 
    290                 295                 300                 


Ser Cys Ser Ala Ser Gly Phe Asp Phe Asp Asn Ala Trp Met Thr Trp 
305                 310                 315                 320 


Val Arg Gln Pro Pro Gly Lys Gly Leu Glu Trp Val Gly Arg Ile Thr 
                325                 330                 335     


Gly Pro Gly Glu Gly Trp Ser Val Asp Tyr Ala Ala Pro Val Glu Gly 
            340                 345                 350         


Arg Phe Thr Ile Ser Arg Leu Asn Ser Ile Asn Phe Leu Tyr Leu Glu 
        355                 360                 365             


Met Asn Asn Leu Arg Met Glu Asp Ser Gly Leu Tyr Phe Cys Ala Arg 
    370                 375                 380                 


Thr Gly Lys Tyr Tyr Asp Phe Trp Ser Gly Tyr Pro Pro Gly Glu Glu 
385                 390                 395                 400 


Tyr Phe Gln Asp Trp Gly Arg Gly Thr Leu Val Thr Val Ser Ser Ala 
                405                 410                 415     


Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys Ser 
            420                 425                 430         


Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr Phe 
        435                 440                 445             


Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser Gly 
    450                 455                 460                 


Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser Leu 
465                 470                 475                 480 


Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr Tyr 
                485                 490                 495     


Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys Arg 
            500                 505                 510         


Val Glu Pro Lys Ser Cys Ser Arg Gly Gly Gly Gly Gly Ser Gly Gly 
        515                 520                 525             


Ser Gly Gly Ser Gly Gly Ser Met Ser Ser Gln Ile Arg Gln Asn Tyr 
    530                 535                 540                 


Ser Thr Asp Val Glu Ala Ala Val Asn Ser Leu Val Asn Leu Tyr Leu 
545                 550                 555                 560 


Gln Ala Ser Tyr Thr Tyr Leu Ser Leu Gly Phe Tyr Phe Asp Arg Asp 
                565                 570                 575     


Asp Val Ala Leu Glu Gly Val Ser His Phe Phe Arg Glu Leu Ala Glu 
            580                 585                 590         


Glu Lys Arg Glu Gly Tyr Glu Arg Leu Leu Lys Met Gln Asn Gln Arg 
        595                 600                 605             


Gly Gly Arg Ala Leu Phe Gln Asp Ile Lys Lys Pro Ala Glu Asp Glu 
    610                 615                 620                 


Trp Gly Lys Thr Pro Asp Ala Met Lys Ala Ala Met Ala Leu Glu Lys 
625                 630                 635                 640 


Lys Leu Asn Gln Ala Leu Leu Asp Leu His Ala Leu Gly Ser Ala Arg 
                645                 650                 655     


Thr Asp Pro His Leu Cys Asp Phe Leu Glu Thr His Phe Leu Asp Glu 
            660                 665                 670         


Glu Val Lys Leu Ile Lys Lys Met Gly Asp His Leu Thr Asn Leu His 
        675                 680                 685             


Arg Leu Gly Gly Pro Glu Ala Gly Leu Gly Glu Tyr Leu Phe Glu Arg 
    690                 695                 700                 


Leu Thr Leu Arg His Asp 
705                 710 


<210>  6
<211>  718
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Nanoparticle

<400>  6

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Lys Gly Gly Ser Ser Gly Ser Gly Ser Gly Ser Thr Gly Thr 
225                 230                 235                 240 


Ser Ser Ser Gly Thr Gly Thr Ser Ala Gly Thr Thr Gly Thr Ser Ala 
                245                 250                 255     


Ser Thr Ser Gly Ser Gly Ser Gly Gly Gly Gly Gly Ser Gly Gly Gly 
            260                 265                 270         


Gly Ser Ala Gly Gly Thr Ala Thr Ala Gly Ala Ser Ser Gly Ser Gly 
        275                 280                 285             


Ser Ser Gly Ser Ser Ser Ser Gly Gly Thr Gly Asp Lys Thr His Thr 
    290                 295                 300                 


Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe 
305                 310                 315                 320 


Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro 
                325                 330                 335     


Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu Val 
            340                 345                 350         


Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr 
        355                 360                 365             


Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser Val 
    370                 375                 380                 


Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys 
385                 390                 395                 400 


Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser 
                405                 410                 415     


Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro 
            420                 425                 430         


Ser Arg Glu Glu Met Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val 
        435                 440                 445             


Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly 
    450                 455                 460                 


Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp 
465                 470                 475                 480 


Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp 
                485                 490                 495     


Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu His 
            500                 505                 510         


Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys Ser Arg 
        515                 520                 525             


Gly Gly Gly Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Met 
    530                 535                 540                 


Ser Ser Gln Ile Arg Gln Asn Tyr Ser Thr Asp Val Glu Ala Ala Val 
545                 550                 555                 560 


Asn Ser Leu Val Asn Leu Tyr Leu Gln Ala Ser Tyr Thr Tyr Leu Ser 
                565                 570                 575     


Leu Gly Phe Tyr Phe Asp Arg Asp Asp Val Ala Leu Glu Gly Val Ser 
            580                 585                 590         


His Phe Phe Arg Glu Leu Ala Glu Glu Lys Arg Glu Gly Tyr Glu Arg 
        595                 600                 605             


Leu Leu Lys Met Gln Asn Gln Arg Gly Gly Arg Ala Leu Phe Gln Asp 
    610                 615                 620                 


Ile Lys Lys Pro Ala Glu Asp Glu Trp Gly Lys Thr Pro Asp Ala Met 
625                 630                 635                 640 


Lys Ala Ala Met Ala Leu Glu Lys Lys Leu Asn Gln Ala Leu Leu Asp 
                645                 650                 655     


Leu His Ala Leu Gly Ser Ala Arg Thr Asp Pro His Leu Cys Asp Phe 
            660                 665                 670         


Leu Glu Thr His Phe Leu Asp Glu Glu Val Lys Leu Ile Lys Lys Met 
        675                 680                 685             


Gly Asp His Leu Thr Asn Leu His Arg Leu Gly Gly Pro Glu Ala Gly 
    690                 695                 700                 


Leu Gly Glu Tyr Leu Phe Glu Arg Leu Thr Leu Arg His Asp 
705                 710                 715             


<210>  7
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  7

Ala Arg Val Gln Asp Ser Glu Asp Tyr Gly Gly Asn Ser Gly Ala Phe 
1               5                   10                  15      


Asp Ile 
        


<210>  8
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  8

Ala Arg Val Arg Asp Ser Ser Asp Tyr Tyr Gly Asp Ala Phe Asp Ile 
1               5                   10                  15      


<210>  9
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  9

Ala Arg Val Gln Thr Thr Thr Gly Gly Gly Ser Cys Cys Pro Phe Asp 
1               5                   10                  15      


Tyr 
    


<210>  10
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  10

Ala Arg Val Gln Asp Ser Glu Asp Tyr Gly Gly Asn Ser Gly Val Phe 
1               5                   10                  15      


Asp Ile 
        


<210>  11
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  11

Ala Lys Val Gly Glu Gly Gln Val Gly Asp Ser Ser Gly Tyr Tyr Asp 
1               5                   10                  15      


His 
    

<210>  12
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  12

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Trp Tyr Asp Gly Ser Asn Lys Tyr Tyr 
    50                  55                  60  


<210>  13
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  13

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr 
    50                  55                  60  


<210>  14
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  14

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr 
    50                  55                  60  


<210>  15
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  15

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Phe Ile Arg Tyr Asp Gly Ser Asn Lys Tyr Tyr 
    50                  55                  60  


<210>  16
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  16

Gln Val Gln Leu Val Asp Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Ala Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Ala Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Val Ile Ser Tyr Asp Gly Ser Asn Lys Tyr Tyr 
    50                  55                  60  


<210>  17
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  17

Gln Val Gln Leu Val Glu Ser Gly Gly Gly Val Val Gln Pro Gly Arg 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Ser Tyr 
            20                  25                  30          


Gly Met His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Phe Ile Arg Tyr Asp Gly Ser Asn Lys Tyr Tyr 
    50                  55                  60  


<210>  18
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  18

Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
1               5                   10                  15      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            20                  25                  30          


Val Tyr Tyr Cys Ala Arg 
        35              


<210>  19
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  19

Ala Asp Ser Ala Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Thr 
1               5                   10                  15      


Asn Thr Leu Phe Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            20                  25                  30          


Val Tyr Tyr Cys Ala Arg 
        35              


<210>  20
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  20

Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
1               5                   10                  15      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            20                  25                  30          


Val Tyr Tyr Cys Ala Lys 
        35              


<210>  21
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  21

Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
1               5                   10                  15      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Gly Thr Ala 
            20                  25                  30          


Val Tyr Tyr Cys Ala Arg 
        35              


<210>  22
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  22

Ala Asp Ser Val Lys Gly Arg Phe Ala Ile Ser Arg Asp Asn Ser Lys 
1               5                   10                  15      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            20                  25                  30          


Val Tyr Tyr Cys Ala Arg 
        35              


<210>  23
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  23

Thr Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
1               5                   10                  15      


Asn Thr Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            20                  25                  30          


Val Tyr Tyr Cys Ala Arg 
        35              


<210>  24
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  24

Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
1               5                   10                  15      


Asn Arg Leu Tyr Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala 
            20                  25                  30          


Val Tyr Tyr Cys Ala Arg 
        35              


<210>  25
<211>  38
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Antibody

<400>  25

Ala Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ser Lys 
1               5                   10                  15      


Asn Thr Leu Tyr Leu Gln Met Ser Ser Leu Arg Ala Glu Asp Thr Ala 
            20                  25                  30          


Val Tyr Tyr Cys Ala Arg 
        35              


