
                                SEQUENCE LISTING

<110> The Trustees of Princeton University
      Hecht, Michael
      Zarzhitsky, Shlomo

<120> Fusion Tags For Recombinant Protein
  Expression

<130> 5391.1006001

<150> 62/538,505          
<151> 2017-07-28  

<160> 14

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 102
<212> PRT
<213> Artificial Sequence

<220> 
<223> S-824 DEEP fusion tag

<400> 1
Met Tyr Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu
 1               5                  10                  15      
Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His Asp
            20                  25                  30          
Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met
        35                  40                  45              
Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu Phe
    50                  55                  60                  
Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly Lys His
65                  70                  75                  80  
Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His Leu
                85                  90                  95      
Glu Glu Leu Val His Arg
            100         


<210> 2
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> FLAG octapeptide

<400> 2
Asp Tyr Lys Asp Asp Asp Asp Lys
 1               5              


<210> 3
<211> 249
<212> PRT
<213> Artificial Sequence

<220> 
<223> DEEP-GFP

<400> 3
Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe Ser Lys Gly Glu
 1               5                  10                  15      
Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp
            20                  25                  30          
Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala
        35                  40                  45              
Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu
    50                  55                  60                  
Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln
65                  70                  75                  80  
Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg His Asp Phe Phe Lys
                85                  90                  95      
Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Ser Phe Lys
            100                 105                 110         
Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp
        115                 120                 125             
Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp
    130                 135                 140                 
Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn
145                 150                 155                 160 
Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe
                165                 170                 175     
Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His
            180                 185                 190         
Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp
        195                 200                 205             
Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu
    210                 215                 220                 
Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile
225                 230                 235                 240 
Thr His Gly Met Asp Glu Leu Tyr Lys
                245                 


<210> 4
<211> 29
<212> PRT
<213> Artificial Sequence

<220> 
<223> DEEP-TRP

<400> 4
Gly Gly Gly Gly Gly Gly Ser Arg Met Asn Leu Tyr Ile Gln Trp Leu
 1               5                  10                  15      
Lys Asp Gly Gly Pro Ser Ser Gly Arg Pro Pro Pro Ser
            20                  25                  


<210> 5
<211> 54
<212> PRT
<213> Artificial Sequence

<220> 
<223> DEEP-amyloid beta.sub.1-42

<400> 5
Gly Gly Gly Gly Gly Gly Ser Arg Ile Glu Gly Arg Asp Ala Glu Phe
 1               5                  10                  15      
Arg His Asp Ser Gly Tyr Glu Val His His Gln Lys Leu Val Phe Phe
            20                  25                  30          
Ala Glu Asp Val Gly Ser Asn Lys Gly Ala Ile Ile Gly Leu Met Val
        35                  40                  45              
Gly Gly Val Val Ile Ala
    50                  


<210> 6
<211> 30
<212> PRT
<213> Artificial Sequence

<220> 
<223> DEEP-LS3

<400> 6
Gly Gly Gly Gly Gly Gly Ser Arg Met Leu Ser Leu Leu Leu Ser Leu
 1               5                  10                  15      
Leu Ser Leu Leu Leu Ser Leu Leu Ser Leu Leu Leu Ser Leu
            20                  25                  30  


<210> 7
<211> 158
<212> PRT
<213> Artificial Sequence

<220> 
<223> DEEP-INSULIN

<400> 7
Met Lys Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala
 1               5                  10                  15      
Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr
            20                  25                  30          
Arg Arg Tyr Gly Lys Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val
        35                  40                  45              
Leu Lys Asn Leu His Lys Asn Trp His Gly Gly Lys Asp Asn Leu His
    50                  55                  60                  
Asp Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe
65                  70                  75                  80  
Met Gln Gly Gly Gly Ser Gly Gly Lys Leu Gln Glu Met Met Lys Glu
                85                  90                  95      
Phe Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly Lys
            100                 105                 110         
His Thr Val His His Ile Glu Gln Asn Ile Lys Glu Ile Phe His His
        115                 120                 125             
Leu Glu Glu Leu Val His Arg Lys Arg Gly Ile Val Glu Gln Cys Cys
    130                 135                 140                 
Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn
145                 150                 155             


<210> 8
<211> 30
<212> PRT
<213> Homo sapiens

<400> 8
Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala Leu Tyr
 1               5                  10                  15      
Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr
            20                  25                  30  


<210> 9
<211> 21
<212> PRT
<213> Homo sapiens

<400> 9
Gly Ile Val Glu Gln Cys Cys Thr Ser Ile Cys Ser Leu Tyr Gln Leu
 1               5                  10                  15      
Glu Asn Tyr Cys Asn
            20      


<210> 10
<211> 101
<212> PRT
<213> Artificial Sequence

<220> 
<223> S-824 DEEPhislys

<400> 10
Tyr Gly His Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val Leu His
 1               5                  10                  15      
Asn Leu His His Asn Trp His Gly Gly His Asp Asn Leu His Asp Val
            20                  25                  30          
Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe Met Gln
        35                  40                  45              
Gly Gly Gly Ser Gly Gly His Leu Gln Glu Met Met His Glu Phe Gln
    50                  55                  60                  
Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly His His Thr
65                  70                  75                  80  
Val His His Ile Glu Gln Asn Ile His Glu Ile Phe His His Leu Glu
                85                  90                  95      
Glu Leu Val His Arg
            100     


<210> 11
<211> 110
<212> PRT
<213> Homo sapiens

<400> 11
Met Ala Leu Trp Met Arg Leu Leu Pro Leu Leu Ala Leu Leu Ala Leu
 1               5                  10                  15      
Trp Gly Pro Asp Pro Ala Ala Ala Phe Val Asn Gln His Leu Cys Gly
            20                  25                  30          
Ser His Leu Val Glu Ala Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe
        35                  40                  45              
Phe Tyr Thr Pro Lys Thr Arg Arg Glu Ala Glu Asp Leu Gln Val Gly
    50                  55                  60                  
Gln Val Glu Leu Gly Gly Gly Pro Gly Ala Gly Ser Leu Gln Pro Leu
65                  70                  75                  80  
Ala Leu Glu Gly Ser Leu Gln Lys Arg Gly Ile Val Glu Gln Cys Cys
                85                  90                  95      
Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn
            100                 105                 110 


<210> 12
<211> 10
<212> PRT
<213> Artificial Sequence

<220> 
<223> linker

<400> 12
Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly
 1               5                  10  


<210> 13
<211> 8
<212> PRT
<213> Artificial Sequence

<220> 
<223> 6x glycine linker

<400> 13
Gly Gly Gly Gly Gly Gly Ser Arg
 1               5              


<210> 14
<211> 158
<212> PRT
<213> Artificial Sequence

<220> 
<223> insulin A-DEEPhislys-insulin B

<400> 14
Met Arg Phe Val Asn Gln His Leu Cys Gly Ser His Leu Val Glu Ala
 1               5                  10                  15      
Leu Tyr Leu Val Cys Gly Glu Arg Gly Phe Phe Tyr Thr Pro Lys Thr
            20                  25                  30          
Arg Arg Tyr Gly His Leu Asn Asp Leu Leu Glu Asp Leu Gln Glu Val
        35                  40                  45              
Leu His Asn Leu His His Asn Trp His Gly Gly His Asp Asn Leu His
    50                  55                  60                  
Asp Val Asp Asn His Leu Gln Asn Val Ile Glu Asp Ile His Asp Phe
65                  70                  75                  80  
Met Gln Gly Gly Gly Ser Gly Gly His Leu Gln Glu Met Met His Glu
                85                  90                  95      
Phe Gln Gln Val Leu Asp Glu Leu Asn Asn His Leu Gln Gly Gly His
            100                 105                 110         
His Thr Val His His Ile Glu Gln Asn Ile His Glu Ile Phe His His
        115                 120                 125             
Leu Glu Glu Leu Val His Arg Lys Arg Gly Ile Val Glu Gln Cys Cys
    130                 135                 140                 
Thr Ser Ile Cys Ser Leu Tyr Gln Leu Glu Asn Tyr Cys Asn
145                 150                 155             

