                         SEQUENCE LISTING

<110>  Oxford University Innovation Limited
 
<120>  MALARIA VACCINE

<130>  P10450WO

<150>  1712092.4
<151>  2017-07-27

<150>  1718337.7
<151>  2017-11-06

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  410
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  R21

<400>  1

Met Asp Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
1               5                   10                  15      


Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
            20                  25                  30          


Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
        35                  40                  45              


Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
    50                  55                  60                  


Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Lys Asn Asn Gln 
65                  70                  75                  80  


Gly Asn Gly Gln Gly His Asn Met Pro Asn Asp Pro Asn Arg Asn Val 
                85                  90                  95      


Asp Glu Asn Ala Asn Ala Asn Ser Ala Val Lys Asn Asn Asn Asn Glu 
            100                 105                 110         


Glu Pro Ser Asp Lys His Ile Lys Glu Tyr Leu Asn Lys Ile Gln Asn 
        115                 120                 125             


Ser Leu Ser Thr Glu Trp Ser Pro Cys Ser Val Thr Cys Gly Asn Gly 
    130                 135                 140                 


Ile Gln Val Arg Ile Lys Pro Gly Ser Ala Asn Lys Pro Lys Asp Glu 
145                 150                 155                 160 


Leu Asp Tyr Ala Asn Asp Ile Glu Lys Lys Ile Cys Lys Met Glu Lys 
                165                 170                 175     


Cys Ser Ser Val Pro Val Thr Asn Met Glu Asn Ile Thr Ser Gly Phe 
            180                 185                 190         


Leu Gly Pro Leu Leu Val Leu Gln Ala Gly Phe Phe Leu Leu Thr Arg 
        195                 200                 205             


Ile Leu Thr Ile Pro Gln Ser Leu Asp Ser Trp Trp Thr Ser Leu Asn 
    210                 215                 220                 


Phe Leu Gly Gly Ser Pro Val Cys Leu Gly Gln Asn Ser Gln Ser Pro 
225                 230                 235                 240 


Thr Ser Asn His Ser Pro Thr Ser Cys Pro Pro Ile Cys Pro Gly Tyr 
                245                 250                 255     


Arg Trp Met Cys Leu Arg Arg Phe Ile Ile Phe Leu Phe Ile Leu Leu 
            260                 265                 270         


Leu Cys Leu Ile Phe Leu Leu Val Leu Leu Asp Tyr Gln Gly Met Leu 
        275                 280                 285             


Pro Val Cys Pro Leu Ile Pro Gly Ser Thr Thr Thr Asn Thr Gly Pro 
    290                 295                 300                 


Cys Lys Thr Cys Thr Thr Pro Ala Gln Gly Asn Ser Met Phe Pro Ser 
305                 310                 315                 320 


Cys Cys Cys Thr Lys Pro Thr Asp Gly Asn Cys Thr Cys Ile Pro Ile 
                325                 330                 335     


Pro Ser Ser Trp Ala Phe Ala Lys Tyr Leu Trp Glu Trp Ala Ser Val 
            340                 345                 350         


Arg Phe Ser Trp Leu Ser Leu Leu Val Pro Phe Val Gln Trp Phe Val 
        355                 360                 365             


Gly Leu Ser Pro Thr Val Trp Leu Ser Ala Ile Trp Met Met Trp Tyr 
    370                 375                 380                 


Trp Gly Pro Ser Leu Tyr Ser Ile Val Ser Pro Phe Ile Pro Leu Leu 
385                 390                 395                 400 


Pro Ile Phe Phe Cys Leu Trp Val Tyr Ile 
                405                 410 


<210>  2
<211>  414
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  R21C (R21 with C-terminal EPEA tag)

<400>  2

Met Asp Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
1               5                   10                  15      


Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
            20                  25                  30          


Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
        35                  40                  45              


Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn 
    50                  55                  60                  


Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Lys Asn Asn Gln 
65                  70                  75                  80  


Gly Asn Gly Gln Gly His Asn Met Pro Asn Asp Pro Asn Arg Asn Val 
                85                  90                  95      


Asp Glu Asn Ala Asn Ala Asn Ser Ala Val Lys Asn Asn Asn Asn Glu 
            100                 105                 110         


Glu Pro Ser Asp Lys His Ile Lys Glu Tyr Leu Asn Lys Ile Gln Asn 
        115                 120                 125             


Ser Leu Ser Thr Glu Trp Ser Pro Cys Ser Val Thr Cys Gly Asn Gly 
    130                 135                 140                 


Ile Gln Val Arg Ile Lys Pro Gly Ser Ala Asn Lys Pro Lys Asp Glu 
145                 150                 155                 160 


Leu Asp Tyr Ala Asn Asp Ile Glu Lys Lys Ile Cys Lys Met Glu Lys 
                165                 170                 175     


Cys Ser Ser Val Pro Val Thr Asn Met Glu Asn Ile Thr Ser Gly Phe 
            180                 185                 190         


Leu Gly Pro Leu Leu Val Leu Gln Ala Gly Phe Phe Leu Leu Thr Arg 
        195                 200                 205             


Ile Leu Thr Ile Pro Gln Ser Leu Asp Ser Trp Trp Thr Ser Leu Asn 
    210                 215                 220                 


Phe Leu Gly Gly Ser Pro Val Cys Leu Gly Gln Asn Ser Gln Ser Pro 
225                 230                 235                 240 


Thr Ser Asn His Ser Pro Thr Ser Cys Pro Pro Ile Cys Pro Gly Tyr 
                245                 250                 255     


Arg Trp Met Cys Leu Arg Arg Phe Ile Ile Phe Leu Phe Ile Leu Leu 
            260                 265                 270         


Leu Cys Leu Ile Phe Leu Leu Val Leu Leu Asp Tyr Gln Gly Met Leu 
        275                 280                 285             


Pro Val Cys Pro Leu Ile Pro Gly Ser Thr Thr Thr Asn Thr Gly Pro 
    290                 295                 300                 


Cys Lys Thr Cys Thr Thr Pro Ala Gln Gly Asn Ser Met Phe Pro Ser 
305                 310                 315                 320 


Cys Cys Cys Thr Lys Pro Thr Asp Gly Asn Cys Thr Cys Ile Pro Ile 
                325                 330                 335     


Pro Ser Ser Trp Ala Phe Ala Lys Tyr Leu Trp Glu Trp Ala Ser Val 
            340                 345                 350         


Arg Phe Ser Trp Leu Ser Leu Leu Val Pro Phe Val Gln Trp Phe Val 
        355                 360                 365             


Gly Leu Ser Pro Thr Val Trp Leu Ser Ala Ile Trp Met Met Trp Tyr 
    370                 375                 380                 


Trp Gly Pro Ser Leu Tyr Ser Ile Val Ser Pro Phe Ile Pro Leu Leu 
385                 390                 395                 400 


Pro Ile Phe Phe Cys Leu Trp Val Tyr Ile Glu Pro Glu Ala 
                405                 410                 


<210>  3
<211>  492
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RV21

<400>  3

Gly Asp Arg Ala Ala Gly Gln Pro Ala Gly Asp Arg Ala Ala Gly Gln 
1               5                   10                  15      


Pro Ala Gly Asp Arg Ala Ala Gly Gln Pro Ala Gly Asp Arg Ala Ala 
            20                  25                  30          


Gly Gln Pro Ala Gly Asp Arg Ala Ala Gly Gln Pro Ala Gly Asp Arg 
        35                  40                  45              


Ala Ala Gly Gln Pro Ala Gly Asp Arg Ala Ala Gly Gln Pro Ala Gly 
    50                  55                  60                  


Asp Arg Ala Asp Gly Gln Pro Ala Gly Asp Arg Ala Asp Gly Gln Pro 
65                  70                  75                  80  


Ala Gly Asn Gly Ala Gly Gly Gln Ala Ala Ala Asn Gly Ala Gly Asn 
                85                  90                  95      


Gln Pro Gly Ala Asn Gly Ala Gly Gly Gln Ala Ala Ala Asn Gly Ala 
            100                 105                 110         


Gly Asn Gln Pro Gly Ala Asn Gly Ala Gly Gly Gln Ala Ala Ala Asn 
        115                 120                 125             


Gly Ala Gly Asp Gln Pro Gly Ala Asn Gly Ala Gly Asp Gln Pro Gly 
    130                 135                 140                 


Ala Asn Gly Ala Asp Asp Gln Pro Gly Ala Asn Gly Ala Gly Asp Gln 
145                 150                 155                 160 


Pro Gly Glu Asp Gly Ala Gly Asn Gln Pro Gly Ala Asn Gly Ala Gly 
                165                 170                 175     


Asp Gln Pro Gly Gln Ala Ala Gly Gly Asn Ala Gly Gly Gly Gln Gly 
            180                 185                 190         


Gln Asn Asn Glu Gly Ala Asn Ala Pro Asn Glu Lys Ser Val Lys Glu 
        195                 200                 205             


Tyr Leu Asp Lys Val Arg Ala Thr Val Gly Thr Glu Trp Thr Pro Cys 
    210                 215                 220                 


Ser Val Thr Cys Gly Val Gly Val Arg Val Arg Arg Arg Val Asn Ala 
225                 230                 235                 240 


Ala Asn Lys Lys Pro Glu Asp Leu Thr Leu Asn Asp Leu Glu Thr Asp 
                245                 250                 255     


Val Cys Thr Met Asp Lys Pro Val Thr Asn Met Glu Asn Ile Thr Ser 
            260                 265                 270         


Gly Phe Leu Gly Pro Leu Leu Val Leu Gln Ala Gly Phe Phe Leu Leu 
        275                 280                 285             


Thr Arg Ile Leu Thr Ile Pro Gln Ser Leu Asp Ser Trp Trp Thr Ser 
    290                 295                 300                 


Leu Asn Phe Leu Gly Gly Ser Pro Val Cys Leu Gly Gln Asn Ser Gln 
305                 310                 315                 320 


Ser Pro Thr Ser Asn His Ser Pro Thr Ser Cys Pro Pro Ile Cys Pro 
                325                 330                 335     


Gly Tyr Arg Trp Met Cys Leu Arg Arg Phe Ile Ile Phe Leu Phe Ile 
            340                 345                 350         


Leu Leu Leu Cys Leu Ile Phe Leu Leu Val Leu Leu Asp Tyr Gln Gly 
        355                 360                 365             


Met Leu Pro Val Cys Pro Leu Ile Pro Gly Ser Thr Thr Thr Asn Thr 
    370                 375                 380                 


Gly Pro Cys Lys Thr Cys Thr Thr Pro Ala Gln Gly Asn Ser Met Phe 
385                 390                 395                 400 


Pro Ser Cys Cys Cys Thr Lys Pro Thr Asp Gly Asn Cys Thr Cys Ile 
                405                 410                 415     


Pro Ile Pro Ser Ser Trp Ala Phe Ala Lys Tyr Leu Trp Glu Trp Ala 
            420                 425                 430         


Ser Val Arg Phe Ser Trp Leu Ser Leu Leu Val Pro Phe Val Gln Trp 
        435                 440                 445             


Phe Val Gly Leu Ser Pro Thr Val Trp Leu Ser Ala Ile Trp Met Met 
    450                 455                 460                 


Trp Tyr Trp Gly Pro Ser Leu Tyr Ser Ile Val Ser Pro Phe Ile Pro 
465                 470                 475                 480 


Leu Leu Pro Ile Phe Phe Cys Leu Trp Val Tyr Ile 
                485                 490         


<210>  4
<211>  496
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RV21 with C-Terminal EPEA tag

<400>  4

Gly Asp Arg Ala Ala Gly Gln Pro Ala Gly Asp Arg Ala Ala Gly Gln 
1               5                   10                  15      


Pro Ala Gly Asp Arg Ala Ala Gly Gln Pro Ala Gly Asp Arg Ala Ala 
            20                  25                  30          


Gly Gln Pro Ala Gly Asp Arg Ala Ala Gly Gln Pro Ala Gly Asp Arg 
        35                  40                  45              


Ala Ala Gly Gln Pro Ala Gly Asp Arg Ala Ala Gly Gln Pro Ala Gly 
    50                  55                  60                  


Asp Arg Ala Asp Gly Gln Pro Ala Gly Asp Arg Ala Asp Gly Gln Pro 
65                  70                  75                  80  


Ala Gly Asn Gly Ala Gly Gly Gln Ala Ala Ala Asn Gly Ala Gly Asn 
                85                  90                  95      


Gln Pro Gly Ala Asn Gly Ala Gly Gly Gln Ala Ala Ala Asn Gly Ala 
            100                 105                 110         


Gly Asn Gln Pro Gly Ala Asn Gly Ala Gly Gly Gln Ala Ala Ala Asn 
        115                 120                 125             


Gly Ala Gly Asp Gln Pro Gly Ala Asn Gly Ala Gly Asp Gln Pro Gly 
    130                 135                 140                 


Ala Asn Gly Ala Asp Asp Gln Pro Gly Ala Asn Gly Ala Gly Asp Gln 
145                 150                 155                 160 


Pro Gly Glu Asp Gly Ala Gly Asn Gln Pro Gly Ala Asn Gly Ala Gly 
                165                 170                 175     


Asp Gln Pro Gly Gln Ala Ala Gly Gly Asn Ala Gly Gly Gly Gln Gly 
            180                 185                 190         


Gln Asn Asn Glu Gly Ala Asn Ala Pro Asn Glu Lys Ser Val Lys Glu 
        195                 200                 205             


Tyr Leu Asp Lys Val Arg Ala Thr Val Gly Thr Glu Trp Thr Pro Cys 
    210                 215                 220                 


Ser Val Thr Cys Gly Val Gly Val Arg Val Arg Arg Arg Val Asn Ala 
225                 230                 235                 240 


Ala Asn Lys Lys Pro Glu Asp Leu Thr Leu Asn Asp Leu Glu Thr Asp 
                245                 250                 255     


Val Cys Thr Met Asp Lys Pro Val Thr Asn Met Glu Asn Ile Thr Ser 
            260                 265                 270         


Gly Phe Leu Gly Pro Leu Leu Val Leu Gln Ala Gly Phe Phe Leu Leu 
        275                 280                 285             


Thr Arg Ile Leu Thr Ile Pro Gln Ser Leu Asp Ser Trp Trp Thr Ser 
    290                 295                 300                 


Leu Asn Phe Leu Gly Gly Ser Pro Val Cys Leu Gly Gln Asn Ser Gln 
305                 310                 315                 320 


Ser Pro Thr Ser Asn His Ser Pro Thr Ser Cys Pro Pro Ile Cys Pro 
                325                 330                 335     


Gly Tyr Arg Trp Met Cys Leu Arg Arg Phe Ile Ile Phe Leu Phe Ile 
            340                 345                 350         


Leu Leu Leu Cys Leu Ile Phe Leu Leu Val Leu Leu Asp Tyr Gln Gly 
        355                 360                 365             


Met Leu Pro Val Cys Pro Leu Ile Pro Gly Ser Thr Thr Thr Asn Thr 
    370                 375                 380                 


Gly Pro Cys Lys Thr Cys Thr Thr Pro Ala Gln Gly Asn Ser Met Phe 
385                 390                 395                 400 


Pro Ser Cys Cys Cys Thr Lys Pro Thr Asp Gly Asn Cys Thr Cys Ile 
                405                 410                 415     


Pro Ile Pro Ser Ser Trp Ala Phe Ala Lys Tyr Leu Trp Glu Trp Ala 
            420                 425                 430         


Ser Val Arg Phe Ser Trp Leu Ser Leu Leu Val Pro Phe Val Gln Trp 
        435                 440                 445             


Phe Val Gly Leu Ser Pro Thr Val Trp Leu Ser Ala Ile Trp Met Met 
    450                 455                 460                 


Trp Tyr Trp Gly Pro Ser Leu Tyr Ser Ile Val Ser Pro Phe Ile Pro 
465                 470                 475                 480 


Leu Leu Pro Ile Phe Phe Cys Leu Trp Val Tyr Ile Glu Pro Glu Ala 
                485                 490                 495     


<210>  5
<211>  424
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RTSS  (11 C-terminal amino acids more than R21 plus 3 extra at 
       N-terminus)

<400>  5

Met Met Ala Pro Asp Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
1               5                   10                  15      


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
            20                  25                  30          


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
        35                  40                  45              


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala 
    50                  55                  60                  


Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Ala Asn Pro Asn Lys 
65                  70                  75                  80  


Asn Asn Gln Gly Asn Gly Gln Gly His Asn Met Pro Asn Asp Pro Asn 
                85                  90                  95      


Arg Asn Val Asp Glu Asn Ala Asn Ala Asn Ser Ala Val Lys Asn Asn 
            100                 105                 110         


Asn Asn Glu Glu Pro Ser Asp Lys His Ile Lys Glu Tyr Leu Asn Lys 
        115                 120                 125             


Ile Gln Asn Ser Leu Ser Thr Glu Trp Ser Pro Cys Ser Val Thr Cys 
    130                 135                 140                 


Gly Asn Gly Ile Gln Val Arg Ile Lys Pro Gly Ser Ala Asn Lys Pro 
145                 150                 155                 160 


Lys Asp Glu Leu Asp Tyr Ala Asn Asp Ile Glu Lys Lys Ile Cys Lys 
                165                 170                 175     


Met Glu Lys Cys Ser Ser Val Phe Asn Val Val Asn Ser Ser Ile Gly 
            180                 185                 190         


Leu Gly Pro Val Thr Asn Met Glu Asn Ile Thr Ser Gly Phe Leu Gly 
        195                 200                 205             


Pro Leu Leu Val Leu Gln Ala Gly Phe Phe Leu Leu Thr Arg Ile Leu 
    210                 215                 220                 


Thr Leu Pro Gln Ser Leu Asp Ser Trp Trp Thr Ser Leu Asn Phe Leu 
225                 230                 235                 240 


Gly Gly Ser Pro Val Cys Leu Gly Gln Asn Ser Gln Ser Pro Thr Ser 
                245                 250                 255     


Asn His Ser Pro Thr Ser Cys Pro Pro Ile Cys Pro Gly Tyr Arg Trp 
            260                 265                 270         


Met Cys Leu Arg Arg Phe Ile Ile Phe Leu Phe Ile Leu Leu Leu Cys 
        275                 280                 285             


Leu Ile Phe Leu Leu Val Leu Leu Asp Tyr Gln Gly Met Leu Pro Val 
    290                 295                 300                 


Cys Pro Leu Ile Pro Gly Ser Thr Thr Thr Asn Thr Gly Pro Cys Lys 
305                 310                 315                 320 


Thr Cys Thr Thr Pro Ala Gln Gly Asn Ser Met Phe Pro Ser Cys Cys 
                325                 330                 335     


Cys Thr Lys Pro Thr Asp Gly Asn Cys Thr Cys Ile Pro Ile Pro Ser 
            340                 345                 350         


Ser Trp Ala Phe Ala Lys Tyr Leu Trp Glu Trp Ala Ser Val Arg Phe 
        355                 360                 365             


Ser Trp Leu Ser Leu Leu Val Pro Phe Val Gln Trp Phe Val Gly Leu 
    370                 375                 380                 


Ser Pro Thr Val Trp Leu Ser Ala Ile Trp Met Met Trp Tyr Trp Gly 
385                 390                 395                 400 


Pro Ser Leu Tyr Ser Ile Val Ser Pro Phe Ile Pro Leu Leu Pro Ile 
                405                 410                 415     


Phe Phe Cys Leu Trp Val Tyr Ile 
            420                 


<210>  6
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  internal repeat

<400>  6

Asn Ala Asn Pro 
1               


<210>  7
<211>  115
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CS sequence

<400>  7

Asn Lys Asn Asn Gln Gly Asn Gly Gln Gly His Asn Met Pro Asn Asp 
1               5                   10                  15      


Pro Asn Arg Asn Val Asp Glu Asn Ala Asn Ala Asn Ser Ala Val Lys 
            20                  25                  30          


Asn Asn Asn Asn Glu Glu Pro Ser Asp Lys His Ile Lys Glu Tyr Leu 
        35                  40                  45              


Asn Lys Ile Gln Asn Ser Leu Ser Thr Glu Trp Ser Pro Cys Ser Val 
    50                  55                  60                  


Thr Cys Gly Asn Gly Ile Gln Val Arg Ile Lys Pro Gly Ser Ala Asn 
65                  70                  75                  80  


Lys Pro Lys Asp Glu Leu Asp Tyr Ala Asn Asp Ile Glu Lys Lys Ile 
                85                  90                  95      


Cys Lys Met Glu Lys Cys Ser Ser Val Phe Asn Val Val Asn Ser Ser 
            100                 105                 110         


Ile Gly Ile 
        115 


<210>  8
<211>  102
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CS sequence

<400>  8

Asn Lys Asn Gln Gly Asn Gly Gln Gly His Asn Met Pro Asn Asp Pro 
1               5                   10                  15      


Asn Arg Asn Val Asp Glu Asn Ala Asn Ala Asn Ser Ala Val Lys Asn 
            20                  25                  30          


Asn Glu Glu Pro Ser Asp Lys His Ile Lys Glu Tyr Leu Asn Lys Ile 
        35                  40                  45              


Gln Asn Ser Leu Ser Thr Glu Trp Ser Pro Cys Ser Val Thr Cys Gly 
    50                  55                  60                  


Asn Gly Ile Gln Val Arg Ile Lys Pro Gly Ser Ala Asn Lys Pro Lys 
65                  70                  75                  80  


Asp Glu Leu Asp Tyr Ala Asn Asp Ile Glu Lys Lys Ile Cys Lys Met 
                85                  90                  95      


Glu Lys Cys Ser Ser Val 
            100         


