               SEQUENCE LISTING

<110> Medizinische Universitaet Wien

<120> Superactive mutant thymidine kinase for use in cancer therapy

<130> Z2534 PCT S3

<150> EP17 18 3361.9
<151> 2017-07-26

<160> 53

<170> BiSSAP 1.3.6

<210> 1
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutM73AA75DD83AG9095A

<400> 1
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccgccg aggacctgcc cgcctgcctg     240

ctccgagccg tggcccagga ggccctggct gtggctgtca tagctatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 2
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutM73AA75DD83AG9095A

<400> 2
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Ala Glu Asp Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Ala Val Ala Gln Glu Ala Leu Ala Val Ala Val Ile Ala Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 3
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutM73AA75DD83AG90A

<400> 3
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccgccg aggacctgcc cgcctgcctg     240

ctccgagccg tggcccagga ggccctggct gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 4
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutM73AA75DD83AG90A

<400> 4
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Ala Glu Asp Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Ala Val Ala Gln Glu Ala Leu Ala Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 5
<211> 702
<212> RNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutV84EG90A

<400> 5
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccatgg aggcgctgcc cgcctgcctg     240

ctccgagacg aggcccagga ggccctggct gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 6
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutV84EG90A

<400> 6
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Met Glu Ala Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Glu Ala Gln Glu Ala Leu Ala Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 7
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutM73AA75DG9095A

<400> 7
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccgccg aggacctgcc cgcctgcctg     240

ctccgagacg tggcccagga ggccctggct gtggctgtca tagctatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 8
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutM73AA75DG9095A

<400> 8
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Ala Glu Asp Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Val Ala Gln Glu Ala Leu Ala Val Ala Val Ile Ala Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 9
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutM73AA75DG90A

<400> 9
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccgccg aggacctgcc cgcctgcctg     240

ctccgagacg tggcccagga ggccctggct gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 10
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutM73AA75DG90A

<400> 10
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Ala Glu Asp Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Val Ala Gln Glu Ala Leu Ala Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 11
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutG90A

<400> 11
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccatgg aggcgctgcc cgcctgcctg     240

ctccgagacg tggcccagga ggccctggct gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 12
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutG90A

<400> 12
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Met Glu Ala Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Val Ala Gln Glu Ala Leu Ala Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 13
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutA75DG90E

<400> 13
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccatgg aggacctgcc cgcctgcctg     240

ctccgagacg tggcccagga ggccctggag gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 14
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutA75DG90E

<400> 14
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Met Glu Asp Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Val Ala Gln Glu Ala Leu Glu Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 15
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutV84DG90A

<400> 15
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccatgg aggcgctgcc cgcctgcctg     240

ctccgagacg acgcccagga ggccctggct gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 16
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutV84DG90A

<400> 16
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Met Glu Ala Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Asp Ala Gln Glu Ala Leu Ala Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 17
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutA75GA84EG90A

<400> 17
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccatgg agggcctgcc cgcctgcctg     240

ctccgagacg aggcccagga ggccctggct gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 18
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutA75GV84EG90A

<400> 18
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Met Glu Gly Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Glu Ala Gln Glu Ala Leu Ala Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 19
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutG90D

<400> 19
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccatgg aggcgctgcc cgcctgcctg     240

ctccgagacg tggcccagga ggccctggac gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 20
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutG90D

<400> 20
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Met Glu Ala Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Val Ala Gln Glu Ala Leu Asp Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 21
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutG90V

<400> 21
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccatgg aggcgctgcc cgcctgcctg     240

ctccgagacg tggcccagga ggccctggtg gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 22
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutG90V

<400> 22
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Met Glu Ala Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Val Ala Gln Glu Ala Leu Val Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 23
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutA75QV84E

<400> 23
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccatgg agcagctgcc cgcctgcctg     240

ctccgagacg aggcccagga ggccctgggc gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 24
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutA75QV84E

<400> 24
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Met Glu Gln Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Glu Ala Gln Glu Ala Leu Gly Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 25
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mutL8081F

<400> 25
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccatgg aggcactgcc cgcctgcttc     240

ttccgagacg tggcccagga ggccctgggc gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 26
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> Human TK1 mutL8081F

<400> 26
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Met Glu Ala Leu Pro Ala Cys Phe 
65                  70                  75                  80  
Phe Arg Asp Val Ala Gln Glu Ala Leu Gly Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Arg Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 27
<211> 705
<212> DNA
<213> Homo sapiens


<220> 
<223> Human TK1 wt NM_003258 (ORF)

<400> 27
atgagctgca ttaacctgcc cactgtgctg cctggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccatgg aggcactgcc cgcctgcctg     240

ctccgagacg tggcccagga ggccctgggc gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aagccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgc agccctgcca actga                     705


<210> 28
<211> 234
<212> PRT
<213> Homo sapiens


<220> 
<223> Human TK1 wt NM_003258

<400> 28
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Met Glu Ala Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Val Ala Gln Glu Ala Leu Gly Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Lys Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Cys Ser Pro Ala Asn 
225                 230                 

<210> 29
<211> 1616
<212> DNA
<213> Homo sapiens


<400> 29
gcgcacgtcc cggattcctc ccacgagggg gcgggctgcg gccaaatctc ccgccaggtc      60

agcggccggg cgctgattgg ccccatggcg gcggggccgg ctcgtgattg gccagcacgc     120

cgtggtttaa agcggtcggc gcgggaacca ggggcttact gcgggacggc cttggagagt     180

actcgggttc gtgaacttcc cggaggcgca atgagctgca ttaacctgcc cactgtgctg     240

cctggctccc ccagcaagac ccgggggcag atccaggtga ttctcgggcc gatgttctca     300

ggaaaaagca cagagttgat gagacgcgtc cgtcgcttcc agattgctca gtacaagtgc     360

ctggtgatca agtatgccaa agacactcgc tacagcagca gcttctgcac acatgaccgg     420

aacaccatgg aggcactgcc cgcctgcctg ctccgagacg tggcccagga ggccctgggc     480

gtggctgtca taggcatcga cgaggggcag tttttccctg acatcgtgga gttctgcgag     540

gccatggcca acgccgggaa gaccgtaatt gtggctgcac tggatgggac cttccagagg     600

aagccatttg gggccatcct gaacctggtg ccgctggccg agagcgtggt gaagctgacg     660

gcggtgtgca tggagtgctt ccgggaagcc gcctatacca agaggctcgg cacagagaag     720

gaggtcgagg tgattggggg agcagacaag taccactccg tgtgtcggct ctgctacttc     780

aagaaggcct caggccagcc tgccgggccg gacaacaaag agaactgccc agtgccagga     840

aagccagggg aagccgtggc tgccaggaag ctctttgccc cacagcagat tctgcaatgc     900

agccctgcca actgagggac ctgcgagggc cgcccgctcc cttcctgcca ctgccgccta     960

ctggacgctg ccctgcatgc tgcccagcca ctccaggagg aagtcgggag gcgtggaggg    1020

tgaccacacc ttggccttct gggaactctc ctttgtgtgg ctgccccacc tgccgcatgc    1080

tccctcctct cctacccact ggtctgctta aagcttccct ctcagctgct gggacgatcg    1140

cccaggctgg agctggcccc gcttggtggc ctgggatctg gcacactccc tctccttggg    1200

gtgagggaca gagccccacg ctgttgacat cagcctgctt cttcccctct gcggctttca    1260

ctgctgagtt tctgttctcc ctgggaagcc tgtgccagca cctttgagcc ttggcccaca    1320

ctgaggctta ggcctctctg cctgggatgg gctcccaccc tcccctgagg atggcctgga    1380

ttcacgccct cttgtttcct tttgggctca aagcccttcc tacctctggt gatggtttcc    1440

acaggaacaa cagcatcttt caccaagatg ggtggcacca accttgctgg gacttggatc    1500

ccaggggctt atctcttcaa gtgtggagag ggcagggtcc acgcctctgc tgtagcttat    1560

gaaattaact aattgaaaat tcactggttg gtggaaaaaa aaaaaaaaaa aaaaaa        1616


<210> 30
<211> 702
<212> DNA
<213> Artificial Sequence


<220> 
<223> Human TK1 mut G90A

<400> 30
atgagctgca ttaacctgcc cactgtgctg cccggctccc ccagcaagac ccgggggcag      60

atccaggtga ttctcgggcc gatgttctca ggaaaaagca cagagttgat gagacgcgtc     120

cgtcgcttcc agattgctca gtacaagtgc ctggtgatca agtatgccaa agacactcgc     180

tacagcagca gcttctgcac acatgaccgg aacaccatgg aggcgctgcc cgcctgcctg     240

ctccgagacg tggcccagga ggccctggct gtggctgtca taggcatcga cgaggggcag     300

tttttccctg acatcgtgga gttctgcgag gccatggcca acgccgggaa gaccgtaatt     360

gtggctgcac tggatgggac cttccagagg aagccatttg gggccatcct gaacctggtg     420

ccgctggccg agagcgtggt gaagctgacg gcggtgtgca tggagtgctt ccgggaagcc     480

gcctatacca agaggctcgg cacagagaag gaggtcgagg tgattggggg agcagacaag     540

taccactccg tgtgtcggct ctgctacttc aagaaggcct caggccagcc tgccgggccg     600

gacaacaaag agaactgccc agtgccagga aggccagggg aagccgtggc tgccaggaag     660

ctctttgccc cacagcagat tctgcaatgg atccagacat ga                        702


<210> 31
<211> 233
<212> PRT
<213> Artificial Sequence


<220> 
<223> mutant human thymidine kinase

<400> 31
Met Ser Cys Ile Asn Leu Pro Thr Val Leu Pro Gly Ser Pro Ser Lys 
1               5                   10                  15      
Thr Arg Gly Gln Ile Gln Val Ile Leu Gly Pro Met Phe Ser Gly Lys 
            20                  25                  30          
Ser Thr Glu Leu Met Arg Arg Val Arg Arg Phe Gln Ile Ala Gln Tyr 
        35                  40                  45              
Lys Cys Leu Val Ile Lys Tyr Ala Lys Asp Thr Arg Tyr Ser Ser Ser 
    50                  55                  60                  
Phe Cys Thr His Asp Arg Asn Thr Met Glu Ala Leu Pro Ala Cys Leu 
65                  70                  75                  80  
Leu Arg Asp Val Ala Gln Glu Ala Leu Ala Val Ala Val Ile Gly Ile 
                85                  90                  95      
Asp Glu Gly Gln Phe Phe Pro Asp Ile Val Glu Phe Cys Glu Ala Met 
            100                 105                 110         
Ala Asn Ala Gly Lys Thr Val Ile Val Ala Ala Leu Asp Gly Thr Phe 
        115                 120                 125             
Gln Arg Lys Pro Phe Gly Ala Ile Leu Asn Leu Val Pro Leu Ala Glu 
    130                 135                 140                 
Ser Val Val Lys Leu Thr Ala Val Cys Met Glu Cys Phe Arg Glu Ala 
145                 150                 155                 160 
Ala Tyr Thr Lys Arg Leu Gly Thr Glu Lys Glu Val Glu Val Ile Gly 
                165                 170                 175     
Gly Ala Asp Lys Tyr His Ser Val Cys Arg Leu Cys Tyr Phe Lys Lys 
            180                 185                 190         
Ala Ser Gly Gln Pro Ala Gly Pro Asp Asn Lys Glu Asn Cys Pro Val 
        195                 200                 205             
Pro Gly Lys Pro Gly Glu Ala Val Ala Ala Arg Lys Leu Phe Ala Pro 
    210                 215                 220                 
Gln Gln Ile Leu Gln Trp Ile Gln Thr 
225                 230             

<210> 32
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human wtTk1

<400> 32
Asn Thr Met Glu Ala Leu Pro Ala Cys Leu Leu Arg Asp Val Ala Gln 
1               5                   10                  15      
Glu Ala Leu Gly Val Ala Val Ile Gly 
            20                  25  

<210> 33
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK1 mutM73AA75DD83AG9095A

<400> 33
Asn Thr Ala Glu Asp Leu Pro Ala Cys Leu Leu Arg Ala Val Ala Gln 
1               5                   10                  15      
Glu Ala Leu Ala Val Ala Val Ile Ala 
            20                  25  

<210> 34
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK1 mutM73AA75DD83AG90A

<400> 34
Asn Thr Ala Glu Asp Leu Pro Ala Cys Leu Leu Arg Ala Val Ala Gln 
1               5                   10                  15      
Glu Ala Leu Ala Val Ala Val Ile Gly 
            20                  25  

<210> 35
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK1 mut V84EG90A

<400> 35
Asn Thr Met Glu Ala Leu Pro Ala Cys Leu Leu Arg Asp Glu Ala Gln 
1               5                   10                  15      
Glu Ala Leu Ala Val Ala Val Ile Gly 
            20                  25  

<210> 36
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK1 mutM73AA75DG9095A

<400> 36
Asn Thr Ala Glu Asp Leu Pro Ala Cys Leu Leu Arg Asp Val Ala Gln 
1               5                   10                  15      
Glu Ala Leu Ala Val Ala Val Ile Ala 
            20                  25  

<210> 37
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK1 mutM73AA75DG90A

<400> 37
Asn Thr Ala Glu Asp Leu Pro Ala Cys Leu Leu Arg Asp Val Ala Gln 
1               5                   10                  15      
Glu Ala Leu Ala Val Ala Val Ile Gly 
            20                  25  

<210> 38
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK1 mut G90A

<400> 38
Asn Thr Met Glu Ala Leu Pro Ala Cys Leu Leu Arg Asp Val Ala Gln 
1               5                   10                  15      
Glu Ala Leu Ala Val Ala Val Ile Gly 
            20                  25  

<210> 39
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK 1 mut A75DG90E

<400> 39
Asn Thr Met Glu Asp Leu Pro Ala Cys Leu Leu Arg Asp Val Ala Gln 
1               5                   10                  15      
Glu Ala Leu Glu Val Ala Val Ile Gly 
            20                  25  

<210> 40
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK1 mut V84DG90A

<400> 40
Asn Thr Met Glu Ala Leu Pro Ala Cys Leu Leu Arg Asp Asp Ala Gln 
1               5                   10                  15      
Glu Ala Leu Ala Val Ala Val Ile Gly 
            20                  25  

<210> 41
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK 1 mut A75GV84EG90A

<400> 41
Asn Thr Met Glu Gly Leu Pro Ala Cys Leu Leu Arg Asp Glu Ala Gln 
1               5                   10                  15      
Glu Ala Leu Ala Val Ala Val Ile Gly 
            20                  25  

<210> 42
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK1 mut G90D

<400> 42
Asn Thr Met Glu Ala Leu Pro Ala Cys Leu Leu Arg Asp Val Ala Gln 
1               5                   10                  15      
Glu Ala Leu Asp Val Ala Val Ile Gly 
            20                  25  

<210> 43
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK1 mut G90V

<400> 43
Asn Thr Met Glu Ala Leu Pro Ala Cys Leu Leu Arg Asp Val Ala Gln 
1               5                   10                  15      
Glu Ala Leu Val Val Ala Val Ile Gly 
            20                  25  

<210> 44
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK 1 mut A75QV84E

<400> 44
Asn Thr Met Glu Gln Leu Pro Ala Cys Leu Leu Arg Asp Glu Ala Gln 
1               5                   10                  15      
Glu Ala Leu Gly Val Ala Val Ile Gly 
            20                  25  

<210> 45
<211> 25
<212> PRT
<213> Artificial Sequence


<220> 
<223> human TK1 mut L8081F

<400> 45
Asn Thr Met Glu Ala Leu Pro Ala Cys Phe Phe Arg Asp Val Ala Gln 
1               5                   10                  15      
Glu Ala Leu Gly Val Ala Val Ile Gly 
            20                  25  

<210> 46
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> superTK1 antisense (pUHDhTk1as)

<400> 46
attaacctgc ccactgtgct                                                20


<210> 47
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> superTK1 sense (pUHDhumTK1s)

<400> 47
ctgtggggca aagagcttc                                                 19


<210> 48
<211> 19
<212> DNA
<213> Artificial Sequence


<220> 
<223> superTK1 sense (pUHDhumTK1s)

<400> 48
ctgtgggcca aagagcttc                                                 19


<210> 49
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> hygromycin sense

<400> 49
gatgtaggag ggcgtggata                                                20


<210> 50
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> hygromycin antisense

<400> 50
ataggtcagg ctctcgctga                                                20


<210> 51
<211> 18
<212> DNA
<213> Artificial Sequence


<220> 
<223> nucleotide sequence encoding CSPAN

<400> 51
tgcagccctg ccaactga                                                  18


<210> 52
<211> 5
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminus of wt tymidine kinase

<400> 52
Cys Ser Pro Ala Asn 
1               5   

<210> 53
<211> 4
<212> PRT
<213> Artificial Sequence


<220> 
<223> C-terminus of mutant tymidine kinase

<400> 53
Trp Ile Gln Thr 
1               

