                         SEQUENCE LISTING

<110>  Roche Diagnostics GmbH
       F. Hoffmann-La Roche AG
       Roche Diagnostics Operations, Inc.
 
<120>  Multi-epitope fusion protein of an HCV antigen and uses thereof

<130>  P34291-WO-IR

<150>  EP17183523.4
<151>  2017-07-27

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  191
<212>  PRT
<213>  Hepatitis C virus

<400>  1

Met Ser Thr Asn Pro Lys Pro Gln Lys Lys Asn Lys Arg Asn Thr Asn 
1               5                   10                  15      


Arg Arg Pro Gln Asp Val Lys Phe Pro Gly Gly Gly Gln Ile Val Gly 
            20                  25                  30          


Gly Val Tyr Leu Leu Pro Arg Arg Gly Pro Arg Leu Gly Val Arg Ala 
        35                  40                  45              


Thr Arg Lys Thr Ser Glu Arg Ser Gln Pro Arg Gly Arg Arg Gln Pro 
    50                  55                  60                  


Ile Pro Lys Ala Arg Arg Pro Glu Gly Arg Thr Trp Ala Gln Pro Gly 
65                  70                  75                  80  


Tyr Pro Trp Pro Leu Tyr Gly Asn Glu Gly Cys Gly Trp Ala Gly Trp 
                85                  90                  95      


Leu Leu Ser Pro Arg Gly Ser Arg Pro Ser Trp Gly Pro Thr Asp Pro 
            100                 105                 110         


Arg Arg Arg Ser Arg Asn Leu Gly Lys Val Ile Asp Thr Leu Thr Cys 
        115                 120                 125             


Gly Phe Ala Asp Leu Met Gly Tyr Ile Pro Leu Val Gly Ala Pro Leu 
    130                 135                 140                 


Gly Gly Ala Ala Arg Ala Leu Ala His Gly Val Arg Val Leu Glu Asp 
145                 150                 155                 160 


Gly Val Asn Tyr Ala Thr Gly Asn Leu Pro Gly Cys Ser Phe Ser Ile 
                165                 170                 175     


Phe Leu Leu Ala Leu Leu Ser Cys Leu Thr Val Pro Ala Ser Ala 
            180                 185                 190     


<210>  2
<211>  192
<212>  PRT
<213>  Hepatitis C virus

<400>  2

Tyr Gln Val Arg Asn Ser Thr Gly Leu Tyr His Val Thr Asn Asp Cys 
1               5                   10                  15      


Pro Asn Ser Ser Ile Val Tyr Glu Ala Ala Asp Ala Ile Leu His Thr 
            20                  25                  30          


Pro Gly Cys Val Pro Cys Val Arg Glu Gly Asn Ala Ser Arg Cys Trp 
        35                  40                  45              


Val Ala Met Thr Pro Thr Val Ala Thr Arg Asp Gly Lys Leu Pro Ala 
    50                  55                  60                  


Thr Gln Leu Arg Arg His Ile Asp Leu Leu Val Gly Ser Ala Thr Leu 
65                  70                  75                  80  


Cys Ser Ala Leu Tyr Val Gly Asp Leu Cys Gly Ser Val Phe Leu Val 
                85                  90                  95      


Gly Gln Leu Phe Thr Phe Ser Pro Arg Arg His Trp Thr Thr Gln Gly 
            100                 105                 110         


Cys Asn Cys Ser Ile Tyr Pro Gly His Ile Thr Gly His Arg Met Ala 
        115                 120                 125             


Trp Asp Met Met Met Asn Trp Ser Pro Thr Thr Ala Leu Val Met Ala 
    130                 135                 140                 


Gln Leu Leu Arg Ile Pro Gln Ala Ile Leu Asp Met Ile Ala Gly Ala 
145                 150                 155                 160 


His Trp Gly Val Leu Ala Gly Ile Ala Tyr Phe Ser Met Val Gly Asn 
                165                 170                 175     


Trp Ala Lys Val Leu Val Val Leu Leu Leu Phe Ala Gly Val Asp Ala 
            180                 185                 190         


<210>  3
<211>  363
<212>  PRT
<213>  Hepatitis C virus

<400>  3

Glu Thr His Val Thr Gly Gly Ser Ala Gly His Thr Val Ser Gly Phe 
1               5                   10                  15      


Val Ser Leu Leu Ala Pro Gly Ala Lys Gln Asn Val Gln Leu Ile Asn 
            20                  25                  30          


Thr Asn Gly Ser Trp His Leu Asn Ser Thr Ala Leu Asn Cys Asn Asp 
        35                  40                  45              


Ser Leu Asn Thr Gly Trp Leu Ala Gly Leu Phe Tyr His His Lys Phe 
    50                  55                  60                  


Asn Ser Ser Gly Cys Pro Glu Arg Leu Ala Ser Cys Arg Pro Leu Thr 
65                  70                  75                  80  


Asp Phe Asp Gln Gly Trp Gly Pro Ile Ser Tyr Ala Asn Gly Ser Gly 
                85                  90                  95      


Pro Asp Gln Arg Pro Tyr Cys Trp His Tyr Pro Pro Lys Pro Cys Gly 
            100                 105                 110         


Ile Val Pro Ala Lys Ser Val Cys Gly Pro Val Tyr Cys Phe Thr Pro 
        115                 120                 125             


Ser Pro Val Val Val Gly Thr Thr Asp Arg Ser Gly Ala Pro Thr Tyr 
    130                 135                 140                 


Ser Trp Gly Glu Asn Asp Thr Asp Val Phe Val Leu Asn Asn Thr Arg 
145                 150                 155                 160 


Pro Pro Leu Gly Asn Trp Phe Gly Cys Thr Trp Met Asn Ser Thr Gly 
                165                 170                 175     


Phe Thr Lys Val Cys Gly Ala Pro Pro Cys Val Ile Gly Gly Ala Gly 
            180                 185                 190         


Asn Asn Thr Leu His Cys Pro Thr Asp Cys Phe Arg Lys His Pro Asp 
        195                 200                 205             


Ala Thr Tyr Ser Arg Cys Gly Ser Gly Pro Trp Ile Thr Pro Arg Cys 
    210                 215                 220                 


Leu Val Asp Tyr Pro Tyr Arg Leu Trp His Tyr Pro Cys Thr Ile Asn 
225                 230                 235                 240 


Tyr Thr Ile Phe Lys Ile Arg Met Tyr Val Gly Gly Val Glu His Arg 
                245                 250                 255     


Leu Glu Ala Ala Cys Asn Trp Thr Arg Gly Glu Arg Cys Asp Leu Glu 
            260                 265                 270         


Asp Arg Asp Arg Ser Glu Leu Ser Pro Leu Leu Leu Thr Thr Thr Gln 
        275                 280                 285             


Trp Gln Val Leu Pro Cys Ser Phe Thr Thr Leu Pro Ala Leu Ser Thr 
    290                 295                 300                 


Gly Leu Ile His Leu His Gln Asn Ile Val Asp Val Gln Tyr Leu Tyr 
305                 310                 315                 320 


Gly Val Gly Ser Ser Ile Ala Ser Trp Ala Ile Lys Trp Glu Tyr Val 
                325                 330                 335     


Val Leu Leu Phe Leu Leu Leu Ala Asp Ala Arg Val Cys Ser Cys Leu 
            340                 345                 350         


Trp Met Met Leu Leu Ile Ser Gln Ala Glu Ala 
        355                 360             


<210>  4
<211>  432
<212>  PRT
<213>  Artificial

<220>
<223>  HCV core 3-epitopes calibrator

<400>  4

Met Arg Gly Ser Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Arg Pro 
1               5                   10                  15      


Glu Gly Arg Thr Gly Gly Gly Ser Gly Gly Gly Met Lys Val Ala Lys 
            20                  25                  30          


Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg Thr Glu Asp Gly Val 
        35                  40                  45              


Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu Asp Tyr Leu His Gly 
    50                  55                  60                  


His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala Leu Glu Gly His Glu 
65                  70                  75                  80  


Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala Asn Asp Ala Tyr Gly 
                85                  90                  95      


Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro Lys Asp Val Phe Met 
            100                 105                 110         


Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe Leu Ala Glu Thr Asp 
        115                 120                 125             


Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val Glu Asp Asp His Val 
    130                 135                 140                 


Val Val Asp Gly Asn His Met Leu Ala Gly Gln Asn Leu Lys Phe Asn 
145                 150                 155                 160 


Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu Glu Glu Leu Ala His 
                165                 170                 175     


Gly His Val His Gly Ala His Asp His His His Asp His Asp His Asp 
            180                 185                 190         


Gly Gly Gly Ser Gly Gly Gly Leu Leu Ser Pro Arg Gly Ser Arg Pro 
        195                 200                 205             


Ser Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Gly Gly Gly Ser 
    210                 215                 220                 


Gly Gly Gly Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr 
225                 230                 235                 240 


Gln Val Arg Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser 
                245                 250                 255     


Ala Pro Leu Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu 
            260                 265                 270         


Glu Thr Ala Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala 
        275                 280                 285             


Val Gly Ala Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln 
    290                 295                 300                 


Arg Val Pro Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly 
305                 310                 315                 320 


Met Arg Phe Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile 
                325                 330                 335     


Thr Ala Val Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu 
            340                 345                 350         


Ala Gly Gln Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu 
        355                 360                 365             


Ala Thr Glu Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp 
    370                 375                 380                 


His His His Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ala 
385                 390                 395                 400 


His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 
                405                 410                 415     


Leu Pro Gly Gly Gly Gly Ser Gly Gly Gly His His His His His His 
            420                 425                 430         


<210>  5
<211>  473
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HCVcore-3-epitopes calibrator (HCVcore-3Epi-EcS-FP, XY(Z, 
       extralong, C/A))

<400>  5

Met Arg Gly Ser Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Arg Pro 
1               5                   10                  15      


Glu Gly Arg Thr Gly Gly Gly Ser Gly Gly Gly Met Lys Val Ala Lys 
            20                  25                  30          


Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg Thr Glu Asp Gly Val 
        35                  40                  45              


Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu Asp Tyr Leu His Gly 
    50                  55                  60                  


His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala Leu Glu Gly His Glu 
65                  70                  75                  80  


Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala Asn Asp Ala Tyr Gly 
                85                  90                  95      


Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro Lys Asp Val Phe Met 
            100                 105                 110         


Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe Leu Ala Glu Thr Asp 
        115                 120                 125             


Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val Glu Asp Asp His Val 
    130                 135                 140                 


Val Val Asp Gly Asn His Met Leu Ala Gly Gln Asn Leu Lys Phe Asn 
145                 150                 155                 160 


Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu Glu Glu Leu Ala His 
                165                 170                 175     


Gly His Val His Gly Ala His Asp His His His Asp His Asp His Asp 
            180                 185                 190         


Gly Gly Gly Ser Gly Gly Gly Leu Leu Ser Pro Arg Gly Ser Arg Pro 
        195                 200                 205             


Ser Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Gly Gly Gly Ser 
    210                 215                 220                 


Gly Gly Gly Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr 
225                 230                 235                 240 


Gln Val Arg Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser 
                245                 250                 255     


Ala Pro Leu Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu 
            260                 265                 270         


Glu Thr Ala Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala 
        275                 280                 285             


Val Gly Ala Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln 
    290                 295                 300                 


Arg Val Pro Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly 
305                 310                 315                 320 


Met Arg Phe Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile 
                325                 330                 335     


Thr Ala Val Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu 
            340                 345                 350         


Ala Gly Gln Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu 
        355                 360                 365             


Ala Thr Glu Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp 
    370                 375                 380                 


His His His Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Asp 
385                 390                 395                 400 


Pro Arg Arg Arg Ser Arg Asn Leu Gly Lys Val Ile Asp Thr Leu Thr 
                405                 410                 415     


Ala Gly Phe Ala Asp Leu Met Gly Tyr Ile Pro Leu Val Gly Ala Pro 
            420                 425                 430         


Leu Gly Gly Ala Ala Arg Ala Leu Ala His Gly Val Arg Val Leu Glu 
        435                 440                 445             


Asp Gly Val Asn Tyr Ala Thr Gly Asn Leu Pro Gly Gly Gly Gly Ser 
    450                 455                 460                 


Gly Gly Gly His His His His His His 
465                 470             


<210>  6
<211>  513
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HCVcore-3-epitopes calibrator (HCVcore-3Epi-EcS-FP, XY(Z)4)

<400>  6

Met Arg Gly Ser Gly Arg Arg Gln Pro Ile Pro Lys Ala Arg Arg Pro 
1               5                   10                  15      


Glu Gly Arg Thr Gly Gly Gly Ser Gly Gly Gly Met Lys Val Ala Lys 
            20                  25                  30          


Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg Thr Glu Asp Gly Val 
        35                  40                  45              


Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu Asp Tyr Leu His Gly 
    50                  55                  60                  


His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala Leu Glu Gly His Glu 
65                  70                  75                  80  


Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala Asn Asp Ala Tyr Gly 
                85                  90                  95      


Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro Lys Asp Val Phe Met 
            100                 105                 110         


Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe Leu Ala Glu Thr Asp 
        115                 120                 125             


Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val Glu Asp Asp His Val 
    130                 135                 140                 


Val Val Asp Gly Asn His Met Leu Ala Gly Gln Asn Leu Lys Phe Asn 
145                 150                 155                 160 


Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu Glu Glu Leu Ala His 
                165                 170                 175     


Gly His Val His Gly Ala His Asp His His His Asp His Asp His Asp 
            180                 185                 190         


Gly Gly Gly Ser Gly Gly Gly Leu Leu Ser Pro Arg Gly Ser Arg Pro 
        195                 200                 205             


Ser Trp Gly Pro Thr Asp Pro Arg Arg Arg Ser Arg Gly Gly Gly Ser 
    210                 215                 220                 


Gly Gly Gly Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr 
225                 230                 235                 240 


Gln Val Arg Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser 
                245                 250                 255     


Ala Pro Leu Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu 
            260                 265                 270         


Glu Thr Ala Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala 
        275                 280                 285             


Val Gly Ala Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln 
    290                 295                 300                 


Arg Val Pro Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly 
305                 310                 315                 320 


Met Arg Phe Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile 
                325                 330                 335     


Thr Ala Val Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu 
            340                 345                 350         


Ala Gly Gln Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu 
        355                 360                 365             


Ala Thr Glu Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp 
    370                 375                 380                 


His His His Asp His Asp His Asp Gly Gly Gly Ser Gly Gly Gly Ala 
385                 390                 395                 400 


His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn 
                405                 410                 415     


Leu Pro Gly Gly Gly Gly Ser Gly Gly Gly Ala His Gly Val Arg Val 
            420                 425                 430         


Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly Asn Leu Pro Gly Gly Gly 
        435                 440                 445             


Gly Ser Gly Gly Gly Ala His Gly Val Arg Val Leu Glu Asp Gly Val 
    450                 455                 460                 


Asn Tyr Ala Thr Gly Asn Leu Pro Gly Gly Gly Gly Ser Gly Gly Gly 
465                 470                 475                 480 


Ala His Gly Val Arg Val Leu Glu Asp Gly Val Asn Tyr Ala Thr Gly 
                485                 490                 495     


Asn Leu Pro Gly Gly Gly Gly Ser Gly Gly Gly His His His His His 
            500                 505                 510         


His 
    


