                         SEQUENCE LISTING

<110>  Montana Molecular, LLC
 
<120>  BIOSENSORS FOR MEASURING CELL SIGNALING IN STRESSED

<130>  602889 081638-003PC

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XBP1 intron

<400>  1
cagcactcag actacgtgca cctctg                                            26


<210>  2
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XBP1 intron structure sequence

<400>  2
ccgggtctgc tgagtccgca gcactcagac tacgtgcacc tctgcagcag gtgcaggccc       60

ag                                                                      62


<210>  3
<211>  201
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  XBP1 partial coding sequence, including XBP1 intron

<400>  3
actcatggcc ttgtagttga gaaccaggag ttaagacagc gcttggggat ggatgccctg       60

gttgctgaag aggaggcgga agccaagggg aatgaagtga ggccagtggc cgggtctgct      120

gagtccgcag cactcagact acgtgcacct ctgcagcagg tgcaggccca gttgtcaccc      180

ctccagaaca tctccccatg g                                                201


<210>  4
<211>  2231
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RGECO-mNeonGreen calcium detection construct


<220>
<221>  misc_feature
<222>  (1)..(1251)
<223>  RGECO calcium sensor

<220>
<221>  misc_feature
<222>  (1252)..(1305)
<223>  T2A protein coding sequence

<220>
<221>  misc_feature
<222>  (1324)..(1524)
<223>  Partial sequence of XBP1 protein, including intron and intron 
       structure sequences

<220>
<221>  misc_feature
<222>  (1433)..(1494)
<223>  XBP1 intron structure sequence

<220>
<221>  misc_feature
<222>  (1451)..(1476)
<223>  XBP1 intron sequence

<220>
<221>  misc_feature
<222>  (1521)..(2231)
<223>  mNeonGreen

<220>
<221>  misc_feature
<222>  (1525)..(1527)
<223>  Stop codon before intron is spliced out

<400>  4
atggtcgact catcacgtcg taagtggaat aaggcaggtc acgcagtcag agctataggt       60

cggctgagct cacccgtggt ttccgagcgg atgtaccccg aggacggcgc cctgaagagc      120

gagatcaaga aggggctgag gctgaaggac ggcggccact acgccgccga ggtcaagacc      180

acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg      240

gacatcgtgt cccacaacga ggactacacc atcgtggaac agtgcgaacg cgccgagggc      300

cgccactcca ccggcggcat ggacgagctg tacaagggag gtacaggcgg gagtctggtg      360

agcaagggcg aggaggataa cagggccatc gtcaaggagt tcatgcgctt caagttgcac      420

atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc      480

tacgaggcct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgca      540

tgggacatcc tgtcccctca gttgatgtac ggctccaagg cctacattaa gcacccagcc      600

gacatccccg actacttcaa gctgtccttc cccgagggct tcaggtggga gcgcgtgatg      660

aacttcgagg acggcggcat tattcacgtt agccaggaca cctccctgca ggacggcgta      720

ttcatctaca aggtgaagct gcgcggcacc aacttccccc ccgacggccc cgtaatgcag      780

aagaagacca tgggctggga ggctacgcgt gaccaactga ctgaagagca gatcgcagaa      840

tttaaagagg ctttctccct atttgacaag gacggggatg ggacgatgac aaccaaggag      900

ctggggacgg tgatgcggtc tctggggcag aaccccacag aagcagagct gcaggacatg      960

atcaatgaag tagatgccga cggtgacggc acattcgact tccctgagtt cctgacgatg     1020

atggcaagaa aaatgaatga cacagacagt gaagaggaaa ttagagaagc gttccgcgtg     1080

tttgataagg acggcaatgg ctacatcggc gcagcagagc ttcgccacgt gatgacagac     1140

cttggagaga agataacaga tgaggaggtt gatgaaatga tcagggtagc agacatcgat     1200

ggggatggtc aggtaaacta tgaagagttt gtacaaatga tgacagcgaa ggagggcaga     1260

ggaagtcttc taacatgcgg tgacgtggag gagaatcccg ggcctctagc gtttaccatg     1320

gttactcatg gccttgtagt tgagaaccag gagttaagac agcgcttggg gatggatgcc     1380

ctggttgctg aagaggaggc ggaagccaag gggaatgaag tgaggccagt ggccgggtct     1440

gctgagtccg cagcactcag actacgtgca cctctgcagc aggtgcaggc ccagttgtca     1500

cccctccaga acatctcccc atggtgagca agggcgagga ggataacatg gcctctctcc     1560

cagcgacaca tgagttacac atctttggct ccatcaacgg tgtggacttt gacatggtgg     1620

gtcagggcac cggcaatcca aatgatggtt atgaggagtt aaacctgaag tccaccaagg     1680

gtgacctcca gttctccccc tggattctgg tccctcatat cgggtatggc ttccatcagt     1740

acctgcccta ccctgacggg atgtcgcctt tccaggccgc catggtagat ggctccggat     1800

accaagtcca tcgcacaatg cagtttgaag atggtgcctc ccttactgtt aactaccgct     1860

acacctacga gggaagccac atcaaaggag aggcccaggt gaaggggact ggtttccctg     1920

ctgacggtcc tgtgatgacc aactcgctga ccgctgcgga ctggtgcagg tcgaagaaga     1980

cttaccccaa cgacaaaacc atcatcagta cctttaagtg gagttacacc actggaaatg     2040

gcaagcgcta ccggagcact gcgcggacca cctacacctt tgccaagcca atggcggcta     2100

actatctgaa gaaccagccg atgtacgtgt tccgtaagac ggagctcaag cactccaaga     2160

ccgagctcaa cttcaaggag tggcaaaagg cctttaccga tgtgatgggc atggacgagc     2220

tgtacaagta a                                                          2231


<210>  5
<211>  797
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Single color cell stress biosensor construct


<220>
<221>  misc_feature
<222>  (1)..(273)
<223>  circularly permuted mNeonGreen exon

<220>
<221>  misc_feature
<222>  (274)..(335)
<223>  XBP1 intron structure sequence

<220>
<221>  Intron
<222>  (292)..(317)
<223>  XBP1 intron sequence

<220>
<221>  misc_feature
<222>  (336)..(797)
<223>  circularly permuted mNeonGreen exon

<400>  5
atggtttcga agaagactta ccccaacgac aaaaccatca tcagtacctt taagtggagt       60

tacaccactg gaaatggcaa gcgctaccgg agcactgcgc ggaccaccta cacctttgcc      120

aagccaatgg cggctaacta tctgaagaac cagccgatgt acgtgttccg taagacggag      180

ctcaagcact ccaagaccga gctcaacttc aaggagtggc aaaaggcctt taccgatgtg      240

atgggcatgg acgagctgta caagggcggt accccgggtc tgctgagtcc gcagcactca      300

gactacgtgc acctctgcag caggtgcagg cccagggagg gagcatggtg agcaagggcg      360

aggaggataa catggcctct ctcccagcga cacatgagtt acacatcttt ggctccatca      420

acggtgtgga ctttgacatg gtgggtcagg gcaccggcaa tccaaatgat ggttatgagg      480

agttaaacct gaagtccacc aagggtgacc tccagttctc cccctggatt ctggtccctc      540

atatcgggta tggcttccat cagtacctgc cctaccctga cgggatgtcg cctttccagg      600

ccgccatggt agatggctcc ggataccaag tccatcgcac aatgcagttt gaagatggtg      660

cctcccttac tgttaactac cgctacacct acgagggaag ccacatcaaa ggagaggccc      720

aggtgaaggg gactggtttc cctgctgacg gtcctgtgat gaccaactcg ctgaccgctg      780

cggactggtg caggtag                                                     797


<210>  6
<211>  1814
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Multiplex fluorescent stress sensor construct


<220>
<221>  misc_feature
<222>  (4)..(24)
<223>  SV40 nuclear localization signal

<220>
<221>  misc_feature
<222>  (25)..(813)
<223>  Red fluorescent protein

<220>
<221>  misc_feature
<222>  (814)..(867)
<223>  T2A sequence

<220>
<221>  misc_feature
<222>  (886)..(1086)
<223>  Partial sequence of XBP1 protein, including intron and intron 
       structure sequences

<220>
<221>  misc_feature
<222>  (995)..(1056)
<223>  XBP1 intron structure sequence

<220>
<221>  misc_feature
<222>  (1083)..(1790)
<223>  mNeonGreen

<220>
<221>  misc_feature
<222>  (1087)..(1089)
<223>  Stop codon before intron is spliced out

<220>
<221>  misc_feature
<222>  (1791)..(1811)
<223>  SV40 nuclear localization signal

<400>  6
atgcctaaga agaagaggaa ggtcatggtg tcgaagggag aagaactgat taaggaaaac       60

atgcggatga aagtggtgat ggaagggtcg gtgaatggac accaattcaa gtgcaccgga      120

gagggagagg gcaacccata catgggtact cagaccatgc gcatcaaggt cgttgaagga      180

ggacctctgc cttttgcgtt cgacatcctt gctacctcgt tcatgtacgg gtcccgcacc      240

tttatcaagt acccgaaggg aatcccggat ttcttcaagc agagcttccc ggaaggattc      300

acctgggaga gggtgactcg gtacgaagat ggaggagtgc tgactgcaac ccaagacact      360

tcgctcgagg acggctgtct ggtgtaccat gtccaagtgc ggggtgtgaa cttcccctca      420

aatgggccag tgatgcagaa aaagaccctc ggatgggaag cgaacaccga gatgatgtac      480

ccggcggacg gtggcttgcg aggatacact cacatggcct tgaagctgga cggcggaggt      540

catctctcat gctcctttgt cactacctac cgcagcaaga aaactgtcgg aaacatcaag      600

atgccgggcg tgtactacgt cgatcaccgg ctcgagagaa tcaaagaggc cgacaaggaa      660

acgtatgtcg agcagcatga agtcgcagtg gccaggtact gcgaccttcc ctccaaactg      720

ggccacaagc tgaattctgg cctgagaagc cgcgcacagg cttcgaactc agccgtggat      780

gggacggccg gcccagggtc cactggaagc agagagggca gaggaagtct tctaacatgc      840

ggtgacgtgg aggagaatcc cgggcctcta gcgtttacca tggttactca tggccttgta      900

gttgagaacc aggagttaag acagcgcttg gggatggatg ccctggttgc tgaagaggag      960

gcggaagcca aggggaatga agtgaggcca gtggccgggt ctgctgagtc cgcagcactc     1020

agactacgtg cacctctgca gcaggtgcag gcccagttgt cacccctcca gaacatctcc     1080

ccatggtgag caagggcgag gaggataaca tggcctctct cccagcgaca catgagttac     1140

acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc accggcaatc     1200

caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc cagttctccc     1260

cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc taccctgacg     1320

ggatgtcgcc tttccaggcc gccatggtag atggctccgg ataccaagtc catcgcacaa     1380

tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac gagggaagcc     1440

acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt cctgtgatga     1500

ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa gacttacccc aacgacaaaa     1560

ccatcatcag tacctttaag tggagttaca ccactggaaa tggcaagcgc taccggagca     1620

ctgcgcggac cacctacacc tttgccaagc caatggcggc taactatctg aagaaccagc     1680

cgatgtacgt gttccgtaag acggagctca agcactccaa gaccgagctc aacttcaagg     1740

agtggcaaaa ggcctttacc gatgtgatgg gcatggacga gctgtacaag cctaagaaga     1800

agaggaaggt ctaa                                                       1814


