                         SEQUENCE LISTING

<110>  The BROAD Institute, Inc.
       The Massachusetts Institute of Technology
       The New York Genome Center
       The President and Fellows of Harvard College
       Sanjana, Neville
       Zhang, Feng
       Meier, Joshua A.
 
<120>  Methods for Designing Guide Sequences for Guided Nucleases

<130>  BROD-2145WP

<140>  PCT/US2018/041041
<141>  2018-07-06

<150>  US 62/529,573
<151>  2017-07-07

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  73
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (18)..(38)
<223>  n may represent any nucleotide or may reflect a placeholder for a
       nucleotide not present

<400>  1
ggaaaggacg aaacaccgnn nnnnnnnnnn nnnnnnnngt tttagagcta gaaatagcaa       60

gttaaaataa ggc                                                          73


<210>  2
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  2
taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg       60


<210>  3
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  3
actttttcaa gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac       60


<210>  4
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<222>  (18)..(39)
<223>  n may represent any amino acid or may reflect a placeholder for a
       nucleotide not present

<400>  4
ggaaaggacg aaacaccgnn nnnnnnnnnn nnnnnnnngt ttaagagcta tgctggaaac       60

agc                                                                     63


<210>  5
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  5
taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg       60


<210>  6
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  6
gactagcctt atttaaactt gctatgctgt ttccagcata gctcttaaac                  50


<210>  7
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  7
acttgtttaa gt                                                           12


<210>  8
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  8
ggcaccgagt cggtgc                                                       16


<210>  9
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  9

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  10
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  10

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25                  30  


<210>  11
<211>  45
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  11

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
        35                  40                  45  


<210>  12
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  12

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    50                  55                  60  


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  13
tcaccccaaa tatcatcagt                                                   20


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  14
gttgcaccct tccagacttg                                                   20


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  15
ggcagagaat gcttgtaccc                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  16
gatcttgcgt atgctggaga                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  17
gataggcttg atgtcctggg                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic

<400>  18
tactcatccg caagtctgga                                                   20


