               SEQUENCE LISTING

<110> Merz Pharma GmbH & Co. KGaA

<120> PAS-BoNT with increased activity

<130> 1917-000-PCT

<160> 5

<170> BiSSAP 1.3.6

<210> 1
<211> 1335
<212> PRT
<213> Artificial Sequence


<220> 
<223> SEQ NO 1

<400> 1
Met Pro Phe Val Asn Lys Gln Phe Asn Tyr Lys Asp Pro Val Asn Gly 
1               5                   10                  15      
Val Asp Ile Ala Tyr Ile Lys Ile Pro Asn Ala Gly Gln Met Gln Pro 
            20                  25                  30          
Val Lys Ala Phe Lys Ile His Asn Lys Ile Trp Val Ile Pro Glu Arg 
        35                  40                  45              
Asp Thr Phe Thr Asn Pro Glu Glu Gly Asp Leu Asn Pro Pro Pro Glu 
    50                  55                  60                  
Ala Lys Gln Val Pro Val Ser Tyr Tyr Asp Ser Thr Tyr Leu Ser Thr 
65                  70                  75                  80  
Asp Asn Glu Lys Asp Asn Tyr Leu Lys Gly Val Thr Lys Leu Phe Glu 
                85                  90                  95      
Arg Ile Tyr Ser Thr Asp Leu Gly Arg Met Leu Leu Thr Ser Ile Val 
            100                 105                 110         
Arg Gly Ile Pro Phe Trp Gly Gly Ser Thr Ile Asp Thr Glu Leu Lys 
        115                 120                 125             
Val Ile Asp Thr Asn Cys Ile Asn Val Ile Gln Pro Asp Gly Ser Tyr 
    130                 135                 140                 
Arg Ser Glu Glu Leu Asn Leu Val Ile Ile Gly Pro Ser Ala Asp Ile 
145                 150                 155                 160 
Ile Gln Phe Glu Cys Lys Ser Phe Gly His Glu Val Leu Asn Leu Thr 
                165                 170                 175     
Arg Asn Gly Tyr Gly Ser Thr Gln Tyr Ile Arg Phe Ser Pro Asp Phe 
            180                 185                 190         
Thr Phe Gly Phe Glu Glu Ser Leu Glu Val Asp Thr Asn Pro Leu Leu 
        195                 200                 205             
Gly Ala Gly Lys Phe Ala Thr Asp Pro Ala Val Thr Leu Ala His Glu 
    210                 215                 220                 
Leu Ile His Ala Gly His Arg Leu Tyr Gly Ile Ala Ile Asn Pro Asn 
225                 230                 235                 240 
Arg Val Phe Lys Val Asn Thr Asn Ala Tyr Tyr Glu Met Ser Gly Leu 
                245                 250                 255     
Glu Val Ser Phe Glu Glu Leu Arg Thr Phe Gly Gly His Asp Ala Lys 
            260                 265                 270         
Phe Ile Asp Ser Leu Gln Glu Asn Glu Phe Arg Leu Tyr Tyr Tyr Asn 
        275                 280                 285             
Lys Phe Lys Asp Ile Ala Ser Thr Leu Asn Lys Ala Lys Ser Ile Val 
    290                 295                 300                 
Gly Thr Thr Ala Ser Leu Gln Tyr Met Lys Asn Val Phe Lys Glu Lys 
305                 310                 315                 320 
Tyr Leu Leu Ser Glu Asp Thr Ser Gly Lys Phe Ser Val Asp Lys Leu 
                325                 330                 335     
Lys Phe Asp Lys Leu Tyr Lys Met Leu Thr Glu Ile Tyr Thr Glu Asp 
            340                 345                 350         
Asn Phe Val Lys Phe Phe Lys Val Leu Asn Arg Lys Thr Tyr Leu Asn 
        355                 360                 365             
Phe Asp Lys Ala Val Phe Lys Ile Asn Ile Val Pro Lys Val Asn Tyr 
    370                 375                 380                 
Thr Ile Tyr Asp Gly Phe Asn Leu Arg Asn Thr Asn Leu Ala Ala Asn 
385                 390                 395                 400 
Phe Asn Gly Gln Asn Thr Glu Ile Asn Asn Met Asn Phe Thr Lys Leu 
                405                 410                 415     
Lys Asn Phe Thr Gly Leu Phe Glu Phe Tyr Lys Leu Leu Cys Val Arg 
            420                 425                 430         
Gly Ile Ile Thr Ser Lys Ala Gly Ala Gly Lys Ser Leu Val Pro Arg 
        435                 440                 445             
Gly Ser Ala Gly Ala Gly Ala Leu Asn Asp Leu Cys Ile Lys Val Asn 
    450                 455                 460                 
Asn Trp Asp Leu Phe Phe Ser Pro Ser Glu Asp Asn Phe Thr Asn Asp 
465                 470                 475                 480 
Leu Asn Lys Gly Glu Glu Ile Thr Ser Asp Thr Asn Ile Glu Ala Ala 
                485                 490                 495     
Glu Glu Asn Ile Ser Leu Asp Leu Ile Gln Gln Tyr Tyr Leu Thr Phe 
            500                 505                 510         
Asn Phe Asp Asn Glu Pro Glu Asn Ile Ser Ile Glu Asn Leu Ser Ser 
        515                 520                 525             
Asp Ile Ile Gly Gln Leu Glu Leu Met Pro Asn Ile Glu Arg Phe Pro 
    530                 535                 540                 
Asn Gly Lys Lys Tyr Glu Leu Asp Lys Tyr Thr Met Phe His Tyr Leu 
545                 550                 555                 560 
Arg Ala Gln Glu Phe Glu His Gly Lys Ser Arg Ile Ala Leu Thr Asn 
                565                 570                 575     
Ser Val Asn Glu Ala Leu Leu Asn Pro Ser Arg Val Tyr Thr Phe Phe 
            580                 585                 590         
Ser Ser Asp Tyr Val Lys Lys Val Asn Lys Ala Thr Glu Ala Ala Met 
        595                 600                 605             
Phe Leu Gly Trp Val Glu Gln Leu Val Tyr Asp Phe Thr Asp Glu Thr 
    610                 615                 620                 
Ser Glu Val Ser Thr Thr Asp Lys Ile Ala Asp Ile Thr Ile Ile Ile 
625                 630                 635                 640 
Pro Tyr Ile Gly Pro Ala Leu Asn Ile Gly Asn Met Leu Tyr Lys Asp 
                645                 650                 655     
Asp Phe Val Gly Ala Leu Ile Phe Ser Gly Ala Val Ile Leu Leu Glu 
            660                 665                 670         
Phe Ile Pro Glu Ile Ala Ile Pro Val Leu Gly Thr Phe Ala Leu Val 
        675                 680                 685             
Ser Tyr Ile Ala Asn Lys Val Leu Thr Val Gln Thr Ile Asp Asn Ala 
    690                 695                 700                 
Leu Ser Lys Arg Asn Glu Lys Trp Asp Glu Val Tyr Lys Tyr Ile Val 
705                 710                 715                 720 
Thr Asn Trp Leu Ala Lys Val Asn Thr Gln Ile Asp Leu Ile Arg Lys 
                725                 730                 735     
Lys Met Lys Glu Ala Leu Glu Asn Gln Ala Glu Ala Thr Lys Ala Ile 
            740                 745                 750         
Ile Asn Tyr Gln Tyr Asn Gln Tyr Thr Glu Glu Glu Lys Asn Asn Ile 
        755                 760                 765             
Asn Phe Asn Ile Asp Asp Leu Ser Ser Lys Leu Asn Glu Ser Ile Asn 
    770                 775                 780                 
Lys Ala Met Ile Asn Ile Asn Lys Phe Leu Asn Gln Cys Ser Val Ser 
785                 790                 795                 800 
Tyr Leu Met Asn Ser Met Ile Pro Tyr Gly Val Lys Arg Leu Glu Asp 
                805                 810                 815     
Phe Asp Ala Ser Leu Lys Asp Ala Leu Leu Lys Tyr Ile Tyr Asp Asn 
            820                 825                 830         
Arg Gly Thr Leu Ile Gly Gln Val Asp Arg Leu Lys Asp Lys Val Asn 
        835                 840                 845             
Asn Thr Leu Ser Thr Asp Ile Pro Phe Gln Leu Ser Lys Tyr Val Asp 
    850                 855                 860                 
Asn Gln Arg Leu Leu Ser Thr Phe Thr Glu Tyr Ile Lys Asn Ile Ile 
865                 870                 875                 880 
Asn Thr Ser Ile Leu Asn Leu Arg Tyr Glu Ser Asn His Leu Ile Asp 
                885                 890                 895     
Leu Ser Arg Tyr Ala Ser Lys Ile Asn Ile Gly Ser Lys Val Asn Phe 
            900                 905                 910         
Asp Pro Ile Asp Lys Asn Gln Ile Gln Leu Phe Asn Leu Glu Ser Ser 
        915                 920                 925             
Lys Ile Glu Val Ile Leu Lys Asn Ala Ile Val Tyr Asn Ser Met Tyr 
    930                 935                 940                 
Glu Asn Phe Ser Thr Ser Phe Trp Ile Arg Ile Pro Lys Tyr Phe Asn 
945                 950                 955                 960 
Ser Ile Ser Leu Asn Asn Glu Tyr Thr Ile Ile Asn Cys Met Glu Asn 
                965                 970                 975     
Asn Ser Gly Trp Lys Val Ser Leu Asn Tyr Gly Glu Ile Ile Trp Thr 
            980                 985                 990         
Leu Gln Asp Thr Gln Glu Ile Lys Gln Arg Val Val Phe Lys Tyr Ser 
        995                 1000                1005            
Gln Met Ile Asn Ile Ser Asp Tyr Ile Asn Arg Trp Ile Phe Val Thr 
    1010                1015                1020                
Ile Thr Asn Asn Arg Leu Asn Asn Ser Lys Ile Tyr Ile Asn Gly Arg 
1025                1030                1035                1040
Leu Ile Asp Gln Lys Pro Ile Ser Asn Leu Gly Asn Ile His Ala Ser 
                1045                1050                1055    
Asn Asn Ile Met Phe Lys Leu Asp Gly Cys Arg Asp Thr His Arg Tyr 
            1060                1065                1070        
Ile Trp Ile Lys Tyr Phe Asn Leu Phe Asp Lys Glu Leu Asn Glu Lys 
        1075                1080                1085            
Glu Ile Lys Asp Leu Tyr Asp Asn Gln Ser Asn Ser Gly Ile Leu Lys 
    1090                1095                1100                
Asp Phe Trp Gly Asp Tyr Leu Gln Tyr Asp Lys Pro Tyr Tyr Met Leu 
1105                1110                1115                1120
Asn Leu Tyr Asp Pro Asn Lys Tyr Val Asp Val Asn Asn Val Gly Ile 
                1125                1130                1135    
Arg Gly Tyr Met Tyr Leu Lys Gly Pro Arg Gly Ser Val Met Thr Thr 
            1140                1145                1150        
Asn Ile Tyr Leu Asn Ser Ser Leu Tyr Arg Gly Thr Lys Phe Ile Ile 
        1155                1160                1165            
Lys Lys Tyr Ala Ser Gly Asn Lys Asp Asn Ile Val Arg Asn Asn Asp 
    1170                1175                1180                
Arg Val Tyr Ile Asn Val Val Val Lys Asn Lys Glu Tyr Arg Leu Ala 
1185                1190                1195                1200
Thr Asn Ala Ser Gln Ala Gly Val Glu Lys Ile Leu Ser Ala Leu Glu 
                1205                1210                1215    
Ile Pro Asp Val Gly Asn Leu Ser Gln Val Val Val Met Lys Ser Lys 
            1220                1225                1230        
Asn Asp Gln Gly Ile Thr Asn Lys Cys Lys Met Asn Leu Gln Asp Asn 
        1235                1240                1245            
Asn Gly Asn Asp Ile Gly Phe Ile Gly Phe His Gln Phe Asn Asn Ile 
    1250                1255                1260                
Ala Lys Leu Val Ala Ser Asn Trp Tyr Asn Arg Gln Ile Glu Arg Ser 
1265                1270                1275                1280
Ser Arg Thr Leu Gly Cys Ser Trp Glu Phe Ile Pro Val Asp Asp Gly 
                1285                1290                1295    
Trp Gly Glu Arg Pro Leu Gly Asp Leu Val Pro Arg Gly Ser Ala Asn 
            1300                1305                1310        
Ser Ser Ser Val Asp Lys Leu Trp Ser His Pro Gln Phe Glu Lys Leu 
        1315                1320                1325            
Glu His His His His His His 
    1330                1335

<210> 2
<211> 1546
<212> PRT
<213> Artificial Sequence


<220> 
<223> PAS100-BoNT/A (D102F T109R K340M I348L N353M K356R) -PAS100
      including His.tag

<400> 2
Met Gly Ser Ser His His His His His His Gly Ser Leu Val Pro Arg 
1               5                   10                  15      
Ser Ser Ser Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala 
            20                  25                  30          
Pro Ala Pro Ser Ala Pro Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala 
        35                  40                  45              
Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala Ser Pro Ala Ala 
    50                  55                  60                  
Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala 
65                  70                  75                  80  
Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser 
                85                  90                  95      
Ala Pro Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala 
            100                 105                 110         
Pro Ala Pro Ser Ala Pro Ala Ala Pro Phe Val Asn Lys Gln Phe Asn 
        115                 120                 125             
Tyr Lys Asp Pro Val Asn Gly Val Asp Ile Ala Tyr Ile Lys Ile Pro 
    130                 135                 140                 
Asn Ala Gly Gln Met Gln Pro Val Lys Ala Phe Lys Ile His Asn Lys 
145                 150                 155                 160 
Ile Trp Val Ile Pro Glu Arg Asp Thr Phe Thr Asn Pro Glu Glu Gly 
                165                 170                 175     
Asp Leu Asn Pro Pro Pro Glu Ala Lys Gln Val Pro Val Ser Tyr Tyr 
            180                 185                 190         
Asp Ser Thr Tyr Leu Ser Thr Asp Asn Glu Lys Asp Asn Tyr Leu Lys 
        195                 200                 205             
Gly Val Thr Lys Leu Phe Glu Arg Ile Tyr Ser Thr Phe Leu Gly Arg 
    210                 215                 220                 
Met Leu Leu Arg Ser Ile Val Arg Gly Ile Pro Phe Trp Gly Gly Ser 
225                 230                 235                 240 
Thr Ile Asp Thr Glu Leu Lys Val Ile Asp Thr Asn Cys Ile Asn Val 
                245                 250                 255     
Ile Gln Pro Asp Gly Ser Tyr Arg Ser Glu Glu Leu Asn Leu Val Ile 
            260                 265                 270         
Ile Gly Pro Ser Ala Asp Ile Ile Gln Phe Glu Cys Lys Ser Phe Gly 
        275                 280                 285             
His Glu Val Leu Asn Leu Thr Arg Asn Gly Tyr Gly Ser Thr Gln Tyr 
    290                 295                 300                 
Ile Arg Phe Ser Pro Asp Phe Thr Phe Gly Phe Glu Glu Ser Leu Glu 
305                 310                 315                 320 
Val Asp Thr Asn Pro Leu Leu Gly Ala Gly Lys Phe Ala Thr Asp Pro 
                325                 330                 335     
Ala Val Thr Leu Ala His Glu Leu Ile His Ala Gly His Arg Leu Tyr 
            340                 345                 350         
Gly Ile Ala Ile Asn Pro Asn Arg Val Phe Lys Val Asn Thr Asn Ala 
        355                 360                 365             
Tyr Tyr Glu Met Ser Gly Leu Glu Val Ser Phe Glu Glu Leu Arg Thr 
    370                 375                 380                 
Phe Gly Gly His Asp Ala Lys Phe Ile Asp Ser Leu Gln Glu Asn Glu 
385                 390                 395                 400 
Phe Arg Leu Tyr Tyr Tyr Asn Lys Phe Lys Asp Ile Ala Ser Thr Leu 
                405                 410                 415     
Asn Lys Ala Lys Ser Ile Val Gly Thr Thr Ala Ser Leu Gln Tyr Met 
            420                 425                 430         
Lys Asn Val Phe Lys Glu Lys Tyr Leu Leu Ser Glu Asp Thr Ser Gly 
        435                 440                 445             
Lys Phe Ser Val Asp Lys Leu Lys Phe Asp Met Leu Tyr Lys Met Leu 
    450                 455                 460                 
Thr Glu Leu Tyr Thr Glu Asp Met Phe Val Arg Phe Phe Lys Val Leu 
465                 470                 475                 480 
Asn Arg Lys Thr Tyr Leu Asn Phe Asp Lys Ala Val Phe Lys Ile Asn 
                485                 490                 495     
Ile Val Pro Lys Val Asn Tyr Thr Ile Tyr Asp Gly Phe Asn Leu Arg 
            500                 505                 510         
Asn Thr Asn Leu Ala Ala Asn Phe Asn Gly Gln Asn Thr Glu Ile Asn 
        515                 520                 525             
Asn Met Asn Phe Thr Lys Leu Lys Asn Phe Thr Gly Leu Phe Glu Phe 
    530                 535                 540                 
Tyr Lys Leu Leu Cys Val Arg Gly Ile Ile Thr Ser Lys Ala Gly Ala 
545                 550                 555                 560 
Gly Lys Ser Leu Val Pro Arg Gly Ser Ala Gly Ala Gly Ala Leu Asn 
                565                 570                 575     
Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe Ser Pro Ser 
            580                 585                 590         
Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu Ile Thr Ser 
        595                 600                 605             
Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu Asp Leu Ile 
    610                 615                 620                 
Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro Glu Asn Ile 
625                 630                 635                 640 
Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu Glu Leu Met 
                645                 650                 655     
Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu Leu Asp Lys 
            660                 665                 670         
Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu His Gly Lys 
        675                 680                 685             
Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu Leu Asn Pro 
    690                 695                 700                 
Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys Lys Val Asn 
705                 710                 715                 720 
Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu Gln Leu Val 
                725                 730                 735     
Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr Asp Lys Ile 
            740                 745                 750         
Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile 
        755                 760                 765             
Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu Ile Phe Ser 
    770                 775                 780                 
Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala Ile Pro Val 
785                 790                 795                 800 
Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys Val Leu Thr 
                805                 810                 815     
Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu Lys Trp Asp 
            820                 825                 830         
Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys Val Asn Thr 
        835                 840                 845             
Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu Glu Asn Gln 
    850                 855                 860                 
Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn Gln Tyr Thr 
865                 870                 875                 880 
Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp Leu Ser Ser 
                885                 890                 895     
Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile Asn Lys Phe 
            900                 905                 910         
Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met Ile Pro Tyr 
        915                 920                 925             
Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys Asp Ala Leu 
    930                 935                 940                 
Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly Gln Val Asp 
945                 950                 955                 960 
Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp Ile Pro Phe 
                965                 970                 975     
Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser Thr Phe Thr 
            980                 985                 990         
Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr 
        995                 1000                1005            
Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn 
    1010                1015                1020                
Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln 
1025                1030                1035                1040
Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala 
                1045                1050                1055    
Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile 
            1060                1065                1070        
Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr 
        1075                1080                1085            
Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn 
    1090                1095                1100                
Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu Ile Lys Gln 
1105                1110                1115                1120
Arg Val Val Phe Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile 
                1125                1130                1135    
Asn Arg Trp Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser 
            1140                1145                1150        
Lys Ile Tyr Ile Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn 
        1155                1160                1165            
Leu Gly Asn Ile His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly 
    1170                1175                1180                
Cys Arg Asp Thr His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe 
1185                1190                1195                1200
Asp Lys Glu Leu Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln 
                1205                1210                1215    
Ser Asn Ser Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr 
            1220                1225                1230        
Asp Lys Pro Tyr Tyr Met Leu Asn Leu Tyr Asp Pro Asn Lys Tyr Val 
        1235                1240                1245            
Asp Val Asn Asn Val Gly Ile Arg Gly Tyr Met Tyr Leu Lys Gly Pro 
    1250                1255                1260                
Arg Gly Ser Val Met Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr 
1265                1270                1275                1280
Arg Gly Thr Lys Phe Ile Ile Lys Lys Tyr Ala Ser Gly Asn Lys Asp 
                1285                1290                1295    
Asn Ile Val Arg Asn Asn Asp Arg Val Tyr Ile Asn Val Val Val Lys 
            1300                1305                1310        
Asn Lys Glu Tyr Arg Leu Ala Thr Asn Ala Ser Gln Ala Gly Val Glu 
        1315                1320                1325            
Lys Ile Leu Ser Ala Leu Glu Ile Pro Asp Val Gly Asn Leu Ser Gln 
    1330                1335                1340                
Val Val Val Met Lys Ser Lys Asn Asp Gln Gly Ile Thr Asn Lys Cys 
1345                1350                1355                1360
Lys Met Asn Leu Gln Asp Asn Asn Gly Asn Asp Ile Gly Phe Ile Gly 
                1365                1370                1375    
Phe His Gln Phe Asn Asn Ile Ala Lys Leu Val Ala Ser Asn Trp Tyr 
            1380                1385                1390        
Asn Arg Gln Ile Glu Arg Ser Ser Arg Thr Leu Gly Cys Ser Trp Glu 
        1395                1400                1405            
Phe Ile Pro Val Asp Asp Gly Trp Gly Glu Arg Pro Leu Ala Ser Pro 
    1410                1415                1420                
Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro 
1425                1430                1435                1440
Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala 
                1445                1450                1455    
Pro Ser Ala Pro Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro 
            1460                1465                1470        
Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala Ser Pro Ala Ala Pro Ala 
        1475                1480                1485            
Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala Ser Pro 
    1490                1495                1500                
Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro 
1505                1510                1515                1520
Ala Gly Asp Leu Val Pro Arg Gly Ser Ala Asn Ser Ser Ser Val Asp 
                1525                1530                1535    
Lys Leu Trp Ser His Pro Gln Phe Glu Lys 
            1540                1545    

<210> 3
<211> 1546
<212> PRT
<213> Artificial Sequence


<220> 
<223> PAS100-BoNT/A (G169I, T220R, P239M, S254) -PAS100 including
      His.tag

<400> 3
Met Gly Ser Ser His His His His His His Gly Ser Leu Val Pro Arg 
1               5                   10                  15      
Ser Ser Ser Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala 
            20                  25                  30          
Pro Ala Pro Ser Ala Pro Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala 
        35                  40                  45              
Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala Ser Pro Ala Ala 
    50                  55                  60                  
Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala 
65                  70                  75                  80  
Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser 
                85                  90                  95      
Ala Pro Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala 
            100                 105                 110         
Pro Ala Pro Ser Ala Pro Ala Ala Pro Phe Val Asn Lys Gln Phe Asn 
        115                 120                 125             
Tyr Lys Asp Pro Val Asn Gly Val Asp Ile Ala Tyr Ile Lys Ile Pro 
    130                 135                 140                 
Asn Ala Gly Gln Met Gln Pro Val Lys Ala Phe Lys Ile His Asn Lys 
145                 150                 155                 160 
Ile Trp Val Ile Pro Glu Arg Asp Thr Phe Thr Asn Pro Glu Glu Gly 
                165                 170                 175     
Asp Leu Asn Pro Pro Pro Glu Ala Lys Gln Val Pro Val Ser Tyr Tyr 
            180                 185                 190         
Asp Ser Thr Tyr Leu Ser Thr Asp Asn Glu Lys Asp Asn Tyr Leu Lys 
        195                 200                 205             
Gly Val Thr Lys Leu Phe Glu Arg Ile Tyr Ser Thr Asp Leu Gly Arg 
    210                 215                 220                 
Met Leu Leu Thr Ser Ile Val Arg Gly Ile Pro Phe Trp Gly Gly Ser 
225                 230                 235                 240 
Thr Ile Asp Thr Glu Leu Lys Val Ile Asp Thr Asn Cys Ile Asn Val 
                245                 250                 255     
Ile Gln Pro Asp Gly Ser Tyr Arg Ser Glu Glu Leu Asn Leu Val Ile 
            260                 265                 270         
Ile Gly Pro Ser Ala Asp Ile Ile Gln Phe Glu Cys Lys Ser Phe Ile 
        275                 280                 285             
His Glu Val Leu Asn Leu Thr Arg Asn Gly Tyr Gly Ser Thr Gln Tyr 
    290                 295                 300                 
Ile Arg Phe Ser Pro Asp Phe Thr Phe Gly Phe Glu Glu Ser Leu Glu 
305                 310                 315                 320 
Val Asp Thr Asn Pro Leu Leu Gly Ala Gly Lys Phe Ala Thr Asp Pro 
                325                 330                 335     
Ala Val Arg Leu Ala His Glu Leu Ile His Ala Gly His Arg Leu Tyr 
            340                 345                 350         
Gly Ile Ala Ile Asn Met Asn Arg Val Phe Lys Val Asn Thr Asn Ala 
        355                 360                 365             
Tyr Tyr Glu Met Thr Gly Leu Glu Val Ser Phe Glu Glu Leu Arg Thr 
    370                 375                 380                 
Phe Gly Gly His Asp Ala Lys Phe Ile Asp Ser Leu Gln Glu Asn Glu 
385                 390                 395                 400 
Phe Arg Leu Tyr Tyr Tyr Asn Lys Phe Lys Asp Ile Ala Ser Thr Leu 
                405                 410                 415     
Asn Lys Ala Lys Ser Ile Val Gly Thr Thr Ala Ser Leu Gln Tyr Met 
            420                 425                 430         
Lys Asn Val Phe Lys Glu Lys Tyr Leu Leu Ser Glu Asp Thr Ser Gly 
        435                 440                 445             
Lys Phe Ser Val Asp Lys Leu Lys Phe Asp Lys Leu Tyr Lys Met Leu 
    450                 455                 460                 
Thr Glu Ile Tyr Thr Glu Asp Asn Phe Val Lys Phe Phe Lys Val Leu 
465                 470                 475                 480 
Asn Arg Lys Thr Tyr Leu Asn Phe Asp Lys Ala Val Phe Lys Ile Asn 
                485                 490                 495     
Ile Val Pro Lys Val Asn Tyr Thr Ile Tyr Asp Gly Phe Asn Leu Ser 
            500                 505                 510         
Asn Thr Asn Leu Ala Ala Asn Phe Asn Gly Gln Asn Thr Glu Ile Asn 
        515                 520                 525             
Asn Met Asn Phe Thr Lys Leu Lys Asn Phe Thr Gly Leu Phe Glu Phe 
    530                 535                 540                 
Tyr Lys Leu Leu Cys Val Arg Gly Ile Ile Thr Ser Lys Ala Gly Ala 
545                 550                 555                 560 
Gly Lys Ser Leu Val Pro Arg Gly Ser Ala Gly Ala Gly Ala Leu Asn 
                565                 570                 575     
Asp Leu Cys Ile Lys Val Asn Asn Trp Asp Leu Phe Phe Ser Pro Ser 
            580                 585                 590         
Glu Asp Asn Phe Thr Asn Asp Leu Asn Lys Gly Glu Glu Ile Thr Ser 
        595                 600                 605             
Asp Thr Asn Ile Glu Ala Ala Glu Glu Asn Ile Ser Leu Asp Leu Ile 
    610                 615                 620                 
Gln Gln Tyr Tyr Leu Thr Phe Asn Phe Asp Asn Glu Pro Glu Asn Ile 
625                 630                 635                 640 
Ser Ile Glu Asn Leu Ser Ser Asp Ile Ile Gly Gln Leu Glu Leu Met 
                645                 650                 655     
Pro Asn Ile Glu Arg Phe Pro Asn Gly Lys Lys Tyr Glu Leu Asp Lys 
            660                 665                 670         
Tyr Thr Met Phe His Tyr Leu Arg Ala Gln Glu Phe Glu His Gly Lys 
        675                 680                 685             
Ser Arg Ile Ala Leu Thr Asn Ser Val Asn Glu Ala Leu Leu Asn Pro 
    690                 695                 700                 
Ser Arg Val Tyr Thr Phe Phe Ser Ser Asp Tyr Val Lys Lys Val Asn 
705                 710                 715                 720 
Lys Ala Thr Glu Ala Ala Met Phe Leu Gly Trp Val Glu Gln Leu Val 
                725                 730                 735     
Tyr Asp Phe Thr Asp Glu Thr Ser Glu Val Ser Thr Thr Asp Lys Ile 
            740                 745                 750         
Ala Asp Ile Thr Ile Ile Ile Pro Tyr Ile Gly Pro Ala Leu Asn Ile 
        755                 760                 765             
Gly Asn Met Leu Tyr Lys Asp Asp Phe Val Gly Ala Leu Ile Phe Ser 
    770                 775                 780                 
Gly Ala Val Ile Leu Leu Glu Phe Ile Pro Glu Ile Ala Ile Pro Val 
785                 790                 795                 800 
Leu Gly Thr Phe Ala Leu Val Ser Tyr Ile Ala Asn Lys Val Leu Thr 
                805                 810                 815     
Val Gln Thr Ile Asp Asn Ala Leu Ser Lys Arg Asn Glu Lys Trp Asp 
            820                 825                 830         
Glu Val Tyr Lys Tyr Ile Val Thr Asn Trp Leu Ala Lys Val Asn Thr 
        835                 840                 845             
Gln Ile Asp Leu Ile Arg Lys Lys Met Lys Glu Ala Leu Glu Asn Gln 
    850                 855                 860                 
Ala Glu Ala Thr Lys Ala Ile Ile Asn Tyr Gln Tyr Asn Gln Tyr Thr 
865                 870                 875                 880 
Glu Glu Glu Lys Asn Asn Ile Asn Phe Asn Ile Asp Asp Leu Ser Ser 
                885                 890                 895     
Lys Leu Asn Glu Ser Ile Asn Lys Ala Met Ile Asn Ile Asn Lys Phe 
            900                 905                 910         
Leu Asn Gln Cys Ser Val Ser Tyr Leu Met Asn Ser Met Ile Pro Tyr 
        915                 920                 925             
Gly Val Lys Arg Leu Glu Asp Phe Asp Ala Ser Leu Lys Asp Ala Leu 
    930                 935                 940                 
Leu Lys Tyr Ile Tyr Asp Asn Arg Gly Thr Leu Ile Gly Gln Val Asp 
945                 950                 955                 960 
Arg Leu Lys Asp Lys Val Asn Asn Thr Leu Ser Thr Asp Ile Pro Phe 
                965                 970                 975     
Gln Leu Ser Lys Tyr Val Asp Asn Gln Arg Leu Leu Ser Thr Phe Thr 
            980                 985                 990         
Glu Tyr Ile Lys Asn Ile Ile Asn Thr Ser Ile Leu Asn Leu Arg Tyr 
        995                 1000                1005            
Glu Ser Asn His Leu Ile Asp Leu Ser Arg Tyr Ala Ser Lys Ile Asn 
    1010                1015                1020                
Ile Gly Ser Lys Val Asn Phe Asp Pro Ile Asp Lys Asn Gln Ile Gln 
1025                1030                1035                1040
Leu Phe Asn Leu Glu Ser Ser Lys Ile Glu Val Ile Leu Lys Asn Ala 
                1045                1050                1055    
Ile Val Tyr Asn Ser Met Tyr Glu Asn Phe Ser Thr Ser Phe Trp Ile 
            1060                1065                1070        
Arg Ile Pro Lys Tyr Phe Asn Ser Ile Ser Leu Asn Asn Glu Tyr Thr 
        1075                1080                1085            
Ile Ile Asn Cys Met Glu Asn Asn Ser Gly Trp Lys Val Ser Leu Asn 
    1090                1095                1100                
Tyr Gly Glu Ile Ile Trp Thr Leu Gln Asp Thr Gln Glu Ile Lys Gln 
1105                1110                1115                1120
Arg Val Val Phe Lys Tyr Ser Gln Met Ile Asn Ile Ser Asp Tyr Ile 
                1125                1130                1135    
Asn Arg Trp Ile Phe Val Thr Ile Thr Asn Asn Arg Leu Asn Asn Ser 
            1140                1145                1150        
Lys Ile Tyr Ile Asn Gly Arg Leu Ile Asp Gln Lys Pro Ile Ser Asn 
        1155                1160                1165            
Leu Gly Asn Ile His Ala Ser Asn Asn Ile Met Phe Lys Leu Asp Gly 
    1170                1175                1180                
Cys Arg Asp Thr His Arg Tyr Ile Trp Ile Lys Tyr Phe Asn Leu Phe 
1185                1190                1195                1200
Asp Lys Glu Leu Asn Glu Lys Glu Ile Lys Asp Leu Tyr Asp Asn Gln 
                1205                1210                1215    
Ser Asn Ser Gly Ile Leu Lys Asp Phe Trp Gly Asp Tyr Leu Gln Tyr 
            1220                1225                1230        
Asp Lys Pro Tyr Tyr Met Leu Asn Leu Tyr Asp Pro Asn Lys Tyr Val 
        1235                1240                1245            
Asp Val Asn Asn Val Gly Ile Arg Gly Tyr Met Tyr Leu Lys Gly Pro 
    1250                1255                1260                
Arg Gly Ser Val Met Thr Thr Asn Ile Tyr Leu Asn Ser Ser Leu Tyr 
1265                1270                1275                1280
Arg Gly Thr Lys Phe Ile Ile Lys Lys Tyr Ala Ser Gly Asn Lys Asp 
                1285                1290                1295    
Asn Ile Val Arg Asn Asn Asp Arg Val Tyr Ile Asn Val Val Val Lys 
            1300                1305                1310        
Asn Lys Glu Tyr Arg Leu Ala Thr Asn Ala Ser Gln Ala Gly Val Glu 
        1315                1320                1325            
Lys Ile Leu Ser Ala Leu Glu Ile Pro Asp Val Gly Asn Leu Ser Gln 
    1330                1335                1340                
Val Val Val Met Lys Ser Lys Asn Asp Gln Gly Ile Thr Asn Lys Cys 
1345                1350                1355                1360
Lys Met Asn Leu Gln Asp Asn Asn Gly Asn Asp Ile Gly Phe Ile Gly 
                1365                1370                1375    
Phe His Gln Phe Asn Asn Ile Ala Lys Leu Val Ala Ser Asn Trp Tyr 
            1380                1385                1390        
Asn Arg Gln Ile Glu Arg Ser Ser Arg Thr Leu Gly Cys Ser Trp Glu 
        1395                1400                1405            
Phe Ile Pro Val Asp Asp Gly Trp Gly Glu Arg Pro Leu Ala Ser Pro 
    1410                1415                1420                
Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro 
1425                1430                1435                1440
Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala 
                1445                1450                1455    
Pro Ser Ala Pro Ala Ala Ser Pro Ala Ala Pro Ala Pro Ala Ser Pro 
            1460                1465                1470        
Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala Ser Pro Ala Ala Pro Ala 
        1475                1480                1485            
Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro Ala Ala Ser Pro 
    1490                1495                1500                
Ala Ala Pro Ala Pro Ala Ser Pro Ala Ala Pro Ala Pro Ser Ala Pro 
1505                1510                1515                1520
Ala Gly Asp Leu Val Pro Arg Gly Ser Ala Asn Ser Ser Ser Val Asp 
                1525                1530                1535    
Lys Leu Trp Ser His Pro Gln Phe Glu Lys 
            1540                1545    

<210> 4
<211> 4641
<212> DNA
<213> Artificial Sequence


<220> 
<223> PAS100-BoNT/A (D102F T109R K340M I348L N353M K356R) -PAS100
      including His.tag (nucleic acid sequence)

<400> 4
atgggtagca gccatcatca tcaccatcat ggtagcctgg ttccgcgtag ctcttctgca       60

agtccggcag caccggcacc ggcttcacca gctgcaccag cacctagcgc accggcagca      120

tctccagcag cccctgcacc ggcaagccct gcagctccag caccgtcagc accagcagca      180

agcccagctg ctcctgctcc agcgagccca gcagcgccag ctcctagtgc ccctgctgcc      240

tctcctgctg ctccggcacc agcaagtcct gctgcgcctg caccgagtgc tccggctgct      300

agtcctgccg caccagctcc ggctagtcca gctgctccag ccccttcagc ccctgcagca      360

ccatttgtga acaagcagtt taactataag gacccggtga acggtgtgga tatcgcgtat      420

atcaaaatcc cgaatgcggg ccagatgcaa ccagtcaagg cgttcaagat tcataacaag      480

atttgggtta ttccggaacg tgataccttc accaatccgg aagaaggcga tttaaatccg      540

ccgccagaag ccaaacaagt gccggtgagc tactatgata gcacgtatct tagcaccgat      600

aatgaaaaag acaattacct gaagggcgtg accaagttgt tcgagcgcat ctacagtacc      660

ttcttaggcc gcatgttgtt gcgtagcatc gttcgcggta tcccgttctg gggcggctcg      720

accattgata ccgagttgaa agtcattgac acgaactgta tcaatgttat ccaaccggac      780

ggcagttatc gcagcgagga gttaaatttg gtcatcatcg gtccaagcgc agatattatt      840

cagttcgaat gcaagagctt cggccatgag gtcttgaatt tgacgcgcaa cggttacggc      900

agcacccaat acatccgctt tagcccggat ttcacctttg gcttcgagga gagcttggag      960

gtggacacca acccgctgtt aggtgccggc aaattcgcaa ccgacccggc agtgacgttg     1020

gcgcacgaat tgattcatgc gggtcaccgc ttatacggta tcgcgatcaa tccgaatcgc     1080

gtctttaaag tcaataccaa cgcgtactac gaaatgagcg gcttagaggt tagctttgaa     1140

gaattacgca ccttcggtgg ccacgacgcc aagttcatcg acagcctgca ggaaaatgag     1200

ttccgcttgt actattacaa taaattcaag gacatcgcga gcaccttaaa taaagcaaag     1260

agcattgtgg gcaccaccgc aagcttgcag tacatgaaga acgtatttaa ggaaaaatat     1320

ttgttgtcgg aggataccag cgggaaattc agcgtcgata agctgaaatt cgacatgttg     1380

tataaaatgc tgaccgagct gtacaccgag gatatgttcg tccgtttttt taaggtgtta     1440

aatcgtaaga cctatttaaa ctttgataaa gcggtgttta aaattaatat cgtgccgaag     1500

gtgaattaca ccatctacga tggtttcaat ttacgcaaca cgaatctggc ggcgaatttt     1560

aatggccaaa acaccgaaat taacaacatg aactttacga agttaaagaa tttcacgggc     1620

ttattcgaat tctacaagtt attatgcgtg cgcggcatca ttaccagcaa ggcaggtgcg     1680

ggcaagtcct tggttccgcg tggcagcgcc ggcgccggcg cgctcaatga tctgtgtatt     1740

aaagtcaata actgggacct gttcttcagc ccgagcgagg ataactttac caacgactta     1800

aacaaaggcg aggagatcac gagcgatacg aacatcgagg cggcggagga aaatattagc     1860

ctggacctca ttcagcagta ctatctgacg ttcaattttg acaatgagcc ggagaacatc     1920

agcattgaaa atctcagcag cgacatcatc ggtcagttgg aactgatgcc gaacattgaa     1980

cgctttccga acggcaaaaa atatgaactg gacaagtata ccatgttcca ttacttacgc     2040

gcacaggaat ttgagcacgg caagagccgc attgcgctga ccaatagcgt taacgaggcc     2100

ttgttaaatc cgagccgtgt ctacacgttc ttcagcagcg attatgtcaa aaaagtgaac     2160

aaggcgaccg aagccgcgat gtttttgggc tgggtcgagc aattggttta cgattttacc     2220

gacgaaacca gcgaggtgag cacgaccgac aaaattgcag atatcaccat catcattccg     2280

tacatcggtc cggcgctcaa tatcggcaat atgttataca aggacgactt tgtgggcgcg     2340

ctgatcttta gcggcgcggt tatcttatta gaattcatcc cggagatcgc aatcccggtc     2400

ttgggcacct ttgcgttggt gagctatatc gcgaataaag tgctcacggt ccaaaccatc     2460

gataacgcgc tcagcaagcg taatgagaaa tgggacgagg tttataagta tatcgtgacc     2520

aactggttag caaaagtcaa tacgcagatc gatctcatcc gcaaaaaaat gaaagaagcc     2580

ttggaaaatc aagcggaggc aaccaaagcc atcattaatt accagtataa ccaatatacc     2640

gaagaagaaa aaaacaatat caacttcaat atcgatgatt tgagcagcaa actgaacgag     2700

agcattaaca aagcgatgat taacatcaac aagttcttga atcaatgcag cgtgagctat     2760

ctcatgaaca gcatgatccc gtatggcgtc aaacgcttgg aagattttga cgccagcctg     2820

aaagatgcgc tcctcaagta tatttatgac aaccgcggca ccctcattgg ccaggtggac     2880

cgcttgaagg ataaagtgaa caatacgctc agcacggata tcccgttcca gctgagcaag     2940

tacgtcgaca accagcgctt actgagcacc tttaccgagt atatcaagaa catcattaat     3000

accagcatcc tcaacttgcg ctatgagagc aatcacctga tcgacctcag ccgctacgcc     3060

agcaagatca acatcggcag caaggtcaat ttcgacccga tcgataagaa tcagatccaa     3120

ttgtttaacc tggaaagcag caagatcgag gttatcttga agaacgcgat tgtgtacaac     3180

agcatgtacg agaactttag cacgagcttc tggattcgta tcccgaagta tttcaatagc     3240

attagcctga ataacgaata taccattatc aactgcatgg aaaataatag cggctggaag     3300

gtgagcttaa attacggcga gatcatttgg accttacagg atacccaaga aatcaaacag     3360

cgcgtcgtct ttaagtatag ccagatgatc aacatcagcg attacatcaa ccgctggatc     3420

ttcgtgacca tcaccaataa tcgcttgaat aatagcaaga tttacatcaa tggtcgcttg     3480

attgatcaaa aaccgatcag caatctcggt aatatccatg ccagcaataa catcatgttt     3540

aagttagacg gttgccgcga tacccaccgc tatatctgga tcaagtattt taacttattt     3600

gataaggaac tcaacgaaaa ggaaattaaa gacttatatg acaatcagag caatagcggc     3660

atcctgaagg atttctgggg cgactacctg cagtacgata agccgtacta tatgttgaac     3720

ttgtatgacc cgaacaaata tgtcgatgtg aacaatgtgg gtattcgtgg ctatatgtac     3780

ttaaagggcc cgcgtggtag cgtgatgacc acgaatattt acttaaacag cagcttatac     3840

cgcggcacga agtttattat caagaagtat gccagcggca acaaggacaa tatcgtccgc     3900

aacaacgacc gtgtgtatat taacgtggtg gtgaagaata aagagtaccg cttggccacg     3960

aatgcgagcc aggcgggcgt ggaaaaaatc ttgagcgcgt tggagatccc ggacgtcggc     4020

aacctcagcc aggttgtggt gatgaagtct aaaaacgacc agggcatcac gaacaagtgc     4080

aaaatgaatt tgcaagataa caacggcaac gacatcggct ttattggttt tcaccagttc     4140

aataacatcg ccaaactcgt ggccagcaat tggtataacc gccaaattga acgcagcagc     4200

cgcacgctcg gctgtagctg ggagttcatc ccggtggacg atggctgggg cgagcgcccg     4260

ctcgcaagtc cggcagcacc ggcaccggct tcaccagctg caccagcacc tagcgcaccg     4320

gcagcatctc cagcagcccc tgcaccggca agccctgcag ctccagcacc gtcagcacca     4380

gcagcaagcc cagctgctcc tgctccagcg agcccagcag cgccagctcc tagtgcccct     4440

gctgcctctc ctgctgctcc ggcaccagca agtcctgctg cgcctgcacc gagtgctccg     4500

gctgctagtc ctgccgcacc agctccggct agtccagctg ctccagcccc ttcagcccct     4560

gcaggagatc tggtgccacg cggttccgcg aattcgagct ccgtcgacaa gctttggagc     4620

cacccgcagt tcgaaaaata a                                               4641


<210> 5
<211> 4641
<212> DNA
<213> Artificial Sequence


<220> 
<223> PAS100-BoNT/A (G169I, T220R, P239M, S254T) -PAS100 including
      His.tag) (nucleic acid sequence)

<400> 5
atgggtagca gccatcatca tcaccatcat ggtagcctgg ttccgcgtag ctcttctgca       60

agtccggcag caccggcacc ggcttcacca gctgcaccag cacctagcgc accggcagca      120

tctccagcag cccctgcacc ggcaagccct gcagctccag caccgtcagc accagcagca      180

agcccagctg ctcctgctcc agcgagccca gcagcgccag ctcctagtgc ccctgctgcc      240

tctcctgctg ctccggcacc agcaagtcct gctgcgcctg caccgagtgc tccggctgct      300

agtcctgccg caccagctcc ggctagtcca gctgctccag ccccttcagc ccctgcagca      360

ccatttgtga acaagcagtt taactataag gacccggtga acggtgtgga tatcgcgtat      420

atcaaaatcc cgaatgcggg ccagatgcaa ccagtcaagg cgttcaagat tcataacaag      480

atttgggtta ttccggaacg tgataccttc accaatccgg aagaaggcga tttaaatccg      540

ccgccagaag ccaaacaagt gccggtgagc tactatgata gcacgtatct tagcaccgat      600

aatgaaaaag acaattacct gaagggcgtg accaagttgt tcgagcgcat ctacagtacc      660

gacttaggcc gcatgttgtt gacgagcatc gttcgcggta tcccgttctg gggcggctcg      720

accattgata ccgagttgaa agtcattgac acgaactgta tcaatgttat ccaaccggac      780

ggcagttatc gcagcgagga gttaaatttg gtcatcatcg gtccaagcgc agatattatt      840

cagttcgaat gcaagagctt catccatgag gtcttgaatt tgacgcgcaa cggttacggc      900

agcacccaat acatccgctt tagcccggat ttcacctttg gcttcgagga gagcttggag      960

gtggacacca acccgctgtt aggtgccggc aaattcgcaa ccgacccggc agtgcgcttg     1020

gcgcacgaat tgattcatgc gggtcaccgc ttatacggta tcgcgatcaa tatgaatcgc     1080

gtctttaaag tcaataccaa cgcgtactac gaaatgaccg gcttagaggt tagctttgaa     1140

gaattacgca ccttcggtgg ccacgacgcc aagttcatcg acagcctgca ggaaaatgag     1200

ttccgcttgt actattacaa taaattcaag gacatcgcga gcaccttaaa taaagcaaag     1260

agcattgtgg gcaccaccgc aagcttgcag tacatgaaga acgtatttaa ggaaaaatat     1320

ttgttgtcgg aggataccag cgggaaattc agcgtcgata agctgaaatt cgacaaattg     1380

tataaaatgc tgaccgagat ttacaccgag gataacttcg tcaagttttt taaggtgtta     1440

aatcgtaaga cctatttaaa ctttgataaa gcggtgttta aaattaatat cgtgccgaag     1500

gtgaattaca ccatctacga tggtttcaac ttaagcaaca cgaatctggc ggcgaatttt     1560

aatggccaaa acaccgaaat taacaacatg aactttacga agttaaagaa tttcacgggc     1620

ttattcgaat tctacaagtt attatgcgtg cgcggcatca ttaccagcaa ggcaggtgcg     1680

ggcaagtcct tggttccgcg tggcagcgcc ggcgccggcg cgctcaatga tctgtgtatt     1740

aaagtcaata actgggacct gttcttcagc ccgagcgagg ataactttac caacgactta     1800

aacaaaggcg aggagatcac gagcgatacg aacatcgagg cggcggagga aaatattagc     1860

ctggacctca ttcagcagta ctatctgacg ttcaattttg acaatgagcc ggagaacatc     1920

agcattgaaa atctcagcag cgacatcatc ggtcagttgg aactgatgcc gaacattgaa     1980

cgctttccga acggcaaaaa atatgaactg gacaagtata ccatgttcca ttacttacgc     2040

gcacaggaat ttgagcacgg caagagccgc attgcgctga ccaatagcgt taacgaggcc     2100

ttgttaaatc cgagccgtgt ctacacgttc ttcagcagcg attatgtcaa aaaagtgaac     2160

aaggcgaccg aagccgcgat gtttttgggc tgggtcgagc aattggttta cgattttacc     2220

gacgaaacca gcgaggtgag cacgaccgac aaaattgcag atatcaccat catcattccg     2280

tacatcggtc cggcgctcaa tatcggcaat atgttataca aggacgactt tgtgggcgcg     2340

ctgatcttta gcggcgcggt tatcttatta gaattcatcc cggagatcgc aatcccggtc     2400

ttgggcacct ttgcgttggt gagctatatc gcgaataaag tgctcacggt ccaaaccatc     2460

gataacgcgc tcagcaagcg taatgagaaa tgggacgagg tttataagta tatcgtgacc     2520

aactggttag caaaagtcaa tacgcagatc gatctcatcc gcaaaaaaat gaaagaagcc     2580

ttggaaaatc aagcggaggc aaccaaagcc atcattaatt accagtataa ccaatatacc     2640

gaagaagaaa aaaacaatat caacttcaat atcgatgatt tgagcagcaa actgaacgag     2700

agcattaaca aagcgatgat taacatcaac aagttcttga atcaatgcag cgtgagctat     2760

ctcatgaaca gcatgatccc gtatggcgtc aaacgcttgg aagattttga cgccagcctg     2820

aaagatgcgc tcctcaagta tatttatgac aaccgcggca ccctcattgg ccaggtggac     2880

cgcttgaagg ataaagtgaa caatacgctc agcacggata tcccgttcca gctgagcaag     2940

tacgtcgaca accagcgctt actgagcacc tttaccgagt atatcaagaa catcattaat     3000

accagcatcc tcaacttgcg ctatgagagc aatcacctga tcgacctcag ccgctacgcc     3060

agcaagatca acatcggcag caaggtcaat ttcgacccga tcgataagaa tcagatccaa     3120

ttgtttaacc tggaaagcag caagatcgag gttatcttga agaacgcgat tgtgtacaac     3180

agcatgtacg agaactttag cacgagcttc tggattcgta tcccgaagta tttcaatagc     3240

attagcctga ataacgaata taccattatc aactgcatgg aaaataatag cggctggaag     3300

gtgagcttaa attacggcga gatcatttgg accttacagg atacccaaga aatcaaacag     3360

cgcgtcgtct ttaagtatag ccagatgatc aacatcagcg attacatcaa ccgctggatc     3420

ttcgtgacca tcaccaataa tcgcttgaat aatagcaaga tttacatcaa tggtcgcttg     3480

attgatcaaa aaccgatcag caatctcggt aatatccatg ccagcaataa catcatgttt     3540

aagttagacg gttgccgcga tacccaccgc tatatctgga tcaagtattt taacttattt     3600

gataaggaac tcaacgaaaa ggaaattaaa gacttatatg acaatcagag caatagcggc     3660

atcctgaagg atttctgggg cgactacctg cagtacgata agccgtacta tatgttgaac     3720

ttgtatgacc cgaacaaata tgtcgatgtg aacaatgtgg gtattcgtgg ctatatgtac     3780

ttaaagggcc cgcgtggtag cgtgatgacc acgaatattt acttaaacag cagcttatac     3840

cgcggcacga agtttattat caagaagtat gccagcggca acaaggacaa tatcgtccgc     3900

aacaacgacc gtgtgtatat taacgtggtg gtgaagaata aagagtaccg cttggccacg     3960

aatgcgagcc aggcgggcgt ggaaaaaatc ttgagcgcgt tggagatccc ggacgtcggc     4020

aacctcagcc aggttgtggt gatgaagtct aaaaacgacc agggcatcac gaacaagtgc     4080

aaaatgaatt tgcaagataa caacggcaac gacatcggct ttattggttt tcaccagttc     4140

aataacatcg ccaaactcgt ggccagcaat tggtataacc gccaaattga acgcagcagc     4200

cgcacgctcg gctgtagctg ggagttcatc ccggtggacg atggctgggg cgagcgcccg     4260

ctcgcaagtc cggcagcacc ggcaccggct tcaccagctg caccagcacc tagcgcaccg     4320

gcagcatctc cagcagcccc tgcaccggca agccctgcag ctccagcacc gtcagcacca     4380

gcagcaagcc cagctgctcc tgctccagcg agcccagcag cgccagctcc tagtgcccct     4440

gctgcctctc ctgctgctcc ggcaccagca agtcctgctg cgcctgcacc gagtgctccg     4500

gctgctagtc ctgccgcacc agctccggct agtccagctg ctccagcccc ttcagcccct     4560

gcaggagatc tggtgccacg cggttccgcg aattcgagct ccgtcgacaa gctttggagc     4620

cacccgcagt tcgaaaaata a                                               4641


