SEQUENCE LISTING
<110> University of North Carolina at Greensboro
<120> THERAPEUTIC HUMAN MAST CELLS, COMPOSITIONS, AND METHODS OF TREATING A TUMOR COMPRISING ADMINISTERING AUTOLOGOUS MAST CELLS FROM ADIPOSE TISSUE
<130> UNCG6002
<150> US 62/527,566
<151> 2017-06-30
<160> 16
<210> 1
<211> 20
<212> RNA
<213> Artificial sequence
<220>
<223> beta actin Forward prime
<400> 1
CACTCTTCCAGCCTTCCTTC
<210> 2
<211> 20
<212> RNA
<213> Artificial sequence
<220>
<223> beta actin Reverse primer
<400> 2
GTACAGGTCTTTGCGGATGT
<210> 3
<211> 20
<212> RNA
<213> Artificial sequence
<220>
<223> 18s Forward primer
Page 1
Sequence_Listing_UNCG6001
<400> 3
GCCGCTAGAGGTGAAATTCT
<210> 4
<211> 20
<212> RNA
<213> Artificial sequence
<220>
<223> 18s Reverse primer
<400> 4
TCGGAACTACGACGGTATCT
<210> 5
<211> 28
<212> RNA
<213> Artificial sequence
<220>
<223> Tryptase Forward primer
<400> 5
GCAAAATACCACCTTGGCGCCTACACGG
<210> 6
<211> 24
<212> RNA
<213> Artificial sequence
<220>
<223> Tryptase Reverse primer
<400> 6
GTGACACGGGTGTAGATGCCAGGC
<210> 7
<211> 20
<212> RNA
<213> Artificial sequence
<220>
<223> Chymase Forward primer
<400> 7
CCTGCTGCTCTTTCTCTTGT
Page 2
Sequence_Listing_UNCG6001
<210> 8
<211> 22
<212> RNA
<213> Artificial sequence
<220>
<223> Chymase Reverse primer
<400> 8
GCTCCAAGGGTGACTGTTATAG
<210> 9
<211> 20
<212> RNA
<213> Artificial sequence
<220>
<223> c-KIT Forward primer
<400> 9
TCTATGCTCTCGCACCTTTC
<210> 10
<211> 20
<212> RNA
<213> Artificial sequence
<220>
<223> c-KIT Reverse primer
<400> 10
GACTCATGGGCTTGGGAATA
<210> 11
<211> 22
<212> RNA
<213> Artificial sequence
<220>
<223> FcR1a Forward primer
<400> 11
CCTTGAACCCTCCATGGAATAG
Page 3
Sequence_Listing_UNCG6001
<210> 12
<211> 22
<212> RNA
<213> Artificial sequence
<220>
<223> FcR1a Reverse primer
<400> 12
CACTTTGCCCGTACAGTAGTAG
<210> 13
<211> 1953
<212> mRNA
<213> homo sapiens
<223> TNF-related apoptosis-inducing ligand (TRAIL) (NM_003810)
<400> 13
acagaaccca gaaaaacaac tcattcgctt tcatttcctc actgactata aaagaataga 60
gaaggaaggg cttcagtgac cggctgcctg gctgacttac agcagtcaga ctctgacagg 120
atcatggcta tgatggaggt ccagggggga cccagcctgg gacagacctg cgtgctgatc 180
gtgatcttca cagtgctcct gcagtctctc tgtgtggctg taacttacgt gtactttacc 240
aacgagctga agcagatgca ggacaagtac tccaaaagtg gcattgcttg tttcttaaaa 300
gaagatgaca gttattggga ccccaatgac gaagagagta tgaacagccc ctgctggcaa 360
gtcaagtggc aactccgtca gctcgttaga aagatgattt tgagaacctc tgaggaaacc 420
atttctacag ttcaagaaaa gcaacaaaat atttctcccc tagtgagaga aagaggtcct 480
cagagagtag cagctcacat aactgggacc agaggaagaa gcaacacatt gtcttctcca 540
aactccaaga atgaaaaggc tctgggccgc aaaataaact cctgggaatc atcaaggagt 600
gggcattcat tcctgagcaa cttgcacttg aggaatggtg aactggtcat ccatgaaaaa 660
gggttttact acatctattc ccaaacatac tttcgatttc aggaggaaat aaaagaaaac 720
acaaagaacg acaaacaaat ggtccaatat atttacaaat acacaagtta tcctgaccct 780
atattgttga tgaaaagtgc tagaaatagt tgttggtcta aagatgcaga atatggactc 840
tattccatct atcaaggggg aatatttgag cttaaggaaa atgacagaat ttttgtttct 901
gtaacaaatg agcacttgat agacatggac catgaagcca gtttttttgg ggccttttta 960
gttggctaac tgacctggaa agaaaaagca ataacctcaa agtgactatt cagttttcag 1020
gatgatacac tatgaagatg tttcaaaaaa tctgaccaaa acaaacaaac agaaaacaga 1080
aaacaaaaaa acctctatgc aatctgagta gagcagccac aaccaaaaaa ttctacaaca 1140
cacactgttc tgaaagtgac tcacttatcc caagagaatg aaattgctga aagatctttc 1200
aggactctac ctcatatcag tttgctagca gaaatctaga agactgtcag cttccaaaca 1260
ttaatgcaat ggttaacatc ttctgtcttt ataatctact ccttgtaaag actgtagaag 1320
aaagagcaac aatccatctc tcaagtagtg tatcacagta gtagcctcca ggtttcctta 1380
agggacaaca tccttaagtc aaaagagaga agaggcacca ctaaaagatc gcagtttgcc 1440
tggtgcagtg gctcacacct gtaatcccaa cattttggga acccaaggtg ggtagatcac 1500
gagatcaaga gatcaagacc atagtgacca acatagtgaa accccatctc tactgaaagt 1560
Page 4
Sequence_Listing_UNCG6001
acaaaaatta gctgggtgtg ttggcacatg cctgtagtcc cagctacttg agaggctgag 1620
gcaagagaat tgtttgaacc cgggaggcag aggttgcagt gtggtgagat catgccacta 1680
cactccagcc tggcgacaga gcgagacttg gtttcaaaaa aaaaaaaaaa aaaaacttca 1740
gtaagtacgt gttatttttt tcaataaaat tctattacag tatgtcatgt ttgctgtagt 1800
gctcatattt attgttgttt ttgttttagt actcacttgt ttcataatat caagattact 1860
aaaaatgggg gaaaagactt ctaatctttt tttcataata tctttgacac atattacaga 1920
agaaataaat ttcttacttt taatttaata tga 1953
<210> 14
<211> 4018
<212> mRNA
<213> homo sapiens
<223> Telomerase reverse transcriptase (hTERT) (NM_198253)
<400> 14
caggcagcgc tgcgtcctgc tgcgcacgtg ggaagccctg gccccggcca cccccgcgat 60
gccgcgcgct ccccgctgcc gagccgtgcg ctccctgctg cgcagccact accgcgaggt 120
gctgccgctg gccacgttcg tgcggcgcct ggggccccag ggctggcggc tggtgcagcg 180
cggggacccg gcggctttcc gcgcgctggt ggcccagtgc ctggtgtgcg tgccctggga 240
cgcacggccg ccccccgccg ccccctcctt ccgccaggtg tcctgcctga aggagctggt 300
ggcccgagtg ctgcagaggc tgtgcgagcg cggcgcgaag aacgtgctgg ccttcggctt 360
cgcgctgctg gacggggccc gcgggggccc ccccgaggcc ttcaccacca gcgtgcgcag 420
ctacctgccc aacacggtga ccgacgcact gcgggggagc ggggcgtggg ggctgctgct 480
gcgccgcgtg ggcgacgacg tgctggttca cctgctggca cgctgcgcgc tctttgtgct 540
ggtggctccc agctgcgcct accaggtgtg cgggccgccg ctgtaccagc tcggcgctgc 600
cactcaggcc cggcccccgc cacacgctag tggaccccga aggcgtctgg gatgcgaacg 660
ggcctggaac catagcgtca gggaggccgg ggtccccctg ggcctgccag ccccgggtgc 720
gaggaggcgc gggggcagtg ccagccgaag tctgccgttg cccaagaggc ccaggcgtgg 780
cgctgcccct gagccggagc ggacgcccgt tgggcagggg tcctgggccc acccgggcag 840
gacgcgtgga ccgagtgacc gtggtttctg tgtggtgtca cctgccagac ccgccgaaga 900
agccacctct ttggagggtg cgctctctgg cacgcgccac tcccacccat ccgtgggccg 960
ccagcaccac gcgggccccc catccacatc gcggccacca cgtccctggg acacgccttg 1020
tcccccggtg tacgccgaga ccaagcactt cctctactcc tcaggcgaca aggagcagct 1080
gcggccctcc ttcctactca gctctctgag gcccagcctg actggcgctc ggaggctcgt 1140
ggagaccatc tttctgggtt ccaggccctg gatgccaggg actccccgca ggttgccccg 1200
cctgccccag cgctactggc aaatgcggcc cctgtttctg gagctgcttg ggaaccacgc 1260
gcagtgcccc tacggggtgc tcctcaagac gcactgcccg ctgcgagctg cggtcacccc 1320
agcagccggt gtctgtgccc gggagaagcc ccagggctct gtggcggccc ccgaggagga 1381
ggacacagac ccccgtcgcc tggtgcagct gctccgccag cacagcagcc cctggcaggt 1441
gtacggcttc gtgcgggcct gcctgcgccg gctggtgccc ccaggcctct ggggctccag 1501
gcacaacgaa cgccgcttcc tcaggaacac caagaagttc atctccctgg ggaagcatgc 1560
caagctctcg ctgcaggagc tgacgtggaa gatgagcgtg cgggactgcg cttggctgcg 1620
caggagccca ggggttggct gtgttccggc cgcagagcac cgtctgcgtg aggagatcct 1680
ggccaagttc ctgcactggc tgatgagtgt gtacgtcgtc gagctgctca ggtctttctt 1740
ttatgtcacg gagaccacgt ttcaaaagaa caggctcttt ttctaccgga agagtgtctg 1800
gagcaagttg caaagcattg gaatcagaca gcacttgaag agggtgcagc tgcgggagct 1860
Page 5
Sequence_Listing_UNCG6001
gtcggaagca gaggtcaggc agcatcggga agccaggccc gccctgctga cgtccagact 1920
ccgcttcatc cccaagcctg acgggctgcg gccgattgtg aacatggact acgtcgtggg 1980
agccagaacg ttccgcagag aaaagagggc cgagcgtctc acctcgaggg tgaaggcact 2040
gttcagcgtg ctcaactacg agcgggcgcg gcgccccggc ctcctgggcg cctctgtgct 2100
gggcctggac gatatccaca gggcctggcg caccttcgtg ctgcgtgtgc gggcccagga 2160
cccgccgcct gagctgtact ttgtcaaggt ggatgtgacg ggcgcgtacg acaccatccc 2220
ccaggacagg ctcacggagg tcatcgccag catcatcaaa ccccagaaca cgtactgcgt 2280
gcgtcggtat gccgtggtcc agaaggccgc ccatgggcac gtccgcaagg ccttcaagag 2340
ccacgtctct accttgacag acctccagcc gtacatgcga cagttcgtgg ctcacctgca 2400
ggagaccagc ccgctgaggg atgccgtcgt catcgagcag agctcctccc tgaatgaggc 2460
cagcagtggc ctcttcgacg tcttcctacg cttcatgtgc caccacgccg tgcgcatcag 2520
gggcaagtcc tacgtccagt gccaggggat cccgcagggc tccatcctct ccacgctgct 2580
ctgcagcctg tgctacggcg acatggagaa caagctgttt gcggggattc ggcgggacgg 2640
gctgctcctg cgtttggtgg atgatttctt gttggtgaca cctcacctca cccacgcgaa 2700
aaccttcctc aggaccctgg tccgaggtgt ccctgagtat ggctgcgtgg tgaacttgcg 2760
gaagacagtg gtgaacttcc ctgtagaaga cgaggccctg ggtggcacgg cttttgttca 2820
gatgccggcc cacggcctat tcccctggtg cggcctgctg ctggataccc ggaccctgga 2880
ggtgcagagc gactactcca gctatgcccg gacctccatc agagccagtc tcaccttcaa 2940
ccgcggcttc aaggctggga ggaacatgcg tcgcaaactc tttggggtct tgcggctgaa 3000
gtgtcacagc ctgtttctgg atttgcaggt gaacagcctc cagacggtgt gcaccaacat 3060
ctacaagatc ctcctgctgc aggcgtacag gtttcacgca tgtgtgctgc agctcccatt 3120
tcatcagcaa gtttggaaga accccacatt tttcctgcgc gtcatctctg acacggcctc 3180
cctctgctac tccatcctga aagccaagaa cgcagggatg tcgctggggg ccaagggcgc 3240
cgccggccct ctgccctccg aggccgtgca gtggctgtgc caccaagcat tcctgctcaa 3300
gctgactcga caccgtgtca cctacgtgcc actcctgggg tcactcagga cagcccagac 3360
gcagctgagt cggaagctcc cggggacgac gctgactgcc ctggaggccg cagccaaccc 3420
ggcactgccc tcagacttca agaccatcct ggactgatgg ccacccgccc acagccaggc 3480
cgagagcaga caccagcagc cctgtcacgc cgggctctac gtcccaggga gggaggggcg 3540
gcccacaccc aggcccgcac cgctgggagt ctgaggcctg agtgagtgtt tggccgaggc 3600
ctgcatgtcc ggctgaaggc tgagtgtccg gctgaggcct gagcgagtgt ccagccaagg 3660
gctgagtgtc cagcacacct gccgtcttca cttccccaca ggctggcgct cggctccacc 3720
ccagggccag cttttcctca ccaggagccc ggcttccact ccccacatag gaatagtcca 3780
tccccagatt cgccattgtt cacccctcgc cctgccctcc tttgccttcc acccccacca 3840
tccaggtgga gaccctgaga aggaccctgg gagctctggg aatttggagt gaccaaaggt 3900
gtgccctgta cacaggcgag gaccctgcac ctggatgggg gtccctgtgg gtcaaattgg 3960
ggggaggtgc tgtgggagta aaatactgaa tatatgagtt tttcagtttt gaaaaaaa 4018
<210> 15
<211> 391
<212> dna
<213> Macaca mulatta polyomavirus 1
<223> SV40 Large T Antigen (M99347)
<400> 15
tttatgtttc aggttcaggg ggaggtggtt ttttaaagca agtaaaacct ctacagatgt 60
gatatggctg atcatgaaca gactgtgagg actgtgaggg ctgaggggcc tgaaatgagc 120
Page 6
Sequence_Listing_UNCG6001
cttgggactg tgaatcaatg cctgtttcat gccctgagtc ttccatgttc ttctccccac 180
catcttcatt tttatcagca ttttcctcat catcatcact gtttcttagc caatttaaaa 240
ctccagttcc catagccaca ttaaacttca ttttttgata cactgacaaa ctaaactctt 300
tgtccaatct ctctttccac tccacaattc tgccctgaat actttgagca aactcagcca 360
caggtctgta ccaaattaac ataagaagca a 391
<210> 16
<211> 2123
<212> mRNA
<213> homo sapiens
<223> T-cell leukemia homeobox 1 (TLX1)(NM_005521)
<400> 16
tcccagagtc aacagcgagc gagcagccgg agcggggaag cagaagccag agaggggaag 60
aatacggcgc cccctctctc cctcccctcc cccttctact ttagcctttc tgcgcacttc 120
gcttccaagt ctccgcgcag ccaggagccg ctgttgcctc ccagcccctg ctagctgccc 180
cccgagccga gcgcagcgag cgccgccgcc cgggcccccc ggtggggcca gggccagcat 240
ggagcacctg ggtccgcacc acctccaccc gggtcacgca gagcccatta gcttcggcat 300
cgaccagatc ctcaacagcc cggaccaggg tggctgcatg ggacccgcct cgcgcctcca 360
ggacggagaa tacggccttg gctgcttggt cggaggcgcc tacacttacg gcggcggggg 420
ctccgcggcc gcgacggggg ctggaggagc gggggcctat ggtactggag gtcccggcgg 480
ccccggaggc ccggcaggcg gcggcggcgc ctgcagcatg ggtcctctga ccggctccta 540
caacgtgaac atggccttgg caggcggccc cggtcctggc ggcggcggcg gcagcagcgg 600
cggtgccggg gcactcagcg ctgcgggggt aatccgggtg ccggcacaca ggccgctcgc 660
cggagccgtg gcccaccccc agcccctggc caccggcttg cccaccgtgc cctctgtgcc 720
tgccatgccg ggcgtcaaca acctcactgg cctcaccttc ccctggatgg agagtaaccg 780
cagatacaca aaggacaggt tcacaggtca cccctatcag aaccggacgc cccccaagaa 840
gaagaagccg cgcacgtcct tcacacgcct gcagatctgc gagctggaga agcgcttcca 900
ccgccagaag tacctggcct cggccgagcg cgccgccctg gccaaggcgc tcaaaatgac 960
cgatgcgcag gtcaaaacct ggttccagaa ccggcggaca aagtggagac ggcagactgc 1020
ggaggaacgg gaggccgaga ggcagcaagc gaaccgcatc ctcctgcagt tgcagcagga 1080
ggccttccag aagagcctgg cacagccgct gcccgctgac cctctgtgcg tgcacaactc 1140
gtcgctcttc gccctgcaga atctgcagcc gtggtctgac gactcgacca aaatcactag 1200
cgtcacgtcg gtggcgtcgg cctgcgagtg agcctgccca ttctgccctg tgggacccca 1260
ggcccactca ggggtcactg aggcctgaga cccaggactc ctccccaccc tcctggcctc 1320
agactgcacc caggagggga acactgccct cgcacggccc cgaagggccc ccacatttgt 1380
gccgacactg ttctcccttc ggtggaagag ctcaagggac aaggacacgc gcccccctcc 1440
cagaggcgtc ccgcacctgt ctgaactgtt aagaaatctg tttttgttta tttcatttta 1500
ttttaatttt taacgtggga ttcagagaaa ggcaagggag gtaagggagg aggagcttct 1560
ggggtcccca gggctgtcat ctgaatttgc cctgggaaac cccttctctg tgacccactt 1620
ctcatcacac acatggaaac ccataggccc acacacaggt ggtgtcactg tccctcctgg 1680
tgtcacccca gagccacaca tgggcatcta tgggagagtg tcaaccagac agagggtcac 1740
agtgtttaca ctttggacct tacgatcagg cacaggtcag gggtgacaca gactcatcct 1800
gaacagcatg gcactccctc cagcacaaac acaaggtcat ggccacactg tgacacacta 1860
caccacacac aacagccaac agctacaaca gcctcacttg gtctgccagg cccccaccac 1920
acatcccagc ccaatccagg tacgcacaga caggttttca cataaatgca gcccatttct 1980
Page 7
Sequence_Listing_UNCG6001
ccagaaccca tttgaggggt gggggggtgt taatttatgc acttataagg tgttttctgt 2040
gtaaccattt tataaagtgc ttgtgtaatt tatgtgaaaa aaataaataa aagcctccgg 2100
atccggaaaa aaaaaaaaaa aaa 2123
Page 8