                         SEQUENCE LISTING

<110>  Yale University
       Pyle, Anna Marie
       Zhao, Chen
 
<120>  Improved Reverse Transcriptase and Methods of Use

<130>  047162-5243-00-WO.607468

<150>  62/525,337
<151>  2017-06-27

<160>  30    

<170>  PatentIn version 3.5

<210>  1
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  1
accatatttc catccaccaa gcgc                                              24


<210>  2
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  2
taataggtga ggtttcaatg                                                   20


<210>  3
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  3
gtctccgctg gtgtgag                                                      17


<210>  4
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  4
aaggaacagt tagctatgga gtg                                               23


<210>  5
<211>  65
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized


<220>
<221>  misc_feature
<222>  (27)..(51)
<223>  sequence barcode region, n represents any nucleotide

<400>  5
ctacacgacg ctcttccgat ctctgtnnnn nnnnnnnnnn ngattatagg acatttaggt       60

cgtac                                                                   65


<210>  6
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized


<220>
<221>  misc_feature
<222>  (26)..(50)
<223>  sequence barcode region, n represents any nucleotide

<400>  6
cagacgtgtg ctcttccgat cggtannnnn nnnnnnnnnn acatttctaa ctggaagtca       60

agc                                                                     63


<210>  7
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized


<220>
<221>  misc_feature
<222>  (26)..(40)
<223>  n is a, c, g, or t

<400>  7
cagacgtgtg ctcttccgat cggtannnnn nnnnnnnnnn acatttctaa ctggaagtca       60

agc                                                                     63


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  8
cagacgtgtg ctcttccgat c                                                 21


<210>  9
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  9
caagcagaag acggcatacg agatacatcg gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  10
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  10
caagcagaag acggcatacg agattggtca gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  11
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  11
caagcagaag acggcatacg agatacagtg gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  12
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  12
caagcagaag acggcatacg agatcagatc gtgactggag ttcagacgtg tgctcttccg       60

atct                                                                    64


<210>  13
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  13
aatgatacgg cgaccaccga gatctacact ctttccctac acgacgctct tccgatc          57


<210>  14
<211>  427
<212>  PRT
<213>  Eubacterium rectale

<400>  14

Met Asp Thr Ser Asn Leu Met Glu Gln Ile Leu Ser Ser Asp Asn Leu 
1               5                   10                  15      


Asn Arg Ala Tyr Leu Gln Val Val Arg Asn Lys Gly Ala Glu Gly Val 
            20                  25                  30          


Asp Gly Met Lys Tyr Thr Glu Leu Lys Glu His Leu Ala Lys Asn Gly 
        35                  40                  45              


Glu Thr Ile Lys Gly Gln Leu Arg Thr Arg Lys Tyr Lys Pro Gln Pro 
    50                  55                  60                  


Ala Arg Arg Val Glu Ile Pro Lys Pro Asp Gly Gly Val Arg Asn Leu 
65                  70                  75                  80  


Gly Val Pro Thr Val Thr Asp Arg Phe Ile Gln Gln Ala Ile Ala Gln 
                85                  90                  95      


Val Leu Thr Pro Ile Tyr Glu Glu Gln Phe His Asp His Ser Tyr Gly 
            100                 105                 110         


Phe Arg Pro Asn Arg Cys Ala Gln Gln Ala Ile Leu Thr Ala Leu Asn 
        115                 120                 125             


Ile Met Asn Asp Gly Asn Asp Trp Ile Val Asp Ile Asp Leu Glu Lys 
    130                 135                 140                 


Phe Phe Asp Thr Val Asn His Asp Lys Leu Met Thr Leu Ile Gly Arg 
145                 150                 155                 160 


Thr Ile Lys Asp Gly Asp Val Ile Ser Ile Val Arg Lys Tyr Leu Val 
                165                 170                 175     


Ser Gly Ile Met Ile Asp Asp Glu Tyr Glu Asp Ser Ile Val Gly Thr 
            180                 185                 190         


Pro Gln Gly Gly Asn Leu Ser Pro Leu Leu Ala Asn Ile Met Leu Asn 
        195                 200                 205             


Glu Leu Asp Lys Glu Met Glu Lys Arg Gly Leu Asn Phe Val Arg Tyr 
    210                 215                 220                 


Ala Asp Asp Cys Ile Ile Met Val Gly Ser Glu Met Ser Ala Asn Arg 
225                 230                 235                 240 


Val Met Arg Asn Ile Ser Arg Phe Ile Glu Glu Lys Leu Gly Leu Lys 
                245                 250                 255     


Val Asn Met Thr Lys Ser Lys Val Asp Arg Pro Ser Gly Leu Lys Tyr 
            260                 265                 270         


Leu Gly Phe Gly Phe Tyr Phe Asp Pro Arg Ala His Gln Phe Lys Ala 
        275                 280                 285             


Lys Pro His Ala Lys Ser Val Ala Lys Phe Lys Lys Arg Met Lys Glu 
    290                 295                 300                 


Leu Thr Cys Arg Ser Trp Gly Val Ser Asn Ser Tyr Lys Val Glu Lys 
305                 310                 315                 320 


Leu Asn Gln Leu Ile Arg Gly Trp Ile Asn Tyr Phe Lys Ile Gly Ser 
                325                 330                 335     


Met Lys Thr Leu Cys Lys Glu Leu Asp Ser Arg Ile Arg Tyr Arg Leu 
            340                 345                 350         


Arg Met Cys Ile Trp Lys Gln Trp Lys Thr Pro Gln Asn Gln Glu Lys 
        355                 360                 365             


Asn Leu Val Lys Leu Gly Ile Asp Arg Asn Thr Ala Arg Arg Val Ala 
    370                 375                 380                 


Tyr Thr Gly Lys Arg Ile Ala Tyr Val Cys Asn Lys Gly Ala Val Asn 
385                 390                 395                 400 


Val Ala Ile Ser Asn Lys Arg Leu Ala Ser Phe Gly Leu Ile Ser Met 
                405                 410                 415     


Leu Asp Tyr Tyr Ile Glu Lys Cys Val Thr Cys 
            420                 425         


<210>  15
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, E.r. maturase mut1

<400>  15

Met Asp Thr Ser Asn Leu Met Glu Gln Ile Leu Ser Ser Asp Asn Leu 
1               5                   10                  15      


Asn Arg Ala Tyr Leu Gln Val Val Arg Asn Lys Gly Ala Glu Gly Val 
            20                  25                  30          


Asp Gly Met Lys Tyr Thr Glu Leu Lys Glu His Leu Ala Lys Asn Gly 
        35                  40                  45              


Glu Thr Ile Lys Gly Gln Leu Arg Thr Ala Ala Tyr Ala Pro Gln Pro 
    50                  55                  60                  


Ala Arg Arg Val Glu Ile Pro Lys Pro Asp Gly Gly Val Arg Asn Leu 
65                  70                  75                  80  


Gly Val Pro Thr Val Thr Asp Arg Phe Ile Gln Gln Ala Ile Ala Gln 
                85                  90                  95      


Val Leu Thr Pro Ile Tyr Glu Glu Gln Phe His Asp His Ser Tyr Gly 
            100                 105                 110         


Phe Arg Pro Asn Arg Cys Ala Gln Gln Ala Ile Leu Thr Ala Leu Asn 
        115                 120                 125             


Ile Met Asn Asp Gly Asn Asp Trp Ile Val Asp Ile Asp Leu Glu Lys 
    130                 135                 140                 


Phe Phe Asp Thr Val Asn His Asp Lys Leu Met Thr Leu Ile Gly Arg 
145                 150                 155                 160 


Thr Ile Ala Asp Gly Asp Val Ile Ser Ile Val Arg Lys Tyr Leu Val 
                165                 170                 175     


Ser Gly Ile Met Ile Asp Asp Glu Tyr Glu Asp Ser Ile Val Gly Thr 
            180                 185                 190         


Pro Gln Gly Gly Asn Leu Ser Pro Leu Leu Ala Asn Ile Met Leu Asn 
        195                 200                 205             


Glu Leu Asp Lys Glu Met Glu Lys Arg Gly Leu Asn Phe Val Arg Tyr 
    210                 215                 220                 


Ala Asp Asp Cys Ile Ile Met Val Gly Ser Glu Met Ser Ala Asn Arg 
225                 230                 235                 240 


Val Met Arg Asn Ile Ser Arg Phe Ile Glu Glu Lys Leu Gly Leu Lys 
                245                 250                 255     


Val Asn Met Thr Lys Ser Lys Val Asp Arg Pro Ser Gly Leu Lys Tyr 
            260                 265                 270         


Leu Gly Phe Gly Phe Tyr Phe Asp Pro Arg Ala His Gln Phe Lys Ala 
        275                 280                 285             


Lys Pro His Ala Lys Ser Val Ala Lys Phe Lys Lys Arg Met Lys Glu 
    290                 295                 300                 


Leu Thr Cys Arg Ser Trp Gly Val Ser Asn Ser Tyr Lys Val Glu Lys 
305                 310                 315                 320 


Leu Asn Gln Leu Ile Arg Gly Trp Ile Asn Tyr Phe Lys Ile Gly Ser 
                325                 330                 335     


Met Lys Thr Leu Cys Lys Glu Leu Asp Ser Arg Ile Arg Tyr Arg Leu 
            340                 345                 350         


Arg Met Cys Ile Trp Lys Gln Trp Lys Thr Pro Gln Asn Gln Glu Lys 
        355                 360                 365             


Asn Leu Val Lys Leu Gly Ile Asp Arg Asn Thr Ala Arg Arg Val Ala 
    370                 375                 380                 


Tyr Thr Gly Lys Arg Ile Ala Tyr Val Cys Asn Lys Gly Ala Val Asn 
385                 390                 395                 400 


Val Ala Ile Ser Asn Lys Arg Leu Ala Ser Phe Gly Leu Ile Ser Met 
                405                 410                 415     


Leu Asp Tyr Tyr Ile Glu Lys Cys Val Thr Cys 
            420                 425         


<210>  16
<211>  426
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, E.r. maturase mut2

<400>  16

Met Asp Thr Ser Asn Leu Met Glu Gln Ile Leu Ser Ser Asp Asn Leu 
1               5                   10                  15      


Asn Arg Ala Tyr Leu Gln Val Val Arg Asn Lys Gly Ala Glu Gly Val 
            20                  25                  30          


Asp Gly Met Lys Tyr Thr Glu Leu Lys Glu His Leu Ala Lys Asn Gly 
        35                  40                  45              


Glu Thr Ile Lys Gly Gln Leu Arg Thr Arg Lys Tyr Lys Pro Gln Pro 
    50                  55                  60                  


Ala Arg Arg Val Glu Ile Pro Lys Pro Asp Gly Gly Val Arg Asn Leu 
65                  70                  75                  80  


Gly Val Pro Thr Val Thr Asp Arg Phe Ile Gln Gln Ala Ile Ala Gln 
                85                  90                  95      


Val Leu Thr Pro Ile Tyr Glu Glu Gln Phe His Asp His Ser Tyr Gly 
            100                 105                 110         


Phe Arg Pro Asn Arg Cys Ala Gln Gln Ala Ile Leu Thr Ala Leu Asn 
        115                 120                 125             


Ile Met Asn Asp Gly Asn Asp Trp Ile Val Asp Ile Asp Leu Glu Lys 
    130                 135                 140                 


Phe Phe Asp Thr Val Asn His Asp Lys Leu Met Thr Leu Ile Gly Arg 
145                 150                 155                 160 


Thr Ile Lys Asp Gly Asp Val Ile Ser Ile Val Arg Lys Tyr Leu Val 
                165                 170                 175     


Ser Gly Ile Met Ile Asp Asp Glu Tyr Glu Asp Ser Ile Val Gly Thr 
            180                 185                 190         


Pro Gln Gly Gly Asn Leu Ser Pro Leu Leu Ala Asn Ile Met Leu Asn 
        195                 200                 205             


Glu Leu Asp Lys Glu Met Glu Ala Ala Gly Leu Asn Phe Val Arg Tyr 
    210                 215                 220                 


Ala Asp Asp Cys Ile Ile Met Val Gly Ser Glu Met Ser Ala Asn Arg 
225                 230                 235                 240 


Val Met Arg Asn Ile Ser Arg Phe Ile Glu Glu Lys Leu Gly Leu Lys 
                245                 250                 255     


Val Asn Met Thr Lys Ser Lys Val Asp Arg Pro Ser Gly Leu Lys Tyr 
            260                 265                 270         


Leu Gly Phe Gly Phe Tyr Phe Asp Pro Arg Ala His Gln Phe Lys Ala 
        275                 280                 285             


Lys Pro His Ala Lys Ser Val Ala Lys Phe Lys Lys Arg Met Lys Glu 
    290                 295                 300                 


Leu Thr Cys Arg Ser Trp Gly Val Ser Asn Ser Tyr Lys Val Glu Lys 
305                 310                 315                 320 


Leu Asn Gln Leu Ile Arg Gly Trp Ile Asn Tyr Phe Lys Ile Gly Ser 
                325                 330                 335     


Met Lys Thr Leu Cys Lys Glu Leu Asp Ser Arg Ile Arg Tyr Arg Leu 
            340                 345                 350         


Arg Met Cys Ile Trp Lys Gln Trp Lys Thr Pro Gln Asn Gln Glu Lys 
        355                 360                 365             


Asn Leu Val Lys Leu Gly Ile Asp Arg Asn Thr Ala Arg Arg Val Ala 
    370                 375                 380                 


Tyr Thr Gly Lys Arg Ile Ala Tyr Val Cys Asn Lys Gly Ala Val Asn 
385                 390                 395                 400 


Val Ala Ile Ser Asn Lys Arg Leu Ala Ser Phe Gly Leu Ile Ser Met 
                405                 410                 415     


Leu Asp Tyr Tyr Ile Glu Lys Cys Val Thr 
            420                 425     


<210>  17
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, E.r. maturase mut1+mut2

<400>  17

Met Asp Thr Ser Asn Leu Met Glu Gln Ile Leu Ser Ser Asp Asn Leu 
1               5                   10                  15      


Asn Arg Ala Tyr Leu Gln Val Val Arg Asn Lys Gly Ala Glu Gly Val 
            20                  25                  30          


Asp Gly Met Lys Tyr Thr Glu Leu Lys Glu His Leu Ala Lys Asn Gly 
        35                  40                  45              


Glu Thr Ile Lys Gly Gln Leu Arg Thr Ala Ala Tyr Ala Pro Gln Pro 
    50                  55                  60                  


Ala Arg Arg Val Glu Ile Pro Lys Pro Asp Gly Gly Val Arg Asn Leu 
65                  70                  75                  80  


Gly Val Pro Thr Val Thr Asp Arg Phe Ile Gln Gln Ala Ile Ala Gln 
                85                  90                  95      


Val Leu Thr Pro Ile Tyr Glu Glu Gln Phe His Asp His Ser Tyr Gly 
            100                 105                 110         


Phe Arg Pro Asn Arg Cys Ala Gln Gln Ala Ile Leu Thr Ala Leu Asn 
        115                 120                 125             


Ile Met Asn Asp Gly Asn Asp Trp Ile Val Asp Ile Asp Leu Glu Lys 
    130                 135                 140                 


Phe Phe Asp Thr Val Asn His Asp Lys Leu Met Thr Leu Ile Gly Arg 
145                 150                 155                 160 


Thr Ile Ala Asp Gly Asp Val Ile Ser Ile Val Arg Lys Tyr Leu Val 
                165                 170                 175     


Ser Gly Ile Met Ile Asp Asp Glu Tyr Glu Asp Ser Ile Val Gly Thr 
            180                 185                 190         


Pro Gln Gly Gly Asn Leu Ser Pro Leu Leu Ala Asn Ile Met Leu Asn 
        195                 200                 205             


Glu Leu Asp Lys Glu Met Glu Ala Ala Gly Leu Asn Phe Val Arg Tyr 
    210                 215                 220                 


Ala Asp Asp Cys Ile Ile Met Val Gly Ser Glu Met Ser Ala Asn Arg 
225                 230                 235                 240 


Val Met Arg Asn Ile Ser Arg Phe Ile Glu Glu Lys Leu Gly Leu Lys 
                245                 250                 255     


Val Asn Met Thr Lys Ser Lys Val Asp Arg Pro Ser Gly Leu Lys Tyr 
            260                 265                 270         


Leu Gly Phe Gly Phe Tyr Phe Asp Pro Arg Ala His Gln Phe Lys Ala 
        275                 280                 285             


Lys Pro His Ala Lys Ser Val Ala Lys Phe Lys Lys Arg Met Lys Glu 
    290                 295                 300                 


Leu Thr Cys Arg Ser Trp Gly Val Ser Asn Ser Tyr Lys Val Glu Lys 
305                 310                 315                 320 


Leu Asn Gln Leu Ile Arg Gly Trp Ile Asn Tyr Phe Lys Ile Gly Ser 
                325                 330                 335     


Met Lys Thr Leu Cys Lys Glu Leu Asp Ser Arg Ile Arg Tyr Arg Leu 
            340                 345                 350         


Arg Met Cys Ile Trp Lys Gln Trp Lys Thr Pro Gln Asn Gln Glu Lys 
        355                 360                 365             


Asn Leu Val Lys Leu Gly Ile Asp Arg Asn Thr Ala Arg Arg Val Ala 
    370                 375                 380                 


Tyr Thr Gly Lys Arg Ile Ala Tyr Val Cys Asn Lys Gly Ala Val Asn 
385                 390                 395                 400 


Val Ala Ile Ser Asn Lys Arg Leu Ala Ser Phe Gly Leu Ile Ser Met 
                405                 410                 415     


Leu Asp Tyr Tyr Ile Glu Lys Cys Val Thr Cys 
            420                 425         


<210>  18
<211>  427
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, E.r. maturase mut3

<400>  18

Met Asp Thr Ser Asn Leu Met Glu Gln Ile Leu Ser Ser Asp Asn Leu 
1               5                   10                  15      


Asn Arg Ala Tyr Leu Gln Val Val Arg Asn Lys Gly Ala Glu Gly Val 
            20                  25                  30          


Asp Gly Met Lys Tyr Thr Glu Leu Lys Glu His Leu Ala Lys Asn Gly 
        35                  40                  45              


Glu Thr Ile Lys Gly Gln Leu Arg Thr Arg Lys Tyr Lys Pro Gln Pro 
    50                  55                  60                  


Ala Arg Arg Val Glu Ile Pro Lys Pro Asp Gly Gly Val Arg Asn Leu 
65                  70                  75                  80  


Gly Val Pro Thr Val Thr Asp Arg Phe Ile Gln Gln Ala Ile Ala Gln 
                85                  90                  95      


Val Leu Thr Pro Ile Tyr Glu Glu Gln Phe His Asp His Ser Tyr Gly 
            100                 105                 110         


Phe Arg Pro Asn Arg Cys Ala Gln Gln Ala Ile Leu Thr Ala Leu Asn 
        115                 120                 125             


Ile Met Asn Asp Gly Asn Asp Trp Ile Val Asp Ile Asp Leu Glu Lys 
    130                 135                 140                 


Phe Phe Asp Thr Val Asn His Asp Lys Leu Met Thr Leu Ile Gly Arg 
145                 150                 155                 160 


Thr Ile Lys Asp Gly Asp Val Ile Ser Ile Val Arg Lys Tyr Leu Val 
                165                 170                 175     


Ser Gly Ile Met Ile Asp Asp Glu Tyr Glu Asp Ser Ile Val Gly Thr 
            180                 185                 190         


Pro Gln Gly Gly Asn Leu Ser Pro Leu Leu Ala Asn Ile Met Leu Asn 
        195                 200                 205             


Glu Leu Asp Lys Glu Met Glu Lys Arg Gly Leu Asn Phe Val Arg Tyr 
    210                 215                 220                 


Ala Asp Asp Cys Ile Ile Met Val Gly Ser Glu Met Ser Ala Asn Arg 
225                 230                 235                 240 


Val Met Arg Asn Ile Ser Arg Phe Ile Glu Glu Lys Leu Gly Leu Lys 
                245                 250                 255     


Val Asn Met Thr Lys Ser Lys Val Asp Arg Pro Ser Gly Leu Lys Tyr 
            260                 265                 270         


Leu Gly Phe Gly Phe Tyr Phe Asp Pro Arg Ala His Gln Phe Lys Ala 
        275                 280                 285             


Lys Pro His Ala Lys Ser Val Ala Lys Phe Lys Lys Arg Met Lys Glu 
    290                 295                 300                 


Leu Thr Cys Arg Ser Trp Gly Val Ser Asn Ser Tyr Lys Val Glu Lys 
305                 310                 315                 320 


Leu Asn Gln Leu Ile Arg Gly Trp Ile Asn Tyr Phe Lys Ile Gly Ser 
                325                 330                 335     


Met Ala Thr Leu Cys Ala Glu Leu Asp Ser Arg Ile Arg Tyr Arg Leu 
            340                 345                 350         


Ala Met Cys Ile Trp Lys Gln Trp Lys Thr Pro Gln Asn Gln Glu Lys 
        355                 360                 365             


Asn Leu Val Lys Leu Gly Ile Asp Arg Asn Thr Ala Arg Arg Val Ala 
    370                 375                 380                 


Tyr Thr Gly Lys Arg Ile Ala Tyr Val Cys Asn Lys Gly Ala Val Asn 
385                 390                 395                 400 


Val Ala Ile Ser Asn Lys Arg Leu Ala Ser Phe Gly Leu Ile Ser Met 
                405                 410                 415     


Leu Asp Tyr Tyr Ile Glu Lys Cys Val Thr Cys 
            420                 425         


<210>  19
<211>  418
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized, E.r. maturase delta loop

<400>  19

Met Asp Thr Ser Asn Leu Met Glu Gln Ile Leu Ser Ser Asp Asn Leu 
1               5                   10                  15      


Asn Arg Ala Tyr Leu Gln Val Val Arg Asn Lys Gly Ala Glu Gly Val 
            20                  25                  30          


Asp Gly Met Lys Tyr Thr Glu Leu Lys Glu His Leu Ala Lys Asn Gly 
        35                  40                  45              


Glu Thr Ile Lys Gly Gln Leu Arg Thr Arg Lys Tyr Lys Pro Gln Pro 
    50                  55                  60                  


Ala Arg Arg Val Glu Ile Pro Lys Pro Asp Gly Gly Val Arg Asn Leu 
65                  70                  75                  80  


Gly Val Pro Thr Val Thr Asp Arg Phe Ile Gln Gln Ala Ile Ala Gln 
                85                  90                  95      


Val Leu Thr Pro Ile Tyr Glu Glu Gln Phe His Asp His Ser Tyr Gly 
            100                 105                 110         


Phe Arg Pro Asn Arg Cys Ala Gln Gln Ala Ile Leu Thr Ala Leu Asn 
        115                 120                 125             


Ile Met Asn Asp Gly Asn Asp Trp Ile Val Asp Ile Asp Leu Glu Lys 
    130                 135                 140                 


Phe Phe Asp Thr Val Asn His Asp Lys Leu Met Thr Leu Ile Gly Arg 
145                 150                 155                 160 


Thr Ile Lys Asp Gly Asp Val Ile Ser Ile Val Arg Lys Tyr Leu Val 
                165                 170                 175     


Ser Gly Ile Met Ile Gly Gly Pro Gln Gly Gly Asn Leu Ser Pro Leu 
            180                 185                 190         


Leu Ala Asn Ile Met Leu Asn Glu Leu Asp Lys Glu Met Glu Lys Arg 
        195                 200                 205             


Gly Leu Asn Phe Val Arg Tyr Ala Asp Asp Cys Ile Ile Met Val Gly 
    210                 215                 220                 


Ser Glu Met Ser Ala Asn Arg Val Met Arg Asn Ile Ser Arg Phe Ile 
225                 230                 235                 240 


Glu Glu Lys Leu Gly Leu Lys Val Asn Met Thr Lys Ser Lys Val Asp 
                245                 250                 255     


Arg Pro Ser Gly Leu Lys Tyr Leu Gly Phe Gly Phe Tyr Phe Asp Pro 
            260                 265                 270         


Arg Ala His Gln Phe Lys Ala Lys Pro His Ala Lys Ser Val Ala Lys 
        275                 280                 285             


Phe Lys Lys Arg Met Lys Glu Leu Thr Cys Arg Ser Trp Gly Val Ser 
    290                 295                 300                 


Asn Ser Tyr Lys Val Glu Lys Leu Asn Gln Leu Ile Arg Gly Trp Ile 
305                 310                 315                 320 


Asn Tyr Phe Lys Ile Gly Ser Met Lys Thr Leu Cys Lys Glu Leu Asp 
                325                 330                 335     


Ser Arg Ile Arg Tyr Arg Leu Arg Met Cys Ile Trp Lys Gln Trp Lys 
            340                 345                 350         


Thr Pro Gln Asn Gln Glu Lys Asn Leu Val Lys Leu Gly Ile Asp Arg 
        355                 360                 365             


Asn Thr Ala Arg Arg Val Ala Tyr Thr Gly Lys Arg Ile Ala Tyr Val 
    370                 375                 380                 


Cys Asn Lys Gly Ala Val Asn Val Ala Ile Ser Asn Lys Arg Leu Ala 
385                 390                 395                 400 


Ser Phe Gly Leu Ile Ser Met Leu Asp Tyr Tyr Ile Glu Lys Cys Val 
                405                 410                 415     


Thr Cys 
        


<210>  20
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  20

Met Ile Asp Asp Glu Tyr Glu Asp Ser Ile Val Gly Thr Pro Gln Gly 
1               5                   10                  15      


Gly 
    


<210>  21
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  21

Met Ile Asp Asp Glu Tyr Glu Asp Ser Ile Val 
1               5                   10      


<210>  22
<211>  41
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  22

Gly Lys Arg Ile Ala Tyr Val Cys Asn Lys Gly Ala Val Asn Val Ala 
1               5                   10                  15      


Ile Ser Asn Lys Arg Leu Ala Ser Phe Gly Leu Ile Ser Met Leu Asp 
            20                  25                  30          


Tyr Tyr Ile Glu Lys Cys Val Thr Cys 
        35                  40      


<210>  23
<211>  70
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  23
gucucguauu gcagaaauga caacaucugc cguaaccaau cggguaaaag guggucaaau       60

caagcgagac                                                              70


<210>  24
<211>  463
<212>  PRT
<213>  Chloroflexi bacterium

<400>  24

Met Ser Gly Gln Arg Gln Lys Asn Arg Pro Glu Gln Glu Val Leu Ala 
1               5                   10                  15      


Phe Pro Val Glu Gly Arg Ser Asp Ala Pro Lys Ala Ala Glu Lys Gly 
            20                  25                  30          


Thr Glu Thr Leu Val Ala Lys Arg Lys Thr Glu Ser Leu Ala Gly Thr 
        35                  40                  45              


Glu Arg Leu Met Glu Glu Val Cys Glu Leu Glu Asn Ser Lys Gln Ala 
    50                  55                  60                  


Leu Gln Arg Val Lys Ala Asn Lys Gly Ser Pro Gly Val Asp Gly Met 
65                  70                  75                  80  


Thr Val Asp Glu Leu Pro Glu Tyr Leu Lys Gln Tyr Gly Pro Glu Ile 
                85                  90                  95      


Gly Glu Gln Leu Arg Asn Gly Thr Tyr Gln Pro Gln Pro Val Arg Arg 
            100                 105                 110         


Val Glu Ile Pro Lys Pro Asp Gly Ser Gly Met Arg Lys Leu Gly Ile 
        115                 120                 125             


Pro Cys Val Leu Asp Arg Phe Val Gln Gln Ala Val Leu Gln Val Leu 
    130                 135                 140                 


Gln Lys Arg Trp Asp Pro Thr Phe Ser Glu His Ser His Gly Phe Arg 
145                 150                 155                 160 


Pro Gly His Ser Ala Arg Gln Ala Val His Glu Ala Gln Gln Tyr Ile 
                165                 170                 175     


Ala Glu Gly His Gly Trp Val Val Asp Leu Asp Leu Glu Lys Phe Phe 
            180                 185                 190         


Asp Arg Val Asn His Asp Arg Leu Leu Ala Ala Val Ala Glu Arg Val 
        195                 200                 205             


Ala Asp Lys Arg Met Leu Lys Leu Ile Arg Ala Phe Leu Lys Ala Gly 
    210                 215                 220                 


Val Met Glu Asp Gly Leu Val Ser Pro Val Asp Glu Gly Thr Pro Gln 
225                 230                 235                 240 


Gly Gly Pro Leu Ser Pro Leu Leu Ser Asn Leu Val Leu Asp Glu Leu 
                245                 250                 255     


Asp Arg Glu Leu Glu Arg Arg Gly His His Phe Val Arg Tyr Ala Asp 
            260                 265                 270         


Asp Cys Asn Ile Tyr Val Gly Ser Glu Arg Ala Gly Gln Arg Val Met 
        275                 280                 285             


Glu Ser Val Thr His Phe Ile Thr His Arg Leu Lys Leu Lys Val Asn 
    290                 295                 300                 


Gln Ala Lys Ser Ala Val Ala Arg Pro Arg Gln Arg Lys Phe Leu Gly 
305                 310                 315                 320 


Phe Ser Phe Thr Ser Glu Arg Glu Pro Arg Arg Arg Ile Ala Pro Lys 
                325                 330                 335     


Ala Ile Ala Arg Cys Lys Glu Arg Ile Arg Glu Gln Thr Arg Arg Thr 
            340                 345                 350         


Arg Gly Ile Ser Leu Ala Gln Met Val Lys Glu Ile Ala Thr Tyr Leu 
        355                 360                 365             


Arg Gly Trp Leu Gly Tyr Phe Gly Asp Cys Gln Thr Pro Ser Val Leu 
    370                 375                 380                 


Gln Arg Leu Glu Ser Trp Leu Arg Arg Arg Leu Arg Ser Val Val Trp 
385                 390                 395                 400 


Lys Gln Trp Lys Arg Gly Arg Thr Arg Phe Arg Glu Leu Arg Lys Arg 
                405                 410                 415     


Gly Ile Arg Lys Asp Leu Ala Ala Gln Thr Ala Gly Ser Pro Gln Gly 
            420                 425                 430         


Pro Trp Arg Ile Ala Asn Ser Pro Ala Leu Ser Ile Ala Leu Ser Asn 
        435                 440                 445             


Ala Tyr Phe Ala Glu Leu Gly Leu Pro Pro Met Val Val Arg Ser 
    450                 455                 460             


<210>  25
<211>  419
<212>  PRT
<213>  Bacillus licheniformis

<400>  25

Met Leu Met Asn Met Ile Leu Ser Arg Glu Asn Leu Ile Lys Ala Leu 
1               5                   10                  15      


Lys Arg Val Glu Lys Asn Lys Gly Ser His Gly Ile Asp Gly Met Ser 
            20                  25                  30          


Val Lys Ser Leu Arg Arg His Leu Tyr Glu Asn Trp Glu Thr Leu Cys 
        35                  40                  45              


Asn Ser Leu Arg Asn Gly Thr Tyr Gln Pro Asn Pro Val Arg Arg Val 
    50                  55                  60                  


Glu Ile Pro Lys Pro Asn Gly Gly Val Arg Leu Leu Gly Ile Pro Asn 
65                  70                  75                  80  


Val Thr Asp Arg Phe Ile Gln Gln Ala Ile Ala Gln Val Leu Thr Pro 
                85                  90                  95      


Leu Phe Asp Pro Thr Phe Ser Glu His Ser Tyr Gly Phe Arg Pro Asn 
            100                 105                 110         


Lys Arg Gly His Asp Ala Val Arg Lys Ala Arg Gln Tyr Ile Ser Glu 
        115                 120                 125             


Gly Tyr Arg Trp Val Ile Asp Met Asp Leu Glu Lys Phe Phe Asp Lys 
    130                 135                 140                 


Val Asn His Asp Lys Leu Met Gly Ile Leu Ala Ser Arg Ile Gln Asp 
145                 150                 155                 160 


Arg Leu Val Leu Lys Leu Ile Arg Lys Tyr Leu Gln Ala Gly Ile Met 
                165                 170                 175     


Ile Asn Gly Val Val Tyr Asp Ala Glu Glu Gly Thr Pro Gln Gly Gly 
            180                 185                 190         


Pro Leu Ser Pro Leu Leu Ser Asn Ile Leu Leu Asp Lys Leu Asp Lys 
        195                 200                 205             


Glu Leu Glu Ser Arg Gly His Lys Phe Val Arg Tyr Ala Asp Asp Cys 
    210                 215                 220                 


Asn Ile Tyr Met Lys Ser Lys Lys Ala Gly Glu Arg Val Met Asn Ser 
225                 230                 235                 240 


Ile Thr His Phe Ile Glu Glu Lys Leu Lys Leu Lys Val Asn Lys Glu 
                245                 250                 255     


Lys Ser Ala Val Asp Arg Pro Trp Arg Arg Lys Phe Leu Gly Phe Ser 
            260                 265                 270         


Phe Thr Val Asn Lys Lys Pro Lys Val Arg Ile Ala Asn Glu Ser Ile 
        275                 280                 285             


Lys Arg Leu Lys Ala Lys Ile Arg Glu Phe Thr Ser Arg Ser Lys Pro 
    290                 295                 300                 


Ile Pro Met Asp Val Arg Ile Glu Lys Leu Asn Arg Tyr Leu Thr Gly 
305                 310                 315                 320 


Trp Cys Gly Tyr Phe Ala Leu Ala Asp Thr Pro Ser Lys Phe Lys Glu 
                325                 330                 335     


Phe Asp Glu Trp Ile Arg Arg Arg Leu Arg Met Ile Glu Trp Lys Gln 
            340                 345                 350         


Trp Lys Lys Pro Met Thr Arg Val Arg Lys Leu Lys Gly Leu Gly Val 
        355                 360                 365             


Pro Asp Gln Lys Ala Tyr Glu Trp Gly Asn Ser Arg Lys Lys Tyr Trp 
    370                 375                 380                 


Arg Ile Ala Cys Ser Pro Ile Leu Asn Lys Thr Leu Asp Asn Ser Tyr 
385                 390                 395                 400 


Trp Ser Asn Arg Gly Leu Lys Ser Leu Tyr Gln Arg Tyr Glu Phe Leu 
                405                 410                 415     


Arg Gln Thr 
            


<210>  26
<211>  419
<212>  PRT
<213>  Geobacillus stearothermophilus

<400>  26

Met Trp Met Lys Gln Val Leu Ser Arg Glu Asn Leu Leu Arg Ala Leu 
1               5                   10                  15      


Lys Gln Val Glu Lys Asn Lys Gly Ser His Gly Thr Asp Gly Met Ser 
            20                  25                  30          


Val Lys Asp Leu Arg Arg His Leu Val Glu His Trp Asp Ala Ile Arg 
        35                  40                  45              


His Ala Leu Glu Glu Gly Thr Tyr Glu Pro Cys Pro Val Arg Arg Val 
    50                  55                  60                  


Glu Ile Pro Lys Pro Asn Gly Gly Val Arg Leu Leu Gly Ile Pro Thr 
65                  70                  75                  80  


Val Thr Asp Arg Phe Ile Gln Gln Ala Ile Ala Gln Val Leu Thr Pro 
                85                  90                  95      


Ile Phe Asp Pro Ser Phe Leu Glu His Ser Tyr Gly Phe Arg Pro Gly 
            100                 105                 110         


Arg Arg Gly His Asp Ala Val Lys Lys Ala Lys Gln Tyr Ile Gln Glu 
        115                 120                 125             


Gly Tyr Thr Trp Val Val Asp Ile Asp Leu Glu Lys Phe Phe Asp Arg 
    130                 135                 140                 


Val Asn His Asp Lys Leu Met Gly Ile Leu Ala Lys Arg Ile Pro Asp 
145                 150                 155                 160 


Lys Ile Leu Leu Lys Leu Ile Arg Lys Tyr Leu Gln Ala Gly Val Met 
                165                 170                 175     


Ile Asn Gly Val Val Met Glu Thr Gln Glu Gly Thr Pro Gln Gly Gly 
            180                 185                 190         


Pro Leu Ser Pro Leu Leu Ser Asn Ile Leu Leu Asp Glu Leu Asp Lys 
        195                 200                 205             


Glu Leu Glu Lys Arg Gly His Lys Phe Val Arg Tyr Ala Asp Asp Cys 
    210                 215                 220                 


Asn Ile Tyr Val Arg Thr Gln Lys Ala Gly Glu Arg Val Met Lys Ser 
225                 230                 235                 240 


Ile Thr Ala Phe Ile Glu Lys Lys Leu Arg Leu Lys Val Asn Glu Thr 
                245                 250                 255     


Lys Ser Ala Val Asp Arg Pro Trp Arg Arg Lys Phe Leu Gly Phe Ser 
            260                 265                 270         


Phe Thr Pro Ser Lys Glu Pro Lys Ile Arg Ile Ala Arg Glu Ser Ile 
        275                 280                 285             


Arg Arg Met Lys Gln Arg Ile Arg Thr Met Thr Ser Arg Ser Lys Pro 
    290                 295                 300                 


Ile Pro Met Pro Glu Arg Ile Glu Gln Leu Asn Gln Tyr Ile Leu Gly 
305                 310                 315                 320 


Trp Cys Gly Tyr Phe Ser Leu Ala Glu Thr Pro Ser Val Phe Lys Glu 
                325                 330                 335     


Leu Asp Gly Trp Ile Arg Arg Arg Leu Arg Met Cys Gln Trp Lys Glu 
            340                 345                 350         


Trp Lys Leu Pro Arg Thr Arg Val Arg Lys Leu Gln Ser Leu Gly Val 
        355                 360                 365             


Pro Lys Gln Lys Ala Tyr Glu Trp Gly Asn Thr Arg Lys Lys Tyr Trp 
    370                 375                 380                 


Arg Val Ala Ala Ser Pro Ile Leu His Lys Ala Leu Gly Asn Ser Tyr 
385                 390                 395                 400 


Trp Glu Ser Gln Gly Leu Lys Ser Leu Tyr Gln Arg Tyr Glu Ser Leu 
                405                 410                 415     


Arg Gln Thr 
            


<210>  27
<211>  434
<212>  PRT
<213>  Chloroflexus aurantiacus

<400>  27

Met Leu Val Phe Pro Arg Tyr Ser Gly Pro Ser Leu Ile Glu Gln Val 
1               5                   10                  15      


Cys Thr Val Glu Asn Leu Thr Asn Ala Trp Arg Arg Val Arg Ser Asn 
            20                  25                  30          


Ile Lys Val Ser Arg Arg Thr Arg Ser Ala Gly Ile Asp Gln Ile Thr 
        35                  40                  45              


Leu His Asp Phe Ala Ala Asp Trp Pro Asn Gln Met Val Arg Leu Ala 
    50                  55                  60                  


Glu Glu Leu Arg Asp Gly Ser Tyr Arg Pro Leu Pro Pro Arg Arg Val 
65                  70                  75                  80  


Ala Ile Ala Lys Ala Ser Gly Gly Glu Arg Ala Ile Ala Ile Leu Thr 
                85                  90                  95      


Ile Arg Asp Arg Ile Ala Gln Arg Ala Val Gln Gln Val Leu Thr Pro 
            100                 105                 110         


Leu Phe Glu Pro Leu Phe Leu Asp Cys Ser Tyr Gly Ser Arg Leu Ala 
        115                 120                 125             


Val Gly Val Pro Glu Ala Ile Glu Arg Val Val Arg Tyr Thr Glu Gln 
    130                 135                 140                 


Gly Leu Ile Trp Val Ile Asp Gly Asp Ile Arg Ala Tyr Phe Asp Ser 
145                 150                 155                 160 


Ile Asp His Gly Ile Leu Leu Gly Leu Leu Arg Gln Arg Ile Asp Glu 
                165                 170                 175     


Pro Ala Ile Leu His Leu Ile Ala Gln Trp Leu Ala Val Gly Ser Val 
            180                 185                 190         


His Thr Glu Thr Pro Asp Glu Thr Leu Pro Asp Ser Pro Leu Val Ala 
        195                 200                 205             


Leu Leu Arg Arg Ser Gly Glu Leu Ile His Glu Ala Leu Asn Ala Pro 
    210                 215                 220                 


Ser Asp Pro Leu Pro Thr Ala Tyr Asp Tyr Pro Asp Leu Ser Arg Pro 
225                 230                 235                 240 


Ala Ser Pro His Ser Gly Ile Pro Thr Gly Leu Phe Ala Ala Leu Ser 
                245                 250                 255     


Leu Ala Gln Pro Ala Phe Glu Ile Ala Arg Gln Leu Thr Pro Leu Leu 
            260                 265                 270         


Lys Arg Ile Gly Ala Gln Arg Leu Ala Val Gly Gly Ala Leu Ala Val 
        275                 280                 285             


Gly Thr Val Leu Leu Ser Glu Leu Val His Arg Ala Gln Ala Ser His 
    290                 295                 300                 


Asp Arg Arg Gly Thr Leu Gln Gly Gly Pro Leu Ser Pro Leu Leu Ala 
305                 310                 315                 320 


Asn Ile Tyr Leu His Pro Phe Asp Leu Ala Met Thr Ala His Gly Ala 
                325                 330                 335     


Arg Met Val Arg Phe Val Asp Asp Phe Val Val Met Cys Pro Asp Arg 
            340                 345                 350         


Thr Thr Ala Glu His Thr Leu Val Leu Val Glu Arg Gln Leu Ala Thr 
        355                 360                 365             


Leu Arg Leu Thr Leu Asn Pro Gln Lys Thr Arg Ile Val Ala Tyr Ala 
    370                 375                 380                 


Gly Gly Ile Glu Phe Leu Gly Gln Ala Leu Ala Pro Arg Arg Arg Gln 
385                 390                 395                 400 


Gly Phe Leu His Gly Ile Ser Asp Phe His Ser Ala Glu Gln Arg Leu 
                405                 410                 415     


Arg Glu His Val Glu Arg Leu Arg Arg Pro Asp Lys Pro Pro Thr Ser 
            420                 425                 430         


Asp Arg 
        


<210>  28
<211>  13
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  28
cguaaccaau cgg                                                          13


<210>  29
<211>  15
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  29
ccguaaccaa ucggg                                                        15


<210>  30
<211>  22
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Chemically Synthesized

<400>  30
ugccguaacc aaucggguaa aa                                                22


