			SEQUENCE LISTING

<110> CODEXIS, INC.
      ALEXION PHARMACEUTICALS, INC.
 
<120> T7 RNA POLYMERASE VARIANTS
 
<130> CX8-168USP1
 
<140> NOT YET ASSIGNED
<141> SUBMITTED HEREWITH
 
<160> 40
 
<210> 1
<211> 2649
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP Wild Type
 
<400> 1
atgaacacga ttaacatcgc taagaacgac ttctctgaca tcgaactggc tgctatcccg 60
ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt ggcccttgag 120
catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg tcaacttaaa 180
gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct actccctaag 240
atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg caagcgcccg 300
acagccttcc agttcctgca agaaatcaag ccggaagccg tagcgtacat caccattaag 360
accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt agcaagcgca 420
atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct tgaagctaag 480
cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt ctacaagaaa 540
gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg tggcgaggcg 600
tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat cgagatgctc 660
attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt aggtcaagac 720
tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg tgcaggtgcg 780
ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc gtggactggc 840
attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt gcgtactcac 900
agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta caaagcgatt 960
aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt cgccaacgta 1020
atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg tgaagaactc 1080
ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg gaaacgtgct 1140
gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag ccttgagttc 1200
atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc ttacaacatg 1260
gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa cgatatgacc 1320
aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta ctactggctg 1380
aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc cgttccctga gcgcatcaag 1440
ttcattgagg aaaaccacga gaacatcatg gcttgcgcta agtctccact ggagaacact 1500
tggtgggctg agcaagattc tccgttctgc ttccttgcgt tctgctttga gtacgctggg 1560
gtacagcacc acggcctgag ctataactgc tcccttccgc tggcgtttga cgggtcttgc 1620
tctggcatcc agcacttctc cgcgatgctc cgagatgagg taggtggtcg cgcggttaac 1680
ttgcttccta gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa agtcaacgag 1740
attctacaag cagacgcaat caatgggacc gataacgaag tagttaccgt gaccgatgag 1800
aacactggtg aaatctctga gaaagtcaag ctgggcacta aggcactggc tggtcaatgg 1860
ctggcttacg gtgttactcg cagtgtgact aagcgttcag tcatgacgct ggcttacggg 1920
tccaaagagt tcggcttccg tcaacaagtg ctggaagata ccattcagcc agctattgat 1980
tccggcaagg gtctgatgtt cactcagccg aatcaggctg ctggatacat ggctaagctg 2040
atttgggaat ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa ctggcttaag 2100
tctgctgcta agctgctggc tgctgaggtc aaagataaga agactggaga gattcttcgc 2160
aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca ggaatacaag 2220
aagcctattc agacgcgctt gaacctgatg ttcctcggtc agttccgctt acagcctacc 2280
attaacacca acaaagatag cgagattgat gcacacaaac aggagtctgg tatcgctcct 2340
aactttgtac acagccaaga cggtagccac cttcgtaaga ctgtagtgtg ggcacacgag 2400
aagtacggaa tcgaatcttt tgcactgatt cacgactcct tcggtaccat tccggctgac 2460
gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg acacatatga gtcttgtgat 2520
gtactggctg atttctacga ccagttcgct gaccagttgc acgagtctca attggacaaa 2580
atgccagcac ttccggctaa aggtaacttg aacctccgtg acatcttaga gtcggacttc 2640
gcgttcgcg                                                         2649

 
<210> 2
<211> 883
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP Wild Type
 
<400> 2
Met Asn Thr Ile Asn Ile Ala Lys Asn Asp Phe Ser Asp Ile Glu Leu 
1               5                   10                  15      
Ala Ala Ile Pro Phe Asn Thr Leu Ala Asp His Tyr Gly Glu Arg Leu 
            20                  25                  30          
Ala Arg Glu Gln Leu Ala Leu Glu His Glu Ser Tyr Glu Met Gly Glu 
        35                  40                  45              
Ala Arg Phe Arg Lys Met Phe Glu Arg Gln Leu Lys Ala Gly Glu Val 
    50                  55                  60                  
Ala Asp Asn Ala Ala Ala Lys Pro Leu Ile Thr Thr Leu Leu Pro Lys 
65                  70                  75                  80  
Met Ile Ala Arg Ile Asn Asp Trp Phe Glu Glu Val Lys Ala Lys Arg 
                85                  90                  95      
Gly Lys Arg Pro Thr Ala Phe Gln Phe Leu Gln Glu Ile Lys Pro Glu 
            100                 105                 110         
Ala Val Ala Tyr Ile Thr Ile Lys Thr Thr Leu Ala Cys Leu Thr Ser 
        115                 120                 125             
Ala Asp Asn Thr Thr Val Gln Ala Val Ala Ser Ala Ile Gly Arg Ala 
    130                 135                 140                 
Ile Glu Asp Glu Ala Arg Phe Gly Arg Ile Arg Asp Leu Glu Ala Lys 
145                 150                 155                 160 
His Phe Lys Lys Asn Val Glu Glu Gln Leu Asn Lys Arg Val Gly His 
                165                 170                 175     
Val Tyr Lys Lys Ala Phe Met Gln Val Val Glu Ala Asp Met Leu Ser 
            180                 185                 190         
Lys Gly Leu Leu Gly Gly Glu Ala Trp Ser Ser Trp His Lys Glu Asp 
        195                 200                 205             
Ser Ile His Val Gly Val Arg Cys Ile Glu Met Leu Ile Glu Ser Thr 
    210                 215                 220                 
Gly Met Val Ser Leu His Arg Gln Asn Ala Gly Val Val Gly Gln Asp 
225                 230                 235                 240 
Ser Glu Thr Ile Glu Leu Ala Pro Glu Tyr Ala Glu Ala Ile Ala Thr 
                245                 250                 255     
Arg Ala Gly Ala Leu Ala Gly Ile Ser Pro Met Phe Gln Pro Cys Val 
            260                 265                 270         
Val Pro Pro Lys Pro Trp Thr Gly Ile Thr Gly Gly Gly Tyr Trp Ala 
        275                 280                 285             
Asn Gly Arg Arg Pro Leu Ala Leu Val Arg Thr His Ser Lys Lys Ala 
    290                 295                 300                 
Leu Met Arg Tyr Glu Asp Val Tyr Met Pro Glu Val Tyr Lys Ala Ile 
305                 310                 315                 320 
Asn Ile Ala Gln Asn Thr Ala Trp Lys Ile Asn Lys Lys Val Leu Ala 
                325                 330                 335     
Val Ala Asn Val Ile Thr Lys Trp Lys His Cys Pro Val Glu Asp Ile 
            340                 345                 350         
Pro Ala Ile Glu Arg Glu Glu Leu Pro Met Lys Pro Glu Asp Ile Asp 
        355                 360                 365             
Met Asn Pro Glu Ala Leu Thr Ala Trp Lys Arg Ala Ala Ala Ala Val 
    370                 375                 380                 
Tyr Arg Lys Asp Lys Ala Arg Lys Ser Arg Arg Ile Ser Leu Glu Phe 
385                 390                 395                 400 
Met Leu Glu Gln Ala Asn Lys Phe Ala Asn His Lys Ala Ile Trp Phe 
                405                 410                 415     
Pro Tyr Asn Met Asp Trp Arg Gly Arg Val Tyr Ala Val Ser Met Phe 
            420                 425                 430         
Asn Pro Gln Gly Asn Asp Met Thr Lys Gly Leu Leu Thr Leu Ala Lys 
        435                 440                 445             
Gly Lys Pro Ile Gly Lys Glu Gly Tyr Tyr Trp Leu Lys Ile His Gly 
    450                 455                 460                 
Ala Asn Cys Ala Gly Val Asp Lys Val Pro Phe Pro Glu Arg Ile Lys 
465                 470                 475                 480 
Phe Ile Glu Glu Asn His Glu Asn Ile Met Ala Cys Ala Lys Ser Pro 
                485                 490                 495     
Leu Glu Asn Thr Trp Trp Ala Glu Gln Asp Ser Pro Phe Cys Phe Leu 
            500                 505                 510         
Ala Phe Cys Phe Glu Tyr Ala Gly Val Gln His His Gly Leu Ser Tyr 
        515                 520                 525             
Asn Cys Ser Leu Pro Leu Ala Phe Asp Gly Ser Cys Ser Gly Ile Gln 
    530                 535                 540                 
His Phe Ser Ala Met Leu Arg Asp Glu Val Gly Gly Arg Ala Val Asn 
545                 550                 555                 560 
Leu Leu Pro Ser Glu Thr Val Gln Asp Ile Tyr Gly Ile Val Ala Lys 
                565                 570                 575     
Lys Val Asn Glu Ile Leu Gln Ala Asp Ala Ile Asn Gly Thr Asp Asn 
            580                 585                 590         
Glu Val Val Thr Val Thr Asp Glu Asn Thr Gly Glu Ile Ser Glu Lys 
        595                 600                 605             
Val Lys Leu Gly Thr Lys Ala Leu Ala Gly Gln Trp Leu Ala Tyr Gly 
    610                 615                 620                 
Val Thr Arg Ser Val Thr Lys Arg Ser Val Met Thr Leu Ala Tyr Gly 
625                 630                 635                 640 
Ser Lys Glu Phe Gly Phe Arg Gln Gln Val Leu Glu Asp Thr Ile Gln 
                645                 650                 655     
Pro Ala Ile Asp Ser Gly Lys Gly Leu Met Phe Thr Gln Pro Asn Gln 
            660                 665                 670         
Ala Ala Gly Tyr Met Ala Lys Leu Ile Trp Glu Ser Val Ser Val Thr 
        675                 680                 685             
Val Val Ala Ala Val Glu Ala Met Asn Trp Leu Lys Ser Ala Ala Lys 
    690                 695                 700                 
Leu Leu Ala Ala Glu Val Lys Asp Lys Lys Thr Gly Glu Ile Leu Arg 
705                 710                 715                 720 
Lys Arg Cys Ala Val His Trp Val Thr Pro Asp Gly Phe Pro Val Trp 
                725                 730                 735     
Gln Glu Tyr Lys Lys Pro Ile Gln Thr Arg Leu Asn Leu Met Phe Leu 
            740                 745                 750         
Gly Gln Phe Arg Leu Gln Pro Thr Ile Asn Thr Asn Lys Asp Ser Glu 
        755                 760                 765             
Ile Asp Ala His Lys Gln Glu Ser Gly Ile Ala Pro Asn Phe Val His 
    770                 775                 780                 
Ser Gln Asp Gly Ser His Leu Arg Lys Thr Val Val Trp Ala His Glu 
785                 790                 795                 800 
Lys Tyr Gly Ile Glu Ser Phe Ala Leu Ile His Asp Ser Phe Gly Thr 
                805                 810                 815     
Ile Pro Ala Asp Ala Ala Asn Leu Phe Lys Ala Val Arg Glu Thr Met 
            820                 825                 830         
Val Asp Thr Tyr Glu Ser Cys Asp Val Leu Ala Asp Phe Tyr Asp Gln 
        835                 840                 845             
Phe Ala Asp Gln Leu His Glu Ser Gln Leu Asp Lys Met Pro Ala Leu 
    850                 855                 860                 
Pro Ala Lys Gly Asn Leu Asn Leu Arg Asp Ile Leu Glu Ser Asp Phe 
865                 870                 875                 880 
Ala Phe Ala                                                     
                                                                

 
<210> 3
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> 6His-T7RNAP 
 
<400> 3
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct 1200
cgccgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaagatt ctccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat 1980
accattcagc cagctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 4
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> 6His-T7RNAP
 
<400> 4
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Arg Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Asp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Asp Thr Ile Gln Pro Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

<210> 5
<211> 3060
<212> DNA
<213> Artificial Sequence
 
<220>
<223> DNA template
 
<400> 5
gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 60
cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 120
ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttagccc 180
accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 240
tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 300
cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 360
gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 420
ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 480
cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 540
tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 600
ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 660
ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 720
ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg 780
agagtaggga actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg 840
cgtttctaca aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct 900
gggcggttct gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg 960
ggttttttta tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg 1020
tcactttgct tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact 1080
ttaaataaga tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat 1140
tatttatgat tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa 1200
atctcgaaaa taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat 1260
aatacaaaat ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa 1320
attttgtgtc gcccttgtac ttagtcgctg aataatacga ctcactatag cggaacccaa 1380
gctaagcgcc agaactggca ccttcgggtg ccagttgacg aggtggggtt tatcgagatt 1440
tcggcggatg actcccggtt gttcatcaca accgcaagct tttacttaaa tcattaaggt 1500
gacttagtgg acaaaggtga aagtgtgatg aaacccgacc tggacggagg cgcgcccgag 1560
atgagtaggc tgtcccatca ggggaggaat cggggacggc tgaaaggcga gggcgccgaa 1620
gcgagcagag ttcctcccgc tctgcttggc tgggggtgag gggaataccc ttaccactgt 1680
cgcgaaagcg gagagccgtc caggatcccg tcaaaagggc gacaccccat aattagcccg 1740
ggcgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctg gcagttccct 1800
actctcgcat ggggagtccc cacactacca tcggcgctac ggcgtttcac ttctgagttc 1860
ggcatggggt caggtgggac caccgcgcta ctgccgccag gcaaacaagg ggtgttatga 1920
gccatattca ggtataaatg ggctcgcgat aatgttcaga attggttaat tggttgtaac 1980
actgacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 2040
ataaccctga taaatgcttc aataatattg aaaaaggaag aatatgagta ttcaacattt 2100
ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 2160
aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 2220
actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 2280
gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 2340
agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 2400
cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 2460
catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 2520
aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 2580
gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag cgatggcaac 2640
aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 2700
agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 2760
ctggtttatt gctgataaat ccggagccgg tgagcgtggt tctcgcggta tcatcgcagc 2820
gctggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 2880
aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 2940
gtaaaagcag agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct 3000
taacgtgagt tacgcgcgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 3060

 
 
<210> 6
<211> 334
<212> RNA
<213> Artificial Sequence
 
<220>
<223> RNA Transcript
 
<400> 6
gcggaaccca agcuaagcgc cagaacuggc accuucgggu gccaguugac gagguggggu 60
uuaucgagau uucggcggau gacucccggu uguucaucac aaccgcaagc uuuuacuuaa 120
aucauuaagg ugacuuagug gacaaaggug aaagugugau gaaacccgac cuggacggag 180
gcgcgcccga gaugaguagg cugucccauc aggggaggaa ucggggacgg cugaaaggcg 240
agggcgccga agcgagcaga guuccucccg cucugcuugg cuggggguga ggggaauacc 300
cuuaccacug ucgcgaaagc ggagagccgu ccag                             334

 
<210> 7
<211> 16
<212> RNA
<213> Artificial Sequence
 
<220>
<223> RNA cleavage product
 
<400> 7
gcggaaccca agcuaa                                                 16

 
<210> 8
<211> 4671
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Substrate etc
 
<400> 8
gagttacgcg cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 60
cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 120
cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 180
tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta gcccaccact 240
tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 300
ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 360
aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 420
cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 480
ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 540
agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 600
ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 660
acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 720
cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 780
gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ggcgagagta 840
gggaactgcc aggcatcaaa ctaagcagaa ggcccctgac ggatggcctt tttgcgtttc 900
tacaaactct ttctgtgttg taaaacgacg gccagtctta agctcgggcc ccctgggcgg 960
ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt 1020
tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaagggcg cctgtcactt 1080
tgcttgatat atgagaatta tttaacctta taaatgagaa aaaagcaacg cactttaaat 1140
aagatacgtt gctttttcga ttgatgaaca cctataatta aactattcat ctattattta 1200
tgattttttg tatatacaat atttctagtt tgttaaagag aattaagaaa ataaatctcg 1260
aaaataataa agggaaaatc agtttttgat atcaaaatta tacatgtcaa cgataataca 1320
aaatataata caaactataa gatgttatca gtatttatta tgcatttaga atacgtactc 1380
agcgtctggg ttccccatcg gtgatgtcgt ataagagacg tataggagac ctatagtgtc 1440
ttcggggtaa tacgactcac tatagcggaa cccaagcttg gcattccggt actgttggta 1500
aagccaccat ggaagatgcg aagaacataa agaaaggtcc cgccccattt tacccactcg 1560
aggatggaac agctggggag caactgcaca aggccatgaa gcgctatgcg ttggtgccgg 1620
gaaccatcgc gttcaccgac gcacacatcg aagtgaacat cacttacgcc gagtactttg 1680
agatgagcgt caggctggcc gaggctatga agcgatacgg tctgaacacc aaccaccgga 1740
tcgtggtctg ctctgaaaac agcctgcagt tcttcatgcc ggtcctgggg gccctgttca 1800
tcggcgtggc cgtggcaccc gccaacgata tctacaacga gagagaattg ctgaactcga 1860
tgaacatctc ccagcctacc gtggtgttcg tgtcgaagaa ggggttgcag aagatcctga 1920
acgtgcagaa gaagctgccc atcattcaaa agattatcat tatggattcc aaaaccgact 1980
accagggttt ccagtcaatg tataccttcg tgacctccca tctgccccct ggcttcaacg 2040
aatacgactt cgtgcctgaa agcttcgacc gcgacaagac gatcgccctc atcatgaact 2100
cgtccggctc gaccgggctg cccaaaggag tggccctgcc acaccggacc gcttgcgtgc 2160
ggttctccca cgcccgggac cctattttcg gcaatcagat cattccggac actgccatcc 2220
tgagcgtggt ccccttccat cacgggtttg ggatgtttac cactctgggc tacctcatct 2280
gcggattcag ggtggtgctg atgtaccggt tcgaggaaga acttttcctg cggagcctgc 2340
aggattacaa gatccagtcc gccctcctcg tgccaaccct cttctcattc ttcgctaagt 2400
ccactctcat cgataagtac gacctgtcga atctccacga aattgcgtcc ggtggtgcac 2460
cgctgtccaa ggaggtcggc gaagccgtgg ccaagcgctt ccacctcccg ggaatacgcc 2520
agggatacgg cctgactgaa acgaccagcg cgattctgat caccccggag ggcgacgaca 2580
agccgggtgc cgtggggaaa gtggtgccgt tcttcgaagc aaaggtcgtg gatctggata 2640
ccggaaagac tctgggcgtg aaccagagag gggaactttg tgtgcgcgga ccgatgatta 2700
tgtccggata tgtcaacaac cccgaggcca ctaatgccct gatcgacaag gacggatggt 2760
tgcatagcgg cgacatcgca tactgggacg aggacgagca ctttttcatt gtggatcggc 2820
tcaagtccct gatcaagtac aagggatacc aggtcgcccc tgccgaactt gagtccatcc 2880
tgctgcaaca tccgaacatt ttcgacgcgg gcgtcgctgg ccttcctgat gatgacgccg 2940
gagagctgcc cgcggccgtg gtggtgctcg aacacggaaa aactatgacc gagaaggaaa 3000
tcgtggacta cgtggcgtca caagtcacca ctgccaagaa actgcgcggc ggagtcgtgt 3060
tcgtggacga ggtgcccaag ggcctgaccg gaaagctgga cgctagaaag atccgggaga 3120
tcctgattaa ggccaagaag ggaggaaagt ccaagctctg agatctagag ggccctattc 3180
tatagtgtca cctaaatgct aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 3240
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 3300
gaattcaaaa gaagaccata tacgtctcct ataggtctcg tatactgctt cctatacgac 3360
atcaccgatg gggaacacga agcgatccag ccccccttat tagcccgggc gaaaggccca 3420
gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg 3480
gagtccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag 3540
gtgggaccac cgcgctactg ccgccaggca aacaaggggt gttatgagcc atattcaggt 3600
ataaatgggc tcgcgataat gttcagaatt ggttaattgg ttgtaacact gacccctatt 3660
tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 3720
atgcttcaat aatattgaaa aaggaagaat atgagtattc aacatttccg tgtcgccctt 3780
attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 3840
gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 3900
agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 3960
aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 4020
cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 4080
cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 4140
actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 4200
cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 4260
ataccaaacg acgagcgtga caccacgatg cctgtagcga tggcaacaac gttgcgcaaa 4320
ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 4380
gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 4440
gataaatccg gagccggtga gcgtggttct cgcggtatca tcgcagcgct ggggccagat 4500
ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 4560
cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta agcagagcat 4620
tacgctgact tgacgggacg gcgcaagctc atgaccaaaa tcccttaacg t          4671

 
<210> 9
<211> 1697
<212> RNA
<213> Artificial Sequence
 
<220>
<223> Substrate etc
 
<400> 9
gcggaaccca agcuuggcau uccgguacug uugguaaagc caccauggaa gaugcgaaga 60
acauaaagaa aggucccgcc ccauuuuacc cacucgagga uggaacagcu ggggagcaac 120
ugcacaaggc caugaagcgc uaugcguugg ugccgggaac caucgcguuc accgacgcac 180
acaucgaagu gaacaucacu uacgccgagu acuuugagau gagcgucagg cuggccgagg 240
cuaugaagcg auacggucug aacaccaacc accggaucgu ggucugcucu gaaaacagcc 300
ugcaguucuu caugccgguc cugggggccc uguucaucgg cguggccgug gcacccgcca 360
acgauaucua caacgagaga gaauugcuga acucgaugaa caucucccag ccuaccgugg 420
uguucguguc gaagaagggg uugcagaaga uccugaacgu gcagaagaag cugcccauca 480
uucaaaagau uaucauuaug gauuccaaaa ccgacuacca ggguuuccag ucaauguaua 540
ccuucgugac cucccaucug cccccuggcu ucaacgaaua cgacuucgug ccugaaagcu 600
ucgaccgcga caagacgauc gcccucauca ugaacucguc cggcucgacc gggcugccca 660
aaggaguggc ccugccacac cggaccgcuu gcgugcgguu cucccacgcc cgggacccua 720
uuuucggcaa ucagaucauu ccggacacug ccauccugag cguggucccc uuccaucacg 780
gguuugggau guuuaccacu cugggcuacc ucaucugcgg auucagggug gugcugaugu 840
accgguucga ggaagaacuu uuccugcgga gccugcagga uuacaagauc caguccgccc 900
uccucgugcc aacccucuuc ucauucuucg cuaaguccac ucucaucgau aaguacgacc 960
ugucgaaucu ccacgaaauu gcguccggug gugcaccgcu guccaaggag gucggcgaag 1020
ccguggccaa gcgcuuccac cucccgggaa uacgccaggg auacggccug acugaaacga 1080
ccagcgcgau ucugaucacc ccggagggcg acgacaagcc gggugccgug gggaaagugg 1140
ugccguucuu cgaagcaaag gucguggauc uggauaccgg aaagacucug ggcgugaacc 1200
agagagggga acuuugugug cgcggaccga ugauuauguc cggauauguc aacaaccccg 1260
aggccacuaa ugcccugauc gacaaggacg gaugguugca uagcggcgac aucgcauacu 1320
gggacgagga cgagcacuuu uucauugugg aucggcucaa gucccugauc aaguacaagg 1380
gauaccaggu cgccccugcc gaacuugagu ccauccugcu gcaacauccg aacauuuucg 1440
acgcgggcgu cgcuggccuu ccugaugaug acgccggaga gcugcccgcg gccguggugg 1500
ugcucgaaca cggaaaaacu augaccgaga aggaaaucgu ggacuacgug gcgucacaag 1560
ucaccacugc caagaaacug cgcggcggag ucguguucgu ggacgaggug cccaagggcc 1620
ugaccggaaa gcuggacgcu agaaagaucc gggagauccu gauuaaggcc aagaagggag 1680
gaaaguccaa gcucuga                                                1697

 
<210> 10
<211> 4945
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Substrate etc
 
<400> 10
ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 60
agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 120
cagcgctgcg atgataccgc gagaaccacg ctcaccggct ccggatttat cagcaataaa 180
ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 240
gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 300
cgttgttgcc atcgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 360
cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 420
ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 480
catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 540
tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 600
ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 660
catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 720
cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 780
cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 840
acggaaatgt tgaatactca tattcttcct ttttcaatat tattgaagca tttatcaggg 900
ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 960
cagtgttaca accaattaac caattctgaa cattatcgcg agcccattta tacctgaata 1020
tggctcataa caccccttgt ttgcctggcg gcagtagcgc ggtggtccca cctgacccca 1080
tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag tgtggggact ccccatgcga 1140
gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 1200
cgcccgggct aattatgggg tgtcgccctt cgctgaaggg gtaatacgac tcactatagg 1260
gaaataagag agaaaagaag agtaagaaga aatataagag ccaccatgga agatgcgaag 1320
aacataaaga aaggtcccgc cccattttac ccactcgagg atggaacagc tggggagcaa 1380
ctgcacaagg ccatgaagcg ctatgcgttg gtgccgggaa ccatcgcgtt caccgacgca 1440
cacatcgaag tgaacatcac ttacgccgag tactttgaga tgagcgtcag gctggccgag 1500
gctatgaagc gatacggtct gaacaccaac caccggatcg tggtctgctc tgaaaacagc 1560
ctgcagttct tcatgccggt cctgggggcc ctgttcatcg gcgtggccgt ggcacccgcc 1620
aacgatatct acaacgagag agaattgctg aactcgatga acatctccca gcctaccgtg 1680
gtgttcgtgt cgaagaaggg gttgcagaag atcctgaacg tgcagaagaa gctgcccatc 1740
attcaaaaga ttatcattat ggattccaaa accgactacc agggtttcca gtcaatgtat 1800
accttcgtga cctcccatct gccccctggc ttcaacgaat acgacttcgt gcctgaaagc 1860
ttcgaccgcg acaagacgat cgccctcatc atgaactcgt ccggctcgac cgggctgccc 1920
aaaggagtgg ccctgccaca ccggaccgct tgcgtgcggt tctcccacgc ccgggaccct 1980
attttcggca atcagatcat tccggacact gccatcctga gcgtggtccc cttccatcac 2040
gggtttggga tgtttaccac tctgggctac ctcatctgcg gattcagggt ggtgctgatg 2100
taccggttcg aggaagaact tttcctgcgg agcctgcagg attacaagat ccagtccgcc 2160
ctcctcgtgc caaccctctt ctcattcttc gctaagtcca ctctcatcga taagtacgac 2220
ctgtcgaatc tccacgaaat tgcgtccggt ggtgcaccgc tgtccaagga ggtcggcgaa 2280
gccgtggcca agcgcttcca cctcccggga attcgccagg gatacggcct gactgaaacg 2340
accagcgcga ttctgatcac cccggagggc gacgacaagc cgggtgccgt ggggaaagtg 2400
gtgccgttct tcgaagcaaa ggtcgtggat ctggataccg gaaagactct gggcgtgaac 2460
cagagagggg aactttgtgt gcgcggaccg atgattatgt ccggatatgt caacaacccc 2520
gaggccacta atgccctgat cgacaaggac ggatggttgc atagcggcga catcgcatac 2580
tgggacgagg acgagcactt tttcattgtg gatcggctca agtccctgat caagtacaag 2640
ggataccagg tcgcccctgc cgaacttgag tccatcctgc tgcaacatcc gaacattttc 2700
gacgcgggcg tcgctggcct tcctgatgat gacgccggag agctgcccgc ggccgtggtg 2760
gtgctcgaac acggaaaaac tatgaccgag aaggaaatcg tggactacgt ggcgtcacaa 2820
gtcaccactg ccaagaaact gcgcggcgga gtcgtgttcg tggacgaggt gcccaagggc 2880
ctgaccggaa agctggacgc tagaaagatc cgggagatcc tgattaaggc caagaaggga 2940
ggaaagtcca agctctgaat ctgctgcctt ctgcggggct tgccttctgg ccatgccctt 3000
cttctctccc ttgcacctgt acctcttggt ctttgaataa agcctgagta ggaagtgagg 3060
gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 3120
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagga agagcgctgc 3180
ctccttcaga gtctgctcac gagctcggtc tcggatcccc tatacgacat caccgatggg 3240
gaacacgaag cgatccagcc ccccttatta gcccgggcga aaggcccagt ctttcgactg 3300
agcctttcgt tttatttgat gcctggcagt tccctactct cgcatgggga gtccccacac 3360
taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg 3420
cgctactgcc gccaggcaaa caaggggtgt tatgagccat attcaggtat aaatgggctc 3480
gcgaaaacgt caaaagggcg acacaaaatt tattctaaat gcataataaa tactgataac 3540
atcttatagt ttgtattata ttttgtatta tcgttgacat gtataatttt gatatcaaaa 3600
actgattttc cctttattat tttcgagatt tattttctta attctcttta acaaactaga 3660
aatattgtat atacaaaaaa tcataaataa tagatgaata gtttaattat aggtgttcat 3720
caatcgaaaa agcaacgtat cttatttaaa gtgcgttgct tttttctcat ttataaggtt 3780
aaataattct catatatcaa gcaaagtgac aggcgccctt aaatattctg acaaatgctc 3840
tttccctaaa ctccccccat aaaaaaaccc gccgaagcgg gtttttacgt tatttgcgga 3900
ttaacgatta ctcgttatca gaaccgccca gggggcccga gcttaagact ggccgtcgtt 3960
ttacaacaca gaaagagttt gtagaaacgc aaaaaggcca tccgtcaggg gccttctgct 4020
tagtttgatg cctggcagtt ccctactctc gccttccgct tcctcgctca ctgactcgct 4080
gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 4140
atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 4200
caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 4260
gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 4320
ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 4380
cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 4440
taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 4500
cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 4560
acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 4620
aggcggtgct acagagttct tgaagtggtg ggctaactac ggctacacta gaagaacagt 4680
atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 4740
atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 4800
gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 4860
gtggaacgac gcgcgcgtaa ctcacgttaa gggattttgg tcatgagctt gcgccgtccc 4920
gtcaagtcag cgtaatgctc tgctt                                       4945

 
<210> 11
<211> 1955
<212> RNA
<213> Artificial Sequence
 
<220>
<223> Substrate etc
 
<400> 11
gggaaauaag agagaaaaga agaguaagaa gaaauauaag agccaccaug gaagaugcga 60
agaacauaaa gaaagguccc gccccauuuu acccacucga ggauggaaca gcuggggagc 120
aacugcacaa ggccaugaag cgcuaugcgu uggugccggg aaccaucgcg uucaccgacg 180
cacacaucga agugaacauc acuuacgccg aguacuuuga gaugagcguc aggcuggccg 240
aggcuaugaa gcgauacggu cugaacacca accaccggau cguggucugc ucugaaaaca 300
gccugcaguu cuucaugccg guccuggggg cccuguucau cggcguggcc guggcacccg 360
ccaacgauau cuacaacgag agagaauugc ugaacucgau gaacaucucc cagccuaccg 420
ugguguucgu gucgaagaag ggguugcaga agauccugaa cgugcagaag aagcugccca 480
ucauucaaaa gauuaucauu auggauucca aaaccgacua ccaggguuuc cagucaaugu 540
auaccuucgu gaccucccau cugcccccug gcuucaacga auacgacuuc gugccugaaa 600
gcuucgaccg cgacaagacg aucgcccuca ucaugaacuc guccggcucg accgggcugc 660
ccaaaggagu ggcccugcca caccggaccg cuugcgugcg guucucccac gcccgggacc 720
cuauuuucgg caaucagauc auuccggaca cugccauccu gagcgugguc cccuuccauc 780
acggguuugg gauguuuacc acucugggcu accucaucug cggauucagg guggugcuga 840
uguaccgguu cgaggaagaa cuuuuccugc ggagccugca ggauuacaag auccaguccg 900
cccuccucgu gccaacccuc uucucauucu ucgcuaaguc cacucucauc gauaaguacg 960
accugucgaa ucuccacgaa auugcguccg guggugcacc gcuguccaag gaggucggcg 1020
aagccguggc caagcgcuuc caccucccgg gaauucgcca gggauacggc cugacugaaa 1080
cgaccagcgc gauucugauc accccggagg gcgacgacaa gccgggugcc guggggaaag 1140
uggugccguu cuucgaagca aaggucgugg aucuggauac cggaaagacu cugggcguga 1200
accagagagg ggaacuuugu gugcgcggac cgaugauuau guccggauau gucaacaacc 1260
ccgaggccac uaaugcccug aucgacaagg acggaugguu gcauagcggc gacaucgcau 1320
acugggacga ggacgagcac uuuuucauug uggaucggcu caagucccug aucaaguaca 1380
agggauacca ggucgccccu gccgaacuug aguccauccu gcugcaacau ccgaacauuu 1440
ucgacgcggg cgucgcuggc cuuccugaug augacgccgg agagcugccc gcggccgugg 1500
uggugcucga acacggaaaa acuaugaccg agaaggaaau cguggacuac guggcgucac 1560
aagucaccac ugccaagaaa cugcgcggcg gagucguguu cguggacgag gugcccaagg 1620
gccugaccgg aaagcuggac gcuagaaaga uccgggagau ccugauuaag gccaagaagg 1680
gaggaaaguc caagcucuga aucugcugcc uucugcgggg cuugccuucu ggccaugccc 1740
uucuucucuc ccuugcaccu guaccucuug gucuuugaau aaagccugag uaggaaguga 1800
gggaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 1860
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaag gaagagcgcu 1920
gccuccuuca gagucugcuc acgagcucgg ucucg                            1955

 
<210> 12
<211> 57
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Substrate etc
 
<400> 12
tctagaggcc agcctggcca taaggagata tacatcggta ctgttggtaa agccacc    57

 
<210> 13
<211> 50
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Substrate etc
 
<400> 13
taatgaggcc aaactggcca ccatcaccat cagagcttgg actttcctcc            50


<210> 14
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 14
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct 1200
cgccgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaagatt ctccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat 1980
accattcagt gggctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 15
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 15
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Arg Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Asp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Asp Thr Ile Gln Trp Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 16
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 16
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgctga taatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct 1200
agacgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaagatt ctccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gctgggtgac aaagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaaacc 1980
accattcagt gggctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 17
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 17
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Arg Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Asp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Trp Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Thr Thr Ile Gln Trp Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 18
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 18
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgctga taatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct 1200
cgccgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaattgt ctccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaatgg 1980
accattcagt gggctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 19
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 19
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Arg Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Leu Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Trp Thr Ile Gln Trp Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 20
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 20
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgcttg gaatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct 1200
agtcgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaacggt ctccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat 1980
accattcagt gggctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 21
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 21
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Trp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Ser Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Arg Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Asp Thr Ile Gln Trp Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 22
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 22
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgcttg gaatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct 1200
agacgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaatggt ctccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat 1980
accattcagt gggctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 23
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 23
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Trp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Arg Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Trp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Asp Thr Ile Gln Trp Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 24
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 24
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgcttg gaatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct 1200
agtcgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaatggt ctccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat 1980
accattcagt gggctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 25
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 25
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Trp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Ser Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Trp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Asp Thr Ile Gln Trp Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 26
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 26
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgcttg gaatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct 1200
atacgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaagatt ctccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat 1980
accattcagt gggctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 27
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 27
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Trp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Ile Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Asp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Asp Thr Ile Gln Trp Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 28
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 28
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtataga caatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga agacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca gagacaaggc tcgcaagtct 1200
cgccgtatct atcttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgattggac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaagatt ccccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat 1980
accattcagt gggctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 29
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 29
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ile Asp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Arg Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Arg Arg Ile Tyr Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Trp Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Asp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Asp Thr Ile Gln Trp Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 30
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 30
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc gcgcaagtct 1200
cgccgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaatggt ctccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gctgggtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaattc 1980
accattcagc ctgctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 31
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 31
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Arg Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Trp Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Trp Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Phe Thr Ile Gln Pro Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 32
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 32
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc gcgcaagtct 1200
cgccgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaatatt ctccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gctgggtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaatac 1980
accattcagc ctgctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 33
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 33
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Arg Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Tyr Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Trp Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Tyr Thr Ile Gln Pro Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 34
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 34
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgcgga caatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc tcgcaagtct 1200
gttcgtatct atcttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaagatc tcccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagac 1980
accattcagt gggctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 35
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 35
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Val Arg Ile Tyr Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Asp Leu Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Asp Thr Ile Gln Trp Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 36
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 36
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgcgga caatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtccg ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca gagacaaggc tcgcaagtct 1200
gttcgtatct atcttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaagatc tcccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gcagtgtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaagat 1980
actattcagt gggctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 37
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 37
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Arg Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Arg Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Val Arg Ile Tyr Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Asp Leu Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Ser Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Asp Thr Ile Gln Trp Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         

 
<210> 38
<211> 2673
<212> DNA
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 38
atgcaccatc accatcacca tatgaacacg attaacatcg ctaagaacga cttctctgac 60
atcgaactgg ctgctatccc gttcaacact ctggctgacc attacggtga gcgtttagct 120
cgcgaacagt tggcccttga gcatgagtct tacgagatgg gtgaagcacg cttccgcaag 180
atgtttgagc gtcaacttaa agctggtgag gttgcggata acgctgccgc caagcctctc 240
atcactaccc tactccctaa gatgattgca cgcatcaacg actggtttga ggaagtgaaa 300
gctaagcgcg gcaagcgccc gacagccttc cagttcctgc aagaaatcaa gccggaagcc 360
gtagcgtaca tcaccattaa gaccactctg gcttgcctaa ccagtgctga caatacaacc 420
gttcaggctg tagcaagcgc aatcggtcgg gccattgagg acgaggctcg cttcggtcgt 480
atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg aggaacaact caacaagcgc 540
gtagggcacg tctacaagaa agcatttatg caagttgtcg aggctgacat gctctctaag 600
ggtctactcg gtggcgaggc gtggtcttcg tggcataagg aagactctat tcatgtagga 660
gtacgctgca tcgagatgct cattgagtca accggaatgg ttagcttaca ccgccaaaat 720
gctggcgtag taggtcaaga ctctgagact atcgaactcg cacctgaata cgctgaggct 780
atcgcaaccc gtgcaggtgc gctggctggc atctctccga tgttccaacc ttgcgtagtt 840
cctcctaagc cgtggactgg cattactggt ggtggctatt gggctaacgg tcgtcgtcct 900
ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc gctacgaaga cgtttacatg 960
cctgaggtgt acaaagcgat taacattgcg caaaacaccg catggaaaat caacaagaaa 1020
gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt gtccggtcga ggacatccct 1080
gcgattgagc gtgaagaact cccgatgaaa ccggaagaca tcgacatgaa tcctgaggct 1140
ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca aggacaaggc gcgcaagtct 1200
cgccgtatca gccttgagtt catgcttgag caagccaata agtttgctaa ccataaggcc 1260
atctggttcc cttacaacat ggactggcgc ggtcgtgttt acgctgtgtc aatgttcaac 1320
ccgcaaggta acgatatgac caaaggactg cttacgctgg cgaaaggtaa accaatcggt 1380
aaggaaggtt actactggct gaaaatccac ggtgcaaact gtgcgggtgt cgataaggtt 1440
ccgttccctg agcgcatcaa gttcattgag gaaaaccacg agaacatcat ggcttgcgct 1500
aagtctccac tggagaacac ttggtgggct gagcaatatt ctccgttctg cttccttgcg 1560
ttctgctttg agtacgctgg ggtacagcac cacggcctga gctataactg ctcccttccg 1620
ctggcgtttg acgggtcttg ctctggcatc cagcacttct ccgcgatgct ccgagatgag 1680
gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg ttcaggacat ctacgggatt 1740
gttgctaaga aagtcaacga gattctacaa gcagacgcaa tcaatgggac cgataacgaa 1800
gtagttaccg tgaccgatga gaacactggt gaaatctctg agaaagtcaa gctgggcact 1860
aaggcactgg ctggtcaatg gctggcttac ggtgttactc gctgggtgac taagcgttca 1920
gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc gtcaacaagt gctggaattc 1980
accattcagc ctgctattga ttccggcaag ggtctgatgt tcactcagcc gaatcaggct 2040
gctggataca tggctaagct gatttgggaa tctgtgagcg tgacggtggt agctgcggtt 2100
gaagcaatga actggcttaa gtctgctgct aagctgctgg ctgctgaggt caaagataag 2160
aagactggag agattcttcg caagcgttgc gctgtgcatt gggtaactcc tgatggtttc 2220
cctgtgtggc aggaatacaa gaagcctatt cagacgcgct tgaacctgat gttcctcggt 2280
cagttccgct tacagcctac cattaacacc aacaaagata gcgagattga tgcacacaaa 2340
caggagtctg gtatcgctcc taactttgta cacagccaag acggtagcca ccttcgtaag 2400
actgtagtgt gggcacacga gaagtacgga atcgaatctt ttgcactgat tcacgactcc 2460
ttcggtacca ttccggctga cgctgcgaac ctgttcaaag cagtgcgcga aactatggtt 2520
gacacatatg agtcttgtga tgtactggct gatttctacg accagttcgc tgaccagttg 2580
cacgagtctc aattggacaa aatgccagca cttccggcta aaggtaactt gaacctccgt 2640
gacatcttag agtcggactt cgcgttcgcg taa                              2673

 
<210> 39
<211> 890
<212> PRT
<213> Artificial Sequence
 
<220>
<223> T7RNAP variant
 
<400> 39
Met His His His His His His Met Asn Thr Ile Asn Ile Ala Lys Asn 
1               5                   10                  15      
Asp Phe Ser Asp Ile Glu Leu Ala Ala Ile Pro Phe Asn Thr Leu Ala 
            20                  25                  30          
Asp His Tyr Gly Glu Arg Leu Ala Arg Glu Gln Leu Ala Leu Glu His 
        35                  40                  45              
Glu Ser Tyr Glu Met Gly Glu Ala Arg Phe Arg Lys Met Phe Glu Arg 
    50                  55                  60                  
Gln Leu Lys Ala Gly Glu Val Ala Asp Asn Ala Ala Ala Lys Pro Leu 
65                  70                  75                  80  
Ile Thr Thr Leu Leu Pro Lys Met Ile Ala Arg Ile Asn Asp Trp Phe 
                85                  90                  95      
Glu Glu Val Lys Ala Lys Arg Gly Lys Arg Pro Thr Ala Phe Gln Phe 
            100                 105                 110         
Leu Gln Glu Ile Lys Pro Glu Ala Val Ala Tyr Ile Thr Ile Lys Thr 
        115                 120                 125             
Thr Leu Ala Cys Leu Thr Ser Ala Asp Asn Thr Thr Val Gln Ala Val 
    130                 135                 140                 
Ala Ser Ala Ile Gly Arg Ala Ile Glu Asp Glu Ala Arg Phe Gly Arg 
145                 150                 155                 160 
Ile Arg Asp Leu Glu Ala Lys His Phe Lys Lys Asn Val Glu Glu Gln 
                165                 170                 175     
Leu Asn Lys Arg Val Gly His Val Tyr Lys Lys Ala Phe Met Gln Val 
            180                 185                 190         
Val Glu Ala Asp Met Leu Ser Lys Gly Leu Leu Gly Gly Glu Ala Trp 
        195                 200                 205             
Ser Ser Trp His Lys Glu Asp Ser Ile His Val Gly Val Arg Cys Ile 
    210                 215                 220                 
Glu Met Leu Ile Glu Ser Thr Gly Met Val Ser Leu His Arg Gln Asn 
225                 230                 235                 240 
Ala Gly Val Val Gly Gln Asp Ser Glu Thr Ile Glu Leu Ala Pro Glu 
                245                 250                 255     
Tyr Ala Glu Ala Ile Ala Thr Arg Ala Gly Ala Leu Ala Gly Ile Ser 
            260                 265                 270         
Pro Met Phe Gln Pro Cys Val Val Pro Pro Lys Pro Trp Thr Gly Ile 
        275                 280                 285             
Thr Gly Gly Gly Tyr Trp Ala Asn Gly Arg Arg Pro Leu Ala Leu Val 
    290                 295                 300                 
Arg Thr His Ser Lys Lys Ala Leu Met Arg Tyr Glu Asp Val Tyr Met 
305                 310                 315                 320 
Pro Glu Val Tyr Lys Ala Ile Asn Ile Ala Gln Asn Thr Ala Trp Lys 
                325                 330                 335     
Ile Asn Lys Lys Val Leu Ala Val Ala Asn Val Ile Thr Lys Trp Lys 
            340                 345                 350         
His Cys Pro Val Glu Asp Ile Pro Ala Ile Glu Arg Glu Glu Leu Pro 
        355                 360                 365             
Met Lys Pro Glu Asp Ile Asp Met Asn Pro Glu Ala Leu Thr Ala Trp 
    370                 375                 380                 
Lys Arg Ala Ala Ala Ala Val Tyr Arg Lys Asp Lys Ala Arg Lys Ser 
385                 390                 395                 400 
Arg Arg Ile Ser Leu Glu Phe Met Leu Glu Gln Ala Asn Lys Phe Ala 
                405                 410                 415     
Asn His Lys Ala Ile Trp Phe Pro Tyr Asn Met Asp Trp Arg Gly Arg 
            420                 425                 430         
Val Tyr Ala Val Ser Met Phe Asn Pro Gln Gly Asn Asp Met Thr Lys 
        435                 440                 445             
Gly Leu Leu Thr Leu Ala Lys Gly Lys Pro Ile Gly Lys Glu Gly Tyr 
    450                 455                 460                 
Tyr Trp Leu Lys Ile His Gly Ala Asn Cys Ala Gly Val Asp Lys Val 
465                 470                 475                 480 
Pro Phe Pro Glu Arg Ile Lys Phe Ile Glu Glu Asn His Glu Asn Ile 
                485                 490                 495     
Met Ala Cys Ala Lys Ser Pro Leu Glu Asn Thr Trp Trp Ala Glu Gln 
            500                 505                 510         
Tyr Ser Pro Phe Cys Phe Leu Ala Phe Cys Phe Glu Tyr Ala Gly Val 
        515                 520                 525             
Gln His His Gly Leu Ser Tyr Asn Cys Ser Leu Pro Leu Ala Phe Asp 
    530                 535                 540                 
Gly Ser Cys Ser Gly Ile Gln His Phe Ser Ala Met Leu Arg Asp Glu 
545                 550                 555                 560 
Val Gly Gly Arg Ala Val Asn Leu Leu Pro Ser Glu Thr Val Gln Asp 
                565                 570                 575     
Ile Tyr Gly Ile Val Ala Lys Lys Val Asn Glu Ile Leu Gln Ala Asp 
            580                 585                 590         
Ala Ile Asn Gly Thr Asp Asn Glu Val Val Thr Val Thr Asp Glu Asn 
        595                 600                 605             
Thr Gly Glu Ile Ser Glu Lys Val Lys Leu Gly Thr Lys Ala Leu Ala 
    610                 615                 620                 
Gly Gln Trp Leu Ala Tyr Gly Val Thr Arg Trp Val Thr Lys Arg Ser 
625                 630                 635                 640 
Val Met Thr Leu Ala Tyr Gly Ser Lys Glu Phe Gly Phe Arg Gln Gln 
                645                 650                 655     
Val Leu Glu Phe Thr Ile Gln Pro Ala Ile Asp Ser Gly Lys Gly Leu 
            660                 665                 670         
Met Phe Thr Gln Pro Asn Gln Ala Ala Gly Tyr Met Ala Lys Leu Ile 
        675                 680                 685             
Trp Glu Ser Val Ser Val Thr Val Val Ala Ala Val Glu Ala Met Asn 
    690                 695                 700                 
Trp Leu Lys Ser Ala Ala Lys Leu Leu Ala Ala Glu Val Lys Asp Lys 
705                 710                 715                 720 
Lys Thr Gly Glu Ile Leu Arg Lys Arg Cys Ala Val His Trp Val Thr 
                725                 730                 735     
Pro Asp Gly Phe Pro Val Trp Gln Glu Tyr Lys Lys Pro Ile Gln Thr 
            740                 745                 750         
Arg Leu Asn Leu Met Phe Leu Gly Gln Phe Arg Leu Gln Pro Thr Ile 
        755                 760                 765             
Asn Thr Asn Lys Asp Ser Glu Ile Asp Ala His Lys Gln Glu Ser Gly 
    770                 775                 780                 
Ile Ala Pro Asn Phe Val His Ser Gln Asp Gly Ser His Leu Arg Lys 
785                 790                 795                 800 
Thr Val Val Trp Ala His Glu Lys Tyr Gly Ile Glu Ser Phe Ala Leu 
                805                 810                 815     
Ile His Asp Ser Phe Gly Thr Ile Pro Ala Asp Ala Ala Asn Leu Phe 
            820                 825                 830         
Lys Ala Val Arg Glu Thr Met Val Asp Thr Tyr Glu Ser Cys Asp Val 
        835                 840                 845             
Leu Ala Asp Phe Tyr Asp Gln Phe Ala Asp Gln Leu His Glu Ser Gln 
    850                 855                 860                 
Leu Asp Lys Met Pro Ala Leu Pro Ala Lys Gly Asn Leu Asn Leu Arg 
865                 870                 875                 880 
Asp Ile Leu Glu Ser Asp Phe Ala Phe Ala                         
                885                 890                         


<210> 40
<211> 25
<212> DNA
<213> Artificial Sequence
 
<220>
<223> Substrate etc
 
<400> 40
tttttttttt tttttttttt ttttt                                       25

