                         SEQUENCE LISTING

<110>  COMPAGNIE GENERALE DES ETABLISSEMENTS MICHELIN
 
<120>  Utilisation des polyktide synthases de type III comme 
       phloroglucinol synthases

<130>  B374764PCTD37151

<150>  FR1756108
<151>  2017-06-30

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  413
<212>  PRT
<213>  Sargassum binderi

<400>  1

Met Ser Ser Ala Ala Val Ala Met Leu Ala Asp Pro Thr Val Gln Ile 
1               5                   10                  15      


Ala Leu Ala Cys Ile Val Leu Ser Leu Ile Val Val Phe Arg Ser Tyr 
            20                  25                  30          


Arg Lys Gly Lys Asp Glu Gln Thr Val Tyr Pro Val Ile Ala Gly Met 
        35                  40                  45              


Ala Ile Gly Asn Pro Gln Tyr Arg Cys Thr Gln Asp Gln Ala Leu Thr 
    50                  55                  60                  


Val Ala Gln Lys Cys Pro Gly Val Glu Ser Val Lys Pro Val Leu Glu 
65                  70                  75                  80  


Arg Ile Tyr Gly Asn Ser Arg Ile Gly Ser Arg Tyr Phe Ala Val Pro 
                85                  90                  95      


Asp Phe Thr Pro Asn Gln Ala Ala Lys Gly Asp Pro Met Phe Phe Pro 
            100                 105                 110         


Ala Asp Gly Ser Phe Glu Val Pro Val Asp Thr Arg Leu Asp Lys Phe 
        115                 120                 125             


Lys Glu Lys Ala Val Pro Leu Val Ser Asp Val Ala Arg Arg Ala Ile 
    130                 135                 140                 


Lys Glu Ala Gly Ile Asp Val Ser Asp Val Ser Lys Leu Val Val Val 
145                 150                 155                 160 


Ser Ser Thr Gly Phe Leu Gly Pro Gly Leu Asp Cys Glu Leu Ile Lys 
                165                 170                 175     


Asn Leu Gly Leu Thr Arg Ser Val Asp Arg Thr Leu Ile Gly Phe Met 
            180                 185                 190         


Gly Cys Ala Ala Ala Met Asn Gly Phe Arg Asn Ala Asn Asp Phe Val 
        195                 200                 205             


Thr Ala Asn Pro Gly Lys Tyr Ala Leu Met Ile Cys Val Glu Leu Ser 
    210                 215                 220                 


Ser Val His Thr Thr Phe Asp Asp Asn Ile Asn Asp Ala Ile Leu His 
225                 230                 235                 240 


Ala Ile Phe Ala Asp Gly Cys Ala Ala Ala Val Leu Lys Gly Val Arg 
                245                 250                 255     


Lys Glu Ala Pro Lys Gly Thr Leu Ala Ile Val Asp Asn His Ala Trp 
            260                 265                 270         


Leu Met Glu Gly Thr Glu Asp Gly Ile Thr Leu Ala Ile Lys Pro Asn 
        275                 280                 285             


Gly Ile Thr Cys Thr Leu Ser Lys Phe Leu Pro Gln Tyr Ile Ala Lys 
    290                 295                 300                 


Asn Ile Ala Phe Phe Ala Asp Gly Phe Leu Lys Lys His Asn Leu Gly 
305                 310                 315                 320 


Arg Asp Asp Val Asp Phe Trp Cys Val His Pro Gly Gly Arg Arg Ile 
                325                 330                 335     


Ile Glu Glu Ala Gln Asn Gly Leu Gly Leu Thr Glu Ala Gln Thr Ala 
            340                 345                 350         


Asp Ser Trp Ala Val Leu Ala Glu Tyr Gly Asn Met Leu Ser Pro Ser 
        355                 360                 365             


Val Met Phe Val Leu Ser Arg Val Phe Lys Arg His Asn Ala Ala Leu 
    370                 375                 380                 


Ala Gln Gly Lys Pro Gly Tyr Gln Thr Gly Met Ala Phe Ser Phe Ser 
385                 390                 395                 400 


Pro Gly Val Gly Ala Glu Gly Ile Leu Leu Arg Gln Ile 
                405                 410             


<210>  2
<211>  297
<212>  PRT
<213>  Aureococcus anophagefferens

<400>  2

Met Pro Val Glu Lys Arg Leu Asp Met Phe Arg Glu Lys Ser Val Pro 
1               5                   10                  15      


Leu Val Thr Lys Val Cys Lys Asp Ala Met Ala Asp Ala Gly Ile Asp 
            20                  25                  30          


Val Glu Gln Ile Gly Lys Leu Val Val Val Ser Ser Thr Gly Phe Leu 
        35                  40                  45              


Gly Pro Gly Leu Asp Ala Glu Leu Ile Lys Thr Leu Gly Leu Trp Arg 
    50                  55                  60                  


Gly Val Asp Arg Ser Leu Ile Gly Phe Met Gly Cys Ala Ala Ala Met 
65                  70                  75                  80  


Asn Gly Phe Arg Val Ala Asn Asp Phe Ala Met Ser His Pro Gly Lys 
                85                  90                  95      


Met Ala Leu Met Val Cys Val Glu Ile Ser Ser Val His Thr Thr Phe 
            100                 105                 110         


Asp Asp Asn Val Asn Asp Ala Ile Leu His Ala Ile Phe Ala Asp Gly 
        115                 120                 125             


Cys Ala Ala Ala Val Ile Ser Gly Glu Lys Pro Gly Ser Ala Ala Ala 
    130                 135                 140                 


Lys Gly Lys Phe Gly Ile Val Asp Thr His Gly Trp Leu Met Glu Gly 
145                 150                 155                 160 


Thr Glu Asp Gly Ile Thr Leu Ser Ile Asn Glu Asn Gly Ile Ser Cys 
                165                 170                 175     


Ile Leu Ser Lys Tyr Leu Pro Gln Tyr Ile Ala Lys Asn Met Ala Gly 
            180                 185                 190         


Tyr Val Asp Ser Phe Leu Gly Met His Gly Leu Gln Lys Thr Asp Met 
        195                 200                 205             


Asp Phe Trp Ala Ile His Pro Gly Gly Arg Arg Ile Ile Glu Glu Ala 
    210                 215                 220                 


Gln Asn Gly Leu Gly Leu Ser Glu Glu Gln Ala Lys Tyr Ser Trp Thr 
225                 230                 235                 240 


Val Leu Ser Gln Tyr Gly Asn Met Leu Ser Pro Ser Val Met Phe Val 
                245                 250                 255     


Leu Glu Leu Ile Leu Asn Asp His Lys Lys Ala Leu Ala Lys Gly Glu 
            260                 265                 270         


Arg Gly Leu Lys Gln Gly Ile Ala Phe Ser Phe Ser Pro Gly Val Gly 
        275                 280                 285             


Ala Glu Gly Ile Leu Ile Asn Val Met 
    290                 295         


<210>  3
<211>  382
<212>  PRT
<213>  Aureococcus anophagefferens

<400>  3

Met Ser Lys Lys Asp Glu Lys Ile Ile Pro Val Ile Met Gly Met Ala 
1               5                   10                  15      


Thr Gly Asn Pro Pro Tyr Arg Ala Ser Gln Gln Gln Ala Leu Ala Ile 
            20                  25                  30          


Ala Glu Ser Cys Pro Glu Cys Asn Ser Ile Lys Pro Val Leu Ala Arg 
        35                  40                  45              


Ile Tyr Gly Asn Ser Arg Ile Asp Tyr Arg Phe Met Ala Val Pro Asp 
    50                  55                  60                  


Phe Thr Pro Glu Gln Lys Leu Glu Gly Asp Glu Asn Phe Phe Asp Lys 
65                  70                  75                  80  


Asp Leu Met Phe Lys Met Pro Val Glu Lys Arg Leu Asp Met Phe Arg 
                85                  90                  95      


Glu Lys Ser Val Pro Leu Val Thr Lys Val Cys Lys Asp Ala Met Ala 
            100                 105                 110         


Asp Ala Gly Ile Asp Val Glu Gln Ile Gly Lys Leu Val Val Val Ser 
        115                 120                 125             


Ser Thr Gly Phe Leu Gly Pro Gly Leu Asp Ala Glu Leu Ile Lys Thr 
    130                 135                 140                 


Leu Gly Leu Trp Arg Gly Val Asp Arg Ser Leu Ile Gly Phe Met Gly 
145                 150                 155                 160 


Cys Ala Ala Ala Met Asn Gly Phe Arg Val Ala Asn Asp Phe Ala Met 
                165                 170                 175     


Ser His Pro Gly Lys Met Ala Leu Met Val Cys Val Glu Ile Ser Ser 
            180                 185                 190         


Val His Thr Thr Phe Asp Asp Asn Val Asn Asp Ala Ile Leu His Ala 
        195                 200                 205             


Ile Phe Ala Asp Gly Cys Ala Ala Ala Val Ile Ser Gly Glu Lys Pro 
    210                 215                 220                 


Gly Ser Ala Ala Ala Lys Gly Lys Phe Gly Ile Val Asp Thr His Gly 
225                 230                 235                 240 


Trp Leu Met Glu Gly Thr Glu Asp Gly Ile Thr Leu Ser Ile Asn Glu 
                245                 250                 255     


Asn Gly Ile Ser Cys Ile Leu Ser Lys Tyr Leu Pro Gln Tyr Ile Ala 
            260                 265                 270         


Lys Asn Met Ala Gly Tyr Val Asp Ser Phe Leu Gly Met His Gly Leu 
        275                 280                 285             


Gln Lys Thr Asp Met Asp Phe Trp Ala Ile His Pro Gly Gly Arg Arg 
    290                 295                 300                 


Ile Ile Glu Glu Ala Gln Asn Gly Leu Gly Leu Ser Glu Glu Gln Ala 
305                 310                 315                 320 


Lys Tyr Ser Trp Thr Val Leu Ser Gln Tyr Gly Asn Met Leu Ser Pro 
                325                 330                 335     


Ser Val Met Phe Val Leu Glu Leu Ile Leu Asn Asp His Lys Lys Ala 
            340                 345                 350         


Leu Ala Lys Gly Glu Arg Gly Leu Lys Gln Gly Ile Ala Phe Ser Phe 
        355                 360                 365             


Ser Pro Gly Val Gly Ala Glu Gly Ile Leu Ile Asn Val Met 
    370                 375                 380         


<210>  4
<211>  1149
<212>  DNA
<213>  Aureococcus anophagefferens

<400>  4
atgtcgaaga aggatgagaa gatcatccct gtcatcatgg gtatggccac aggtaatccg       60

ccatacaggg cgagtcaaca acaagctcta gcaattgcgg agtcctgccc agaatgtaat      120

agcattaaac cggtattggc aagaatttat ggtaatagcc gtatcgacta caggtttatg      180

gcagtaccgg atttcacacc cgaacagaag cttgaaggtg acgaaaactt ctttgataaa      240

gatttaatgt ttaagatgcc agtggagaag agattggaca tgtttaggga gaagagtgtg      300

ccattagtca cgaaggtctg taaggatgcc atggcagacg ctggtataga cgttgaacag      360

attggcaaac ttgtcgtggt atcatcaact ggcttcttag gccctggact agatgcggag      420

ttaattaaga cacttggact ttggcgtggt gttgaccggt ctctgattgg attcatgggt      480

tgtgcggcgg ccatgaacgg ttttcgggtt gctaatgatt tcgccatgtc ccacccggga      540

aagatggccc taatggtttg cgtcgaaata tcctctgtgc ataccacatt cgatgataac      600

gtaaatgatg caatacttca tgcgatcttc gccgatggat gtgcagccgc agtaatatcg      660

ggagagaagc caggttctgc ggcagcgaaa ggcaaattcg gaatcgtcga tacgcatggt      720

tggctcatgg aaggcacaga agatgggata accctgtcta tcaatgaaaa tggtatatca      780

tgcatcttga gtaagtatct accacagtat attgcaaaga acatggcagg ttacgtagat      840

agtttcctag ggatgcatgg attacagaag acagatatgg atttctgggc tattcacccc      900

ggcggccgca gaattataga ggaagcgcag aacggtttgg gtttatccga ggagcaggcg      960

aagtattctt ggactgttct tagtcagtat ggtaatatgt tgtcacctag cgtgatgttt     1020

gtgttagaac tgatccttaa tgaccacaag aaggcactgg caaagggaga gcgcggttta     1080

aagcaaggta tcgcatttag cttctcaccg ggtgttgggg ccgagggcat cctcattaat     1140

gttatgtaa                                                             1149


<210>  5
<211>  349
<212>  PRT
<213>  Pseudomonas fluorescens Pf5

<400>  5

Met Ser Thr Leu Cys Leu Pro His Val Met Phe Pro Gln His Lys Ile 
1               5                   10                  15      


Thr Gln Gln Gln Met Val Asp His Leu Glu Asn Leu His Ala Asp His 
            20                  25                  30          


Pro Arg Met Ala Leu Ala Lys Arg Met Ile Ala Asn Thr Glu Val Asn 
        35                  40                  45              


Glu Arg His Leu Val Leu Pro Ile Asp Glu Leu Ala Val His Thr Gly 
    50                  55                  60                  


Phe Thr His Arg Ser Ile Val Tyr Glu Arg Glu Ala Arg Gln Met Ser 
65                  70                  75                  80  


Ser Ala Ala Ala Arg Gln Ala Ile Glu Asn Ala Gly Leu Gln Ile Ser 
                85                  90                  95      


Asp Ile Arg Met Val Ile Val Thr Ser Cys Thr Gly Phe Met Met Pro 
            100                 105                 110         


Ser Leu Thr Ala His Leu Ile Asn Asp Leu Ala Leu Pro Thr Ser Thr 
        115                 120                 125             


Val Gln Leu Pro Ile Ala Gln Leu Gly Cys Val Ala Gly Ala Ala Ala 
    130                 135                 140                 


Ile Asn Arg Ala Asn Asp Phe Ala Arg Leu Asp Ala Arg Asn His Val 
145                 150                 155                 160 


Leu Ile Val Ser Leu Glu Phe Ser Ser Leu Cys Tyr Gln Pro Asp Asp 
                165                 170                 175     


Thr Lys Leu His Ala Phe Ile Ser Ala Ala Leu Phe Gly Asp Ala Val 
            180                 185                 190         


Ser Ala Cys Val Leu Arg Ala Asp Asp Gln Ala Gly Gly Phe Lys Ile 
        195                 200                 205             


Lys Lys Thr Glu Ser Tyr Phe Leu Pro Lys Ser Glu His Tyr Ile Lys 
    210                 215                 220                 


Tyr Asp Val Lys Asp Thr Gly Phe His Phe Thr Leu Asp Lys Ala Val 
225                 230                 235                 240 


Met Asn Ser Ile Lys Asp Val Ala Pro Val Met Glu Arg Leu Asn Tyr 
                245                 250                 255     


Glu Ser Phe Glu Gln Asn Cys Ala His Asn Asp Phe Phe Ile Phe His 
            260                 265                 270         


Thr Gly Gly Arg Lys Ile Leu Asp Glu Leu Val Met His Leu Asp Leu 
        275                 280                 285             


Ala Ser Asn Arg Val Ser Gln Ser Arg Ser Ser Leu Ser Glu Ala Gly 
    290                 295                 300                 


Asn Ile Ala Ser Val Val Val Phe Asp Val Leu Lys Arg Gln Phe Asp 
305                 310                 315                 320 


Ser Asn Leu Asn Arg Gly Asp Ile Gly Leu Leu Ala Ala Phe Gly Pro 
                325                 330                 335     


Gly Phe Thr Ala Glu Met Ala Val Gly Glu Trp Thr Ala 
            340                 345                 


<210>  6
<211>  381
<212>  PRT
<213>  Ectocarpus siliculosus

<400>  6

Met Ser Lys Asp Glu Gln Thr Val Tyr Pro Val Ile Ala Gly Met Ala 
1               5                   10                  15      


Ile Gly Asn Pro Gln Tyr Arg Cys Thr Gln Asn Glu Ala Leu Ala Val 
            20                  25                  30          


Ala Ser Lys Cys Pro Gly Leu Glu Ser Ile Lys Pro Val Leu Glu Arg 
        35                  40                  45              


Ile Tyr Gly Asn Ser Arg Ile Gly Ser Arg Tyr Phe Ala Val Pro Asp 
    50                  55                  60                  


Phe Thr Pro Gly Arg Ala Ala Lys Gly Asp Pro Leu Phe Tyr Pro Ala 
65                  70                  75                  80  


Asp Gly Ser Tyr Gln Val Pro Val Asp Val Arg Leu Asp Lys Phe Lys 
                85                  90                  95      


Glu Lys Ala Val Pro Leu Val Ser Asp Val Ala Arg Arg Ala Ile Lys 
            100                 105                 110         


Glu Ala Gly Leu Asn Val Glu Asp Ile Ser Lys Leu Val Val Val Ser 
        115                 120                 125             


Ser Thr Gly Phe Leu Gly Pro Gly Leu Asp Cys Glu Leu Ile Lys Asn 
    130                 135                 140                 


Leu Gly Leu Thr Arg Ser Val Asp Arg Thr Leu Ile Gly Phe Met Gly 
145                 150                 155                 160 


Cys Ala Ala Ala Met Asn Gly Phe Arg Asn Ala Asn Asp Tyr Val Thr 
                165                 170                 175     


Ala Asn Pro Gly Lys Tyr Ala Leu Met Ile Cys Val Glu Leu Ser Ser 
            180                 185                 190         


Val His Thr Thr Phe Asp Asp Asn Ile Asn Asp Ala Ile Leu His Ala 
        195                 200                 205             


Ile Phe Ala Asp Gly Cys Ala Ala Ala Val Leu Lys Gly Ala Arg Lys 
    210                 215                 220                 


Ser Glu Cys Pro Lys Gly Thr Leu Ala Ile Val Asp Asn His Ala Trp 
225                 230                 235                 240 


Leu Met Glu Gly Thr Glu Asp Gly Ile Thr Leu Ala Ile Lys Pro Asn 
                245                 250                 255     


Gly Ile Thr Cys Thr Leu Ser Lys Phe Leu Pro Gln Tyr Ile Ala Lys 
            260                 265                 270         


Asn Ile Ala Phe Phe Ala Asp Gly Phe Leu Lys Lys His Lys Leu Gly 
        275                 280                 285             


Arg Asp Asp Val Asp Phe Trp Cys Val His Pro Gly Gly Arg Arg Ile 
    290                 295                 300                 


Ile Glu Glu Ala Gln Asn Gly Leu Gly Leu Ser Glu Glu Gln Thr Ala 
305                 310                 315                 320 


Asp Ser Trp Ala Val Leu Gly Glu Tyr Gly Asn Met Leu Ser Pro Ser 
                325                 330                 335     


Val Met Phe Val Leu Ser Arg Val Phe Lys Arg His Asn Ala Ala Leu 
            340                 345                 350         


Ala Gln Gly Lys Pro Gly Tyr Gln Thr Gly Met Ala Phe Ser Phe Ser 
        355                 360                 365             


Pro Gly Val Gly Ala Glu Gly Ile Leu Leu Arg Gln Ile 
    370                 375                 380     


<210>  7
<211>  598
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  7
tatcttaact gatagtttga tcaaaggggc aaaacgtagg ggcaaacaaa cggaaaaatc       60

gtttctcaaa ttttctgatg ccaagaactc taaccagtct tatctaaaaa ttgccttatg      120

atccgtctct ccggttacag cctgtgtaac tgattaatcc tgcctttcta atcaccattc      180

taatgtttta attaagggat tttgtcttca ttaacggctt tcgctcataa aaatgttatg      240

acgttttgcc cgcaggcggg aaaccatcca cttcacgaga ctgatctcct ctgccggaac      300

accgggcatc tccaacttat aagttggaga aataagagaa tttcagattg agagaatgaa      360

aaaaaaaaaa aaaaaaggca gaggagagca tagaaatggg gttcactttt tggtaaagct      420

atagcatgcc tatcacatat aaatagagtg ccagtagcga cttttttcac actcgaaata      480

ctcttactac tgctctcttg ttgtttttat cacttcttgt ttcttcttgg taaatagaat      540

atcaagctac aaaaagcata caatcaacta tcaactatta actatatcgt aatacaca        598


<210>  8
<211>  998
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  8
aaccagggca aagcaaaata aaagaaactt aatacgttat gccgtaatga agggctacca       60

aaaacgataa tctcaactgt aaacaggtac aatgcggacc cttttgccac aaaacataca      120

tcattcattg ccggaaaaag aaagaagtga agacagcagt gcagccagcc atgttgcgcc      180

aatctaatta tagatgctgg tgccctgagg atgtatctgg agccagccat ggcatcatgc      240

gctaccgccg gatgtaaaat ccgacacgca aaagaaaacc ttcgaggttg cgcacttcgc      300

ccacccatga accacacggt tagtccaaaa ggggcagttc agattccaga tgcgggaatt      360

agcttgctgc caccctcacc tcactaacgc tgcggtgtgc ggatacttca tgctatttat      420

agacgcgcgt gtcggaatca gcacgcgcaa gaaccaaatg ggaaaatcgg aatgggtcca      480

gaactgcttt gagtgctggc tattggcgtc tgatttccgt tttgggaatc ctttgccgcg      540

cgcccctctc aaaactccgc acaagtccca gaaagcggga aagaaataaa acgccaccaa      600

aaaaaaaaat aaaagccaat cctcgaagcg tgggtggtag gccctggatt atcccgtaca      660

agtatttctc aggagtaaaa aaaccgtttg ttttggaatt ccccatttcg cggccaccta      720

cgccgctatc tttgcaacaa ctatctgcga taactcagca aattttgcat attcgtgttg      780

cagtattgcg ataatgggag tcttacttcc aacataacgg cagaaagaaa tgtgagaaaa      840

ttttgcatcc tttgcctccg ttcaagtata taaagtcggc atgcttgata atctttcttt      900

ccatcctaca ttgttctaat tattcttatt ctcctttatt ctttcctaac ataccaagaa      960

attaatcttc tgtcattcgc ttaaacacta tatcaata                              998


<210>  9
<211>  300
<212>  DNA
<213>  Saccharomyces cerevisiae

<400>  9
gaagttttgt tagaaaataa atcatttttt aattgagcat tcttattcct attttattta       60

aatagtttta tgtattgtta gctacataca acagtttaaa tcaaattttc tttttcccaa      120

gtccaaaatg gaggtttatt ttgatgaccc gcatgcgatt atgttttgaa agtataagac      180

tacatacatg tacatatatt taaacatgta aacccgtcca ttatattgct tactttcttc      240

ttttttgccg ttttgacttg gacctctggt ttgctatttc cttacaatct ttgctacaat      300


