                         LISTADO DE SECUENCIAS

<110>  Consejo Superior de Investigaciones Cientficas (CSIC)
 
<120>  SNTESIS DE BIODIESEL CATALIZADA POR UN CRUDO ENZIMTICO 
       INMOVILIZADO SOBRE PARTCULAS MAGNTICAS

<130>  ES1641.1245

<150>  P201730855
<151>  2017-06-28

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  1650
<212>  DNA
<213>  Ophiostoma piceae

<400>  1
atgcctaagc gcagagacca gtccatcgag ccccggacaa ccgtgaatgt aaactacccc       60

gagggtgagg ttgtcggtgt ttctgttctc ggtatcgagt ccttccgcgg tgtgccgttt      120

gctcagccac ccgtcggaaa cctgcgtctg aagccgcctg tccgctacac tgagaacatc      180

ggcaccaagg acaccactgg cattggcccg agttgtcccc agatgtacct gtctaccggc      240

aacggcgagc ttctgttcca gctcgtcggc aacctgatca acattcccct tttccagact      300

gccactctct cttccgagga ctgcctgacc ctcaacatcc agcggcccgc tggcaccaca      360

tccaacagca gcctgccggt gctcttctgg atctttggcg gcggcttcga gcttggcacc      420

aaccagtact atgatggcat cgatctgctg accgagggca tcagccttgg tgagccgttc      480

atctttgtgg ctatcaacta ccgtgttggt ggtttcggct tcctgggcgg caaggagatc      540

aaggctgacg ggtcgtctaa ccttggtctg ctggatcagc gcattgctct ggagtgggtg      600

gccgacaaca ttgcgtcgtt cggtggcgac ccgtccaagg tcaccatctg gggcgagtcg      660

gccggctcca tctccgtctt tgaccagatg gctctgtacg gtggcaacaa caagtacaag      720

ggcaaggctc tgttccgtgg tggcatcatg aactcgggca gtgtcgttcc tgctgccccc      780

gttgacggtg tcaaggccca ggccatctac gaccacgttg tcagcgaggc tggttgtgct      840

ggcacctctg acacccttgc gtgcctgcgc actgttgact acaccaagtt cctcactgcc      900

gtcaactcgg ttcccggaat tgtctcctac agctctatcg cactgtcgta cctgccgcgc      960

cccgatggtg tcgttcttat tgacagtccc gaggagattg ttaagaacaa gcagtacgcc     1020

gccgtgccca tgatcatcgg tgaccaggag gacgagggta cgctgtttgc cgtgctgccg     1080

aataacatca ccagcacggc caagattgtc cagtacttcc aggaccttta tttctataac     1140

gccaccaagg agcagctgac tgcctttgtc aacacctacc cgaccgatat caccgccggc     1200

tcccccttta acaccggcat cttcaacgag ctgtaccccg gtttcaagcg tctggctgcc     1260

attctcggtg acatgacgtt caccctggcg cgccgtgcct tcctgcagct ttgctccgag     1320

gtgaaccccg acgtgccctc gtggtcttac ctggcgtcgt acgactacgg cttccccttc     1380

ctcggcactt tccacgccac cgacatcctg caagtcttct atggcgtcct gcccaactat     1440

gcgagcggca gcatccagaa gtactacatc aacttcgtca ccactggtga tccgaacaag     1500

ggcgccgccg tcgacatcca gtggccgcaa tggagcgcga agaagaacat tctccagatc     1560

tatgccacca aggccgtgat cgtcgccgac aacttccgcg ccaagagcta cgagtacctc     1620

tacaacaaca ttggcatctt ccgcatctaa                                      1650


<210>  2
<211>  549
<212>  PRT
<213>  Ophiostoma piceae


<220>
<221>  SIGNAL
<222>  (1)..(12)

<220>
<221>  mat_peptide
<222>  (13)..(549)

<400>  2

Met Pro Lys Arg Arg Asp Gln Ser Ile Glu Pro Arg Thr Thr Val Asn 
        -10                 -5              -1  1               


Val Asn Tyr Pro Glu Gly Glu Val Val Gly Val Ser Val Leu Gly Ile 
5                   10                  15                  20  


Glu Ser Phe Arg Gly Val Pro Phe Ala Gln Pro Pro Val Gly Asn Leu 
                25                  30                  35      


Arg Leu Lys Pro Pro Val Arg Tyr Thr Glu Asn Ile Gly Thr Lys Asp 
            40                  45                  50          


Thr Thr Gly Ile Gly Pro Ser Cys Pro Gln Met Tyr Leu Ser Thr Gly 
        55                  60                  65              


Asn Gly Glu Leu Leu Phe Gln Leu Val Gly Asn Leu Ile Asn Ile Pro 
    70                  75                  80                  


Leu Phe Gln Thr Ala Thr Leu Ser Ser Glu Asp Cys Leu Thr Leu Asn 
85                  90                  95                  100 


Ile Gln Arg Pro Ala Gly Thr Thr Ser Asn Ser Ser Leu Pro Val Leu 
                105                 110                 115     


Phe Trp Ile Phe Gly Gly Gly Phe Glu Leu Gly Thr Asn Gln Tyr Tyr 
            120                 125                 130         


Asp Gly Ile Asp Leu Leu Thr Glu Gly Ile Ser Leu Gly Glu Pro Phe 
        135                 140                 145             


Ile Phe Val Ala Ile Asn Tyr Arg Val Gly Gly Phe Gly Phe Leu Gly 
    150                 155                 160                 


Gly Lys Glu Ile Lys Ala Asp Gly Ser Ser Asn Leu Gly Leu Leu Asp 
165                 170                 175                 180 


Gln Arg Ile Ala Leu Glu Trp Val Ala Asp Asn Ile Ala Ser Phe Gly 
                185                 190                 195     


Gly Asp Pro Ser Lys Val Thr Ile Trp Gly Glu Ser Ala Gly Ser Ile 
            200                 205                 210         


Ser Val Phe Asp Gln Met Ala Leu Tyr Gly Gly Asn Asn Lys Tyr Lys 
        215                 220                 225             


Gly Lys Ala Leu Phe Arg Gly Gly Ile Met Asn Ser Gly Ser Val Val 
    230                 235                 240                 


Pro Ala Ala Pro Val Asp Gly Val Lys Ala Gln Ala Ile Tyr Asp His 
245                 250                 255                 260 


Val Val Ser Glu Ala Gly Cys Ala Gly Thr Ser Asp Thr Leu Ala Cys 
                265                 270                 275     


Leu Arg Thr Val Asp Tyr Thr Lys Phe Leu Thr Ala Val Asn Ser Val 
            280                 285                 290         


Pro Gly Ile Val Ser Tyr Ser Ser Ile Ala Leu Ser Tyr Leu Pro Arg 
        295                 300                 305             


Pro Asp Gly Val Val Leu Ile Asp Ser Pro Glu Glu Ile Val Lys Asn 
    310                 315                 320                 


Lys Gln Tyr Ala Ala Val Pro Met Ile Ile Gly Asp Gln Glu Asp Glu 
325                 330                 335                 340 


Gly Thr Leu Phe Ala Val Leu Pro Asn Asn Ile Thr Ser Thr Ala Lys 
                345                 350                 355     


Ile Val Gln Tyr Phe Gln Asp Leu Tyr Phe Tyr Asn Ala Thr Lys Glu 
            360                 365                 370         


Gln Leu Thr Ala Phe Val Asn Thr Tyr Pro Thr Asp Ile Thr Ala Gly 
        375                 380                 385             


Ser Pro Phe Asn Thr Gly Ile Phe Asn Glu Leu Tyr Pro Gly Phe Lys 
    390                 395                 400                 


Arg Leu Ala Ala Ile Leu Gly Asp Met Thr Phe Thr Leu Ala Arg Arg 
405                 410                 415                 420 


Ala Phe Leu Gln Leu Cys Ser Glu Val Asn Pro Asp Val Pro Ser Trp 
                425                 430                 435     


Ser Tyr Leu Ala Ser Tyr Asp Tyr Gly Phe Pro Phe Leu Gly Thr Phe 
            440                 445                 450         


His Ala Thr Asp Ile Leu Gln Val Phe Tyr Gly Val Leu Pro Asn Tyr 
        455                 460                 465             


Ala Ser Gly Ser Ile Gln Lys Tyr Tyr Ile Asn Phe Val Thr Thr Gly 
    470                 475                 480                 


Asp Pro Asn Lys Gly Ala Ala Val Asp Ile Gln Trp Pro Gln Trp Ser 
485                 490                 495                 500 


Ala Lys Lys Asn Ile Leu Gln Ile Tyr Ala Thr Lys Ala Val Ile Val 
                505                 510                 515     


Ala Asp Asn Phe Arg Ala Lys Ser Tyr Glu Tyr Leu Tyr Asn Asn Ile 
            520                 525                 530         


Gly Ile Phe Arg Ile 
        535         


<210>  3
<211>  537
<212>  PRT
<213>  Ophiostoma piceae

<400>  3

Thr Thr Val Asn Val Asn Tyr Pro Glu Gly Glu Val Val Gly Val Ser 
1               5                   10                  15      


Val Leu Gly Ile Glu Ser Phe Arg Gly Val Pro Phe Ala Gln Pro Pro 
            20                  25                  30          


Val Gly Asn Leu Arg Leu Lys Pro Pro Val Arg Tyr Thr Glu Asn Ile 
        35                  40                  45              


Gly Thr Lys Asp Thr Thr Gly Ile Gly Pro Ser Cys Pro Gln Met Tyr 
    50                  55                  60                  


Leu Ser Thr Gly Asn Gly Glu Leu Leu Phe Gln Leu Val Gly Asn Leu 
65                  70                  75                  80  


Ile Asn Ile Pro Leu Phe Gln Thr Ala Thr Leu Ser Ser Glu Asp Cys 
                85                  90                  95      


Leu Thr Leu Asn Ile Gln Arg Pro Ala Gly Thr Thr Ser Asn Ser Ser 
            100                 105                 110         


Leu Pro Val Leu Phe Trp Ile Phe Gly Gly Gly Phe Glu Leu Gly Thr 
        115                 120                 125             


Asn Gln Tyr Tyr Asp Gly Ile Asp Leu Leu Thr Glu Gly Ile Ser Leu 
    130                 135                 140                 


Gly Glu Pro Phe Ile Phe Val Ala Ile Asn Tyr Arg Val Gly Gly Phe 
145                 150                 155                 160 


Gly Phe Leu Gly Gly Lys Glu Ile Lys Ala Asp Gly Ser Ser Asn Leu 
                165                 170                 175     


Gly Leu Leu Asp Gln Arg Ile Ala Leu Glu Trp Val Ala Asp Asn Ile 
            180                 185                 190         


Ala Ser Phe Gly Gly Asp Pro Ser Lys Val Thr Ile Trp Gly Glu Ser 
        195                 200                 205             


Ala Gly Ser Ile Ser Val Phe Asp Gln Met Ala Leu Tyr Gly Gly Asn 
    210                 215                 220                 


Asn Lys Tyr Lys Gly Lys Ala Leu Phe Arg Gly Gly Ile Met Asn Ser 
225                 230                 235                 240 


Gly Ser Val Val Pro Ala Ala Pro Val Asp Gly Val Lys Ala Gln Ala 
                245                 250                 255     


Ile Tyr Asp His Val Val Ser Glu Ala Gly Cys Ala Gly Thr Ser Asp 
            260                 265                 270         


Thr Leu Ala Cys Leu Arg Thr Val Asp Tyr Thr Lys Phe Leu Thr Ala 
        275                 280                 285             


Val Asn Ser Val Pro Gly Ile Val Ser Tyr Ser Ser Ile Ala Leu Ser 
    290                 295                 300                 


Tyr Leu Pro Arg Pro Asp Gly Val Val Leu Ile Asp Ser Pro Glu Glu 
305                 310                 315                 320 


Ile Val Lys Asn Lys Gln Tyr Ala Ala Val Pro Met Ile Ile Gly Asp 
                325                 330                 335     


Gln Glu Asp Glu Gly Thr Leu Phe Ala Val Leu Pro Asn Asn Ile Thr 
            340                 345                 350         


Ser Thr Ala Lys Ile Val Gln Tyr Phe Gln Asp Leu Tyr Phe Tyr Asn 
        355                 360                 365             


Ala Thr Lys Glu Gln Leu Thr Ala Phe Val Asn Thr Tyr Pro Thr Asp 
    370                 375                 380                 


Ile Thr Ala Gly Ser Pro Phe Asn Thr Gly Ile Phe Asn Glu Leu Tyr 
385                 390                 395                 400 


Pro Gly Phe Lys Arg Leu Ala Ala Ile Leu Gly Asp Met Thr Phe Thr 
                405                 410                 415     


Leu Ala Arg Arg Ala Phe Leu Gln Leu Cys Ser Glu Val Asn Pro Asp 
            420                 425                 430         


Val Pro Ser Trp Ser Tyr Leu Ala Ser Tyr Asp Tyr Gly Phe Pro Phe 
        435                 440                 445             


Leu Gly Thr Phe His Ala Thr Asp Ile Leu Gln Val Phe Tyr Gly Val 
    450                 455                 460                 


Leu Pro Asn Tyr Ala Ser Gly Ser Ile Gln Lys Tyr Tyr Ile Asn Phe 
465                 470                 475                 480 


Val Thr Thr Gly Asp Pro Asn Lys Gly Ala Ala Val Asp Ile Gln Trp 
                485                 490                 495     


Pro Gln Trp Ser Ala Lys Lys Asn Ile Leu Gln Ile Tyr Ala Thr Lys 
            500                 505                 510         


Ala Val Ile Val Ala Asp Asn Phe Arg Ala Lys Ser Tyr Glu Tyr Leu 
        515                 520                 525             


Tyr Asn Asn Ile Gly Ile Phe Arg Ile 
    530                 535         


<210>  4
<211>  543
<212>  PRT
<213>  Pichia pastoris

<400>  4

Glu Ala Tyr Val Glu Phe Thr Thr Val Asn Val Asn Tyr Pro Glu Gly 
1               5                   10                  15      


Glu Val Val Gly Val Ser Val Leu Gly Ile Glu Ser Phe Arg Gly Val 
            20                  25                  30          


Pro Phe Ala Gln Pro Pro Val Gly Asn Leu Arg Leu Lys Pro Pro Val 
        35                  40                  45              


Arg Tyr Thr Glu Asn Ile Gly Thr Lys Asp Thr Thr Gly Ile Gly Pro 
    50                  55                  60                  


Ser Cys Pro Gln Met Tyr Leu Ser Thr Gly Asn Gly Glu Leu Leu Phe 
65                  70                  75                  80  


Gln Leu Val Gly Asn Leu Ile Asn Ile Pro Leu Phe Gln Thr Ala Thr 
                85                  90                  95      


Leu Ser Ser Glu Asp Cys Leu Thr Leu Asn Ile Gln Arg Pro Ala Gly 
            100                 105                 110         


Thr Thr Ser Asn Ser Ser Leu Pro Val Leu Phe Trp Ile Phe Gly Gly 
        115                 120                 125             


Gly Phe Glu Leu Gly Thr Asn Gln Tyr Tyr Asp Gly Ile Asp Leu Leu 
    130                 135                 140                 


Thr Glu Gly Ile Ser Leu Gly Glu Pro Phe Ile Phe Val Ala Ile Asn 
145                 150                 155                 160 


Tyr Arg Val Gly Gly Phe Gly Phe Leu Gly Gly Lys Glu Ile Lys Ala 
                165                 170                 175     


Asp Gly Ser Ser Asn Leu Gly Leu Leu Asp Gln Arg Ile Ala Leu Glu 
            180                 185                 190         


Trp Val Ala Asp Asn Ile Ala Ser Phe Gly Gly Asp Pro Ser Lys Val 
        195                 200                 205             


Thr Ile Trp Gly Glu Ser Ala Gly Ser Ile Ser Val Phe Asp Gln Met 
    210                 215                 220                 


Ala Leu Tyr Gly Gly Asn Asn Lys Tyr Lys Gly Lys Ala Leu Phe Arg 
225                 230                 235                 240 


Gly Gly Ile Met Asn Ser Gly Ser Val Val Pro Ala Ala Pro Val Asp 
                245                 250                 255     


Gly Val Lys Ala Gln Ala Ile Tyr Asp His Val Val Ser Glu Ala Gly 
            260                 265                 270         


Cys Ala Gly Thr Ser Asp Thr Leu Ala Cys Leu Arg Thr Val Asp Tyr 
        275                 280                 285             


Thr Lys Phe Leu Thr Ala Val Asn Ser Val Pro Gly Ile Val Ser Tyr 
    290                 295                 300                 


Ser Ser Ile Ala Leu Ser Tyr Leu Pro Arg Pro Asp Gly Val Val Leu 
305                 310                 315                 320 


Ile Asp Ser Pro Glu Glu Ile Val Lys Asn Lys Gln Tyr Ala Ala Val 
                325                 330                 335     


Pro Met Ile Ile Gly Asp Gln Glu Asp Glu Gly Thr Leu Phe Ala Val 
            340                 345                 350         


Leu Pro Asn Asn Ile Thr Ser Thr Ala Lys Ile Val Gln Tyr Phe Gln 
        355                 360                 365             


Asp Leu Tyr Phe Tyr Asn Ala Thr Lys Glu Gln Leu Thr Ala Phe Val 
    370                 375                 380                 


Asn Thr Tyr Pro Thr Asp Ile Thr Ala Gly Ser Pro Phe Asn Thr Gly 
385                 390                 395                 400 


Ile Phe Asn Glu Leu Tyr Pro Gly Phe Lys Arg Leu Ala Ala Ile Leu 
                405                 410                 415     


Gly Asp Met Thr Phe Thr Leu Ala Arg Arg Ala Phe Leu Gln Leu Cys 
            420                 425                 430         


Ser Glu Val Asn Pro Asp Val Pro Ser Trp Ser Tyr Leu Ala Ser Tyr 
        435                 440                 445             


Asp Tyr Gly Phe Pro Phe Leu Gly Thr Phe His Ala Thr Asp Ile Leu 
    450                 455                 460                 


Gln Val Phe Tyr Gly Val Leu Pro Asn Tyr Ala Ser Gly Ser Ile Gln 
465                 470                 475                 480 


Lys Tyr Tyr Ile Asn Phe Val Thr Thr Gly Asp Pro Asn Lys Gly Ala 
                485                 490                 495     


Ala Val Asp Ile Gln Trp Pro Gln Trp Ser Ala Lys Lys Asn Ile Leu 
            500                 505                 510         


Gln Ile Tyr Ala Thr Lys Ala Val Ile Val Ala Asp Asn Phe Arg Ala 
        515                 520                 525             


Lys Ser Tyr Glu Tyr Leu Tyr Asn Asn Ile Gly Ile Phe Arg Ile 
    530                 535                 540             


<210>  5
<211>  545
<212>  PRT
<213>  Pichia pastoris

<400>  5

Glu Ala Glu Ala Tyr Val Glu Phe Thr Thr Val Asn Val Asn Tyr Pro 
1               5                   10                  15      


Glu Gly Glu Val Val Gly Val Ser Val Leu Gly Ile Glu Ser Phe Arg 
            20                  25                  30          


Gly Val Pro Phe Ala Gln Pro Pro Val Gly Asn Leu Arg Leu Lys Pro 
        35                  40                  45              


Pro Val Arg Tyr Thr Glu Asn Ile Gly Thr Lys Asp Thr Thr Gly Ile 
    50                  55                  60                  


Gly Pro Ser Cys Pro Gln Met Tyr Leu Ser Thr Gly Asn Gly Glu Leu 
65                  70                  75                  80  


Leu Phe Gln Leu Val Gly Asn Leu Ile Asn Ile Pro Leu Phe Gln Thr 
                85                  90                  95      


Ala Thr Leu Ser Ser Glu Asp Cys Leu Thr Leu Asn Ile Gln Arg Pro 
            100                 105                 110         


Ala Gly Thr Thr Ser Asn Ser Ser Leu Pro Val Leu Phe Trp Ile Phe 
        115                 120                 125             


Gly Gly Gly Phe Glu Leu Gly Thr Asn Gln Tyr Tyr Asp Gly Ile Asp 
    130                 135                 140                 


Leu Leu Thr Glu Gly Ile Ser Leu Gly Glu Pro Phe Ile Phe Val Ala 
145                 150                 155                 160 


Ile Asn Tyr Arg Val Gly Gly Phe Gly Phe Leu Gly Gly Lys Glu Ile 
                165                 170                 175     


Lys Ala Asp Gly Ser Ser Asn Leu Gly Leu Leu Asp Gln Arg Ile Ala 
            180                 185                 190         


Leu Glu Trp Val Ala Asp Asn Ile Ala Ser Phe Gly Gly Asp Pro Ser 
        195                 200                 205             


Lys Val Thr Ile Trp Gly Glu Ser Ala Gly Ser Ile Ser Val Phe Asp 
    210                 215                 220                 


Gln Met Ala Leu Tyr Gly Gly Asn Asn Lys Tyr Lys Gly Lys Ala Leu 
225                 230                 235                 240 


Phe Arg Gly Gly Ile Met Asn Ser Gly Ser Val Val Pro Ala Ala Pro 
                245                 250                 255     


Val Asp Gly Val Lys Ala Gln Ala Ile Tyr Asp His Val Val Ser Glu 
            260                 265                 270         


Ala Gly Cys Ala Gly Thr Ser Asp Thr Leu Ala Cys Leu Arg Thr Val 
        275                 280                 285             


Asp Tyr Thr Lys Phe Leu Thr Ala Val Asn Ser Val Pro Gly Ile Val 
    290                 295                 300                 


Ser Tyr Ser Ser Ile Ala Leu Ser Tyr Leu Pro Arg Pro Asp Gly Val 
305                 310                 315                 320 


Val Leu Ile Asp Ser Pro Glu Glu Ile Val Lys Asn Lys Gln Tyr Ala 
                325                 330                 335     


Ala Val Pro Met Ile Ile Gly Asp Gln Glu Asp Glu Gly Thr Leu Phe 
            340                 345                 350         


Ala Val Leu Pro Asn Asn Ile Thr Ser Thr Ala Lys Ile Val Gln Tyr 
        355                 360                 365             


Phe Gln Asp Leu Tyr Phe Tyr Asn Ala Thr Lys Glu Gln Leu Thr Ala 
    370                 375                 380                 


Phe Val Asn Thr Tyr Pro Thr Asp Ile Thr Ala Gly Ser Pro Phe Asn 
385                 390                 395                 400 


Thr Gly Ile Phe Asn Glu Leu Tyr Pro Gly Phe Lys Arg Leu Ala Ala 
                405                 410                 415     


Ile Leu Gly Asp Met Thr Phe Thr Leu Ala Arg Arg Ala Phe Leu Gln 
            420                 425                 430         


Leu Cys Ser Glu Val Asn Pro Asp Val Pro Ser Trp Ser Tyr Leu Ala 
        435                 440                 445             


Ser Tyr Asp Tyr Gly Phe Pro Phe Leu Gly Thr Phe His Ala Thr Asp 
    450                 455                 460                 


Ile Leu Gln Val Phe Tyr Gly Val Leu Pro Asn Tyr Ala Ser Gly Ser 
465                 470                 475                 480 


Ile Gln Lys Tyr Tyr Ile Asn Phe Val Thr Thr Gly Asp Pro Asn Lys 
                485                 490                 495     


Gly Ala Ala Val Asp Ile Gln Trp Pro Gln Trp Ser Ala Lys Lys Asn 
            500                 505                 510         


Ile Leu Gln Ile Tyr Ala Thr Lys Ala Val Ile Val Ala Asp Asn Phe 
        515                 520                 525             


Arg Ala Lys Ser Tyr Glu Tyr Leu Tyr Asn Asn Ile Gly Ile Phe Arg 
    530                 535                 540                 


Ile 
545 


