﻿               SEQUENCE LISTING

<110> PARIS SCIENCES ET LETTRES - QUARTIER LATIN
      CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
      INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCHE MÉDICALE
      TOPILKO Piotr
      CHARNAY Patrick
      COULPIER Fanny
      GRESSET Aurélie
      RADOMSKA Katarzyna

<120> NON-HUMAN MODEL FOR NEUROFIBROMATOSIS TYPE 1

<130> CV - 716/PCT

<160> 18

<170> BiSSAP 1.3.6

<210> 1
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Prss56 primer (Forward)

<400> 1
gtcacaatcc ccgagaacag                                                20


<210> 2
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Prss56 primer (Reverse)

<400> 2
ctcgctgaat agccgctaac                                                20


<210> 3
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Prss56 primer (Forward)

<400> 3
ggtcttcagt ggcctagtgg                                                20


<210> 4
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Prss56 primer (Reverse)

<400> 4
agcctctgtc cttgatcagc                                                20


<210> 5
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Prss56 primer (Forward)

<400> 5
cctagcgctc caacagtgtc                                                20


<210> 6
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Prss56 primer (Reverse)

<400> 6
agtgctcagc tgggatctgt                                                20


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Prss56 primer (Forward)

<400> 7
atgacctggc cttggtacag                                                20


<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Prss56 primer (Reverse)

<400> 8
gacaggtgtc tgcactgagc                                                20


<210> 9
<211> 343
<212> PRT
<213> Enterobacteria phage P1


<220> 
<223> Cre recombinase

<400> 9
Met Ser Asn Leu Leu Thr Val His Gln Asn Leu Pro Ala Leu Pro Val 
1               5                   10                  15      
Asp Ala Thr Ser Asp Glu Val Arg Lys Asn Leu Met Asp Met Phe Arg 
            20                  25                  30          
Asp Arg Gln Ala Phe Ser Glu His Thr Trp Lys Met Leu Leu Ser Val 
        35                  40                  45              
Cys Arg Ser Trp Ala Ala Trp Cys Lys Leu Asn Asn Arg Lys Trp Phe 
    50                  55                  60                  
Pro Ala Glu Pro Glu Asp Val Arg Asp Tyr Leu Leu Tyr Leu Gln Ala 
65                  70                  75                  80  
Arg Gly Leu Ala Val Lys Thr Ile Gln Gln His Leu Gly Gln Leu Asn 
                85                  90                  95      
Met Leu His Arg Arg Ser Gly Leu Pro Arg Pro Ser Asp Ser Asn Ala 
            100                 105                 110         
Val Ser Leu Val Met Arg Arg Ile Arg Lys Glu Asn Val Asp Ala Gly 
        115                 120                 125             
Glu Arg Ala Lys Gln Ala Leu Ala Phe Glu Arg Thr Asp Phe Asp Gln 
    130                 135                 140                 
Val Arg Ser Leu Met Glu Asn Ser Asp Arg Cys Gln Asp Ile Arg Asn 
145                 150                 155                 160 
Leu Ala Phe Leu Gly Ile Ala Tyr Asn Thr Leu Leu Arg Ile Ala Glu 
                165                 170                 175     
Ile Ala Arg Ile Arg Val Lys Asp Ile Ser Arg Thr Asp Gly Gly Arg 
            180                 185                 190         
Met Leu Ile His Ile Gly Arg Thr Lys Thr Leu Val Ser Thr Ala Gly 
        195                 200                 205             
Val Glu Lys Ala Leu Ser Leu Gly Val Thr Lys Leu Val Glu Arg Trp 
    210                 215                 220                 
Ile Ser Val Ser Gly Val Ala Asp Asp Pro Asn Asn Tyr Leu Phe Cys 
225                 230                 235                 240 
Arg Val Arg Lys Asn Gly Val Ala Ala Pro Ser Ala Thr Ser Gln Leu 
                245                 250                 255     
Ser Thr Arg Ala Leu Glu Gly Ile Phe Glu Ala Thr His Arg Leu Ile 
            260                 265                 270         
Tyr Gly Ala Lys Asp Asp Ser Gly Gln Arg Tyr Leu Ala Trp Ser Gly 
        275                 280                 285             
His Ser Ala Arg Val Gly Ala Ala Arg Asp Met Ala Arg Ala Gly Val 
    290                 295                 300                 
Ser Ile Pro Glu Ile Met Gln Ala Gly Gly Trp Thr Asn Val Asn Ile 
305                 310                 315                 320 
Val Met Asn Tyr Ile Arg Asn Leu Asp Ser Glu Thr Gly Ala Met Val 
                325                 330                 335     
Arg Leu Leu Glu Asp Gly Asp 
            340             

<210> 10
<211> 1053
<212> DNA
<213> Artificial Sequence


<220> 
<223> Cre recombinase (cDNA)

<400> 10
atgcccaaga agaagaggaa ggtgtccaat ttactgaccg tacaccaaaa tttgcctgca      60

ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc tgatggacat gttcagggat     120

cgccaggcgt tttctgagca tacctggaaa atgcttctgt ccgtttgccg gtcgtgggcg     180

gcatggtgca agttgaataa ccggaaatgg tttcccgcag aacctgaaga tgttcgcgat     240

tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa ctatccagca acatttgggc     300

cagctaaaca tgcttcatcg tcggtccggg ctgccacgac caagtgacag caatgctgtt     360

tcactggtta tgcggcggat ccgaaaagaa aacgttgatg ccggtgaacg tgcaaaacag     420

gctctagcgt tcgaacgcac tgatttcgac caggttcgtt cactcatgga aaatagcgat     480

cgctgccagg atatacgtaa tctggcattt ctggggattg cttataacac cctgttacgt     540

atagccgaaa ttgccaggat cagggttaaa gatatctcac gtactgacgg tgggagaatg     600

ttaatccata ttggcagaac gaaaacgctg gttagcaccg caggtgtaga gaaggcactt     660

agcctggagg taactaaact ggtcgagcga tggatttccg tctctggtgt agctgatgat     720

ccgaataact acctgttttg ccgggtcaga aaaaatggtg ttgccgcgcc atctgccacc     780

agccagctat caactcgcgc cctggaaggg atttttgaag caactcatcg attgatttac     840

ggcgctaagg atgactctgg tcagagatac ctggcctggt ctggacacag tgcccgtgtc     900

ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat gcaagctggt     960

ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag tgaaacaggg    1020

gcaatggtgc gcctgctgga agatggcgat tag                                 1053


<210> 11
<211> 34
<212> DNA
<213> Enterobacteria phage P1


<220> 
<223> loxP

<220> 
<221> variation
<222> 14
<223> /note="n is any of A, C, G or T"

<220> 
<221> variation
<222> 15
<223> /note="n is any of A, C, G or T"

<220> 
<221> variation
<222> 16
<223> /note="n is any of A, C, G or T"

<220> 
<221> variation
<222> 19
<223> /note="n is any of A, C, G or T"

<220> 
<221> variation
<222> 20
<223> /note="n is any of A, C, G or T"

<220> 
<221> variation
<222> 21
<223> /note="n is any of A, C, G or T"

<400> 11
ataacttcgt atannntann ntatacgaag ttat                                 34


<210> 12
<211> 34
<212> DNA
<213> Enterobacteria phage P1


<220> 
<223> loxP

<400> 12
ataacttcgt ataatgtatg ctatacgaag ttat                                 34


<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Prss56Cre knock-in primer (Forward)

<400> 13
caggtgaggt gcggaccatt                                                20


<210> 14
<211> 25
<212> DNA
<213> Artificial Sequence


<220> 
<223> Prss56Cre knock-in primer (Reverse)

<400> 14
acggaaatcc atcgctcgac cagtt                                          25


<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Beta-actin primer (forward)

<400> 15
tgttaccaac tgggacgaca                                                20


<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Beta-actin primer (reverse)

<400> 16
ggggtgttga aggtctcaaa                                                20


<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nf1 primer (forward)

<400> 17
gcttccctca gaacagcatc                                                20


<210> 18
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nf1 primer (reverse)

<400> 18
gcccctttca attctaggtg g                                              21


