                         SEQUENCE LISTING

<110>  Technische Universitt Dresden
 
<120>  Enzyme und Verfahren zur stereoselektiven Reduktion von 
       Carbonylverbindungen, Oxidation sowie stereoselektiven reduktiven
        Aminierung - zur enantioslektiven Darstellung von 
       Alkoholaminverbidnungen

<130>  00017P0318DEWO

<150>  DE102017210944.0
<151>  2017-06-28

<160>  13    

<170>  PatentIn version 3.5

<210>  1
<211>  249
<212>  PRT
<213>  Arthrobacter aurescens

<400>  1

Met Leu Val Glu Gly Lys Asn Gly Ile Val Thr Gly Ala Ala Gly Gly 
1               5                   10                  15      


Ile Gly Arg Ala Ser Ala Val Ala Phe Ala Arg Glu Gly Ala Asn Val 
            20                  25                  30          


Val Ile Gly Asp Leu Gln Ala Arg Leu Glu Asp Leu Glu Glu Thr Ala 
        35                  40                  45              


Arg Gln Val Lys Lys Ala Gly Gly Glu Ala Arg Val Leu Val Met Asp 
    50                  55                  60                  


Val Thr Ala Leu Ala Asp Gln Glu His Ala Val Arg Thr Val Ile Glu 
65                  70                  75                  80  


Asn Phe Gly Thr Leu Asp Phe Ala His Asn Asn Ala Gly Ile Glu Leu 
                85                  90                  95      


Gln Lys Thr Ala Leu Glu Thr Thr Glu Glu Glu Trp Asp Arg Val His 
            100                 105                 110         


Asp Val Asn Leu Lys Gly Val Phe Leu Gly Met Lys Ala Gln Leu Arg 
        115                 120                 125             


Ala Met Ile Asp Asn Gly Gly Gly Ser Ile Val Asn Thr Ala Ser Ala 
    130                 135                 140                 


Ala Gly Ile Leu Gly Leu Pro Gly Tyr Ser Gly Tyr Ala Ser Ser Lys 
145                 150                 155                 160 


His Gly Val Val Gly Leu Thr Lys Ser Val Ala Val Glu Ala Ala Asp 
                165                 170                 175     


Thr Gly Val Arg Ile Asn Ala Val Cys Pro Ala Ser Ile Ala Thr Pro 
            180                 185                 190         


Met Leu Leu Ser Leu Pro Ala Glu Glu Gln Glu Thr Leu Leu Ser Trp 
        195                 200                 205             


Gln Ala Ile Lys Arg Leu Gly Lys Pro Asp Glu Val Ala Gln Ala Val 
    210                 215                 220                 


Val Trp Leu Ala Ser Asp Arg Ala Ser Phe Ile Thr Gly Val Ser Leu 
225                 230                 235                 240 


Pro Val Asp Ala Gly Tyr Ser Ala Phe 
                245                 


<210>  2
<211>  274
<212>  PRT
<213>  Arthrobacter aurescens

<400>  2

Met Ile Asn Met Arg Asn Arg Val Ala Ile Val Thr Gly Gly Ala Met 
1               5                   10                  15      


Gly Met Gly Asn Gly Cys Ala Arg Lys Leu Ala Glu Ala Gly Ala Lys 
            20                  25                  30          


Val Tyr Leu Ile Asp Arg Ser Asn Leu Val Ala Ala Ala Ala Gln Asp 
        35                  40                  45              


Met Arg Glu Ala Gly Leu Asn Ala Asn His Val Gln Val Asp Ile Thr 
    50                  55                  60                  


Asp Gln Glu Ser Leu Thr Ser Ala Tyr Asp Gly Ile Ala Ala Glu Ser 
65                  70                  75                  80  


Gly Arg Leu Asp Val Leu Val Asn Ala Ala Gly Val Gly Asp Ser Arg 
                85                  90                  95      


Met Phe Leu Asp Val Asp Asp Ala His Tyr Lys Lys Val Ile Asp Val 
            100                 105                 110         


Asn Val Arg Gly Thr Trp Asn Ser Cys Arg Ala Ala Val Pro His Met 
        115                 120                 125             


Leu Ser Asn Lys His Gly Arg Ile Ile Asn Phe Gly Ser Ile Ser Gly 
    130                 135                 140                 


Pro Ile Val Ala Asp Pro Gly Trp Thr Val Tyr Ala Leu Ser Lys Gly 
145                 150                 155                 160 


Ala Ile Phe Gly Phe Thr Lys Ala Leu Ala Ser Glu Phe Ala Gly Gln 
                165                 170                 175     


Asn Ile Leu Val Asn Ala Ile Leu Pro Gly Ser Met Asp Thr Pro Met 
            180                 185                 190         


Met Arg Ala Ala Ala Ala Asp Thr Asn Pro Ala Asp Pro Gln Ser Val 
        195                 200                 205             


Ile Asp Glu Ile Ala Ala Ala Val Pro Leu Lys Arg Leu Gly Thr Ile 
    210                 215                 220                 


Asp Asp Ala Gly Asn Leu Ala Leu Phe Leu Ala Ser Asp Leu Ala Ser 
225                 230                 235                 240 


Tyr Leu Thr Gly Gln Ala Ile Val Leu Asp Gly Ala Phe Thr Leu Ala 
                245                 250                 255     


Glu Tyr Gln Ser Gly Gly Leu Glu Ala Pro Ala Glu Thr Pro Ala Leu 
            260                 265                 270         


Val Asn 
        


<210>  3
<211>  19
<212>  PRT
<213>  Arthrobacter aurescens

<400>  3

Ala Ser Ala Ala Gly Ile Leu Gly Leu Pro Gly Tyr Ser Gly Tyr Ala 
1               5                   10                  15      


Ser Ser Lys 
            


<210>  4
<211>  29
<212>  PRT
<213>  Arthrobacter aurescens

<400>  4

Pro Ala Ser Ile Ala Thr Pro Met Leu Leu Ser Leu Pro Ala Glu Glu 
1               5                   10                  15      


Gln Glu Thr Leu Leu Ser Trp Gln Ala Ile Lys Arg Leu 
            20                  25                  


<210>  5
<211>  6
<212>  PRT
<213>  Arthrobacter aurescens

<400>  5

Val Asp Ala Gly Tyr Ser 
1               5       


<210>  6
<211>  22
<212>  PRT
<213>  Arthrobacter aurescens

<400>  6

Arg Ile Ile Asn Phe Gly Ser Ile Ser Gly Pro Ile Val Ala Asp Pro 
1               5                   10                  15      


Gly Trp Thr Val Tyr Ala 
            20          


<210>  7
<211>  39
<212>  PRT
<213>  Arthrobacter aurescens

<400>  7

Ala Ile Leu Pro Gly Ser Met Asp Thr Pro Met Met Arg Ala Ala Ala 
1               5                   10                  15      


Ala Asp Thr Asn Pro Ala Asp Pro Gln Ser Val Ile Asp Glu Ile Ala 
            20                  25                  30          


Ala Ala Val Pro Leu Lys Arg 
        35                  


<210>  8
<211>  10
<212>  PRT
<213>  Arthrobacter aurescens

<400>  8

Ala Ile Val Leu Asp Gly Ala Phe Thr Leu 
1               5                   10  


<210>  9
<211>  364
<212>  PRT
<213>  Candida boidinii

<400>  9

Met Lys Ile Val Leu Val Leu Tyr Asp Ala Gly Lys His Ala Ala Asp 
1               5                   10                  15      


Glu Glu Lys Leu Tyr Gly Cys Thr Glu Asn Lys Leu Gly Ile Ala Asn 
            20                  25                  30          


Trp Leu Lys Asp Gln Gly His Glu Leu Ile Thr Thr Ser Asp Lys Glu 
        35                  40                  45              


Gly Glu Thr Ser Glu Leu Asp Lys His Ile Pro Asp Ala Asp Ile Ile 
    50                  55                  60                  


Ile Thr Thr Pro Phe His Pro Ala Tyr Ile Thr Lys Glu Arg Leu Asp 
65                  70                  75                  80  


Lys Ala Lys Asn Leu Lys Leu Val Val Val Ala Gly Val Gly Ser Asp 
                85                  90                  95      


His Ile Asp Leu Asp Tyr Ile Asn Gln Thr Gly Lys Lys Ile Ser Val 
            100                 105                 110         


Leu Glu Val Thr Gly Ser Asn Val Val Ser Val Ala Glu His Val Val 
        115                 120                 125             


Met Thr Met Leu Val Leu Val Arg Asn Phe Val Pro Ala His Glu Gln 
    130                 135                 140                 


Ile Ile Asn His Asp Trp Glu Val Ala Ala Ile Ala Lys Asp Ala Tyr 
145                 150                 155                 160 


Asp Ile Glu Gly Lys Thr Ile Ala Thr Ile Gly Ala Gly Arg Ile Gly 
                165                 170                 175     


Tyr Arg Val Leu Glu Arg Leu Leu Pro Phe Asn Pro Lys Glu Leu Leu 
            180                 185                 190         


Tyr Tyr Asp Tyr Gln Ala Leu Pro Lys Glu Ala Glu Glu Lys Val Gly 
        195                 200                 205             


Ala Arg Arg Val Glu Asn Ile Glu Glu Leu Val Ala Gln Ala Asp Ile 
    210                 215                 220                 


Val Thr Val Asn Ala Pro Leu His Ala Gly Thr Lys Gly Leu Ile Asn 
225                 230                 235                 240 


Lys Glu Leu Leu Ser Lys Phe Lys Lys Gly Ala Trp Leu Val Asn Thr 
                245                 250                 255     


Ala Arg Gly Ala Ile Cys Val Ala Glu Asp Val Ala Ala Ala Leu Glu 
            260                 265                 270         


Ser Gly Gln Leu Arg Gly Tyr Gly Gly Asp Val Trp Phe Pro Gln Pro 
        275                 280                 285             


Ala Pro Lys Asp His Pro Trp Arg Asp Met Arg Asn Lys Tyr Gly Ala 
    290                 295                 300                 


Gly Asn Ala Met Thr Pro His Tyr Ser Gly Thr Thr Leu Asp Ala Gln 
305                 310                 315                 320 


Thr Arg Tyr Ala Glu Gly Thr Lys Asn Ile Leu Glu Ser Phe Phe Thr 
                325                 330                 335     


Gly Lys Phe Asp Tyr Arg Pro Gln Asp Ile Ile Leu Leu Asn Gly Glu 
            340                 345                 350         


Tyr Val Thr Lys Ala Tyr Gly Lys His Asp Lys Lys 
        355                 360                 


<210>  10
<211>  750
<212>  DNA
<213>  Arthrobacter aurescens

<400>  10
atgctggttg aaggaaaaaa cggcatcgtg accggtgctg ccggtggtat cggccgcgcc       60

tcggcggtcg catttgcacg tgaaggcgcc aacgtggtca tcggagacct acaggcgcgg      120

ctggaggact tggaagaaac ggcccgccag gtcaaaaaag ccgggggaga agcgcgcgtg      180

ctggtgatgg acgttaccgc actggccgat caggagcatg ccgtcaggac ggtcattgaa      240

aattttggca ctctggattt cgcccacaac aacgccggta tcgagcttca gaagaccgcg      300

ttggagacga ccgaagaaga gtgggaccgc gtccacgacg ttaatttgaa gggcgtcttc      360

ctcgggatga aggcacaact tcgggccatg atcgacaacg gcgggggctc gatcgtaaac      420

accgccagcg ccgcaggaat tctcgggttg cctggatact ccggctacgc ctcatcgaag      480

catggtgttg tgggcctcac taaatccgta gccgtggagg cagcagacac gggcgttcgc      540

atcaatgctg tgtgcccggc ttcaatcgcc acgcccatgc ttctgtccct accggcggag      600

gagcaggaaa ctttgctctc atggcaagca attaaacgat tgggtaaacc ggacgaggtt      660

gcccaggcag tcgtgtggct tgcctccgac cgtgcgtcct tcatcaccgg cgtcagcctc      720

cccgtagacg ctggatactc ggctttctga                                       750


<210>  11
<211>  825
<212>  DNA
<213>  Arthrobacter aurescens

<400>  11
atgatcaata tgcgaaacag ggtcgccatc gtgactggtg gtgcgatggg catgggtaac       60

ggctgtgccc ggaaactggc cgaagccggt gccaaggttt acctcattga caggtcaaac      120

cttgtagcag ccgctgccca ggacatgcgc gaggcagggc tgaacgccaa ccacgttcag      180

gtagacatca cagaccagga atccctcact tccgcgtacg acgggattgc agcggagtcc      240

ggcaggctag acgtcctggt aaacgccgcc ggagtcggtg actcgcggat gttcctggac      300

gtcgatgacg cccactacaa aaaagtcatc gacgtgaacg tccgaggcac ctggaactct      360

tgccgggccg ctgtcccgca catgctgtcc aacaaacacg gcaggattat caacttcggg      420

tcaatctccg ggcccatcgt tgccgacccg ggctggactg tctacgcact gtccaagggt      480

gctatcttcg gtttcaccaa agccctggct tccgaatttg cgggacagaa catcctggta      540

aacgcgatcc tcccaggcag catggacact cccatgatgc gggcagccgc cgcagacacg      600

aacccagcgg atccccaaag tgtcatcgac gaaatcgctg cagccgttcc cctcaaacga      660

ctgggcacca tcgacgacgc tggcaacctg gcactgttcc tcgcatcaga cctggcaagc      720

tacctcacag gtcaggccat cgtcttggat ggggccttca ctctcgcgga gtaccagtcg      780

ggcggcctgg aagcgcccgc ggagaccccc gctctcgtca actaa                      825


<210>  12
<211>  1095
<212>  DNA
<213>  Candida boidinii

<400>  12
atgaagattg tcttagttct ttatgatgct ggtaagcacg ctgctgatga agaaaaatta       60

tatggttgta ctgaaaataa attaggtatt gccaattggt taaaagatca aggtcatgaa      120

ctaattacta cttctgataa agaaggtgaa acaagcgaat tggataaaca tatcccagat      180

gctgatatta tcatcaccac tcctttccat cctgcttata tcactaagga aagacttgac      240

aaggctaaga acttaaaatt agtcgttgtc gctggtgttg gttctgatca cattgattta      300

gattatatta atcaaacagg taagaaaatc tcagtcctgg aagttacagg ttctaatgtt      360

gtctctgttg ctgaacacgt tgtcatgacc atgcttgtct tggttagaaa tttcgttcca      420

gcacatgaac aaattattaa ccacgattgg gaggttgctg ctatcgctaa ggatgcttac      480

gatatcgaag gtaaaactat cgctaccatt ggtgctggta gaattggtta cagagtcttg      540

gaaagattac tcccatttaa tccaaaagaa ttattatact acgattatca agctttacca      600

aaagaagctg aagaaaaagt tggtgctaga agagttgaaa atattgaaga attagttgct      660

caagctgata tcgttacagt taatgctcca ttacacgcag gtacaaaagg tttaattaat      720

aaggaattat tatctaaatt taaaaaaggt gcttggttag tcaataccgc aagaggtgct      780

atttgtgttg ctgaagatgt tgcagcagct ttagaatctg gtcaattaag aggttacggt      840

ggtgatgttt ggttcccaca accagctcca aaggatcacc catggagaga tatgagaaat      900

aaatatggtg ctggtaatgc catgactcct cactactctg gtactacttt agacgctcaa      960

acaagatacg ctgaaggtac taaaaatatt ttggaatcat tctttaccgg taaatttgat     1020

tacagaccac aagatattat cttattaaat ggggaatacg ttactaaagc ttacggtaaa     1080

cacgataaga aatag                                                      1095


<210>  13
<211>  372
<212>  PRT
<213>  Arthrobacter aurescens

<400>  13

Met Ile Ile Gly Val Pro Lys Glu Ile Lys Asn Asn Glu Phe Arg Val 
1               5                   10                  15      


Ala Ile Thr Ala Ala Gly Val His Glu Phe Arg Thr His Gly His Ser 
            20                  25                  30          


Val Leu Val Glu Arg Gly Ala Gly Leu Gly Ser Gly Ile Thr Asp Glu 
        35                  40                  45              


Glu Tyr Ser Ile Ala Gly Ala Glu Ile Val Asn Glu Ala Asp Asp Val 
    50                  55                  60                  


Trp Ala Arg Ala Asp Met Val Met Lys Val Lys Glu Pro Ile Ala Ala 
65                  70                  75                  80  


Glu Tyr His Arg Phe Arg Lys Gly Leu Ile Leu Phe Thr Tyr Leu His 
                85                  90                  95      


Leu Ala Ala Glu Pro Glu Leu Thr Ala Glu Leu Ile Asn Ser Gly Val 
            100                 105                 110         


Thr Ala Ile Ala Tyr Glu Thr Val Gln Glu Gly Arg Thr Leu Pro Leu 
        115                 120                 125             


Leu Ala Pro Met Ser Glu Val Ala Gly Arg Leu Ser Val Val Val Gly 
    130                 135                 140                 


Ala Ser Ser Leu Met Ala Pro Ala Gly Gly Lys Gly Val Leu Leu Gly 
145                 150                 155                 160 


Gly Val Pro Gly Val Arg Pro Ala Lys Val Val Val Leu Gly Ala Gly 
                165                 170                 175     


Val Ala Gly Thr Asn Ala Ala Ala Met Ala Leu Gly Leu Gly Ala Asp 
            180                 185                 190         


Val Thr Ile Met Asp Ile Asn Ile Asn Arg Leu Arg Glu Leu Asp Ala 
        195                 200                 205             


Leu Tyr Gln Gly Arg Leu Lys Thr Val Ala Ser Asn Ala Tyr Glu Ile 
    210                 215                 220                 


Glu Lys Ser Val Ile Asp Ala Asp Leu Val Ile Gly Ser Val Leu Ile 
225                 230                 235                 240 


Pro Gly Ala Lys Ala Pro Lys Leu Val Thr Asn Asp Leu Val Ala Arg 
                245                 250                 255     


Met Lys Pro Gly Ser Val Leu Val Asp Ile Ala Val Asp Gln Gly Gly 
            260                 265                 270         


Cys Phe Glu Asp Thr His Pro Thr Thr His Gln Glu Pro Thr Tyr Lys 
        275                 280                 285             


Val His Asn Ser Ile Phe Tyr Cys Val Ala Asn Met Pro Gly Ala Val 
    290                 295                 300                 


Pro Asn Thr Ser Thr Tyr Ala Leu Thr Asn Val Thr Leu Arg Tyr Ala 
305                 310                 315                 320 


Val Ala Leu Ala Asn Leu Gly Val Lys Ala Ala Phe Asp Arg Asp Pro 
                325                 330                 335     


Ala Leu Ala Ala Gly Leu Asn Ile Ala Ala Gly His Val Ala His His 
            340                 345                 350         


Ser Val Ser Glu Ala His Asn Leu Pro Leu Val Thr Asp Trp His Ser 
        355                 360                 365             


Leu Val Thr Ala 
    370         


